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C Vijayakumar, M Ramesh, A Murugesan, N Panneerselvam, D Subramaniam, M Bharathiraja
The modern scenario reveals that the world is facing energy crisis due to the dwindling sources of fossil fuels. Environment protection agencies are more concerned about the atmospheric pollution due to the burning of fossil fuels. Alternative fuel research is getting augmented because of the above reasons. Plant seed oils (vegetable oils) are cleaner, sustainable, and renewable. So, it can be the most suitable alternative fuel for compression ignition (CI) engines. This paper reviews the availability of different types of plant seed oils, several methods for production of biodiesel from vegetable oils, and its properties...
October 15, 2016: Environmental Science and Pollution Research International
Jiacheng Huang, Qianyue Liu, Bin Xue, Long Chen, Yong Wang, Shiyi Ou, Xichun Peng
In our previous study, an antihypertensive protein hydrolysate was prepared from sardine. This study aimed to investigate the composition of sardine protein hydrolysate (SPH) and it's in vivo antihypertensive effect. SPH was separated sequentially with ultrafiltration and size exclusion chromatography. Fractions with high angiotensin-I-converting enzyme (ACE) inhibitory activity were further analyzed with RP-HPLC and amino acids analysis. Then, SPH was individually oral administrated to spontaneously hypertensive rats (SHR) and normotensive wistar kyoto rats (WKY) for 4 wk...
September 28, 2016: Journal of Food Science
Brankica Kartalović, Nikolina J Novakov, Željko Mihaljev, Jelena Petrović, Nadežda Prica, Jelena Babić, Miroslav A Ćirković
The aim of this study was to determine the level of organochlorine (OC) pesticides in 57 samples of canned tuna and 31 samples of canned sardines in vegetable oil, collected from supermarkets in Serbia. OC pesticides α-HCH, β-HCH, δ-HCH, dichlorodiphenyltrichloroethane (DDT), DDE, DDD, dielderin, endosulfane I, endosulfane II, endosulan sulfate, endrin, endrin ketone, heptachlor, heptachlor epoxide, lindane, aldrin, metoxichlor, cis-chlordane and trans-chlordane were determined using a GS-MS method. The highest concentrations (µg kg(-1), arithmetic means) in canned tuna were for δ-HCH (60...
December 2016: Food Additives & Contaminants. Part B, Surveillance
Sandra Salleres, Irune González, Alai Arantzamendi, Roberto González, Sonia Maza, Arrate Jaureguibeitia, James M Hungerford, Christina A DeWitt, Ronald A Benner
The Biofish-300 HIS method is a simple, reliable, and specific enzymatic biosensor for the detection of histamine. This technology is highly specific and selective and allows quantification of histamine in fishery products (fresh/frozen and processed) in a short time frame (2-3 min). Histamine in raw tuna, raw mackerel, raw sardine, raw anchovy, boiled tuna, canned tuna in water, canned tuna in oil, canned mackerel in tomato sauce, canned pickled sardine, and canned salted anchovy was analyzed using a water-based extract...
September 2016: Journal of AOAC International
N Stern, B Rinkevich, M Goren
To deal with the difficulties of species differentiation and delimitation among the commercially important sardines from the genus Sardinella, an integrative approach was adopted, incorporating traditional taxonomy with four DNA markers (coI, cytb, 16s and nuclear rag2). Combining these methodologies has enabled a thorough re-description of three of the most common species of Sardinella of the Indo-west Pacific Ocean: white sardinella Sardinella albella, fringescale sardinella Sardinella fimbriata and the goldstripe sardinella Sardinella gibbosa, as well as a description of a new species, Gon's sardinella Sardinella goni, from the island of Boracay, Philippines...
September 12, 2016: Journal of Fish Biology
Lauren M Sassoubre, Kevan M Yamahara, Luke D Gardner, Barbara A Block, Alexandria B Boehm
Analysis of environmental DNA (eDNA) to identify macroorganisms and biodiversity has the potential to significantly augment spatial and temporal biological monitoring in aquatic ecosystems. Current monitoring methods relying on the physical identification of organisms can be time consuming, expensive, and invasive. Measuring eDNA shed from organisms provides detailed information on the presence and abundance of communities of organisms. However, little is known about eDNA shedding and decay in aquatic environments...
October 4, 2016: Environmental Science & Technology
Mohan Nadiga, V V Vaidyanathan, Thangavelu Thayumanavan
Draft genome sequencing of Aeromonas dhakensis strain F2S2-1, isolated from the skin surface of an Indian oil sardine (Sardinella longiceps), has been carried out. The draft genome was roughly 4.7 Mb in size with 61.7% G+C content. Annotation of the genome yielded 4,337 genes coding for proteins, tRNAs, and rRNAs. Annotation also revealed the presence of 52 genes linked to resistance to antibiotics/toxic compounds. Pathway analysis revealed the presence of novobiocin biosynthetic genes and genes for biosynthesis of a siderophore group on nonsynthetic peptides...
2016: Genome Announcements
Nurul Izzah Ahmad, Wan Rozita Wan Mahiyuddin, Tengku Rozaina Tengku Mohamad, Cheong Yoon Ling, Siti Fatimah Daud, Nasriyah Che Hussein, Nor Aini Abdullah, Rafiza Shaharudin, Lokman Hakim Sulaiman
BACKGROUND: Understanding different patterns of fish consumption is an important component for risk assessment of contaminants in fish. A few studies on food consumption had been conducted in Malaysia, but none of them focused specifically on fish consumption. The objectives of this study were to document the meal pattern among three major ethnics in Malaysia with respect to fish/seafood consumption, identify most frequently consumed fish and cooking method, and examine the influence of demographic factors on pattern of fish consumption among study subjects...
2016: Food & Nutrition Research
Maria Elisa Melo Branco de Araújo, Paula Renata Bueno Campos, Thiago Grando Alberto, Fabiano Jares Contesini, Patrícia de Oliveira Carvalho
The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipase and 1:5 (w/w) for Rhizopus javanicus lipase using Amberlite MB-1...
October 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Katherine E Watermeyer, Laurence Hutchings, Astrid Jarre, Lynne J Shannon
Several commercially and ecologically important species in the southern Benguela have undergone southward and eastward shifts in their distributions over previous decades, most notably the small pelagic fish sardine Sardinops sagax and anchovy Engraulis encrasicolus. Understanding these changes and their implications is essential in implementing an ecosystem approach to fisheries in the southern Benguela and attempting to appreciate the potential impacts of future environmental change. To investigate possible impacts of these shifts at an ecosystem level, distribution maps for before (1985-1991), during (1997-2000) and after (2003-2008) the shift in small pelagic fish were constructed for 14 key species from catch and survey data, and used to calculate spatial indicators including proportion east and west of Cape Agulhas, relative overlap in biomass and area, index of diversity, connectivity...
2016: PloS One
Aline Nondillo, Simone Andzeiewski, Flávio Bello Fialho, Odair Correa Bueno, Marcos Botton
Linepithema micans (Forel) (Hymenoptera: Formicidae) is the main ant species responsible for dispersal of Eurhizococcus brasiliensis (Wille) (Hemiptera: Margarodidae), a root scale that damages grapevines in southern Brazil. The effects of different formulations of toxic baits based on boric acid and hydramethylnon to control L. micans and E. brasiliensis were evaluated. Toxic baits with boric acid (1.0%) mixed in different concentrations of inverted sugar (20%, 30%, and 40%), and hydramethylnon, mixed with sardines (paste), cassava flour and peanut, brown sugar (sucrose), or sardine oil-based gel, were evaluated in a greenhouse and in the field...
August 2016: Journal of Economic Entomology
Filipa Faleiro, Marta Pimentel, Maria Rita Pegado, Regina Bispo, Ana Rita Lopes, Mário S Diniz, Rui Rosa
Small pelagic fishes are known to respond rapidly to changes in ocean climate. In this study, we evaluate the effects of future environmental warming (+2°C) during the early ontogeny of the European sardine, Sardina pilchardus. Warming reduced the survival of 30-day-old larvae by half. Length at hatching increased with temperature as expected, but no significant effect was observed on the length and growth at 30 days post-hatching. Warming did not significantly affect the thermal tolerance of sardine larvae, even though the mean lethal temperature increased by 1°C...
2016: Conservation Physiology
C R de Souza, C J Von Zuben
Sarcophagidae (Diptera) are potential vectors of several pathogens. They are also very important in forensic entomology, providing basic information on the circumstances of death. The objective of this study was to determine the synanthropic index of adult Sarcophagidae collected in Rio Claro, state of São Paulo, southeastern Brazil. Sampling occurred between September 2009 and August 2010. Traps baited with sardines, beef liver, or minced meat were set for five consecutive days per month in three distinct ecological areas representing urban, rural, and forest environments...
June 9, 2016: Neotropical Entomology
F Vida Zohoori, Anne Maguire
It is important to monitor systemic fluoride (F) intake from foods, drinks and inadvertent toothpaste ingestion in order to minimise the risk of dental fluorosis while maximising caries prevention. In the UK, an F database containing the F content of commercially available foods and drinks was compiled from 518 products analysed using an acid-diffusion method and F-ion-selective electrode. The individual products analysed ranged from <0.01 µg F/100 g for butter/margarine (miscellaneous foods group) to 1,054...
2016: Caries Research
Francesca Falco, Marco Barra, Matteo Cammarata, Angela Cuttitta, Sichao Jia, Angelo Bonanno, Salvatore Mazzola, Guoyao Wu
A comparative study was performed to identify differences in the amino acid composition of the eyes from zebrafish (Danio rerio) and sardine (Sardina pilchardus) larvae and their link to the environmental adaption of the species. Amino acids in the acidic hydrolysates of eyes from 11 zebrafish and 12 sardine were determined with the use of high-performance liquid chromatography involving precolumn derivatization with ortho-phthalaldehyde. Differences in the content of most amino acids were detected between zebrafish and sardine...
2016: SpringerPlus
F Affane, N Boukhari Benahmed Daidj, S Louala, A N Munezero, M Y Lamri-Senhadji
AIM OF THE STUDY: The effects of an obesogenic diet enriched in sardine by-products (Sardina pilchardus) on blood pressure, glycemic control and some cardiovascular risk factors, in particular pro-atherogenic were studied on an experimental model. MATERIALS AND METHODS: Male rats (n=12) weighing 200±10g were subjected to an adjustment phase of 10 days in an obesogenic diet containing 30% sheep fat. After this period, the rats were divided into two homogeneous groups...
June 2016: Annales de Cardiologie et D'angéiologie
Giuseppe Borghero, Maura Pugliatti, Francesco Marrosu, Maria Giovanna Marrosu, Maria Rita Murru, Gianluca Floris, Antonino Cannas, Patrizia Occhineri, Tea B Cau, Daniela Loi, Anna Ticca, Sebastiano Traccis, Umberto Manera, Antonio Canosa, Cristina Moglia, Andrea Calvo, Marco Barberis, Maura Brunetti, J Raphael Gibbs, Alan E Renton, Edoardo Errichiello, Magdalena Zoledziewska, Antonella Mulas, Yong Qian, Jun Din, Hannah A Pliner, Bryan J Traynor, Adriano Chiò
Recently, mutations in the TANK-binding kinase 1 (TBK1) gene were identified as a cause for amyotrophic lateral sclerosis (ALS) with or without comorbid frontotemporal dementia. We have assessed the frequency and clinical characteristics of TBK1 mutations in a cohort of ALS patients of Sardinian ancestry. Whole-exome sequencing was performed on Hiseq2000 platform (Illumina). Genome analysis Toolkit was used to align and to code variants according to Human Genome (UCSC hg19). Mutation was confirmed with Sanger sequence...
July 2016: Neurobiology of Aging
Danilo J Leao, Mario M S Junior, Geovani C Brandao, Sergio L C Ferreira
A method was established to simultaneously determine cadmium, iron and tin in canned-food samples using high-resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GF AAS). The quantification step has been performed using the primary line (228.802nm) for cadmium and the adjacent secondary lines (228.725nm and 228.668nm) for iron and tin, respectively. The selected chemical modifier was an acid solution that contained a mixture of 0.1% (w/v) Pd and 0.05% (w/v) Mg. The absorbance signals were measured based on the peak area using 3 pixels for cadmium and 5 pixels for iron and tin...
June 1, 2016: Talanta
Mariona Balfegó, Silvia Canivell, Felicia A Hanzu, Aleix Sala-Vila, Margarita Martínez-Medina, Serafín Murillo, Teresa Mur, Elena G Ruano, Francisca Linares, Nuria Porras, Silvia Valladares, Maria Fontalba, Elena Roura, Anna Novials, Cristina Hernández, Gloria Aranda, Antoni Sisó-Almirall, Gemma Rojo-Martínez, Rafael Simó, Ramon Gomis
BACKGROUND: Nutrition therapy is the cornerstone of treating diabetes mellitus. The inclusion of fish (particularly oily fish) at least two times per week is recommended by current international dietary guidelines for type 2 diabetes. In contrast to a large number of human studies examining the effects of oily fish on different cardiovascular risk factors, little research on this topic is available in patients with type 2 diabetes. The aims of this pilot study were to investigate the effects of a sardine-enriched diet on metabolic control, adiponectin, inflammatory markers, erythrocyte membrane fatty acid (EMFA) composition, and gut microbiota in drug-naïve patients with type 2 diabetes...
2016: Lipids in Health and Disease
Aitor Albaina, Mikel Aguirre, David Abad, María Santos, Andone Estonba
The potential of the 18S rRNA V9 metabarcoding approach for diet assessment was explored using MiSeq paired-end (PE; 2 × 150 bp) technology. To critically evaluate the method's performance with degraded/digested DNA, the diets of two zooplanktivorous fish species from the Bay of Biscay, European sardine (Sardina pilchardus) and European sprat (Sprattus sprattus), were analysed. The taxonomic resolution and quantitative potential of the 18S V9 metabarcoding was first assessed both in silico and with mock and field plankton samples...
March 2016: Ecology and Evolution
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