keyword
MENU ▼
Read by QxMD icon Read
search

LDLR

keyword
https://www.readbyqxmd.com/read/28817679/increased-half-life-and-enhanced-potency-of-fc-modified-human-pcsk9-monoclonal-antibodies-in-primates
#1
Yijun Shen, Hua Li, Li Zhao, Gang Li, Ben Chen, Qingsong Guo, Bei Gao, Jinsong Wu, Tong Yang, Li Jin, Yong Su
Blocking proprotein convertase subtilisin kexin type 9 (PCSK9) binding to low-density lipoprotein receptor (LDLR) can profoundly lower plasma LDL levels. Two anti-PCKS9 monoclonal antibodies (mAbs), alirocumab and evolocumab, were approved by the FDA in 2015. The recommended dose is 75 mg to 150 mg every two weeks for alirocumab and 140mg every two weeks or 420 mg once a month for evolocumab. This study attempted to improve the pharmacokinetic properties of F0016A, an IgG1 anti-PCKS9 mAb, to generate biologically superior molecules...
2017: PloS One
https://www.readbyqxmd.com/read/28812343/xanthohumol-suppresses-mylip-idol-gene-expression-and-modulates-ldlr-abundance-and-activity-in-hepg2-cells
#2
Shih-Fen Chen, Pei-Yi Chen, Hao-Jen Hsu, Ming-Jiuan Wu, Jui-Hung Yen
Xanthohumol, a prenylated flavonoid found in hops (Humulus lupulus L.), exhibits multiple biological activities such as anti-atherosclerosis and hypolipidemic activities. In this study, we aim to investigate the hypocholesterolemic effects and molecular mechanisms of xanthohumol in hepatic cells. We found that xanthohumol (10 and 20 μM) increased the amount of cell-surface low-density lipoprotein receptor (LDLR) from 100.0 ± 2.1% to 115.0±1.3% and 135.2±2.7%, and enhanced the LDL uptake activity from 100...
August 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28808193/evaluation-of-atherosclerotic-lesions-in-cholesterol-fed-mice-during-treatment-with-paclitaxel-in-lipid-nanoparticles-a-magnetic-resonance-imaging-study
#3
Aline D Lima, Ning Hua, Raul C Maranhão, James A Hamilton
Cholesterol-core nanoparticles (LDE) have been shown to be recognized by low-density lipoprotein receptors (LDLR) after administration; therefore, LDE is an ideal vehicle to deliver drug with targeting property. Paclitaxel, when incorporated into LDE, promotes atherosclerosis regression with reduced drug toxicity in rabbits through LDLR. Here, we tested whether LDE-paclitaxel could still be effective in reducing diet-induced atherosclerosis in a mouse model without LDLR. Nineteen LDLR knockout male mice were fed 1% cholesterol for 12 weeks...
January 19, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/28802576/atorvastatin-and-lovastatin-but-not-pravastatin-increased-cellular-complex-formation-between-pcsk9-and-the-ldl-receptor-in-human-hepatocyte-like-c3a-cells
#4
Quantil M Melendez, Catherine J Wooten, Dayami Lopez
Statins are the first-line treatment for hypercholesterolemic patients. Herein, the effects of three statins on complex formation between proprotein convertase subtilisin-kexin 9 (PCSK9) and the low density lipoprotein receptor (LDLR), a critical step for the PCSK9-dependent degradation of LDLR in the lysosome, were examined. Human hepatocyte-like C3A cells grown in control (containing 10% fetal bovine serum) or MITO+ (supplemented with BD™ MITO + serum extender) medium were also treated with atorvastatin (Atorv), lovastatin (Lov), or pravastatin (Prav) for 24 h...
August 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28796818/hypercholesterolemia-induced-cerebral-small-vessel-disease
#5
Peter Kraft, Michael K Schuhmann, Cornelia Garz, Solveig Jandke, Daniela Urlaub, Stine Mencl, Alma Zernecke, Hans-Jochen Heinze, Roxana O Carare, Christoph Kleinschnitz, Stefanie Schreiber
BACKGROUND: While hypercholesterolemia plays a causative role for the development of ischemic stroke in large vessels, its significance for cerebral small vessel disease (CSVD) remains unclear. We thus aimed to understand the detailed relationship between hypercholesterolemia and CSVD using the well described Ldlr-/- mouse model. METHODS: We used Ldlr-/- mice (n = 16) and wild-type (WT) mice (n = 15) at the age of 6 and 12 months. Ldlr-/- mice develop high plasma cholesterol levels following a high fat diet...
2017: PloS One
https://www.readbyqxmd.com/read/28792970/targeting-hepatic-heparin-binding-egf-like-growth-factor-hb-egf-induces-anti-hyperlipidemia-leading-to-reduction-of-angiotensin-ii-induced-aneurysm-development
#6
Seonwook Kim, Lihua Yang, Seongu Kim, Richard G Lee, Mark J Graham, Judith A Berliner, Aldons J Lusis, Lei Cai, Ryan E Temel, Debra L Rateri, Sangderk Lee
OBJECTIVE: The upregulated expression of heparin binding EGF-like growth factor (HB-EGF) in the vessel and circulation is associated with risk of cardiovascular disease. In this study, we tested the effects of HB-EGF targeting using HB-EGF-specific antisense oligonucleotide (ASO) on the development of aortic aneurysm in a mouse aneurysm model. APPROACH AND RESULTS: Low-density lipoprotein receptor (LDLR) deficient mice (male, 16 weeks of age) were injected with control and HB-EGF ASOs for 10 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28792474/the-effects-of-selective-hematopoietic-expression-of-human-il-37-on-systemic-inflammation-and-atherosclerosis-in-ldlr-deficient-mice
#7
Geerte Hoeke, P Padmini S J Khedoe, Janna A van Diepen, Karin Pike-Overzet, Britt van de Ven, Nadia Vazirpanah, Isabel Mol, Pieter S Hiemstra, Frank J T Staal, Rinke Stienstra, Mihai G Netea, Charles A Dinarello, Patrick C N Rensen, Jimmy F P Berbée
The human cytokine interleukin (IL)-37 has potent anti-inflammatory capacities, and hematopoietic cell-specific transgenic overexpression of IL-37 in mice protects against septic shock and colitis. In the present study we investigated the effect of hematopoietic expression of IL-37 on atherosclerosis development under low-grade inflammatory conditions. Low-density lipoprotein receptor (LDLr)-deficient mice were lethally irradiated and transplanted with bone marrow from IL-37-transgenic or control wild-type mice and fed a Western-type diet (WTD; 1% cholesterol) for eight weeks...
August 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28782835/identification-and-functional-analysis-of-a-potential-key-lncrna-involved-in-fat-loss-of-cancer-cachexia
#8
Huiquan Liu, Ting Zhou, Bangyan Wang, Lu Li, Dawei Ye, Shiying Yu
Cancer cachexia is a devastating, multifactorial and irreversible syndrome characterized by skeletal muscle reduction with or without fat loss. Although much attention has been focused on muscle wasting, fat loss may occur earlier and accelerate muscle wasting in cachexia. The cause of 20% of cancer related death makes it urgent to discover molecular mechanisms behind cancer cachexia. Here we applied weighted gene co-expression network analysis(WGCNA) to identify cachexia related gene modules using differentially expressed 3289 genes and 59 long non-coding RNAs based on microarray data of cachectic and non-cachectic subcutaneous adipose tissue...
August 7, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28776325/identification-and-functional-characterization-of-two-missense-mutations-in-ndrg1-associated-with-charcot-marie-tooth-disease-type-4d-cmt4d
#9
Li-Xi Li, Gong-Lu Liu, Zhi-Jun Liu, Cong Lu, Zhi-Ying Wu
Charcot-Marie-Tooth disease type 4D (CMT4D) is an autosomal recessive demyelinating form of CMT characterized by a severe distal motor and sensory neuropathy. NDRG1 is the causative gene for CMT4D. To date, only four mutations in NDRG1 - c.442C>T (p.Arg148*), c.739delC (p.His247Thrfs*74), c.538-1G>A, and duplication of exons 6-8 - have been described in CMT4D patients. Here, using targeted next-generation sequencing (NGS) examination, we identified for the first time two homozygous missense variants in NDRG1, c...
August 3, 2017: Human Mutation
https://www.readbyqxmd.com/read/28772107/family-specific-aggregation-of-lipid-gwas-variants-confers-the-susceptibility-to-familial-hypercholesterolemia-in-a-large-austrian-family
#10
Elina Nikkola, Arthur Ko, Marcus Alvarez, Rita M Cantor, Kristina Garske, Elliot Kim, Stephanie Gee, Alejandra Rodriguez, Reinhard Muxel, Niina Matikainen, Sanni Söderlund, Mahdi M Motazacker, Jan Borén, Claudia Lamina, Florian Kronenberg, Wolfgang J Schneider, Aarno Palotie, Markku Laakso, Marja-Riitta Taskinen, Päivi Pajukanta
BACKGROUND AND AIMS: Hypercholesterolemia confers susceptibility to cardiovascular disease (CVD). Both serum total cholesterol (TC) and LDL-cholesterol (LDL-C) exhibit a strong genetic component (heritability estimates 0.41-0.50). However, a large part of this heritability cannot be explained by the variants identified in recent extensive genome-wide association studies (GWAS) on lipids. Our aim was to find genetic causes leading to high LDL-C levels and ultimately CVD in a large Austrian family presenting with what appears to be autosomal dominant inheritance for familial hypercholesterolemia (FH)...
July 22, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28771708/metabolic-profiling-of-murine-plasma-reveals-eicosapentaenoic-acid-metabolites-protecting-against-endothelial-activation-and-atherosclerosis
#11
Yajin Liu, Xuan Fang, Xu Zhang, Jing Huang, Jinlong He, Liyuan Peng, Chenji Ye, Yingmei Wang, Fengxia Xue, Ding Ai, Dan Li, Yi Zhu
BACKGROUND AND PURPOSE: Atherosclerosis results from a maladaptive inflammatory response initiated by the intramural retention of LDL in susceptible areas of the arterial vasculature. ω-3 polyunsaturated fatty acids (ω-3) have protective effects in atherosclerosis; however, the molecular mechanism is still largely unknown. The present study used a metabolomic approach to reveal the atheroprotective metabolites of ω-3 and investigate the underlying mechanisms. EXPERIMENTAL APPROACH: We evaluated the development of atherosclerosis in LDL-receptor-deficient mice (LDLR(-/-) ) fed a Western-type diet (WTD) plus ω-3 and also LDLR(-/-) and fat-1 transgenic (LDLR(-/-) -fat-1(tg) ) mice fed a WTD...
August 3, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28771609/deletion-of-batf3-dependent-antigen-presenting-cells-does-not-affect-atherosclerotic-lesion-formation-in-mice
#12
Jesus Gil-Pulido, Clement Cochain, Malte A Lippert, Nicole Schneider, Elke Butt, Núria Amézaga, Alma Zernecke
Atherosclerosis is the main underlying cause for cardiovascular events such as myocardial infarction and stroke and its development might be influenced by immune cells. Dendritic cells (DCs) bridge innate and adaptive immune responses by presenting antigens to T cells and releasing a variety of cytokines. Several subsets of DCs can be discriminated that engage specific transcriptional pathways for their development. Basic leucine zipper transcription factor ATF-like 3 (Batf3) is required for the development of classical CD8α+ and CD103+ DCs...
2017: PloS One
https://www.readbyqxmd.com/read/28767725/dna-mutation-motifs-in-the-genes-associated-with-inherited-diseases
#13
Michal Růžička, Petr Kulhánek, Lenka Radová, Andrea Čechová, Naďa Špačková, Lenka Fajkusová, Kamila Réblová
Mutations in human genes can be responsible for inherited genetic disorders and cancer. Mutations can arise due to environmental factors or spontaneously. It has been shown that certain DNA sequences are more prone to mutate. These sites are termed hotspots and exhibit a higher mutation frequency than expected by chance. In contrast, DNA sequences with lower mutation frequencies than expected by chance are termed coldspots. Mutation hotspots are usually derived from a mutation spectrum, which reflects particular population where an effect of a common ancestor plays a role...
2017: PloS One
https://www.readbyqxmd.com/read/28766168/pre-and-post-prandial-expression-of-genes-involved-in-lipid-metabolism-at-the-end-of-the-overfeeding-period-of-mule-ducks
#14
Tavernier Annabelle, Ricaud Karine, Bernadet Marie-Dominique, Gontier Karine, Davail Stéphane
In palmipeds, overfeeding leads to hepatic steatosis, also called "foie gras" which is the result of many metabolic mechanisms. In order to understand these mechanisms, we decided to measure the expression of genes implicated in lipid metabolism during 12 hours (h) following the last meal of the overfeeding period. We have shown that there is a precocious expression (within 2 h) of fatty acid synthase and acyl CoA synthetase long-chain 1 in liver and muscle of mule ducks in addition with a later peak. Furthermore, di-acyl glycerol acyl transferase presents the highest induction of expression in liver and it is overexpressed quite a long time, positioning this enzyme as a key factor in hepatic steatosis...
August 1, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28764195/whole-exomes-sequencing-delineates-gene-variants-profile-in-a-young-saudi-male-with-familial-hypercholesterolemia-case-report
#15
Edem Nuglozeh
Familial hypercholesterolemia is an autosomal dominant genetic disease characterized by earlier elevated Low-Density Lipoprotein (LDL) cholesterol levels and increased risk for premature Myocardial Infarction (MI). Albeit the diagnosis of some medical Familial Hypercholesterolemia (FH) cases are due to mutations in PCSK9, APOB, or LDLR, detection of mutation rate and profiles relies heavily on different gene pools and ethnicity. We ran exome sequencing on blood genomic DNA (gDNA) from a 26-year-old Saudi patient on Ion Proton Platform (Ion Torrent, Guilford, Connecticut, USA) as part of a pilot study preluding the establishment of the Saudi Human Genome project...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28762182/diet-induced-hypercholesterolemia-alters-liver-glycosaminoglycans-and-associated-lipoprotein-receptors-in-rats
#16
Gangappa Kiran, Ummiti J S Prasada Rao, Paramahans V Salimath, Nandini D Chilkunda
Glycosaminoglycans (GAGs) play an important role in lipoprotein metabolism. In liver, it facilitates the uptake of remnants through receptor-independent endocytosis. However, changes in liver GAGs during diet-induced hypercholesterolemia with normal levels of fat feeding are unknown. Present paper highlights the effect of diet-induced hypercholesterolemia with normal levels (5%) of fat on liver GAGs and other associated lipoprotein receptors. Hypercholesterolemia was induced in rats by feeding diet supplemented with 0...
July 31, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28759786/high-purity-tocotrienols-attenuate-atherosclerotic-lesion-formation-in-apoe-ko-mice
#17
Akira Shibata, Teiko Kobayashi, Akira Asai, Takahiro Eitsuka, Shinichi Oikawa, Teruo Miyazawa, Kiyotaka Nakagawa
Previous studies have demonstrated that tocotrienol (T3) has antiatherogenic effects. However, the T3 preparations used in those studies contained considerable amounts of tocopherol (Toc), which might affect the biological activity of T3. There is little information on the effect of highly purified T3 on atherosclerosis formation. This study investigated the effect of high-purity T3 on atherosclerotic lesion formation and the underlying mechanisms. Male apolipoprotein E knockout (apoE-KO) mice were fed a cholesterol-containing diet either alone or supplemented with T3 concentrate (Toc-free T3) or with α-Toc for 12 weeks...
June 24, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28759039/elevated-tumor-ldlr-expression-accelerates-ldl-cholesterol-mediated-breast-cancer-growth-in-mouse-models-of-hyperlipidemia
#18
E J Gallagher, Z Zelenko, B A Neel, I M Antoniou, L Rajan, N Kase, D LeRoith
Obesity is associated with an increase in cancer-specific mortality in women with breast cancer. Elevated cholesterol, particularly low-density lipoprotein cholesterol (LDL-C), is frequently seen in obese women. Here, we aimed to determine the importance of elevated circulating LDL, and LDL receptor (LDLR) expression in tumor cells, on the growth of breast cancer using mouse models of hyperlipidemia. We describe two novel immunodeficient mouse models of hyperlipidemia (Rag1(-/)(-)/LDLR(-/)(-) and Rag1(-/)(-)/ApoE (apolipoprotein E)(-/)(-) mice) in addition to established immunocompetent LDLR(-/)(-) and ApoE(-/)(-) mice...
July 31, 2017: Oncogene
https://www.readbyqxmd.com/read/28758667/reciprocal-upregulation-of-scavenger-receptors-complicates-interpretation-of-nanoparticle-uptake-in-non-phagocytic-cells
#19
Kanlaya Prapainop, Rong Miao, Christoffer Åberg, Anna Salvati, Kenneth A Dawson
Nanoparticles have great potential as drug delivery vehicles or as imaging agents for treatment and diagnosis of various diseases. It is therefore crucial to understand how nanoparticles are taken up by cells, both phagocytic and non-phagocytic. Small interference RNA has previously been used to isolate the effect of particular receptors in nanoparticle uptake by silencing their expression. Here we show that, when it comes to receptors with overlapping function, interpretation of such data has to be done with caution...
July 31, 2017: Nanoscale
https://www.readbyqxmd.com/read/28753643/pharmacogenetic-meta-analysis-of-baseline-risk-factors-pharmacodynamic-efficacy-and-tolerability-endpoints-from-two-large-global-cardiovascular-outcomes-trials-for-darapladib
#20
Astrid Yeo, Li Li, Liling Warren, Jennifer Aponte, Dana Fraser, Karen King, Kelley Johansson, Allison Barnes, Colin MacPhee, Richard Davies, Stephanie Chissoe, Elizabeth Tarka, Michelle L O'Donoghue, Harvey D White, Lars Wallentin, Dawn Waterworth
Darapladib, a lipoprotein-associated phospholipase A2 (Lp-PLA2) inhibitor, failed to demonstrate efficacy for the primary endpoints in two large phase III cardiovascular outcomes trials, one in stable coronary heart disease patients (STABILITY) and one in acute coronary syndrome (SOLID-TIMI 52). No major safety signals were observed but tolerability issues of diarrhea and odor were common (up to 13%). We hypothesized that genetic variants associated with Lp-PLA2 activity may influence efficacy and tolerability and therefore performed a comprehensive pharmacogenetic analysis of both trials...
2017: PloS One
keyword
keyword
42177
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"