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https://www.readbyqxmd.com/read/29907992/surface-chemistry-substrate-and-topography-guide-the-behavior-of-human-articular-chondrocytes-cultured-in-vitro
#1
Natasja Leth Bergholt, Morten Foss, Anwer Saeed, Nikolaj Gadegaard, Helle Lysdahl, Martin Lind, Casper Bindzus Foldager
Understanding the behavior of chondrocytes in contact with artificial culture surfaces is becoming increasingly important in attaining appropriate ex vivo culture conditions of chondrocytes in cartilage regeneration. Chondrocyte transplantation-based cartilage repair requires efficiently expanded chondrocytes and the culture surface plays an important role in guiding the behavior of the cell. Micro- and nanoengineered surfaces make it possible to modulate cell behavior. We hypothesized that the combined influence of topography, substrate, and surface chemistry may affect the chondrocyte culturing in terms of proliferation and phenotypic means...
June 16, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29875037/the-impact-of-mir200-on-extracellular-matrix-topography-guided-epithelial-to-mesenchymal-transition-of-prostate-cancer-cells
#2
Tuğba Özdemir
OBJECTIVE: Epithelial to Mesenchymal Transition (EMT) is an important phenomenon that is recently been recognized to play roles on prostate cancer metastasis through both epigenetic and biochemical signaling pathways. Using tissue engineering tools, we recreated a metastatic tumor niche to study the role of mir200 (a small RNA proven to reverse EMT processes) on extracellular matrix (ECM) fiber diameter guided prostate cancer cell EMT. MATERIAL AND METHODS: LNCaP cells were cultured on fibrous scaffolds for 48 hours...
May 31, 2018: Turkish Journal of Urology
https://www.readbyqxmd.com/read/29787391/comparing-wavefront-optimized-wavefront-guided-and-topography-guided-laser-vision-correction-clinical-outcomes-using-an-objective-decision-tree
#3
Karl Stonecipher, Joseph Parrish, Megan Stonecipher
PURPOSE OF REVIEW: This review is intended to update and educate the reader on the currently available options for laser vision correction, more specifically, laser-assisted in-situ keratomileusis (LASIK). In addition, some related clinical outcomes data from over 1000 cases performed over a 1-year are presented to highlight some differences between the various treatment profiles currently available including the rapidity of visual recovery. The cases in question were performed on the basis of a decision tree to segregate patients on the basis of anatomical, topographic and aberrometry findings; the decision tree was formulated based on the data available in some of the reviewed articles...
May 18, 2018: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/29754993/topography-guided-lasik-versus-small-incision-lenticule-extraction-long-term-refractive-and-quality-of-vision-outcomes
#4
Anastasios John Kanellopoulos
No abstract text is available yet for this article.
May 10, 2018: Ophthalmology
https://www.readbyqxmd.com/read/29675388/consistent-comparison-of-angle-kappa-adjustment-between-oculyzer-and-topolyzer-vario-topography-guided-lasik-for-myopia-by-ex500-excimer-laser
#5
Ming-Shen Sun, Li Zhang, Ning Guo, Yan-Zheng Song, Feng-Ju Zhang
AIM: To evaluate and compare the uniformity of angle Kappa adjustment between Oculyzer and Topolyzer Vario topography guided ablation of laser in situ keratomileusis (LASIK) by EX500 excimer laser for myopia. METHODS: Totally 145 cases (290 consecutive eyes )with myopia received LASIK with a target of emmetropia. The ablation for 86 cases (172 eyes) was guided manually based on Oculyzer topography (study group), while the ablation for 59 cases (118 eyes) was guided automatically by Topolyzer Vario topography (control group)...
2018: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29610714/visual-and-refractive-outcomes-of-topography-guided-laser-assisted-in-situ-keratomileusis-in-virgin-eyes
#6
Sharif Hashmani, Nauman Hashmani, Husna Haroon, Yusra Hashmi
Introduction We wanted to assess the efficacy, predictability, and stability of topography-guided, laser-assisted in situ keratomileusis (TGL) on normal untreated eyes with a preoperative best corrected visual acuity (BCVA) of 20/20. Methods This was a retrospective, non-randomized, and single arm study evaluating the outcomes of TGL in eyes with a preoperative BCVA of 20/20. We included 50 eyes of 50 patients who presented to the Hashmanis Hospital, Pakistan and were followed for six months postoperatively...
January 31, 2018: Curēus
https://www.readbyqxmd.com/read/29610024/topography-guided-transepithelial-photorefractive-keratectomy-to-correct-irregular-refractive-errors-after-radial-keratotomy
#7
Mohammad Ghoreishi, Alireza Peyman, Nima Koosha, Khodayar Golabchi, Mohsen Pourazizi
PURPOSE: To evaluate the efficacy and safety of topography-guided transepithelial photorefractive keratectomy (PRK) in a group of patients with post-radial keratotomy (RK) showing irregular corneas. SETTING: Feiz University Hospital, Isfahan, Iran. DESIGN: Prospective case series. METHODS: Consecutive patients who had irregular astigmatism after RK with stable refraction and topography for at least 1 year were recruited...
March 2018: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/29457860/controlling-the-outgrowth-and-functions-of-neural-stem-cells-the-effect-of-surface-topography
#8
REVIEW
Chara Simitzi, Kanelina Karali, Anthi Ranella, Emmanuel Stratakis
Neural stem cells (NSCs) are self-renewing cells that generate the major cell types of the central nervous system, namely neurons, astrocytes and oligodendrocytes, during embryonic development and in the adult brain. NSCs reside in a complex niche where they are exposed to a plethora of signals, including both soluble and physical signals such as compressive and shear stresses, but also discontinuities and differences in morphology of the extracellular environment, termed as topographical features. Different approaches that incorporate artificial micro- and nano-scale surface topographical features have been developed aiming to recapitulate the in vivo NSC niche discontinuities and features, particularly for in vitro studies...
February 19, 2018: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29425391/incidence-and-outcomes-of-optical-zone-enlargement-and-recentration-after-previous-myopic-lasik-by-topography-guided-custom-ablation
#9
Dan Z Reinstein, Timothy J Archer, Glenn I Carp, Alastair J Stuart, Elizabeth L Rowe, Andrew Nesbit, Tara Moore
PURPOSE: To report the incidence, visual and refractive outcomes, optical zone enlargement, and recentration using topography-guided CRS-Master TOSCA II software with the MEL 80 excimer laser (Carl Zeiss Meditec AG, Jena, Germany) after primary myopic laser refractive surgery. METHODS: Retrospective analysis of 73 eyes (40 patients) with complaints of night vision disturbances due to either a decentration or small optical zone following a primary myopic laser refractive surgery procedure using the MEL 80 laser...
February 1, 2018: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/29375990/5-year-follow-up-of-combined-non-topography-guided-photorefractive-keratectomy-and-corneal-collagen-cross-linking-for-keratoconus
#10
Abdulrahman Mohammed Al-Amri
AIM: To evaluate the visual outcomes of simultaneous non-topography guided photorefractive keratectomy (PRK) and corneal collagen cross-linking (CXL) in eyes with keratoconus 5y after the procedure. METHODS: Prospective, interventional, non-randomized, and non-controlled case series design was used. Sixty eyes of 30 patients (16 males and 14 females; age: 21-41y) with mild, non-progressive (stages 1-2) keratoconus were enrolled. Refraction, uncorrected distance visual acuity (UDVA) and corrected distance visual acuity (CDVA), flat and steep keratometry readings, and adverse events were evaluated preoperatively and postoperatively...
2018: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29301768/topography-guided-identification-of-leakage-point-in-central-serous-chorioretinopathy-a-base-for-fluorescein-angiography-free-focal-laser-photocoagulation
#11
Dmitrii S Maltsev, Alexei N Kulikov, Jay Chhablani
PURPOSE: To identify optical coherence tomography (OCT) findings associated with the leakage points in patients with central serous chorioretinopathy (CSC) to provide fluorescein angiography (FA)-free focal laser photocoagulation (FLP) of the leakage point. METHODS: A retrospective study included 48 patients with CSC (48 eyes). Colocalisation of leakage points with pigment epithelial detachments (PEDs) and with areas of photoreceptor outer segments (PROS) layer thinning was evaluated with OCT...
January 4, 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29215394/visual-outcomes-and-aberrometric-changes-with-topography-guided-photorefractive-keratectomy-treatment-of-irregular-astigmatism-after-penetrating-keratoplasty
#12
Francisco Bandeira E Silva, Rossen Mihaylov Hazarbassanov, Eduardo Martines, José Luis Güell, Ana L Hofling-Lima
PURPOSE: To evaluate the visual acuity and keratometric and aberrometric changes in patients with corneal transplants (PKP), who underwent topography-guided photorefractive keratectomy (TG-PRK) with mitomycin C (MMC). METHODS: In this case study, 15 patients with spherical equivalents ranging from -11.00 to -0.25 diopters (D) who underwent penetrating corneal transplantation and had irregular astigmatism ranging from -7.5 to -2.0 D underwent TG-PRK with MMC. Corneal topography and wavefront of all patients were measured preoperatively and 12 months postoperatively...
March 2018: Cornea
https://www.readbyqxmd.com/read/29104650/predictive-factors-of-pancreatic-necrosectomy-following-percutaneous-catheter-drainage-as-a-primary-treatment-of-patients-with-infected-necrotizing-pancreatitis
#13
Xi Cao, Feng Cao, Ang Li, Xiang Gao, Xiao-Hui Wang, Dian-Gang Liu, Yu Fang, Dong-Hai Guo, Fei Li
Pancreatic necrosectomy (PN) following percutaneous catheter drainage (PCD) is an effective method of treating patients with necrotizing pancreatitis, however, the predictive factors for PN after PCD have not yet been identified. A total of 74 patients with suspected infected necrotizing pancreatitis (INP) and peripancreatic fluid collection were enrolled in the current study between October 2010 and October 2015. These patients received ultrasound or computer topography guided PCD followed by PN. Patients were divided into two groups: i) A PCD-alone group (n=32) and ii) a PCD+necrosectomy group (n=42)...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28991336/four-stage-procedure-for-keratoconus-icrs-implantation-corneal-cross-linking-toric-phakic-intraocular-lens-implantation-and-topography-guided-photorefractive-keratectomy
#14
Efekan Coskunseven, Daya Papalkar Sharma, Michael A Grentzelos, Onurcan Sahin, George D Kymionis, Ioannis Pallikaris
PURPOSE: To evaluate a four-stage combined treatment for keratoconus including intrastromal corneal ring segment (ICRS) implantation followed by corneal cross-linking (CXL), toric phakic intraocular lens (IOL) implantation, and topography-guided photorefractive keratectomy (TG-PRK). METHODS: In this retrospective interventional case series, 11 eyes of 7 patients with progressive keratoconus were treated with a four-stage procedure including the following: Keraring ICRS (Mediphacos Ltda, Belo Horizonte, Brazil) implantation followed by CXL, phakic IOL implantation, and TG-PRK (minimum 6 months between each stage)...
October 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28914632/stepwise-guided-photorefractive-keratectomy-in-treatment-of-irregular-astigmatism-after-penetrating-keratoplasty-and-deep-anterior-lamellar-keratoplasty
#15
Nir Sorkin, Adi Einan-Lifshitz, Sagi Abelson, Tanguy Boutin, Mahmood Showail, Armand Borovik, Zach Ashkenazy, Clara C Chan, David S Rootman
PURPOSE: To report the outcome of stepwise ablation using topography-guided photorefractive keratectomy to treat irregular astigmatism after either penetrating keratoplasty (PKP) or deep anterior lamellar keratoplasty (DALK). METHODS: This is a retrospective, interventional analysis including patients with irregular astigmatism after either PKP or DALK, who underwent topography-guided photorefractive keratectomy. The entire cohort was analyzed, as well as the PKP and DALK groups separately...
November 2017: Cornea
https://www.readbyqxmd.com/read/28902914/oxidative-stress-induces-dysregulated-autophagy-in-corneal-epithelium-of-keratoconus-patients
#16
Rohit Shetty, Anupam Sharma, Natasha Pahuja, Priyanka Chevour, Neeraja Padmajan, Kamesh Dhamodaran, Chaitra Jayadev, Rudy M M A Nuijts, Arkasubhra Ghosh, Jeyabalan Nallathambi
Oxidative stress is one of the key factors that contributes to the pathogenesis of keratoconus (KC). Macroautophagy is a vital cellular mechanism that is activated in response to oxidative stress. The aim of this study was to understand the role of the autophagic lysosomal pathway in the oxidative damage of KC corneal epithelium and the human corneal epithelial cell line (HCE).The corneal epithelium was collected from 78 KC patients undergoing corneal cross-linking or topography guided photorefractive keratectomy...
2017: PloS One
https://www.readbyqxmd.com/read/28880340/topography-guided-photorefractive-keratectomy-in-the-treatment-of-corneal-scarring
#17
Nir Sorkin, Adi Einan-Lifshitz, Tanguy Boutin, Mahmood Showail, Armand Borovik, Clara C Chan, David S Rootman
PURPOSE: To report the outcome of topography-guided photorefractive keratectomy (TG-PRK) in the treatment of patients with corneal scarring. METHODS: A retrospective, interventional case series including 6 eyes of 6 patients with corneal scarring and irregular astigmatism who underwent TG-PRK. The etiologies for scarring were: infectious corneal ulcers, foreign body trauma, LASIK flap buttonhole, and lamellar keratoplasties performed to correct corneal perforation secondary to corneal melting...
September 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28757164/surface-topography-guides-morphology-and-spatial-patterning-of-induced-pluripotent-stem-cell-colonies
#18
Giulio Abagnale, Antonio Sechi, Michael Steger, Qihui Zhou, Chao-Chung Kuo, Gülcan Aydin, Carmen Schalla, Gerhard Müller-Newen, Martin Zenke, Ivan G Costa, Patrick van Rijn, Arnold Gillner, Wolfgang Wagner
The relevance of topographic cues for commitment of induced pluripotent stem cells (iPSCs) is largely unknown. In this study, we demonstrate that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, and direct the polarity of cell division. Elongation of iPSC colonies impacts also on their intrinsic molecular patterning, which seems to be orchestrated from the rim of the colonies. BMP4-induced differentiation is enhanced in elongated colonies, and the submicron grooves impact on the spatial modulation of YAP activity upon induction with this morphogen...
August 8, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28724823/topography-guided-neutralization-technique-for-the-management-of-flap-complication-in-laser-in-situ-keratomileusis
#19
Rohit Shetty, Rushad Shroff, Tushar Grover, T Roshan, Chaitra Jayadev
A 29-year-old male was referred following a flap loss after the creation of a thin, irregular flap with a visual acuity of logMAR 0.1 with -2.0 DS-2.75 DC × 175°. Corneal topography and anterior segment optical coherence tomography revealed an irregular corneal curvature and epithelial profile. Phototherapeutic keratectomy (PTK) followed by Topography - Guided Custom Ablation Treatment (TCAT), as a modification of the topographic neutralization technique protocol, was planned to regularize the corneal surface and treat the residual refractive error...
July 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28706442/topography-modified-refraction-adjustment-of-treated-cylinder-amount-and-axis-to-the-topography-versus-standard-clinical-refraction-in-myopic-topography-guided-lasik
#20
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