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https://www.readbyqxmd.com/read/28747195/oleic-acid-induces-down-regulation-of-the-granulosa-cell-identity-marker-foxl2-and-up-regulation-of-the-sertoli-cell-marker-sox9-in-bovine-granulosa-cells
#1
Vengala Rao Yenuganti, Jens Vanselow
During negative energy balance, the concentration of different fatty acids, especially of oleic acid (OA) increases in the follicular fluid of cattle. Previously, we showed that OA induced morphological, physiological and molecular changes in cultured bovine granulosa cells. In our present study we analyzed effects of OA on the expression of markers for granulosa and Sertoli cell identity, FOXL2 and SOX9, respectively, in addition to effects on the FOXL2 regulated genes ESR2, FST, PTGS2 and PPARG. The results showed that OA down-regulated FOXL2, ESR2, FST and PPARG but up-regulated PTGS2 and SOX9...
July 26, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28738699/caspase-3-gene-expression-in-human-luteinized-granulosa-cells-is-inversely-correlated-with-the-number-of-oocytes-retrieved-after-controlled-ovarian-stimulation
#2
Verônica N Lobach, Maíra Casalechi, Cynthia Dela Cruz, Maria T Pereira, Helen L Del Puerto, Fernando M Reis
Granulosa cells control oocyte maturation through paracrine signalling and changes to the microenvironment around the oocyte. Apoptosis occurs as a physiological mechanism of granulosa cell renewal, but how it relates with the ovarian response to induced ovulation is still unclear. Therefore, this study evaluated apoptosis-related gene expression levels in granulosa cells of patients undergoing controlled ovarian stimulation. We enrolled prospectively 59 consecutive IVF patients referred to a tertiary academic hospital for couple infertility treatment...
July 25, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28735105/inherent-inferior-quality-of-follicular-fluid-in-repeat-breeder-heifers-as-evidenced-by-low-rates-of-in%C3%A2-vitro-production-of-bovine-embryos
#3
M Kafi, M Azari, O Chashnigir, S Gharibzadeh, Z Aghabozorgi, A Asaadi, M R Divar
The aims of the present study were to determine the effect of follicular fluid obtained from the ovulatory follicle of repeat breeder heifers on in vitro oocyte maturation (Experiment 1), fertilization (Experiment 2) and production of bovine embryos (Experiment 3). Holstein virgin heifers (VH, n = 5) with normal fertility or repeat breeder syndrome (RBH, n = 5) were used in the present study. Follicular fluid of VH and RBH was aspirated from ovulatory follicles and used as maturation medium. Bovine oocytes were aspirated from follicles of slaughterhouse ovaries and randomly allocated in three groups; in Group 1, oocytes cultured in TCM-199 supplemented with 10% heat-treated fetal calf serum and hormones (5 IU/mL hCG plus 0...
July 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/28729523/malignancy-rates-of-thyroid-cytology-cyst-fluid-benign-or-non-diagnostic
#4
Güven Güney, İbrahim Tayfun Şahiner
BACKGROUND We sought to investigate subgroup distribution using Bethesda classification and risks for malignancy. We also compared the malignancy risk of cases that were denoted as non-diagnostic due to cystic contents, with cases that were denoted as non-diagnostic due to presence of other features. MATERIAL AND METHODS The study included pathology test results of 1,440 thyroid nodule samples diagnosed using Bethesda classification. Results of 305 thyroidectomy excision specimens from these patients were also compared with cytology results to determine the frequency of malignancy...
July 21, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28729464/mir-15a-5p-levels-correlate-with-poor-ovarian-response-in-human-follicular-fluid
#5
Kaiyue Zhang, Wanxia Zhong, Wei-Ping Li, Zijiang Chen, Cong Zhang
Poor ovarian response is a significant problem encountered during in vitro fertilization and embryo transfer procedures. Many infertile women may suffer from poor ovarian response and its incidence tends to be increasing in young patients nowadays. It is a major cause of maternal infertility because it is associated with low pregnancy and live birth rates. However, the cause of poor ovarian response is not clear. In this study, we extracted microRNAs from human follicular fluid and performed miRNA sequencing to investigate a potential posttranscriptional mechanism underlying poor ovarian response...
July 20, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28722670/follicular-metabolic-changes-and-effects-on-oocyte-quality-in-polycystic-ovary-syndrome-patients
#6
Yan Zhang, Lingyan Liu, Tai-Lang Yin, Jing Yang, Cheng-Liang Xiong
Polycystic ovary syndrome (PCOS) is a common complex and heterogeneous disorder, affecting up to 10% women at reproductive age. It causes three fourth of the ovulatory infertility and PCOS patients often give poor IVF quality. Although some metabolic profiles have been investigated in PCOS patient sera and urine, the follicular fluid, providing fruitful biochemical information about oocyte environment during development has been ignored. In this work, based on NMR metabolomics approach, metabolic profile of follicular fluid of PCOS patients has been explored and compared with healthy controls...
July 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28708538/glycosidases-in-porcine-follicular-fluid-and-their-effect-on-zona-pellucida-awn-1-spermadhesin-interaction
#7
Tomáš Dráb, Štěpán Ren, Pavla Maňásková-Postlerová, Marie Tichá, Věra Jonáková, Jiří Liberda
Oligosaccharide moieties on the surface of the oocyte belong to the key molecules that direct the course of fertilization and are subjected to changes during oocyte maturation in the follicle. In our study, we focused on the activities of five glycosidases in the fluids from porcine secondary and preovulatory follicles (α-l-fucosidase, α-d-galactosidase, β-d-galactosidase, β-D-N-acetylhexosaminidase, and α-d-mannosidase). All of them were detected active at neutral and acidic pH. However, changes in their activities associated with follicle development were observed only in the case of α-d-mannosidase, which was increased (P < 0...
June 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/28707233/inflammatory-follicular-cysts-associated-to-necrotic-primary-teeth
#8
N P Marques, N C T Marques, V T Sakai, J A C Hanemann
BACKGROUND: Cysts are pathological cavities lined by epithelium and supported by connective tissue, containing fluid or semi-fluid substances and presenting developmental or inflammatory origins. CASE REPORT: This study aimed to evaluate a case series of inflammatory follicular cysts in children to clarify their nomenclature, diagnosis, clinical implications and treatment outcomes. Prevalence, aetio-pathogenesis, clinical, radiographic and microscopic findings, treatments and sequelae of this condition were assessed by using the records of patients treated between 2000 and 2015...
July 13, 2017: European Archives of Paediatric Dentistry: Official Journal of the European Academy of Paediatric Dentistry
https://www.readbyqxmd.com/read/28701210/follicular-fluid-redox-involvement-for-ovarian-follicle-growth
#9
REVIEW
Cláudia Freitas, Ana Catarina Neto, Liliana Matos, Elisabete Silva, Ângela Ribeiro, João Luís Silva-Carvalho, Henrique Almeida
As the human ovarian follicle enlarges in the course of a regular cycle or following controlled ovarian stimulation, the changes in its structure reveal the oocyte environment composed of cumulus oophorus cells and the follicular fluid (FF).In contrast to the dynamic nature of cells, the fluid compartment appears as a reservoir rich in biomolecules. In some aspects, it is similar to the plasma, but it also exhibits differences that likely relate to its specific localization around the oocyte. The chemical composition indicates that the follicular fluid is able to detect and buffer excessive amounts of reactive oxygen species, employing a variety of antioxidants, some of them components of the intracellular milieu...
July 12, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28696790/competitive-inhibition-of-amino-acid-transport-in-human-preovulatory-ovarian-follicles
#10
Maciej Jóźwik, Marcin Jóźwik, Anna Justyna Milewska, Frederick C Battaglia, Michał Jóźwik
To date we have yet to examine whether amino acid (AA) transport in human ovarian follicles is affected by competitive inhibition. In contrast, transplacental transfer of AAs in late-gestation sheep is characterized by reciprocal competition. This phenomenon has been described by algebraic equations of umbilical uptake of AAs based on maternal arterial concentrations. In the present translational study at a university teaching hospital, we verified whether these equations apply to the transport of AAs from blood to follicular fluid (FF) in human preovulatory follicles...
July 11, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28691325/cellular-and-extracellular-vesicular-origins-of-mirnas-within-the-bovine-ovarian-follicle
#11
G M Andrade, F V Meirelles, F Perecin, J C da Silveira
The ovarian follicle components must provide an ideal environment to ensure the success of reproductive processes, and communication between follicular cells is crucial to support proper oocyte growth. Recently, it has been demonstrated that the presence of extracellular vesicles (EVs) carrying microRNAs (miRNAs) in follicular fluid represents an important autocrine and paracrine communication mechanism inside the ovarian follicle. In this study, we tested the hypothesis that the miRNA content of EVs isolated from ovarian follicular (granulosa and cumulus-oocyte complexes) cell-conditioned culture media is dependent upon cell type...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691312/paraoxonase-1-pon1-activity-in-serum-follicular-fluid-and-seminal-plasma-of-sheep
#12
J Pradieé, F T de Campos, Jaa Rincon, L Collares, K Goularte, Pas Silveira, Lmc Pegoraro, A Schneider
This work aimed to describe the activity of paraoxonase 1 (PON1) in serum, follicular fluid and seminal plasma of sheep. Average serum PON1 activity was 286.8 ± 96.2 U/ml in females and 237.6 ± 18.9 U/ml in males. There was a positive correlation between PON1 activity in serum and follicular fluid in females, being twice higher in serum than in follicular fluid (148.8 ± 15.7 U/ml). PON1 activity in males' serum was 10-fold higher than in seminal plasma (21.18 ± 14.2 U/ml), and there was no correlation between PON1 activity in both compartments...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28688923/paraoxonase-activities-in-human-follicular-fluid-role-in-follicular-maturation
#13
Susana Meijide, Irantzu Pérez-Ruiz, M Luisa Hernández, Rosaura Navarro, Marcos Ferrando, Zaloa Larreategui, José-Ignacio Ruiz-Sanz, M Begoña Ruiz-Larrea
The paraoxonases (PONs) are antioxidant enzymes associated with beneficial effects against several diseases and some exposures. Little is known, however, about the role of PONs in human reproduction. This work was conducted to investigate whether any association existed between the activities of the PON enzymes (1, 2, and 3) with the follicular size and fertility parameters in assisted reproduction. The study included 100 subfertile women (patients) and 55 proven fertile women (oocyte donors), all undergoing an ovarian stimulation cycle...
June 22, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28684292/the-polymorphic-insertion-of-the-luteinizing-hormone-receptor-inslq-show-a-negative-association-to-lhr-gene-expression-and-to-the-follicular-fluid-hormonal-profile-in-human-small-antral-follicles
#14
T Borgbo, J Chrudimska, M Macek, J V Jeppesen, J A Bøtkjær, S G Kristensen, K T Macklon, E Ernst, L L Hansen, C Yding Andersen
The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Müllerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to -/insLQ and the -/- genotypes...
July 3, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28684220/correlation-of-serum-anti-mullerian-hormone-to-follicular-follicle-stimulating-hormone-and-implantation-potential-of-the-ensuing-embryos
#15
Maw-Sheng Lee, Shu-Ling Tzeng, Shun-Fa Yang, Yi-Ping Lin, En-Hui Cheng, Chun-Chia Huang, Yu-Shih Yang, Tsung-Hsien Lee
Serum anti-Mullerian hormone (AMH) is a predictor of the pregnancy outcome in assisted reproductive technology (ART) cycles, particularly for older women. This study attempts to elucidate the effect of serum AMH on follicular hormone profiles and implantation outcome of the ensuing embryos in ART cycles. A total of 412 patients undergoing ART cycles at a private infertility center were included and 780 follicular fluid samples were collected. Levels of follicular hormones, including FSH, LH, inhibin B, AMH, estradiol, progesterone, and androstenedione were measured...
July 3, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28683759/intravenous-neutralization-of-vascular-endothelial-growth-factor-reduces-vascular-function-permeability-of-the-ovary-and-prevents-development-of-ohss-like-symptoms-in-rhesus-monkeys
#16
C V Bishop, D M Lee, O D Slayden, X Li
BACKGROUND: Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with elevated serum VEGFA following chorionic gonadotropin (hCG) exposure in controlled ovarian stimulation (COS) cycles in women. In this study, we tested the effect of intravenous VEGFA neutralization on OHSS-like symptoms and vascular function in rhesus macaques during COS cycles. METHODS: Monkeys (n = 8) were treated with 3 COS protocols and assigned randomly to groups as follows: 1) COS alone (Control, n = 5); 2) COS + VEGF mAb Avastin 19 ± 5 h before hCG (Avastin pre-hCG; n = 6); 3) COS + Avastin 3-4 days post-hCG (Avastin post-hCG; n = 4); 4) COS + Simulated Early Pregnancy (SEP n = 3); or 5) COS + SEP + Avastin (SEP + Avastin n = 3)...
July 6, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28679464/linoleic-la-and-linolenic-ala-acid-concentrations-in-follicular-fluid-of-prepubertal-goats-and-their-effect-on-oocyte-in-vitro-maturation-and-embryo-development
#17
Montserrat Roura, María G Catalá, Sandra Soto-Heras, Sondes Hammami, Dolors Izquierdo, Ali Fouladi-Nashta, Maria-Teresa Paramio
In this study we assessed the concentration of linoleic acid (LA) and linolenic acid (ALA) in follicular fluid of prepubertal goats according to follicle size (<3mm or ≥3mm) by gas chromatography and tested the addition of different LA and ALA (LA:ALA) concentration ratios (50:50, 100:50 and 200:50µM) to the IVM medium on embryo development, mitochondrial activity, ATP concentration and relative gene expression (RPL19, ribosomal protein L19; SLC2A1, facilitated glucose transporter 1; ATF4, activating transcription factor 4; GPX1, glutathione peroxidase 1; HSPA5, heat-shock protein family A 70 kDa; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; DNMT1, DNA methyltransferase 1; GCLC, glutamate-cysteine ligase catalytic subunit; SOD1, superoxide dismutase 1)...
July 6, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28670428/evaluation-of-relationship-between-body-mass-index-with-vitamin-d-receptor-gene-expression-and-vitamin-d-levels-of-follicular-fluid-in-overweight-patients-with-polycystic-ovary-syndrome
#18
Esmat Aghadavod, Hakimeh Mollaei, Mohammad Nouri, Hamed Hamishehkar
BACKGROUND: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder associated with reproductive disorders and metabolic dysfunctions including insulin resistance. The roles of vitamin D in the regulation of metabolic modulations specifically involving insulin and reproduction processing are introduced. In addition, obesity appears to be closely associated with severity of PCOS. The present study is to evaluate the effect of body mass index (BMI) on vitamin D levels in follicular fluid and vitamin D receptor (VDR) expression levels in granulos cells...
July 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28669481/impact-of-the-opioid-system-on%C3%A2-the%C3%A2-reproductive-axis
#19
REVIEW
Bettina Böttcher, Beata Seeber, Gerhard Leyendecker, Ludwig Wildt
Endogenous opioids, first described more than 40 years ago, have long been recognized for their main role as important neuromodulators within the central nervous system. More recently endogenous opioids and their receptor have been identified in a variety of reproductive and nonreproductive tissues outside the central nervous system. Their role within these tissues and organs, however, is only incompletely understood. In the central nervous system, endogenous opioids inhibit pulsatile GnRH release, in part mediating the stress response within the central nervous-pituitary gonadal axis, resulting in hypothalamic amenorrhea...
June 29, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28669364/expression-of-markers-for-germ-cells-and-oocytes-in-cow-dermal-fibroblast-treated-with-5-azacytidine-and-cultured-in-differentiation-medium-containing-bmp2-bmp4-or-follicular-fluid
#20
José Jackson do Nascimento Costa, Glaucinete Borges de Souza, Joyla Maria Pires Bernardo, Regislane Pinto Ribeiro, José Renato de Souza Passos, Francisco Taiã Gomes Bezerra, Márcia Viviane Alves Saraiva, José Roberto Viana Silva
This study aims to investigate the effect 5-azacytidine (5-Aza) during induction of pluripotency in bovine fibroblasts and to evaluate the effects of BMP2, BMP4 or follicular fluid in the differentiation of reprogrammed fibroblasts in primordial germ cells and oocytes. It also analysis the mRNA levels for OCT4, NANOG, REX, SOX2, VASA, DAZL, cKIT, SCP3, ZPA and GDF9 after culturing 5-Aza treated fibroblasts in the different tested medium. Dermal fibroblasts were cultured and exposed to 0.5, 1.0 or 2.0 μM of 5-Aza for 18 h, 36 h or 72 h...
June 2017: Zygote: the Biology of Gametes and Early Embryos
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