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https://www.readbyqxmd.com/read/29154179/the-effect-of-follicullar-fluid-pesticides-and-polychlorinated-biphenyls-concentrations-on-intracytoplasmic-sperm-injection-icsi-embryological-and-clinical-outcome
#1
Tarek K Al-Hussaini, Ahmed A Abdelaleem, Ihab Elnashar, Omar M Shabaan, Rashad Mostafa, Mona A H El-Baz, Sahar E M El-Deek, Tarek A Farghaly
OBJECTIVES: The present study evaluated the correlation between the concentrations of polychlorinated biphenyls (PCBs) and pesticides in the follicular fluid (FF) obtained during intracytoplasmic sperm injection (ICSI) with the ovarian response, endometrial thickness, and embryological and clinical outcomes. DESIGN: Cross-sectional observational study. MATERIALS AND METHODS: Women aged 20 to 38 years (300 infertile couples) presenting to a university-affiliated fertility center were approached to participate in the study...
November 15, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29150244/proteomic-analysis-of-follicular-fluid-from-tropically-adapted-goats
#2
Alexandre R Paula Junior, Mauricio F van Tilburg, Marina D P Lobo, Ana C O Monteiro-Moreira, Renato A Moreira, Carlos H S Melo, Joanna M G Souza-Fabjan, Airton A Araújo, Luciana M Melo, Dárcio I A Teixeira, Arlindo A Moura, Vicente J F Freitas
The present study was conducted to characterize the major proteome of ovarian follicular fluid from locally-adapted, "Canindé" goats in the northeast of Brazil. Eight estrous cycling goats received a hormonal treatment consisting of medroxyprogesterone acetate, D-cloprostenol and FSH. Fluid was collected by laparoscopy from small (<3mm), medium (3-4mm) and large (>4mm) follicles and then, proteins were analyzed by 2-D SDS-PAGE and tandem mass spectrometry. Thirty-six proteins were identified in the goat follicular fluid, including albumin, immunoglobulins, ceruloplasmin, complement factor B, alpha-1B-glycoprotein precursor, serotransferrin, complement C3 and serpins, among others...
November 6, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29149247/quantitative-proteomic-profiling-of-bovine-follicular-fluid-during-follicle-development
#3
Rodrigo de Andrade Ferrazza, Elizabeth Moreira Dos Santos Schmidt, Monika Mihm Carmichael, Fabiana Ferreira de Souza, Richard Burchmore, Roberto Sartori, Peter David Eckersall, João Carlos Pinheiro Ferreira
Bovine follicular fluid (FF) constitutes the microenvironment of follicles and includes various biologically active proteins. We performed a study involving 18 healthy non-lactating Holstein cows to determine the protein expression profile of FF at key-stages of follicular development. Follicles were individually aspirated in vivo at pre-deviation (F1∼7.0mm); deviation (F1∼8.5mm); post-deviation (F1∼12.0mm); and pre-ovulatory stages of follicle development, which were confirmed by measurement of follicular estradiol and progesterone concentrations...
November 15, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29134715/relationship-between-follicular-volume-and-oocyte-competence-blastocyst-development-and-live-birth-rate-optimal-follicle-size-for-oocyte-retrieval
#4
Barbara Wirleitner, Jasmin Okhowat, Lucie Vištejnová, Milena Králíčková, Marie Karlíková, Pierre Vanderzwalmen, Fabien Ectors, Libor Hradecký, Maximilian Schuff, Maximilian Murtinger
OBJECTIVES: To analyse the oocyte competence in GnRHa stimulation cycles with regard to maturity, fertilization and blastocyst rate and clinical outcome (pregnancy and live birth rate) in correlation to follicular volume measured by 3D sonography and follicular fluid composition. METHODS: This was a prospective single-centre study conducted in the period June 2012 - June 2014 including 118 ovum pick-ups (OPUs) with subsequent embryo transfers (ET). Out of 1,493 aspirated follicles we analysed 1,236 follicles and traced oocytes grouped according to follicular volume...
November 14, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29134695/acute-stimulation-of-a-smooth-muscle-constrictor-by-oestradiol-17%C3%AE-via-gper1-in-bovine-oviducts
#5
T Nishie, Y Kobayashi, K Kimura, K Okuda
Oviducts play roles in reproductive processes, including gametes transport, fertilization and early embryo development. Oviductal transport is controlled by various factors such as endothelins (EDNs) and nitric oxide (NO), smooth muscle contracting and relaxing factor, respectively. EDNs and NO production depend on an ovarian steroid hormone, oestradiol-17β (E2) and E2 quickly exerts their biological functions through G protein-coupled oestrogen receptor 1 (GPER1), which mediates rapid intracellular signalling...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29132734/role-of-vitamin-e-and-d3-supplementation-in-intra-cytoplasmic-sperm-injection-outcomes-of-women-with-polycystic-ovarian-syndrome-a-double-blinded-randomized-placebo-controlled-trial
#6
Farnaz Fatemi, Afsaneh Mohammadzadeh, Mohammad Reza Sadeghi, Mohammad Mehdi Akhondi, Shayan Mohammadmoradi, Koorosh Kamali, Nicknam Lackpour, Sheida Jouhari, Simin Zafadoust, Sara Mokhtar, Ladan Giahi
BACKGROUND & AIMS: Ovulation induction has been proven to impose oxidative stress during ICSI treatment cycle. Also, data indicates that PCOS women show higher oxidative markers. Available data are not convincing about which antioxidant supplements have the potential to overcome oxidative stress in PCOS subjects. The aim of this trial was to investigate the possible role of combined vitamin E and D supplementation in the ICSI outcomes (oocyte number and quality, embryo number and quality, pregnancy rate) of PCOS subjects...
April 2017: Clinical Nutrition ESPEN
https://www.readbyqxmd.com/read/29129796/comprehensive-profiling-of-prostaglandins-in-human-ovarian-follicular-fluid-using-mass-spectrometry
#7
Bruce Pier, Johnathan W Edmonds, Landon Wilson, Alireza Arabshahi, Ray Moore, G Wright Bates, Jeevan K Prasain, Michael A Miller
Prostaglandins are formed by enzymatic and non-enzymatic mechanisms. They have been detected in human ovarian follicular fluid (HFF), a medium rich in growth factors and nutrients important for oocyte growth and fertility. However, the comprehensive identification of HFF prostaglandins has not been addressed. Here we use hybrid triple quadrupole time-of-flight and triple quadrupole mass spectrometers to comprehensively analyze prostaglandins in HFF. We identified PGE1, PGE2, PGF2α, and other prostaglandins synthesized via prostaglandin-endoperoxide synthase (i...
November 9, 2017: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/29116087/differential-expression-of-mir-93-and-mir-21-in-granulosa-cells-and-follicular-fluid-of-polycystic-ovary-syndrome-associating-with-different-phenotypes
#8
Mohammad Naji, Ashraf Aleyasin, Saeid Nekoonam, Ehsan Arefian, Reza Mahdian, Fardin Amidi
The heterogeneous and multifactorial essence of polycystic ovary syndrome (PCOS) renders a remarkable significance to microRNAs (miRNAs). Normo-androgenic (NA) and hyperandrogenic (HA) PCOS patients were compared with matched healthy women. Expression of miRNAs and TGFβ signaling genes was studied by qRT-PCR and western blotting. Effect of androgen on expression of miR-93 and miR-21 and involvement of androgen receptor were appraised. In granulosa cells (GCs), miR-93 and miR-21 showed significantly increased levels in HA patients compared to NA patients...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29113318/follicular-metabolic-changes-and-effects-on-oocyte-quality-in-polycystic-ovary-syndrome-patients
#9
Yan Zhang, Lingyan Liu, Tai-Lang Yin, Jing Yang, Cheng-Liang Xiong
Polycystic ovary syndrome (PCOS) is a common complex and heterogeneous disorder, affecting up to 10% women at reproductive age. It causes three fourth of the ovulatory infertility and PCOS patients often give poor IVF quality. Although some metabolic profiles have been investigated in PCOS patient sera and urine, the follicular fluid, providing fruitful biochemical information about oocyte environment during development has been ignored. In this work, based on NMR metabolomics approach, metabolic profile of follicular fluid of PCOS patients has been explored and compared with healthy controls...
October 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/29095972/placental-growth-factor-is-required-for-ovulation-luteinization-and-angiogenesis-in-primate-ovulatory-follicles
#10
Hannah R Bender, Heidi A Trau, Diane M Duffy
Placental growth factor (PGF) is member of the vascular endothelial growth factor (VEGF) family of angiogenesis regulators. VEGFA is an established regulator of ovulation and formation of the corpus luteum. To determine if PGF also mediates aspects of ovulation and luteinization, macaques received gonadotropins to stimulate multiple follicular development. Ovarian biopsies and whole ovaries were collected before (0h) and up to 36h after hCG administration to span the ovulatory interval. PGF and VEGFA were expressed by both granulosa cells and theca cells...
October 30, 2017: Endocrinology
https://www.readbyqxmd.com/read/29080277/proteomic-profiling-of-follicle-fluids-after-superstimulation-in-one-month-old-lambs
#11
Y Wu, J Lin, B Han, L Wang, Y Chen, M Liu, J Huang
Follicular fluid (FF) accumulates in the antrum of the ovarian follicle. In addition, FF provides the microenvironment for oocyte development, oocyte maturation and competence, which are acquired during follicular development. Superstimulatory treatment of 1-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles are unable to completely mature and ovulate. Furthermore, the oocytes exhibit lower competence compared with those of ewes. In this study, we utilized an isobaric tag for relative and absolute quantification (iTRAQ)-based proteomics analysis and compare protein composition between pre-pubertal and adult superstimulated follicle FF in sheep...
October 28, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29080259/counting-ovarian-antral-follicles-by-ultrasound-a-practical-guide
#12
Marcela A Coelho Neto, Artur Ludwin, Antoni Borrell, Beryl Benacerraf, Didier Dewailly, Fabricio da Silva Costa, George Condous, Juan L Alcazar, Ligita Jokubkiene, Stefano Guerriero, Thierry Van den Bosch, Wellington P Martins
OBJECTIVE: To summarize the main aspects of several available techniques to perform ovarian antral follicle count (AFC), to propose a standardized report and to draw perspectives for future research on this subject. METHODS: This is a consensus paper from a group of clinicians and sonologists. The initial manuscript was written by three authors and then submitted to all the other authors asking for comments/corrections. After three versions incorporating suggestions, the fourth version of the manuscript was approved by all the authors...
October 28, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29071578/expression-of-mir-15a-mir-145-and-mir-182-in-granulosa-lutein-cells-follicular-fluid-and-serum-of-women-with-polycystic-ovary-syndrome-pcos
#13
Mohammad Naji, Saeid Nekoonam, Ashraf Aleyasin, Ehsan Arefian, Reza Mahdian, Elham Azizi, Maryam Shabani Nashtaei, Fardin Amidi
PURPOSE: Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies that affects women in reproductive age. MicroRNAs (miRNAs) play crucial roles in normal function of female reproductive system and folliculogenesis. Deregulated expression of miRNAs in PCOS condition may be significantly implicated in the pathogenesis of PCOS. We determined relative expression of miR-15a, miR-145, and miR-182 in granulosa-lutein cells (GLCs), follicular fluid (FF), and serum of PCOS patients...
October 25, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29069503/metabolomics-as-a-tool-to-identify-biomarkers-to-predict-and-improve-outcomes-in-reproductive-medicine-a-systematic-review
#14
Timothy Bracewell-Milnes, Srdjan Saso, Hossam Abdalla, Dimitrios Nikolau, Julian Norman-Taylor, Mark Johnson, Elaine Holmes, Meen-Yau Thum
BACKGROUND: Infertility is a complex disorder with significant medical, psychological and financial consequences for patients. With live-birth rates per cycle below 30% and a drive from the Human Fertilisation and Embryology Authority (HFEA) to encourage single embryo transfer, there is significant research in different areas aiming to improve success rates of fertility treatments. One such area is investigating the causes of infertility at a molecular level, and metabolomics techniques provide a platform for studying relevant biofluids in the reproductive tract...
November 1, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/29057034/oxidative-stress-and-endometriosis-a-systematic-review-of-the-literature
#15
REVIEW
Gennaro Scutiero, Piergiorgio Iannone, Giulia Bernardi, Gloria Bonaccorsi, Savino Spadaro, Carlo Alberto Volta, Pantaleo Greco, Luigi Nappi
Endometriosis is one of the most common gynaecologic diseases in women of reproductive age. It is characterized by the presence of endometrial tissue outside the uterine cavity. The women affected suffer from pelvic pain and infertility. The complex etiology is still unclear and it is based on three main theories: retrograde menstruation, coelomic metaplasia, and induction theory. Genetics and epigenetics also play a role in the development of endometriosis. Recent studies have put the attention on the role of oxidative stress, defined as an imbalance between reactive oxygen species (ROS) and antioxidants, which may be implicated in the pathophysiology of endometriosis causing a general inflammatory response in the peritoneal cavity...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29045296/taking-the-tube-from-normal-fallopian-tube-epithelium-to-ovarian-high-grade-serous-carcinoma
#16
Alicia A Tone
Detailed pathologic studies over the past decade suggest a distal fallopian tube origin for the majority of "ovarian" high-grade serous carcinomas (HGSC). This review will summarize molecular alterations observed in tubal precursors for HGSC, namely p53 signatures and serous tubal intraepithelial carcinomas, and in nonmalignant fallopian tube epithelial cells obtained from women at increased genetic risk for HGSC. Recent experiments investigating the impact of follicular fluid exposure and retrograde menstruation on tumor development in the fallopian tube will also be discussed...
December 2017: Clinical Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29043702/n-acetylcysteine-compared-to-metformin-improves-the-expression-profile-of-growth-differentiation-factor-9-and-receptor-tyrosine-kinase-c-kit-in-the-oocytes-of-patients-with-polycystic-ovarian-syndrome
#17
Ebrahim Cheraghi, Malek Soleimani Mehranjani, Seyed Mohammad Ali Shariatzadeh, Mohammad Hossein Nasr Esfahani, Behrang Alani
Background: Paracrine disruption of growth factors in women with polycystic ovarian syndrome (PCOS) results in production of low quality oocyte, especially following ovulation induction. The aim of this study was to investigate the effects of metformin (MET), N-acetylcysteine (NAC) and their combination on the hormonal levels and expression profile of GDF-9, BMP-15 and c-kit, as hallmarks of oocyte quality, in PCOS patients. MATERIALS AND METHODS: This prospective randomized, double-blind, placebo controlled trial aims to study the effects of MET, NAC and their combination (MET+NAC) on expression of GDF-9, BMP-15 and c-kit mRNA in oocytes [10 at the germinal vesicle (GV) stage, 10 at the MI stage, and 10 at the MII stage from per group] derived following ovulation induction in PCOS...
January 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29043003/tiaogeng-yijing-decoction-improves-the-pregnancy-outcomes-of-patients-with-poor-ovarian-response-undergoing-in-vitro-fertilization-embryo-transfer
#18
Hui Xue, Juan Dong, Huijun Xu, Xin Huang, Huangguan Dai, Cuifang Hao
The present study aimed to explore the therapeutic effects of the Tiaogeng Yijing decoction on patients with poor ovarian response (POR) undergoing in vitro fertilization-embryo transfer (IVF-ET), in addition to the underlying molecular mechanisms of these effects. A total of 40 patients were randomly and equally assigned to the treatment or control group. Patients in the treatment group received the Tiaogeng Yijing decoction continuously for three menstrual cycles in addition to microstimulation, while patients in the control group underwent microstimulation only...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29040513/targeted-metabolomics-reveals-reduced-levels-of-polyunsaturated-choline-plasmalogens-and-a-smaller-dimethylarginine-arginine-ratio-in-the-follicular-fluid-of-patients-with-a-diminished-ovarian-reserve
#19
J M Chao de la Barca, T Boueilh, G Simard, L Boucret, V Ferré-L'Hotellier, L Tessier, C Gadras, P E Bouet, P Descamps, V Procaccio, P Reynier, P May-Panloup
STUDY QUESTION: Does the metabolomic profile of the follicular fluid (FF) of patients with a diminished ovarian reserve (DOR) differ from that of patients with a normal ovarian reserve (NOR)? SUMMARY ANSWER: The metabolomic signature of the FF reveals a significant decrease in polyunsaturated choline plasmalogens and methyl arginine transferase activity in DOR patients compared to NOR patients. WHAT IS KNOWN ALREADY: The composition of the FF reflects the exchanges between the oocyte and its microenvironment during its acquisition of gametic competence...
November 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29039171/-correlation-of-fetuin-b-concentrations-in-serum-and-follicular-fluid-with-outcomes-of-in-vitro-fertilization
#20
Xiaorui Hu, Fengyi Mo, Qing Ma, Long Cui, Pingping Lyu, Yinghui Ye
OBJECTIVE: To investigate the relationship between serum/follicular fluid fetuin B levels and outcome of in vitro fertilization (IVF). METHODS: Infertility women (28 with low fertilization rates, 44 with normal fertilization rates) receiving IVF in Women's Hospital of Zhejiang University School of Medicine during June and December 2016 were enrolled in the study. Serum/follicular fluid fetuin B levels were measured with ELISA method. Correlations of serum and follicular fetuin B level with fertilization outcome of IVF were analyzed with Pearson correlation coefficient and receiver operating characteristic (ROC) curve...
May 25, 2017: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
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