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https://www.readbyqxmd.com/read/27900613/associations-between-follicular-fluid-high-density-lipoprotein-particle-components-and-embryo-quality-among-in-vitro-fertilization-patients
#1
K Kim, M S Bloom, R W Browne, E M Bell, R M Yucel, V Y Fujimoto
PURPOSE: Folliular redox balance is likely to be important for embryo quality during in vitro fertilization (IVF), and the anti-oxidative high desity lipoprotein (HDL) particle is the sole lipoprotein measured in follicular fluid (FF). Therefore, we investigated FF HDL particle components as predictors of embryo quality during IVF. METHODS: Two research follicles collected from each participant were individually tracked, and 103 women having at least one developed embryo were included in the analysis...
November 29, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27895531/follicular-fluid-biomarkers-for-human-in-vitro-fertilization-outcome-proof-of-principle
#2
Fang Chen, Carl Spiessens, Thomas D'Hooghe, Karen Peeraer, Sebastien Carpentier
BACKGROUND: Human follicular fluid (FF) is a unique biological fluid in which the oocyte develops in vivo, and presents an optimal source for non-invasive biochemical predictors. Oocyte quality directly influences the embryo development and hence, may be used as a predictor of embryo quality. Peptide profiling of FF and its potential use as a biomarker for oocyte quality has never been reported. METHODS: This study screened FF for peptide biomarkers that predict the outcome of in vitro fertilization (IVF)...
2016: Proteome Science
https://www.readbyqxmd.com/read/27889868/biobanking-of-different-body-fluids-within-the-frame-of-ivf-a-standard-operating-procedure-to-improve-reproductive-biology-research
#3
Michael Schenk, Berthold Huppertz, Barbara Obermayer-Pietsch, Darja Kastelic, Martina Hörmann-Kröpfl, Gregor Weiss
PURPOSE: The aim of the present study was to develop a standard operating procedure (SOP) for the collection, transport, and storage of human cumulus cells, follicular fluid, blood serum, seminal plasma, embryo culture supernatant, and embryo culture supernatant control obtained within the IVF process under approved protocols and written informed consent from participating patients. The SOP was developed at the Kinderwunsch Institut Schenk, Dobl, Austria, together with Biobank Graz of the Medical University of Graz, Austria...
November 26, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27884753/cell-free-dna-induced-apoptosis-of-granulosa-cells-by-oxidative-stress
#4
Yichun Guan, Wenjuan Zhang, Xingling Wang, Pengfei Cai, Qi Jia, Wenjie Zhao
BACKGROUND: Cell-free DNA is a DNA fragment that is produced by cell apoptosis which can affect the micro-environment of cell apoptosis. The levels of Cell-free DNA have been associated with successful rate of in vitro fertilization-embryo transfer (IVF-ET) and embryonic development. Our aim is to determine the relationship between cell-free DNA and embryo quality. The mechanisms of cell-free DNA in granulose and the apoptosis will be determined also. METHODS: The study enrolled patients who were undergone IVF for the first time and grouped the patients as pregnant (n=130) and non-pregnant (n=59)...
November 21, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27866059/glucocorticoid-metabolism-in-equine-follicles-and-oocytes
#5
D Scarlet, N Ille, R Ertl, B G Alves, G D A Gastal, S O Paiva, M O Gastal, E L Gastal, C Aurich
The objective of this study was to determine whether (1) systemic and intrafollicular cortisol concentrations in horses are directly related and (2) supraphysiological levels of glucocorticoids affect in vitro maturation (IVM) rates of oocytes. Specifically, we studied the (1) changes in the intrafollicular cortisol and progesterone in context with granulosa cell gene expression during maturation of equine follicles (from 5-9 mm, 10-14 mm, 15-19 mm, 20-24 mm, and ≥25 mm in diameter) and (2) effects of cortisol supplementation on IVM rates and gene expression of equine cumulus-oocyte complexes (COCs)...
October 29, 2016: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/27862406/expression-pattern-of-hif1alpha-and-vasohibins-during-follicle-maturation-and-corpus-luteum-function-in-the-bovine-ovary
#6
B Berisha, D Schams, D Rodler, F Sinowatz, M W Pfaffl
The aim of this study was to characterize expression patterns of hypoxia-inducible factor-1alpha (HIF1A) and vasohibin family members (VASH1 and VASH2) during different stages of ovarian function in cow. Experiment 1: Antral follicle classification occurred by follicle size and estradiol-17beta (E2) concentration in the follicular fluid into 5 groups (<0.5, 0.5-5, 5-40, 40-180 and >180 E2 ng/ml). Experiment 2: Corpora lutea (CL) were assigned to the following stages: days 1-2, 3-4, 5-7, 8-12, 13-16 and >18 (after regression) of oestrous cycle and of pregnancy (months 1-2, 3-4, 6-7, >8)...
November 11, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27854162/endometritis-increases-pro-inflammatory-cytokines-in-follicular-fluid-and-cervico-vaginal-mucus-in-the-buffalo-cow
#7
Jones Boby, Harendra Kumar, Harihar Prasad Gupta, Mustapha Hussain Jan, Sanjay Kumar Singh, Manas Kumar Patra, Sukdeb Nandi, Asha Abraham, Narayanan Krishnaswamy
Emerging evidence shows that some of the pro-inflammatory cytokines are elevated not only in the endometrium but also in the follicular fluid of cows with endometritis. Developing a cervico-vaginal mucus (CVM) based test has the potential for becoming a pen-side test because of the ease of sample collection. The present study describes the results of two different experiments. The first experiment was conducted to investigate the influence of endometritis on the proinflammatory cytokines of follicular fluid based on the reproductive tracts of buffalo collected at a slaughter house Buffalo genitalia were categorized into purulent endometritis (PE), cytological endometritis (CE), and non-endometritis (NE) based on the white-side test and endometrial cytology, respectively (n = 14/group)...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27843812/an-unusual-case-of-malignant-struma-ovarii-causing-thyrotoxicosis
#8
Elli Anagnostou, Antonios Polymeris, Georgios Morphopoulos, Alexios Travlos, Vassiliki Sarantopoulou, Irini Papaspyrou
BACKGROUND: Struma ovarii (SO) is a specialized monodermal teratoma predominantly composed of mature thyroid tissue, accounting for approximately 5% of all ovarian teratomas. Thyrotoxicosis is seen in about 8% of patients with SO. Most SO cases are benign with only 5-10% being malignant, and malignant SO causing thyrotoxicosis is very uncommon. CASE: A 64-year-old woman had been diagnosed with thyrotoxicosis 2 years previously. The thyroid gland was palpable with a micronodular texture, and the patient was euthyroid under carbimazole...
September 2016: European Thyroid Journal
https://www.readbyqxmd.com/read/27842549/acth-induced-stress-in-weaned-sows-impairs-lh-receptor-expression-and-steroidogenesis-capacity-in-the-ovary
#9
H S Zhu, Z Qian, H L Liu, E D Bao
BACKGROUND: Stress has been proved to impair the porcine reproduction soundly. Endocrine disruption, which is closely related to the persistent follicles, is possibly one of the results of stress, although the mechanism is unclear. Since the expression of luteinizing hormone receptor (LHR) in ovarian follicular wall and concentrations of steroid hormone in follicular fluid are related to the development of persistent follicles, this study is designed to evaluate the effect of administered adrenocorticotrophic hormone (ACTH) to weaned pigs on their ovarian steroidogenesis capacity and LHR expression...
November 14, 2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27817165/obesity-is-not-a-descriptive-factor-for-oxidative-stress-and-viscosity-in-follicular-fluid-of-in-vitro-fertilization-patients
#10
B Yuksel, S Kilic, N Yilmaz, T Goktas, U Keskin, A Seven, M Ulubay, S Batioglu
BACKGROUND: Obesity's impact on micro-environmental oxidative stress and follicular fluid (FF) viscosity and whether or not it has any effect on in vitro fertilization (IVF) success is a matter of debate. AIMS: In this study, our aim was to evaluate the levels of oxidative stress markers and the FF viscosity in obese and non-obese patients. METHODS: Eighty norm-responder patients undergoing IVF were prospectively grouped according to their body mass indexes (BMI)...
November 5, 2016: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/27802789/follicular-fluid-lipid-peroxidation-levels-in-women-with-endometriosis-during-controlled-ovarian-hyperstimulation
#11
Camila Bruna de Lima, Fernanda Bertuccez Cordeiro, Mariana Camargo, Daniel Suslik Zylbersztejn, Agnaldo Pereira Cedenho, Ricardo Pimenta Bertolla, Edson Guimarães Lo Turco
This observational study aimed to establishing a relationship between lipid peroxidation and endometriosis in women undergoing controlled ovarian hyperstimulation. A total of 79 women were divided into two groups: (i) controls (tubal or male factor); and (ii) endometriosis (stages III/IV). The endometriosis diagnosis was confirmed by videolaparoscopy and the controlled ovarian stimulation protocol was similar to all patients. Follicular fluid (FF) lipid peroxidation levels were determined through the quantification of malondialdehyde...
November 1, 2016: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/27799458/microrna-145-negatively-regulates-cell-proliferation-through-targeting-irs1-in-isolated-ovarian-granulosa-cells-from-patients-with-polycystic-ovary-syndrome
#12
Guoqing Cai, Xiangdong Ma, Biliang Chen, Yanhong Huang, Shujuan Liu, Hong Yang, Wei Zou
Polycystic ovary syndrome (PCOS) is a complex, heterogeneous endocrine and metabolic disorder affecting 5% to 10% of reproductive-age women. A high rate of granulosa cell (GC) proliferation contributes to the abnormal folliculogenesis in patients with PCOS. Evidence has proved that dysregulation of microRNAs is involved in the pathogenesis of PCOS. In this study, we investigated the effect of miR-145 on cell proliferation and the underlying mechanism of miR-145 in isolated human GCs from the aspirated follicular fluid in women with PCOS...
October 30, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27797914/oocyte-somatic-cell-interactions-in-the-human-ovary-novel-role-of-bone-morphogenetic-proteins-and-growth-differentiation-factors
#13
REVIEW
Hsun-Ming Chang, Jie Qiao, Peter C K Leung
BACKGROUND: Initially identified for their capability to induce heterotopic bone formation, bone morphogenetic proteins (BMPs) are multifunctional growth factors that belong to the transforming growth factor β superfamily. Using cellular and molecular genetic approaches, recent studies have implicated intra-ovarian BMPs as potent regulators of ovarian follicular function. The bi-directional communication of oocytes and the surrounding somatic cells is mandatory for normal follicle development and oocyte maturation...
October 26, 2016: Human Reproduction Update
https://www.readbyqxmd.com/read/27793387/effect-of-pregnancy-associated-plasma-protein-a-papp-a-single-nucleotide-polymorphisms-on-the-level-and-activity-of-papp-a-and-the-hormone-profile-in-fluid-from-normal-human-small-antral-follicles
#14
Jane Alrø Bøtkjær, Tanni Borgbo, Søren Kløverpris, Pernille Rimmer Noer, Claus Oxvig, Claus Yding Andersen
OBJECTIVE: To reveal a possible relationship between two single nucleotide polymorphisms (SNPs) in PAPP-A-1224 (rs7020782) and 327 (rs12375498)-and the level and activity of PAPP-A in follicular fluid (FF) of human small antral follicles, and to analyze the intrafollicular hormone levels. DESIGN: Laboratory investigation. SETTING: University hospital. PATIENT(S): Fifty volunteer women who contributed a total of 210 samples of FF from normal small antral follicles...
October 25, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27792259/in-situ-carcinoma-in-a-hybrid-cyst-a-case-report
#15
Kayhan Başak, Pınar Y Başak
Follicular hybrid cysts including two or more components of the epithelial skin adnexa are very rare. The epithelial lining of hybrid cysts varies, and either contains epidermis, and trichilemmal squamous epithelium, or other epithelia of the skin adnexa. Hybrid cysts may also be associated with neoplasia, such as in situ carcinoma, Bowen's disease and squamous cell carcinoma. A 37-year-old female was complaining of a cyst on her scalp. The unilocular cystic lesion was lined with markedly atypical squamous cells with trichilemmal differentiation...
October 28, 2016: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/27777587/myoinositol-improves-embryo-development-in-pcos-patients-undergoing-icsi
#16
Artur Wdowiak
The aim of this study was to investigate the activity of myoinositol, in a court of 217 PCOS women undergoing intracytoplasmic sperm injection (ICSI), on pregnancy rate, embryo development, estradiol, and progesterone concentration in blood serum, superoxide dismutase (SOD), and catalase (CAT) in follicular fluid. Concerning the court of patient, 112 (groups I and II) out of 217 were PCOS women, whereas group III consisted of healthy subjects (not PCOS). Group I patients were treated with 400 μg of folic acid per day for 3 months before ICSI, whereas group II patients received 4000 mg of myoinositol and 400 μg of folic acid per day for 3 months before ICSI...
2016: International Journal of Endocrinology
https://www.readbyqxmd.com/read/27773907/subacute-cerebellar-ataxia-with-amphiphysin-antibody-developing-in-a-patient-with-follicular-thyroid-adenoma-a-case-report
#17
Tomoki Hirunagi, Katsunori Sato, Masahiko Fujino, Keiko Tanaka, Yoji Goto, Kazuo Mano
The patient was a 61-year-old woman with thyroid enlargement since her 20s. She began to fall down repeatedly towards the end of June 2015. She was admitted to our hospital in the middle of August because of difficulty in walking. Upon admission, she presented with neck tremor and was unable to maintain a sitting position due to ataxia of the trunk and limbs. We studied serum anti-neuronal antibodies and obtained a positive result for anti-amphiphysin antibody (AMPH-Ab). Cerebrospinal fluid analysis revealed elevated protein levels and IgG index...
November 29, 2016: Rinshō Shinkeigaku, Clinical Neurology
https://www.readbyqxmd.com/read/27766972/insulin-concentrations-used-in-in-vitro-embryo-production-systems-a-pilot-study-on-insulin-stability-with-an-emphasis-on-concentrations-measured-in-vivo
#18
Denise Laskowski, Ylva Sjunnesson, Hans Gustafsson, Patrice Humblot, Göran Andersson, Renée Båge
BACKGROUND: Insulin has been used as a stimulatory factor for in vitro cell culture since many years. Even for routine in vitro embryo production (IVP), insulin is added to the media during different steps. There is a strong difference in concentrations used in vitro compared to what is measured in vivo in follicular fluid or serum. We performed a pilot study on insulin stability to explain possible reasons for that variation. RESULTS: We measured insulin concentrations before and after bovine oocyte maturation in an experiment by using a quantitative ELISA (Mercodia bovine insulin ELISA immunoassay) and found that concentrations were stable up to 22 h of incubation...
October 20, 2016: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/27761889/microrna-155-in-serum-derived-extracellular-vesicles-as-a-potential-biomarker-for-hematologic-malignancies-a-short-report
#19
Antonella Caivano, Francesco La Rocca, Vittorio Simeon, Marco Girasole, Simone Dinarelli, Ilaria Laurenzana, Angelo De Stradis, Luciana De Luca, Stefania Trino, Antonio Traficante, Giovanni D'Arena, Giovanna Mansueto, Oreste Villani, Giuseppe Pietrantuono, Luca Laurenti, Luigi Del Vecchio, Pellegrino Musto
PURPOSE: The use of extracellular vesicles (EVs) from body fluids as "liquid biopsies" is emerging as a promising approach for the diagnosis, prognosis and therapeutic monitoring of cancer patients. MicroRNA-155 (miR155), a non-coding transcript of the B-cell integration cluster (BIC) gene, has been reported to play a critical role in the pathogenesis of several types of hematologic malignancies (HMs) in which high miR155 levels have been found. At yet, however, the EV miR155 level and its putative clinical relevance in sera of HM patients have not been reported...
October 19, 2016: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/27756033/relationship-between-hair-and-salivary-cortisol-and-pregnancy-in-women-undergoing-ivf
#20
Adam J Massey, Bruce K Campbell, Nick Raine-Fenning, Catherine Pincott-Allen, Jane Perry, Kavita Vedhara
Evidence for an association between cortisol and clinical pregnancy in women undergoing In Vitro Fertilisation (IVF) is mixed with previous studies relying exclusively on short term measures of cortisol in blood, saliva, urine, and/or follicular fluid. Hair sampling allows analysis of systemic levels of cortisol over the preceding 3-6 months. The present study sought to explore the relationship between cortisol and clinical pregnancy outcome in women undergoing IVF utilising multiple indices of cortisol derived from both saliva and hair measured prior to commencing gonadotrophin treatment...
August 31, 2016: Psychoneuroendocrinology
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