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https://www.readbyqxmd.com/read/28634068/investigation-of-changes-in-endocannabinoids-and-n-acylethanolamides-in-biofluids-and-their-correlations-with-female-infertility
#1
Jun Ding, Xiao-Tong Luo, Yan-Ru Yao, Hua-Ming Xiao, Ming-Quan Guo
Female infertility is a worldwide medical problem, and the scarcity of infertility biomarkers has hindered the ability to launch preventive and therapeutic measures in a timely manner. Intriguingly, alterations in endocannabinoids (eCBs) and N-acylethanolamides (NAEs) have been observed in the biofluids of infertile females. Therefore, a hypothesis of using eCB and NAEs in biofluids as infertility biomarkers was proposed by several researchers; however, little evidence exists to verify the hypothesis. To investigate their correlations with female infertility, we developed a magnetic liquid microextraction-chemical derivatization (MLME-CD) method coupled with liquid chromatography-tandem mass spectrometry for the quantification of eCBs and NAEs in biofluids...
June 12, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28625274/beneficial-effects-of-melatonin-on-in-vitro-embryo-production-from-juvenile-goat-oocytes
#2
Sandra Soto-Heras, Montserrat Roura, Maria G Catalá, Irene Menéndez-Blanco, Dolors Izquierdo, Ali A Fouladi-Nashta, Maria T Paramio
Melatonin is a universal antioxidant that improves in vitro embryo production in several species. The aims of this study were to determine the melatonin concentration in the ovarian follicular fluid (FF) of juvenile goats and the effect of melatonin during in vitro maturation (IVM) on embryo development. The FF melatonin concentration was 0.57--1.07×10-9 M, increasing with follicular diameter. Oocytes were matured, fertilised and cultured under conventional conditions. Blastocyst development, embryo quality and levels of reactive oxygen species (ROS) and reduced glutathione were assessed...
June 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28621496/adrenomedullin-regulates-the-speed-of-oviductal-fluid-flow-in-cattle
#3
Yuka Yoshimoto, Takumi Nishie, Sayaka Ito, Yoshihiko Kobayashi, Yuki Yamamoto, Kiyoshi Okuda, Koji Kimura
Unidirectional flow of oviductal fluid from the ovarian to uterine side of the ampulla plays a significant role in successful pregnancy, and is produced by ciliary beating. Various systems regulate ciliary beating, such as paracrine, autocrine, and endocrine. We hypothesized that Adrenomedullin (ADM) - a peptide hormone that acts via its receptors, which are complexes of Calcitonin receptor-like receptor (CRLR) and Receptor activity-modifying protein (RAMP) 2 or 3-promotes oviductal fluid flow in the ampulla of bovine oviducts...
June 16, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28617821/supplementation-with-small-extracellular-vesicles-from-ovarian-follicular-fluid-during-in-vitro-production-modulates-bovine-embryo-development
#4
Juliano C da Silveira, Gabriella M Andrade, Maite Del Collado, Rafael V Sampaio, Juliano R Sangalli, Luciano A Silva, Fábio V L Pinaffi, Izabelle B Jardim, Marcelo C Cesar, Marcelo F G Nogueira, Aline S M Cesar, Luiz L Coutinho, Rinaldo W Pereira, Felipe Perecin, Flávio V Meirelles
Pregnancy success results from the interaction of multiple factors, among them are folliculogenesis and early embryonic development. Failure during these different processes can lead to difficulties in conception. Alternatives to overcome these problems are based on assisted reproductive techniques. Extracellular vesicles are cell-secreted vesicles present in different body fluids and contain bioactive materials, such as messenger RNA, microRNAs (miRNAs), and proteins. Thus, our hypothesis is that extracellular vesicles from follicular fluid from 3-6 mm ovarian follicles can modulate bovine embryo development in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28607476/zinc-finger-gene-217-znf217-promoted-ovarian-hyperstimulation-syndrome-ohss-through-regulating-e2-synthesis-and-inhibiting-thrombospondin-1-tsp-1
#5
Junyu Zhai, Jiansheng Liu, Xiaoyue Cheng, Shang Li, Yan Hong, Kang Sun, Zi-Jiang Chen, Yanzhi Du, Weiping Li
Zinc finger gene 217 (ZNF217) is a candidate gene of polycystic ovary syndrome (PCOS) which is vulnerable to ovarian hyperstimulation syndrome (OHSS). However, the relationship between ZNF217 and OHSS is largely unknown. Our study demonstrated that ZNF217 was mainly distributed in the granulosa cells of rat ovary. Significantly higher expression of ovarian ZNF217 was detected in OHSS rats, being consistent with serum 17β-estradiol concentration and ovarian aromatase. Moreover, OHSS rats also showed decreased ovarian TSP-1 mRNA, an acknowledged VEGF signaling suppressor...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28595033/temperature-gradients-in-vivo-influence-maturing-male-and-female-gametes-in-mammals-evidence-from-the-cow
#6
R H F Hunter, F López-Gatius, O López-Albors
Since 1980 several reports have indicated that temperatures vary between preovulatory follicles and other ovarian tissues in rabbit, cow, pig and human. However, these observations did not achieve prominence; they were regarded as artefacts due to the use of anaesthetics and open surgery (laparotomy). Recently, without resorting to anaesthesia or surgery, direct measurements of temperature in preovulatory follicles have been performed in the cow by means of a thermistor probe introduced into the antrum under ultrasonic guidance...
June 9, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28585716/detection-of-dendritic-cells-and-related-cytokines-in-follicular-fluid-of-patients-with-polycystic-ovary-syndrome
#7
Tao Zhang, Fuying Tian, Ran Huo, Aifa Tang, Yong Zeng, Yong-Gang Duan
PROBLEM: The presence of dendritic cells (DCs) and associated cytokines in follicular fluid (FF) from patients with polycystic ovary syndrome (PCOS) remains unknown. METHODS OF STUDY: FF was collected from PCOS patients and patients with severe male factor infertility (control) at the day of transvaginal oocyte retrieval. Phenotypes of DC were detected by flow cytometry, and TNF-α, IL-6, IL-10, and IL-23 were assessed by ELISA. RESULTS: A significant decrease in the percentage of DC was found in patients with PCOS (16...
June 6, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28583593/altered-expression-of-cytokines-il-1%C3%AE-il-6-il-8-and-tnf-%C3%AE-in-bovine-follicular-persistence
#8
A F Stassi, M E Baravalle, E M Belotti, F Rey, N C Gareis, P U Díaz, F M Rodríguez, C J Leiva, H H Ortega, N R Salvetti
In dairy cattle, cystic ovarian disease (COD) is an important cause of subfertility, and two of the main signs are ovulation failure and follicular persistence. The aim of this study was to examine the expression of the cytokines IL-1α, IL-6, IL-8 and TNF-α in ovarian follicular structures at different times of persistence in a model of follicular persistence induced by prolonged treatment with progesterone in dairy cows. Protein expression of IL-1α, IL-6, IL-8 and TNF-α was evaluated by immunohistochemistry...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28561185/high-leptin-level-attenuates-embryo-development-in-overweight-obese-infertile-women-by-inhibiting-proliferation-and-promotes-apoptosis-in-granule-cell
#9
Xian-Hua Lin, Hui Wang, Dan-Dan Wu, Kamran Ullah, Tian-Tian Yu, Tanzil Ur Rahman, He-Feng Huang
Obesity appears to be associated with female reproductive dysfunction and infertility. Women with obesity undergoing in vitro fertilization (IVF) had poor oocyte quality, decreased embryo development, and poor pregnancy outcome. However, the mechanism linking obesity to poor reproductive outcomes is still unclear. Obesity is frequently accompanied with elevated leptin levels. Here we aimed to evaluate the effect of high leptin level in follicular fluid (FF) on the proliferation and apoptosis in granule cells and correlate these findings with poor reproductive outcomes in infertile women with overweight or obesity who underwent IVF treatment...
May 30, 2017: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/28558842/natriuretic-peptides-improve-the-developmental-competence-of-in-vitro-cultured-porcine-oocytes
#10
Yanhao Zhang, Huarong Wang, Wei Liu, Ye Yang, Xiao Wang, Zhiyuan Zhang, Qirui Guo, Chao Wang, Guoliang Xia
BACKGROUND: Natriuretic peptides (NPs), brain and C type NPs (BNP and CNP), were involved in the maintenance of porcine oocyte meiotic arrest. The present study investigated the effects of NPs on developmental competence of immature porcine oocytes with follicles of different sizes. METHODS: Follicular fluid NP levels were examined by radioimmunoassay. The developmental competence of porcine oocytes was evaluated by cleavage and blastocyst developmental rates after in vitro fertilization (IVF) or parthenogenetic activation (PA) of cumulus oocyte complexes (COCs), which were recovered from follicle with different sizes...
May 30, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28553318/non-coding-rnas-in-the-ovarian-follicle
#11
Rosalia Battaglia, Maria E Vento, Placido Borzì, Marco Ragusa, Davide Barbagallo, Desirée Arena, Michele Purrello, Cinzia Di Pietro
The mammalian ovarian follicle is the complex reproductive unit comprising germ cell, somatic cells (Cumulus and Granulosa cells), and follicular fluid (FF): paracrine communication among the different cell types through FF ensures the development of a mature oocyte ready for fertilization. This paper is focused on non-coding RNAs in ovarian follicles and their predicted role in the pathways involved in oocyte growth and maturation. We determined the expression profiles of microRNAs in human oocytes and FF by high-throughput analysis and identified 267 microRNAs in FF and 176 in oocytes...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28542500/uterine-and-systemic-inflammation-influences-ovarian-follicular-function-in-postpartum-dairy-cows
#12
Soon Hon Cheong, Ocilon G Sá Filho, Victor A Absalon-Medina, Augusto Schneider, W R Butler, Robert O Gilbert
The objective of this study was to determine the effects of uterine and systemic inflammatory responses to uterine bacterial contamination at calving in dairy cows on the growth and ovulatory outcomes of the first dominant follicle postpartum. Ovulatory capability of the first dominant follicle postpartum was predicted in 53 multiparous cows by using a combination of follicle growth characteristics and circulating estradiol concentrations. Endotoxin levels were assayed in follicular fluid samples that were aspirated the day after ovulatory outcome prediction...
2017: PloS One
https://www.readbyqxmd.com/read/28535578/coasting-withholding-gonadotrophins-for-preventing-ovarian-hyperstimulation-syndrome
#13
REVIEW
Arianna D'Angelo, Nazar N Amso, Rudaina Hassan
BACKGROUND: Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic and potentially life threatening condition resulting from excessive ovarian stimulation. Reported incidence of moderate to severe OHSS ranges from 0.6% to 5% of in vitro fertilization (IVF) cycles. The factors contributing to OHSS have not been completely explained. The release of vasoactive substances secreted by the ovaries under human chorionic gonadotrophin (hCG) stimulation may play a key role in triggering this syndrome...
May 23, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28532828/expression-of-apelin-and-apelin-receptor-apj-in-porcine-ovarian-follicles-and-in%C3%A2-vitro-effect-of-apelin-on-steroidogenesis-and-proliferation-through-apj-activation-and-different-signaling-pathways
#14
A Rak, E Drwal, Ch Rame, K Knapczyk-Stwora, M Słomczyńska, J Dupont, E L Gregoraszczuk
Apelin was thought to be an adipocyte-specific hormone, but recent studies indicate a link between apelin and female reproductive function. Using real-time PCR, immunoblotting, immunohistochemistry and ELISA, we demonstrated expression of apelin and its receptor (APJ) in ovarian follicles of different sizes from mature pigs. Apelin concentration in the follicular fluid, and expression of both apelin and APJ, increased with follicular growth; greatest values were found in large follicles. Immunohistochemistry revealed the positive staining for apelin and APJ in membranes of granulosa, than theca cells...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28532823/effects-of-rumen-protected-methionine-and-choline-supplementation-on-steroidogenic-potential-of-the-first-postpartum-dominant-follicle-and-expression-of-immune-mediators-in-holstein-cows
#15
D A V Acosta, M I Rivelli, C Skenandore, Z Zhou, D H Keisler, D Luchini, M N Corrêa, F C Cardoso
Multiparous Holstein cows were assigned in a randomized complete block design into four treatments from 21 d before calving to 30 d in milk (DIM). Treatments were: MET [n = 19, fed the basal diet + rumen-protected methionine at a rate of 0.08% (w/w) of the dry matter, Smartamine(®) M], CHO (n = 17, fed the basal diet + choline 60 g/d, Reashure(®)), MIX (n = 21, fed the basal diet + Smartamine(®) M at a rate of 0.08% (w/w) of the dry matter and 60 g/d Reashure(®)), and CON (n = 20, no supplementation, fed the close-up and fresh cow diets)...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28524204/correction-metabolomic-profiling-and-biochemical-evaluation-of-the-follicular-fluid-of-endometriosis-patients
#16
Marianna Santonastaso, Alessia Pucciarelli, Susan Costantini, Francesca Caprio, Angela Sorice, Francesca Capone, Antonella Natella, Patrizia Iardino, Nicola Colacurci, Emilio Chiosi
Correction for 'Metabolomic profiling and biochemical evaluation of the follicular fluid of endometriosis patients' by Marianna Santonastaso et al., Mol. BioSyst., 2017, DOI: 10.1039/c7mb00181a.
May 19, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28520723/follicular-size-predicts-success-in-artificial-insemination-with-frozen-thawed-sperm-in-donkeys
#17
Joseph Saragusty, Alemayehu Lemma, Thomas Bernd Hildebrandt, Frank Göritz
In asses, semen collection, cryopreservation, and artificial insemination (AI) with frozen-thawed semen have been scarcely described and success rate, particularly following AI, is reportedly low. In the absence of reliable protocols, assisted reproductive technologies cannot support the conservation efforts aimed at endangered wild ass species and domestic donkey breeds. Two experiments were conducted in this study. In experiment 1 we evaluated freezing Abyssinian donkey (N = 5, 4 ejaculates each) spermatozoa using three freezing extenders (Berliner Cryomedium + glycerol, BC+G; BotuCrio, BOTU; INRAFreeze, INRA) and two cryopreservation techniques (liquid nitrogen vapour, LNV; directional freezing, DF)...
2017: PloS One
https://www.readbyqxmd.com/read/28512792/expression-of-aquaporin-4-in-the-chicken-ovary-in-relation-to-follicle-development
#18
M Nowak, M Grzesiak, N Saito, M Kwaśniewska, A Sechman, A Hrabia
In the mammalian ovary, aquaporins (AQPs) are thought to be involved in the regulation of fluid transport within the follicular wall and antrum formation. Data concerning the AQPs in the avian ovary is very limited. Therefore, the present study was designed to examine whether the AQP4 is present in the chicken ovary, and if so, what is its distribution in the ovarian compartment of the laying hen. Localization of AQP4 in the ovarian follicles at different stage of development was also investigated. After decapitation of hens the stroma with primordial follicles and white (1-4 mm), yellowish (4-8 mm), small yellow and the three largest yellow pre-ovulatory follicles F3-F1 (F3 < F2 < F1; 20-36 mm) were isolated from the ovary...
May 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28499814/selected-cholesterol-biosynthesis-inhibitors-produce-accumulation-of-the-intermediate-ff-mas-that-targets-nucleus-and-activates-lxr%C3%AE-in-hepg2-cells
#19
Leonardo Gatticchi, Bruno Cerra, Paolo Scarpelli, Lara Macchioni, Bartolomeo Sebastiani, Antimo Gioiello, Rita Roberti
Sterol intermediates of the cholesterol biosynthetic pathway have drawn attention for novel biological activities. Follicular fluid meiosis activating sterol (FF-MAS) is a LXRα ligand and a potential modulator of physiologic processes regulated by nuclear receptors, such as lipid homeostasis and cell proliferation. In this work, we established a model to selectively accumulate FF-MAS in HepG2 cells, by using a combination of the inhibitors AY9944 and 17-hydroxyprogesterone to block C14-sterol reductases and the downstream C4-demethylase complex...
May 9, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28496019/age-associated-changes-in-granulosa-cells-and-follicular-fluid-in-cows
#20
Hisataka Iwata
Age-associated decline in oocyte quality is common in mammals. Oocytes take a long time to reach their full-grown size in large animals, and maternal physical conditions profoundly affect follicle development. Aging affects the oocyte itself as well as the surrounding environment, such as granulosa cells and follicular fluid. This review discusses age-associated changes that occur in granulosa cells and follicular fluid in cows and suggests that age-associated decline in granulosa cells and follicular fluid hampers proper oocyte development...
May 12, 2017: Journal of Reproduction and Development
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