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https://www.readbyqxmd.com/read/28732041/understanding-russell-s-viper-venom-factor-v-activator-s-substrate-specificity-by-surface-plasmon-resonance-and-in-silico-studies
#1
Pradeep K Yadav, Christian B Antonyraj, Syed Ibrahim Basheer Ahamed, Sistla Srinivas
Blood coagulation factor V (FV) is activated either by Factor X or thrombin, cleaving at three different sites viz., Site I (Arg709-Ser710), site II (Arg1018-Thr1019), and site III (Arg1545-Ser1546). Russell's viper venom factor V activator (RVV-V) is a thrombin-like serine proteinase that activates FV with selective, single cleavage at site III. A long lasting effort is being pending in understanding the 'selective' binding specificity of the RVV-V towards site III. Here, we present the binding kinetic study of RVV-V with two designed peptides corresponding to the regions from site I (Gln699-Asn713) and site II (1008Lys-Pro1022), respectively, that include 15 amino acids...
2017: PloS One
https://www.readbyqxmd.com/read/28731772/publisher-s-note-infrared-topological-plasmons-in-graphene-phys-rev-lett-118-245301-2017
#2
Dafei Jin, Thomas Christensen, Marin Soljac Ić, Nicholas X Fang, Ling Lu, Xiang Zhang
This corrects the article DOI: 10.1103/PhysRevLett.118.245301.
July 7, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28731759/single-molecule-investigation-of-energy-dynamics-in-a-coupled-plasmon-exciton-system
#3
Hiroshi Imada, Kuniyuki Miwa, Miyabi Imai-Imada, Shota Kawahara, Kensuke Kimura, Yousoo Kim
We investigate the near-field interaction between an isolated free-base phthalocyanine molecule and a plasmon localized in the gap between an NaCl-covered Ag(111) surface and the tip apex of a scanning tunneling microscope. When the tip is located in the close proximity of the molecule, asymmetric dips emerge in the broad luminescence spectrum of the plasmon generated by the tunneling current. The origin of the dips is explained by energy transfer between the plasmon and molecular excitons and a quantum mechanical interference effect, where molecular vibrations provide additional degrees of freedom in the dynamic process...
July 7, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28731574/rapid-buffer-and-ligand-screening-for-affinity-chromatography-by-multiplexed-surface-plasmon-resonance-imaging
#4
Karin P M Geuijen, Daniëlle E J W van Wijk-Basten, David F Egging, Richard B M Schasfoort, Michel H Eppink
Protein purifications are often based on the principle of affinity chromatography, where the protein of interest selectively binds to an immobilized ligand. The development of affinity purification requires selecting proper wash and elution conditions. In recent years, miniaturization of the purification process is applied to speed up the development (e.g. microtiterplates, robocolumns). We have studied the application of surface plasmon resonance imaging (SPRi) as a tool to simultaneously screen many buffer conditions for wash and elution steps in an affinity-based purification process...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731438/evidence-that-the-human-innate-immune-peptide-ll-37-may-be-a-binding-partner-of%C3%A2-amyloid-%C3%AE-and-inhibitor-of%C3%A2-fibril-assembly
#5
Ersilia De Lorenzi, Marcella Chiari, Raffaella Colombo, Marina Cretich, Laura Sola, Renzo Vanna, Paola Gagni, Federica Bisceglia, Carlo Morasso, Jennifer S Lin, Moonhee Lee, Patrick L McGeer, Annelise E Barron
BACKGROUND: Identifying physiologically relevant binding partners of amyloid-β (Aβ) that modulate in vivo fibril formation may yield new insights into Alzheimer's disease (AD) etiology. Human cathelicidin peptide, LL-37, is an innate immune effector and modulator, ubiquitous in human tissues and expressed in myriad cell types. OBJECTIVE: We present in vitro experimental evidence and discuss findings supporting a novel hypothesis that LL-37 binds to Aβ42 and can modulate Aβ fibril formation...
July 18, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28731110/a-redox-controlled-electrolyte-for-plasmonic-enhanced-dye-sensitized-solar-cells
#6
Yuqiao Fu, Siu-Pang Ng, Guangyu Qiu, Tak-Fu Hung, Chi-Man Lawrence Wu, Chun-Sing Lee
Plasmonic enhanced dye-sensitized solar cells (DSSCs) with metallic nanostructures suffer from corrosion problems, especially with the presence of the iodine/triiodide redox couple in the electrolyte. Herein, we introduce an alternative approach by compensating the corrosion with a modified liquid electrolyte. In contrast to the existing method of surface preservation for plasmonic nanostructures, the redox-controlled electrolyte (RCE) contains iodoaurate intermediates, i.e. gold(i) diiodide (AuI2(-)) and gold(iii) tetraiodide (AuI4(-)) with optimal concentrations, such that these intermediates are readily reduced to gold nanoparticles during the operation of DSSCs...
July 21, 2017: Nanoscale
https://www.readbyqxmd.com/read/28730997/improving-the-electrical-contact-at-a-pt-tio2-nanowire-interface-by-selective-application-of-focused-femtosecond-laser-irradiation
#7
Songling Xing, Luchan Lin, Guisheng Zou, Lei Liu, Peng Peng, Aiping Wu, Walter W Duley, Norman Zhou
In this paper, we show that tightly focused femtosecond laser irradiation is effective in improving nanojoining of an oxide nanowire (TiO2) to a metal electrode (Pt), and how this process can be used to modify contact states. Enhanced chemical bondings are created due to localized plasmonically enhanced optical absorption at the Pt/TiO2 interface as confirmed by finite element simulations of the localized field distribution during irradiation. Nano Auger electron spectroscopy shows that the resulting heterojunction is depleted in oxygen, suggesting that a TiO2-x layer is formed between the Pt electrode and the TiO2 nanowire...
July 21, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28730812/potential-scanning-localized-plasmon-sensing-with-single-and-coupled-gold-nanorods
#8
Tokuhisa Kawawaki, Heyou Zhang, Hiroyasu Nishi, Paul Mulvaney, Tetsu Tatsuma
Single plasmonic nanoparticles can potentially serve as optical sensors for detecting local refractive index changes. However, simultaneous monitoring of the scattering spectra from multiple nanoparticles is not practical. Here we perform potential-scanning localized surface plasmon resonance (LSPR) sensing. Gold nanorods are immobilized on an ITO electrode. Instead of collecting the full spectrum, as is done in conventional LSPR sensing, the electrode potential is scanned while the rod spectra are monitored at a single wavelength...
July 21, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28730725/targeting-nano-drug-delivery-to-cancer-cells-using-tunable-multi-layer-silver-decorated-gold-nanorods
#9
Zeid A Nima, Ahmed M Alwbari, Vijayalakshmi Dantuluri, Rabab N Hamzah, Natasha Sra, Pooja Motwani, Konstantinos Arnaoutakis, Rebecca A Levy, Amani F Bohliqa, Dmitry Nedosekin, Vladimir P Zharov, Issam Makhoul, Alexandru S Biris
Multifunctional nanoparticles have high potential as targeting delivery vehicles for cancer chemotherapy. In this study, silver-decorated gold nanorods (AuNR\Ag) have been successfully used to deliver specific, targeted chemotherapy against breast cancer (MCF7) and prostate carcinoma (PC3) cell lines. Doxorubicin, a commonly used chemotherapy, and anti-Epithelial cell adhesion molecule (anti-EpCAM) antibodies were covalently bonded to thiolated polyethylene glycol-coated AuNR\Ag, and the resultant system was used to deliver the drugs to cancer cells in vitro...
July 21, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28730357/biosynthesis-of-silver-nanoparticles-from-spirulina-microalgae-and-its-antibacterial-activity
#10
Govarthanan Muthusamy, Selvankumar Thangasamy, Mythili Raja, Sudhakar Chinnappan, Selvam Kandasamy
The present work focuses on a low-cost, simple, and green synthesis of silver nanoparticles (AgNPs) by mixing AgNO3 solution with the extract of Spirulina platensis (SP) without any chemical reducing and/or capping agents. The green synthesis of AgNPs was confirmed by the color change from colorless to yellowish brown. The biosynthesis of AgNPs was further confirmed by UV-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), biological transmission electron microscopy (Bio-TEM), and energy dispersive X-ray analysis (EDX)...
July 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28729648/a-haplotype-in-cfh-family-genes-confers-high-risk-of-rare-glomerular-nephropathies
#11
Yin Ding, Weiwei Zhao, Tao Zhang, Hao Qiang, Jianping Lu, Xin Su, Shuzhen Wen, Feng Xu, Mingchao Zhang, Haitao Zhang, Caihong Zeng, Zhihong Liu, Huimei Chen
Despite distinct renal lesions, a series of rare glomerular nephropathies are reportedly mediated by complement overactivation. Genetic variations in complement genes contribute to disease risk, but the relationship of genotype to phenotype has not been straightforward. Here, we screened 11 complement genes from 91 patients with atypical hemolytic uremic syndrome (aHUS), C3 glomerulopathy (C3G) and membranoproliferative glomerulonephritis type I (MPGN I), and identified the concomitant presence of three missense variations located within the human complement Factor H (CFH) gene cluster...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729645/nano-antenna-enhanced-two-focus-fluorescence-correlation-spectroscopy
#12
Lutz Langguth, Agata Szuba, Sander A Mann, Erik C Garnett, Gijsje H Koenderink, A Femius Koenderink
We propose two-focus fluorescence correlation spectroscopy (2fFCS) on basis of plasmonic nanoantennas that provide distinct hot spots that are individually addressable through polarization, yet lie within a single diffraction limited microscope focus. The importance of two-focus FCS is that a calibrated distance between foci provides an intrinsic calibration to derive diffusion constants from measured correlation times. Through electromagnetic modelling we analyze a geometry of perpendicular nanorods, and their inverse, i...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729629/assembly-of-3d-plasmonic-clusters-by-2d-afm-nanomanipulation-of-highly-uniform-and-smooth-gold-nanospheres
#13
Kyung Jin Park, Ji-Hyeok Huh, Dae-Woong Jung, Jin-Sung Park, Gwan H Choi, Gaehang Lee, Pil J Yoo, Hong-Gyu Park, Gi-Ra Yi, Seungwoo Lee
Atomic force microscopy (AFM) nanomanipulation has been viewed as a deterministic method for the assembly of plasmonic metamolecules because it enables unprecedented engineering of clusters with exquisite control over particle number and geometry. Nevertheless, the dimensionality of plasmonic metamolecules via AFM nanomanipulation is limited to 2D, so as to restrict the design space of available artificial electromagnetisms. Here, we show that "2D" nanomanipulation of the AFM tip can be used to assemble "3D" plasmonic metamolecules in a versatile and deterministic way by dribbling highly spherical and smooth gold nanospheres (NSs) on a nanohole template rather than on a flat surface...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28727918/rapid-sensitive-and-quantitative-detection-of-anti-peg-antibodies-by-mpeg-coated-spr-sensors
#14
Peng Zhang, Fang Sun, Hsiang-Chieh Hung, Priyesh Jain, Kasey Joanne Leger, Shaoyi Jiang
Pre-existing and induced anti-poly(ethylene glycol) (PEG) antibodies (abs) have been shown to be related with limitation of therapeutic efficacy and reduction in tolerance of several therapeutic agents. However, the current methods to detect anti-PEG abs are tedious and usually lack of quantification. A facile, rapid, sensitive and reliable technique to detect anti-PEG abs is highly desired in both research and clinic settings. In this work, we have presented a surface plasmon resonance (SPR) biosensor technique for the detection of anti-PEG abs and compared three PEG surface chemistries...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727443/plasmonic-thermal-decomposition-digestion-of-proteins-a-rapid-on-surface-protein-digestion-technique-for-mass-spectrometry-imaging
#15
Rong Zhou, Franco Basile
A method based on plasmon surface resonance absorption and heating was developed to perform a rapid on-surface protein thermal decomposition and digestion suitable for imaging MS and/or profiling. This photo-thermal process or plasmonic thermal decomposition/digestion (plasmonic-TDD) method incorporates a continuous wave (CW) laser excitation and gold nanoparticles (Au-NPs) to induce known thermal decomposition reactions that cleave peptides and proteins specifically at the C-terminus of aspartic acid and at the N-terminus of cysteine...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727434/ellipsometry-of-colloidal-solutions-new-experimental-setup-and-application-to-metallic-colloids
#16
Yann Battie, Michel Stchakovsky, Aotmane En Naciri, Suzanna Akil, Nouari Chaoui, Laurent Broch
An ellipsometric cell is developed to simultaneously determine the shape distribution, the volume fraction, and the complex refractive index of gold and silver colloids. Simulation reveals that this cell drastically improves the detection limit of ellipsometry. Indeed, Ag and Au nanoparticles (NPs) are detected at the ppmv level. We demonstrate that the NPs shape distribution can be estimated from ellipsometric measurements by analyzing them with a shape distributed effective medium theory (SDEMT). The obtained distributions from ellipsometry are in agreement with those deduced from transmission electron microcopy (TEM)...
July 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28727409/plasmonic-nano-oven-by-concatenation-of-multishell-photo-thermal-enhancement
#17
Lijun Meng, Renwen Yu, Min Qiu, F Javier Garcia de Abajo
Metallodielectric multishell nanoparticles are capable of hosting collective plasmon oscillations distributed among different metallic layers, which result in large near-field enhancement at specific regions of the structure, where light absorption is maximized. We exploit this capability of multishell nanoparticles, combined with thermal boundary resistances and spatial tailoring of the optical near fields, to design plasmonic nano-ovens capable of achieving high temperatures at the core region using moderate illumination intensities...
July 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/28727320/plasmonic-gold-nanostars-incorporated-into-high-efficiency-perovskite-solar-cells
#18
Munkhbayar Batmunkh, Thomas Macdonald, William Peveler, Abdulaziz Bati, Claire J Carmalt Claire J Carmalt, Ivan Parkin, Joseph George Shapter
Incorporating appropriate plasmonic nanostructures into photovoltaic (PV) systems is of great utility for enhancing photon absorption and thus improving device performance. Herein, the successful integration of plasmonic gold nanostars (AuNSs) into mesoporous TiO2 photoelectrodes for perovskite solar cells (PSCs) is reported. The PSCs fabricated with TiO2-AuNSs photoelectrodes exhibited a device efficiency of up to 17.72%, while the control cells without AuNSs showed a maximum efficiency of 15.19%. We attribute the origin of increased device performance to enhanced light absorption and suppressed charge recombination...
July 20, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28726938/design-and-characterization-of-a-plasmonic-doppler-grating-for-azimuthal-angle-resolved-surface-plasmon-resonances
#19
Kel-Meng See, Fan-Cheng Lin, Jer-Shing Huang
We present a two-dimensional plasmonic Doppler grating (PDG) for broadband and azimuthal angle-resolved nanophotonic applications. The PDG consists of a set of non-concentric circular rings mimicking the wavefronts of a moving point source that exhibits the Doppler effect and thereby offers a continuous azimuthal angle-dependent lattice momentum for photon-plasmon coupling. The center and span of the working frequency window are fully designable for optimal performance in specific applications. We detail the design, fabrication and optical characterization of the PDG...
July 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28726784/binding-of-doxorubicin-to-sorcin-impairs-cell-death-and-increases-drug-resistance-in-cancer-cells
#20
Ilaria Genovese, Annarita Fiorillo, Andrea Ilari, Silvia Masciarelli, Francesco Fazi, Gianni Colotti
Sorcin is a calcium binding protein that plays an important role in multidrug resistance (MDR) in tumors, since its expression confers resistance to doxorubicin and to other chemotherapeutic drugs. In this study, we show that Sorcin is able to bind doxorubicin, vincristine, paclitaxel and cisplatin directly and with high affinity. The high affinity binding of doxorubicin to sorcin has been demonstrated with different techniques, that is, surface plasmon resonance, fluorescence titration and X-ray diffraction...
July 20, 2017: Cell Death & Disease
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