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https://www.readbyqxmd.com/read/28449373/biophysical-analysis-of-the-dynamics-of-calmodulin-interactions-with-neurogranin-and-ca2-calmodulin-dependent-kinase-ii
#1
Christian Seeger, Vladimir O Talibov, U Helena Danielson
Calmodulin (CaM) functions depend on interactions with CaM-binding proteins, regulated by Ca2+. Induced structural changes influence the affinity, kinetics, and specificities of the interactions. The dynamics of CaM interactions with neurogranin (Ng) and the CaM-binding region of Ca2+/calmodulin-dependent kinase II (CaMKII290-309 ) have been studied using biophysical methods. These proteins have opposite Ca2+ dependencies for CaM binding. Surface plasmon resonance biosensor analysis confirmed that Ca2+ and CaM interact very rapidly, and with moderate affinity ( KDSPR=3μM)...
April 27, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/28448875/bioengineered-mannan-sulphate-capped-silver-nanoparticles-for-accelerated-and-targeted-wound-healing-physicochemical-and-biological-investigations
#2
Megha Mugade, Milind Patole, Varsha Pokharkar
In the present study, surface functionalized mannan sulphate silver nanoparticles (MS-AgNPs) were prepared and assessed for their wound healing potential. 20nm sized, spherical MS-AgNPs were prepared by one pot synthesis approach wherein the sulphated polysaccharide mannan sulphate (MS) played dual role of reducing as well as capping agent. The crystalline MS-AgNPs exhibited surface plasmon resonance centered at 400nm along with -32.40mV zeta potential. These stable MS-AgNPs showed enhanced cytocompatibility, targeting potential and cellular uptake in murine macrophages, human skin fibroblasts and human keratinocytes as compared to citrate reduced silver nanoparticles (C-AgNPs)...
April 24, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28448040/determination-of-high-affinity-antibody-antigen-binding-kinetics-using-four-biosensor-platforms
#3
Danlin Yang, Ajit Singh, Helen Wu, Rachel Kroe-Barrett
Label-free optical biosensors are powerful tools in drug discovery for the characterization of biomolecular interactions. In this study, we describe the use of four routinely used biosensor platforms in our laboratory to evaluate the binding affinity and kinetics of ten high-affinity monoclonal antibodies (mAbs) against human proprotein convertase subtilisin kexin type 9 (PCSK9). While both Biacore T100 and ProteOn XPR36 are derived from the well-established Surface Plasmon Resonance (SPR) technology, the former has four flow cells connected by serial flow configuration, whereas the latter presents 36 reaction spots in parallel through an improvised 6 x 6 crisscross microfluidic channel configuration...
April 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447977/plasmonic-trapping-and-release-of-nanoparticles-in-a-monitoring-environment
#4
Jung-Dae Kim, Yong-Gu Lee
Plasmonic tweezers use surface plasmon polaritons to confine polarizable nanoscale objects. Among the various designs of plasmonic tweezers, only a few can observe immobilized particles. Moreover, a limited number of studies have experimentally measured the exertable forces on the particles. The designs can be classified as the protruding nanodisk type or the suppressed nanohole type. For the latter, microscopic observation is extremely challenging. In this paper, a new plasmonic tweezer system is introduced to monitor particles, both in directions parallel and orthogonal to the symmetric axis of a plasmonic nanohole structure...
April 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447698/anisotropic-metal-nanoparticles-for-surface-enhanced-raman-scattering
#5
Javier Reguera, Judith Langer, Dorleta Jiménez de Aberasturi, Luis M Liz-Marzán
The optimization of the enhancement of Raman scattering by plasmonic effects is largely determined by the properties of the enhancing substrates. The main parameters behind this effect are related to the morphology of plasmonic nanoparticles and their relative distribution within the substrate. We focus this tutorial review on the effects of nanoparticle morphology, for the particular case of anisotropic metal nanoparticles. Anisotropy in silver and gold nanoparticles offers the possibility to tailor their plasmonic properties and intrinsic electromagnetic "hotspots"...
April 27, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28447694/formation-of-bimetallic-dumbbell-shaped-particles-with-a-hollow-junction-during-galvanic-replacement-reaction
#6
Sravan Thota, Yadong Zhou, Shutang Chen, Shengli Zou, Jing Zhao
The galvanic replacement reaction (GRR) has been shown to be an effective method to fine tune the structure of monometallic nanoparticles by controlling the precursor concentration and surface ligands. However, the structural evolution of nanoparticles is not well understood in multimetallic systems, where along with oxidation, dealloying and diffusion occur simultaneously. Here, we demonstrate that by controlling the rate of GRR in AuCu alloy nanorods, they can be transformed into either AuCu hollow rods or AuCu@Au core-shell spheroids...
April 27, 2017: Nanoscale
https://www.readbyqxmd.com/read/28446794/up-scalable-full-colour-plasmonic-pixels-with-controllable-hue-brightness-and-saturation
#7
Renilkumar Mudachathi, Takuo Tanaka
It has long been the interests of scientists to develop ink free colour printing technique using nano structured materials inspired by brilliant colours found in many creatures like butterflies and peacocks. Recently isolated metal nano structures exhibiting preferential light absorption and scattering have been explored as a promising candidate for this emerging field. Applying such structures in practical use, however, demands the production of individual colours with distinct reflective peaks, tunable across the visible wavelength region combined with controllable colour attributes and economically feasible fabrication...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28446792/sulfated-hyaluronan-derivatives-modulate-tgf-%C3%AE-1-receptor-complex-formation-possible-consequences-for-tgf-%C3%AE-1-signaling
#8
Linda Koehler, Sergey Samsonov, Sandra Rother, Sarah Vogel, Sebastian Köhling, Stephanie Moeller, Matthias Schnabelrauch, Jörg Rademann, Ute Hempel, M Teresa Pisabarro, Dieter Scharnweber, Vera Hintze
Glycosaminoglycans are known to bind biological mediators thereby modulating their biological activity. Sulfated hyaluronans (sHA) were reported to strongly interact with transforming growth factor (TGF)-β1 leading to impaired bioactivity in fibroblasts. The underlying mechanism is not fully elucidated yet. Examining the interaction of all components of the TGF-β1:receptor complex with sHA by surface plasmon resonance, we could show that highly sulfated HA (sHA3) blocks binding of TGF-β1 to its TGF-β receptor-I (TβR-I) and -II (TβR-II)...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28445997/using-metal-multilayer-dielectric-structure-to-increase-sensitivity-of-surface-plasmon-resonance-sensor
#9
Svitlana G Ilchenko, Ruslan A Lymarenko, Victor B Taranenko
We propose using a specially designed metal-multilayer-dielectric structure deposited on glass substrate to enhance the evanescent field and improve the sensitivity of the surface plasmon resonance sensor. The proposed structure supports both hybrid plasmonic transverse magnetic modes and conventional waveguide transverse electric modes. We show numerically the significant enhancement of the evanescent field and improvement of the sensitivity for the waveguide transverse electric mode.
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28445644/broadband-fluorescence-enhancement-with-self-assembled-silver-nanoparticle-optical-antennas
#10
Carolin Vietz, Izabela Kaminska, Maria Sanz Paz, Philip Tinnefeld, Guillermo P Acuna
Plasmonic structures are known to affect the fluorescence properties of dyes placed in close proximity. This effect has been exploited in combination with single-molecule techniques for several applications in the field of biosensing. Among these plasmonic structures, top-down zero mode waveguides stand out due to their broadband capabilities. In contrast, optical antennas based on gold nanostructures exhibit fluorescence enhancement on a narrow fraction of the visible spectrum typically restricted to the red to near-infrared region...
April 26, 2017: ACS Nano
https://www.readbyqxmd.com/read/28445622/thiocarbohydrates-on-gold-nanoparticles-strong-influences-of-stereocenters-on-binding-affinitiy-and-interparticle-forces
#11
Torsten Linker, Prashant Pavashe, Werner Fudickar
Carbohydrates carrying thiol groups at the C-2 position have been attached to gold nanoparticles (AuNP) with stereocenters in close proximity to the surface for the first time. Their configurations can be clearly distinguished by the tendency of particle aggregation. AuNP surface plasmon resonance (SPR), x-ray photoelectron spectroscopy (XPS), and IR spectroscopy indicate that the thiocarbohydrates replace citrate molecules at different rates causing aggregation and eventually precipitation. A quantitative formulation of this aggregation process shows that reactivities can vary by several magnitudes...
April 26, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28445133/baicalein-decreases-uric-acid-and-prevents-hyperuricemic-nephropathy-in-mice
#12
Xiaolu Meng, Zhuo Mao, Xin Li, Dandan Zhong, Min Li, Yingli Jia, Jing Wei, Baoxue Yang, Hong Zhou
Baicalein, a natural flavonoid, is structurally advantageous for binding to xanthine oxidoreductase. In our study, molecular docking analysis and Surface Plasmon Resonance revealed a direct interaction between baicalein and xanthine oxidoreductase. Moreover, 50 mg/kg/d baicalein treatment significantly suppressed the viability of xanthine oxidoreductase in hyperuricemia mouse model. The data showed that baicalein remarkably prevented renal dysfunction, ameliorated kidney fibrosis, alleviated epithelial-mesenchymal transition and oxidative stress in hyperuricemia mice...
April 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28443939/magnetic-fano-resonance-induced-second-harmonic-generation-enhancement-in-plasmonic-metamolecule-rings
#13
Da-Jie Yang, Song-Jin Im, Gui-Ming Pan, Si-Jing Ding, Zhong-Jian Yang, Zhong-Hua Hao, Li Zhou, Qu-Quan Wang
The "artificial magnetic" resonance in plasmonic metamolecules extends the potential application of magnetic resonance from terahertz to optical frequency bypassing the problem of magnetic response saturation by replacing the conduction current with the ring displacement current. So far, the magnetic Fano resonance-induced nonlinearity enhancement in plasmonic metamolecule rings has not been reported. Here, we use the magnetic Fano resonance to enhance second-harmonic generation (SHG) in plasmonic metamolecule rings...
April 26, 2017: Nanoscale
https://www.readbyqxmd.com/read/28443911/fabrication-of-plasmonic-au-pd-alloy-nanoparticles-for-photocatalytic-suzuki-miyaura-reactions-under-ambient-conditions
#14
Dongxu Han, Zongbi Bao, Huabin Xing, Yiwen Yang, Qilong Ren, Zhiguo Zhang
The Suzuki-Miyaura reaction is one of the most popular and efficient routes for the formation of carbon-carbon bonds in both laboratorial and industrial synthetic processes. Here, we report for the first time that a Au-Pd/TiO2 catalyst could be utilized efficiently for Suzuki-Miyaura reactions at ambient conditions under visible light with high activity and reusability. Mechanistic investigation by means of experimental methods revealed that through the strong local surface plasma resonance of Au under the visible light, the hot electrons of Au could be injected into Pd in the bimetallic nanoparticles, thus facilitating activation of the aryl halides...
April 26, 2017: Nanoscale
https://www.readbyqxmd.com/read/28443908/polyvalent-c-glycomimetics-based-on-l-fucose-or-d-mannose-as-potent-dc-sign-antagonists
#15
Benedetta Bertolotti, Ieva Sutkeviciute, Martino Ambrosini, Renato Ribeiro-Viana, Javier Rojo, Franck Fieschi, Hana Dvořáková, Martina Kašáková, Kamil Parkan, Martina Hlaváčková, Kateřina Nováková, Jitka Moravcová
The C-type lectin DC-SIGN expressed on immature dendritic cells is a promising target for antiviral drug development. Previously, we have demonstrated that mono- and divalent C-glycosides based on d-manno and l-fuco configurations are promising DC-SIGN ligands. Here, we described the convergent synthesis of C-glycoside dendrimers decorated with 4, 6, 9, and 12 α-l-fucopyranosyl units and with 9 and 12 α-d-mannopyranosyl units. Their affinity against DC-SIGN was assessed by surface plasmon resonance (SPR) assays...
April 26, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28443837/real-time-plasmonic-qpcr-how-fast-is-ultra-fast-30-cycles-in-54-seconds
#16
Philip J R Roche, Mohamed Najih, Seung S Lee, Lenore K Beitel, Matthew L Carnevale, Miltiadis Paliouras, Andrew G Kirk, Mark A Trifiro
Polymerase Chain Reaction (PCR) is a critical tool for biological research investigators but recently it also has been making a significant impact in clinical, veterinary and agricultural applications. Plasmonic PCR, which employs the very efficient heat transfer of optically irradiated metallic nanoparticles, is a simple and powerful methodology to drive PCR reactions. The scalability of next generation plasmonic PCR technology will introduce various forms of PCR applications ranging from small footprint portable point of care diagnostic devices to large footprint central laboratory multiplexing devices...
April 26, 2017: Analyst
https://www.readbyqxmd.com/read/28442721/near-field-surface-plasmons-on-quasicrystal-metasurfaces
#17
Quanlong Yang, Xueqian Zhang, Shaoxian Li, Quan Xu, Ranjan Singh, Yongmin Liu, Yanfeng Li, Sergey S Kruk, Jianqiang Gu, Jiaguang Han, Weili Zhang
Excitation and manipulation of surface plasmons (SPs) are essential in developing cutting-edge plasmonic devices for medical diagnostics, biochemical spectroscopy and communications. The most common approach involves designing an array of periodic slits or grating apertures that enables coupling of the incident light to the SP modes. In recent years, plasmonic resonances, including extraordinary optical transmission through periodic arrays, quasicrystals and random aperture arrays, have been investigated in the free space...
December 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28442575/structure-and-energetics-of-pairwise-interactions-between-proteasome-subunits-rpn2-rpn13-and-ubiquitin-clarify-a-substrate-recruitment-mechanism
#18
Ryan T VanderLinden, Casey W Hemmis, Tingting Yao, Howard Robinson, Christopher P Hill
The 26S proteasome is a large cellular assembly that mediates the selective degradation of proteins in the nucleus and cytosol and is an established target for anti-cancer therapeutics. Protein substrates are typically targeted to the proteasome through modification with a polyubiquitin chain, which can be recognized by several proteasome-associated ubiquitin receptors. One of these receptors, RPN13/ADRM1, is recruited to the proteasome through direct interaction with the large scaffolding protein RPN2 within the 19S regulatory particle...
April 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28441500/thermoplasmonic-semitransparent-nanohole-electrodes
#19
Daniel Tordera, Dan Zhao, Anton V Volkov, Xavier Crispin, Magnus P Jonsson
Non-radiative decay of plasmons in metallic nanostructures offers unique means for light-to-heat conversion at the nanoscale. Typical thermoplasmonic systems utilize discrete particles, while metal nanohole arrays were instead considered suitable as heat sinks to reduce heating effects. By contrast, we show for the first time that, under uniform broadband illumination (e.g. the sun), ultrathin plasmonic nanohole arrays can be highly competitive plasmonic heaters and provide significantly higher temperatures than analogous nanodisk arrays...
April 25, 2017: Nano Letters
https://www.readbyqxmd.com/read/28441499/low-loss-plasmonic-dielectric-nanoresonators
#20
Yi Yang, Owen Miller, Thomas Christensen, John D Joannopoulos, Marin Soljacic
Material losses in metals are a central bottleneck in plasmonics for many applications. Here we propose and theoretically demonstrate that metal losses can be successfully mitigated with dielectric particles on metallic films, giving rise to hybrid dielectric--metal resonances. In the far field, they yield strong and efficient scattering, beyond even the theoretical limits of all-metal and all-dielectric structures. In the near field, they offer high-Purcell-factor (${>}5000$), high-quantum-efficiency (${>}90\%$), and highly directional emission at visible and infrared wavelengths...
April 25, 2017: Nano Letters
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