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Lymphatic endothelial cells

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https://www.readbyqxmd.com/read/28530642/rasa1-regulates-the-function-of-lymphatic-vessel-valves-in-mice
#1
Philip E Lapinski, Beth A Lubeck, Di Chen, Abbas Doosti, Scott D Zawieja, Michael J Davis, Philip D King
Capillary malformation-arteriovenous malformation (CM-AVM) is a blood and lymphatic vessel (LV) disorder that is caused by inherited inactivating mutations of the RASA1 gene, which encodes p120 RasGAP (RASA1), a negative regulator of the Ras small GTP-binding protein. How RASA1 mutations lead to the LV leakage defects that occur in CM-AVM is not understood. Here, we report that disruption of the Rasa1 gene in adult mice resulted in loss of LV endothelial cells (LECs) specifically from the leaflets of intraluminal valves in collecting LVs...
May 22, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28526302/an-important-role-of-vegf-c-in-promoting-lymphedema-development
#2
Epameinondas Gousopoulos, Steven T Proulx, Samia B Bachmann, Lothar C Dieterich, Jeannette Scholl, Sinem Karaman, Roberta Bianchi, Michael Detmar
Secondary lymphedema is a common post-cancer treatment complication but the pathomechanisms underlying the disease remain unclear. Using a mouse-tail lymphedema model, we found an increase in local and systemic levels of the lymphangiogenic factor VEGF-C, and identified CD68+ macrophages as a cellular source. Surprisingly, overexpression of VEGF-C in a transgenic mouse model led to aggravation of lymphedema with increased immune cell infiltration and vascular leakage in comparison to wild-type littermates. Conversely, blockage of VEGF-C by overexpression of soluble VEGFR3 reduced edema development, diminishing inflammation and blood vascular leakage...
May 16, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28526034/differential-darc-ackr1-expression-distinguishes-venular-from-non-venular-endothelial-cells-in-murine-tissues
#3
Aude Thiriot, Carolina Perdomo, Guiying Cheng, Igor Novitzky-Basso, Sara McArdle, Jamie K Kishimoto, Olga Barreiro, Irina Mazo, Robinson Triboulet, Klaus Ley, Antal Rot, Ulrich H von Andrian
BACKGROUND: Intravascular leukocyte recruitment in most vertebrate tissues is restricted to postcapillary and collecting venules, whereas capillaries and arterioles usually support little or no leukocyte adhesion. This segmental restriction is thought to be mediated by endothelial, rather than hemodynamic, differences. The underlying mechanisms are largely unknown, in part because effective tools to distinguish, isolate, and analyze venular endothelial cells (V-ECs) and non-venular endothelial cells (NV-ECs) have been unavailable...
May 19, 2017: BMC Biology
https://www.readbyqxmd.com/read/28508272/nasopharyngeal-angiofibroma-a-clinical-histopathological-and-immunohistochemical-study-of-42-cases-with-emphasis-on-stromal-features
#4
Celeste Sánchez-Romero, Roman Carlos, Juan Pablo Díaz Molina, Lester D R Thompson, Oslei Paes de Almeida, Alicia Rumayor Piña
Nasopharyngeal angiofibroma is a benign but aggressive tumor of unknown etiology, typically occurring in adolescent males. It is described as a rare neoplasm; however, the prevalence seems to have geographic differences. All cases referred to our head and neck clinical and pathology service were reviewed. Most of the patients presented at an advanced stage. The clinical and radiographic features are presented and discussed. Histologically, the tumor shows a highly vascular fibrous proliferation with characteristic plump, angulated and stellate cells, categorized as fibroblasts...
May 15, 2017: Head and Neck Pathology
https://www.readbyqxmd.com/read/28504698/dendritic-cells-enter-lymph-vessels-by-hyaluronan-mediated-docking-to-the-endothelial-receptor-lyve-1
#5
Louise A Johnson, Suneale Banerji, William Lawrance, Uzi Gileadi, Gennaro Prota, Kayla A Holder, Yaowaluck M Roshorm, Tomáš Hanke, Vincenzo Cerundolo, Nicholas W Gale, David G Jackson
Trafficking of tissue dendritic cells (DCs) via lymph is critical for the generation of cellular immune responses in draining lymph nodes (LNs). In the current study we found that DCs docked to the basolateral surface of lymphatic vessels and transited to the lumen through hyaluronan-mediated interactions with the lymph-specific endothelial receptor LYVE-1, in dynamic transmigratory-cup-like structures. Furthermore, we show that targeted deletion of the gene Lyve1, antibody blockade or depletion of the DC hyaluronan coat not only delayed lymphatic trafficking of dermal DCs but also blunted their capacity to prime CD8(+) T cell responses in skin-draining LNs...
May 15, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28498105/intracellular-uptake-of-macromolecules-by-brain-lymphatic-endothelial-cells-during-zebrafish-embryonic-development
#6
Max van Lessen, Shannon Shibata-Germanos, Andreas van Impel, Thomas A Hawkins, Jason Rihel, Stefan Schulte-Merker
The lymphatic system controls fluid homeostasis and the clearance of macromolecules from interstitial compartments. In mammals brain lymphatics were only recently discovered, with significant implications for physiology and disease. We examined zebrafish for the presence of brain lymphatics and found loosely connected endothelial cells with lymphatic molecular signature covering parts of the brain without forming endothelial tubular structures. These brain lymphatic endothelial cells (BLECs) derive from venous endothelium, are distinct from macrophages, and are sensitive to loss of Vegfc...
May 12, 2017: ELife
https://www.readbyqxmd.com/read/28498096/characterization-of-the-anti-bovine-podoplanin-monoclonal-antibody-pmab-44
#7
Shinji Yamada, Ryusuke Honma, Mika K Kaneko, Takuro Nakamura, Miyuki Yanaka, Noriko Saidoh, Michiaki Takagi, Satoru Konnai, Yukinari Kato
A type I transmembrane sialoglycoprotein podoplanin (PDPN) is expressed in several normal cells, including podocytes of the kidney, type I alveolar cells of the lung, and lymphatic endothelial cells. We recently produced an anti-bovine PDPN (bovPDPN) monoclonal antibody (mAb), PMab-44, by immunizing mice with recombinant proteins of bovPDPN. In this study, we determined the critical epitope of PMab-44 for the recognition of bovPDPN using many deletion mutants and point mutants of bovPDPN. Flow cytometric analyses revealed that the epitope of PMab-44 was Glu46-Thr50, which corresponds to platelet aggregation-stimulating (PLAG) domain-3...
May 12, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28490876/one-pot-synthesis-of-dextran-coated-iron-oxide-nanoclusters-for-real-time-regional-lymph-node-mapping
#8
Chaoping Fu, Haipeng Zhou, Yanan Wang, Dong Liu, Junmeng Li, Haijun Deng, Xiaolong Qi, Tao Chen, Li-Ming Zhang, Guoxin Li
The intraoperative precision cleaning of lymph nodes (LNs) is an essential component of treating neoplastic disease. To develop efficient probes for the targeted detection of LNs that could act as carriers for the specific diagnosis and treatment of metastatic LNs in the future, dextran-coated iron oxide nanoclusters (DIONs) were synthesized using a one-pot coprecipitation procedure. These modified DIONs have good water dispersibility, cytocompatibility, an optimum size, and a stable, dark brown color for LN imaging...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28490670/leukotriene-b4-antagonism-ameliorates-experimental-lymphedema
#9
Wen Tian, Stanley G Rockson, Xinguo Jiang, Jeanna Kim, Adrian Begaye, Eric M Shuffle, Allen B Tu, Matthew Cribb, Zhanna Nepiyushchikh, Abdullah H Feroze, Roham T Zamanian, Gundeep S Dhillon, Norbert F Voelkel, Marc Peters-Golden, Jan Kitajewski, J Brandon Dixon, Mark R Nicolls
Acquired lymphedema is a cancer sequela and a global health problem currently lacking pharmacologic therapy. We have previously demonstrated that ketoprofen, an anti-inflammatory agent with dual 5-lipoxygenase and cyclooxygenase inhibitory properties, effectively reverses histopathology in experimental lymphedema. We show that the therapeutic benefit of ketoprofen is specifically attributable to its inhibition of the 5-lipoxygenase metabolite leukotriene B4 (LTB4). LTB4 antagonism reversed edema, improved lymphatic function, and restored lymphatic architecture in the murine tail model of lymphedema...
May 10, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28487567/a-robust-in-vitro-model-for-trans-lymphatic-endothelial-migration
#10
Yanbao Xiong, C Colin Brinkman, Konrad S Famulski, Emmanuel F Mongodin, Colin J Lord, Keli L Hippen, Bruce R Blazar, Jonathan S Bromberg
Trans-endothelial migration (TEM) is essential for leukocyte circulation. While much is known about trans-blood endothelial migration, far less is known about trans-lymphatic endothelial migration. We established an in vitro system to evaluate lymphatic TEM for various cell types across primary mouse and human lymphatic endothelial cells (LEC), and validated the model for the murine LEC cell line SVEC4-10. T cells exhibited enhanced unidirectional migration from the basal (abluminal) to the apical (luminal) surface across LEC, whereas for blood endothelial cells (BEC) they migrated similarly in both directions...
May 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486010/lymphatic-endothelial-cells-under-mechanical-stress-altered-expression-of-inflammatory-cytokines-and-fibrosis
#11
Sheri Wang, Daibang Nie, J Peter Rubin, Lauren Kokai
BACKGROUND: Secondary lymphedema, resulting from damage to lymphatic vessels, is a common sequela following surgical removal of lymph nodes for cancer. Current therapeutics for treating lymphedema are limited and further research on underlying causes is warranted. Published studies on molecular mechanisms of lymphedema primarily focus on lymphatic endothelial cells (LECs), which comprise the innermost lining of lymphatic capillaries and collecting vessels. However, traditional static culture of LECs may not adequately recapitulate the lymphedemous cell phenotype as transcriptomal comparison of human dermal LECs has shown significant differences in ex vivo and in vitro LEC gene expression...
May 9, 2017: Lymphatic Research and Biology
https://www.readbyqxmd.com/read/28481947/brugia-malayi-microfilariae-adhere-to-human-vascular-endothelial-cells-in-a-c3-dependent-manner
#12
Jan-Hendrik Schroeder, David McCarthy, Tadge Szestak, Darren A Cook, Mark J Taylor, Alister G Craig, Charlotte Lawson, Rachel A Lawrence
Brugia malayi causes the human tropical disease, lymphatic filariasis. Microfilariae (Mf) of this nematode live in the bloodstream and are ingested by a feeding mosquito vector. Interestingly, in a remarkable co-evolutionary adaptation, Mf appearance in the peripheral blood follows a circadian periodicity and reaches a peak when the mosquito is most likely to feed. For the remaining hours, the majority of Mf sequester in the lung capillaries. This circadian phenomenon has been widely reported and is likely to maximise parasite fitness and optimise transmission potential...
May 8, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28475514/recurrent-cutaneous-angiosarcoma-of-the-scalp-with-aberrant-expression-of-s100-a-case-report
#13
Laura Macías-García, Concepción Lara-Bohorquez, Enrique Jorquera-Barquero, Juan J Ríos-Martín
Angiosarcoma (AS) is a malignant mesenchymal neoplasm of endothelial origin with a predominantly lymphatic immunophenotype, which accounts for less than 1% of all sarcomas. Cutaneous AS of the scalp is associated with high rates of local recurrence and a poor prognosis. Histologically, poorly differentiated AS often comprises solid epithelioid cells, although rare variants involving spindle cells have been reported; diagnosis requires immunohistochemical analysis using vascular cell markers. We report on a cutaneous spindle-cell AS of the scalp in a female patient; key features included spontaneous regression after biopsy, local recurrence 2 years later, and aberrant nuclear staining for S100 protein in an area of the tumor not expressing CD34 or D2-40...
April 25, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28467980/distinct-distribution-of-immunocytes-in-a-retropharyngeal-lymphadenopathy-associated-with-kawasaki-disease-a-case-study-compared-with-tonsillitis
#14
Seigo Okada, Yuki Kobayashi-Fujiwara, Atsunori Oga, Takashi Furuta, Kenzo Ikemoto, Hironori Fujii, Yasufumi Sakata, Yasuo Suzuki, Shunji Hasegawa, Takeshi Kusuda, Hiroshi Itoh, Hiroshi Yamashita, Shouichi Ohga
OBJECTIVES: Retropharyngeal lesions are often associated with Kawasaki disease (KD). A 4-year-old male first presented a peritonsillar and retropharyngeal abscess-like lesion. Surgical tonsillectomy was performed to avoid a risk of mediastinal abscess, but he fulfilled the diagnostic criteria of KD after the operation. This prompted us to perform a histological study on the KD tonsils. METHODS: The histopathology of the KD tonsil specimens were compared with hypertrophic tonsils obtained from 4 patients with chronic tonsillitis unrelated to KD assessed by the immunostainings...
May 4, 2017: Cardiology
https://www.readbyqxmd.com/read/28467903/locally-triggered-release-of-the-chemokine-ccl21-promotes-dendritic-cell-transmigration-across-lymphatic-endothelia
#15
Kari Vaahtomeri, Markus Brown, Robert Hauschild, Ingrid De Vries, Alexander Franz Leithner, Matthias Mehling, Walter Anton Kaufmann, Michael Sixt
Trafficking cells frequently transmigrate through epithelial and endothelial monolayers. How monolayers cooperate with the penetrating cells to support their transit is poorly understood. We studied dendritic cell (DC) entry into lymphatic capillaries as a model system for transendothelial migration. We find that the chemokine CCL21, which is the decisive guidance cue for intravasation, mainly localizes in the trans-Golgi network and intracellular vesicles of lymphatic endothelial cells. Upon DC transmigration, these Golgi deposits disperse and CCL21 becomes extracellularly enriched at the sites of endothelial cell-cell junctions...
May 2, 2017: Cell Reports
https://www.readbyqxmd.com/read/28467822/fgf-dependent-metabolic-control-of-vascular-development
#16
Pengchun Yu, Kerstin Wilhelm, Alexandre Dubrac, Joe K Tung, Tiago C Alves, Jennifer S Fang, Yi Xie, Jie Zhu, Zehua Chen, Frederik De Smet, Jiasheng Zhang, Suk-Won Jin, Lele Sun, Hongye Sun, Richard G Kibbey, Karen K Hirschi, Nissim Hay, Peter Carmeliet, Thomas W Chittenden, Anne Eichmann, Michael Potente, Michael Simons
Blood and lymphatic vasculatures are intimately involved in tissue oxygenation and fluid homeostasis maintenance. Assembly of these vascular networks involves sprouting, migration and proliferation of endothelial cells. Recent studies have suggested that changes in cellular metabolism are important to these processes. Although much is known about vascular endothelial growth factor (VEGF)-dependent regulation of vascular development and metabolism, little is understood about the role of fibroblast growth factors (FGFs) in this context...
May 11, 2017: Nature
https://www.readbyqxmd.com/read/28459441/mural-lymphatic-endothelial-cells-regulate-meningeal-angiogenesis-in-the-zebrafish
#17
Neil I Bower, Katarzyna Koltowska, Cathy Pichol-Thievend, Isaac Virshup, Scott Paterson, Anne K Lagendijk, Weili Wang, Benjamin W Lindsey, Stephen J Bent, Sungmin Baek, Maria Rondon-Galeano, Daniel G Hurley, Naoki Mochizuki, Cas Simons, Mathias Francois, Christine A Wells, Jan Kaslin, Benjamin M Hogan
Mural cells of the vertebrate brain maintain vascular integrity and function, play roles in stroke and are involved in maintenance of neural stem cells. However, the origins, diversity and roles of mural cells remain to be fully understood. Using transgenic zebrafish, we identified a population of isolated mural lymphatic endothelial cells surrounding meningeal blood vessels. These meningeal mural lymphatic endothelial cells (muLECs) express lymphatic endothelial cell markers and form by sprouting from blood vessels...
May 1, 2017: Nature Neuroscience
https://www.readbyqxmd.com/read/28459049/engineering-blood-and-lymphatic-microvascular-networks-in-fibrin-matrices
#18
Lea Knezevic, Mira Schaupper, Severin Mühleder, Katharina Schimek, Tobias Hasenberg, Uwe Marx, Eleni Priglinger, Heinz Redl, Wolfgang Holnthoner
Vascular network engineering is essential for nutrient delivery to tissue-engineered constructs and, consequently, their survival. In addition, the functionality of tissues also depends on tissue drainage and immune cell accessibility, which are the main functions of the lymphatic system. Engineering both the blood and lymphatic microvasculature would advance the survival and functionality of tissue-engineered constructs. The aim of this study was to isolate pure populations of lymphatic endothelial cells (LEC) and blood vascular endothelial cells (BEC) from human dermal microvascular endothelial cells and to study their network formation in our previously described coculture model with adipose-derived stromal cells (ASC) in fibrin scaffolds...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/28448484/tissue-engineered-3d-human-lymphatic-microvascular-network-for-in-vitro-studies-of-lymphangiogenesis
#19
Laure Gibot, Todd Galbraith, Jennifer Bourland, Anita Rogic, Mihaela Skobe, François A Auger
This protocol describes a unique in vitro method for the generation of a 3D human lymphatic network within native connective tissue devoid of any exogenous material such as scaffolds or growth factors. In this five-stage protocol, human lymphatic endothelial cells (LECs) cocultured with dermal fibroblasts spontaneously organize into a stable 3D lymphatic capillary network. Stage 1 involves the isolation of primary fibroblasts and LECs from human skin. Fibroblasts are then cultured to produce connective tissue rich in extracellular matrix (stage 2), onto which LECs are seeded to form a network (stage 3)...
May 2017: Nature Protocols
https://www.readbyqxmd.com/read/28448013/precision-cut-mouse-lung-slices-to-visualize-live-pulmonary-dendritic-cells
#20
Miranda R Lyons-Cohen, Seddon Y Thomas, Donald N Cook, Hideki Nakano
Inhalation of allergens and pathogens elicits multiple changes in a variety of immune cell types in the lung. Flow cytometry is a powerful technique for quantitative analysis of cell surface proteins on immune cells, but it provides no information on the localization and migration patterns of these cells within the lung. Similarly, chemotaxis assays can be performed to study the potential of cells to respond to chemotactic factors in vitro, but these assays do not reproduce the complex environment of the intact lung...
April 5, 2017: Journal of Visualized Experiments: JoVE
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