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https://www.readbyqxmd.com/read/29161183/epitope-mapping-of-monoclonal-antibody-pmab-38-against-dog-podoplanin
#1
Yao-Wen Chang, Shinji Yamada, Mika K Kaneko, Yukinari Kato
Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is extensively expressed by normal lymphatic endothelial cells, renal podocytes, and pulmonary type I alveolar cells. Nevertheless, increased expression of PDPN in malignant tumors not only associates with poor prognosis but also facilitates hematogenous metastasis through interaction with C-type lectin-like receptor-2 presented on platelets, followed by PDPN-mediated platelet activation. We previously reported a novel PMab-38 antibody, an anti-dog PDPN (dPDPN) monoclonal antibody, which specifically recognizes PDPN in squamous cell carcinomas melanomas and cancer-associated fibroblasts in canine cancer tissues...
November 21, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29158788/programmed-death-ligand-1-pd-l1-expression-in-primary-angiosarcoma
#2
Gerardo Botti, Giosuè Scognamiglio, Laura Marra, Antonio Pizzolorusso, Maurizio Di Bonito, Rossella De Cecio, Monica Cantile, Annarosaria De Chiara
Angiosarcomas are rare malignant endothelial-cell tumors of vascular or lymphatic origin, and are among the most aggressive subtypes of soft-tissue sarcomas. The prognosis is poor and treatment is challenging in many cases. PD-1/PD-L1 pathway plays a critical role in immune escape of tumor cells. Recent studies described that PD-L1 is widely expressed in various types of cancer, providing the basis for the development of PD1/PD-L1 antibodies as anti-cancer immunotherapy. Despite the well-known potential of PD-L1 as prognostic and predictive biomarker, only few studies described its IHC expression in cancer subtypes for the extreme difficulty in developing standard protocol with the different antibody clones available...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29151910/role-and-importance-of-the-expression-of-transcription-factor-foxc2-in-cervical-cancer
#3
Jing Wang, Xiujuan Yue
The aim of the present study was to investigate the relationship between the expression of transcription factor forkhead box C2 (FOXC2) and the clinical features of cervical cancer. A total of 66 patients with cervical cancer, 42 patients with cervical intraepithelial neoplasia (CIN) and 25 patients with cervical inflammation were enrolled. The positive expression rates and expression levels of mRNA of FOXC2, E-cadherin, N-cadherin, vascular endothelial growth factor (VEGF), stromal cell-derived factor-1 (SDF-1), Notch protein and lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) in cervical tissues were detected using immunohistochemistry and RT-PCR...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29141865/development-and-plasticity-of-meningeal-lymphatic-vessels
#4
Salli Antila, Sinem Karaman, Harri Nurmi, Mikko Airavaara, Merja H Voutilainen, Thomas Mathivet, Dmitri Chilov, Zhilin Li, Tapani Koppinen, Jun-Hee Park, Shentong Fang, Aleksanteri Aspelund, Mart Saarma, Anne Eichmann, Jean-Léon Thomas, Kari Alitalo
The recent discovery of meningeal lymphatic vessels (LVs) has raised interest in their possible involvement in neuropathological processes, yet little is known about their development or maintenance. We show here that meningeal LVs develop postnatally, appearing first around the foramina in the basal parts of the skull and spinal canal, sprouting along the blood vessels and cranial and spinal nerves to various parts of the meninges surrounding the central nervous system (CNS). VEGF-C, expressed mainly in vascular smooth muscle cells, and VEGFR3 in lymphatic endothelial cells were essential for their development, whereas VEGF-D deletion had no effect...
November 15, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29138199/electrosclerotherapy-for-capillary-malformations-study-protocol-for-a-randomised-within-patient-controlled-pilot-trial
#5
Sophie E R Horbach, Albert Wolkerstorfer, Daniel Martijn de Bruin, Chantal M A M van der Horst
INTRODUCTION: The current state-of-the-art treatment modality for hypertrophic capillary malformations (CMs), laser therapy, has a considerable rate of non-responders and recurrence. Intralesional bleomycin injections (or 'sclerotherapy') are commonly used to treat venous and lymphatic malformations with an excellent effect, but these intravascular injections are not possible in CMs due to the small diameter of the vessels. Electroporation-an electric field applied to the tissue-could increase the permeability of endothelial cells, which could theoretically facilitate targeted localised bleomycin delivery...
November 14, 2017: BMJ Open
https://www.readbyqxmd.com/read/29133178/lymphatic-vasculature-in-human-dural-superior-sagittal-sinus-implications-for-neurodegenerative-proteinopathies
#6
Naomi P Visanji, Anthony E Lang, David G Munoz
Recent reports have characterized functional lymphatic vessels, which drain both fluid and immune cells from the CSF to the deep cervical lymph nodes, lining the dural sinuses in mice. If conserved in the human brain these vessels could have profound implications for neuroinflammatory and neurodegenerative diseases. We provide evidence of the presence of lymphatic vessels in human dura obtained at autopsy, at the level of the superior sagittal sinus, in 4 individuals. Immunohistochemistry for the lymphatic vessel endothelial cell marker podoplanin revealed the widespread presence of multiple structures with a distinct lumen distributed throughout the superior sagittal sinus...
November 10, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/29125824/intraluminal-valves-development-function-and-disease
#7
REVIEW
Xin Geng, Boksik Cha, Md Riaj Mahamud, R Sathish Srinivasan
The circulatory system consists of the heart, blood vessels and lymphatic vessels, which function in parallel to provide nutrients and remove waste from the body. Vascular function depends on valves, which regulate unidirectional fluid flow against gravitational and pressure gradients. Severe valve disorders can cause mortality and some are associated with severe morbidity. Although cardiac valve defects can be treated by valve replacement surgery, no treatment is currently available for valve disorders of the veins and lymphatics...
November 1, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/29125185/thymus-involvement-in-early-onset-myasthenia-gravis
#8
REVIEW
Mélanie A Cron, Solène Maillard, José Villegas, Frédérique Truffault, Muriel Sudres, Nadine Dragin, Sonia Berrih-Aknin, Rozen Le Panse
It has long been established that the thymus plays a central role in autoimmune myasthenia gravis (MG) because of either thymoma or thymic hyperplasia of lymphoproliferative origin. In this review, we discuss thymic changes associated with thymic hyperplasia and their implications in the development of an autoimmune response against the acetylcholine receptor (AChR).The hyperplastic MG thymus displays all the characteristics of tertiary lymphoid organs (TLOs): neoangiogenic processes with high endothelial venule and lymphatic vessel development, chemokine overexpression favoring peripheral cell recruitment, and ectopic germinal center development...
November 10, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/29122006/single-nucleotide-polymorphisms-in-the-angiogenic-and-lymphangiogenic-pathways-are-associated-with-lymphedema-caused-by-wuchereria-bancrofti
#9
Linda Batsa Debrah, Anna Albers, Alexander Yaw Debrah, Felix F Brockschmidt, Tim Becker, Christine Herold, Andrea Hofmann, Jubin Osei-Mensah, Yusif Mubarik, Holger Fröhlich, Achim Hoerauf, Kenneth Pfarr
BACKGROUND: Lymphedema (LE) is a chronic clinical manifestation of filarial nematode infections characterized by lymphatic dysfunction and subsequent accumulation of protein-rich fluid in the interstitial space-lymphatic filariasis. A number of studies have identified single nucleotide polymorphisms (SNPs) associated with primary and secondary LE. To assess SNPs associated with LE caused by lymphatic filariasis, a cross-sectional study of unrelated Ghanaian volunteers was designed to genotype SNPs in 285 LE patients as cases and 682 infected patients without pathology as controls...
November 9, 2017: Human Genomics
https://www.readbyqxmd.com/read/29117787/reprogramming-of-adult-peripheral-blood-cells-into-human-induced-pluripotent-stem-cells-as-a-safe-and-accessible-source-of-endothelial-cells
#10
Pavel Simara, Lenka Tesarova, Daniela Rehakova, Simon Farkas, Barbara Salingova, Katerina Kutalkova, Eva Vavreckova, Pavel Matula, Petr Matula, Lenka Veverkova, Irena Koutna
New approaches in regenerative medicine and vasculogenesis have generated a demand for sufficient numbers of human endothelial cells (ECs). ECs and their progenitors (EPCs) reside on the interior surface of blood and lymphatic vessels or circulate in peripheral blood; however, their numbers are limited and they are difficult to expand after isolation. Recent advances in human induced pluripotent stem cell (hiPSC) research have opened possible avenues to generate unlimited numbers of ECs from easily accessible cell sources, such as the peripheral blood...
November 8, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29098022/therapeutic-effects-of-protein-kinase-n3-small-interfering-rna-and-doxorubicin-combination-therapy-on-liver-and-lung-metastases
#11
Yoshiyuki Hattori, Takuto Kikuchi, Mari Nakamura, Kei-Ichi Ozaki, Hiraku Onishi
It has been reported that suppression of protein kinase N3 (PKN3) expression in vascular and lymphatic endothelial cells results in the inhibition of tumor progression and lymph node metastasis formation. The present study investigated whether combination therapy of small interfering RNA (siRNA) against PKN3 and doxorubicin (DXR) could increase therapeutic efficacy against liver and lung metastases. In vitro transfection of PKN3 siRNA into PKN3-positive MDA-MB-231, LLC, and Colon 26 cells and PKN3-negative MCF-7 cells did not inhibit cell growth and did not increase sensitivity to DXR...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29095088/vegf-a121a-binding-to-neuropilins-a-concept-revisited
#12
Sarvenaz Sarabipour, Feilim Mac Gabhann
All known splice isoforms of vascular endothelial growth factor A (VEGF-A) can bind to the receptor tyrosine kinases VEGFR-1 and VEGFR-2. We focus here on VEGF-A121a and VEGF-A165a, two of the most abundant VEGF-A splice isoforms in human tissue (1) , and their ability to bind the Neuropilin co-receptors NRP1 and NRP2. The Neuropilins are key vascular, immune, and nervous system receptors on endothelial cells, neuronal axons, and regulatory T cells respectively. They serve as co-receptors for the Plexins in Semaphorin binding on neuronal and vascular endothelial cells, and for the VEGFRs in VEGF binding on vascular and lymphatic endothelial cells, and thus regulate the initiation and coordination of cell signaling by Semaphorins and VEGFs...
November 2, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/29083411/vascular-endothelial-growth-factor-c-ameliorates-renal-interstitial-fibrosis-through-lymphangiogenesis-in-mouse-unilateral-ureteral-obstruction
#13
Shoko Hasegawa, Toshiaki Nakano, Kumiko Torisu, Akihiro Tsuchimoto, Masahiro Eriguchi, Naoki Haruyama, Kosuke Masutani, Kazuhiko Tsuruya, Takanari Kitazono
Renal fibrosis is the final common pathway of chronic kidney diseases. Lymphatic vessel (LV) proliferation is found in human renal diseases and other fibrotic diseases, suggesting that lymphangiogenesis is associated with the progression or suppression of kidney diseases. However, the purpose of LV proliferation is not completely understood. We investigated the effect of vascular endothelial growth factor (VEGF)-C on lymphangiogenesis, inflammation, and fibrosis in the mouse kidney using the unilateral ureteral obstruction (UUO) model...
October 30, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29075785/sparc-suppresses-lymph-node-metastasis-by-regulating-the-expression-of-vegfs-in-ovarian-carcinoma
#14
Fenghui Peng, Yanping Zhong, Yunfeng Liu, Yueming Zhang, Yihong Xie, Yingxin Lu, Xinyin Zhang, Danrong Li
Lymph node metastasis is one of the most valuable determinants for the prognosis of ovarian cancer. However, the molecular mechanisms underlying lymphangiogenesis in ovarian cancer is still poorly understood. Secreted protein acidic and rich in cysteine (SPARC), a Ca2+-binding matricellular glycoprotein that modulates cell adhesion, migration and differentiation, is thought to play a decisive role in tumor metastasis. Vascular endothelial growth factor (VEGF)-C and VEGF-D contributes to tumor-associated lymphatic vessel growth, enhancing the metastatic spread of tumor cells to lymph nodes...
December 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29066622/microrna-155-induction-via-tnf-%C3%AE-and-ifn-%C3%AE-suppresses-expression-of-programmed-death-ligand-1-pd-l1-in-human-primary-cells
#15
Daniel Yee, Kunal M Shah, Mark C Coles, Tyson V Sharp, Dimitris Lagos
Programmed death ligand-1 (PD-L1) is a critical regulator of T cell function contributing to peripheral immune tolerance. Although it has been shown that posttranscriptional regulatory mechanisms control PD-L1 expression in cancer, it remains unknown whether such regulatory loops operate also in non-transformed cells. Here we studied PD-L1 expression in human dermal lymphatic endothelial cells (HDLECs), which play key roles in immunity and cancer. Treatment of HDLECs with the pro-inflammatory cytokines IFN-γ and TNF-α synergistically upregulated PDL1 expression...
October 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29066220/de-adhesion-dynamics-of-melanoma-cells-from-brain-endothelial-layer
#16
Béla Varga, Réka Anita Domokos, Csilla Fazakas, Imola Wilhelm, István A Krizbai, Zsolt Szegletes, Csilla Gergely, György Váró, Attila G Végh
Metastasis formation is a complex and not entirely understood process. The poorest prognosis and the most feared complications are associated to brain metastases. Melanoma derived brain metastases show the highest prevalence. Due to the lack of classical lymphatic drainage, in the process of brain metastases formation the haematogenous route is of primordial importance. The first and crucial step in this multistep process is the establishment of firm adhesion between the blood travelling melanoma cells and the tightly connected layer of the endothelium, which is the fundamental structure of the blood-brain barrier...
October 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29065177/mir-27a-induced-by-colon-cancer-cells-in-hlecs-promotes-lymphangiogenesis-by-targeting-smad4
#17
Qi Xu, Jin-Lu Tong, Chen-Peng Zhang, Qian Xiao, Xiao-Lin Lin, Xiu-Ying Xiao
AIM: Metastasis of tumor cells occurs through lymphatic vessels, blood vessels and transcoelomic spreading. Growing evidence from in vivo and in vitro studies has indicated that tumor lymphangiogenesis facilitates metastasis. However, the regulation of lymphangiogenesis in colon cancer remains unclear. The aims of this study were to identify key miRNAs in colon cancer lymphangiogenesis and to investigate its target and mechanism. METHODS: miRNA microarray analysis was conducted to identify miRNAs in human lymphatic endothelial cells (HLECs) that were regulated by co-cultured human colon cancer cells...
2017: PloS One
https://www.readbyqxmd.com/read/29063612/endothelial-tubes-another-window-into-lymphatic-function
#18
Kim A Dora, Dirk F van Helden
The endothelium isolated as an intact tube of cells offers a powerful approach to study endothelial cell function. This article is protected by copyright. All rights reserved.
October 24, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/29057961/pannexin1-links-lymphatic-function-to-lipid-metabolism-and-atherosclerosis
#19
Filippo Molica, Merlijn J Meens, Juan Dubrot, Avigail Ehrlich, Christel L Roth, Sandrine Morel, Graziano Pelli, Laurent Vinet, Vincent Braunersreuther, Osman Ratib, Marc Chanson, Stephanie Hugues, Eliana Scemes, Brenda R Kwak
Extracellular ATP is a central signaling molecule in inflammatory responses. Pannexin1 (Panx1) channels release ATP in a controlled manner and have been implicated in various inflammatory pathologies, but their role in atherogenesis remains elusive. Using atherosclerosis-susceptible mouse models with ubiquitous deletion of Panx1 (Panx1 (-/-) Apoe (-/-) ) or with Cre recombinase-mediated deletion of Panx1 in endothelial cells and monocytes (Tie2-Cre (Tg) Panx1 (fl/fl) Apoe (-/-) ; Panx1 (del) Apoe (-/-) ), we identified a novel role for Panx1 in the lymphatic vasculature...
October 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29046873/embryonic-stem-cell-like-subpopulations-in-venous-malformation
#20
Elysia M S Tan, Sam Duro Siljee, Helen D Brasch, Susana Enriquez, Swee T Tan, Tinte Itinteang
BACKGROUND: Venous malformation (VM) consists of a network of ectatic anomalous thin-walled venous channels. A role for an activating TIE2 mutation in the development of the dilated luminal vessels in VM, and its proposed involvement of embryonic stem cells (ESCs), led us to investigate the expression of ESC markers in subcutaneous VM (SCVM) and intramuscular VM (IMVM). METHODS: Formalin-fixed paraffin-embedded sections of SCVM from seven patients and IMVM samples from seven patients were analyzed for the expression of Nanog, pSTAT3, OCT4, SOX2, SALL4, and CD44, using 3,3'-diaminobenzidine (DAB) immunohistochemical (IHC) staining...
2017: Frontiers in Medicine
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