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https://www.readbyqxmd.com/read/29589176/photodynamic-inactivation-in-the-expression-of-the-candida-albicans-genes-als3-hwp1-bcr1-tec1-cph1-and-efg1-in-biofilms
#1
Fernanda Freire, Patrícia Pimentel de Barros, Cristiane Aparecida Pereira, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge
The objective of this study was to evaluate the effects of photodynamic inactivation (PDI) on Candida albicans biofilms, evaluating its effects on gene expression of ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 by yeast. Three samples of C. albicans were used in this study: a clinical sample from a patient with HIV (39S), a clinical sample from a patient with denture stomatitis lesion (Ca30), and a standard strain ATCC 18804. The quantification of gene expression was related to the production of those genes in the samples referred above using quantitative polymerase chain reaction (qPCR) assay in real time...
March 27, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29470441/effect-of-silver-emitting-filler-on-antimicrobial-and-mechanical-properties-of-soft-denture-lining-material
#2
Ewa Jabłońska-Stencel, Wojciech Pakieła, Anna Mertas, Elżbieta Bobela, Jacek Kasperski, Grzegorz Chladek
Colonization of silicone-based soft lining materials by pathogenic yeast-type fungi is a common problem associated with the use of dentures. In this study, silver sodium hydrogen zirconium phosphate (SSHZP) was introduced into polydimethylsiloxane-based material as an antimicrobial filler at concentrations of 0.25, 0.5, 1, 2, 4, 6, 8, 10, 12, and 14% ( w / w ). The in vitro antimicrobial efficacy was investigated. Candida albicans was used as a characteristic representative of pathogenic oral microflora. Staphylococcus aureus and Escherichia coli were used as the typical Gram-positive and Gram-negative bacterial strains, respectively...
February 22, 2018: Materials
https://www.readbyqxmd.com/read/29460527/management-of-denture-stomatitis-a-narrative-review
#3
D Di Stasio, D Lauritano, G Minervini, R S Paparella, M Petruzzi, A Romano, V Candotto, A Lucchese
Candida albicans is a typical commensal microorganism of the oral cavity that can become virulent in certain conditions and cause denture stomatitis (DS). Many conditions can predispose for DS, including local and systemic alterations. The therapeutical approach to DS is various and both topical and systemic alternatives can be considered. Azoles molecules are the most common used, both topically and systemically, but in recent years various alternatives have been proposed. This review aims to examine the scientific literature to evaluate the effectiveness of conventional therapies and the potential of the new ones...
January 2018: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29458673/denture-associated-biofilm-infection-in-three-dimensional-oral-mucosal-tissue-models
#4
Daniel J Morse, Melanie J Wilson, Xiaoqing Wei, Michael A O Lewis, David J Bradshaw, Craig Murdoch, David W Williams
PURPOSE: In vitro analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The Candida-related infection, denture-associated oral candidosis, affects up to 60 % of denture wearers and manifests as inflammation of palatal tissues contacting the denture-fitting surface. Commercially available three-dimensional tissue models can be used to study infection, but their use is limited for many academic research institutions, primarily because of the substantial purchase costs...
January 11, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29428169/effectiveness-of-a-combination-denture-cleaning-method-versus-a-mechanical-method-comparison-of-denture-cleanliness-patient-satisfaction-and-oral-health-related-quality-of-life
#5
Yuya Baba, Yusuke Sato, Gaku Owada, Shunsuke Minakuchi
PURPOSE: Effective denture plaque control is necessary in elderly individuals to prevent oral and systemic diseases. However, comparative studies of denture cleaning methods are limited, especially those investigating patient satisfaction. The present study aimed to evaluate effectiveness of a mechanical denture cleaning method versus a combination of mechanical and chemical methods in terms of denture cleanliness, patient satisfaction, and oral health-related quality of life (OHRQoL)...
February 7, 2018: Journal of Prosthodontic Research
https://www.readbyqxmd.com/read/29423993/cytokine-gene-polymorphism-in-denture-stomatitis-patients-a-clinical-study
#6
Aysun Coşkun Keşoğlu, Canan Bural, Gonca Erköse Genç, Zayre Erturan, Çiğdem Çınar, Fatma Oğuz, Tayfun Bilgin, Hakan Bilhan
OBJECTIVE: This clinical study investigated the association between cytokine gene polymorphism and Candida growth in DS patients. SUBJECTS AND METHODS: Saliva and blood samples of 160 complete denture wearers (80 healthy controls and 80 with DS) were collected for mycological and gene polymorphism testing, respectively. Salivary Candida growth and TNF-α, TGF-β, IL-6 and IL-10 genotypes were investigated. Data were analyzed using Student t-test, Mann-Whitney U, Chi-square analysis and Continuity (yates) correction tests (p<...
February 9, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29411720/evaluation-of-candida-albicans-biofilm-formation-on-various-parts-of-implant-material-surfaces
#7
C Gökmenoglu, N B Kara, M Beldüz, A Kamburoğlu, I Tosun, E Sadik, C Kara
AIMS: Candida albicans adhesion to any oral substrata is the first and essential stage in forming a pathogenic fungal biofilm. In general, yeast cells have remarkable potential to adhere to host surfaces, such as teeth or mucosa, and to artificial, non-biological surfaces, such as dental materials. C. albicans adhesion to denture materials is widely recognized as the main reason for the development of stomatitis. This study compared the susceptibility of different parts of the implant system with C...
January 2018: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29402621/evaluation-of-anti-enzyme-properties-of-origanum-vulgare-essential-oil-against-oral-candida-albicans
#8
L Pradebon Brondani, T Alves da Silva Neto, R Antonio Freitag, R Guerra Lund
This study aimed to evaluate the anti-enzymatic activity of Origanum vulgare (oregano) essential oil against 15 strains of Candida albicans. Candida albicans samples were isolated from the oral mucosa of patients with denture stomatitis treated in a Dentistry school on a public university. Preparation of the inoculum was performed with a suspension of C. albicans reactivated 24h earlier in 5mL of sterile phosphate buffer saline (PBS) adjusted to a 0.5-turbidity on the MacFarland scale (1,5×108 UFC/mL). The essential oil was obtained by hydrodistillation in a Clevenger-type machine and analyzed by gas chromatography...
February 2, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29402540/nano-graphene-oxide-incorporated-into-pmma-resin-to-prevent-microbial-adhesion
#9
Jung-Hwan Lee, Jeong-Ki Jo, Dong-Ae Kim, Kapil Dev Patel, Hae-Won Kim, Hae-Hyoung Lee
OBJECTIVE: Although polymethyl methacrylate (PMMA) is widely used as a dental material, a major challenge of using this substance is its poor antimicrobial (anti-adhesion) effects, which increase oral infections. Here, graphene-oxide nanosheets (nGO) were incorporated into PMMA to introduce sustained antimicrobial-adhesive effects by increasing the hydrophilicity of PMMA. METHODS: After characterizing nGO and nGO-incorporated PMMA (up to 2wt%) in terms of morphology and surface characteristics, 3-point flexural strength and hardness were evaluated...
February 2, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29364341/determination-of-the-effective-anticandidal-concentration-of-denture-cleanser-tablets-on-some-denture-base-resins
#10
Yeliz Hayran, Işıl Sarikaya, Ali Aydin, Yadel Hazır Tekin
Although the effectiveness of chemical cleansing against Candida albicans biofilm has been shown, the effective concentration of denture cleanser tablets has not been studied. The aim of this study was to assess the effect of three denture materials against Candida albicans biofilm and to determine effective concentrations of denture cleanser tablets. The surface-roughness of Acron-hi™, QC-20™ and Deflex™ (n=45 per resin) resins was standardized by using a profilometer and their contact angle or surface free energy was calculated...
January 18, 2018: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/29291436/superhydrophilic-co-polymer-coatings-on-denture-surfaces-reduce-candida-albicans-adhesion-an-in-vitro-study
#11
Masahiro Hirasawa, Chiaki Tsutsumi-Arai, Kensuke Takakusaki, Toyohisa Oya, Kenji Fueki, Noriyuki Wakabayashi
OBJECTIVE: In this study, we aimed to investigate denture-base-resin coatings prepared with a crosslinkable co-polymer containing sulfobetaine methacrylamide (SBMAm) and the relationship between their surface characteristics and the initial adhesion of Candida albicans (C. albicans). METHODS: Acrylic resin discs were coated with co-polymers containing various concentrations of SBMAm and N,N'-(4,7,10-trioxa-1,13-tridecadiamine) diacrylamide (JDA) as crosslinking agent...
March 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29286435/visualization-of-biofilm-formation-in-candida-albicans-using-an-automated-microfluidic-device
#12
Megha Gulati, Craig L Ennis, Diana L Rodriguez, Clarissa J Nobile
Candida albicans is the most common fungal pathogen of humans, causing about 15% of hospital-acquired sepsis cases. A major virulence attribute of C. albicans is its ability to form biofilms, structured communities of cells attached to biotic and abiotic surfaces. C. albicans biofilms can form on host tissues, such as mucosal layers, and on medical devices, such as catheters, pacemakers, dentures, and joint prostheses. Biofilms pose significant clinical challenges because they are highly resistant to physical and chemical perturbations, and can act as reservoirs to seed disseminated infections...
December 14, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29279769/biofilm-formation-on-denture-base-resin-including-zno-cao-and-tio-2-nanoparticles
#13
Melissa Anwander, Martin Rosentritt, Sibylle Schneider-Feyrer, Sebastian Hahnel
PURPOSE: This laboratory study aimed to investigate the effect of doping an acrylic denture base resin material with nanoparticles of ZnO, CaO, and TiO2 on biofilm formation. MATERIALS AND METHODS: Standardized specimens of a commercially available cold-curing acrylic denture base resin material were doped with 0.1, 0.2, 0.4, or 0.8 wt% commercially available ZnO, CaO, and TiO2 nanopowder. Energy dispersive X-ray spectroscopy (EDX) was used to identify the availability of the nanoparticles on the surface of the modified specimens...
December 2017: Journal of Advanced Prosthodontics
https://www.readbyqxmd.com/read/29250982/antimicrobial-activity-of-denture-adhesive-associated-with-equisetum-giganteum-and-punica-granatum-enriched-fractions-against-candida-albicans-biofilms-on-acrylic-resin-surfaces
#14
Nara Ligia Martins Almeida, Luiz Leonardo Saldanha, Rafaela Alves da Silva, Karen Henriette Pinke, Eliane Ferraz da Costa, Vinicius Carvalho Porto, Anne Lígia Dokkedal, Vanessa Soares Lara
Candida biofilms adhere to the internal surface of removable dentures, which is an etiological factor in the pathogenesis of denture stomatitis (DS). Adhesive materials are used at the base of maxillary complete dentures to improve their retention and chewing qualities. This article reports the antimicrobial activity of the enriched fractions of Equisetum giganteum and Punica granatum incorporated into a denture adhesive against C. albicans biofilm. The biofilms were induced on the surface of heat-cured acrylic resin specimens that were previously treated with a mixture of adhesive/herb extracts...
January 2018: Biofouling
https://www.readbyqxmd.com/read/29246167/the-candida-species-that-are-important-for-the-development-of-atrophic-glossitis-in-xerostomia-patients
#15
Sachika Nakamura, Mariko R Okamoto, Ken Yamamoto, Akihisa Tsurumoto, Yoko Yoshino, Hiroshi Iwabuchi, Ichiro Saito, Nobuko Maeda, Yoichi Nakagawa
BACKGROUND: The purpose of this study was to clarify the species of Candida that are important for the development of atrophic glossitis in xerostomia patients. METHODS: A total of 231 patients with subjective dry mouth were enrolled in the present study. Logistic regression analysis was performed to clarify the contribution of each Candida species and other variables to the development of atrophic glossitis. The dependent variable was the absence/presence of atrophic glossitis...
December 16, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/29228383/oral-fungal-bacterial-biofilm-models-in-vitro-a-review
#16
Marlene Chevalier, Stephane Ranque, Isabelle Prêcheur
Inclusion of fungi as commensals in oral biofilm is an important innovation in oral biology, and this work aimed to review the literature on the available biofilm and related disease in vitro models. Actually, thousands of bacterial and around one hundred of fungal phylotypes can colonize the oral cavity. Taxonomic profiling combined with functional expression analysis has revealed that Candida albicans, Streptococcus mutans and prominent periodontopathogens are not always present or numerically important in candidiasis, caries, or periodontitis lesions...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29198763/antimicrobial-photodynamic-therapy-mediated-by-photodithazine-%C3%A2-in-the-treatment-of-denture-stomatitis-a-case-report
#17
Fernanda Alves, Gabriela Caroline Alonso, Juliana Cabrini Carmello, Ewerton Garcia de Oliveira Mima, Vanderlei Salvador Bagnato, Ana Cláudia Pavarina
Antimicrobial Photodynamic Therapy (aPDT) mediated by Photodithazine® (PDZ) has shown efficacy in the inactivation of Candida spp. in in vitro and in vivo studies. This preliminary study reports five clinical cases of patients with denture stomatitis (DS) treated with PDZ-mediated aPDT. Five individuals diagnosed with DS were selected and submitted to aPDT 3 times a week for 15days (6 sessions). In each session, 200mg/L of PDZ gel was applied on the upper prostheses and on the palate of the patients for 20minutes, then, illuminated by a light emitting diode at 660nm (50J/cm2 )...
November 30, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29178772/antimicrobial-action-and-long-term-effect-of-overnight-denture-cleansers
#18
Amanda Peracini, Ingrid Machado Andrade, Viviane Cássia Oliveira, Ana Paula Macedo, Claudia Helena Silva-Lovato, Valéria Oliveira Pagnano, Evandro Watanabe, Helena de Freitas Oliveira Paranhos
PURPOSE: To evaluate two denture cleansers for overnight soaking (0.5% sodium hypochlorite and peroxide alkaline) regarding efficacy against Candida spp. biofilms (randomized clinical trial) and their effects on the physical properties of a denture base acrylic resin simulating a period of 5 years of use (laboratory study). The Candida spp. were identified and their resistance to main antifungal agents was evaluated. METHODS: 32 complete denture wearers were instructed to brush their dentures three times a day and to soak them (≥8 hours) in: control - water (C); alkaline peroxide (AP); or 0...
April 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29142649/adherence-of-candida-to-complete-denture-surfaces-in-vitro-a-comparison-of-conventional-and-cad-cam-complete-dentures
#19
Afnan F Al-Fouzan, Lamya A Al-Mejrad, Ahmed M Albarrag
PURPOSE: The goal of this study was to compare the adhesion of Candida albicans to the surfaces of CAD/CAM and conventionally fabricated complete denture bases. MATERIALS AND METHODS: Twenty discs of acrylic resin poly (methyl methacrylate) were fabricated with CAD/CAM and conventional procedures (heat-polymerized acrylic resin). The specimens were divided into two groups: 10 discs were fabricated using the CAD/CAM procedure (Wieland Digital Denture Ivoclar Vivadent), and 10 discs were fabricated using a conventional flasking and pressure-pack technique...
October 2017: Journal of Advanced Prosthodontics
https://www.readbyqxmd.com/read/29142640/evaluation-of-c-albicans-and-s-mutans-adherence-on-different-provisional-crown-materials
#20
Gulsum Sayin Ozel, Mehmet Burak Guneser, Ozgur Inan, Ayce Unverdi Eldeniz
PURPOSE: Bacterial adhesion on provisional crown materials retained for a long time can influence the duration for which permanent prosthetic restorations can be healthily worn in the oral cavity. The aim of this study was to compare seven different commonly used provisional crown materials with regard to Streptococcus mutans and Candida albicans surface adhesion. MATERIALS AND METHODS: For each group, twenty specimens of the provisional fixed prosthodontic materials TemDent (Schütz), Imıdent (Imıcryl), Tab 2000 (Kerr), Structur Premium (Voco), Systemp (Ivoclar Vivadent), Acrytemp (Zhermack), and Takilon-BBF (Takilon) were prepared (diameter, 10...
October 2017: Journal of Advanced Prosthodontics
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