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Candida denture

Nathalia Albrecht, Tatiana Kelly DA Silva Fidalgo, Maria José Santos DE Alencar, Lucianne Cople Maia, Vanessa Migliorini Urban, Karin Hermana Neppelenbroek, Kátia Rodrigues Reis
Two soft denture lining materials (SC-Soft Confort and TS-Trusoft) were investigated with and without the addition of 1.0% of chlorhexidine diacetate (1.0% CHX). To assess peel bond strength, specimens (75×10×6 mm) were submitted to a peel test at 10 mm/min immediately and after 24 h. To evaluate Candida albicans growth inhibition, disc of specimens (10×3 mm) were immersed in a solution with 3×106 CFU/mL of C. albicans, and spectral measurements were made following immersion in MTT solution for 2, 4, and 6 days...
June 20, 2018: Dental Materials Journal
S An, R B Judge, R H Wong, M H Arzmi, J E Palamara, S G Dashper
BACKGROUND: This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity. METHODS: Fifty-four heat cured rectangular DBR specimens (64 x 10 x 3.3 ± 0.2 mm) containing nine concentrations of PTMC between 0 - 5% (wt/wt) were fabricated and subjected to a three-point bending test. A phytoncide release bioassay was developed using DBR containing 0% and 2...
June 20, 2018: Australian Dental Journal
Seenivasan Madhan Kumar, V Anand Kumar, Parathasarthy Natarajan, Gayathri Sreenivasan
Objectives: To evaluate the in vitro growth inhibition of Candida albicans , in the soft-liner material and Shore A hardness from resin-based denture soft lining materials modified by neem or garlic incorporation. Materials and Methods: Resin discs were prepared with poly methyl methacrylate (PMMA) and soft liners incorporated with varying concentrations of neem or garlic. For antifungal activity, resin discs were placed on agar plates inoculated with C. albicans and were evaluated after 2, 4, and 7 days using the streaking method...
May 2018: Journal of International Society of Preventive & Community Dentistry
Saeid Mahdavi Omran, Maryam Rezaei Dastjerdi, Maryam Zuashkiani, Vahid Moqarabzadeh, Mojtaba Taghizadeh-Armaki
Background: Candida -associated denture stomatitis (CADS) is a common fungal infection in people who wear dentures. The main objective of this study was to make molecular identification of causative agents of CADS and in vitro antifungal susceptibility testing (AFST) in the Iranian patients with denture stomatitis. Methods: A total of 134 Candida spp. were obtained from patients with denture stomatitis. The Candida spp. were identified using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) involving the universal internal transcribed spacer (ITS1 and ITS4) primers, which were subjected to digestion with MspI and BlnI restriction enzymes...
2018: BioMed Research International
Muneaki Tamura, Marni E Cueno, Kazumasa Abe, Noriaki Kamio, Kuniyasu Ochiai, Kenichi Imai
Candida albicans causes opportunistic fungal infections usually hidden among more dominant bacteria and does not exhibit high pathogenicity in vivo. Among the elderly, due to reduced host resistance to pathogens attributable to immunoscenesence, oral candidiasis is more likely to develop often leading to systemic candidiasis. Surface pre-reacted glass ionomer filler (S-PRG filler) is an ion-releasing functional bioactive glass that can release and recharge six ions which in turn strengthens tooth structure, inhibits demineralization arising from dental caries, and suppresses dental plaque accumulation...
June 6, 2018: Cell Stress & Chaperones
Nelson Ndegwa, Alexander Ploner, Zhiwei Liu, Ann Roosaar, Tony Axéll, Weimin Ye
Poor oral health may be involved in the pathogenesis of gastric cancer, however some aspects have not been explored. Further, for previously studied aspects, e.g. tooth-loss, the findings are inconsistent. We conducted a prospective cohort study of 19,831 participants from Uppsala, Sweden, cancer-free at baseline in 1973-74 and followed until 2012 through linkage to national registers. We found that individuals with fewest teeth at baseline had an increased risk of gastric cancer relative to subjects with all examined teeth present (p=1...
June 6, 2018: International Journal of Cancer. Journal International du Cancer
A L F Procópio, Rafaela Alves da Silva, Janaina Gomes Maciel, Carolina Yoshi Campos Sugio, S Soares, V M Urban, K H Neppelenbroek
The coadjutant method for denture cleansing most used by denture wearers is immersion in chemical agents, which are toxic when in direct contact with cells. However, clinically, the contact between these chemical agents and prosthetic tissues does not occur directly, but rather with what remained impregnated into acrylic bases, even after rinsing the disinfected dentures. This study evaluated the antimicrobial and cytotoxic effects of a denture acrylic resin after successive cycles of daily overnight immersion in 1% sodium hypochlorite (1%NaClO) and 2% chlorhexidine digluconate (2%CHX), simulating the periods of 9 months or 1...
May 25, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Mirjana Perić, Rade Živković, Aleksandra Milić Lemić, Milena Radunović, Biljana Miličić, Valentina Arsić Arsenijević
OBJECTIVES: 1) To select patients with Candida-related denture stomatitis (DS) and to study possible risk factors associated with DS, 2) to evaluate the severity of DS according to Newton's classification, and 3) to investigate the association between the presence of non-albicans Candida spp. (NAC) or mixed Candida spp. and the severity of DS. STUDY DESIGN: Eighty-two patients with Candida-positive DS have undergone 1) filling in the interview questionnaire, 2) clinical examination, and 3) microbiologic examination...
March 22, 2018: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Yusaku Koseki, Rika Tanaka, Hiroshi Murata
We evaluated effectiveness of tea tree oil (TO) and lemongrass oil (LO) for removal of Candida biofilm from denture base resin and their influence on that surface. Biofilm of C. albicans was formed on resins, and immersed in various concentrations of each oil and distilled water (DW). The biofilm removal effect was determined by incubating specimens in RPMI medium containing Alamar blue (AB) and measuring absorbance. Wear test was also conducted, and surface condition of resins was determined using laser scanning microscope and digital microscope...
May 2, 2018: Dental Materials Journal
Indu Raj, Miran Mozetic, Jayachandran V Prabhu, Jiya Jose, Sabu Thomas, Nandakumar Kalarikkal
Antimicrobial, antibiofilm adherent, fracture resistant nano Zinc Oxide (ZnO NP) formulations based on poly methyl methacrylate (PMMA) matrix were developed by ex situ compression moulding technique in a facile manner. These formulations demonstrated potent, long-term biofilm-resisting effects against Candida Albicans (9000 CFU to 1000CFU) and Streptococcal Mutans. Proposed mechanism of biofilm resistance was the release of metallic ions/metal oxide by 'particle-corrosion'. MTT and cellular proliferation assays confirmed both qualitatively and quantitatively equal human skin fibroblast cell line proliferations (approximately 75%) on both PMMA/ZnO formulation and on neat PMMA...
May 4, 2018: Nanotechnology
André M de Senna, Martha M F Vieira, Rosa Maria Machado-de-Sena, Aparecido O Bertolin, Silvia C Núñez, Martha S Ribeiro
Denture stomatitis (DS) is the most common oral fungal infection in denture wearers. Photodynamic inactivation (PDI) has been showing to be an effective technique in vivo against fungi, including fungal infections in the oral cavity. The disinfection of both oral mucosa and denture may represent a real advantage in terms of fungus control. This clinical study was designed to explore methylene blue (MB)-mediated PDI on oral mucosa and prosthesis of patients with DS. Subjects with DS were divided into two groups...
June 2018: Photodiagnosis and Photodynamic Therapy
Fernanda Freire, Patrícia Pimentel de Barros, Cristiane Aparecida Pereira, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge
The objective of this study was to evaluate the effects of photodynamic inactivation (PDI) on Candida albicans biofilms, evaluating its effects on gene expression of ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 by yeast. Three samples of C. albicans were used in this study: a clinical sample from a patient with HIV (39S), a clinical sample from a patient with denture stomatitis lesion (Ca30), and a standard strain ATCC 18804. The quantification of gene expression was related to the production of those genes in the samples referred above using quantitative polymerase chain reaction (qPCR) assay in real time...
March 27, 2018: Lasers in Medical Science
Ewa Jabłońska-Stencel, Wojciech Pakieła, Anna Mertas, Elżbieta Bobela, Jacek Kasperski, Grzegorz Chladek
Colonization of silicone-based soft lining materials by pathogenic yeast-type fungi is a common problem associated with the use of dentures. In this study, silver sodium hydrogen zirconium phosphate (SSHZP) was introduced into polydimethylsiloxane-based material as an antimicrobial filler at concentrations of 0.25, 0.5, 1, 2, 4, 6, 8, 10, 12, and 14% ( w / w ). The in vitro antimicrobial efficacy was investigated. Candida albicans was used as a characteristic representative of pathogenic oral microflora. Staphylococcus aureus and Escherichia coli were used as the typical Gram-positive and Gram-negative bacterial strains, respectively...
February 22, 2018: Materials
D Di Stasio, D Lauritano, G Minervini, R S Paparella, M Petruzzi, A Romano, V Candotto, A Lucchese
Candida albicans is a typical commensal microorganism of the oral cavity that can become virulent in certain conditions and cause denture stomatitis (DS). Many conditions can predispose for DS, including local and systemic alterations. The therapeutical approach to DS is various and both topical and systemic alternatives can be considered. Azoles molecules are the most common used, both topically and systemically, but in recent years various alternatives have been proposed. This review aims to examine the scientific literature to evaluate the effectiveness of conventional therapies and the potential of the new ones...
January 2018: Journal of Biological Regulators and Homeostatic Agents
Daniel J Morse, Melanie J Wilson, Xiaoqing Wei, Michael A O Lewis, David J Bradshaw, Craig Murdoch, David W Williams
PURPOSE: In vitro analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The Candida-related infection, denture-associated oral candidosis, affects up to 60 % of denture wearers and manifests as inflammation of palatal tissues contacting the denture-fitting surface. Commercially available three-dimensional tissue models can be used to study infection, but their use is limited for many academic research institutions, primarily because of the substantial purchase costs...
March 2018: Journal of Medical Microbiology
Yuya Baba, Yusuke Sato, Gaku Owada, Shunsuke Minakuchi
PURPOSE: Effective denture plaque control is necessary in elderly individuals to prevent oral and systemic diseases. However, comparative studies of denture cleaning methods are limited, especially those investigating patient satisfaction. The present study aimed to evaluate effectiveness of a mechanical denture cleaning method versus a combination of mechanical and chemical methods in terms of denture cleanliness, patient satisfaction, and oral health-related quality of life (OHRQoL)...
February 7, 2018: Journal of Prosthodontic Research
Aysun Coşkun Keşoğlu, Canan Bural, Gonca Erköse Genç, Zayre Erturan, Çiğdem Çınar, Fatma Oğuz, Tayfun Bilgin, Hakan Bilhan
OBJECTIVE: This clinical study investigated the association between cytokine gene polymorphism and Candida growth in DS patients. SUBJECTS AND METHODS: Saliva and blood samples of 160 complete denture wearers (80 healthy controls and 80 with DS) were collected for mycological and gene polymorphism testing, respectively. Salivary Candida growth and TNF-α, TGF-β, IL-6 and IL-10 genotypes were investigated. Data were analyzed using Student t-test, Mann-Whitney U, Chi-square analysis and Continuity (yates) correction tests (p<...
February 9, 2018: Oral Diseases
C Gökmenoglu, N B Kara, M Beldüz, A Kamburoğlu, I Tosun, E Sadik, C Kara
AIMS: Candida albicans adhesion to any oral substrata is the first and essential stage in forming a pathogenic fungal biofilm. In general, yeast cells have remarkable potential to adhere to host surfaces, such as teeth or mucosa, and to artificial, non-biological surfaces, such as dental materials. C. albicans adhesion to denture materials is widely recognized as the main reason for the development of stomatitis. This study compared the susceptibility of different parts of the implant system with C...
January 2018: Nigerian Journal of Clinical Practice
L Pradebon Brondani, T Alves da Silva Neto, R Antonio Freitag, R Guerra Lund
This study aimed to evaluate the anti-enzymatic activity of Origanum vulgare (oregano) essential oil against 15 strains of Candida albicans. Candida albicans samples were isolated from the oral mucosa of patients with denture stomatitis treated in a Dentistry school on a public university. Preparation of the inoculum was performed with a suspension of C. albicans reactivated 24h earlier in 5mL of sterile phosphate buffer saline (PBS) adjusted to a 0.5-turbidity on the MacFarland scale (1,5×108 UFC/mL). The essential oil was obtained by hydrodistillation in a Clevenger-type machine and analyzed by gas chromatography...
March 2018: Journal de Mycologie Médicale
Jung-Hwan Lee, Jeong-Ki Jo, Dong-Ae Kim, Kapil Dev Patel, Hae-Won Kim, Hae-Hyoung Lee
OBJECTIVE: Although polymethyl methacrylate (PMMA) is widely used as a dental material, a major challenge of using this substance is its poor antimicrobial (anti-adhesion) effects, which increase oral infections. Here, graphene-oxide nanosheets (nGO) were incorporated into PMMA to introduce sustained antimicrobial-adhesive effects by increasing the hydrophilicity of PMMA. METHODS: After characterizing nGO and nGO-incorporated PMMA (up to 2wt%) in terms of morphology and surface characteristics, 3-point flexural strength and hardness were evaluated...
April 2018: Dental Materials: Official Publication of the Academy of Dental Materials
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