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Lignocellulosic enzymatic hydrolysis

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https://www.readbyqxmd.com/read/28208065/lignocellulosic-butanol-production-from-napier-grass-using-semi-simultaneous-saccharification-fermentation
#1
Chi-Ruei He, Yu-Yuan Kuo, Si-Yu Li
Napier grass is a potential feedstock for biofuel production because of its strong adaptability and wide availability. Compositional analysis has been done on Napier grass which was collected from a local area of Taiwan. By comparing acid- and alkali-pretreatment, it was found that the alkali-pretreatment process is favorable for Napier grass. An overall glucose yield of 0.82g/g-glucosetotal can be obtained with the combination of alkali-pretreatment (2.5wt% NaOH, 8wt% sample loading, 121°C, and a reaction time of 40min) and enzymatic hydrolysis (40FPU/g-substrate)...
January 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28188936/cultivation-of-podospora-anserina-on-soybean-hulls-results-in-an-efficient-enzyme-cocktail-for-plant-biomass-hydrolysis
#2
Miia R Mäkelä, Ourdia Bouzid, Diogo Robl, Harm Post, Mao Peng, Albert Heck, Maarten Altelaar, Ronald P de Vries
The coprophilic ascomycete fungus Podospora anserina was cultivated on three different plant biomasses, i.e. cotton seed hulls (CSH), soybean hulls (SBH) and acid-pretreated wheat straw (WS) for four days, and the potential of the produced enzyme mixtures was compared in the enzymatic saccharification of the corresponding lignocellulose feedstocks. The enzyme cocktail P. anserina produced after three days of growth on SBH showed superior capacity to release reducing sugars from all tested plant biomass feedstocks compared to the enzyme mixtures from CSH and WS cultures...
February 7, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28181082/growth-and-expression-of-relevant-metabolic-genes-of-clostridium-thermocellum-cultured-on-lignocellulosic-residues
#3
Vanessa O Leitão, Eliane F Noronha, Brenda R Camargo, Pedro R V Hamann, Andrei S Steindorff, Betania F Quirino, Marcelo Valle de Sousa, Cirano J Ulhoa, Carlos R Felix
The plant cell wall is a source of fermentable sugars in second-generation bioethanol production. However, cellulosic biomass hydrolysis remains an obstacle to bioethanol production in an efficient and low-cost process. Clostridium thermocellum has been studied as a model organism able to produce enzymatic blends that efficiently degrade lignocellulosic biomass, and also as a fermentative microorganism in a consolidated process for the conversion of lignocellulose to bioethanol. In this study, a C. thermocellum strain (designated B8) isolated from goat rumen was characterized for its ability to grow on sugarcane straw and cotton waste, and to produce cellulosomes...
February 8, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28176202/bioethanol-production-from-steam-exploded-rice-husk-by-recombinant-escherichia-coli-ko11
#4
Takamitsu Tabata, Yusuke Yoshiba, Tomonori Takashina, Kazuo Hieda, Norio Shimizu
Rice husk is one of the most abundant types of lignocellulosic biomass. Because of its significant amount of sugars, such as cellulose and hemicellulose, it can be used for the production of biofuels such as bioethanol. However, the complex structure of lignocellulosic biomass, consisting of cellulose, hemicellulose and lignin, is resistant to degradation, which limits biomass utilization for ethanol production. The protection of cellulose by lignin contributes to the recalcitrance of lignocelluloses to hydrolysis...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28169125/intensification-of-delignification-and-subsequent-hydrolysis-for-the-fermentable-sugar-production-from-lignocellulosic-biomass-using-ultrasonic-irradiation
#5
Preeti B Subhedar, Pearl Ray, Parag R Gogate
The present work deals with intensification of delignification and subsequent enzymatic hydrolysis of sustainable biomass such as groundnut shells, coconut coir and pistachio shells using ultrasound assisted approach so as to develop an economical approach for obtaining bioethanol. Process intensification, in the current context, is referred to as any improvements giving enhanced rates possibly with lower energy and chemical as well as enzyme requirement for delignification and hydrolysis respectively. Conventional processing for both delignification and enzymatic hydrolysis has also been investigated for establishing the degree of intensification...
January 21, 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28110130/enhanced-enzymatic-hydrolysis-of-sugarcane-bagasse-with-ferric-chloride-pretreatment-and-surfactant
#6
Hongdan Zhang, Guangying Ye, Yutuo Wei, Xin Li, Aiping Zhang, Jun Xie
A FeCl3 pretreatment methodology was developed to convert raw sugarcane bagasse to highly digestible pretreated solid and selectively extract up to ∼100% of the hemicellulose from lignocellulosic biomass. FeCl3 pretreated solids yielded a quite high fermentable sugar yield compared to the native material. In addition, characterization of raw material and pretreated solid by X-ray diffraction (XRD), scanning electron microscopy (SEM), and thermogravimetric (TG) analysis was carried out to better understand how hemicellulose removal affected subsequent enzymatic hydrolysis...
April 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28110129/identifying-and-overcoming-the-effect-of-mass-transfer-limitation-on-decreased-yield-in-enzymatic-hydrolysis-of-lignocellulose-at-high-solid-concentrations
#7
Jian Du, Yuan Cao, Guodong Liu, Jian Zhao, Xuezhi Li, Yinbo Qu
Cellulose conversion decreases significantly with increasing solid concentrations during enzymatic hydrolysis of insoluble lignocellulosic materials. Here, mass transfer limitation was identified as a significant determining factor of this decrease by studying the hydrolysis of delignified corncob residue in shake flask, the most used reaction vessel in bench scale. Two mass transfer efficiency-related factors, mixing speed and flask filling, were shown to correlate closely with cellulose conversion at solid loadings higher than 15% DM...
April 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28097168/purification-and-biochemical-properties-of-a-thermostable-haloalkaline-cellulase-from-bacillus-licheniformis-amf-07-and-its-application-for-hydrolysis-of-different-cellulosic-substrates-to-bioethanol-production
#8
Fatemeh Azadian, Arastoo Badoei-Dalfard, Abdolhamid Namaki-Shoushtari, Mehdi Hassanshahian
A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on SDS- PAGE with a molecular weight of 37 kDa. The CMCase enzyme was highly active and stable over broad ranges of temperature (40-80ºC), pH (6...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28063358/chemical-input-reduction-in-the-arabinoxylan-and-lignocellulose-alkaline-extraction-and-xylooligosaccharides-production
#9
Franciane Cristina de Figueiredo, Ana Flavia Azevedo Carvalho, Michel Brienzo, Tania Sila Campioni, Pedro de Oliva-Neto
Lignocellulosic material breakdown by hydrolysis is an important step to open new perspectives for bioenergy and special foods production like prebiotic xylooligosaccharides. Improvement of lignocellulose and arabinoxylan alkaline extraction from sugarcane bagasse and enzymatic hydrolysis were performed. Treatments 1 (10% KOH at 70°C), 3 (5% KOH at 121°C) and ZD method (24% KOH at 35°C) showed solid lignocellulose recovery of respectively 75.2%, 74.2% and 73%. A range of 24.8-27% extracted material with high arabinoxylan content (72...
March 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28062914/determination-of-kinetics-and-heat-of-hydrolysis-for-non-homogenous-substrate-by-isothermal-calorimetry
#10
D Tafoukt, A Soric, J-C Sigoillot, J-H Ferrasse
The competitiveness of the second-generation bioethanol by biotechnological process requires an effective and quantitative control of biochemical reactions. In this study, the potential of isothermal calorimetry technique to measure heat and kinetics of a non-homogeneous substrate enzymatic hydrolysis is intended. Using this technique, optimum temperature of the enzymes used for lignocellulosic molecules hydrolysis was determined. Thus, the amount of substrate-to-enzyme ratio was highlighted as an important parameter of the hydrolysis yield...
January 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28053668/study-of-traits-and-recalcitrance-reduction-of-field-grown-comt-down-regulated-switchgrass
#11
Mi Li, Yunqiao Pu, Chang Geun Yoo, Erica Gjersing, Stephen R Decker, Crissa Doeppke, Todd Shollenberger, Timothy J Tschaplinski, Nancy L Engle, Robert W Sykes, Mark F Davis, Holly L Baxter, Mitra Mazarei, Chunxiang Fu, Richard A Dixon, Zeng-Yu Wang, C Neal Stewart, Arthur J Ragauskas
BACKGROUND: The native recalcitrance of plants hinders the biomass conversion process using current biorefinery techniques. Down-regulation of the caffeic acid O-methyltransferase (COMT) gene in the lignin biosynthesis pathway of switchgrass reduced the thermochemical and biochemical conversion recalcitrance of biomass. Due to potential environmental influences on lignin biosynthesis and deposition, studying the consequences of physicochemical changes in field-grown plants without pretreatment is essential to evaluate the performance of lignin-altered plants...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28045609/preparation-of-cellulase-concoction-using-differential-adsorption-phenomenon
#12
Sachinkumar Birhade, Mukesh Pednekar, Shilpa Sagwal, Annamma Odaneth, Arvind Lali
Controlled depolymerization of cellulose is essential for the production of valuable cellooligosaccharides and cellobiose from lignocellulosic biomass. However, enzymatic cellulose hydrolysis involves multiple synergistically acting enzymes, making difficult to control the depolymerization process and generate desired product. This work exploits the varying adsorption properties of the cellulase components to the cellulosic substrate and aims to control the enzyme activity. Cellulase adsorption was favored on pretreated cellulosic biomass as compared to synthetic cellulose...
January 3, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28039140/the-loop-3-of-fungal-endoglucanases-of-glycoside-hydrolase-family-12-modulates-the-catalytic-efficiency
#13
Hong Yang, Pengjun Shi, Yun Liu, Wei Xia, Xiaoyu Wang, Huifang Cao, Rui Ma, Huiying Luo, Yingguo Bai, Bin Yao
: The GH family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. However, the important roles of loop regions of GH12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. This study examined how the loop 3 region affects the enzymatic properties of GH12 glucanases using NfEG12A from Neosartorya fischeri P1 and EG (PDB: 1KS4) from Aspergillus niger Acidophilic and thermophilic NfEG12A had the highest catalytic efficiency (kcat/Km, 3001 and 263 mL/mg/s towards lichenin and CMC-Na, respectively) known so far...
December 30, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28013139/using-polyvinylpyrrolidone-to-enhance-the-enzymatic-hydrolysis-of-lignocelluloses-by-reducing-the-cellulase-non-productive-adsorption-on-lignin
#14
Cheng Cai, Xueqing Qiu, Meijun Zeng, Meilu Lin, Xuliang Lin, Hongming Lou, Xuejuan Zhan, Yuxia Pang, Jinhao Huang, Lingshan Xie
Polyvinylpyrrolidone (PVP) is an antifouling polymer to resist the adsorption of protein on solid surface. Effects of PVP on the enzymatic hydrolysis of pretreated lignocelluloses and its mechanism were studied. Adding 1g/L of PVP8000, the enzymatic digestibility of eucalyptus pretreated by dilute acid (Eu-DA) was increased from 28.9% to 73.4%, which is stronger than the classic additives, such as PEG, Tween and bovine serum albumin. Compared with PEG4600, the adsorption of PVP8000 on lignin was larger, and the adsorption layer was more stable and hydrophilic...
March 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28013134/production-of-a-generic-microbial-feedstock-for-lignocellulose-biorefineries-through-sequential-bioprocessing
#15
Chen-Wei Chang, Colin Webb
Lignocellulosic materials, mostly from agricultural and forestry residues, provide a potential renewable resource for sustainable biorefineries. Reducing sugars can be produced only after a pre-treatment stage, which normally involves chemicals but can be biological. In this case, two steps are usually necessary: solid-state cultivation of fungi for deconstruction, followed by enzymatic hydrolysis using cellulolytic enzymes. In this research, the utilisation of solid-state bioprocessing using the fungus Trichoderma longibrachiatum was implemented as a simultaneous microbial pretreatment and in-situ enzyme production method for fungal autolysis and further enzyme hydrolysis of fermented solids...
March 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28005227/in-planta-production-and-characterization-of-a-hyperthermostable-gh10-xylanase-in-transgenic-sugarcane
#16
Jae Yoon Kim, Guang Nong, John D Rice, Maria Gallo, James F Preston, Fredy Altpeter
Sugarcane (Saccharum sp. hybrids) is one of the most efficient and sustainable feedstocks for commercial production of fuel ethanol. Recent efforts focus on the integration of first and second generation bioethanol conversion technologies for sugarcane to increase biofuel yields. This integrated process will utilize both the cell wall bound sugars of the abundant lignocellulosic sugarcane residues in addition to the sucrose from stem internodes. Enzymatic hydrolysis of lignocellulosic biomass into its component sugars requires significant amounts of cell wall degrading enzymes...
December 22, 2016: Plant Molecular Biology
https://www.readbyqxmd.com/read/27997076/biological-pretreatment-of-corn-stover-with-phlebia-brevispora-nrrl-13108-for-enhanced-enzymatic-hydrolysis-and-efficient-ethanol-production
#17
Badal C Saha, Gregory J Kennedy, Nasib Qureshi, Michael A Cotta
Biological pretreatment of lignocellulosic biomass by white-rot fungus can represent a low-cost and eco-friendly alternative to harsh physical, chemical or physico-chemical pretreatment methods to facilitate enzymatic hydrolysis. In this work, solid state cultivation of corn stover with Phlebia brevispora NRRL-13018 was optimized with respect to duration, moisture content and inoculum size. Changes in composition of pretreated corn stover and its susceptibility to enzymatic hydrolysis were analyzed. About 84% moisture and 42 days incubation at 28°C were found to be optimal for pretreatment with respect to enzymatic saccharification...
December 20, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27987914/effects-of-dilute-acid-and-flowthrough-pretreatments-and-bsa-supplementation-on-enzymatic-deconstruction-of-poplar-by-cellulase-and-xylanase
#18
Samarthya Bhagia, Rajeev Kumar, Charles E Wyman
To help understand factors controlling the recalcitrance of lignocellulosic biomass to deconstruction to sugars, poplar was pretreated with liquid hot water (LHW) and extremely dilute acid (EDA) at 140°C and 180°C in batch and flowthrough reactors. The resulting solids were then subjected to enzymatic hydrolysis by eight combinations of cellulase, xylanase, and bovine serum albumin (BSA). Co-addition of xylanase to cellulase resulted in up to 11 percentage points higher overall sugar yield than their sequential addition...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27919544/dual-effect-of-soluble-materials-in-pretreated-lignocellulose-on-simultaneous-saccharification-and-co-fermentation-process-for-the-bioethanol-production
#19
Lei Qin, Xia Li, Li Liu, Jia-Qing Zhu, Qi-Man Guan, Man-Tong Zhang, Wen-Chao Li, Bing-Zhi Li, Ying-Jin Yuan
In this study, wash liquors isolated from ethylenediamine and dry dilute acid pretreated corn stover were used to evaluate the effect of soluble materials in pretreated biomass on simultaneous saccharification and co-fermentation (SSCF) for ethanol production, respectively. Both of the wash liquors had different impacts on enzymatic hydrolysis and fermentation. Enzymatic conversions of glucan and xylan monotonically decreased as wash liquor concentration increased. Whereas, with low wash liquor concentrations, xylose consumption rate, cell viability and ethanol yield were maximally stimulated in fermentation without nutrient supplementary...
January 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27917379/current-understanding-of-the-correlation-of-lignin-structure-with-biomass-recalcitrance
#20
REVIEW
Mi Li, Yunqiao Pu, Arthur J Ragauskas
Lignin, a complex aromatic polymer in terrestrial plants, contributes significantly to biomass recalcitrance to microbial and/or enzymatic deconstruction. To reduce biomass recalcitrance, substantial endeavors have been exerted on pretreatment and lignin engineering in the past few decades. Lignin removal and/or alteration of lignin structure have been shown to result in reduced biomass recalcitrance with improved cell wall digestibility. While high lignin content is usually a barrier to a cost-efficient application of bioresources to biofuels, the direct correlation of lignin structure and its concomitant properties with biomass remains unclear due to the complexity of cell wall and lignin structure...
2016: Frontiers in Chemistry
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