keyword
MENU ▼
Read by QxMD icon Read
search

Lignocellulosic enzymatic hydrolysis

keyword
https://www.readbyqxmd.com/read/28437478/metal-accumulation-by-sunflower-helianthus-annuus-l-and-the-efficacy-of-its-biomass-in-enzymatic-saccharification
#1
Saurabh Sudha Dhiman, Xin Zhao, Jinglin Li, Dongwook Kim, Vipin C Kalia, In-Won Kim, Jae Young Kim, Jung-Kul Lee
Accumulation of metal contaminants in soil as a result of various industrial and anthropogenic activities has reduced soil fertility significantly. Phytoextraction of metal contaminants can improve soil fertility and provide inexpensive feedstock for biorefineries. We investigated the hyperaccumulation capacity of sunflower (Helianthus annuus) biomass by cultivating these plants in various concentrations of metal contaminants. Sunflowers were grown in soils contaminated with various levels of heavy metals (10-2,000 mg/kg dry soil)...
2017: PloS One
https://www.readbyqxmd.com/read/28433190/evaluation-of-the-effects-of-isolated-lignin-on-enzymatic-hydrolysis-of-cellulose
#2
Hongdan Zhang, Shubin Wu, Jun Xie
The different physical and chemical properties of lignin might have various effects on the enzymatic hydrolysis of lignocellulosic substrates. In this study, the influence of lignin on enzymatic digestibility of cellulose was assessed. Addition of 20% (4g/L) isolated enzymatic lignin (lignin 2 and 3) and kraft lignin (lignin 4) resulted in 5-20% drop of glucose yield, depending on lignin sources. The inhibitory effect of lignin was abated as the enzyme loading increased from 10 to 20FPU/g dry substrate. However, the increasing lignin amount to 40% (8g/L) did not appear to further decrease the cellulose hydrolysis efficiency...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28428822/in-muro-deacetylation-of-xylan-affects-lignin-properties-and-improves-saccharification-of-aspen-wood
#3
Prashant Mohan-Anupama Pawar, Marta Derba-Maceluch, Sun-Li Chong, Madhavi Latha Gandla, Shamrat Shafiul Bashar, Tobias Sparrman, Patrik Ahvenainen, Mattias Hedenström, Merve Özparpucu, Markus Rüggeberg, Ritva Serimaa, Martin Lawoko, Maija Tenkanen, Leif J Jönsson, Ewa J Mellerowicz
BACKGROUND: Lignocellulose from fast growing hardwood species is a preferred source of polysaccharides for advanced biofuels and "green" chemicals. However, the extensive acetylation of hardwood xylan hinders lignocellulose saccharification by obstructing enzymatic xylan hydrolysis and causing inhibitory acetic acid concentrations during microbial sugar fermentation. To optimize lignocellulose for cost-effective saccharification and biofuel production, an acetyl xylan esterase AnAXE1 from Aspergillus niger was introduced into aspen and targeted to cell walls...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28420406/genomics-insights-into-different-cellobiose-hydrolysis-activities-in-two-trichoderma-hamatum-strains
#4
Peng Cheng, Bo Liu, Yi Su, Yao Hu, Yahui Hong, Xinxin Yi, Lei Chen, Shengying Su, Jeffrey S C Chu, Nansheng Chen, Xingyao Xiong
BACKGROUND: Efficient biomass bioconversion is a promising solution to alternative energy resources and environmental issues associated with lignocellulosic wastes. The Trichoderma species of cellulolytic fungi have strong cellulose-degrading capability, and their cellulase systems have been extensively studied. Currently, a major limitation of Trichoderma strains is their low production of β-glucosidases. RESULTS: We isolated two Trichoderma hamatum strains YYH13 and YYH16 with drastically different cellulose degrading efficiencies...
April 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28406925/characterization-of-a-thermophilic-cellulase-from-geobacillus-sp-hta426-an-efficient-cellulase-producer-on-alkali-pretreated-of-lignocellulosic-biomass
#5
Laddawan Potprommanee, Xiao-Qin Wang, Ye-Ju Han, Didonc Nyobe, Yen-Ping Peng, Qing Huang, Jing-Yong Liu, Yu-Ling Liao, Ken-Lin Chang
A themophilic cellulase-producing bacterium was isolated from a hot spring district and identified as Geobacillus sp. HTA426. The cellulase enzyme produced by the Geobacillus sp. HTA426 was purified through ammonium sulfate precipitation and ion exchange chromatography, with the recovery yield and fold purification of 10.14% and 5.12, respectively. The purified cellulase has a molecular weight of 40 kDa. The optimum temperature and pH for carboxymethyl cellulase (CMCase) activity of the purified cellulase were 60°C and pH 7...
2017: PloS One
https://www.readbyqxmd.com/read/28405217/bioprocessing-of-tea-oil-fruit-hull-with-acetic-acid-organosolv-pretreatment-in-combination-with-alkaline-h2o2
#6
Song Tang, Rukuan Liu, Fubao Fuelbiol Sun, Chunying Dong, Rui Wang, Zhongyuan Gao, Zhanying Zhang, Zhihong Xiao, Changzhu Li, Hui Li
BACKGROUND: As a natural renewable biomass, the tea oil fruit hull (TOFH) mainly consists of lignocellulose, together with some bioactive substances. Our earlier work constructed a two-stage solvent-based process, including one aqueous ethanol organosolv extraction and an atmospheric glycerol organosolv (AGO) pretreatment, for bioprocessing of the TOFH into diverse bioproducts. However, the AGO pretreatment is not as selective as expected in removing the lignin from TOFH, resulting in the limited delignification and simultaneously high cellulose loss...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28400859/precise-pretreatment-of-lignocellulose-relating-substrate-modification-with-subsequent-hydrolysis-and-fermentation-to-products-and-by-products
#7
Fan Lü, Lina Chai, Liming Shao, Pinjing He
BACKGROUND: Pretreatment is a crucial step for valorization of lignocellulosic biomass into valuable products such as H2, ethanol, acids, and methane. As pretreatment can change several decisive factors concurrently, it is difficult to predict its effectiveness. Furthermore, the effectiveness of pretreatments is usually assessed by enzymatic digestibility or merely according to the yield of the target fermentation products. The present study proposed the concept of "precise pretreatment," distinguished the major decisive factors of lignocellulosic materials by precise pretreatment, and evaluated the complete profile of all fermentation products and by-products...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28400170/enhanced-simultaneous-saccharification-and-fermentation-of-pretreated-beech-wood-by-in-situ-treatment-with-the-white-rot-fungus-irpex-lacteus-in-a-membrane-aerated-biofilm-reactor
#8
Simone Brethauer, Shahab Robert Lawrence, Studer Michael Hans-Peter
The aim of the present study was to investigate the combination of steam pretreatment and biological treatment with lignin degrading fungal strains in order to enable efficient bioprocessing of beech wood to ethanol. In a sequential process of steam and fungal pretreatment followed by enzymatic hydrolysis, Irpex lacteus almost doubled the glucose yield for mildly pretreated beech wood, but could not improve yields for more severely pretreated substrates. However, when simultaneous saccharification and fermentation is combined with in situ I...
March 18, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28388525/development-of-combined-nanofiltration-and-forward-osmosis-process-for-production-of-ethanol-from-pretreated-rice-straw
#9
Masafumi Shibuya, Kengo Sasaki, Yasuhiro Tanaka, Masahiro Yasukawa, Tomoki Takahashi, Akihiko Kondo, Hideto Matsuyama
A membrane process combining nanofiltraion (NF) and forward osmosis (FO) was developed for the sugar concentration with the aim of high bio-ethanol production from the liquid fraction of rice straw. The commercial NF membrane, ESNA3, was more adequate for removal of fermentation inhibitors (such as acetic acid) than the FO membrane, whereas the commercial FO membrane, TFC-ES, was more adequate for concentration of the sugars than the NF membrane. The liquid fraction was subjected to the following process: NF concentration with water addition (NF(+H2O))→enzymatic hydrolysis→FO concentration...
March 28, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28386674/microplate-based-evaluation-of-the-sugar-yield-from-giant-reed-giant-miscanthus-and-switchgrass-after-mild-chemical-pre-treatments-and-hydrolysis-with-tailored-trichoderma-enzymatic-blends
#10
Stefano Cianchetta, Luca Bregoli, Stefania Galletti
Giant reed, miscanthus, and switchgrass are considered prominent lignocellulosic feedstocks to obtain fermentable sugars for biofuel production. The bioconversion into sugars requires a delignifying pre-treatment step followed by hydrolysis with cellulase and other accessory enzymes like xylanase, especially in the case of alkali pre-treatments, which retain the hemicellulose fraction. Blends richer in accessory enzymes than commercial mix can be obtained growing fungi on feedstock-based substrates, thus ten selected Trichoderma isolates, including the hypercellulolytic strain Trichoderma reesei Rut-C30, were grown on giant reed, miscanthus, or switchgrass-based substrates...
April 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28385212/synergistic-surfactant-assisted-emim-oac-pretreatment-of-lignocellulosic-waste-for-enhanced-cellulose-accessibility-to-cellulase
#11
Amir Goshadrou, Mark Lefsrud
The present study investigated for the first time the efficacy of surfactant-assisted ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc) pretreatment of beech wood waste for enhanced ethanol production. The ionic liquid (IL) pretreatment included wood dissolution at 120°C and 5% solid loading for 3h followed by regeneration using water as an anti-solvent. The substrate was initially presoaked in different concentrations of surfactant solution (2%, 4%, 6% w/w) prior to IL pretreatment. Tween 20, Tween 80 and polyethylene glycol 4000 (PEG) surfactants were tested and the results revealed major improvements in the yield of enzymatic hydrolysis...
June 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28384075/synergistic-action-between-extracellular-products-from-white-rot-fungus-and-cellulase-significantly-improves-enzymatic-hydrolysis
#12
Yushan Wang, Yang Shao, Xinyue Zou, Mandi Yang, Lin Guo
With a set of perfect extracellular lignocellulolytic enzymes, white-rot fungus has been recognized as playing an important role in the degradation of lignocellulose materials, which leads to the possibility of creating a composite enzymatic system with high hydrolysis efficiency in vitro. Echinodontium taxodii is a promising white-rot fungus for biological pretreatment. In this study, we extracted the extracellular products of E. taxodii under solid-state fermentation conditions, mixed the extracellular products with cellulase to build a composite enzymatic system, and systematically evaluated the effect of this system on the hydrolysis of acid-pretreated and raw corn stovers...
April 6, 2017: Bioengineered
https://www.readbyqxmd.com/read/28369757/development-of-rapid-bioconversion-with-integrated-recycle-technology-for-ethanol-production-from-extractive-ammonia-pretreated-corn-stover
#13
Mingjie Jin, Yanping Liu, Leonardo da Costa Sousa, Bruce E Dale, Venkatesh Balan
High enzyme loading and low productivity are two major issues impeding low cost ethanol production from lignocellulosic biomass. This work applied Rapid Bioconversion with Integrated recycle Technology (RaBIT) and Extractive Ammonia (EA) pretreatment for conversion of corn stover (CS) to ethanol at high solids loading. Enzymes were recycled via recycling unhydrolyzed solids. Enzymatic hydrolysis with recycled enzymes and fermentation with recycled yeast cells were studied. Both enzymatic hydrolysis time and fermentation time were shortened to 24 h...
April 1, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28363656/structural-variations-of-lignin-macromolecule-from-different-growth-years-of-triploid-of-populus-tomentosa-carr
#14
Tian-Ying Chen, Bing Wang, Yu-Ying Wu, Jia-Long Wen, Chuan-Fu Liu, Tong-Qi Yuan, Run-Cang Sun
To better understand the variations of structural characteristics of lignin macromolecules during different growth years of Triploid of Populus tomentosa Carr, a novel lignin isolation procedure based on double ball-milling and enzymatic hydrolysis (DEL) was proposed in this study. The morphological distributions of lignin in the plant cell wall of these poplar wood samples were monitored by Confocal Raman Microscopy (CRM). The ultrahigh yields (105.1%-111.2%) of DELs were significantly higher than those (24...
March 29, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28360940/comprehensive-characterization-of-non-cellulosic-recalcitrant-cell-wall-carbohydrates-in-unhydrolyzed-solids-from-afex-pretreated-corn-stover
#15
Christa Gunawan, Saisi Xue, Sivakumar Pattathil, Leonardo da Costa Sousa, Bruce E Dale, Venkatesh Balan
BACKGROUND: Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments. Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass). Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28351654/lignin-enzyme-interaction-mechanism-mitigation-approach-modeling-and-research-prospects
#16
REVIEW
Xiang Li, Yi Zheng
The adverse environmental impacts of the fossil fuel and the concerns of energy security necessitate the development of alternative clean energy sources from renewable feedstocks. Lignocellulosic biomass is a 2nd generation feedstock used in the production of biofuels and bio-based products that are conventionally derived from fossil resources. The biochemical conversion, which entails biomass pretreatment, enzymatic hydrolysis and fermentation, is one major platform used to transform lignocelluloses into biofuels...
March 25, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28350993/convergent-strategy-for-the-synthesis-of-s-linked-oligoxylans
#17
Beatrice Bonora, Irene Boos, Mads H Clausen
Arabinoxylans (AX) are a major class of hemicellulose and an important polysaccharide component of lignocellulosic biomass. To utilize the glycan polymer effectively, it is desirable to learn more about the enzymatic hydrolysis of AXs. Well-defined glycans can help to elucidate these processes. Here, we report the efficient synthesis of a mixed O- and S-linked tetraxylan. This thio-oligosaccharide has been developed as a putative inhibitor of arabinoxylan degrading enzymes used for the saccharification of biomass...
March 21, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28330211/cellulase-production-using-natural-medium-and-its-application-on-enzymatic-hydrolysis-of-thermo-chemically-pretreated-biomass
#18
Shivani Sharma, Vinay Sharma, Arindam Kuila
Lignocellulosic bioethanol is an important renewable fuel for transportation purpose. Commercial production of lignocellulosic bioethanol mainly depends on cost of cellulase production, efficient pretreatment and enzymatic hydrolysis process. In the present study cellulase production from Aspergillus niger under submerged fermentation (SmF) was optimized using coconut water as natural medium. Maximum cellulase production (0.53 IU/mL) was achieved within 3 days of incubation using 8 % (w/v) waste paper and 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330203/evaluation-of-several-ionic-liquids-for-in-situ-hydrolysis-of-empty-fruit-bunches-by-locally-produced-cellulase
#19
Amal Ahmed Elgharbawy, Md Zahangir Alam, Nassereldeen Ahmad Kabbashi, Muhammad Moniruzzaman, Parveen Jamal
Lignocellulosic biomasses, exhibit resistance to enzymatic hydrolysis due to the presence of lignin and hemicellulose. Ionic liquids proved their applicability in lignin degradation, however, ionic liquid removal has to be performed to proceed to hydrolysis. Therefore, this study reports an in situ hydrolysis of empty fruit bunches (EFB) that combined an ionic liquid (IL) pretreatment and enzymatic hydrolysis. For enzyme production, palm kernel cake (PKC) was used as the primary media for microbial cellulase (PKC-Cel) from Trichoderma reesei (RUTC30)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330198/pretreatment-and-enzymatic-process-modification-strategies-to-improve-efficiency-of-sugar-production-from-sugarcane-bagasse
#20
Siddhartha Pal, Shereena Joy, Kalpana D Trimukhe, Pramod S Kumbhar, Anjani J Varma, Sasisanker Padmanabhan
Pretreatment and enzymatic hydrolysis play a critical role in the economic production of sugars and fuels from lignocellulosic biomass. In this study, we evaluated diverse pilot-scale pretreatments and different post-pretreatment strategies for the production of fermentable sugars from sugarcane bagasse. For the pretreatment of bagasse at pilot-scale level, steam explosion without catalyst and combination of sulfuric and oxalic acids at low and high loadings were used. Subsequently, to enhance the efficiency of enzymatic hydrolysis of the pretreated bagasse, three different post-pretreatment process schemes were investigated...
December 2016: 3 Biotech
keyword
keyword
42103
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"