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Lignocellulosic enzymatic hydrolysis

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https://www.readbyqxmd.com/read/28535986/intracellular-cellobiose-metabolism-and-its-applications-in-lignocellulose-based-biorefineries
#1
REVIEW
Vinuselvi Parisutham, Sathesh-Prabu Chandran, Aindrila Mukhopadhyay, Sung Kuk Lee, Jay D Keasling
Complete hydrolysis of cellulose has been a key characteristic of biomass technology because of the limitation of industrial production hosts to use cellodextrin, the partial hydrolysis product of cellulose. Cellobiose, a β-1,4-linked glucose dimer, is a major cellodextrin of the enzymatic hydrolysis (via endoglucanase and exoglucanase) of cellulose. Conversion of cellobiose to glucose is executed by β-glucosidase. The complete extracellular hydrolysis of celluloses has several critical barriers in biomass technology...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28529543/pilot-scale-steam-explosion-pretreatment-with-2-naphthol-to-overcome-high-softwood-recalcitrance
#2
Thomas Pielhop, Janick Amgarten, Michael H Studer, Philipp Rudolf von Rohr
BACKGROUND: Steam explosion pretreatment has been examined in many studies for enhancing the enzymatic digestibility of lignocellulosic biomass and is currently the most common pretreatment method in commercial biorefineries. It is however not effective for overcoming the extremely high recalcitrance of softwood to biochemical conversion. Recent fundamental research in small-scale liquid hot water pretreatment has shown, though, that the addition of a carbocation scavenger like 2-naphthol can prevent lignin repolymerization and thus enhance the enzymatic digestibility of softwood cellulose...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28515785/recombinant-expression-of-thermostable-processive-mteg5-endoglucanase-and-its-synergism-with-mtlpmo-from-myceliophthora-thermophila-during-the-hydrolysis-of-lignocellulosic-substrates
#3
Anthi Karnaouri, Madhu Nair Muraleedharan, Maria Dimarogona, Evangelos Topakas, Ulrika Rova, Mats Sandgren, Paul Christakopoulos
BACKGROUND: Filamentous fungi are among the most powerful cellulolytic organisms in terrestrial ecosystems. To perform the degradation of lignocellulosic substrates, these microorganisms employ both hydrolytic and oxidative mechanisms that involve the secretion and synergism of a wide variety of enzymes. Interactions between these enzymes occur on the level of saccharification, i.e., the release of neutral and oxidized products, but sometimes also reflected in the substrate liquefaction...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28500891/steam-exploded-biomass-saccharification-is-predominately-affected-by-lignocellulose-porosity-and-largely-enhanced-by-tween-80-in-miscanthus
#4
Dan Sun, Aftab Alam, Yuanyuan Tu, Shiguang Zhou, Yanting Wang, Tao Xia, Jiangfeng Huang, Ying Li, Zahoor, Xiaoyang Wei, Bo Hao, Liangcai Peng
In this study, total ten Miscanthus accessions exhibited diverse cell wall compositions, leading to largely varied hexoses yields at 17%-40% (% cellulose) released from direct enzymatic hydrolysis of steam-exploded (SE) residues. Further supplied with 2% Tween-80 into the enzymatic digestion, the Mis7 accession showed the higher hexose yield by 14.8-fold than that of raw material, whereas the Mis10 had the highest hexoses yield at 77% among ten Miscanthus accessions. Significantly, this study identified four wall polymer features that negatively affect biomass saccharification as p<0...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28482283/using-a-combined-hydrolysis-factor-to-balance-enzymatic-saccharification-and-the-structural-characteristics-of-lignin-during-pretreatment-of-hybrid-poplar-with-a-fully-recyclable-solid-acid
#5
Hairui Ji, Yanliang Song, Xu Zhang, Tianwei Tan
In this study, a new pretreatment strategy for lignocellulosic was developed using a fully recyclable solid acid, Toluenesulfonic acid (p-TsOH). A combined hydrolysis factor (CHF) as a pretreatment severity was used to balance enzymatic saccharification and the structural characteristics of lignin. The results from degradation of carbohydrates, enzymatic hydrolysis of cellulose and characterization of lignin by FT-IR, (31)P NMR, GPC, 2D-HSQC NMR indicated that a CHF of approximately 3.90 was the optimal pretreatment severity to facilitate enzymatic saccharification and the potential serviceability of lignin...
April 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28474425/ethanol-production-from-dilute-acid-steam-exploded-lignocellulosic-feedstocks-using-an-isolated-multistress-tolerant-pichia-kudriavzevii-strain
#6
Shuo-Fu Yuan, Gia-Luen Guo, Wen-Song Hwang
Renewable and low-cost lignocellulosic wastes have attractive applications in bioethanol production. The yeast Saccharomyces cerevisiae is the most widely used ethanol-producing microbe; however, its fermentation temperature (30-35°C) is not optimum (40-50°C) for enzymatic hydrolysis in the simultaneous saccharification and fermentation (SSF) process. In this study, we successfully performed an SSF process at 42°C from a high solid loading of 20% (w/v) acid-impregnated steam explosion (AISE)-treated rice straw with low inhibitor concentrations (furfural 0...
May 5, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28469707/characterisation-of-non-degraded-oligosaccharides-in-enzymatically-hydrolysed-and-fermented-dilute-ammonia-pretreated-corn-stover-for-ethanol-production
#7
M C Jonathan, J DeMartini, S Van Stigt Thans, R Hommes, M A Kabel
BACKGROUND: Corn stover is lignocellulosic biomass that has potential to be used as raw material for bioethanol production. In the current research, dilute ammonia pretreatment was used to improve the accessibility of corn stover carbohydrates to subsequently added hydrolytic enzymes. Some carbohydrates, however, were still present after enzymatic hydrolysis and fermentation. Hence, this research was aimed to characterise the recalcitrant carbohydrates, especially the oligosaccharides that remained after hydrolysis and fermentation of dilute ammonia-pretreated corn stover (DACS)...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28456045/hydrothermal-pretreatment-and-enzymatic-hydrolysis-of-mixed-green-and-woody-lignocellulosics-from-arid-regions
#8
Muhammad Tahir Ashraf, Mette Hedegaard Thomsen, Jens Ejbye Schmidt
Utilization of multi-specie feedstocks is imperative for application of lignocellulosic biorefineries in arid regions. Different lignocellulosic residues vary in composition and anatomical features. Pretreatment and enzymatic hydrolysis are two processes at the front end of any lignocellulosics biorefinery applying biochemical pathway, and have to efficiently deal with the variance in the feedstock composition and properties. However, there is limited knowledge about effect of mixing different lignocellulosics on pretreatment and enzymatic hydrolysis yields...
April 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28450886/moderate-alkali-thermophilic-ethanologenesis-by-locally-isolated-bacillus-licheniformis-from-pakistan-employing-sugarcane-bagasse-a-comparative-aspect-of-aseptic-and-non-aseptic-fermentations
#9
Qurat-Ul-Ain Ahmad, Shang-Tian Yang, Maleeha Manzoor, Javed Iqbal Qazi
BACKGROUND: Biofuels obtained from first-generation (1G) sugars-starch streams have been proven unsustainable as their constant consumption is not only significantly costly for commercial scale production systems, but it could potentially lead to problems associated with extortionate food items for human usage. In this regard, biofuels' production in alkali-thermophilic environs from second-generation (2G) bio-waste would not only be markedly feasible, but these extreme conditions might be able to sustain aseptic fermentations without spending much for sterilization...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28444599/study-of-enzymatic-saccharification-of-agave-leaves-biomass-to-yield-fermentable-sugars
#10
Miguel A Medina-Morales, Oscar Soto-Cruz, Juan C Contreras-Esquivel, Raúl Rodríguez-Herrera, Heliodoro De la Garza-Toledo, Cristóbal N Aguilar
Agave is a good source of polysaccharides for the production of fermentable sugars as sustainable bioenergy feedstock solutions for semi-arid and arid lands. This plant grows in arid areas, which correspond to a large territory in northern Mexico. Having lignocellulose as the polysaccharide of interest, the information for the enzymatic saccharification of this kind of material is limited. Agave cell walls have a unique recalcitrant nature, but having a high cellulose content, makes this plant material an interesting research subject...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444598/synergistic-effect-of-thermostable-%C3%AE-glucosidase-tn0602-and-cellulase-on-cellulose-hydrolysis
#11
Zhaoye Zhang, Man Wang, Renjun Gao, Xiaoxiao Yu, Guang Chen
Thermophilic enzymes have many potential benefits in industrial production with increased flexibility related to process configurations. A thermostable β-glucosidase from Thermotoga naphthophila RUK-10 was found to possess catalytic activity for cellobiose hydrolysis with a high potential for application in biomass conversion. The aggregation of cellobiose often has an inhibitory effect on cellobiohydrolases and endoglucanases during cellulose hydrolysis. The presence of β-glucosidases has a significant effect on reducing inhibition from hydrolytic products by hydrolysing the intermedia cellobiose...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28437478/metal-accumulation-by-sunflower-helianthus-annuus-l-and-the-efficacy-of-its-biomass-in-enzymatic-saccharification
#12
Saurabh Sudha Dhiman, Xin Zhao, Jinglin Li, Dongwook Kim, Vipin C Kalia, In-Won Kim, Jae Young Kim, Jung-Kul Lee
Accumulation of metal contaminants in soil as a result of various industrial and anthropogenic activities has reduced soil fertility significantly. Phytoextraction of metal contaminants can improve soil fertility and provide inexpensive feedstock for biorefineries. We investigated the hyperaccumulation capacity of sunflower (Helianthus annuus) biomass by cultivating these plants in various concentrations of metal contaminants. Sunflowers were grown in soils contaminated with various levels of heavy metals (10-2,000 mg/kg dry soil)...
2017: PloS One
https://www.readbyqxmd.com/read/28433190/evaluation-of-the-effects-of-isolated-lignin-on-enzymatic-hydrolysis-of-cellulose
#13
Hongdan Zhang, Shubin Wu, Jun Xie
The different physical and chemical properties of lignin might have various effects on the enzymatic hydrolysis of lignocellulosic substrates. In this study, the influence of lignin on enzymatic digestibility of cellulose was assessed. Addition of 20% (4g/L) isolated enzymatic lignin (lignin 2 and 3) and kraft lignin (lignin 4) resulted in 5-20% drop of glucose yield, depending on lignin sources. The inhibitory effect of lignin was abated as the enzyme loading increased from 10 to 20FPU/g dry substrate. However, the increasing lignin amount to 40% (8g/L) did not appear to further decrease the cellulose hydrolysis efficiency...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28428822/in-muro-deacetylation-of-xylan-affects-lignin-properties-and-improves-saccharification-of-aspen-wood
#14
Prashant Mohan-Anupama Pawar, Marta Derba-Maceluch, Sun-Li Chong, Madhavi Latha Gandla, Shamrat Shafiul Bashar, Tobias Sparrman, Patrik Ahvenainen, Mattias Hedenström, Merve Özparpucu, Markus Rüggeberg, Ritva Serimaa, Martin Lawoko, Maija Tenkanen, Leif J Jönsson, Ewa J Mellerowicz
BACKGROUND: Lignocellulose from fast growing hardwood species is a preferred source of polysaccharides for advanced biofuels and "green" chemicals. However, the extensive acetylation of hardwood xylan hinders lignocellulose saccharification by obstructing enzymatic xylan hydrolysis and causing inhibitory acetic acid concentrations during microbial sugar fermentation. To optimize lignocellulose for cost-effective saccharification and biofuel production, an acetyl xylan esterase AnAXE1 from Aspergillus niger was introduced into aspen and targeted to cell walls...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28420406/genomics-insights-into-different-cellobiose-hydrolysis-activities-in-two-trichoderma-hamatum-strains
#15
Peng Cheng, Bo Liu, Yi Su, Yao Hu, Yahui Hong, Xinxin Yi, Lei Chen, Shengying Su, Jeffrey S C Chu, Nansheng Chen, Xingyao Xiong
BACKGROUND: Efficient biomass bioconversion is a promising solution to alternative energy resources and environmental issues associated with lignocellulosic wastes. The Trichoderma species of cellulolytic fungi have strong cellulose-degrading capability, and their cellulase systems have been extensively studied. Currently, a major limitation of Trichoderma strains is their low production of β-glucosidases. RESULTS: We isolated two Trichoderma hamatum strains YYH13 and YYH16 with drastically different cellulose degrading efficiencies...
April 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28406925/characterization-of-a-thermophilic-cellulase-from-geobacillus-sp-hta426-an-efficient-cellulase-producer-on-alkali-pretreated-of-lignocellulosic-biomass
#16
Laddawan Potprommanee, Xiao-Qin Wang, Ye-Ju Han, Didonc Nyobe, Yen-Ping Peng, Qing Huang, Jing-Yong Liu, Yu-Ling Liao, Ken-Lin Chang
A themophilic cellulase-producing bacterium was isolated from a hot spring district and identified as Geobacillus sp. HTA426. The cellulase enzyme produced by the Geobacillus sp. HTA426 was purified through ammonium sulfate precipitation and ion exchange chromatography, with the recovery yield and fold purification of 10.14% and 5.12, respectively. The purified cellulase has a molecular weight of 40 kDa. The optimum temperature and pH for carboxymethyl cellulase (CMCase) activity of the purified cellulase were 60°C and pH 7...
2017: PloS One
https://www.readbyqxmd.com/read/28405217/bioprocessing-of-tea-oil-fruit-hull-with-acetic-acid-organosolv-pretreatment-in-combination-with-alkaline-h2o2
#17
Song Tang, Rukuan Liu, Fubao Fuelbiol Sun, Chunying Dong, Rui Wang, Zhongyuan Gao, Zhanying Zhang, Zhihong Xiao, Changzhu Li, Hui Li
BACKGROUND: As a natural renewable biomass, the tea oil fruit hull (TOFH) mainly consists of lignocellulose, together with some bioactive substances. Our earlier work constructed a two-stage solvent-based process, including one aqueous ethanol organosolv extraction and an atmospheric glycerol organosolv (AGO) pretreatment, for bioprocessing of the TOFH into diverse bioproducts. However, the AGO pretreatment is not as selective as expected in removing the lignin from TOFH, resulting in the limited delignification and simultaneously high cellulose loss...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28400859/precise-pretreatment-of-lignocellulose-relating-substrate-modification-with-subsequent-hydrolysis-and-fermentation-to-products-and-by-products
#18
Fan Lü, Lina Chai, Liming Shao, Pinjing He
BACKGROUND: Pretreatment is a crucial step for valorization of lignocellulosic biomass into valuable products such as H2, ethanol, acids, and methane. As pretreatment can change several decisive factors concurrently, it is difficult to predict its effectiveness. Furthermore, the effectiveness of pretreatments is usually assessed by enzymatic digestibility or merely according to the yield of the target fermentation products. The present study proposed the concept of "precise pretreatment," distinguished the major decisive factors of lignocellulosic materials by precise pretreatment, and evaluated the complete profile of all fermentation products and by-products...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28400170/enhanced-simultaneous-saccharification-and-fermentation-of-pretreated-beech-wood-by-in-situ-treatment-with-the-white-rot-fungus-irpex-lacteus-in-a-membrane-aerated-biofilm-reactor
#19
Simone Brethauer, Shahab Robert Lawrence, Studer Michael Hans-Peter
The aim of the present study was to investigate the combination of steam pretreatment and biological treatment with lignin degrading fungal strains in order to enable efficient bioprocessing of beech wood to ethanol. In a sequential process of steam and fungal pretreatment followed by enzymatic hydrolysis, Irpex lacteus almost doubled the glucose yield for mildly pretreated beech wood, but could not improve yields for more severely pretreated substrates. However, when simultaneous saccharification and fermentation is combined with in situ I...
March 18, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28388525/development-of-combined-nanofiltration-and-forward-osmosis-process-for-production-of-ethanol-from-pretreated-rice-straw
#20
Masafumi Shibuya, Kengo Sasaki, Yasuhiro Tanaka, Masahiro Yasukawa, Tomoki Takahashi, Akihiko Kondo, Hideto Matsuyama
A membrane process combining nanofiltraion (NF) and forward osmosis (FO) was developed for the sugar concentration with the aim of high bio-ethanol production from the liquid fraction of rice straw. The commercial NF membrane, ESNA3, was more adequate for removal of fermentation inhibitors (such as acetic acid) than the FO membrane, whereas the commercial FO membrane, TFC-ES, was more adequate for concentration of the sugars than the NF membrane. The liquid fraction was subjected to the following process: NF concentration with water addition (NF(+H2O))→enzymatic hydrolysis→FO concentration...
March 28, 2017: Bioresource Technology
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