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Lignocellulosic enzymatic hydrolysis

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https://www.readbyqxmd.com/read/28941275/reconstruction-of-lignin-and-hemicelluloses-by-aqueous-ethanol-anti-solvents-to-improve-the-ionic-liquid-acid-pretreatment-performance-of-arundo-donax-linn
#1
Tingting You, Ruizhen Wang, Xueming Zhang, Shri Ramaswamy, Feng Xu
Ionic liquid (IL)-acid pretreatment is known to not only enhance the enzymatic hydrolysis efficiency of lignocellulose but also to generate deposits on the surface of fiber by conventional water regeneration, which retard the increment. In this study, ethanol aqueous solution regeneration was developed as a new method to change the substrates characteristics for IL-acid pretreatment and their effects on the enzymatic hydrolysis were evaluated. Following the IL-acid reaction, the biomass slurry was subjected to ethanol aqueous solution at various concentration...
September 23, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28932978/biobutanol-production-from-apple-pomace-the-importance-of-pretreatment-methods-on-the-fermentability-of-lignocellulosic-agro-food-wastes
#2
María Hijosa-Valsero, Ana I Paniagua-García, Rebeca Díez-Antolínez
Apple pomace was studied as a possible raw material for biobutanol production. Five different soft physicochemical pretreatments (autohydrolysis, acids, alkalis, organic solvents and surfactants) were compared in a high-pressure reactor, whose working parameters (temperature, time and reagent concentration) were optimised to maximise the amount of simple sugars released and to minimise inhibitor generation. The pretreated biomass was subsequently subjected to a conventional enzymatic treatment to complete the hydrolysis...
September 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28932263/how-does-cellulosome-composition-influence-deconstruction-of-lignocellulosic-substrates-in-clostridium-ruminiclostridium-thermocellum-dsm-1313
#3
Shahar Yoav, Yoav Barak, Melina Shamshoum, Ilya Borovok, Raphael Lamed, Bareket Dassa, Yitzhak Hadar, Ely Morag, Edward A Bayer
BACKGROUND: Bioethanol production processes involve enzymatic hydrolysis of pretreated lignocellulosic biomass into fermentable sugars. Due to the relatively high cost of enzyme production, the development of potent and cost-effective cellulolytic cocktails is critical for increasing the cost-effectiveness of bioethanol production. In this context, the multi-protein cellulolytic complex of Clostridium (Ruminiclostridium) thermocellum, the cellulosome, was studied here. C. thermocellum is known to assemble cellulosomes of various subunit (enzyme) compositions, in response to the available carbon source...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28929139/rapid-and-near-complete-dissolution-of-wood-lignin-at-%C3%A2-80%C3%A2-c-by-a-recyclable-acid-hydrotrope
#4
Liheng Chen, Jinze Dou, Qianli Ma, Ning Li, Ruchun Wu, Huiyang Bian, Daniel J Yelle, Tapani Vuorinen, Shiyu Fu, Xuejun Pan, Junyong J Y Zhu
We report the discovery of the hydrotropic properties of a recyclable aromatic acid, p-toluenesulfonic acid (p-TsOH), for potentially low-cost and efficient fractionation of wood through rapid and near-complete dissolution of lignin. Approximately 90% of poplar wood (NE222) lignin can be dissolved at 80°C in 20 min. Equivalent delignification using known hydrotropes, such as aromatic salts, can be achieved only at 150°C or higher for more than 10 hours or at 150°C for 2 hours with alkaline pulping. p-TsOH fractionated wood into two fractions: (i) a primarily cellulose-rich water-insoluble solid fraction that can be used for the production of high-value building blocks, such as dissolving pulp fibers, lignocellulosic nanomaterials, and/or sugars through subsequent enzymatic hydrolysis; and (ii) a spent acid liquor stream containing mainly dissolved lignin that can be easily precipitated as lignin nanoparticles by diluting the spent acid liquor to below the minimal hydrotrope concentration...
September 2017: Science Advances
https://www.readbyqxmd.com/read/28926703/effect-of-non-ionic-surfactants-on-dispersion-and-polar-interactions-in-the-adsorption-of-cellulases-onto-lignin
#5
Feng Jiang, Chen Qian, Alan R Esker, Maren Roman
Residual lignin in pretreated biomass impedes enzymatic hydrolysis. Non-ionic surfactants are known to enhance the enzymatic hydrolysis of lignocellulosic biomass but their mechanisms of action are incompletely understood. This study investigates the effect of a non-ionic surfactant, Tween 80, on the adsorption of cellulases onto model lignin substrates. Lignin substrates were prepared by spin coating of flat substrates with three different types of lignin: organosolv lignin, kraft lignin, and milled wood lignin...
September 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28924453/a-novel-and-efficient-fungal-delignification-strategy-based-on-versatile-peroxidase-for-lignocellulose-bioconversion
#6
Wen Kong, Xiao Fu, Lei Wang, Ahmad Alhujaily, Jingli Zhang, Fuying Ma, Xiaoyu Zhang, Hongbo Yu
BACKGROUND: The selective lignin-degrading white-rot fungi are regarded to be the best lignin degraders and have been widely used for reducing the saccharification recalcitrance of lignocellulose. However, the biological delignification and conversion of lignocellulose in biorefinery is still limited. It is necessary to develop novel and more efficient bio-delignification systems. RESULTS: Physisporinus vitreus relies on a new versatile peroxidase (VP)-based delignification strategy to remove enzymatic recalcitrance of corn stover efficiently, so that saccharification of corn stover was significantly enhanced to 349...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28917109/a-%C3%AE-xylosidase-hyper-production-penicillium-oxalicum-mutant-enhanced-ethanol-production-from-alkali-pretreated-corn-stover
#7
Yanxin Ye, Xuezhi Li, Yuan Cao, Jian Du, Shicheng Chen, Jian Zhao
β-Xylosidase activity is deficient in most cellulase enzymes secreted by filamentous fungi, which limits effective enzymatic hydrolysis of hemicellulose in lignocellulose materials and resulted in accumulation of xylo-oligosaccharides that inhibit the cellulase and xylanase activitives. An endogenous β-xylosidase gene, xyl3A, was overexpressed using two types of promoters in cellulolytic P. oxalicum RE-10. The mutants RXyl, RGXyl-1 and RGXyl-2 displayed higher β-xylosidase production than native strain RE-10 besides higher cellulase and xylanase activities, especially RGXyl-1, showing the highest β-xylosidase activity of 15...
August 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28912429/production-and-characteristics-of-a-novel-xylose-and-alkali-tolerant-gh-43-%C3%AE-xylosidase-from-penicillium-oxalicum-for-promoting-hemicellulose-degradation
#8
Yanxin Ye, Xuezhi Li, Jian Zhao
β-xylosidase is a pivotal enzyme for complete degradation of xylan in hemicelluloses of lignocelluloses, and the xylose- and alkali-tolerant β-xylosidase with high catalytic activity is very attractive for promoting enzymatic hydrolysis of alkaline-pretreated lignocellulose. In this study, a novel intracellular glycoside hydrolase family 43 β-xylosidase gene (xyl43) from Penicillium oxalicum 114-2 was successfully high-level overexpressed in Pichia pastoris, and the secreted enzyme was characterized. The β-xylosidase Xyl43 exhibited great pH stability and high catalytic activity in the range of pH 6...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28904370/in-planta-expression-of-hyperthermophilic-enzymes-as-a-strategy-for-accelerated-lignocellulosic-digestion
#9
Bilal Ahmad Mir, Alexander A Myburg, Eshchar Mizrachi, Don A Cowan
Conversion of lignocellulosic biomass to biofuels and biomaterials suffers from high production costs associated with biomass pretreatment and enzymatic hydrolysis. In-planta expression of lignocellulose-digesting enzymes is a promising approach to reduce these cost elements. However, this approach faces a number of challenges, including auto-hydrolysis of developing cell walls, plant growth and yield penalties, low expression levels and the limited stability of expressed enzymes at the high temperatures generally used for biomass processing to release fermentable sugars...
September 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28901274/revalorizing-lignocellulose-for-the-production-of-natural-pharmaceuticals-and-other-high-value-bioproducts
#10
Heng-Phon Too, Congqiang Zhang
Lignocellulose is the most plentiful, renewable natural resource on earth and has been successfully used for the production of biofuels. A significant challenge is to develop cost-effective, environmentally friendly and efficient processes for the conversion of lignocellulose material into suitable substrates for biotransformation. A number of approaches have been explored to convert lignocellulose into sugars, e.g. combining chemical pretreatment and enzymatic hydrolysis. In nature, there are organisms that can biotransform the complex lignocellulose efficiently, such as wood-degrading fungi (brown rot and white rot fungi), bacteria (e...
September 11, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28866806/potential-of-mangrove-associated-endophytic-fungi-for-production-of-carbohydrolases-with-high-saccharification-efficiency
#11
M M C Maroldi, V M Vasconcellos, P T Lacava, C S Farinas
The endophytic fungi represent a potential source of microorganisms for enzyme production. However, there have been only few studies exploiting their potential for the production of enzymes of industrial interest, such as the (hemi)cellulolytic enzymatic cocktail required in the hydrolysis of lignocellulosic biomass. Here, a collection of endophytic fungi isolated from mangrove tropical forests was evaluated for the production of carbohydrolases and performance on the hydrolysis of cellulose. For that, 41 endophytic strains were initially screened using a plate assay containing crystalline cellulose as the sole carbon source and the selected strains were cultivated under solid-state fermentation for endoglucanase, β-glucosidase, and xylanase enzyme quantification...
September 2, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28864653/chemical-pretreatment-independent-saccharifications-of-xylan-and-cellulose-of-rice-straw-by-bacterial-weak-lignin-binding-xylanolytic-and-cellulolytic-enzymes
#12
Thitiporn Teeravivattanakit, Sirilak Baramee, Paripok Phitsuwan, Somphit Sornyotha, Rattiya Waeonukul, Patthra Pason, Chakrit Tachaapaikoon, Kanokwan Poomputsa, Akihiko Kosugi, Kazuo Sakka, Khanok Ratanakhanokchai
Complete utilization of carbohydrate fractions is one of the prerequisites for obtaining an economically favourable lignocellulosic biomass conversion. This study shows that xylan in untreated rice straw was saccharified to xylose in one step without chemical pretreatment, yielding 58.2% of the theoretically maximum value by Paenibacillus curdlanolyticus B-6 PcAxy43A, a weak lignin-binding trifunctional xylanolytic enzyme, endoxylanase/β-xylosidase/arabinoxylan arabinofuranohydrolase. Moreover, xylose yield from untreated rice straw was enhanced to 78...
September 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28860612/unraveling-variations-of-crystalline-cellulose-induced-by-ionic-liquid-and-their-effects-on-enzymatic-hydrolysis
#13
Zhe Ling, Sheng Chen, Xueming Zhang, Keiji Takabe, Feng Xu
Ionic liquid (IL) is one of the pretreatment processes gaining considerable interests to remove the native recalcitrance of lignocellulose. But the cellulose crystalline transformation during the pretreatment and their correlations with enzymatic digestibility have not been fully elucidated. Microcrystalline cellulose (Avicel) and holocellulose, which have differential sources and original crystallinity, were respectively pretreated with 1-butyl-3-methylimidazolium chloride ([C4min]Cl). Cellulose crystalline variations as well as chemical and morphological changes were determined...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28852423/single-cell-oil-production-by-trichosporon-cutaneum-from-steam-exploded-corn-stover-and-its-upgradation-for-production-of-long-chain-%C3%AE-%C3%AF-dicarboxylic-acids
#14
Chen Zhao, Hao Fang, Shaolin Chen
BACKGROUND: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based bioprocess to more valuable ones is becoming a more and more important issue. RESULTS: Differently sourced cellulases were compared in the enzymatic hydrolysis of the steam-exploded corn stover (SECS) and the cellulase from the mixed culture of Trichoderma reesei and Aspergillus niger was found to have the highest enzymatic hydrolysis yield 86...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28822953/xylanase-from-acinetobacter-pittii-mask-25-and-developed-magnetic-cross-linked-xylanase-aggregate-produce-predominantly-xylopentose-and-xylohexose-from-agro-biomass
#15
Anjali Purohit, Shushil Kumar Rai, Manisha Chownk, Rajender Singh Sangwan, Sudesh Kumar Yadav
Most of the chemical and biochemical processes used for the de-polymerization of structural polymers of lignocellulosic biomass are environment unfriendly and costly. Here an efficient process based on xylanase, produced by Acinetobacter pittii MASK25 (MTCC 25132), hydrolysis of only physically treated rice straw and corn cob has been developed for the production of xylooligosaccharides. Bacterial strain isolated from soil was found to produce maximum xylanase at 30°C and pH 7. While the optimum temperature and pH of xylanase were characterized as 40°C and 5...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28803111/laccase-enzyme-detoxifies-hydrolysates-and-improves-biogas-production-from-hemp-straw-and-miscanthus
#16
Michel Schroyen, Stijn W H Van Hulle, Sander Holemans, Han Vervaeren, Katleen Raes
The impact of various phenolic compounds, vanillic acid, ferulic acid, p-coumaric acid and 4-hydroxybenzoic acid on anaerobic digestion of lignocellulosic biomass (hemp straw and miscanthus) was studied. Such phenolic compounds have been known to inhibit biogas production during anaerobic digestion. The different phenolic compounds were added in various concentrations: 0, 100, 500, 1000 and 2000mg/L. A difference in inhibition of biomethane production between the phenolic compounds was noted. Hydrolysis rate, during anaerobic digestion of miscanthus was inhibited up to 50% by vanillic acid, while vanillic acid had no influence on the initial rate of biogas production during the anaerobic digestion of hemp straw...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28797630/enzymatic-in-situ-saccharification-of-sugarcane-bagasse-pretreated-with-low-loading-of-alkalic-salts-na2so3-na3po4-by-autoclaving
#17
Chun-Xia Jiang, Yu-Cai He, Gang-Gang Chong, Jun-Hua Di, Ya-Jie Tang, Cui-Luan Ma
Sugarcane bagasse (SCB) is an abundant, renewable and inexpensive agricultural byproduct for the production of biofuel and other biobased products. To effectively saccharify SCB with cellulases, combination with dilute alkali salts Na2SO3/Na3PO4 (0.4% Na3PO4, 0.03% Na2SO3) at 7.5% sulfidity and hot water (DASHW) in "one-pot" pretreatment media by autoclaving at 110°C for 40min was attempted to pretreat SCB in this study. Furthermore, FT-IR, XRD and SEM were employed to characterize the changes in the cellulose structural characteristics (porosity, morphology, and crystallinity) of the pretreated Na2SO3/Na3PO4-SCB solid residue, which indicated that combination pretreatment could effectively remove lignin and hemicellulose for enhancing enzymatic saccharification...
August 8, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28777990/additives-enhancing-enzymatic-hydrolysis-of-lignocellulosic-biomass
#18
Javier Rocha-Martín, Claudio Martinez-Bernal, Yolanda Pérez-Cobas, Francisco Manuel Reyes-Sosa, Bruno Díez García
Linked to the development of cellulolytic enzyme cocktails from Myceliophthora thermophila, we studied the effect of different additives on the enzymatic hydrolysis yield. The hydrolysis of pretreated corn stover (PCS), sugar cane straw (PSCS) and microcrystalline cellulose (Avicel) was performed under industrial conditions using high solid loadings, limited mixing, and low enzyme dosages. The addition of polyethylene glycol (PEG4000) allowed to increase the glucose yields by 10%, 7.5%, and 32%, respectively in the three materials...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28764128/using-temperature-responsive-zwitterionic-surfactant-to-enhance-the-enzymatic-hydrolysis-of-lignocelluloses-and-recover-cellulase-by-cooling
#19
Cheng Cai, Yuxia Pang, Xuejuan Zhan, Meijun Zeng, Hongming Lou, Yong Qian, Dongjie Yang, Xueqing Qiu
Some zwitterionic surfactants exhibit upper critical solution temperature (UCST) in aqueous solutions. For the zwitterionic surfactant solution mixed with cellulase, when its temperature is below UCST, the cellulase can be recovered by coprecipitation with zwitterionic surfactant. In this work, 3-(Hexadecyldimethylammonio) propanesulfonate (SB3-16) was selected to enhance the enzymatic hydrolysis of lignocelluloses and recover the cellulase. After adding 2mmol/L of SB3-16, the enzymatic digestibility of eucalyptus pretreated by dilute acid (Eu-DA) and by sulfite (Eu-SPORL) increased from 27...
July 13, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28726354/high-performance-of-agaricus-blazei-fungus-for-the-biological-pre-treatment-of-elephant-grass
#20
Thais Dal Picolli, Kaliane Regalin Aver, Roselei Claudete Fontana, Marli Camassola
Biological pre-treatment seems to be promising being an eco-friendly process, with no inhibitor generated during the process. The potential for elephant grass pre-treatment with white degradation fungi Pleurotus ostreatus, Agaricus blazei, Lentinula edodes, Pleurotus citrinopileatus and Pleurotus djamor, in isolated or mixed cultures of these strains, was evaluated. The highest activities of enzymes involved in the degradation of lignocellulosic biomass (laccases, endoglucanases, xylanases and β-glucosidases) were observed for A...
July 20, 2017: Biotechnology Progress
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