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Lignocellulosic enzymatic hydrolysis

Thais Dal Picolli, Kaliane Regalin Aver, Roselei Claudete Fontana, Marli Camassola
Biological pre-treatment seems to be promising being an eco-friendly process, with no inhibitor generated during the process. The potential for elephant grass pre-treatment with white degradation fungi Pleurotus ostreatus, Agaricus blazei, Lentinula edodes, Pleurotus citrinopileatus and Pleurotus djamor, in isolated or mixed cultures of these strains, was evaluated. The highest activities of enzymes involved in the degradation of lignocellulosic biomass (laccases, endoglucanases, xylanases and β-glucosidases) were observed for A...
July 20, 2017: Biotechnology Progress
Saksit Imman, Navadol Laosiripojana, Verawat Champreda
Liquid hot water (LHW) pretreatment is an efficient chemical-free strategy for enhancing enzymatic digestibility of lignocellulosic biomass for conversion to fuels and chemicals in biorefinery. In this study, effects of LHW on removals of hemicelluloses and lignin from corncobs were studied under varying reaction conditions. LHW pretreatment at 160 °C for 10 min promoted the highest levels of hemicellulose solubilization into the liquid phase, resulting into the maximized pentose yield of 58.8% in the liquid and more than 60% removal of lignin from the solid, with 73...
July 19, 2017: Applied Biochemistry and Biotechnology
Xiaolong Han, Guodong Liu, Wenxia Song, Yinbo Qu
The production of sodium gluconate by enzymatic catalysis of delignified corn cob residue (DCCR) hydrolysate was studied. Penicillium oxalicum I1-13 was used for the production of cellulase with high β-glucosidase activity. A fed-batch saccharification process was developed to obtain high yields of glucose. At the end of hydrolysis, the concentration of glucose reached 145.80g/L. Glucose oxidase and catalase were co-immobilized to catalyze DCCR hydrolysate to produce sodium gluconate. Under the optimum conditions, 166...
June 23, 2017: Bioresource Technology
Lucía Martín Pérez, Laura Benítez Casanova, Antonio Javier Moreno Pérez, Dolores Pérez Gómez, Sandra Gavaldá Martín, Laura Ledesma-García, Noelia Valbuena, Bruno Díez García, Francisco Manuel Reyes-Sosa
Thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years. Although the needs for the reduction of the energy intensity and chemical consumption in the pretreatment step are well known, the reduction of the severity of the process strongly affects the enzymatic hydrolysis yield. To explore the formulation requirements of the well known cellulolytic cocktail from Myceliophthora thermophila on mild pretreated raw materials, this cocktail was tested on steam exploded corn stover without acid impregnation...
July 15, 2017: Biotechnology and Bioengineering
Piotr Chylenski, Dejan M Petrović, Gerdt Müller, Marie Dahlström, Oskar Bengtsson, Martin Lersch, Matti Siika-Aho, Svein Jarle Horn, Vincent G H Eijsink
BACKGROUND: Recent advances in the development of enzyme cocktails for degradation of lignocellulosic biomass, especially the discovery of lytic polysaccharide monooxygenases (LPMOs), have opened new perspectives for process design and optimization. Softwood biomass is an abundant resource in many parts of the world, including Scandinavia, but efficient pretreatment and subsequent enzymatic hydrolysis of softwoods are challenging. Sulfite pulping-based pretreatments, such as in the BALI™ process, yield substrates that are relatively easy to degrade...
2017: Biotechnology for Biofuels
Zhonghai Li, Guodong Liu, Yinbo Qu
Filamentous fungi are considered as the most efficient producers expressing lignocellulose-degrading enzymes. Penicillium oxalicum strains possess extraordinary fungal lignocellulolytic enzyme systems and can efficiently utilize plant biomass. In recent years, the regulatory aspects of production of hydrolytic enzymes by P. oxalicum have been well established. This review aims to discuss the recent developments for the production of lignocellulolytic enzymes by P. oxalicum. The main cellulolytic transcription factors mediating the complex transcriptional-regulatory network are highlighted...
June 23, 2017: Bioresource Technology
Rajeev Ravindran, Chaitanya Sarangapani, Swarna Jaiswal, P J Cullen, Amit K Jaiswal
In this study, a novel pretreatment for spent coffee waste (SCW) has been proposed which combines two techniques viz. atmospheric air plasma and FeCl3 to create a superior pretreatment that involves Fenton chemistry. The pretreatment was optimised employing Taguchi Design of Experiments, and five parameters were taken into consideration viz. biomass loading, FeCl3 concentration, H2SO4 concentration, plasma discharge voltage and treatment time. The composition analysis of the pretreated SCW revealed substantial amounts of lignin removal, with a maximum for process conditions of 70kV for 2min in an acidic environment containing 1% H2SO4...
June 26, 2017: Bioresource Technology
Siti Sabrina Mohd Sukri, A M Mimi Sakinah
The present study explores the utilisation of a new raw material from lignocellulose biomass, Meranti wood sawdust (MWS) for high commercial value xylooligosaccharides (XOS) production using immobilised xylanase. The xylanase was immobilised by a combination of entrapment and covalent binding techniques. The hemicellulosic xylan from MWS was extracted using a standard chlorite delignification method. The production of total and derivatives of XOS from the degradation of the hemicellulosic xylan of MWS were compared to the production from the commercial xylan from Beechwood...
July 4, 2017: Applied Biochemistry and Biotechnology
José Lucas de Almeida Antunes Ferraz, Lucas Oliveira Souza, Glêydison Amarante Soares, Janclei Pereira Coutinho, Julieta Rangel de Oliveira, Elizama Aguiar-Oliveira, Marcelo Franco
The aim of this work was to enzymatic saccharification of food waste was performed by crude enzymatic cellulolytic extract produced by P. roqueforti cultivated in yellow mombin residue. The best yield of reducing sugars (259.45mgg(-1)) was achieved with sugarcane bagasse after 4h; the hydrolysis of corn cob, rice husk and peanut hull resulted in yields around 128-180mgg(-1). The addition of 10mmolL(-1) of Mn(2+) potentiated the saccharification of sugarcane bagasse, in about 86%. The temperature and substrate (sugarcane bagasse) concentration parameters were optimized using a Doehlert Design and, a maximum sugar yield of 662...
June 12, 2017: Bioresource Technology
Stefanie Arnold, Karin Moss, Marius Henkel, Rudolf Hausmann
Lignocellulosic biomass is an important feedstock for a potential future bio-based economy. Owing to its compact structure, suitable decomposition technologies will be necessary to make it accessible for biotechnological conversion. While chemical and enzymatic hydrolysis are currently established methods, a promising alternative is provided by fast pyrolysis. The main resulting product thereof, referred to as pyrolysis oil, is an energy-rich and easily transportable liquid. Many of the identified constituents of pyrolysis oil, however, have previously been reported to display adverse effects on microbial growth...
June 27, 2017: Trends in Biotechnology
Marek Vochozka, Vojtěch Stehel, Anna Maroušková
Notable progress has been achieved in the past two decades regarding production of different enzymatic mixtures for hydrolysis of the lignocellulose matrix. Nevertheless, the hydrolysing mixtures remain slow and require tempering, which results in high-energy demands and bad financial results. Use of acids or alkali at a very high temperature and pressure accelerates the process more than ten times wherein the energy requirements are approximately equal. However, these elevated reaction conditions might cause the breakdown of complex lignin formula into substances that have the potential to inhibit subsequent fermentation processes...
July 2017: 3 Biotech
Dong Tian, Richard P Chandra, Jin-Suk Lee, Canhui Lu, Jack N Saddler
BACKGROUND: Current single-stage delignification-pretreatment technologies to overcome lignocellulosic biomass recalcitrance are usually achieved at the expense of compromising the recovery of the polysaccharide components, particularly the hemicellulose fraction. One way to enhance overall sugar recovery is to tailor an efficient two-stage pretreatment that can pre-extract the more labile hemicellulose component before subjecting the cellulose-rich residual material to a second-stage delignification process...
2017: Biotechnology for Biofuels
Francisco Manuel Reyes-Sosa, Macarena López Morales, Ana Isabel Platero Gómez, Noelia Valbuena Crespo, Laura Sánchez Zamorano, Javier Rocha-Martín, Fernando P Molina-Heredia, Bruno Díez García
BACKGROUND: Modern biorefineries require enzymatic cocktails of improved efficiency to generate fermentable sugars from lignocellulosic biomass. Cellulolytic fungi, among other microorganisms, have demonstrated the highest potential in terms of enzymatic productivity, complexity and efficiency. On the other hand, under cellulolytic-inducing conditions, they often produce a considerable diversity of carbohydrate-active enzymes which allow them to adapt to changing environmental conditions...
2017: Biotechnology for Biofuels
Anderson Albino Gomes, Elisa Pazinatto Telli, Luiz Claudio Miletti, Everton Skoronski, Marcos Gomes Ghislandi, Gustavo Felippe da Silva, Maria de Lourdes Borba Magalhães
Optimization of cellulose enzymatic hydrolysis is crucial for cost-effective bioethanol production from lignocellulosic biomass. Enzyme immobilization in solid support allows enzyme recycling for reuse, lowering hydrolysis costs. Graphene is a nanomaterial isolated in 2004, which possesses exceptional properties for biomolecule immobilization. This study evaluates the potential for β-glucosidase recycling by immobilization on graphene nanosheets. Data reported here demonstrated that graphene-immobilized β-glucosidase can be recycled for at least eight cycles...
June 21, 2017: Biotechnology and Applied Biochemistry
Thiago Rodrigues Dutra, Valéria Monteze Guimarães, Ednilson Mascarenhas Varela, Lílian da Silva Fialho, Adriane Maria Ferreira Milagres, Daniel Luciano Falkoski, José Cola Zanuncio, Sebastião Tavares de Rezende
Low cost and high efficiency cellulolytic cocktails can consolidate lignocellulosic ethanol technologies. Sugarcane bagasse (SCB) is a low cost agro-industrial residue, and its use as a carbon source can reduce the costs of fungi cultivation for enzyme production. Chrysoporthe cubensis grown under solid state fermentation (SSF) with wheat bran has potential to produce efficient enzymatic extracts for SCB saccharification. This fungus was grown under submersed fermentation (SmF) and SSF with in natura SCB, pretreated with acid or alkali and with others carbon sources...
June 20, 2017: Scientific Reports
Hiroshi Teramura, Kengo Sasaki, Hideo Kawaguchi, Fumio Matsuda, Jun Kikuchi, Tomokazu Shirai, Takashi Sazuka, Masanori Yamasaki, Shigeo Takumi, Chiaki Ogino, Akihiko Kondo
Bio-refinery processes require use of the most suitable lignocellulosic biomass for enzymatic saccharification and microbial fermentation. Glucose yield from biomass solid fractions obtained after dilute sulfuric acid (1%) pretreatment (at 180 °C) was investigated using 14, 8, and 16 varieties of rice, wheat, and sorghum, respectively. Biomass solid fractions of each crop showed similar cellulose content. However, glucose yield after enzymatic hydrolysis (cellulase loading at 6.6 filter paper unit/g-biomass) was different among the varieties of each crop, indicating genotypic differences for rice, wheat, and sorghum...
August 2017: Bioscience, Biotechnology, and Biochemistry
Di Huang, Jia Liu, Yanfei Qi, Kexin Yang, Yingying Xu, Lu Feng
Lignocellulosic biomass from various types of wood has become a renewable resource for production of biofuels and biobased chemicals. Because xylan is the major component of wood hemicelluloses, highly efficient enzymes to enhance xylan hydrolysis can improve the use of lignocellulosic biomass. In this study, a xylanolytic gene cluster was identified from the crude oil-degrading thermophilic strain Geobacillus thermodenitrificans NG80-2. The enzymes involved in xylan hydrolysis, which include two xylanases (XynA1, XynA2), three β-xylosidases (XynB1, XynB2, XynB3), and one α-L-arabinofuranosidase (AbfA), have many unique features, such as high pH tolerance, high thermostability, and a broad substrate range...
June 14, 2017: Applied Microbiology and Biotechnology
Rameshwar Tiwari, Lata Nain, Nikolaos E Labrou, Pratyoosh Shukla
Second generation biofuel production has been appeared as a sustainable and alternative energy option. The ultimate aim is the development of an industrially feasible and economic conversion process of lignocellulosic biomass into biofuel molecules. Since, cellulose is the most abundant biopolymer and also represented as the photosynthetically fixed form of carbon, the efficient hydrolysis of cellulose is the most important step towards the development of a sustainable biofuel production process. The enzymatic hydrolysis of cellulose by suites of hydrolytic enzymes underlines the importance of cellulase enzyme system in whole hydrolysis process...
June 13, 2017: Critical Reviews in Microbiology
Phisit Seesuriyachan, Arthitaya Kawee-Ai, Thanongsak Chaiyaso
In this study, the pressures at 50-500MPa were evaluated at different time to pretreat and further enzyme hydrolysis. The ultra-high pressure (UHP) pretreatment at 100MPa for 10min led to improved accessibility of enzyme for conversion of xylan to xylooligosaccharide (XOS). The maximum XOS yield of 35.6mg/g substrate was achieved and firstly reported at 10% (w/v) of substrate, 100U of endo-xylanase/g corncobs and incubation time of 18h. The enzymatic hydrolysis efficiency was increased by 180.3% and released a high amount of xylobiose...
October 2017: Bioresource Technology
Linfeng Kou, Yanliang Song, Xu Zhang, Tianwei Tan
In order to utilize energy grasses as substrates for production of biofuels and expand the diversity of lignocellulosic feedstocks, this work developed an evaluation system considering 16 kinds of parameters related to the contents of three main compositions (cellulose, hemicellulose and lignin), inhibitors generated from the pretreatment, the extent of enzymatic hydrolysis and microbial fermentation. Giant reed, corn stalks, switch grass, pennisetum and silvergrass were investigated. Comprehensive utilization indexes of giant reed (55...
October 2017: Bioresource Technology
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