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Laure Gossec, Frédéric Guyard, Didier Leroy, Thomas Lafargue, Michel Seiler, Charlotte Jacquemin, Anna Molto, Jérémie Sellam, Violaine Foltz, Frédérique Gandjbakhch, Christophe Hudry, Stéphane Mitrovic, Bruno Fautrel, Hervé Servy
BACKGROUND: Flares in rheumatoid arthritis (RA) and axial spondyloarthritis (axSpA) may influence physical activity. The objective was to assess longitudinally the association between patient-reported flares and activity-tracker-provided steps per minute, using machine-learning. METHODS: This prospective observational study (ActConnect) included patients with definite RA or axSpA. During 3 months, physical activity was assessed continuously by number of steps/minute, using a consumer grade activity tracker, and flares were self-assessed weekly...
September 22, 2018: Arthritis Care & Research
Johanna Balslev Andersen, Julie Yoon Moberg, Janni Niclasen, Bjarne Laursen, Melinda Magyari
BACKGROUND: Multiple sclerosis is associated with an increased risk of developing physical, cognitive, and mental health problems. Current studies have demonstrated variating outcomes of parental multiple sclerosis mental health problems and their children's mental health development. OBJECTIVE: The purpose of this study was to investigate whether maternal multiple sclerosis is associated with the mental health status of their child. METHODS: Data from the Danish National Birth Cohort (DNBC) were merged with information from the Danish Multiple Sclerosis Registry...
September 21, 2018: Brain and Behavior
Christina Lindén, Anders Heijl, Gauti Jóhannesson, Johan Aspberg, Sabina Andersson Geimer, Boel Bengtsson
PURPOSE: To study newly diagnosed glaucoma patients given mono- or multi-therapy regarding differences in initial intraocular pressure (IOP) reduction, target IOP levels reached and influence of untreated baseline IOP on IOP reduction. METHODS: Patients newly diagnosed with manifest primary open-angle glaucoma and included in the Glaucoma Intensive Treatment Study (GITS) were randomized to immediate intensive treatment with any of three different IOP-lowering substances supplied in two bottles plus 360° laser trabeculoplasty or to conventional stepwise treatment starting with a single-drug...
September 21, 2018: Acta Ophthalmologica
Ahmed M Abu El-Asrar, Nele Berghmans, Saleh A Al-Obeidan, Priscilla W Gikandi, Ghislain Opdenakker, Jo Van Damme, Sofie Struyf
PURPOSE: To determine the concentrations of the CC chemokines CCL2, CCL7, CCL8, CCL11, CCL13, CCL20, CCL24 and CCL26 in aqueous humour (AH) samples from patients with specific uveitic entities. METHODS: Aqueous humour samples from patients with active uveitis associated with Behçet's disease (BD) (n = 13), sarcoidosis (n = 8), HLA-B27-related inflammation (n = 12), Vogt-Koyanagi-Harada (VKH) disease (n = 12) and control patients (n = 9) were assayed with the use of a multiplex assay...
September 21, 2018: Acta Ophthalmologica
Jingjing Da, Yan Ran, Mingjing Pi, Jing Wu, Rong Dong, Qian Li, Qian Zhang, Xiangyan Zhang, Yan Zha
Medical education paradigm has been questioned for the requirements of improving the quality and quantity of medical students. This study was to explore the efficiency of integrated-based learning (IBL) used mini-clinical evaluation exercise (mini-CEX) during physical diagnostics course. One hundred and eleven volunteered students were randomly divided into three groups: lecture-based learning (LBL), case-based learning (CBL), and IBL. Nephrotic syndrome was the teaching content. In the IBL group, students were provided the guideline and additional interpretation from the instructor about the basic knowledge related to disease as vertical integration curriculum...
September 22, 2018: Biochemistry and Molecular Biology Education
En Yi Joanne Chin, Vijay R Baral, Imelda L Ereno, John C Allen, Kelly Low, Cheo Lian Yeo
AIM: We hypothesise that clinically well late-preterm infants (LPI) (34+0 -36+6 weeks) are neurologically more immature than their term counterparts, and this immaturity persists even when these infants reach term-corrected age (TCA). The primary aim of our study was to characterise and contrast the neurodevelopmental profile of well LPI with full-term infants (FTI) (39+0 -41+6 weeks) using the Hammersmith Neonatal Neurological Examination (HNNE). Our secondary aim was to obtain local reference ranges for the 34 items in the HNNE in an Asian-dominant population...
September 21, 2018: Journal of Paediatrics and Child Health
Beata Kmiec, Rui M M Branca, Oliver Berkowitz, Lu Li, Yan Wang, Monika W Murcha, James Whelan, Janne Lehtiö, Elzbieta Glaser, Pedro F Teixeira
The stepwise degradation of peptides to amino acids in plant mitochondria and chloroplasts is catalyzed by a network of oligopeptidases (presequence protease PreP, organellar oligopeptidase OOP) and aminopeptidases. In the present report, we show that the lack of oligopeptidase activity in Arabidopsis thaliana results in the accumulation of endogenous free peptides, mostly of chloroplastic origin (targeting peptides and degradation products). Using mRNA sequencing and deep coverage proteomics, allowing for the identification of 17000 transcripts and 11000 proteins, respectively, we uncover a peptide-stress response occurring in plants lacking PreP and OOP oligopeptidase activity...
September 22, 2018: Plant Journal: for Cell and Molecular Biology
Tsuyoshi Ichikawa, Masanori Yokoba, Masahiko Kimura, Manaka Shibuya, Paul A Easton, Masato Katagiri
NEW FINDINGS: What is the central question of this study? Maximal sniff nasal inspiratory and reverse sniff nasal expiratory pressures are measured as inspiratory and expiratory muscle strength, respectively. Is the genioglossus muscle activated during short maximal inspiratory and expiratory efforts through the nose? What is the main finding and its importance? Genioglossus muscle activity occurred with inspiratory muscle activity during a maximal sniff, and with expiratory muscle activity during a maximal reverse sniff...
September 22, 2018: Experimental Physiology
A Scope, S W Dusza, G Pellacani, M Gill, S Gonzalez, M A Marchetti, H S Rabinovitz, A A Marghoob, C Alessi-Fox, A C Halpern
BACKGROUND: Diagnostic accuracy of reflectance confocal microscopy (RCM) as a stand-alone diagnostic tool for suspect skin lesions has not been extensively studied. OBJECTIVE: Primary aim was to measure experts' accuracy in RCM-based management decisions. Secondary aim was to identify melanoma-specific RCM features. METHODS: The study enrolled patients ≥18 years that underwent biopsy of skin lesions clinically suspected to be melanoma. One hundred lesions imaged by RCM were randomly-selected from 439 lesions prospectively collected at four pigmented lesion clinics...
September 22, 2018: Journal of the European Academy of Dermatology and Venereology: JEADV
Chunyan Liu, Junyi Tang, Chang Li, Guangbo Pu, Dongxia Yang, Xiaotian Chang
An increasing amount of evidence indicates that peptidylarginine deiminase isoform 4 (PADI4) plays an important role in tumorigenesis. However, the effects of PADI4 on tumor-bearing mice are unknown, and no studies have investigated this tumorigenic pathway in an animal model. In the present study, ECA109 cells originating from esophageal squamous cell carcinoma (ESCC) were transfected with PADI4-expressing lentivirus and were injected into BALB/c nude mice. Tumor size and weight were significantly increased in the mouse tumors established with PADI4-overexpressing ECA109 cells...
September 22, 2018: Molecular Carcinogenesis
Petter Brattström, Chiara Russo, David Ley, Matteo Bruschettini
AIM: Though caffeine is a consolidated treatment in preterm infants, the efficacy and safety of a higher dose have not been systematically appraised. METHODS: A systematic review was conducted to compare high (loading dose >20 mg/kg and maintenance >10 mg/kg/day) versus low dose of caffeine. MEDLINE, EMBASE, Central, and conference proceedings for randomized controlled trials (RCTs) and quasi-RCTs were searched. Two authors independently screened the records, extracted the data and assessed the risk of bias...
September 22, 2018: Acta Paediatrica
Keiichi Akahoshi, Daisuke Ban, Ryo Kuboki, Atsushi Oba, Hiroaki Ono, Yusuke Mitsunori, Atsushi Kudo, Shinji Tanaka, Minoru Tanabe
BACKGROUND AND AIM: To improve the prognosis of cholangiocarcinoma, we investigated potential biomarkers that may enable the selection of patients for whom postoperative adjuvant chemotherapy is likely effective. METHODS: The cohort of this retrospective study included 170 surgically-resected cholangiocarcinoma patients, 26 with gemcitabine adjuvant chemotherapy (GEM group), 36 with S-1 adjuvant chemotherapy (S-1 group), and 103 receiving no adjuvant chemotherapy (NC group)...
September 22, 2018: Journal of Gastroenterology and Hepatology
Mustafa Kara, Seda Sabah Ozcan, Turhan Aran, Ozlem Kara, Neziha Yilmaz
OBJECTIVE: The aim of this study was to evaluate endometrial receptivity by measuring HOXA-10, HOXA-11, and LIF gene expressions in women with polycystic ovary syndrome. METHODS: A total of 48 women were included in this clinical study. Thepatients were allocated to two groups: study group consisted of 28 patients with myoma uteri and control group consisted of 20 patients without myoma uteri. Endometrial sampling was performed during the proliferative phase. The biopsies obtained from the patients with myoma uteri were taken from the place where the fibroids were localized...
September 22, 2018: Journal of Cellular Biochemistry
Yani Zhang, Yingjie Wang, Bi Yulin, Beibei Tang, Man Wang, Chen Zhang, Wenhui Zhang, Jing Jin, Tingting Li, Ruifeng Zhao, Xinjian Yu, Qisheng Zuo, Bichun Li
Myostatin (MSTN) is an important gene involved in the regulation of embryonic muscle cells and adult muscle development; it has a good application prospect in transgenic animal production by improving the yield of muscle. The purpose of this study is to construct MSTN gene knockout vector using clustered regularly interspaced short palindromic repeats ( CRISPR)/CRISPR-associated protein 9 ( Cas9). The knockout efficiency was evaluated in sheep ear fibroblasts (SEFs) by cleavage activity of transcription of guide RNA ( gRNA), luciferase-single-strand annealing assay, T7 endonuclease I assay (T7E1), and TA clone sequence (10/38); and above all, detection showed that the cleavage activity of CRISPR/Cas9-mediated MSTN reached 29%...
September 22, 2018: Journal of Cellular Biochemistry
Jiao Li, Yi Chen, Lianli Shen, Yueyi Deng
The objective of this paper was to explore the role and molecular mechanism of miR-193a in membranous nephropathy (MN). Experimental rats and podocytes were randomly divided into four groups: control, MN, miR-NC, and miR-193a inhibitor groups. The relative mRNA level of miR-193a was determined. The mRNA level and protein expression of PODXL, NPHS1, and Notch1 were determined by real-time polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. The mRNA level and protein expression of WT1 in podocytes were also determined by RT-PCR and Western blot analysis...
September 22, 2018: Journal of Cellular Biochemistry
Rooma Adalat, Faiza Saleem, Aftab Bashir, Munir Ahmad, Soumble Zulfiqar, Abdul Rauf Shakoori
Cry2Ac11, a 65 kDa insecticidal protein produced by Bacillus thuringiensis, shows toxicity against dipteran and lepidopteran larvae. It is encoded by cry2Ac11 gene ( orf3), which is part of an operon comprising orf1, orf2, and orf3. Orf2, a helper protein, helps in proper folding and prevents aberrant aggregation of newly produced molecules. In this study, we have elucidated the effect of different mutations in translation initiation region (TIR), particularly the ribosomal binding site and the start codon (RBS-ATG) on cry2Ac11 gene expression without helper protein...
September 22, 2018: Journal of Cellular Biochemistry
Jiajia Wang, Zongliang Lu, Changpeng Wu, Yanwu Li, Ya Kong, Rui Zhou, Kun Shi, Jing Guo, Na Li, Jie Liu, Wei Song, He Wang, Mingxing Zhu, Hongxia Xu
BACKGROUND: Voltage-gated sodium channels (VGSCs) are involved in several cellular processes related to cancer cell growth and metastasis, including adhesion, proliferation, apoptosis, migration, and invasion. We here in investigated the effects of S0154 and S0161, two novel synthetic sodium channel blockers (SCBs), on human prostate cancer cells (PC3, DU145, and LnCaP) and a prostate cancer xenograft model. METHODS: The MTT assay was used to assess the anticancer effects of SCBs in PC3, DU145, and LnCaP cells...
September 21, 2018: Prostate
Kathleen A Kraska, Joshua Jackson
OBJECTIVE: To determine the effect of the method used to attach OrthoFiber #5 to a 1/8-in Securos toggle rod on the mechanical properties of the resulting construct. STUDY DESIGN: Prospective bench top mechanical analysis. SAMPLE POPULATION: One hundred twenty-eight OrthoFiber #5-toggle rod suture constructs. METHODS: Toggle-suture constructs (groups A-H) were prepared by using 8 methods to attach OrthoFiber #5 to a 1/8-in toggle rod (n = 16 constructs/group)...
September 21, 2018: Veterinary Surgery: VS
Jing Cui, Cyril Abadie, Adam Carroll, Emmanuelle Lamade, Guillaume Tcherkez
K deficiency and waterlogging are common stresses that can occur simultaneously and impact on crop development and yield. They are both known to affect catabolism, with rather opposite effects: inhibition of glycolysis and higher glycolytic fermentative flux, respectively. But surprisingly, the effect of their combination on plant metabolism has never been examined precisely. Here, we applied a combined treatment (K availability, waterlogging) to sunflower (Helianthus annuus L.) plants under controlled greenhouse conditions, and performed elemental quantitation, metabolomics and isotope analyses at different sampling times...
September 22, 2018: Plant, Cell & Environment
Sonja Axmann, Karin Hummel, Katharina Nöbauer, Ebrahim Razzazi-Fazeli, Karin Zitterl-Eglseer
Devil's claw is used for the treatment of inflammatory symptoms and degenerative disorders in horses since many years, but without the substantive pharmacokinetic data. The pharmacokinetic parameters of harpagoside, the main active constituent of Harpagophytum procumbens DC ex Meisn., were evaluated in equine plasma after administration of Harpagophytum extract FB 8858 in an open, single-dose, two-treatment, two-period, randomized cross-over design. Six horses received a single dose of Harpagophytum extract, corresponding to 5 mg/kg BM harpagoside, and after 7 days washout period, 10 mg/kg BM harpagoside via nasogastric tube...
September 22, 2018: Journal of Veterinary Pharmacology and Therapeutics
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