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https://www.readbyqxmd.com/read/28454319/construction-and-expression-of-a-lentivirus-expression-vector-carrying-the-vegf165-egfp-fusion-gene-in-breast-cancer-mcf-7-cells
#1
Minna Luo, Huan Huang, Lei Hou, Shan Shao, Shangke Huang, Xinhan Zhao
Vascular endothelial growth factor (VEGF)165 is one of the most abundant and potent angiogenic factors in both physiological and pathological conditions. However, the function and mechanism of VEGF165 in tumors and their environment remain to be elucidated. In the present study, a lentivirus vector (LV) that contained the VEGF165-enhanced green fluorescent protein (EGFP) fusion gene was constructed and transfected into the human breast cancer cell line MCF-7. Following transfection, the expression of VEGF165 in MCF-7 cells was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454193/-mental-stress-of-professionals-in-residential-care-for-children-and-adolescents
#2
Thea Rau, Jeannine Ohlert, Corinna Seidler, Jörg M Fegert, Marc Allroggen
An increasingly important issue is the mental strain of professionals in residential care for children and adolescents. However, only few studies investigate mental strain and overload of professionals in an educational context in general and even less in youth welfare institutions. The goal of this study was to examine mental strain experiences of professionals with the "Perceived Stress Questionnaire" (PSQ) in youth welfare institutions, as well as to investigate the relationship between age, duration of employment in this work environment and gender of participants...
April 28, 2017: Psychotherapie, Psychosomatik, Medizinische Psychologie
https://www.readbyqxmd.com/read/28454169/speckle-based-hyperspectral-imaging-combining-multiple-scattering-and-compressive-sensing-in-nanowire-mats
#3
Rebecca French, Sylvain Gigan, Otto L Muskens
Encoding of spectral information onto monochrome imaging cameras is of interest for wavelength multiplexing and hyperspectral imaging applications. Here, the complex spatiospectral response of a disordered material is used to demonstrate retrieval of a number of discrete wavelengths over a wide spectral range. Strong, diffuse light scattering in a semiconductor nanowire mat is used to achieve a highly compact spectrometer of micrometer thickness, transforming different wavelengths into distinct speckle patterns with nanometer sensitivity...
May 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28454154/photoacoustic-imaging-of-dental-implants-in-a-porcine-jawbone-ex-vivo
#4
Donghyun Lee, Sungjo Park, Woo-Chang Noh, Jae-Seung Im, Chulhong Kim
Currently, x-ray-based imaging is used before and after the dental implant treatment, but the ionizing radiation is potentially harmful to patients and operators. Here, we demonstrate ex vivo photoacoustic imaging of a dental implant embedded in a porcine jawbone. By layering biological tissue over the jawbone to mimic a clinical environment, we demonstrate 10 mm deep imaging. Our results show that photoacoustic imaging can provide jawbone anatomical information, the location of an embedded implant fixture, and the thickness of the soft tissue above the jawbone...
May 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28454063/interaction-of-amino-acid-functionalized-silver-nanoparticles-and-candida-albicans-polymorphs-a-deep-uv-fluorescence-imaging-study
#5
Radovan Dojčilović, Jelena D Pajović, Dušan K Božanić, Una Bogdanović, Vesna V Vodnik, Suzana Dimitrijević-Branković, Miona G Miljković, Slavka Kaščaková, Matthieu Réfrégiers, Vladimir Djoković
The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores...
April 23, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28454047/use-of-light-emitting-diodes-leds-for-enhanced-lipid-production-in-micro-algae-based-biofuels
#6
Alifha Severes, Shashank Hegde, L D'Souza, Smitha Hegde
Microalgae are an alternative source for renewable energy to overcome the energy crises caused by exhaustion of fuel reserves. Algal biofuel technology demands a cost effective strategy for net profitable productivity. Inconsistent illumination intensities hinder microalgal growth. The light-utilizing efficiency of the cells is critical. Light scarcity leads to low production and high intensities cause photo-inhibition. We report effective usage of LEDs of different band wavelengths on the growth of microalgae in a closed, controlled environment to generate biomass and lipid yields...
April 20, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28454041/long-term-intake-of-a-high-protein-diet-increases-liver-triacylglycerol-deposition-pathways-and-hepatic-signs-of-injury-in-rats
#7
Rubén Díaz-Rúa, Jaap Keijer, Andreu Palou, Evert M van Schothorst, Paula Oliver
Intake of high-protein (HP) diets has increased over the last years, mainly due to their popularity for body weight control. Liver is the main organ handling ingested macronutrients and it is associated with the beginning of different pathologies. We aimed to deepen our knowledge on molecular pathways affected by long-term intake of an HP diet. We performed a transcriptome analysis on liver of rats chronically fed with a casein-rich HP diet and analyzed molecular parameters related to liver injury. Chronic increase in the dietary protein/carbohydrate ratio up-regulated processes related with amino acid uptake/metabolism and lipid synthesis, promoting a molecular environment indicative of hepatic triacylglycerol (TG) deposition...
April 19, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28454040/the-role-of-dissimilatory-arsenate-reducing-bacteria-in-the-biogeochemical-cycle-of-arsenic-based-on-the-physiological-and-functional-analysis-of-aeromonas-sp-o23a
#8
Witold Uhrynowski, Klaudia Debiec, Aleksandra Sklodowska, Lukasz Drewniak
Dissimilatory arsenate reducing bacteria (DARB) are known to contribute to the mobilization of arsenic and other elements from minerals. Despite this, metabolic capabilities of only a few DARB strains have been thoroughly investigated so far, and the influence of these bacteria on the bioavailability of arsenic in the environment is still a topic for discussion. In this study, Aeromonas sp. O23A, one of the dominant DARB strains found in the Zloty Stok mine (SW Poland), was subjected to a detailed physiological and functional analysis aimed to identify the actual environmental impact of this strain...
April 25, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28454036/a-clean-environmental-week-let-the-nature-breathe
#9
Khaled Moustafa
High levels of CO2 emissions in the atmosphere and toxic pollutants in air, water and food have serious repercussions on all life's systems, including living beings, environment and economy. Everyone on the Earth is concerned by pollution in some way or another, no matter where and how the pollution is produced as airborne and foodborne pollutants could circulate around the world in different ways, through for example climate components (wind, rain) and/or import and export of foodstuffs. Similarly to living beings that take advantage of day-night circadian rhythms to recover after diurnal hardships, the environment in its entirety could also be seen as a complex living system that needs regular breaks to assimilate or ingest toxic pollutants produced during intensive and continuous industrial processes...
April 25, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28454030/pesticide-impact-study-in-the-peri-urban-horticultural-area-of-gran-la-plata-argentina
#10
Tomás M Mac Loughlin, Leticia Peluso, Damián J G Marino
Vegetable production systems are characterized by intense pesticide use, yet the effects on the surrounding environment are largely unknown and need to be studied. Given this knowledge gap, the objective of this work is to determine the impact of horticulture on a representative watercourse by conducting an integrated study of the occurrence and concentration of pesticides in bottom sediments and their relation to lethal and sublethal effects on benthic fauna. Two sampling campaigns were conducted during seasons of low and high pesticide application in five sites along the Carnaval creek, located in the peri-urban area of La Plata City (Buenos Aires, Argentina)...
April 25, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28454028/corrigendum-to-groundwater-arsenic-contamination-in-burkina-faso-west-africa-predicting-and-verifying-regions-at-risk-sci-total-environ-584-585-2017-958-970
#11
Anja Bretzler, Franck Lalanne, Julien Nikiema, Joel Podgorski, Numa Pfenninger, Michael Berg, Mario Schirmer
No abstract text is available yet for this article.
April 25, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28454026/textural-characteristics-and-sedimentary-environment-of-sediment-at-eroded-and-deposited-regions-in-the-severely-eroded-coastline-of-batu-pahat-malaysia
#12
Wan Hanna Melini Wan Mohtar, Siti Aminah Bassa Nawang, Khairul Nizam Abdul Maulud, Yannie Anak Benson, Wan Ahmad Hafiz Wan Mohamed Azhary
This study investigates the textural characteristics of sediments collected at eroded and deposited areas of highly severed eroded coastline of Batu Pahat, Malaysia. Samples were taken from systematically selected 23 locations along the 67km stretch of coastline and are extended to the fluvial sediments of the main river of Batu Pahat. Grain size distribution analysis was conducted to identify its textural characteristics and associated sedimentary transport behaviours. Sediments obtained along the coastline were fine-grained material with averaged mean size of 7...
April 24, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28454022/quantitative-analysis-of-adsorptive-interactions-of-ionic-and-neutral-pharmaceuticals-and-other-chemicals-with-the-surface-of-escherichia-coli-cells-in-aquatic-environment
#13
Chul-Woong Cho, Jeong-Soo Park, Yufeng Zhao, Yeoung-Sang Yun
Since Escherichia coli is ubiquitous in nature and has been applied to biological, chemical, and environmental processes, molecular-level understanding of adsorptive interactions between chemicals and the bacterial surface is of great importance. To characterise the adsorption properties of the surface of E. coli cells in aquatic environment, the binding affinities (log Kd) of calibration compounds were experimentally measured, and then based on the values and numerically well-defined molecular interaction forces, i...
April 25, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28454019/adsorption-of-carbon-dots-onto-al2o3-in-aqueous-experimental-and-theoretical-studies
#14
Xia Liu, Jiaxing Li, Xiaohua Wu, Zhi Zeng, Xianlong Wang, Tasawar Hayat, Xiaodong Zhang
Increasing interests in carbon dots (CDs) research resulted in the increased production of CDs and the risk to be released into the environment, including surface water and groundwater. To assess the environmental behavior of CDs, the stability of CDs in aqueous solutions were first examined by different environmental conditions, followed by the systematic investigation of the adsorption behaviors of CDs onto Al2O3 surface. Electrostatic interactions were considered as the dominant forces in CDs adsorption onto Al2O3 process, which were further confirmed by controlling experiments as compared with other minerals or oxides, such as montmorillonite, kaolinite and SiO2...
April 25, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28453985/realising-the-dream-of-becoming-a-nurse-underrepresented-bsc-nursing-students-experiences
#15
Brid O'Brien, Margaret M Graham, Deirdre O'Sullivan
BACKGROUND: This paper describes the experiences of underrepresented BSc nursing students in realising the dream of becoming a nurse in one university. In the past ten years, pre-registration nurse education has become established within higher education in Ireland. This development includes promoting access and inclusion of students from traditionally underrepresented groups in higher education. A third of nursing students currently access places on programmes through routes specifically designed for underrepresented groups...
April 18, 2017: Nurse Education Today
https://www.readbyqxmd.com/read/28453971/segmented-filamentous-bacteria-th17-inducers-and-helpers-in-a-hostile-world
#16
REVIEW
Pamela Schnupf, Valérie Gaboriau-Routhiau, Philippe J Sansonetti, Nadine Cerf-Bensussan
The Th17 cell composition in the murine gut is strikingly dependent on the presence of the commensal segmented filamentous bacteria (SFB). SFB potently stimulates innate and adaptive immune responses and protects the host from pathogens both in and outside of the gut, partly due to its unique ability to promote a Th17-fostering environment. Recent work has highlighted the role of the tight adherence of SFB to the intestinal surface in mediating the potent immunostimulatory potential of SFB. Progress has also been made in our understanding of how SFB fosters this protective immune environment on the cellular and molecular level...
April 25, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28453965/adsorption-behavior-and-mechanism-of-different-arsenic-species-on-mesoporous-mnfe2o4-magnetic-nanoparticles
#17
Qingsong Hu, Yuling Liu, Xueyuan Gu, Yaping Zhao
Arsenic pollution poses severe threat to human health, therefore dealing with the problem of arsenic contamination in water bodies is extremely important. The adsorption behaviors of different arsenic species, such as arsenate (As(V)), p-arsanilic acid (p-ASA), roxarsone (ROX), dimethylarsenate (DMA) from water using mesoporous bimetal oxide magnetic manganese ferrite nanoparticles (MnFe2O4) have been detailedly investigated. The adsorbent was synthesized via a facile co-precipitation approach and recovered conveniently owing to its strong magnetic properties...
April 18, 2017: Chemosphere
https://www.readbyqxmd.com/read/28453932/a-green-route-to-a-na2fepo4f-based-cathode-for-sodium-ion-batteries-of-high-rate-and-long-cycling-life
#18
Xiang Deng, Wenxiang Shi, Jaka Sunarso, Meilin Liu, Zongping Shao
Sodium-ion batteries (SIBs) are considered one of the most promising alternatives for large-scale energy storage due largely to the abundance and low cost of sodium. However, the lack of high-performance cathode materials at low cost represents a major obstacle towards broad commercialization of SIB technology. In this work, we report a green route strategy that allows cost-effective fabricaiton of carbon-coated Na2FePO4F cathode for SIBs. By using vitamin C as a green organic carbon source and environment-friendly water-based polyacrylic latex as the binder, we have demonstrated that the Na2FePO4F phase in the as-derived Na2FePO4F/C electrode shows a high reversible capacity of 117 mAh g-1 at a cycling rate of 0...
April 28, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28453914/the-shape-of-things-to-come-the-formation-of-modulated-nematic-mesophases-at-various-length-scales
#19
Richard J Mandle
The twist-bend nematic phase is a recently discovered liquid-crystalline phase that exhibits macroscopic chirality even when formed from achiral materials, and as such presents a unique testbed for studies concerning the spontaneous breaking of mirror symmetry in soft matter. It is primarily exhibited by materials whose molecular structure is composed of two rigid aromatic units (such as biphenyl connected by a flexible spacer). The local structure of the NTB phase is nematic-like - with molecules having an average orientational order but no positional order - with a nanoscale helix where the pitch is of the order of several nanometres...
April 28, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28453855/newly-characterized-interaction-stabilizes-dna-structure-oligoethylene-glycols-stabilize-g-quadruplexes-ch-%C3%AF-interactions
#20
Hisae Tateishi-Karimata, Tatsuya Ohyama, Takahiro Muraoka, Peter Podbevsek, Adam M Wawro, Shigenori Tanaka, Shu-Ichi Nakano, Kazushi Kinbara, Janez Plavec, Naoki Sugimoto
Oligoethylene glycols are used as crowding agents in experiments that aim to understand the effects of intracellular environments on DNAs. Moreover, DNAs with covalently attached oligoethylene glycols are used as cargo carriers for drug delivery systems. To investigate how oligoethylene glycols interact with DNAs, we incorporated deoxythymidine modified with oligoethylene glycols of different lengths, such as tetraethylene glycol (TEG), into DNAs that form antiparallel G-quadruplex or hairpin structures such that the modified residues were incorporated into loop regions...
April 27, 2017: Nucleic Acids Research
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