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https://www.readbyqxmd.com/read/29203960/monascus-purpureus-induced-apoptosis-on-gastric-cancer-cell-by-scavenging-mitochondrial-reactive-oxygen-species
#1
Hiromi Kurokawa, Hiromu Ito, Hirofumi Matsui
Monascus purpureus is a red dye derived from yeast rice and has been used as color additives for food in East Asia. Monascus purpureus consists of several bioactive components. Some of these components work as a radical scavenger, thus monascus purpureus would also eliminate reactive oxygen species. Cancer cells maintain the high level of reactive oxygen species than normal cell and are death by imbalance in pro-oxidant/antioxidant homeostasis. In this study, we investigated whether monascus purpureus induced cancer specific cell death by scavenging reactive oxygen species...
November 2017: Journal of Clinical Biochemistry and Nutrition
https://www.readbyqxmd.com/read/29198118/rapid-and-quantitative-detection-of-vibrio-parahemolyticus-by-the-mixed-dye-based-loop-mediated-isothermal-amplification-assay-on-a-self-priming-compartmentalization-microfluidic-chip
#2
Bo Pang, Xiong Ding, Guoping Wang, Chao Zhao, Yanan Xu, Kaiyue Fu, Jingjing Sun, Xiuling Song, Wenshuai Wu, Yushen Liu, Qi Song, Jiumei Hu, Juan Li, Ying Mu
Vibrio parahemolyticus (VP) mostly isolated from aquatic products is one of the major causes of bacterial food poisoning events worldwide, which could be reduced using a promising on-site detection method. Herein, a rapid and quantitative method for VP detection was developed by applying mixed-dye-loaded loop-mediated isothermal amplification (LAMP) assay on self-priming compartmentalization (SPC) microfluidic chip, termed on-chip mixed-dye-based LAMP (CMD-LAMP). Compared to conventional approaches, CMD-LAMP was advantageous on the limit of detection which reached down to 1×103 CFU/ mL in food contaminated samples without the pre-enrichment of bacteria...
December 3, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29197541/structural-identification-and-biological-activity-of-six-new-shellolic-esters-from-lac
#3
Jin Lu, Lei Shang, Huimin Wen, Jian Huang, Guoyu Li, Jinhui Wang
Six new sesquiterpenoid esters, named Shellolic ester A-F (1-6), along with four known Lac dyes (7-10) were isolated from methanol extract of the secretions of Laccifer lacca. Their structures were established on the basis of spectroscopic analyses (IR, HR-ESI-MS, 1D and 2D NMR) and by comparison with published data. Biological activities evaluation of the isolates showed that they were inactive against human cancer cell lines (HepG2, MCF-7, Hela and C6) and LPS-treated RAW264.7, which is well consistent with that Lac resin used as nontoxic material in agriculture applications, pharmaceutical formulations, and food additives...
November 29, 2017: Fitoterapia
https://www.readbyqxmd.com/read/29196009/stability-of-curcumin-encapsulated-in-solid-lipid-microparticles-incorporated-in-cold-set-emulsion-filled-gels-of-soy-protein-isolate-and-xanthan-gum
#4
Thais C Brito-Oliveira, Marina Bispo, Izabel C F Moraes, Osvaldo H Campanella, Samantha C Pinho
The objective of this study was to investigate the feasibility of producing cold-set emulsion filled gels (EFG), using soy protein isolate (SPI) and xanthan gum (XG) and incorporating curcumin-loaded solid lipid microparticles (SLM). For this purpose, the formulation GXG (15%, w/v SPI, 0.1%, w/v XG and 5mM CaCl2) was selected for the production of EFG. A comparative study on the rheological and microstructural properties of non-filled gels and EFG revealed that SLM stabilized with Tween 80-Span 80 behaved as active fillers in the gel matrix, increasing the Young's modulus from 1...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29193817/isolation-and-purification-of-food-grade-c-phycocyanin-from-arthrospira-platensis-and-its-determination-in-confectionery-by-hplc-with-diode-array-detection
#5
Maria Kissoudi, Ioannis Sarakatsianos, Victoria Samanidou
C-Phycocyanin is the major phycobiliprotein in Arthrospira platensis, also known as Spirulina, which is a cyanobacterium used as a dietary supplement because of its powerful effects on body and brain. C-phycocyanin is a blue-colored accessory photosynthetic pigment with multiple applications in food industry as natural dye or additive, and in pharmaceuticals. This study presents a simple protocol for the extraction and purification of food-grade C-phycocyanin from Arthrospira platensis. The cell lysis of cyanobacterium was performed by sonication combined with repeated freezing and thawing cycles...
November 28, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29180052/evaluation-of-thiazolidinone-derivatives-as-a-new-class-of-mushroom-tyrosinase-inhibitors
#6
Mehrnaz Rezaei, Hamed Taj Mohammadi, Atiyeh Mahdavi, Mostafa Shourian, Hossein Ghafoori
Tyrosinase (EC 1.14.18.1) is a key copper-containing metalloenzyme widely distributed in nature and plays determinant role in melanin biosynthesis. The enzyme manifests two unusual catalytic properties including oxidase and monooxygenase activities. Its inhibitors may be applied to efficiently treat of hyperpigmentation and widely used in pharmaceutical and cosmetic products, as well as food supplements and insecticides. The present study aims to evaluate the inhibitory effects of some novel azo-hydrazone tautomeric dyes (4a-e) including bioactive thiazolidinone moiety on the activity of the mushroom tyrosinase...
November 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29175285/development-of-hybeacon%C3%A2-probes-for-specific-mrna-detection-using-body-fluids-as-a-model-system
#7
Beccy Stafford-Allen, Nick Dawnay, Erin K Hanson, Glyn Ball, Ambika Gupta, Stephen Blackman, David J French, Nicola Duxbury, Jack Ballantyne, Simon Wells
HyBeacons are linear oligonucleotides which incorporate fluorescent dyes covalently linked to internal nucleotides. They have previously been used with PCR and isothermal amplification to interrogate SNPs and STRs in fields as diverse as clinical diagnostics, food authentication, and forensic DNA profiling. This work explores their use for the identification of expressed gene sequences through mRNA profiling. The use of mRNA is becoming increasingly common in forensic casework to identify body fluids on evidence items, as it offers higher specificity and fewer false positives than current chemical presumptive testing methods...
November 22, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29166270/detection-of-yersinia-enterocolitica-species-in-pig-tonsils-and-raw-pork-meat-by-the-real-time-pcr-and-culture-methods
#8
M A Stachelska
The aim of the present study was to establish a rapid and accurate real-time PCR method to detect pathogenic Yersinia enterocolitica in pork. Yersinia enterocolitica is considered to be a crucial zoonosis, which can provoke diseases both in humans and animals. The classical culture methods designated to detect Y. enterocolitica species in food matrices are often very time-consuming. The chromosomal locus _tag CH49_3099 gene, that appears in pathogenic Y. enterocolitica strains, was applied as DNA target for the 5' nuclease PCR protocol...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29163827/fluorescence-contrast-enhanced-proliferative-lesion-imaging-by-enema-administration-of-indocyanine-green-in-a-rat-model-of-colon-carcinogenesis
#9
Nobuhiko Onda, Reiko Mizutani-Morita, Susumu Yamashita, Rei Nagahara, Shinya Matsumoto, Toshinori Yoshida, Makoto Shibutani
The fluorescent contrast agent indocyanine green (ICG) is approved by the Food and Drug Administration for clinical applications. We previously reported that cultured human colon tumor cells preferentially take up ICG by endocytic activity in association with disruption of their tight junctions. The present study explored ICG availability in fluorescence imaging of the colon to identify proliferative lesions during colonoscopy. The cellular uptake of ICG in cultured rat colon tumor cells was examined using live-cell imaging...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137768/low-frequency-ultrasonic-assisted-hydrolysis-of-starch-in-the-presence-of-%C3%AE-amylase
#10
Anne Gaquere-Parker, Tamera Taylor, Raihannah Hutson, Ashley Rizzo, Aubrey Folds, Shastina Crittenden, Neelam Zahoor, Bilal Hussein, Aaron Arruda
Hydrolysis of starch is an important process in the food industry and in the production of bioethanol or smaller carbohydrate molecules that can be used as starting blocks for chemical synthesis. Such hydrolysis can be enhanced by lowering the pH, heating the reaction mixture or catalyzing the reaction with enzymes. This study reports the effect of sonication on the reaction rate of starch hydrolysis at different temperatures, in the presence or absence of alpha-amylase. Starch Azure, a commercially available potato starch covalently linked with Remazol Brilliant Blue, has been chosen since its hydrolysis releases a blue dye, which concentration can be monitored by UV Vis spectroscopy...
March 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29130832/developmental-toxicity-assay-for-food-additive-tartrazine-using-zebrafish-danio-rerio-embryo-cultures
#11
Vani Joshi, Pancharatna Katti
Tartrazine (TTZ) is an azo dye used as a colorant in food products, drugs, and cosmetics. The present study evaluates the impacts of TTZ on embryonic development of zebrafish ( Danio rerio). Laboratory-raised D. rerio embryos (n = 20/concentration) were exposed to graded dilutions of TTZ (0, 0.1, 1, 2, 3, 4, 5, 10, 20, 30, 40, 50, 75, and 100 mM) from gastrulation stage (5.25 hours postfertilization [hpf]) until hatching and developmental trajectory was traced up to day 7. The no observed effect concentration (NOEC), median lethal concentration (LC50), median effective concentration (EC50), and teratogenic index (TI) were calculated...
January 1, 2017: International Journal of Toxicology
https://www.readbyqxmd.com/read/29128093/miniaturized-matrix-solid-phase-dispersion-followed-by-liquid-chromatography-tandem-mass-spectrometry-for-the-quantification-of-synthetic-dyes-in-cosmetics-and-foodstuffs-used-or-consumed-by-children
#12
Eugenia Guerra, Maria Llompart, Carmen Garcia-Jares
Miniaturized matrix solid-phase dispersion (MSPD) followed by liquid chromatography tandem mass spectrometry (LC-MS/MS) has been proposed for the simultaneous analysis of different classes of synthetic dyes in confectionery and cosmetics intended for or mostly consumed by children. Selected compounds include most of the permitted dyes as food additives as well as some of the most frequently used to color cosmetic products in accordance with the respective European directives. MSPD procedure was optimized by means of experimental design, allowing an effective, rapid and simple extraction of dyes with low sample and reagents consumption (0...
October 27, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29127809/optimisation-of-the-antifungal-potency-of-the-amidated-peptide-h-orn-orn-trp-trp-nh2-against-food-contaminants
#13
Thibaut Thery, Yvonne O'Callaghan, Nora O'Brien, Elke K Arendt
The design of novel efficient antimicrobial peptides (AMPs) faces several issues, such as cost of synthesis, proteolytic stability or cytotoxicity. The identification of key determinants involved in the activity of AMPs, such as cationicity and amphipathicity, allowed the synthesis of short peptides with optimized properties. An ultrashort peptide made of the sequence H-Orn-Orn-Trp-Trp-NH2 (O3TR) showed antifungal activity against several contaminants from food products. This peptide inhibited the growth of the filamentous fungi Fusarium culmorum, Penicillium expansum and Aspergillus niger within a range of concentration of 12...
November 8, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29125493/validation-of-ultrasensitive-mutant-huntingtin-detection-in-human-cerebrospinal-fluid-by-single-molecule-counting-immunoassay
#14
Valentina Fodale, Roberto Boggio, Manuel Daldin, Cristina Cariulo, Maria Carolina Spiezia, Lauren Mary Byrne, Blair R Leavitt, Edward Wild, Douglas Macdonald, Andreas Weiss, Alberto Bresciani
BACKGROUND: The measurement of disease-relevant biomarkers has become a major component of clinical trial design, but in the absence of rigorous clinical and analytical validation of detection methodology, interpretation of results may be misleading. In Huntington's disease (HD), measurement of the concentration of mutant huntingtin protein (mHTT) in cerebrospinal fluid (CSF) of patients may serve as both a disease progression biomarker and a pharmacodynamic readout for HTT-lowering therapeutic approaches...
November 9, 2017: Journal of Huntington's Disease
https://www.readbyqxmd.com/read/29118054/vitamin-d-status-in-chronic-fatigue-syndrome-myalgic-encephalomyelitis-a-cohort-study-from-the-north-west-of-england
#15
Kate E Earl, Giorgos K Sakellariou, Melanie Sinclair, Manuel Fenech, Fiona Croden, Daniel J Owens, Jonathan Tang, Alastair Miller, Clare Lawton, Louise Dye, Graeme L Close, William D Fraser, Anne McArdle, Michael B J Beadsworth
OBJECTIVE: Severe vitamin D deficiency is a recognised cause of skeletal muscle fatigue and myopathy. The aim of this study was to examine whether chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is associated with altered circulating vitamin D metabolites. DESIGN: Cohort study. SETTING: UK university hospital, recruiting from April 2014 to April 2015. PARTICIPANTS: Ninety-two patients with CFS/ME and 94 age-matched healthy controls (HCs)...
November 8, 2017: BMJ Open
https://www.readbyqxmd.com/read/29116354/functional-electrospun-nanofibers-for-multimodal-sensitive-detection-of-biogenic-amines-in-food-via-a-simple-dipstick-assay
#16
Nadezhda S Yurova, Alexandra Danchuk, Sarah N Mobarez, Nongnoot Wongkaew, Tatiana Rusanova, Antje J Baeumner, Axel Duerkop
Electrospun nanofibers (ENFs) are promising materials for rapid diagnostic tests like lateral flow assays and dipsticks because they offer an immense surface area while excluding minimal volume, a variety of functional surface groups, and can entrap functional additives within their interior. Here, we show that ENFs on sample pads are superior in comparison to standard polymer membranes for the optical detection of biogenic amines (BAs) in food using a dipstick format. Specifically, cellulose acetate (CA) fibers doped with 2 mg/mL of the chromogenic and fluorogenic amine-reactive chameleon dye Py-1 were electrospun into uniform anionic mats...
November 7, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29105097/ultrafine-fibers-of-zein-and-anthocyanins-as-natural-ph-indicator
#17
Luciana Prietto, Vania Zanella Pinto, Shanise Lisie Mello El Halal, Michele Greque de Morais, Jorge Alberto Vieria Costa, Loong-Tak Lim, Alvaro Renato Guerra Dias, Elessandra da Rosa Zavareze
BACKGROUND: pH-sensitive indicator membranes, useful for pharmaceutical, food, and packaging applications, can be formed by encapsulating halochromic compounds within various solid supports. To this end, electrospinning is a versatile technique for the development of these indicators, by entrapping pH dyes within ultrafine polymer fibers. RESULTS: The ultrafine zein fibers, containing 5% (w/v) anthocyanins, had an average diameter of 510 nm. The pH-sensitive membrane exhibited color changes from pink to green, when exposed to acidic and alkaline buffers, respectively...
November 3, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29101062/antibacterial-and-antibiofilm-activities-of-eugenol-from-essential-oil-of-syzygium-aromaticum-l-merr-l-m-perry-clove-leaf-against-periodontal-pathogen-porphyromonas-gingivalis
#18
Yi Zhang, Yue Wang, Xiaojing Zhu, Ping Cao, Shaomin Wei, Yanhua Lu
The antibacterial effect and mechanism of eugenol from Syzygium aromaticum (L.) Merr. & L. M. Perry (clove) leaf essential oil (CLEO) against oral anaerobe Porphyromonas gingivalis were investigated. The results showed that eugenol, with content of 90.84% in CLEO, exhibited antibacterial activity against P. gingivalis at a concentration of 31.25 μM. Cell shrink and lysis caused by eugenol were observed with Scanning Electron Microscopy (SEM). The release of macromolecules and uptake of fluorescent dye indicated that the antibacterial activity was due to the ability of eugenol to permeabilize the cell membrane and destroy the integrity of plasmatic membrane irreversibly...
October 31, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29090907/colorimetric-nucleic-acid-detection-on-paper-microchip-using-loop-mediated-isothermal-amplification-and-crystal-violet-dye
#19
Sharmili Roy, Noor Faizah Mohd-Naim, Mohammadali Safavieh, Minhaz Uddin Ahmed
Nucleic acid detection is of paramount importance in monitoring of microbial pathogens in food safety and infectious disease diagnostic applications. To address these challenges, a rapid, cost-effective label-free technique for nucleic acid detection with minimal instrumentations is highly desired. Here, we present paper microchip to detect and quantify nucleic acid using colorimetric sensing modality. The extracted DNA from food samples of meat as well as microbial pathogens was amplified utilizing loop-mediated isothermal amplification (LAMP)...
November 10, 2017: ACS Sensors
https://www.readbyqxmd.com/read/29083531/identification-of-genes-involved-in-flavonoid-biosynthesis-in-sophora-japonica-through-transcriptome-sequencing
#20
Fu-Sheng Zhang, Qian-Yu Wang, Ya-Jie Pu, Tong-Yao Chen, Xue-Mei Qin, Jie Gao
Sophora japonica is a traditional Chinese medicinal ingredient that is widely used in the medicine, food, and industrial dye industries. Since flavonoids are the main components of S. japonica, studying the flavonoid composition and content of this plant is important. This study aimed to identify molecules involved in the flavonoid biosynthetic pathways in S. japonica. Deep sequencing was performed, and 85,877,352 clean reads were filtered from 86,095,152 raw reads. The clean reads were spliced to obtain 111,382 unigenes, which were then annotated with NR, GO, KEGG, eggNOG...
October 30, 2017: Chemistry & Biodiversity
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