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https://www.readbyqxmd.com/read/29665689/the-human-sweet-receptor-t1r3-is-functional-in-human-gastric-parietal-tumor-cells-hgt-1-and-modulates-cyclamate-and-acesulfame-k-induced-mechanisms-of-gastric-acid-secretion
#1
Muhammet Zopun, Kathrin I Liszt, Verena Stoeger, Maik Behrens, Ulrike Redel, Jakob P Ley, Joachim Hans, Veronika Somoza
The non-caloric sweeteners (NCS) cyclamate (Cycl) and acesulfame K (AceK) are widely added to foods and beverages. Little is known about their impact on gastric acid secretion (GAS), which is stimulated by dietary protein and bitter-tasting compounds. Since Cycl and AceK have a bitter off-taste in addition to their sweet-taste, we hypothesized they modulate mechanisms of GAS in human gastric parietal cells (HGT-1). HGT-1 cells were exposed to sweet tastants (50 mM of glucose, D-threonine, Cycl or AceK), and analyzed for their intracellular pH index (IPX), as indicator of proton secretion by means of a pH-sensitive dye, and for mRNA-levels of GAS-associated genes by RT-qPCR...
April 17, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29665473/magnetic-metal-organic-frameworks-for-fast-and-efficient-solid-phase-extraction-of-six-sudan-dyes-in-tomato-sauce
#2
Xin-Ran Shi, Xue-Lei Chen, Yu-Lan Hao, Li Li, Hou-Jun Xu, Man-Man Wang
Magnetic solid-phase extraction is an effective and useful technique to preconcentrate trace analytes from food samples. In this study, a magnetic trimeric chromium octahedral metal-organic framework (Fe3 O4 -NH2 @MIL-101) was fabricated and characterized. Fe3 O4 -NH2 @MIL-101 was applied as an adsorbent of magnetic solid-phase extraction combined with high performance liquid chromatography to effectively isolate and simultaneously determine six Sudan dyes (Para Red, Sudan I-IV, and Sudan Red 7B) from tomato sauce...
April 10, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29665023/propidium-monoazide-real-time-loop-mediated-isothermal-amplification-for-specific-visualization-of-viable-salmonella-in-food
#3
Jiehong Fang, Yingying Wu, Daofeng Qu, Biao Ma, Xiaoping Yu, Mingzhou Zhang, Jianzhong Han
Traditional DNA-based molecular detection assays, including loop-mediated isothermal amplification (LAMP), are unable to differentiate between viable and dead cells. We developed a rapid and accurate method for detection of viable Salmonella cells in food using calcein dyed visual real-time LAMP combined with propidium monoazide (PMA) treatment. Amplification of DNA from up to 6.3 × 105 CFU ml-1 dead Salmonella cells was inhibited by 5 μmol l-1 PMA dye, whereas the DNA derived from viable cells were detected according the invA gene...
April 17, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29652135/low-cost-and-simple-fabrication-of-nanoplasmonic-paper-for-coupled-chromatography-separation-and-surface-enhanced-raman-detection
#4
Joshua D Weatherston, Ricci Kirsten O Seguban, Dawson Hunt, Hung-Jen Wu
Surface-enhanced Raman scattering (SERS) is a powerful analytical tool which enables the detection and identification of analytes adsorbed on nanostructured noble metals. However, SERS analysis of complex mixtures can be challenging due to spectral overlap and interference. In this report, we demonstrate a method to simplify the identification of mixed-analyte samples by coupling SERS detection with chromatographic separation on a nanoplasmonic paper substrate. This "nanopaper" substrate is a silver coated glass microfiber filter paper which possesses large SERS enhancement and can serve as a stationary phase in paper chromatography...
April 13, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29651377/renoprotective-effect-of-cinnamaldehyde-in-food-color-induced-toxicity
#5
Uma Kant Sharma, Ramesh Kumar, Ashutosh Gupta, Risha Ganguly, Abhay K Pandey
Present study reports the effects of metanil yellow, a non-permitted food colouring dye, on the biomarkers of oxidative stress and kidney function in blood and renal tissue of albino Wistar rats and its mitigation by cinnamaldehyde, a major phytoconstituents of cinnamon. Oral administration of metanil yellow in rats caused about 70% reduction in ferric reducing ability (FRAP 5.1 μM/L) and 50% decline in reduced glutathione (GSH 59.27 nM/mg protein) content in plasma with simultaneous increase in serum creatinine level...
April 2018: 3 Biotech
https://www.readbyqxmd.com/read/29642832/-determination-of-the-role-of-salmonella-enterica-plasmids-in-antibiotic-susceptibility-and-caenorhabditis-elegans-pathogenicity
#6
Deniz Aksoy, Ece Şen
Poultry animals and poultry associated products are important risk sources for Salmonellosis. S.Kentucky and S.Infantis are among the serovars frequently isolated from retail chickens and were reported to be isolated in Turkey. In this study, the role of plasmids carried by S.Kentucky and S.Infantis isolates in antibiotic resistance profiles of the isolates and their pathogenicity on Caenorhabditis elegans nematode model system were investigated. The isolates used, 1 of Kentucky and 2 of Infantis serotypes, were selected among food-borne Salmonella isolated from chicken carcass in Edirne...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29629617/color-matters-they-would-choose-if-they-could-see
#7
Tina Nitezki, Nadja Schulz, Stephanie Krämer
Concerning standardization of laboratory animal husbandry, only exiguous changes of habitat can potentially influence animal physiology or results of behavioral tests. Routinely, mice chow is dyed when different types of diets are dispensed. Given the fact that the dye itself has no effects on food odor or flavor, we wanted to test the hypothesis that the color of chow has an impact on food uptake in mice. Twelve-week-old male mice of different strains (C57BL/6J, DBA/2J, C3H/HeJ, BALB/cJ; n = 12/strain) were single-housed in PhenoMaster® cages...
January 1, 2018: Laboratory Animals
https://www.readbyqxmd.com/read/29627251/a-selective-medium-for-the-enumeration-and-differentiation-of-lactobacillus-delbrueckii-ssp-bulgaricus
#8
Nwadiuto O Nwamaioha, Salam A Ibrahim
Modified reinforced clostridial medium (mRCM) was developed and evaluated for the differential enumeration of Lactobacillus delbrueckii ssp. bulgaricus. Lactobacillus bulgaricus, an important species of lactic acid bacteria with health benefits, is used in the production of yogurt and other fermented foods. Our results showed that supplementing reinforced clostridial medium with 0.025% CaCl2 , 0.01% uracil, and 0.2% Tween 80 (mRCM) significantly enhanced the growth rate of L. bulgaricus RR and ATCC 11842 strains as measured by the optical densities of these strains after 12 h of incubation at 42°C...
April 4, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29626132/shortwave-infrared-fluorescence-imaging-with-the-clinically-approved-near-infrared-dye-indocyanine-green
#9
Jessica A Carr, Daniel Franke, Justin R Caram, Collin F Perkinson, Mari Saif, Vasileios Askoxylakis, Meenal Datta, Dai Fukumura, Rakesh K Jain, Moungi G Bawendi, Oliver T Bruns
Fluorescence imaging is a method of real-time molecular tracking in vivo that has enabled many clinical technologies. Imaging in the shortwave IR (SWIR; 1,000-2,000 nm) promises higher contrast, sensitivity, and penetration depths compared with conventional visible and near-IR (NIR) fluorescence imaging. However, adoption of SWIR imaging in clinical settings has been limited, partially due to the absence of US Food and Drug Administration (FDA)-approved fluorophores with peak emission in the SWIR. Here, we show that commercially available NIR dyes, including the FDA-approved contrast agent indocyanine green (ICG), exhibit optical properties suitable for in vivo SWIR fluorescence imaging...
April 6, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29623249/therapeutic-applications-of-bacterial-pigments-a-review-of-current-status-and-future-opportunities
#10
REVIEW
Muhammad Numan, Samina Bashir, Roqayya Mumtaz, Sibgha Tayyab, Najeeb Ur Rehman, Abdul Latif Khan, Zabta Khan Shinwari, Ahmed Al-Harrasi
Non-toxicity, biodegradability and non-carcinogenicity of the natural pigments, dyes and colorants make them an attractive source for human use. Bacterial pigments are colored metabolites secreted by bacteria under stress. The industrial uses of bacterial pigments have increased many folds because of several advantages over the synthetic pigments. Among natural resources, bacterial pigments are mostly preferred because of simple culturing and pigment extraction techniques, scaling up and being time economical...
April 2018: 3 Biotech
https://www.readbyqxmd.com/read/29614728/diatomite-photonic-crystals-for-facile-on-chip-chromatography-and-sensing-of-harmful-ingredients-from-food
#11
Xianming Kong, Qian Yu, Erwen Li, Rui Wang, Qing Liu, Alan X Wang
Diatomaceous earth-otherwise called diatomite-is essentially composed of hydrated biosilica with periodic nanopores. Diatomite is derived from fossilized remains of diatom frustules and possesses photonic-crystal features. In this paper, diatomite simultaneously functions as the matrix of the chromatography plate and the substrate for surface-enhanced Raman scattering (SERS), by which the photonic crystal-features could enhance the optical field intensity. The on-chip separation performance of the device was confirmed by separating and detecting industrial dye (Sudan I) in an artificial aqueous mixture containing 4-mercaptobenzoic acid (MBA), where concentrated plasmonic Au colloid was casted onto the analyte spot for SERS measurement...
March 31, 2018: Materials
https://www.readbyqxmd.com/read/29596843/viability-assessment-of-primary-growth-oocytes-following-ovarian-tissue-vitrification-of-neotropical-teleost-pacu-piaractus-mesopotamicus
#12
Lis S Marques, Ana A Fossati, Martinha S Leal, Rômulo B Rodrigues, Robie A Bombardelli, Danilo P Streit
Vitrification of ovarian tissue containing immature oocytes provides an important tool for protecting the endangered species and genetic diversity in aquatic species. Therefore, the main objective was to assess primary growth (PG) oocytes viability following ovarian tissue vitrification using histological analysis, two staining protocols (trypan blue or fluorescein diacetate combined with propidium iodide) and mitochondrial activity assay (MTT assay). In addition, oocyte histomorphometry was performed to evaluate the morphometric parameters after vitrification and the relationship with the occurrence of damage (nucleus and/or membrane) in PG oocytes...
March 26, 2018: Cryobiology
https://www.readbyqxmd.com/read/29595074/alleviation-of-hippocampal-endoplasmic-reticulum-stress-by-allomyrina-dichotoma-larvae-extract
#13
Jongwan Kim, Md Nazmul Haque, Tae-Won Goo, Il Soo Moon
In the brain, endoplasmic reticulum (ER) stress results in synaptic dysfunction and eventually leads to neurodegeneration. Allomyrina dichotoma larvae are a Chinese ethnomedicine and are widely used in East Asia. In the present study, we investigated the ability of ethanol extract of A. dichotoma larvae (ADE) to improve synaptic structure and function by activating unfolded protein response (UPR) under ER stress in animal and neuron culture models. ER stress was induced in obese mice fed a high fat diet (HFD) or by treating dissociated cultures of rat embryonic (E19) hippocampal neurons with tunicamycin (TM)...
March 29, 2018: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/29590133/the-effect-of-pond-dyes-on-oviposition-and-survival-in-wild-uk-culex-mosquitoes
#14
Natali Ortiz-Perea, Rebecca Gander, Oliver Abbey, Amanda Callaghan
British Culex pipiens complex [Culex pipiens sensu lato) mosquito distribution, abundance, and potential for disease transmission are intimately linked to their environment. Pond and lake dyes that block light to restrict algal photosynthesis are a relatively new product assumed to be an environmentally friendly since they are based on food dyes. Their use in urban garden ponds raises questions linked to mosquito oviposition, since coloured water can be an attractant. Culex (mostly pipiens) is commonly found in UK gardens and is a potential vector of viruses including the West Nile Virus (WNV)...
2018: PloS One
https://www.readbyqxmd.com/read/29576056/brazilin-and-caesalpinia-sappan-l-extract-protect-epidermal-keratinocytes-from-oxidative-stress-by-inducing-the-expression-of-gpx7
#15
Hyung Seo Hwang, Joong Hyun Shim
Caesalpinia sappan L., belonging to the family Leguminosae, is a medicinal plant that is distributed in Southeast Asia. The dried heartwood of this plant is used as a traditional ingredient of food, red dyes, and folk medicines in the treatment of diarrhea, dysentery, tuberculosis, skin infections, and inflammation. Brazilin is the major active compound, which has exhibited various pharmacological effects, including anti-platelet activity, anti-hepatotoxicity, induction of immunological tolerance, and anti-inflammatory and antioxidant activities...
March 2018: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29561885/antimicrobial-evaluation-of-red-phytoalexin-rich-sorghum-food-biocolorant
#16
Folachodé U G Akogou, Heidy M W den Besten, A P Polycarpe Kayodé, Vincenzo Fogliano, Anita R Linnemann
Sorghum (Sorghum bicolor) extract is traditionally used as red biocolorant in West Africa to colour foods, among which wagashi, a soft cheese. This biocolorant is a source of the phytoalexin apigeninidin and phenolic acids, and users claim that it has preservative effects next to its colouring properties. If such a claim can be scientifically substantiated, it adds a valuable functional property to this natural red colorant, thereby increasing its potential applications in the food industry. Hence, the present study evaluated the antimicrobial properties of dye sorghum extracts using challenge tests in broth and wagashi as a model of a popular food application...
2018: PloS One
https://www.readbyqxmd.com/read/29553257/perspective-on-the-ongoing-replacement-of-artificial-and-animal-based-dyes-with-alternative-natural-pigments-in-foods-and-beverages
#17
Ralf M Schweiggert
This perspective highlights current trends, advances, and challenges related to the replacement of artificial dyes and the insect-based carmine with alternative natural pigments. Briefly reviewing the history of food coloration, key publications and public events leading to diverse concerns about artificial dyes and carmine will be summarized. An overview about promising alternatives in the market and those under development is provided, including a separate section on coloring foodstuffs. The perspective aims at supporting readers to keep abreast with the enormous efforts undertaken by the food and beverage industry to replace certain food dyes...
March 19, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29548448/high-effective-adsorption-removal-of-illegal-food-dyes-from-contaminated-aqueous-solution-by-zr-mofs-uio-67
#18
Qingfeng Yang, Yue Wang, Jing Wang, Fangbing Liu, Na Hu, Hanna Pei, Weixia Yang, Zhonghong Li, Yourui Suo, Jianlong Wang
UiO-67, a bifunctional adsorbent, was successfully applied to remove illegal food dyes (Congo red and Malachite green) from aqueous solution. The relevant adsorption conditions were optimized: pH 6, 120 min for contact time, and 10.0 mg L-1 adsorbent dose for Congo red. Adsorption behavior of UiO-67 exhibits a better fitting to pseudo-second-order kinetic model and Langmuir model. Moreover, the maximum adsorption capacities of Congo red (1236.9 mg g-1 ) and Malachite green (357.3 mg g-1 ) are higher than that of the most reported adsorbents...
July 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29547777/a-versatile-ceo-2-co-3-o-4-coated-mesh-for-food-wastewater-treatment-simultaneous-oil-removal-and-uv-catalysis-of-food-additives
#19
Ya'nan Liu, Na Liu, Yuning Chen, Weifeng Zhang, Ruixiang Qu, Qingdong Zhang, Tzungyu-Shih, Lin Feng, Yen Wei
Food waste water is one of the most urgent environmental problems for the close connection between food and our daily life. Herein, we use a simple hydrothermal method to prepare a highly efficient catalyst-CeO2 /Co3 O4 compound on the stainless steel mesh, aiming for food waste water treatment. Possessing the superhydrophilic property and catalytic ability under ultraviolet light, CeO2 /Co3 O4 coated mesh has successfully processed three representative contaminants in food wastewater, which are soybean oil (food oil), AR (food dye) and VA (food flavor) simultaneously with an one-step filtration...
March 9, 2018: Water Research
https://www.readbyqxmd.com/read/29543720/determination-of-food-oxalates-using-silica-titania-xerogel-modified-with-eriochrome-cyanine-r
#20
Maria A Morosanova, Zahar V Samodelov, Elena I Morosanova
The interaction of silica-titania xerogel with triphenylmethane dyes (pyrocatechol violet, chrome azurol S, eriochrome cyanine R) has been investigated to create a new sensor material for solid phase spectrophotometric determination of food oxalates. The complex forming reaction between xerogel incorporated titanium(IV) and triphenylmethane dyes has been studied; half-reaction periods, complex composition, equilibrium constants, and xerogel sorption capacity have been calculated for each dye. Eriochrome cyanine R (ECR) is characterized by the shortest half-reaction period, the smallest equilibrium constant, and the greatest capacity; it has been chosen for the sensor material construction because titanium(IV)-ECR complex is formed faster and can be destroyed easier than other studied complexes...
March 15, 2018: Sensors
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