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https://www.readbyqxmd.com/read/28635650/the-application-of-heptamethine-cyanine-dye-dz-1-and-indocyanine-green-for-imaging-and-targeting-in-xenograft-models-of-hepatocellular-carcinoma
#1
Caiqin Zhang, Yong Zhao, He Zhang, Xue Chen, Ningning Zhao, Dengxu Tan, Hai Zhang, Changhong Shi
Near infrared fluorescence (NIRF) imaging has strong potential for widespread use in noninvasive tumor imaging. Indocyanine green (ICG) is the only Food and Drug Administration (FDA) -approved NIRF dye for clinical diagnosis; however, it is unstable and poorly targets tumors. DZ-1 is a novel heptamethine cyanine NIRF dye, suitable for imaging and tumor targeting. Here, we compared the fluorescence intensity and metabolism of DZ-1 and ICG. Additionally, we assayed their specificities and abilities to target tumor cells, using cultured hepatocellular carcinoma (HCC) cell lines, a nude mouse subcutaneous xenograft model of liver cancer, and a rabbit orthotopic transplantation model...
June 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28602300/development-of-on-line-spectrofluorimetric-methodology-for-selenium-monitoring-in-foods-and-biological-fluids-using-chrome-azurol-s-quenching
#2
Débora G Santarossa, Liliana P Fernández
A novel, simple and accurate on-line green methodology for Se(IV) monitoring by molecular fluorescence has been developed. Because the analyte does not exhibit fluorescence, the organic dye Chrome azurol S (CAS) has been chosen to allow detection. The effect of metal-quenching on CAS excitation and emission conditions (λex=300nm; λem=407nm) was used as criterion for analyte quantification in presence of sodium cholate bile salt (NaC). The quenching mechanism was explored, and it can be classified as a collisional type with a Stern-Volmer constant value of 3...
September 1, 2017: Talanta
https://www.readbyqxmd.com/read/28602192/mediated-electrochemical-method-for-the-determination-of-indigo-carmine-levels-in-food-products
#3
Majid Arvand, Mehrnoosh Saberi, Masoomeh Sayyar Ardaki, Asadollah Mohammadi
In this paper, we have studied the electrochemical behavior of indigo carmine (IC) in the presence and absence of mediator molecules. We have used three azo dyes as mediators in our studies and the effect of mediator molecules on the electron transfer between the IC solution and the electrode surface was explained from the cyclic voltammograms data. Among these dyes, 4-(4-nitrophenilazo)N-benzyl,N-ethylaniline (NBE) showed good performance as an electron transfer mediator. The NBE/carbon paste was characterized with Fourier transform infrared spectroscopy and scanning electron microscopy methods...
October 1, 2017: Talanta
https://www.readbyqxmd.com/read/28601037/enterococcus-species-for-the-one-pot-biofabrication-of-gold-nanoparticles-characterization-and-nanobiotechnological-applications
#4
Iyabo Christianah Oladipo, Agbaje Lateef, Joseph Adetunji Elegbede, Musibau Adewuyi Azeez, Tesleem Babatunde Asafa, Taofeek Akangbe Yekeen, Akeem Akinboro, Evariste Bosco Gueguim-Kana, Lorika Selomi Beukes, Tolulope Oluyomi Oluyide, Oluwatoyin Rebecca Atanda
In the current work, cell-free extracts of four strains of non-pathogenic Enterococcus species of food origin, were studied for the green synthesis of gold nanoparticles (AuNPs), and characterized by UV-Vis absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The AuNPs were evaluated for their Anopheles gambiae larvicidal, dye degradation, antioxidant and thrombolytic activities. The blue-black colloidal AuNPs which absorbed maximally at 549-552nm were nearly spherical in shape, and crystalline in nature with size of 8-50nm...
June 3, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28600852/extraction-methods-and-food-uses-of-a-natural-red-colorant-from-dye-sorghum
#5
Fug Akogou, App Kayodé, Hmw den Besten, A R Linnemann
BACKGROUND: The interest in stable natural colorants for food applications continues to grow. A red pigment extracted from the leaf sheaths of a sorghum variety (Sorghum bicolor) with a high content of apigeninidin is widely used as a biocolorant in processed foods in West-Africa. This study compared the colour and the anthocyanin composition from traditional extraction methods to determine options for improvement and use of the red biocolorant from dye sorghum in the food sector. RESULTS: Sorghum biocolorant was commonly applied in fermented and heated foods...
June 10, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28597218/uncovering-a-new-moral-dilemma-of-economic-optimization-in-biotechnological-processing
#6
Marek Vochozka, Vojtěch Stehel, Anna Maroušková
The trend of emerging biorefineries is to process the harvest as efficiently as possible and without any waste. From the most valuable phytomass, refined medicines, enzymes, dyes and other special reactants are created. Functional foods, food ingredients, oils, alcohol, solvents, plastics, fillers and a wide variety of other chemical products follow. After being treated with nutrient recovery techniques (for fertilizer production), biofuels or soil improvers are produced from the leftovers. Economic optimization algorithms have confirmed that such complex biorefineries can be financially viable only when a high degree of feedstock concentration is included...
June 8, 2017: Science and Engineering Ethics
https://www.readbyqxmd.com/read/28589774/development-of-a-multiplex-qpcr-in-real-time-for-quantification-and-differential-diagnosis-of-salmonella-gallinarum-and-salmonella-pullorum
#7
Marcela da Silva Rubio, Rafael Antonio Casarin Penha Filho, Adriana Maria de Almeida, Angelo Berchieri Junior
Currently there are 2659 Salmonella serovars. The host-specific biovars Salmonella Pullorum and Salmonella Gallinarum, cause systemic infections in food-producing and wild birds. Fast diagnosis is crucial to control the dissemination in avian environments. The present work describes the development of a multiplex qPCR in real time using a low cost DNA dye (SYBr Green) to identify and quantify these biovars. Primers were chosen based on genomic regions of difference (RoD) and optimized to control dimers. Primers pSGP differentiate from other serovars and pSG and pSP differentiate biovars...
June 7, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28578045/impact-of-laccase-on-the-colour-stability-of-structured-oil-in-water-emulsions
#8
Catherine K Y Chan, Benjamin Zeeb, David Julian McClements, Jochen Weiss
The optical properties of food emulsions play a key role in determining their perceived quality because they are the first sensory cue that many consumers receive. The purpose of the current study was to investigate the impact of a cross-linking enzyme (laccase) on the appearance of structured oil-in-water emulsions containing a lipophilic model colorant (Nile red). A layer-by-layer electrostatic deposition approach was used to prepare oil-in-water emulsions stabilized by interfacial protein-pectin complexes under acidic conditions (pH3...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28572641/application-of-nuclear-magnetic-resonance-spectroscopy-in-food-adulteration-determination-the-example-of-sudan-dye-i-in-paprika-powder
#9
Yaxi Hu, Shuo Wang, Shenlin Wang, Xiaonan Lu
Carcinogenic Sudan I has been added illegally into spices for an apparent freshness. (1)H solution and solid-state (SS) nuclear magnetic resonance (NMR) spectroscopies were applied and compared for determination of Sudan I in paprika powders (PPs). For solution NMR, PPs spiked with Sudan I were extracted with acetonitrile, centrifuged, rotor-evaporated, and re-dissolved in DMSO-d6 for spectral collection. For SSNMR, Sudan I contaminated PPs were mixed with DMSO-d6 solution and used for spectral collection. Linear regression models constructed for quantitative analyses resulted in the average accuracies for unknown samples as 98% and 105%, respectively...
June 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28570907/melanin-gamma-rays-assistants-for-bismuth-oxide-nanoparticles-synthesis-at-room-temperature-for-enhancing-antimicrobial-and-photocatalytic-activity
#10
Ahmed I El-Batal, Gharieb S El-Sayyad, Abbas El-Ghamry, Kerolos M Agaypi, Mohamed A Elsayed, Mohamed Gobara
Melanin pigment has been deemed as a natural photoprotector with strong hydrophobicity. It allured considerable compatibility with many applications in medicine, food, and nanotechnology. Penicillium chrysogenium has been devoted to the green synthesis of melanin whereby optimizing its culture and environmental conditions. The impacts of alternative economic L-tyrosine natural sources (unprecedented alternate origins) and gamma radiation were pledged for the potential growing of the pigment. Herein, notable increases in melanin yield (6...
May 23, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28570582/handling-of-thermal-paper-implications-for-dermal-exposure-to-bisphenol-a-and-its-alternatives
#11
Meghan R Bernier, Laura N Vandenberg
Bisphenol A (BPA) is an endocrine disrupting chemical used in a wide range of consumer products including photoactive dyes used in thermal paper. Recent studies have shown that dermal absorption of BPA can occur when handling these papers. Yet, regulatory agencies have largely dismissed thermal paper as a major source of BPA exposure. Exposure estimates provided by agencies such as the European Food Safety Authority (EFSA) are based on assumptions about how humans interact with this material, stating that 'typical' exposures for adults involve only one handling per day for short periods of time (<1 minute), with limited exposure surfaces (three fingertips)...
2017: PloS One
https://www.readbyqxmd.com/read/28549185/visibility-and-persistence-of-marker-dyes-and-effect-on-the-quality-and-mating-competitiveness-of-mass-reared-flies-diptera-tephritidae-anastrepha-obliqua-and-bisexual-and-genetic-sexing-tapachula-7-strains-of-a-ludens
#12
José Arredondo, Lia Ruiz, Gladis López, Francisco Díaz-Fleischer
Fluorescent dyes are commonly used in the sterile insect technique (SIT) for marking insects for a proper identification after recapture. However, the quality of the mark must be balanced against insect performance, because dyes can negatively affect some parameters of insect performance and reduce their effectiveness in control with the SIT. We determined the visibility and persistence and the effect of dyes on the quality of Anastrepha obliqua (Macquart) and Anastrepha ludens (Loew) (bisexual and genetic sexing strains) by testing four concentrations of a dye (Day-Glo) from 0 to 2...
May 26, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28528254/quantitative-determination-of-auramine-o-by-terahertz-spectroscopy-with-2dcos-plsr%C3%A2-model
#13
Huo Zhang, Zhi Li, Tao Chen, Binyi Qin
Residues of harmful dyes such as Auramine O (AO) in herb and food products threaten the health of people. So, fast and sensitive detection techniques of the residues are needed. As a powerful tool for substance detection, terahertz (THz) spectroscopy was used for the quantitative determination of AO by combining with an improved partial least-squares regression (PLSR) model in this paper. Absorbance of herbal samples with different concentrations was obtained by THz-TDS in the band between 0.2THz and 1.6THz...
May 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28524270/interaction-studies-of-copper-complex-containing-food-additive-carmoisine-dye-with-human-serum-albumin-hsa-spectroscopic-investigations
#14
Nahid Shahabadi, Alireza Akbari, Mina Jamshidbeigi, Soraya Moradi Fili
In this study, the interaction between human serum albumin (HSA) and a copper complex of carmoisine dye; [Cu(carmoisine)2 (H2 O)2 ], was studied in vitro using multi-spectroscopic methods. It was found that the intrinsic fluorescence of HSA was quenched by the addition of the [Cu(carmoisine)2 (H2 O)2 ] complex and the quenching mechanism was considered as static quenching by formation of a [Cu(carmoisine)2 (H2 O)2 ]-HSA complex. The binding constant was about 10(4)  M(-1) at room temperature. The values of the calculated thermodynamic parameters (ΔH < 0 and ΔS > 0) suggested that both hydrogen bonds and the hydrophobic interactions were involved in the binding process...
May 19, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28516787/antiproliferative-effect-of-a-food-coloring-on-colon-cancer-cell-line
#15
M Norizadeh Tazehkand
4-MEI (4-Methylimidazole) is used as a chemical intermediate, crude material or component in the manufacture of pharmaceuticals, photographic and photothermographic chemicals, dyes and pigments and agricultural chemicals. 4-MEI is unintentionally found in our food. Caramel colour (which is the most used beverage colouring and food), dark beers and common brands of cola drinks may comprise more than 100 μg of this compound per 12-ounce serving. 4-MEI is widely used by people and colon cancer is common in our countries...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/28506084/turmeric-old-spice-new-spice
#16
C J Cooksey
The history of chemical investigations into the yellow components of turmeric can be traced from 1815. Although the major yellow component of turmeric, curcumin, often is represented as a 1,3-diketone in the solid state and in nonaqueous solution, it exists in the enol form. The struggle to identify the chemical structure of curcumin continued for nearly a century and was complicated by the difficult purification of curcumin and by the presence of two additional yellow components. Food remains the main use for turmeric (old spice); its use as a dye has diminished since the 19th century, but it may have pharmaceutical uses (new spice)...
May 16, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28501199/developing-a-sensor-layer-for-the-optical-detection-of-amines-during-food-spoilage
#17
Cindy Schaude, Claudia Meindl, Eleonore Fröhlich, Jennifer Attard, Gerhard J Mohr
A colourimetric sensor layer has been developed for ammonia and biogenic amines. Amine exposure induces a traffic light colour change from green to red. Recognition is performed by a pH indicator dye, covalently immobilised onto cellulose microparticles. The sensor microparticles are embedded into food-grade silicone. Selectivity of the pH indicator dye towards gaseous amine is obtained by complete embedding of the sensor particles within the ion-impermeable silicone. A response time of 1.5h has been achieved, with a reverse response occurring after 20h...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28490150/a-simple-and-sensitive-vortex-assisted-ionic-liquid-dispersive-microextraction-and-spectrophotometric-determination-of-selenium-in-food-samples
#18
Esra Bağda, Mustafa Tüzen
In the present study, a novel and eco-friendly vortex-assisted ionic liquid-based microextraction method was developed for the determination of selenium in food. The microextraction method is based on the liberation of iodine in the presence of selenium; the liberated iodine reacts with I(-) to form I3(-). Anionic I3(-) reacts with cationic crystal violet dye, and the product is extracted into 1-hexyl-3-methylimidazolium hexafluorophosphate phase in the presence of Triton X-114. The proposed method is linear in the range of 2...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28486808/limited-tyrosine-utilization-explains-lower-betalain-contents-in-yellow-than-in-red-table-beet-genotypes
#19
Minmin Wang, Samuel Lopez-Nieves, Irwin L Goldman, Hiroshi A Maeda
Betalains are tyrosine-derived pigments that consist of red-violet betacyanins and yellow betaxanthins. These pigments are major sources of natural food dyes in the United States. Decades of table beet breeding efforts have increased betalain pigmentation, but yellow betaxanthin accumulation has been lower than that of red betacyanins. To identify possible bottlenecks in betalain production, here we conducted comparative analyses of betalains and their precursor, tyrosine, in various beet genotypes. Consistent with previous studies, red beets had much higher betalain concentrations than yellow beets...
May 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28462057/membrane-permeabilization-of-mammalian-cells-using-bursts-of-high-magnetic-field-pulses
#20
Vitalij Novickij, Janja Dermol, Audrius Grainys, Matej Kranjc, Damijan Miklavčič
BACKGROUND: Cell membrane permeabilization by pulsed electromagnetic fields (PEMF) is a novel contactless method which results in effects similar to conventional electroporation. The non-invasiveness of the methodology, independence from the biological object homogeneity and electrical conductance introduce high flexibility and potential applicability of the PEMF in biomedicine, food processing, and biotechnology. The inferior effectiveness of the PEMF permeabilization compared to standard electroporation and the lack of clear description of the induced transmembrane transport are currently of major concern...
2017: PeerJ
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