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Andrea Pio-Abreu, Luciano F Drager
PURPOSE OF REVIEW: Resistant hypertension (RH) is a growing clinical condition worldwide associated with target-organ damage and poor prognosis compared to non-resistant counterparts. The purpose of this review is to perform a critical evaluation of preferable drug choices for managing RH highlighting the evidence that significant proportion of patients remained uncontrolled despite using four anti-hypertensive drugs. RECENT FINDINGS: Until recently, the fourth drug therapy was main derived from personal opinion or small interventional studies...
June 16, 2018: Current Hypertension Reports
Jianhui Wu, Shuo Huang, Qingdong Zeng, Shengjie Liu, Qilin Wang, Jingmei Mu, Shizhou Yu, Dejun Han, Zhensheng Kang
A major stripe rust resistance QTL on chromosome 4BL was localized to a 4.5-Mb interval using comparative QTL mapping methods and validated in 276 wheat genotypes by haplotype analysis. CYMMIT-derived wheat line P10103 was previously identified to have adult plant resistance (APR) to stripe rust in the greenhouse and field. The conventional approach for QTL mapping in common wheat is laborious. Here, we performed QTL detection of APR using a combination of genome-wide scanning and extreme pool-genotyping. SNP-based genetic maps were constructed using the Wheat55 K SNP array to genotype a recombinant inbred line (RIL) population derived from the cross Mingxian 169 × P10103...
June 16, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Hitoshi Ishii, Koki Shin, Takahiro Tosaki, Tatsuya Haga, Yoshiki Nakajima, Toshihiko Shiraiwa, Nobuaki Watanabe, Miyuki Koizumi, Hiroki Nakajima, Sadanori Okada, Tsuyoshi Mashitani, Takako Mohri, Yasuhiro Akai
In the original publication, second author's name was incorrectly published as Hiroki Shin. The correct name should read as 'Koki Shin'.
June 16, 2018: Diabetes Therapy: Research, Treatment and Education of Diabetes and related Disorders
Melissa S Standley, Samuel Million-Weaver, David L Alexander, Shuai Hu, Manel Camps
ColE1-like plasmid vectors are widely used for expression of recombinant genes in E. coli. For these vectors, segregation of individual plasmids into daughter cells during cell division appears to be random, making them susceptible to loss over time when no mechanisms ensuring their maintenance are present. Here we use the plasmid pGFPuv in a recA relA strain as a sensitized model to study factors affecting plasmid stability in the context of recombinant gene expression. We find that in this model, plasmid stability can be restored by two types of genetic modifications to the plasmid origin of replication (ori) sequence: point mutations and a novel 269 nt duplication at the 5' end of the plasmid ori, which we named DAS (duplicated anti-sense) ori...
June 16, 2018: Current Genetics
Jaime Madrigal-González, Enrique Andivia, Miguel A Zavala, Markus Stoffel, Joaquín Calatayud, Raúl Sánchez-Salguero, Juan Ballesteros-Cánovas
Climate change can impair ecosystem functions and services in extensive dry forests worldwide. However, attribution of climate change impacts on tree growth and forest productivity is challenging due to multiple inter-annual patterns of climatic variability associated with atmospheric and oceanic circulations. Moreover, growth responses to rising atmospheric CO2 , namely carbon fertilization, as well as size ontogenetic changes can obscure the climate change signature as well. Here we apply Structural Equation Models (SEM) to investigate the relative role of climate change on tree growth in an extreme Mediterranean environment (i...
June 14, 2018: Science of the Total Environment
Robert Fuchs, Anika Stracke, Viktoria Holzmann, Gerfried Luschin-Ebengreuth, Nathalie Meier-Allard, Nadine Ebner, Teresa Maria Lassacher, Markus Absenger-Novak, Eleonore Fröhlich, Matthias Schittmayer, Sara Cano Crespo, Manuel Palacin, Beate Rinner, Ruth Birner-Gruenberger
The quinazoline based drug prazosin (PRZ) is a potent inducer of apoptosis in human cancer cells. We recently reported that PRZ enters cells via endocytosis and induces tubulation of the endolysosomal system. In a proteomics approach aimed at identifying potential membrane proteins with binding affinity to quinazolines, we detected the oncoprotein CD98hc. We confirmed shuttling of CD98hc towards lysosomes and upregulation of CD98hc expression in PRZ treated cells. Gene knockout (KO) experiments revealed that endocytosis of PRZ still occurs in the absence of CD98hc - suggesting that PRZ does not enter the cell via CD98hc but misroutes the protein towards tubular lysosomes...
June 14, 2018: Biochimica et Biophysica Acta
Naveen K Singh, Sunil K Arya, Pedro Estrela, Pranab Goswami
A capacitive aptasensor for detecting the malaria biomarker, Plasmodium falciparum glutamate dehydrogenase (PfGDH), directly in human serum samples developed. A thiolated ssDNA aptamer (NG3) that binds specifically to PfGDH antigen with high affinity (Kd = 79 nM) was used to develop the aptasensor. The aptasensor produced capacitance response at an optimized frequency of 2 Hz in a non-Faradaic electrochemical impedance based signal transduction platform. The aptasensor exhibited a wide dynamic range of 100 fM-100 nM with a limits of detection of 0...
June 8, 2018: Biosensors & Bioelectronics
Paul Baas, Sjaak Burgers
No abstract text is available yet for this article.
June 13, 2018: Lancet Oncology
Ulrike Blaschke, Beneditta Suwono, Sachli Zafari, Ingo Ebersberger, Evelyn Skiebe, Cy M Jeffries, Dmitri I Svergun, Gottfried Wilharm
Acinetobacter baumannii appears as an often multidrug-resistant nosocomial pathogen in hospitals worldwide. Its remarkable persistence in the hospital environment is probably due to intrinsic and acquired resistance to disinfectants and antibiotics, tolerance to desiccation stress, capability to form biofilms, and is possibly facilitated by surface-associated motility. Our attempts to elucidate surface-associated motility in A. baumannii revealed a mutant inactivated in a putative DNA-(adenine N6)-methyltransferase, designated A1S_0222 in strain ATCC 17978...
June 14, 2018: Protein Expression and Purification
Celine Gys, Ana Kovačič, Carolin Huber, Foon Yin Lai, Ester Heath, Adrian Covaci
Bisphenol S (BPS) is increasingly used as substitute for bisphenol A, resulting in higher potential of human exposure to this compound. Yet, information on the human metabolism of BPS is limited. Hence, current biomonitoring studies rely only on the measurement of BPS itself, leading to a potential underestimation of assessing human exposure to this emerging contaminant. The aims of this study were to investigate the in vitro metabolic pathways of BPS using human liver microsomes and cytosol fractions and propose in vitro metabolites for evaluation in pharmacokinetics studies...
June 14, 2018: Toxicology Letters
Radmila Lyubarova, Joshua Schulman-Marcus
No abstract text is available yet for this article.
June 5, 2018: Trends in Cardiovascular Medicine
T Xu, A S M Alharthi, F Batistel, A Helmbrecht, C Parys, E Trevisi, X Shen, J J Loor
The study investigated whether methionine supply during late pregnancy is associated with liver mammalian target of rapamycin (MTOR) pathway phosphorylation, plasma biomarkers, and growth in heifer calves born to cows fed a control diet (CON) or the control diet plus ethylcellulose rumen-protected methionine (MET; 0.09% of dry matter intake) for the last 28 d prepartum. Calves were fed and managed similarly during the first 56 d of age. Plasma was harvested at birth and 2, 7, 21, 42, and 50 d of age and was used for biomarker profiling...
June 13, 2018: Journal of Dairy Science
(no author information available yet)
Posthumous gamete (sperm or oocyte) retrieval or use for reproductive purposes is ethically justifiable if written documentation from the deceased authorizing the procedure is available. Retrieval of sperm or eggs does not commit a center to their later use for reproduction, but may be permissible under the circumstances outlined in this opinion. Embryo use is also justifiable with such documentation. In the absence of written documentation from the decedent, programs open to considering requests for posthumous use of embryos or gametes should only do so when such requests are initiated by the surviving spouse or partner...
June 13, 2018: Fertility and Sterility
Yingjie Li, Wenfeng Li, Qingnan Liu, Hong Meng, Yingzhou Lu, Chunxi Li
For the first time, a series of alkynyl carbon materials (ACMs) were prepared via the mechanochemical reaction of CaC2 with six polyhalogenated precursors, namely CCl4 , C2 Cl6 , C2 Cl4 , C6 Cl6 , C6 Br6 , and C14 H4 Br10 (ACM-1, ACM-2, ACM-3, ACM-4, ACM-5, and ACM-6, respectively) and used for the adsorptive removal of mercury from aqueous solutions. Based on preliminary investigations, the adsorption of mercury on ACM-5 was studied in depth. Specifically, the effect of pH on mercury adsorptivity, adsorption kinetics, thermodynamics, isotherms, and recyclability was studied...
June 2018: Journal of Environmental Sciences (China)
K Cizkova, Z Tauber
CYP2C and CYP2 J enzymes, commonly named as cytochrome P450 (CYP) epoxygenases, convert arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EETs), biologically active eicosanoids with many functions in organism. EETs are rapidly hydrolysed to less active dihydroxyeicosatrienoic acids (DHETs) by soluble epoxide hydrolase (sEH). We investigated spatio-temporal expression pattern of CYP2C8, CYP2C9, CYP2 J2 and sEH in normal human placenta by immunohistochemical method. In the villous trophoblast, CYP2C8 was the most abundant protein...
June 13, 2018: Acta Histochemica
Sevgi Polat, Perviz Sayan
The polymorphic phase transformation of β-glycine to α-glycine was analyzed in the absence and presence of three different intensities of ultrasonic irradiation in a batch system at 25 °C. The experiments were performed to explore the effects of ultrasonic irradiation and its intensity on phase transformation time, morphology, storage stability, and filtration characteristics. The crystals obtained with and without ultrasound were characterized by X-ray diffraction (XRD). The XRD results showed that the ultrasound tended to reduce the transformation time...
October 2018: Ultrasonics Sonochemistry
Claudio Conforti, Emanuela Beninanti, Caterina Dianzani
Actinic keratosis (AK) is a very common skin disease caused by chronic sun exposure. AKs have historically been characterized as being "precancerous" or "premalignant." It is true that these lesions do not possess metastatic potential, because they are confined to the epidermis, but it is not accurate to deem them "premalignant." AK qualifies as a malignant neoplasm, because it also fulfills criteria for malignancy in classic pathology, namely, the capability, or potential, to kill by either destruction of tissue locally or by metastasis widely...
May 2018: Clinics in Dermatology
Hossein Akbari, Mostafa Ghardashi, Alireza Soleimani, Hanieh Mohammadi, Hassan Nikoueinejad
A subset of CD4+ T cells named T helper (Th)22 cells play some pathogenic roles in some autoimmune disorders such as type 1 diabetes (T1D). We aimed to study the correlation between the circulatory number of these cells and serum levels of its related cytokines with T1D as well as diabetic complications including metabolic control, atherosclerosis, and nephropathy. Fortynine patients with T1D and 43 healthy controls underwent the assessment of circulatory number of Th22 cells (by flow cytometry), serum level of Th22 related cytokines including Interleukin-22 (IL-22), Interleukin-10 (IL-10), Transforming growth factor-β (TGF-β), Tumor necrosis factor-α (TNF-α) (by ELISA) and carotid intima-media thickness (cIMT) measurement (by doppler ultrasonography)...
June 2018: Iranian Journal of Allergy, Asthma, and Immunology
Bei Wen, Ying Pan, Xiaoli Shi, Hongna Zhang, Xiaoyu Hu, Honglin Huang, Jitao Lv, Shuzhen Zhang
Perfluorooctane sulfonate (PFOS) precursors have been found extensively in sewage sludge and biosolids-amended soils. The degradation of these precursors are regarded as a significant source of PFOS in the environment. In this study, the accumulation of N-ethyl perfluorooctane sulfonamido acetic acid (N-EtFOSAA) in the plants of seven species, namely alfalfa, lettuce, maize, mung bean, radish, ryegrass, and soybean from biosolids-amended soil, and the degradation kinetics of N-EtFOSAA in soil-plant microcosms were evaluated over 60 days...
June 13, 2018: Science of the Total Environment
Quan H Le, Peter J T Verheijen, Mark C M van Loosdrecht, Eveline I P Volcke
A stepwise experimental design procedure to obtain reliable data from wastewater treatment plants (WWTPs) was developed. The proposed procedure aims at determining sets of additional measurements (besides available ones) that guarantee the identifiability of key process variables, which means that their value can be calculated from other, measured variables, based on available constraints in the form of linear mass balances. Among all solutions, i.e. all possible sets of additional measurements allowing the identifiability of all key process variables, the optimal solutions were found taking into account two objectives, namely the accuracy of the identified key variables and the cost of additional measurements...
May 15, 2018: Water Research
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