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streptococcus mitis+pneumonia

Anders Jensen, Christian F P Scholz, Mogens Kilian
The Mitis group of the genus Streptococcus currently consists of 20 formally describes species, including the pathogen S. pneumoniae. They have been the subject of much taxonomic confusion, due to phenotypic overlap and genetic heterogeneity, which has hampered the full appreciation of their clinical significance. The purpose of this study was to critically re-examine the taxonomy of the Mitis group using 195 publicly available genomes including designated type strains for phylogenetic analyses based on core genomes, multilocus sequences (MLSA), and 16S rRNA gene sequences combined with estimates of average nucleotide identity (ANI) and in silico and in vitro analyses of specific phenotypic characteristics...
August 17, 2016: International Journal of Systematic and Evolutionary Microbiology
Blas Blázquez, Alba Fresco-Taboada, Manuel Iglesias-Bexiga, Margarita Menéndez, Pedro García
The emergence and spread of antibiotic-resistant bacteria is pushing the need of alternative treatments. In this context, phage therapy is already a reality to successfully fight certain multiresistant bacteria. Among different phage gene products, murein hydrolases responsible of phage progeny liberation (also called lysins or endolysins) are weapons that target specific peptidoglycan bonds, leading to lysis and death of susceptible bacteria when added from the outside. In the pneumococcal system, all but one phage murein hydrolases reported to date share a choline-binding domain that recognizes cell walls containing choline residues in the (lipo)teichoic acids...
2016: Frontiers in Microbiology
Jun Hirai, Daisuke Sakanashi, Mao Hagihara, Shusaku Haranaga, Kohei Uechi, Hideo Kato, Hiroyuki Hamada, Naoya Nishiyama, Yusuke Koizumi, Hiroyuki Suematsu, Yuka Yamagishi, Jiro Fujita, Hiroshige Mikamo
Gene sequence analysis methods, including 16S rRNA identification, allows accurate identification of Streptococcus species, which include phenotypically closely related species that are difficult to differentiate using conventional chemical methods. We report a case of bacteremia due to Streptococcus tigurinus, identified by 16S rRNA, in a 72-year-old woman with gastrointestinal cancer and ascites. She was hospitalized to undergo elective tumor-related surgery. Five days prior to undergoing surgery, she developed a fever with no obvious source of infection...
July 23, 2016: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
K E B van Veen, M C Brouwer, A van der Ende, D van de Beek
We performed a nationwide prospective cohort study on the epidemiology and clinical features of community-acquired bacterial meningitis. Patients with a medical history of autologous or allogeneic hematopoietic stem cell transplantation (HSCT) were identified from the cohort performed from March 2006 to October 2014. Fourteen of 1449 episodes (1.0%) of bacterial meningitis occurred in patients with a history of HSCT. The incidence of bacterial meningitis in HSCT recipients was 40.4 per 100 000 patients per year (95% confidence interval (CI) 23...
July 4, 2016: Bone Marrow Transplantation
Irene Burckhardt, Jessica Panitz, Mark van der Linden, Stefan Zimmermann
Streptococcus pneumoniae is a rare cause of urinary tract infection. Between January 2010 and December 2014, 26 urine samples from 18 different patients contained S. pneumoniae at the Department for Infectious Diseases, University Hospital of Heidelberg. Patient age varied between three and 72 years. 13 patients were male and five were female. Past medical histories of 16 patients were available. Eight patients had a past medical history of renal transplant and four patients had other renal dysfunctions. Further analyses of the isolates revealed that the aspect of colonies is more resembling S...
September 2016: Diagnostic Microbiology and Infectious Disease
Dalia Denapaite, Martin Rieger, Sophie Köndgen, Reinhold Brückner, Irma Ochigava, Peter Kappeler, Kerstin Mätz-Rensing, Fabian Leendertz, Regine Hakenbeck
Viridans streptococci were obtained from primates (great apes, rhesus monkeys, and ring-tailed lemurs) held in captivity, as well as from free-living animals (chimpanzees and lemurs) for whom contact with humans is highly restricted. Isolates represented a variety of viridans streptococci, including unknown species. Streptococcus oralis was frequently isolated from samples from great apes. Genotypic methods revealed that most of the strains clustered on separate lineages outside the main cluster of human S...
March 2016: MSphere
P Seng, M Vernier, A Gay, P-O Pinelli, R Legré, A Stein
Streptococcal bone and joint infections are less common than staphylococcal cases. Few studies have reported the cases with well-identified Streptococcus species. Their clinical features and prognosis are not clearly known to date. Moreover, no treatment regimen has yet been clarified. We reviewed the streptococcal bone and joint infection cases managed in our centres from January 2009 to December 2013. We described the epidemiology, clinical and microbiologic characteristics, treatment approach and outcome...
July 2016: New Microbes and New Infections
Alexandra S Simões, Débora A Tavares, Dora Rolo, Carmen Ardanuy, Herman Goossens, Birgitta Henriques-Normark, Josefina Linares, Hermínia de Lencastre, Raquel Sá-Leão
During surveillance studies we detected, among over 1500 presumptive pneumococci, 11 isolates displaying conflicting or novel results when characterized by widely accepted phenotypic (optochin susceptibility and bile solubility) and genotypic (lytA-BsaAI-RFLP and MLST) identification methods. We aimed to determine the genetic basis for the unexpected results given by lytA-BsaAI-RFLP and investigate the accuracy of the WHO recommended lytA real-time PCR assay to classify these 11 isolates. Three novel lytA-BsaAI-RFLP signatures were found (one in pneumococcus and two in S...
June 2016: Diagnostic Microbiology and Infectious Disease
Joffrey van Prehn, Suzanne Q van Veen, Jacqueline J G Schelfaut, Els Wessels
We compared the Vitek MS and Microflex MALDI-TOF mass spectrometry platform for species differentiation within the Streptococcus mitis group with PCR assays targeted at lytA, Spn9802, and recA as reference standard. The Vitek MS correctly identified 10/11 Streptococcus pneumoniae, 13/13 Streptococcus pseudopneumoniae, and 12/13 S. mitis/oralis. The Microflex correctly identified 9/11 S. pneumoniae, 0/13 S. pseudopneumoniae, and 13/13 S. mitis/oralis. MALDI-TOF is a powerful tool for species determination within the mitis group...
May 2016: Diagnostic Microbiology and Infectious Disease
Zhengli Wang, Qingqing Xiang, Ting Yang, Luquan Li, Jingli Yang, Hongong Li, Yu He, Yunhui Zhang, Qi Lu, Jialin Yu
Streptococcus mitis (S. mitis) and Pseudomonas aeruginosa (P. aeruginosa) are typically found in the upper respiratory tract of infants. We previously found that P. aeruginosa and S. mitis were two of the most common bacteria in biofilms on newborns' endotracheal tubes (ETTs) and in their sputa and that S. mitis was able to produce autoinducer-2 (AI-2), whereas P. aeruginosa was not. Recently, we also found that exogenous AI-2 and S. mitis could influence the behaviors of P. aeruginosa. We hypothesized that S...
2016: Frontiers in Microbiology
Natalie Maricic, Erica S Anderson, AnneMarie E Opipari, Emily A Yu, Suzanne Dawid
UNLABELLED: Bacterial communities are established through a combination of cooperative and antagonistic interactions between the inhabitants. Competitive interactions often involve the production of antimicrobial substances, including bacteriocins, which are small antimicrobial peptides that target other community members. Despite the nearly ubiquitous presence of bacteriocin-encoding loci, inhibitory activity has been attributed to only a small fraction of gene clusters. In this study, we characterized a novel locus (the pld locus) in the pathogen Streptococcus pneumoniae that drives the production of a bacteriocin called pneumolancidin, which has broad antimicrobial activity...
2016: MBio
Nicolas Gisch, Dominik Schwudke, Simone Thomsen, Nathalie Heß, Regine Hakenbeck, Dalia Denapaite
Members of the Mitis group of streptococci possess teichoic acids (TAs) as integral components of their cell wall that are unique among Gram-positive bacteria. Both, lipoteichoic (LTA) and wall teichoic acid, are formed by the same biosynthetic pathway, are of high complexity and contain phosphorylcholine (P-Cho) residues. These residues serve as anchors for choline-binding proteins (CBPs), some of which have been identified as virulence factors of the human pathogen Streptococcus pneumoniae. We investigated the LTA structure of its close relative Streptococcus oralis...
2015: Scientific Reports
Ma Fátima López-Fabal, José Luís Gómez-Garcés, José Luís López-Hontangas, Nuria Sanz, Carmen Muñoz, Marta Regodón
Mass spectrometry has become a reference resource for identifying microorganisms in clinical microbiology services. One hundred and fifty one clinical isolates were selected from respiratory specimens routinely identified as Streptococcus pneumoniae (43), Haemophilus influenzae (64) and Moraxella catarrhalis (44). These identifications were compared with other phenotypical methods and mass spectrometry (MALDI-TOF-MS Vitek). Result discrepancies were assessed by 16S rRNA sequencing. Thirty-eight of the 43 strains of S...
October 2015: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
Mihai Pop, Alan W Walker, Joseph Paulson, Brianna Lindsay, Martin Antonio, M Anowar Hossain, Joseph Oundo, Boubou Tamboura, Volker Mai, Irina Astrovskaya, Hector Corrada Bravo, Richard Rance, Mark Stares, Myron M Levine, Sandra Panchalingam, Karen Kotloff, Usman N Ikumapayi, Chinelo Ebruke, Mitchell Adeyemi, Dilruba Ahmed, Firoz Ahmed, Meer Taifur Alam, Ruhul Amin, Sabbir Siddiqui, John B Ochieng, Emmanuel Ouma, Jane Juma, Euince Mailu, Richard Omore, J Glenn Morris, Robert F Breiman, Debasish Saha, Julian Parkhill, James P Nataro, O Colin Stine
BACKGROUND: Diarrheal diseases continue to contribute significantly to morbidity and mortality in infants and young children in developing countries. There is an urgent need to better understand the contributions of novel, potentially uncultured, diarrheal pathogens to severe diarrheal disease, as well as distortions in normal gut microbiota composition that might facilitate severe disease. RESULTS: We use high throughput 16S rRNA gene sequencing to compare fecal microbiota composition in children under five years of age who have been diagnosed with moderate to severe diarrhea (MSD) with the microbiota from diarrhea-free controls...
2014: Genome Biology
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