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https://www.readbyqxmd.com/read/28209515/cell-type-specific-role-of-the-rna-binding-protein-nono-in-the-dna-double-strand-break-response-in-the-mouse-testes
#1
Shuyi Li, Feng-Jue Shu, Zhentian Li, Lahcen Jaafar, Shourong Zhao, William S Dynan
The tandem RNA recognition motif protein, NONO, was previously identified as a candidate DNA double-strand break (DSB) repair factor in a biochemical screen for proteins with end-joining stimulatory activity. Subsequent work showed that NONO and its binding partner, SFPQ, have many of the properties expected for bona fide repair factors in cell-based assays. Their contribution to the DNA damage response in intact tissue in vivo has not, however, been demonstrated. Here we compare DNA damage sensitivity in the testes of wild-type mice versus mice bearing a null allele of the NONO homologue (Nono (gt))...
February 10, 2017: DNA Repair
https://www.readbyqxmd.com/read/28208955/high-serum-estradiol-and-heavy-metals-responsible-for-human-spermiation-defect-a-pilot-study
#2
Manish Jain, Amanpreet Kaur Kalsi, Amita Srivastava, Yogendra Kumar Gupta, Ashutosh Halder
INTRODUCTION: Spermiation is a process of releasing sperm into the lumen of seminiferous tubules. Failure in releasing sperm into the lumen is designated as spermiation defect. Spermiation defect cases present as oligo-azoospermia or azoospermia despite normal gonadotropins and testicular histology/cytology. Human spermiation defect never got attention to investigate infertility practice. Most of the information on spermiation defect, so far is from animal experiments. We assume some cases of non-obstructive azoospermia with normal gonadotropins and testicular histology/cytology could be due to spermiation defect...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28208702/the-glutamate-dehydrogenase-pathway-and-its-roles-in-cell-and-tissue-biology-in-health-and-disease
#3
REVIEW
Andreas Plaitakis, Ester Kalef-Ezra, Dimitra Kotzamani, Ioannis Zaganas, Cleanthe Spanaki
Glutamate dehydrogenase (GDH) is a hexameric enzyme that catalyzes the reversible conversion of glutamate to α-ketoglutarate and ammonia while reducing NAD(P)⁺ to NAD(P)H. It is found in all living organisms serving both catabolic and anabolic reactions. In mammalian tissues, oxidative deamination of glutamate via GDH generates α-ketoglutarate, which is metabolized by the Krebs cycle, leading to the synthesis of ATP. In addition, the GDH pathway is linked to diverse cellular processes, including ammonia metabolism, acid-base equilibrium, redox homeostasis (via formation of fumarate), lipid biosynthesis (via oxidative generation of citrate), and lactate production...
February 8, 2017: Biology
https://www.readbyqxmd.com/read/28207192/temporal-spatial-establishment-of-initial-niche-for-the-primary-spermatogonial-stem-cell-formation-is-determined-by-an-arid4b-regulatory-network
#4
Ray-Chang Wu, Yang Zeng, Yu-Fang Chen, Rainer B Lanz, Mei-Yi Wu
During neonatal testis development, centrally located gonocytes migrate to basement membrane of the seminiferous cords, where physical contact with a niche established by Sertoli cells is essential for transition of gonocytes into spermatogonial stem cells (SSCs). To provide structural support and signaling stimuli for the gonocyte-to-SSC transition that occurs at a specific location during a finite phase, temporal-spatial establishment of the niche is critical. To date, the factors that guide Sertoli cells to establish the initial stem cell niche remain largely unknown...
February 16, 2017: Stem Cells
https://www.readbyqxmd.com/read/28204871/the-role-of-beta-catenin-mutation-and-sox9-expression-in-sex-cord-stromal-tumours-of-the-testis
#5
F Bremmer, C L Behnes, H U Schildhaus, N T Gaisa, H Reis, H Jarry, H J Radzun, P Stroebel, S Schweyer
The WHO classification of testis tumours includes the group of sex cord-stromal tumours. They are divided into several histological types, i.e. Leydig cell (LCT) and Sertoli cell tumours (SCT). Based on the physiological expression of β-catenin in normal testis/Sertoli cells, it was previously shown that SCT can carry a β-catenin mutation, causing a nuclear positivity for β-catenin and cyclin D1. Furthermore, it could be shown that the stabilization of β-catenin in Sertoli cells causes the loss of the Sertoli cell marker SOX9...
February 16, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28203706/the-ubiquitous-isoform-of-na-k-atpase-atp1a1-regulates-junctional-proteins-connexin-43-and-claudin-11-via-src-egfr-erk1-2-creb-pathway-in-rat-sertoli-cells%C3%A2
#6
Gayathri D Rajamanickam, John P Kastelic, Jacob C Thundathil
No abstract text is available yet for this article.
February 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28202061/in-vitro-generation-of-sertoli-like-and-haploid-spermatid-like-cells-from-human-umbilical-cord-perivascular-cells
#7
Ekaterina Shlush, Leila Maghen, Sonja Swanson, Shlomit Kenigsberg, Sergey Moskovtsev, Tanya Barretto, Andrée Gauthier-Fisher, Clifford L Librach
BACKGROUND: First trimester (FTM) and term human umbilical cord-derived perivascular cells (HUCPVCs), which are rich sources of mesenchymal stem cells (MSCs), can give rise to Sertoli cell (SC)-like as well as haploid germ cell (GC)-like cells in vitro using culture conditions that recapitulate the testicular niche. Gamete-like cells have been produced ex vivo using pluripotent stem cells as well as MSCs. However, the production of functional gametes from human stem cells has yet to be achieved...
February 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28196369/leveraging-online-resources-to-prioritize-candidate-genes-for-functional-analyses-using-the-fetal-testis-as-a-test-case
#8
Kathryn S McClelland, Humphrey H-C Yao
With each new microarray or RNA-seq experiment, massive quantities of transcriptomic information are generated with the purpose to produce a list of candidate genes for functional analyses. Yet an effective strategy remains elusive to prioritize the genes on these candidate lists. In this review, we outline a prioritizing strategy by taking a step back from the bench and leveraging the rich range of public databases. This in silico approach provides an economical, less biased, and more effective solution. We discuss the publicly available online resources that can be used to answer a range of questions about a gene...
February 15, 2017: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
https://www.readbyqxmd.com/read/28192519/arachidonic-acid-triggers-ca2-i-increases-in-rat-round-spermatids-by-a-likely-gpr-activation-erk-signalling-and-er-acidic-compartments-ca2-release
#9
Joaquin Paillamanque, Ana Sanchez-Tusie, Emerson M Carmona, Claudia L Treviño, Carolina Sandoval, Francisco Nualart, Nelson Osses, Juan G Reyes
Arachidonic acid (AA), a compound secreted by Sertoli cells (SC) in a FSH-dependent manner, is able to induce the release of Ca2+ from internal stores in round spermatids and pachytene spermatocytes. In this study, the possible site(s) of action of AA in round spermatids, the signalling pathways associated and the intracellular Ca2+ stores targeted by AA-induced signalling were pharmacologically characterized by measuring intracellular Ca2+ using fluorescent Ca2+ probes. Our results suggest that AA acts by interacting with a fatty acid G protein coupled receptor, initiating a G protein signalling cascade that may involve PLA2 and ERK activation, which in turn opens intracellular ryanodine-sensitive channels as well as NAADP-sensitive channels in acidic intracellular Ca2+ stores...
2017: PloS One
https://www.readbyqxmd.com/read/28192182/male-reproductive-toxicity-of-crvi-in-utero-exposure-to-crvi-at-the-critical-window-of-testis-differentiation-represses-the-expression-of-sertoli-cell-tight-junction-proteins-and-hormone-receptors-in-adult-f1-progeny-rats
#10
Kathiresh M Kumar, Mariajoseph Michael Aruldhas, Sheerin L Banu, Balaji Sadasivam, Ganapathy Vengatesh, Karthik M Ganesh, Shobana Navaneethabalakrishnan, Ajith Kumar Navin, Felicia Mary Michael, Sankar Venkatachalam, Jone A Stanley, Ilangovan Ramachandran, Sakhila K Banu, Mohammad Abdulkader Akbarsha
The effect of gestational exposure to CrVI (occupational/environmental pollutant and target to Sertoli cells(SC)) was tested in a rat model during the testicular differentiation from the bipotential gonad may interrupt spermatogenesis by disrupting SC tight junctions(TJ) and it's proteins and hormone receptors. Pregnant Wistar rats were exposed to 50/100/200ppm CrVI through drinking water during embryonic days 9-14. On Postnatal day 120, testes were subjected to ion exchange chromatographic analysis revealed increased level of CrIII in SCs and germ cells, serum and testicular interstitial fluid(TIF)...
February 9, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28190795/progress-in-understanding-the-molecular-functions-of-ddx3y-dby-in-male-germ-cell-development-and-maintenance
#11
Alexei A Kotov, Oxana M Olenkina, Baira K Godneeva, Vladimir E Adashev, Ludmila V Olenina
Human DDX3 paralogs are housed on the X chromosome (DDX3X) as well as in the non- recombining region Yq11 of the Y-chromosome (DDX3Y or DBY). A gene encoding RNA helicase DDX3Y is located in the AZoospermia Factor a (AZFa) region of the Y-chromosome and expressed only in male germ cells. Deletions encompassing the DDX3Y gene lead to azoospermia and cause Sertoli Cell-Only Syndrome (SCOS) in humans. SCOS is characterized by a complete germ cell lack with preservation of somatic Sertoli cells. This review summarizes current advances in the study of DDX3Y functions in maintenance and development of early male germ cells...
February 12, 2017: Bioscience Trends
https://www.readbyqxmd.com/read/28188686/toxic-effects-of-tio2-nanoparticles-in-primary-cultured-rat-sertoli-cells-are-mediated-via-a-dysregulated-ca-2-pkc-p38-mapk-nf-%C3%AE%C2%BAb-cascade
#12
Lingqun Ye, Fashui Hong, Xiao Ze, Lingjuan Li, Yaoming Zhou, Yuguan Ze
Although numerous studies have demonstrated that titanium dioxide nanoparticles (TiO2 NPs) can be accumulated in various animal organs and can cause toxicity, there is currently only limited data regarding reproductive toxicity especially on the toxic mechanisms of TiO2 NPs in Sertoli cells. In order to investigate the mechanism of reproductive toxicity, primary cultured rat Sertoli cells were exposed to 5, 15 or 30 μg/ml TiO2 NPs for 24 h, and TiO2 NPs internalization, expression of PKC (p-PKC) and p38 MAPK (p-p38 MAPK) as well as calcium homeostasis were examined...
February 11, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28176461/high-resolution-localization-of-rab5-eea1-and-nectin-3-to-tubulobulbar-complexes-in-the-rat-testis
#13
Arlo Adams, A Wayne Vogl
Tubulobulbar complexes are clathrin/actin-based structures that internalize intercellular junctions in the testis. They resemble coated pits with extremely long necks that are cuffed by dendritic actin networks. As the structures mature, a swollen region or bulb develops near the end of each complex. The bulbs lack actin cuffs and are closely associated with cisternae of endoplasmic reticulum. The bulbs expand and are internalized and enter endocytic compartments of the Sertoli cell. Previous immunofluorescence studies have demonstrated that markers for early endosomes (Rab5 and EEA1) are associated with tubulobulbar complexes and are localized at or near the ends of the structures...
February 8, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28171822/in-vitro-toxicity-evaluation-of-melamine-on-mouse-tm4-sertoli-cells
#14
Lingling Chang, Jingyuan Wang, Ruiping She, Longhuan Ma, Qiaoxing Wu
The male reproductive toxicity of melamine (MA) has been recognized in recent years excepted for its renal toxicity. Our previous in vivo studies revealed that the damages of Sertoli cell barrier played a critical role in MA-induced testicular toxicity in mice. Herein, we performed an in vitro study to comprehensively evaluate the toxicity of MA on Sertoli cell by examining the influences of MA on the viability, morphology, mortality and intercellular junctions of mouse TM4 Sertoli cells (TM4 cells). The results showed that MA suppressed cell viability, induced obvious ultrastructural changes and cell apoptosis in concentration-dependent manner...
January 15, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28160135/1-25-oh-2-vitamin-d3-signalling-on-immature-rat-sertoli-cells-gamma-glutamyl-transpeptidase-and-glucose-metabolism
#15
Renata Gonçalves, Ariane Zamoner, Leila Zanatta, Ana Paula Zanatta, Aline Pertile Remor, Débora da Luz Scheffer, Alexandra Latini, Fátima Regina Mena Barreto Silva
1α,25-Dihydroxyvitamin D3 (1,25-D3) is critical for the maintenance of normal male reproduction since reduced fertility is observed in vitamin D-deficient rats. Gamma-glutamyl transpeptidase (GGT) is a membrane-bound enzyme that is localized on Sertoli cells and catalyses the transfer of the gamma-glutamyl residues to an amino acid or peptide acceptor. Sertoli cells are also responsible for providing nutrients, as lactate, to the development of germ cells. The aim of this study was to investigate the effect and the mechanism of action of 1,25-D3 on GGT on Sertoli cell functions from 30-day-old immature rat testis...
February 3, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/28154936/cultured-bovine-granulosa-cells-rapidly-lose-important-features-of-their-identity-and-functionality-but-partially-recover-under-long-term-culture-conditions
#16
Vengala Rao Yenuganti, Jens Vanselow
Cell culture models are essential for the detailed study of molecular processes. We analyze the dynamics of changes in a culture model of bovine granulosa cells. The cells were cultured for up to 8 days and analyzed for steroid production and gene expression. According to the expression of the marker genes CDH1, CDH2 and VIM, the cells maintained their mesenchymal character throughout the time of culture. In contrast, the levels of functionally important transcripts and of estradiol and progesterone production were rapidly down-regulated but showed a substantial up-regulation from day 4...
February 2, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28152522/generation-and-characteristics-of-human-sertoli-cell-line-immortalized-by-overexpression-of-human-telomerase
#17
Liping Wen, Qingqing Yuan, Min Sun, Minghui Niu, Hong Wang, Hongyong Fu, Fan Zhou, Chencheng Yao, Xiaobo Wang, Zheng Li, Zuping He
Sertoli cells are required for normal spermatogenesis and they can be reprogrammed to other types of functional cells. However, the number of primary Sertoli cells is rare and human Sertoli cell line is unavailable. In this study, we have for the first time reported a stable human Sertoli cell line, namely hS1 cells, by overexpression of human telomerase. The hS1 cells expressed a number of hallmarks for human Sertoli cells, including SOX9, WT1, GDNF, SCF, BMP4, BMP6, GATA4, and VIM, and they were negative for 3β-HSD, SMA, and VASA...
February 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28150810/differential-lactate-and-cholesterol-synthetic-activities-in-xy-and-xx-sertoli-cells
#18
Yurina Shishido, Takashi Baba, Tetsuya Sato, Yuichi Shima, Kanako Miyabayashi, Miki Inoue, Haruhiko Akiyama, Hiroshi Kimura, Yoshiakira Kanai, Yasuhiro Ishihara, Shogo Haraguchi, Akira Miyazaki, Damjana Rozman, Takeshi Yamazaki, Man-Ho Choi, Yasuyuki Ohkawa, Mikita Suyama, Ken-Ichirou Morohashi
SRY, a sex-determining gene, induces testis development in chromosomally female (XX) individuals. However, mouse XX Sertoli cells carrying Sry (XX/Sry Sertoli cells) are incapable of fully supporting germ cell development, even when the karyotype of the germ cells is XY. While it has therefore been assumed that XX/Sry Sertoli cells are not functionally equivalent to XY Sertoli cells, it has remained unclear which specific functions are affected. To elucidate the functional difference, we compared the gene expression of XY and XX/Sry Sertoli cells...
February 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28144033/reduced-numbers-of-sertoli-germ-and-spermatogonial-stem-cells-in-impaired-spermatogenesis
#19
Anna Hentrich, Martin Wolter, Carolin Szardening-Kirchner, Georg H Lüers, Martin Bergmann, Sabine Kliesch, Lutz Konrad
No abstract text is available yet for this article.
February 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28142151/novel-amh-and-amhr2-mutations-in-two-egyptian-families-with-persistent-m%C3%A3-llerian-duct-syndrome
#20
Inas Mazen, Mona El-Gammal, Ken McElreavey, Aya Elaidy, Mohamed S Abdel-Hamid
Anti-müllerian hormone (AMH) is produced by Sertoli cells and signals through 2 transmembrane receptors (AMHR), specific types I and II, leading to regression of müllerian ducts during fetal male sex differentiation. Mutations in AMH and AMHR2 lead to the persistence of müllerian ducts in males which is transmitted in a recessive pattern. Here, we report 2 Egyptian DSD (disorder of sex development) patients reared as males who presented with bilateral cryptorchidism and otherwise normal male external genitalia and who both had a 46,XY karyotype...
February 1, 2017: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
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