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https://www.readbyqxmd.com/read/28101891/different-expression-of-leptin-and-igf1-in-the-adult-and-prepubertal-testis-in-dogs
#1
L Müller, M P Kowalewski, I M Reichler, E Kollár, O Balogh
Leptin (Lep) and insulin-like growth factor 1 (IGF1) are implicated in the regulation of testicular function, but in dogs, our knowledge is limited to the possible role of the IGF1 system in testicular tumours. In this study, we aimed to describe and compare gene expression and protein localization of Lep, IGF1 and their receptors (LepR and IGF1R, respectively) in the testis of healthy adult and prepubertal dogs. Testes were collected from sexually healthy mature (n = 7) and from 8-week-old dogs (n = 7)...
January 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28101370/recurrent-ovarian-sertoli-leydig-cell-tumor-in-a-child-with-peutz-jeghers-syndrome
#2
Edward J Bellfield, Ramin Alemzadeh
We present a female child with Peutz-Jeghers syndrome (PJS) with a recurrent ovarian Sertoli-Leydig cell tumor (SLCT). SLCTs are relatively rare sex cord neoplasms that can occur in PJS. The patient was an African-American female who first presented at the age of 3 years with precocious puberty, and then at the age of 17 years with abdominal pain and irregular menses. In each case, she had resection of the mass, which included oophorectomy. To our knowledge, this is the first reported case in a child with PJS to have a recurrent ovarian SLCT...
August 2016: Oxford Medical Case Reports
https://www.readbyqxmd.com/read/28100869/gonadotoropin-actions-on-spermatogenesis-and-hormonal-therapies-for-spermatogenic-disorders-review
#3
Koji Shiraishi, Hideyasu Matsuyama
Microdissection testicular sperm extraction and intracytoplasmic sperm injection have made it possible for men with non-obstructive azoospermia (NOA) to conceive a child. A majority of men cannot produce sperm because spermatogenesis per se is believed to be "irreversibly" disturbed. For these men, it and has been thought that any hormonal therapy will be ineffective. Further understandings of endocrinological regulation of spermatogenesis are needed and LH or FSH receptor knock out (KO) mice have revealed the roles of gonadotropin separately...
January 19, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28095297/dual-estrogenic-regulation-of-the-nuclear-progestin-receptor-and-spermatogonial-renewal-during-gilthead-seabream-sparus-aurata-spermatogenesis
#4
François Chauvigné, Janmejay Parhi, Judith Ollé, Joan Cerdà
Studies in teleosts suggest that progestins have crucial functions during early spermatogenesis. However, the role of the different progestin receptors in these mechanisms is poorly understood. In this work, we investigated the expression pattern and hormonal regulation of the classical nuclear progestin receptor (Pgr) in the gilthead seabream at three different stages of spermatogenesis: the resting (postspawning) phase, onset of spermatogenesis, and spermiation. Immunolocalization experiments using a seabream specific Pgr antibody revealed that the receptor was expressed in Sertoli and Leydig cells, and also in a subset of spermatogonia type A, throughout spermatogenesis...
January 14, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28091913/regenerative-potential-of-stem-and-progenitor-cells-from-ischemic-testes-of-c57bl-6-mice-in-culture-and-in-the-model-of-spermatogenesis-suppression-caused-by-busulfan
#5
E G Skurikhin, A V Pakhomova, O V Pershina, L A Ermolaeva, N N Ermakova, V A Krupin, E S Pan, A I Kudryashova, O Yu Rybalkina, V V Zhdanov, V E Goldberg, A M Dygai
The regenerative potential of stem and progenitor cells from ischemic testes of C57Bl/6 mice was studied in vitro (cell culture) and in vivo (mouse model of busulfan-induced suppression of spermatogenesis). Spermatogonial stem cells with phenotypes CD117(-)CD90(+) and CD51(-)CD24(+)CD52(+) from ischemic testes demonstrated 33-fold and 7-fold increments of cell mass and generated colonies in vitro. Epithelial (CD45(-)CD31(-)Sca-1(+)CD49f(+)) and endothelial (CD45(-)CD31(+)) precursors exhibited lower self-renewal capacity...
January 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28090714/transplantation-of-cd51-stem-leydig-cells-a-new-strategy-for-the-treatment-of-testosterone-deficiency
#6
Zhi Jun Zang, Jiancheng Wang, Zhihong Chen, Yan Zhang, Yong Gao, Zhijian Su, Ying Tuo, Yan Liao, Min Zhang, Qunfang Yuan, Chunhua Deng, Mei Hua Jiang, Andy Peng Xiang
Stem Leydig cell (SLC) transplantation could provide a new strategy for treating the testosterone deficiency. Our previous study demonstrated that CD51 (also called integrin αv) might be a putative cell surface marker for SLCs, but the physiological function and efficacy of CD51(+) SLCs treatment remain unclear. Here, we explore the potential therapeutic benefits of CD51(+) SLCs transplantation and whether these transplanted cells can be regulated by the hypothalamic-pituitary-gonadal (HPG) axis. CD51(+) cells were isolated from the testes of 12-weeks-old C57BL/6 mice, and we showed that such cells expressed SLC markers and that they were capable of self-renewal, extensive proliferation, and differentiation into multiple mesenchymal cell lineages and LCs in vitro...
January 16, 2017: Stem Cells
https://www.readbyqxmd.com/read/28077499/dynamic-gnrh-and-hcg-testing-establishment-of-new-diagnostic-reference-levels
#7
A Kirstine Bang, Loa Nordkap, Kristian Almstrup, Lærke Priskorn, Jørgen Holm Petersen, Ewa Rajpert-De Meyts, Anna-Maria Andersson, Anders Juul, Niels Joergensen
: 1. OBJECTIVE: Gonadotropin-releasing hormone (GnRH) and Human Chorionic Gonadotropin (hCG) stimulation-tests may be used to evaluate the pituitary and testicular capacity. Our aim was to evaluate changes in follicular-stimulating hormone (FSH), luteinizing hormone (LH) and Testosterone after GnRH and hCG stimulation in healthy men, and assess the impact of six single nucleotide polymorphisms on the responses. 2. DESIGN: GnRH- and hCG-stimulation tests were performed on 77 healthy men, 18-40 years (reference group) at a specialized andrology referral center...
January 11, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/28073207/intraoperative-ultrasound-guided-enucleation-of-testicular-nodule
#8
Riccardo Boschian, Giovanni Liguori, Stefano Bucci, Michele Bertolotto, Carlo Trombetta
OBJECTIVE: We report a case of enucleation of a non-palpable right testicular lesion found incidentally at testicular ultrasonography during investigations in a patient with azoospermia. MATERIALS AND METHODS: In 2011 bilateral hypoechoic nonpalpable testicular lesions (5 mm and 3 mm to the right, 3 mm to the left) were found in a 28 years old patient, during diagnostic investigations for azoospermia. In March 2016, ultrasonography showed that the diameter of the right major nodule had grown to 12 mm, characterized by increased vascularization and increased texture...
December 30, 2016: Archivio Italiano di Urologia, Andrologia
https://www.readbyqxmd.com/read/28070708/delayed-treatment-of-undescended-testes-may-promote-hypogonadism-and-infertility
#9
Julia Rohayem, Alessandra Luberto, Eberhard Nieschlag, Michael Zitzmann, Sabine Kliesch
CONTEXT: Undescended testes at birth may be caused by testosterone deficiency during fetal development. It is unclear whether the process of failed descent contributes to permanent endocrine impairment. OBJECTIVES: To evaluate the impact of age at treatment of undescended testes on endocrine and spermatogenic testicular function in middle-aged men. PATIENTS AND METHODS: Reproductive hormone and semen data of 357 men with previously undescended testes were evaluated with respect to age at correction of testicular position and compared to those of 709 controls with eutopic testes at birth and normozoospermia...
January 9, 2017: Endocrine
https://www.readbyqxmd.com/read/28067604/central-hypogonadism-due-to-a-giant-silent-fsh-secreting-atypical-pituitary-adenoma-effects-of-adenoma-dissection-and-short-term-leydig-cell-stimulation-by-luteinizing-hormone-lh-and-human-chorionic-gonadotropin-hcg
#10
Daniele Santi, Giorgia Spaggiari, Livio Casarini, Flaminia Fanelli, Marco Mezzullo, Uberto Pagotto, Antonio R M Granata, Cesare Carani, Manuela Simoni
We present a case report of an atypical giant pituitary adenoma secreting follicle-stimulating hormone (FSH). A 55-year-old patient presented for erectile dysfunction, loss of libido and fatigue. The biochemical evaluation showed very high FSH serum levels in the presence of central hypogonadism. Neither testicular enlargement nor increased sperm count was observed, thus a secretion of FSH with reduced biological activity was supposed. The histological examination after neuro-surgery showed an atypical pituitary adenoma with FSH-positive cells...
January 9, 2017: Aging Male: the Official Journal of the International Society for the Study of the Aging Male
https://www.readbyqxmd.com/read/28056997/human-lh-and-hcg-stimulate-differently-the-early-signalling-pathways-but-result-in-equal-testosterone-synthesis-in-mouse-leydig-cells-in-vitro
#11
Laura Riccetti, Francesco De Pascali, Lisa Gilioli, Francesco Potì, Lavinia Beatrice Giva, Marco Marino, Simonetta Tagliavini, Tommaso Trenti, Flaminia Fanelli, Marco Mezzullo, Uberto Pagotto, Manuela Simoni, Livio Casarini
BACKGROUND: Human luteinizing hormone (LH) and chorionic gonadotropin (hCG) are glycoprotein hormones regulating development and reproductive functions by acting on the same receptor (LHCGR). We compared the LH and hCG activity in gonadal cells from male mouse in vitro, i.e. primary Leydig cells, which is a common tool used for gonadotropin bioassay. Murine Leydig cells are naturally expressing the murine LH receptor (mLhr), which binds human LH/hCG. METHODS: Cultured Leydig cells were treated by increasing doses of recombinant LH and hCG, and cell signaling, gene expression and steroid synthesis were evaluated...
January 5, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28045098/effects-of-elevated-%C3%AE-estradiol-levels-on-the-functional-morphology-of-the-testis-new-insights
#12
Myles Leavy, Matthias Trottmann, Bernhard Liedl, Sven Reese, Christian Stief, Benjamin Freitag, John Baugh, Giulio Spagnoli, Sabine Kölle
Elevated estradiol levels are correlated with male infertility. Causes of hyperestrogenism include diseases of the adrenal cortex, testis or medications affecting the hypothalamus-pituitary-gonadal axis. The aim of our study was to elucidate the effects of estradiol treatment on testicular cellular morphology and function, with reference to the treatment regimen received. Testes samples (n = 9) were obtained post-orchiectomy from male-to-female transsexuals within the age range of 26-52 years. Each patient had a minimum of 1-6 years estradiol treatment...
January 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28025148/circadian-clock-and-steroidogenic-related-gene-expression-profiles-in-mouse-leydig-cells-following-dexamethasone-stimulation
#13
Huatao Chen, Lei Gao, Yongjie Xiong, Dan Yang, Cuimei Li, Aihua Wang, Yaping Jin
Previous studies have shown that circadian clock genes are expressed in mammalian testes; however, it remains unclear if the expression patterns of these genes are cyclic. Furthermore, it is unknown whether Leydig cells, the primary androgen secreting cells in the testis, play a role in the rhythmicity of circadian clock and steroidogenic-related gene transcription. Here, we examine the circadian clock of mouse Leydig cells, and the link to steroidogenic-related gene transcription. We confirm, via sampling over a full circadian time (CT) period, a lack of circadian rhythmicity in mouse testes in comparison with the robust gene expression cycling of circadian clock genes in mouse livers...
December 23, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28018460/unusual-malignant-neoplasms-of-ovary-in-children-two-cases-report
#14
Ali Ghribi, Aicha Bouden, Manef Gasmi, Mourad Hamzaoui
Sex cord tumors with annular tubules are known to originate from the sex cord of embryonic gonads that synthesize Sertoli cells, Leydig cells, granulosa cells, and theca cells of the ovarian stroma, while ovarian small cell carcinoma of the hypercalcemic type is a type of neuroendocrine tumor. Both these tumors are uncommon, potentially malignant neoplasms in children. We report the case of a sex cord tumor with annular tubules in an 11-year-old girl and a case of small cell carcinoma of the hypercalcemic type in a 10-year-old girl...
November 2016: Korean Journal of Pediatrics
https://www.readbyqxmd.com/read/28017703/prepubertal-subchronic-exposure-to-soy-milk-and-glyphosate-leads-to-endocrine-disruption
#15
Jessica Nardi, Patricia Bonamigo Moras, Carina Koeppe, Eliane Dallegrave, Mirna Bainy Leal, Luciana Grazziotin Rossato-Grando
Lactose intolerance is characterized by low or inexistent levels of lactase, and the main treatment consists of dietary changes, especially replacing dairy milk by soy milk. Soy contains phytoestrogens, substances with known estrogenic activity, besides, glyphosate-based herbicides are extensively used in soy crops, being frequently a residue in soy beans, bringing to a concern regarding the consumption of soy-based products, especially for children in breastfeeding period with lactose intolerance. This study evaluated the pubertal toxicity of a soy milk rich feeding (supplemented or not with glyphosate, doses of 50 and 100 mg/kg) during prepubertal period in male rats...
December 23, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28017657/direct-reprogramming-of-mouse-fibroblasts-toward-leydig-like-cells-by-defined-factors
#16
Yan Yang, Ziyi Li, Xupeng Wu, Haolin Chen, Wenting Xu, Qi Xiang, Qihao Zhang, Jie Chen, Ren-Shan Ge, Zhijian Su, Yadong Huang
Leydig cells (LCs) play crucial roles in producing testosterone, and their dysfunction leads to male hypogonadism. LC transplantation is a promising alternative therapy for male hypogonadism. However, the source of LCs limits this strategy for clinical applications. Here, we report our success in reprogramming mice fibroblasts into LCs by expressing three transcriptional factors, Dmrt1, Gata4, and Nr5a1. The induced Leydig-like cells (iLCs) expressed steroidogenic genes, had a global gene expression profile similar to that of adult LCs, and acquired androgen synthesis capabilities...
January 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28012864/germline-and-somatic-dicer1-mutations-in-familial-and-sporadic-liver-tumors
#17
Stefano Caruso, Julien Calderaro, Eric Letouzé, Jean-Charles Nault, Gabrielle Couchy, Anais Boulais, Alain Luciani, Elie-Serge Zafrani, Paulette Bioulac-Sage, Olivier Seror, Sandrine Imbeaud, Jessica Zucman-Rossi
BACKGROUND & AIMS: Growing evidence suggests that genetic predisposition significantly increases the risk of hepatocellular carcinoma (HCC), independently from the presence of other risk factors. Here, we report a novel germline DICER1 mutation associated with familial recurrent liver tumors. Next, we aimed to investigate the contribution of constitutional and somatic DICER1 mutations on HCC occurrence. METHODS: We investigated two individuals of a family that developed recurrent well-differentiated hepatocellular tumors over the years...
December 21, 2016: Journal of Hepatology
https://www.readbyqxmd.com/read/28011299/apoptosis-inducing-factor-gene-depletion-inhibits-zearalenone-induced-cell-death-in-a-goat-leydig-cell-line
#18
Diqi Yang, Tingting Jiang, Pengfei Lin, Huatao Chen, Lei Wang, Nan Wang, Fan Zhao, Keqiong Tang, Dong Zhou, Aihua Wang, Yaping Jin
Zearalenone (ZEA) is a contaminant of human food and animal feedstuffs that causes health hazards. However, the signal pathways underlying ZEA toxicity remain elusive. The aims of this study were to determine which pathways are involved in ZEA-induced cell death and investigate the effect of apoptosis inducing factor (AIF) on cell death during ZEA treatment in the immortalized goat Leydig cell line hTERT-GLC. This study showed that ZEA-induced cell death in hTERT-GLCs works via endoplasmic reticulum (ER) stress, the caspase-dependent pathway, the caspase-independent pathway and autophagy...
December 20, 2016: Reproductive Toxicology
https://www.readbyqxmd.com/read/28007637/reproductive-toxicity-of-linuron-following-gestational-exposure-in-rats-and-underlying-mechanisms
#19
Hongwei Ding, Wei Zheng, Hua Han, Xiyin Hu, Binli Hu, Feng Wang, Liyu Su, Hong Li, Yan Li
Linuron is a widely used herbicide in agriculture; its endocrine disruptive toxicity has recently received public attention. This study was designed to examine the developmental toxicity of linuron on the reproductive system of male offspring following maternal exposure. Mother rats received oral gavages of linuron, once daily, at the dose of 0, 50, 100, 150 or 200mg/kg, from gestational day (GD)13 to GD18; gonadal organs from GD20 fetuses were examined. Data indicated that exposed male offspring had a significantly shortened anogenital distance...
January 15, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28000947/a-potential-mechanism-associated-with-lead-induced-testicular-toxicity-in-rats
#20
M A El-Magd, K A Kahilo, N E Nasr, T Kamal, M Shukry, A A Saleh
This study was conducted to investigate the mechanism of lead (Pb)-induced testicular toxicity. We examined the impact of Pb toxicity on 17β-oestradiol (E2), oestrogen receptors (ERs) and aromatase P450 which are key factors in spermatogenesis. Treatment of rats with Pb acetate (PbAc, 50 mg/L in drinking water) significantly reduced sperm count, motility, viability and increased sperm abnormalities along with degenerative changes in seminiferous tubules and Leydig cells. Additionally, administration of PbAc resulted in a significant reduction in serum testosterone, serum and testicular E2 as well as increased level of testicular testosterone...
December 21, 2016: Andrologia
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