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https://www.readbyqxmd.com/read/29304256/dynamics-ultrastructure-and-gene-expression-of-human-in-vitro-organized-testis-cells-from-testicular-sperm-extraction-biopsies
#1
Kathrein von Kopylow, Wolfgang Schulze, Andrea Salzbrunn, Matthias Schaks, Elke Schäfer, Beate Roth, Stefan Schlatt, Andrej-Nikolai Spiess
STUDY QUESTION: Is it possible to induce in-vitro reorganization of primary human testis cells from testicular sperm extraction (TESE) biopsies, maintain their long-term cultivation in a 2D system and identify cellular compositions? SUMMARY ANSWER: In-vitro reorganization of primary human testis cells from TESE biopsies and their long-term cultivation on uncoated cell culture dishes is feasible and the cellular compositions can be uncovered through gene expression and microscopic analyses...
January 3, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29223654/formulation-physical-in%C3%A2-vitro-and-ex%C3%A2-vivo-evaluation-of-nanomedicine-based-chemosterilant-for-non-surgical-castration-of-male-animals
#2
Sasithon Pagseesing, Jakarwan Yostawonkul, Suvimol Surassmo, Suwimon Boonrungsiman, Katawut Namdee, Mattaka Khongkow, Chatwalee Boonthum, Tawin Iempridee, Uracha Rungsardthong Ruktanonchai, Nattika Saengkrit, Kaywalee Chatdarong, Suppawiwat Ponglowhapan, Teerapong Yata
The overpopulation of free-roaming companion animals has become the global crisis. The development and application of a suitable, effective, non-surgical approach for animal sterilization would have an enormous advantage over the current surgical method. The main purpose of this study was to develop and evaluate a novel nanomedicine-based chemosterilant for non-surgical castration of male companion animals. In this study, we first sought to investigate the testicular toxicity of different apoptosis-inducing agents...
December 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/29176590/nanocarrier-mediated-delivery-of-%C3%AE-mangostin-for-non-surgical-castration-of-male-animals
#3
Jakarwan Yostawonkul, Suvimol Surassmo, Katawut Namdee, Mattaka Khongkow, Chatwalee Boonthum, Sasithon Pagseesing, Nattika Saengkrit, Uracha Rungsardthong Ruktanonchai, Kaywalee Chatdarong, Suppawiwat Ponglowhapan, Teerapong Yata
The overpopulation of abandoned and stray companion animals has become a global crisis. The main purpose of this study was to develop a novel nanomedicine-based antifertility compound for non-surgical castration of male animals. Mangosteen (Garcinia mangostana L) pericarp extract has been shown to exhibit anti-fertility property. α-mangostin (AM)-loaded nanostructured lipid carrier (AM-NLC) was developed to improve male germ cell apoptosis. This study was conducted to investigate physicochemical properties of AM-NLC and determine the biological effects of AM-NLC on spermatogonia cells and testicular explants obtained from castrated testes...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29144803/endocrine-manifestations-of-primary-hyperoxaluria
#4
Shatha Murad, Yuval Eisenberg
OBJECTIVE: Primary hyperoxaluria type 1 (PH1) is a rare metabolic disorder of oxalate overproduction. It is associated with urolithiasis and nephrocalcinosis which progress to ESRD and systemic oxalosis. As oxalate deposits in tissues, non-parathyroid hormone (nonPTH) mediated hypercalcemia, oxalate osteopathy, primary hypothyroidism and primary hypogonadism develop. In this review, we will present a case of PH1 and provide an overview of this clinical entity and its endocrine manifestations...
November 16, 2017: Endocrine Practice
https://www.readbyqxmd.com/read/29136520/characterization-of-myeloid-cell-populations-in-human-testes-collected-after-sex-reassignment-surgery
#5
Rosalie Ponte, Franck P Dupuy, Fadi Brimo, Vikram Mehraj, Pierre Brassard, Maud Belanger, Ekaterina Yurchenko, Mohammad-Ali Jenabian, Nicole F Bernard, Jean-Pierre Routy
The testis has been described in animal models as a site of immune privilege, which protects spermatids against tissue damage during inflammation. Myeloid cells, including macrophages and dendritic cells (DC), are defined as key players in the testicular immune privilege in animal models. However, their distribution and frequency in human testis remain poorly described. To overcome the challenges related to tissue sampling, we obtained testicular tissue from men under hormonal therapy who elected to have sex reassignment surgery (SRS)...
October 14, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/28988284/morphological-and-morphometrical-changes-on-adult-wistar-rat-testis-caused-by-chronic-sodium-arsenite-exposure
#6
Anderson Tadeu de Araújo Ramos, Maria Aparecida Silva Diamante, Celina de Almeida Lamas, Heidi Dolder, Fabrícia de Souza Predes
Arsenic is a contaminant that occurs naturally in the environment, and it is related to several diseases, such as cancer and severe metabolic diseases. Sodium arsenite effects on testes rats are not fully understood regarding morphology and stereology; thus, it becomes necessary to evaluate possible changes in these parameters under low concentrations and simulating occupational exposure. Therefore, the aim of this study was to analyze the morphometrical and stereological changes on rat testis treated with sodium arsenite...
October 7, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28926535/testicular-macrophage-origin
#7
Zoltan Fehervari
No abstract text is available yet for this article.
September 19, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28878076/zika-virus-infects-human-sertoli-cells-and-modulates-the-integrity-of-the-in-vitro-blood-testis-barrier-model
#8
David N Siemann, Daniel P Strange, Payal N Maharaj, Pei-Yong Shi, Saguna Verma
Confirmed reports of Zika virus (ZIKV) in human seminal fluid for months after the clearance of viremia suggest the ability of ZIKV to establish persistent infection in the seminiferous tubules, an immune-privileged site in the testis protected by the blood-testis barrier, also called the Sertoli cell (SC) barrier (SCB). However, cellular targets of ZIKV in human testis and mechanisms by which the virus enters seminiferous tubules remain unclear. We demonstrate that primary human SCs were highly susceptible to ZIKV compared to the closely related dengue virus and induced the expression of alpha interferon (IFN-α), key cytokines, and cell adhesion molecules (vascular cell adhesion molecule 1 [VCAM-1] and intracellular adhesion molecule 1 [ICAM-1])...
November 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28561756/local-actions-of-melatonin-in-somatic-cells-of-the-testis
#9
REVIEW
Mónica Beatriz Frungieri, Ricardo Saúl Calandra, Soledad Paola Rossi
The pineal hormone melatonin regulates testicular function through the hypothalamic-adenohypophyseal axis. In addition, direct actions of melatonin in somatic cells of the testis have been described. Melatonin acts as a local modulator of the endocrine activity in Leydig cells. In Sertoli cells, melatonin influences cellular growth, proliferation, energy metabolism and the oxidation state, and consequently may regulate spermatogenesis. These data pinpoint melatonin as a key player in the regulation of testicular physiology (i...
May 31, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28507813/deep-exploration-of-the-immune-infiltrate-and-outcome-prediction-in-testicular-cancer-by-quantitative-multiplexed-immunohistochemistry-and-gene-expression-profiling
#10
Peter J Siska, Romany A N Johnpulle, Alice Zhou, Jennifer Bordeaux, Ju Young Kim, Bashar Dabbas, Naveen Dakappagari, Jeffrey C Rathmell, W Kimryn Rathmell, Alicia K Morgans, Justin M Balko, Douglas B Johnson
Platinum-based chemotherapy is usually curative for patients with testicular germ cell tumors (TGCT), but a subset of patients experience disease progression and poor clinical outcomes. Here, we tested whether immune profiling of TGCT could identify novel prognostic markers and therapeutic targets for this patient cohort. We obtained primary and metastatic TGCT samples from one center. We performed immune profiling using multiplexed fluorescence immunohistochemistry (FIHC) for T-cell subsets and immune checkpoints, and targeted gene expression profiling (Nanostring nCounter Immune panel)...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28488286/steroidogenesis-decline-accompanied-with-reduced-antioxidation-and-endoplasmic-reticulum-stress-in-mice-testes-during-ageing
#11
D Huang, W Wei, F Xie, X Zhu, L Zheng, Z Lv
To gain an understanding of the mechanisms by which Leydig cell steroidogenic function degenerates with ageing, we explored steroidogenic gene expression in relation to antioxidation status and endoplasmic reticulum (ER) stress during the ageing of mice. Expression of StAR, P450scc and other steroidogenic enzymes decreased starting at middle age (12-month-old) compared to that of the young control (3-month-old) mice. The immunohistochemical staining intensity of 3β-HSD for Leydig cells was significantly weaker in the aged (24-month-old) group than that in the young control group...
May 9, 2017: Andrologia
https://www.readbyqxmd.com/read/28461571/characterization-of-the-micro-environment-of-the-testis-that-shapes-the-phenotype-and-function-of-testicular-macrophages
#12
Ming Wang, Monika Fijak, Hamid Hossain, Melanie Markmann, Rolf M Nüsing, Günter Lochnit, Michaela F Hartmann, Stefan A Wudy, Lizong Zhang, Huanpeng Gu, Lutz Konrad, Trinad Chakraborty, Andreas Meinhardt, Sudhanshu Bhushan
Macrophages are important in the activation of innate immune responses and in a tissue-specific manner in the maintenance of organ homeostasis. Testicular macrophages (TM), which reside in the testicular interstitial space, comprise the largest leukocyte population in the testes and are assumed to play a relevant function in maintaining testicular immune privilege. Numerous studies have indicated that the interstitial fluid (IF) surrounding the TM has immunosuppressive properties, which may influence the phenotype of TM...
June 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28405613/rip140-in-monocytes-macrophages-regulates-osteoclast-differentiation-and-bone-homeostasis
#13
Bomi Lee, Urszula T Iwaniec, Russell T Turner, Yi-Wei Lin, Bart L Clarke, Anne Gingery, Li-Na Wei
Osteolytic bone diseases, such as osteoporosis, are characterized by diminished bone quality and increased fracture risk. The therapeutic challenge remains to maintain bone homeostasis with a balance between osteoclast-mediated resorption and osteoblast-mediated formation. Osteoclasts are formed by the fusion of monocyte/macrophage-derived precursors. Here we report, to our knowledge for the first time, that receptor-interacting protein 140 (RIP140) expression in osteoclast precursors and its protein regulation are crucial for osteoclast differentiation, activity, and coupled bone formation...
April 6, 2017: JCI Insight
https://www.readbyqxmd.com/read/28339606/testicular-abnormalities-in-mice-with-y-chromosome-deficiencies
#14
Victor A Ruthig, Torbjoern Nielsen, Jonathan M Riel, Yasuhiro Yamauchi, Egle A Ortega, Quinci Salvador, Monika A Ward
We recently investigated mice with Y chromosome gene contribution limited to two, one, or no Y chromosome genes in respect to their ability to produce haploid round spermatids and live offspring following round spermatid injection. Here we explored the normalcy of germ cells and Sertoli cells within seminiferous tubules, and the interstitial tissue of the testis in these mice. We performed quantitative analysis of spermatogenesis and interstitial tissue on Periodic acid-Schiff and hematoxylin-stained mouse testis sections...
March 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28239382/mouse-testicular-cell-type-specific-antiviral-response-against-mumps-virus-replication
#15
Han Wu, Xiang Zhao, Fei Wang, Qian Jiang, Lili Shi, Maolei Gong, Weihua Liu, Bo Gao, Chengyi Song, Qihan Li, Yongmei Chen, Daishu Han
Mumps virus (MuV) infection has high tropism to the testis and usually leads to orchitis, an etiological factor in male infertility. However, MuV replication in testicular cells and the cellular antiviral responses against MuV are not fully understood. The present study showed that MuV infected the majority of testicular cells, including Leydig cells (LC), testicular macrophages, Sertoli cells (SC), and male germ cells (GC). MuV was replicated at relatively high efficiencies in SC compared with LC and testicular macrophages...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28126346/purification-and-identification-of-25-hydroxycholesterol-in-a-reptile-seasonal-variation-and-hormonal-regulation
#16
Varsha Singh, Manisha Priyam, Mamta Tripathy, Umesh Rai
The present in vitro study, for the first time, demonstrates the production of 25-hydroxycholestrol (25-HC) by testicular macrophages of a non-mammalian vertebrate. The ether extracts of testicular macrophage-conditioned medium (TMCM) were fractionated on a C18 reversed phase high-performance liquid chromatography (HPLC) column using methanol as the mobile phase. The mass spectrometry (MS) fragmentation pattern of HPLC-purified 25-HC was found to be identical to that of authentic 25-HC. Further, a significant seasonal variation in 25-HC concentration was observed with maximal level in regressed and minimal during breeding phase...
June 1, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27976743/microcystin-leucine-arginine-causes-cytotoxic-effects-in-sertoli-cells-resulting-in-reproductive-dysfunction-in-male-mice
#17
Yabing Chen, Yuan Zhou, Jing Wang, Lihui Wang, Zou Xiang, Dongmei Li, Xiaodong Han
Microcystin-leucine arginine (MC-LR) is a potent toxin for Sertoli cells. However, the specific molecular mechanisms of MC-induced cytotoxicity still remain unclear. In this study, we performed a comprehensive analyses of changes of miRNAs and mRNAs in Sertoli cells treated with MC-LR. Through computational approaches, we showed the pivotal roles of differentially expressed miRNAs that were associated with cell metabolism, cellular growth and proliferation, cell-to-cell signaling and interaction and cellular movement...
December 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27925200/production-of-macrophage-inhibitory-factor-mif-by-primary-sertoli-cells-its-possible-involvement-in-migration-of-spermatogonial-cells
#18
COMPARATIVE STUDY
Mahmoud Huleihel, Maram Abofoul-Azab, Yael Abarbanel, Iris Einav, Elyahu Levitas, Eitan Lunenfeld
Macrophage migration inhibitory factor (MIF) is a multifunctional molecule. MIF was originally identified as a T-cell-derived factor responsible for the inhibition of macrophage migration. In testicular tissue of adult rats, MIF is constitutively expressed by Leydig cells under physiological conditions. The aim of this study was to examine MIF levels in testicular homogenates from different aged mice, and the capacity of Sertoli cells to produce it. We also examined MIF involvement in spermatogonial cell migration...
October 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27803997/evaluating-the-effect-of-silver-nanoparticles-on-testes-of-adult-albino-rats-histological-immunohistochemical-and-biochemical-study
#19
Samah M Ahmed, Shaimaa A Abdelrahman, Sally M Shalaby
Silver nanoparticles (AgNPs) are widely used in medicine, however, they have toxic impacts on different organs. AgNPs distribution to the testes was reported, so, we aimed to study the effect of intraperitoneal injection of AgNPs, at different concentrations and different time durations, on adult rat testes. Sixty healthy adult male Wistar albino rats were divided into three groups; control group (Group I) and two experimental groups (Groups II & III), each of which were subdivided into two subgroups. Rats in group II were exposed for 7 days to low and high doses of AgNPs, respectively...
February 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27709649/histological-alterations-in-leydig-cells-and-macrophages-in-azoospermic-men
#20
A M Mahran, D A Elgamal, H H Ghafeer, S A Abdel-Maksoud, A A Farrag
The study aimed to compare the histological features of Leydig cells and macrophages in the testicular interstitium of obstructive versus nonobstructive azoospermia. Thirty-nine azoospermic men undergoing testicular sperm extraction during intracytoplasmic sperm injection were allocated into obstructive azoospermia group (GI) and nonobstructive azoospermia group (GII) which was subdivided into Sertoli cell-only syndrome (GIIA), germ cell arrest (GIIB) and hypospermatogenesis (GIIC) subgroups. Serum LH, FSH and testosterone levels were measured...
October 6, 2016: Andrologia
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