Ryan M Walsh, Raffaele Luongo, Elisa Giacomelli, Gabriele Ciceri, Chelsea Rittenhouse, Antonietta Verrillo, Maura Galimberti, Vittoria Dickinson Bocchi, Youjun Wu, Nan Xu, Simone Mosole, James Muller, Elena Vezzoli, Johannes Jungverdorben, Ting Zhou, Roger A Barker, Elena Cattaneo, Lorenz Studer, Arianna Baggiolini
Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and the expansion of the neocortex. The in vitro generation of oRG is essential to investigate the underlying mechanisms of human neocortical development and expansion. By activating the STAT3 signaling pathway using leukemia inhibitory factor (LIF), which is not expressed in guided cortical organoids, we define a cortical organoid differentiation method from human pluripotent stem cells (hPSCs) that recapitulates the expansion of a progenitor pool into the oSVZ...
April 5, 2024: Cell Reports