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Micrornas

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https://www.readbyqxmd.com/read/29777914/the-full-length-microrna-cluster-in-the-intron-of-large-latency-transcript-is-associated-with-the-virulence-of-pseudorabies-virus
#1
Xin Wang, Mei-Mei Zhang, Kai Yan, Qi Tang, Yi-Quan Wu, Wen-Bo He, Huan-Chun Chen, Zheng-Fei Liu
Pseudorabies virus (PRV), the etiological pathogen of Aujeszky's disease, belongs to the Alphaherpesvirus subfamily. Large latency transcript (LLT), the most abundant PRV transcript, harbors a ~ 4.6 kb microRNA (miRNA) cluster-encoding intron. To investigate the function of the LLT miRNA cluster during the life cycle of PRV, we generated a miRNA cluster mutation virus (PRV-∆miR cluster) and revertant virus. Analysis of the growth kinetics of PRV-ΔmiR cluster-infected cells revealed significantly smaller plaques and lower titers than the wild-type and revertant viruses...
May 16, 2018: Virology
https://www.readbyqxmd.com/read/29777774/mir-125b-5p-is-involved-in-oxygen-and-glucose-deprivation-injury-in-pc-12-cells-via-cbs-h-2-s-pathway
#2
Yaqi Shen, Zhuqing Shen, Lingyan Guo, Qiuyan Zhang, Zhijun Wang, Lei Miao, Minjun Wang, Jian Wu, Wei Guo, Yizhun Zhu
AIMS: Ischemic stroke is one of the leading causes of death worldwide. MicroRNAs (miRNAs) have been reported to be implicated in cerebral hypoxia injury and could serve as a therapeutic target. As the third gasotransmitter, hydrogen sulfide (H2 S) plays a critical role in hypoxia-induced injury in the central nervous system. Cystathionine β-synthase (CBS) is the main enzyme catalyzing the production of H2 S in brain. The objective of this study was to investigate the effect of miR-125b-5p on protecting against oxygen and glucose deprivation (OGD) injury in PC-12 cells by regulating CBS and H2 S generation...
May 16, 2018: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29777751/evaluating-the-susceptibility-of-ago2-loaded-micrornas-to-degradation-by-nucleases-in-vitro
#3
Reyad Elbarbary, Lynne E Maquat
MicroRNAs (miRNAs) comprise a class of small non-coding RNAs that regulate the stability and/or translatability of most protein-coding transcripts. Steady-state levels of mature miRNAs can be controlled through mechanisms that influence their biogenesis and/or decay rates. Pathways that mediate mature miRNA decay are less well understood than those that mediate miRNA biogenesis. We recently described Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay (TumiD) as a cellular pathway that promotes the sequence-specific endonucleolytic decay of miRNAs that harbor a CA and/or UA dinucleotide...
May 16, 2018: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29777734/gga-mir-454-suppresses-infectious-bursal-disease-virus-ibdv-replication-via-directly-targeting-ibdv-genomic-segment-b-and-cellular-suppressors-of-cytokine-signaling-6-socs6
#4
Mengjiao Fu, Bin Wang, Xiang Chen, Zhiyuan He, Yongqiang Wang, Xiaoqi Li, Hong Cao, Shijun J Zheng
MicroRNAs (miRNAs), as post-transcriptional regulators, play important roles in the process of viral infection through inhibiting virus replication or modulating host immune response. However, the role of miRNAs in host response against infectious bursal disease virus (IBDV) infection is still unclear. In this study, we found that gga-miR-454 of the host was decreased in response to IBDV infection and that transfection of DF-1 cells with miR-454 inhibited IBDV replication via directly targeting the specific sequence of IBDV genomic segment B, while blockage of endogenous miR-454 by inhibitors enhanced virus replication...
May 16, 2018: Virus Research
https://www.readbyqxmd.com/read/29777724/novel-mirna-biomarkers-for-genotoxicity-screening-in-mouse
#5
Hiroyuki Oka, Koichi Masuno, Takeki Uehara, Toru Okamoto, Yoshiharu Matsuura, Toru Nakano, Shinpei Yamaguchi
The genotoxic potential of drugs is a serious problem, and its evaluation is one of the most critical processes of drug development. Although the comet assay of compound-exposed tissue is a frequently used genotoxicity test, its high false-positive rate is a major complication, and we consistently obtained false-positive results using the comet assay of mouse liver for nine hepatotoxic non-genotoxins (NGTXs). To identify novel genotoxin (GTX)-specific biomarkers, we screened the expression of 750 microRNAs (miRNAs) in the livers of mice treated with GTXs or NGTXs...
May 16, 2018: Toxicology
https://www.readbyqxmd.com/read/29777710/mir-616-3p-promotes-angiogenesis-and-emt-in-gastric-cancer-via-the-pten-akt-mtor-pathway
#6
Zhen-Hua Wu, Chen Lin, Chen-Chen Liu, Wei-Wei Jiang, Ming-Zhu Huang, Xin Liu, Wei-Jian Guo
Dysregulation of microRNAs has been demonstrated to be involved in a variety of biological events related to cancer, including proliferation, metastasis, angiogenesis and immune escape. miR-616-3p is located on the chromosome region 12q13.3, however, its potential role and clinical implications in gastric cancer remain poorly understood. The current study aimed to investigate the potential role of miR-616-3p in gastric cancer. The results showed that miR-616-3p was up-regulated in cancer tissues. Higher expression of miR-616-3p in tumor tissues also predicted poor prognosis...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29777702/microrna-608-inhibits-human-hepatocellular-carcinoma-cell-proliferation-via-targeting-the-bet-family-protein-brd4
#7
Ling He, Dijuan Meng, Shi-Hu Zhang, Yi Zhang, Zhengming Deng, Lian-Bao Kong
Over-expression of the bromodomain and extraterminal (BET) family protein BRD4 is associated with hepatocellular carcinoma (HCC) progression. In the present study, we indentified a novel putative anti-BRD4 microRNA: microRNA-608 ("miR-608"). In HepG2 cells and primary human HCC cells, over-expression of miR-608, using a lentiviral construct, induced BRD4 downregulation and proliferation inhibition. Conversely, transfection of the miR-608 inhibitor increased BRD4 expression to promote HepG2 cell proliferation...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29777630/microrna-493-is-a-prognostic-factor-in-triple-negative-breast-cancer
#8
Ling Yao, Yirong Liu, Zhigang Cao, Junjing Li, Yanni Huang, Xin Hu, Zhiming Shao
Breast cancer is one of the most common malignant diseases in women, in which triple-negative breast cancer (TNBC) exhibits higher aggressiveness and recurrence rates, while there are no effective targets or tailored treatments for patients with this subtype. Thus, finding effective prognostic markers for TNBC might help clinicians in their ability to care for their patients. We used tissue microarrays (TMAs) to detect miR-493 expression in breast cancer samples. A miRCURY LNATM detection probe specific for miR-493 was used in in situ hybridization assays...
May 19, 2018: Cancer Science
https://www.readbyqxmd.com/read/29777484/identification-of-differentially-expressed-micrornas-in-sahiwal-bos-indicus-breed-of-cattle-during-thermal-stress
#9
Gyanendra Singh Sengar, Rajib Deb, Umesh Singh, Vivek Junghare, Saugata Hazra, T V Raja, Rani Alex, Ashish Kumar, R R Alyethodi, Rajiv Kant, Subhash Jakshara, C G Joshi
microRNAs (miRNAs) are a class of small non-coding RNAs that play key roles in post transcriptional gene regulation that influence various fundamental cellular processes, including the cellular responses during environmental stresses. However, perusal of literatures revealed few reports on the differential expression of miRNA during thermal stress in Indian native (Bos indicus) cattle breeds. The present investigation aimed to identify differentially expressed miRNAs during thermal stress in Sahiwal (Bos indicus) dairy cattle breed of India, adapted with tropical climate over a long period of time...
May 18, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29777440/mir-27a-b-regulates-liver-regeneration-by-posttranscriptional-modification-of-tmub1
#10
Xiang Lan, Guangyao Li, Hongming Liu, Hangwei Fu, Ping Chen, Menggang Liu
BACKGROUND: Transmembrane and ubiquitin-like domain-containing 1 protein (Tmub1) negatively regulates liver regeneration. However, whether this regulation involves posttranscriptional modification of Tmub1 expression is unknown. AIM: The aim of the study was to investigate whether microRNA (miR)-27a/b regulates posttranscriptional modification of Tmub1 and cell proliferation during liver regeneration. METHODS: Tmub1 mRNA 3'-untranslated region (UTR) sequences were analyzed using online software...
May 17, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29777401/bantam-regulates-the-axonal-geometry-of-drosophila-larval-brain-by-modulating-actin-regulator-enabled
#11
Animesh Banerjee, Jagat Kumar Roy
During development, axonogenesis, an integral part of neurogenesis, is based on well-concerted events comprising generation, rearrangement, migration, elongation, and adhesion of neurons. Actin, specifically the crosstalk between the guardians of actin polymerization, like enabled, chickadee, capping protein plays an essential role in crafting several events of axonogenesis. Recent evidences reflect multifaceted role of microRNA during axonogenesis. Here, we investigated the role of bantam miRNA, a well-established miRNA in Drosophila, in regulating the actin organization during brain development...
May 18, 2018: Invertebrate Neuroscience: IN
https://www.readbyqxmd.com/read/29777114/microrna-expression-profile-of-human-advanced-coronary-atherosclerotic-plaques
#12
Mariana S Parahuleva, Christoph Lipps, Behnoush Parviz, Hans Hölschermann, Bernhard Schieffer, Rainer Schulz, Gerhild Euler
MicroRNA (miR) is reported to be involved in vascular inflammation and may represent a novel class of diagnostic biomarkers in cardiovascular disease. We aimed to identify the miR expression profile in human advanced coronary atherosclerotic plaques (CAP) and to connect this expression to the processes in atherosclerosis. Microarray techniques and TaqMan polymerase chain reaction were used to analyse the global expression of 352 miRs in CAP obtained during ACS MULTI-LINK study. 11 miRs were selected on the basis of their implication in atherosclerosis, endothelial activation, and inflammation...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29777112/micrornas-as-potential-therapeutics-to-enhance-chemosensitivity-in-advanced-prostate-cancer
#13
Hui-Ming Lin, Iva Nikolic, Jessica Yang, Lesley Castillo, Niantao Deng, Chia-Ling Chan, Nicole K Yeung, Eoin Dodson, Benjamin Elsworth, Calan Spielman, Brian Y Lee, Zoe Boyer, Kaylene J Simpson, Roger J Daly, Lisa G Horvath, Alexander Swarbrick
Docetaxel and cabazitaxel are taxane chemotherapy treatments for metastatic castration-resistant prostate cancer (CRPC). However, therapeutic resistance remains a major issue. MicroRNAs are short non-coding RNAs that can silence multiple genes, regulating several signalling pathways simultaneously. Therefore, synthetic microRNAs may have therapeutic potential in CRPC by regulating genes involved in taxane response and minimise compensatory mechanisms that cause taxane resistance. To identify microRNAs that can improve the efficacy of taxanes in CRPC, we performed a genome-wide screen of 1280 microRNAs in the CRPC cell lines PC3 and DU145 in combination with docetaxel or cabazitaxel treatment...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29777111/knockdown-of-rna-interference-pathway-genes-impacts-the-fitness-of-western-corn-rootworm
#14
Courtney Davis-Vogel, Angel Ortiz, Lisa Procyk, Jonathan Robeson, Adane Kassa, Yiwei Wang, Emily Huang, Carl Walker, Amit Sethi, Mark E Nelson, Dipali G Sashital
Western corn rootworm (Diabrotica virgifera virgifera) is a serious agricultural pest known for its high adaptability to various management strategies, giving rise to a continual need for new control options. Transgenic maize expressing insecticidal RNAs represents a novel mode of action for rootworm management that is dependent on the RNA interference (RNAi) pathways of the insect for efficacy. Preliminary evidence suggests that western corn rootworm could develop broad resistance to all insecticidal RNAs through changes in RNAi pathway genes; however, the likelihood of field-evolved resistance occurring through this mechanism remains unclear...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29777028/microrna-302-cluster-downregulates-enterovirus-71-induced-innate-immune-response-by-targeting-kpna2
#15
Nanfang Peng, Xuecheng Yang, Chengliang Zhu, Li Zhou, Haisheng Yu, Mengqi Li, Yong Lin, Xueyu Wang, Qian Li, Yinglong She, Jun Wang, Qian Zhao, Mengji Lu, Ying Zhu, Shi Liu
Enterovirus 71 (EV71) induces significantly elevated levels of cytokines and chemokines, leading to local or systemic inflammation and severe complications. As shown in our previous study, microRNA (miR) 302c regulates influenza A virus-induced IFN expression by targeting NF-κB-inducing kinase. However, little is known about the role of the miR-302 cluster in EV71-mediated proinflammatory responses. In this study, we found that the miR-302 cluster controls EV71-induced cytokine expression. Further studies demonstrated that karyopherin α2 (KPNA2) is a direct target of the miR-302 cluster...
May 18, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29776629/clinical-implication-of-micrornas-in-molecular-pathology-an-update-for-2018
#16
REVIEW
Seema Sethi, Sajiv Sethi, Martin H Bluth
MicroRNAs (miRNAs) are poised to provide diagnostic, prognostic, and therapeutic targets for several diseases including malignancies for precision medicine applications. The miRNAs have immense potential in the clinical arena because they can be detected in the blood, serum, tissues (fresh and formalin-fixed paraffin-embedded), and fine-needle aspirate specimens. The most attractive feature of miRNA-based therapy is that a single miRNA could be useful for targeting multiple genes that are deregulated in cancers, which can be further investigated through systems biology and network analysis that may provide cancer-specific personalized therapy...
June 2018: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/29776561/circulating-micrornas-as-emerging-cardiac-biomarkers-responsive-to-acute-exercise
#17
David de Gonzalo-Calvo, Alberto Dávalos, Manuel Fernández-Sanjurjo, Laura Amado-Rodríguez, Susana Díaz-Coto, Cristina Tomás-Zapico, Ana Montero, Ángela García-González, Vicenta Llorente-Cortés, Maria Eugenia Heras, Araceli Boraita Pérez, Ángel E Díaz-Martínez, Natalia Úbeda, Eduardo Iglesias-Gutiérrez
BACKGROUND: Circulating microRNAs (c-miRNAs) are mediators of intercellular communication with great potential as cardiac biomarkers. The analysis of c-miRNAs in response to physiological stress, such as exercise, would provide valuable information for clinical practice and a deeper understanding of the molecular response to physical activity. Here, we analysed for the first time the acute exercise response of c-miRNAs reported as biomarkers of cardiac disease in a well-characterized cohort of healthy active adults...
August 1, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29775892/microrna-135a-inhibits-cardiac-fibrosis-induced-by-isoproterenol-via-trpm7-channel
#18
Yan Wu, Yonghui Liu, Yitong Pan, Chunxiao Lu, Haonan Xu, Xiaozhi Wang, Tingting Liu, Kai Feng, Yiqun Tang
BACKGROUND: Cardiac fibrosis is a crucial factor of heart failure. It has been reported that several microRNAs (miRNAs, miRs) were involved in cardiac fibrosis, however, the role and possible regulatory mechanism of microRNA-135a (miR-135a) in cardiac fibrosis have not been investigated. Here, we explored the regulation mechanism of miR-135a on cardiac fibrosis. METHODS AND RESULTS: In vitro, cardiac fibroblasts (CFs) from neonatal rats were treated by isoproterenol (ISO) at the final concentration of 10 μM for 24 h and miR-135a expression was decreased obviously...
May 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29775853/an-ultrasensitive-electrochemiluminescence-biosensor-for-detection-of-microrna-by-in-situ-electrochemically-generated-copper-nanoclusters-as-luminophore-and-tio-2-as-coreaction-accelerator
#19
Hongxia Liao, Ying Zhou, Yaqin Chai, Ruo Yuan
Herein, we constructed an ultrasensitive electrochemiluminescence (ECL) biosensor for detecting microRNA-21 (miR-21) based on in-situ generation of copper nanoclusters (Cu NCs) as luminophore and titanium dioxide (TiO2 ) as coreaction accelerator. First, numerous AT-rich double-stranded DNA (dsDNA) was produced from the conversion of a small amount of target miR-21 via the combination of exonuclease III (Exo III)-assisted amplification and hybridization chain reaction (HCR), which could reduce the aggregation-caused self-etching effect of Cu NCs and improve the emitting of Cu NCs...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29775785/integration-of-mirna-weighted-gene-co-expression-network-and-mirna-mrna-co-expression-analyses-reveals-potential-regulatory-functions-of-mirnas-in-calf-rumen-development
#20
Duy N Do, Pier-Luc Dudemaine, Bridget E Fomenky, Eveline M Ibeagha-Awemu
This study aimed to explore the roles of microRNAs (miRNAs) in calf rumen development during early life. Rumen tissues were collected from 16 calves (8 at pre-weaning and 8 at post-weaning) for miRNA-sequencing, differential expression (DE), miRNA weighted gene co-expression network (WGCNA) and miRNA-mRNA co-expression analyses. 295 miRNAs were identified. Bta-miR-143, miR-26a, miR-145 and miR-27b were the most abundantly expressed. 122 miRNAs were significantly DE between the pre- and post-weaning periods and the most up- and down-regulated miRNAs were bta-miR-29b and bta-miR-493, respectively...
May 15, 2018: Genomics
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