keyword
MENU ▼
Read by QxMD icon Read
search

Animal surgery

keyword
https://www.readbyqxmd.com/read/29146066/immunological-response-induced-by-cryoablation-against-murine-h22-hepatoma-cell-line-in-vivo
#1
Xueling Yang, Xiaoli Li, Zhi Guo, Tongguo Si, Haipeng Yu, Wenge Xing
OBJECTIVES: To describe immunological consequences induced by cryoablation against H22 cells in vivo. METHODS: Adult BALB/c mice underwent subcutaneous implantation of H22 cells. All of them were assigned into three groups randomly: group A (false surgery), group B (cryoablation) and group C (cryoablation plus Freund's adjuvant). Animals were sacrificed 1, 2 and 3 weeks after treatment. Serum IFN-γ and IL-4, Th1/Th2 in spleens and cytotoxicity were detected. RESULTS: Compared with that of group A, (1) INF-γ of group B was higher, but IL-4 was lower; cryoablation plus Freund's adjuvant enhanced these effects...
November 13, 2017: Cryobiology
https://www.readbyqxmd.com/read/29141940/the-effects-of-different-storage-solutions-on-renal-ischemia-tolerance-after-kidney-transplantation-in-mice
#2
Lei Wang, Jin Wei, Shan Jiang, Hui-Hua Li, Liying Fu, Jie Zhang, Ruisheng Liu
Cold storage is the most prevalent method for graft preservation in kidney transplantation (KTX). The protective effects of various preservation solutions have been extensively studied in both clinical trials and experimental animal models. However, a paucity of studies examined the effect of different preservation solutions on graft function in mouse KTX, in addition, the tolerance of the transplanted grafts to further insult has not been evaluated, which were the objectives of the present study. We performed mouse KTX in three groups with the donor kidneys preserved in different solutions for 60 minutes: saline, mouse serum, and University of Wisconsin (UW) solution...
November 15, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29140982/development-standardization-and-testing-of-a-bacterial-wound-infection-model-based-on-ex-vivo-human-skin
#3
Christoph Schaudinn, Christin Dittmann, Jana Jurisch, Michael Laue, Nazende Günday-Türeli, Ulrike Blume-Peytavi, Annika Vogt, Fiorenza Rancan
Current research on wound infections is primarily conducted on animal models, which limits direct transferability of these studies to humans. Some of these limitations can be overcome by using-otherwise discarded-skin from cosmetic surgeries. Superficial wounds are induced in fresh ex vivo skin, followed by intradermal injection of Pseudomonas aeruginosa under the wound. Subsequently, the infected skin is incubated for 20 hours at 37°C and the CFU/wound are determined. Within 20 hours, the bacteria count increased from 107 to 109 bacteria per wound, while microscopy revealed a dense bacterial community in the collagen network of the upper wound layers as well as numerous bacteria scattered in the dermis...
2017: PloS One
https://www.readbyqxmd.com/read/29140580/enhanced-biological-fixation-of-ligaments-to-bone-tissues-utilizing-chitin-fabrics
#4
Hisaya Nomata, Michiko Nakaishi, Kazuo Takakuda
In ligament reconstruction involving anterior cruciate ligament surgery, biological fixation between the transferred ligament and bone tissue is critical for achieving successful outcomes. Here, we administered chitin fabrics into the bone tunnels and evaluated their efficacy in promoting biological fixation. An animal model on the rat's patellar ligament was employed. First, bone tunnels were created in the lateral condyle of the femur. The ligament was then separated from the tibial tuberosity, and half was inserted into the tunnel and fixed with the use of end button...
November 15, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29139571/human-prosthetic-joint-infections-are-associated-with-myeloid-derived-suppressor-cells-mdscs-implications-for-infection-persistence
#5
Cortney E Heim, Debbie Vidlak, Jessica Odvody, Curtis W Hartman, Kevin L Garvin, Tammy Kielian
Prosthetic joint infection (PJI) is a devastating complication of joint arthroplasty surgery typified by biofilm formation. Currently, mechanisms whereby biofilms persist and evade immune-mediated clearance in immune competent patients remain largely ill-defined. Therefore, the current study characterized leukocyte infiltrates and inflammatory mediator expression in tissues from patients with PJI compared to aseptic loosening. CD33(+) HLA-DR(-) CD66b(+) CD14(-/low) granulocytic myeloid-derived suppressor cells (G-MDSCs) were the predominant leukocyte population at sites of human PJI compared to aseptic tissues...
November 15, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29137871/-anesthesia-and-alzheimer-s-disease-current-perceptions
#6
REVIEW
Ana Filipa Vieira da Silva Ferreira Marques, Teresa Alexandra Santos Carvalho Lapa
BACKGROUND AND OBJECTIVES: It has been speculated that the use of anesthetic agents may be a risk factor for the development of Alzheimer's disease. The objective of this review is to describe and discuss pre-clinical and clinical data related to anesthesia and this disease. CONTENT: Alzheimer's disease affects about 5% of the population over 65 years old, with age being the main risk factor and being associated with a high morbidity. Current evidence questions a possible association between anesthesia, surgery, and long-term cognitive effects, including Alzheimer's disease...
November 11, 2017: Revista Brasileira de Anestesiologia
https://www.readbyqxmd.com/read/29137815/local-injection-of-d-lys-3-ghrp-6-in-the-rat-amygdala-dentate-gyrus-or-ventral-tegmental-area-impairs-memory-consolidation
#7
Siamak Beheshti, Neda Aslani
It is well known that the hormone ghrelin affects learning and memory in different experimental models of learning. Though, the effect of antagonism of ghrelin receptor type 1a (GHS-R1a) in various regions of the brain and on different stages of learning has not been examined. In this study the effect of injection of a GHS-R1a selective antagonist (d-Lys-3-GHRP-6) into the basolateral amygdala, dentate gyrus or ventral tegmental area was examined on memory consolidation in the passive avoidance task. Adult male Wistar rats weighing 230-280g were used...
November 4, 2017: Neuropeptides
https://www.readbyqxmd.com/read/29136945/mitochondria-protection-as-a-mechanism-underlying-the-hepatoprotective-effects-of-glycine-in-cholestatic-mice
#8
Reza Heidari, Vahid Ghanbarinejad, Hamidreza Mohammadi, Asrin Ahmadi, Mohammad Mehdi Ommati, Narges Abdoli, Fatemeh Aghaei, Athena Esfandiari, Negar Azarpira, Hossein Niknahad
Cholestasis is the stoppage of bile flow which could lead to serious clinical complications if not managed. Cytotoxic bile acids are involved in the pathogenesis of liver injury during cholestasis. There are no promising pharmacological interventions against cholestasis and its associated complications. This study examined the impact of glycine supplementation on liver mitochondria as a major target of bile acids-induced toxicity during cholestasis. Mice underwent BDL operation and received glycine (0.25% and 1% w:v in drinking water)...
November 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29136734/-functional-recovery-after-recurrent-laryngeal-nerve-injury-on-different-electromyography-thresholds-during-thyroid-surgery
#9
X L Liu, C L Li, Y S Zhao, H Sun
Objective: To discuss the functional recovery after recurrent laryngeal nerve injury (RLNI) on different electromyography thresholds during thyroid surgery. Methods: The prospective experimentally were induced in 12 acute recurrent laryngeal traction animals (porcine) from December 2014 to December 2015, the amplitude and latency of electromyography, even time course during RLNI and recovery of 24 recurrent laryngeal nerves(RLN) were continuous intraoperative neuromonitoring(IONM), including 12 RLN releasing traction after 50% amplitude decrease (AD) and other 12 RLN after 70% AD...
November 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/29136520/characterization-of-myeloid-cell-populations-in-human-testes-collected-after-sex-reassignment-surgery
#10
Rosalie Ponte, Franck P Dupuy, Fadi Brimo, Vikram Mehraj, Pierre Brassard, Maud Belanger, Ekaterina Yurchenko, Mohammad-Ali Jenabian, Nicole F Bernard, Jean-Pierre Routy
The testis has been described in animal models as a site of immune privilege, which protects spermatids against tissue damage during inflammation. Myeloid cells, including macrophages and dendritic cells (DC), are defined as key players in the testicular immune privilege in animal models. However, their distribution and frequency in human testis remain poorly described. To overcome the challenges related to tissue sampling, we obtained testicular tissue from men under hormonal therapy who elected to have sex reassignment surgery (SRS)...
October 14, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/29135038/comparison-of-iatrogenic-articular-cartilage-injury-in-canine-stifle-arthroscopy-versus-medial-parapatellar-mini-arthrotomy-in-a-cadaveric-model
#11
Cleo P Rogatko, Jennifer J Warnock, Gerd Bobe, Valentine D Verpaalen
OBJECTIVE: To assess iatrogenic articular cartilage injury (IACI) resulting from arthroscopy versus medial parapatellar mini-arthrotomy of the stifle. STUDY DESIGN: Paired comparison of canine cadaver stifles treated with arthroscopy or mini-arthrotomy ANIMALS: Paired canine stifles from 14 cadavers (≥20 kg). METHODS: Stifles (N = 28) were assigned to arthroscopy or arthrotomy. Full stifle joint exploration and meniscal probing were performed...
November 14, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/29134609/expression-of-p27-kip1-and-p18-ink4c-in-human-multiple-endocrine-neoplasia-type-1-related-pancreatic-neuroendocrine-tumors
#12
E B Conemans, G M Raicu-Ionita, C R C Pieterman, K M A Dreijerink, O M Dekkers, A R Hermus, W W de Herder, M L Drent, A N A van der Horst-Schrivers, B Havekes, P H Bisschop, G J Offerhaus, I H M Borel Rinkes, G D Valk, H Th M Timmers, M R Vriens
PURPOSE: Pancreatic neuroendocrine tumors are a major manifestation of multiple endocrine neoplasia type 1 (MEN1). This tumor syndrome is caused by germline mutations in MEN1, encoding menin. Insight into pathogenesis of these tumors might lead to new biomarkers and therapeutic targets for these patients. Several lines of evidence point towards a role for p27(Kip1) and p18(Ink4c) in MEN1-related tumor development in animal models for MEN1, but their contribution to human MEN1-related pancreatic neuroendocrine tumor development is not known...
November 13, 2017: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/29131858/the-composition-and-initial-evaluation-of-a-grimace-scale-in-ferrets-after-surgical-implantation-of-a-telemetry-probe
#13
Marsinah L Reijgwart, Nico J Schoemaker, Riccardo Pascuzzo, Matthew C Leach, Melanie Stodel, Loes de Nies, Coenraad F M Hendriksen, Miriam van der Meer, Claudia M Vinke, Yvonne R A van Zeeland
Reliable recognition of pain is difficult in ferrets as many currently available parameters are non-specific, inconsistent and/or impractical. Grimace scales have successfully been applied to assess pain in different animal species and might also be applicable to ferrets. To compose a Ferret Grimace Scale (FGS), we studied the facial musculature of ferrets and compared lateral photographs of 19 ferret faces at six time points before and after intraperitoneal telemetry probe implantation. We identified the Action Units (AUs) orbital tightening, nose bulging, cheek bulging, ear changes and whisker retraction as potential indicators of pain in ferrets...
2017: PloS One
https://www.readbyqxmd.com/read/29131572/in-vivo-optical-detection-and-spectral-triangulation-of-carbon-nanotubes
#14
Ching-Wei Lin, Hailing Yang, Stephen R Sanchez, Weiqun Mao, Lan Pang, Kathleen Mary Beckingham, Robert C Bast, R Bruce Weisman
Biocompatible composites of single-walled carbon nanotubes in Matrigel have been surgically implanted into mouse ovaries and then noninvasively detected and located in the first in vivo demonstration of spectral triangulation. This optical method deduces the three-dimensional position of a short-wave IR emission source from the wavelength-dependent attenuation of fluorescence in tissues. Measurements were performed with a second-generation optical scanner that uses an LED matrix emitting at 736 nm for diffuse specimen excitation...
November 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29130944/cholecystokinin-expression-in-the-development-of-postinfarction-heart-failure
#15
Xiaoying Dong, Can Wang, Jingqi Zhang, Siyue Wang, Hongjian Li, Yang Kang, Shuangquan Tian, Lu Fu
BACKGROUND/AIMS: Cholecystokinin (CCK) is expressed in cardiomyocytes and may also play an important role in cardiovascular regulation. Clinical studies have shown that plasma CCK levels are an independent marker of cardiovascular mortality in cardiac disease. However, whether the development of postinfarction heart failure is associated with changes in CCK expression is unknown. METHODS: To investigate CCK expression patterns and the association between CCK expression and heart functional parameters, we randomized male Sprague-Dawley rats into myocardial infarction (MI) or sham operation (SO) groups...
October 31, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29130506/animal-models-of-acquired-epilepsy-insights-into-mechanisms-of-human-epileptogenesis
#16
Albert J Becker
In many patients who suffer from epilepsies, recurrent epileptic seizures do not start at birth but develop later in life. This holds particularly true for epilepsies with a focal seizure origin including focal cortical dysplasias (FCDs) and temporal lobe epilepsy (TLE). TLE most frequently has its seizure onset in the hippocampal formation. Hippocampal biopsies of pharmacoresistant TLE patients undergoing epilepsy surgery for seizure control most frequently reveal the damage pattern of hippocampal sclerosis, i...
November 12, 2017: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/29128683/diy-do-imaging-yourself-conventional-microscopes-as-powerful-tools-for-in-vivo-investigation
#17
Maísa Mota Antunes, Érika de Carvalho, Gustavo Batista Menezes
Intravital imaging has been increasingly employed in cell biology studies and it is becoming one of the most powerful tools for in vivo investigation. Although some protocols can be extremely complex, most intravital imaging procedures can be performed using basic surgery and animal maintenance techniques. More importantly, regular confocal microscopes - the same that are used for imaging immunofluorescence slides - can also acquire high quality intravital images and movies after minor adaptations. Here we propose minimal adaptations in stock microscopes that allow major improvements in different fields of scientific investigation...
November 8, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29128374/agreement-between-patient-based-and-clinician-based-assessment-of-the-shoulder
#18
REVIEW
Nina K Pattabhiraman, Samuel C Barnes, Harvinder Pal Singh
BACKGROUND: Patient home-based self-assessments after shoulder surgery have the potential to aid clinicians in reducing clinic time and decreasing follow-up requirements. The purpose of this systematic review was to determine the correlation between patient-based and physician-assessed outcome measures for range of motion (ROM), strength, and shoulder function. METHODS: This systematic review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement...
November 8, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/29127739/renal-haemodynamics-and-oxygenation-during-and-after-cardiac-surgery-and-cardiopulmonary-bypass
#19
REVIEW
Roger G Evans, Yugeesh R Lankadeva, Andrew D Cochrane, Bruno Marino, Naoya Iguchi, Michael Z L Zhu, Sally G Hood, Julian A Smith, Rinaldo Bellomo, Bruce S Gardiner, Chang-Joon Lee, David W Smith, Clive N May
Acute kidney injury (AKI) is a common complication following cardiac surgery performed on cardiopulmonary bypass (CPB) and has important implications for prognosis. The aetiology of cardiac surgery-associated AKI is complex, but renal hypoxia, particularly in the medulla, is thought to play at least some role. There is strong evidence from studies in experimental animals, clinical observations and computational models, that medullary ischaemia and hypoxia occurs during CPB. There are no validated methods to monitor or improve renal oxygenation during CPB, and thus possibly decrease the risk of AKI...
November 11, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/29126578/the-earliest-blood-transfusions-in-17th-century-in-italy-1667-1668
#20
REVIEW
Silvia Marinozzi, Valentina Gazzaniga, Silvia Iorio
Historical accounts of the earliest experiments in blood transfusion celebrate work done in France and England in 1667 to 1668. Less attention has been given to pioneering experiments conducted at the same period in Italy. We review records of the first blood transfusion experiments conducted in 17th century Italy. Using original source documents, we provide details of early experiments focusing on the identity of early researchers, their instruments, and techniques. Accounts of the period describe animal-to-animal, animal-to-human, and human-to-human transfusions...
September 23, 2017: Transfusion Medicine Reviews
keyword
keyword
41870
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"