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Quorum sensing inhibition, Pseudomonas aeruginosa

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https://www.readbyqxmd.com/read/29767954/development-of-molecularly-imprinted-polymers-to-block-quorum-sensing-and-inhibit-bacterial-biofilm-formation
#1
Luyao Ma, Shaolong Feng, Cesar de la Fuente-Nunez, Robert E W Hancock, Xiaonan Lu
Bacterial biofilms are responsible for most clinical infections and show increased antimicrobial resistance. In this study, molecularly imprinted polymers (MIPs) were developed to specifically capture prototypical quorum sensing autoinducers [i.e., N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12AHL)], interrupt quorum sensing, and subsequently inhibit biofilm formation of Pseudomonas aeruginosa, an important human nosocomial pathogen. The synthesis of MIPs was optimized by considering the amount and type of the functional monomers itaconic acid (IA) and 2-hydroxyethyl methacrylate (HEMA)...
May 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29765621/biofilm-inhibition-and-pathogenicity-attenuation-in-bacteria-by-proteus-mirabilis
#2
Shichen Yu, Xiaoshan Zhu, Jin Zhou, Zhonghua Cai
Biofilms play an important role in the antibiotic resistance of encased bacteria, and biofilm formation is regulated by quorum sensing (QS). Inhibiting the QS system may, therefore, degrade the integrity of a biofilm and expose the bacterial pathogens within it to the deleterious effects of molecules such as antibiotics. Moreover, the use of QS inhibitors (QSIs) may provide a novel approach for treating bacterial infections of aquacultures. In the present study, the bacterium Proteus mirabilis was identified as a potential producer of QSIs...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29755959/pvdq-quorum-quenching-acylase-attenuates-pseudomonas-aeruginosa-virulence-in-a-mouse-model-of-pulmonary-infection
#3
Putri D Utari, Rita Setroikromo, Barbro N Melgert, Wim J Quax
Pseudomonas aeruginosa is the predominant pathogen in pulmonary infections associated with cystic fibrosis. Quorum sensing (QS) systems regulate the production of virulence factors and play an important role in the establishment of successful P. aeruginosa infections. Inhibition of the QS system (termed quorum quenching) renders the bacteria avirulent thus serving as an alternative approach in the development of novel antibiotics. Quorum quenching in Gram negative bacteria can be achieved by preventing the accumulation of N -acyl homoserine lactone (AHL) signaling molecule via enzymatic degradation...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29751512/screening-of-natural-product-derivatives-identifies-two-structurally-related-flavonoids-as-potent-quorum-sensing-inhibitors-against-gram-negative-bacteria
#4
Suvi Manner, Adyary Fallarero
Owing to the failure of conventional antibiotics in biofilm control, alternative approaches are urgently needed. Inhibition of quorum sensing (QS) represents an attractive target since it is involved in several processes essential for biofilm formation. In this study, a compound library of natural product derivatives ( n = 3040) was screened for anti-quorum sensing activity using Chromobacterium violaceum as reporter bacteria. Screening assays, based on QS-mediated violacein production and viability, were performed in parallel to identify non-bactericidal QS inhibitors (QSIs)...
May 3, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29735954/design-and-synthesis-of-lactams-derived-from-mucochloric-and-mucobromic-acids-as-pseudomonas-aeruginosa-quorum-sensing-inhibitors
#5
Basmah Almohaywi, Aditi Taunk, Daniel S Wenholz, Shashidhar Nizalapur, Nripendra N Biswas, Kitty K K Ho, Scott A Rice, George Iskander, David StC Black, Renate Griffith, Naresh Kumar
Bacterial infections, particularly hospital-acquired infections caused by Pseudomonas aeruginosa , have become a global threat with a high mortality rate. Gram-negative bacteria including P. aeruginosa employ N -acyl homoserine lactones (AHLs) as chemical signals to regulate the expression of pathogenic phenotypes through a mechanism called quorum sensing (QS). Recently, strategies targeting bacterial behaviour or QS have received great attention due to their ability to disarm rather than kill pathogenic bacteria, which lowers the evolutionary burden on bacteria and the risk of resistance development...
May 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29733941/anti-quorum-sensing-activity-toxicity-in-zebrafish-danio-rerio-embryos-and-phytochemical-characterization-of-trapa-natans-leaf-extracts
#6
Ivana Aleksic, Petar Ristivojevic, Aleksandar Pavic, Ivana Radojević, Ljiljana R Čomić, Branka Vasiljevic, Dejan Opsenica, Dušanka Milojković-Opsenica, Lidija Senerovic
ETHNOPHARMACOLOGICAL RELEVANCE: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. AIMOF THE STUDY: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts...
May 4, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29694695/quorum-sensing-inhibitor-potential-of-trans-anethole-aganist-pseudomonas-aeruginosa
#7
Demet Hançer Aydemir, Gülşah Çifçi, Viktorya Aviyente, Gülgün Boşgelmez-Tinaz
AIMS: Quorum sensing (QS) is a cell-cell communication system used by a broad spectrum of pathogenic bacteria to control the expression of their virulence genes. The interruption of QS systems of pathogenic bacteria has been considered as a novel way to fight bacterial diseases. In this study, trans-anethole, the main component of anise (Pimpinella anisum) oil was examined for its QS inhibitor (QSI) potential in an attempt to identify novel QSI compound effective against opportunistic pathogen P...
April 25, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29690523/the-effects-of-chinese-herbal-medicines-on-the-quorum-sensing-regulated-virulence-in-pseudomonas-aeruginosa-pao1
#8
Yee Meng Chong, Kah Yan How, Wai Fong Yin, Kok Gan Chan
The quorum sensing (QS) system has been used by many opportunistic pathogenic bacteria to coordinate their virulence determinants in relation to cell-population density. As antibiotic-resistant bacteria are on the rise, interference with QS has been regarded as a novel way to control bacterial infections. As such, many plant-based natural products have been widely explored for their therapeutic roles. These natural products may contain anti-QS compounds that could block QS signals generation or transmission to combat QS pathogens...
April 21, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29680931/cinnamic-acid-attenuates-quorum-sensing-associated-virulence-factors-and-biofilm-formation-in-pseudomonas-aeruginosa-pao1
#9
Jobina Rajkumari, Subhomoi Borkotoky, Ayaluru Murali, Kitlangki Suchiang, Saswat Kumar Mohanty, Siddhardha Busi
OBJECTIVE: Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against Pseudomonas aeruginosa was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein. RESULTS: At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-dependent virulence factors and biofilm formation in P. aeruginosa without affecting the viability of the bacterium. The phytocompound interfered with the initial attachment of planktonic cells to the substratum thereby causing reduction in biofilm development...
April 21, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29662232/characterization-of-aiik-an-ahl-lactonase-from-kurthia-huakui-lam0618-t-and-its-application-in-quorum-quenching-on-pseudomonas-aeruginosa-pao1
#10
Weiwei Dong, Jie Zhu, Xiang Guo, Delong Kong, Qi Zhang, Yiqing Zhou, Xiaoyang Liu, Shumiao Zhao, Zhiyong Ruan
N-Acyl homoserine lactones (AHLs) act as the key quorum sensing (QS) signal molecules in gram-negative bacteria, which coordinates gene expression and then activates various processes, including biofilm formation and production of virulence factors in some pathogens. Quorum quenching (QQ), which is the inactivation of the signal molecules by means of enzymatic degradation or modification, inhibits the processes of QS rather than killing the pathogens and is a promising antipathogenic strategy to control the bacterial pathogens...
April 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29608955/pseudomonas-aeruginosa-pyocyanin-production-reduced-by-quorum-sensing-inhibiting-nanocarriers
#11
Hoang D Lu, Elizabeth Pearson, Kurt D Ristroph, Gregg A Duncan, Laura M Ensign, Jung Soo Suk, Justin Hanes, Robert K Prud'homme
Pseudomonas aeruginosa is an opportunistic gram-negative pathogen that causes a wide range of infections; it is becoming increasingly difficult to treat due to antibiotic resistance. Quorum-sensing (QS) based therapeutics, which function by disabling pathogen virulence without killing pathogens, are a promising class of drugs that may be used to treat bacterial infections without eliciting resistance development. The use of QS drugs to treat pulmonary P. aeruginosa infections, however, has been greatly limited due to the inability to deliver QS drugs at sufficiently high concentrations past physiological barriers such as pulmonary mucus...
March 30, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29596921/n-3-oxododecanoyl-l-homoserine-lactone-modulates-mitochondrial-function-and-suppresses-proliferation-in-intestinal-goblet-cells
#12
Shiyu Tao, Liqiong Niu, Liuping Cai, Yali Geng, Canfeng Hua, Yingdong Ni, Ruqian Zhao
AIMS: The quorum-sensing molecule N‑(3‑oxododecanoyl)‑l‑homoserine lactone (C12-HSL), produced by the Gram negative human pathogenic bacterium Pseudomonas aeruginosa, modulates mammalian cell behavior. Our previous findings suggested that C12-HSL rapidly decreases viability and induces apoptosis in LS174T goblet cells. MAIN METHODS: In this study, the effects of 100 μM C12-HSL on mitochondrial function and cell proliferation in LS174T cells treated for 4 h were evaluated by real-time PCR, enzyme-linked immunosorbent assay (ELISA) and flow cytometry...
March 26, 2018: Life Sciences
https://www.readbyqxmd.com/read/29571725/attenuation-of-quorum-sensing-regulated-virulence-and-biofilm-development-in-pseudomonas-aeruginosa-pao1-by-diaporthe-phaseolorum-ssp12
#13
Subha Swaraj Pattnaik, SampathKumar Ranganathan, Dinakara Rao Ampasala, Asad Syed, Fuad Ameen, Siddhardha Busi
In recent years, Pseudomonas aeruginosa PAO1 emerged as the significant pathogenic microorganism in majority of the hospital-acquired infections due to its resistance to the conventional antibiotics by virtue of its highly organized quorum sensing and associated biofilm formation. In the present study, quorum sensing attenuation potential of Diaporthe phaseolorum SSP12 extract was investigated against P. aeruginosa PAO1 amply supported by molecular docking studies. D. phaseolorum SSP12 extract significantly inhibited the production of LasI/R mediated LasA protease, LasB elastase and chitinase with 66...
March 20, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29535700/a-metabolic-trade-off-modulates-policing-of-social-cheaters-in-populations-of-pseudomonas-aeruginosa
#14
Huicong Yan, Meizhen Wang, Feng Sun, Ajai A Dandekar, Dongsheng Shen, Na Li
Pseudomonas aeruginosa uses quorum sensing (QS) to regulate the production of public goods such as the secreted protease elastase. P. aeruginosa requires the LasI-LasR QS circuit to induce elastase and enable growth on casein as the sole carbon and energy source. The LasI-LasR system also induces a second QS circuit, the RhlI-RhlR system. During growth on casein, LasR-mutant social cheaters emerge, and this can lead to a population collapse. In a minimal medium containing ammonium sulfate as a nitrogen source, populations do not collapse, and cheaters and cooperators reach a stable equilibrium; however, without ammonium sulfate, cheaters overtake the cooperators and populations collapse...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29530588/can-resveratrol-a-quorum-sensing-inhibitor-function-as-an-aminoglycoside-antibiotic-accelerant-against-pseudomonas-aeruginosa
#15
Jin-Wei Zhou, Tong-Tong Chen, Xiao-Juan Tan, Ji-Yang Sheng, Ai-Qun Jia
Pseudomonas aeruginosa infection is a serious disease in cystic fibrosis (CF) patients and is difficult to cure due to biofilm persistence and emerging multidrug resistance. Considering the essential role of quorum sensing (QS) in P. aeruginosa infections, the enhanced effects between quorum sensing inhibitor (QSI) resveratrol and several antibiotics against P. aeruginosa PAO1 were investigated. Crystal violet staining assay revealed that the biofilms of P. aeruginosa PAO1 grown in the presence of resveratrol were more susceptible to aminoglycoside antibiotics...
March 9, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29507088/the-pseudomonas-aeruginosa-prrf1-and-prrf2-small-regulatory-rnas-promote-2-alkyl-4-quinolone-production-through-redundant-regulation-of-the-antr-mrna
#16
Louise Djapgne, Subrata Panja, Luke K Brewer, Jonathan H Gans, Maureen A Kane, Sarah A Woodson, Amanda G Oglesby-Sherrouse
Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that requires iron for growth and virulence. Under low-iron conditions, P. aeruginosa transcribes two highly identical (95%) small regulatory RNAs (sRNAs), PrrF1 and PrrF2, which are required for virulence in acute murine lung infection models. The PrrF sRNAs promote the production of 2-akyl-4(1 H )-quinolone metabolites (AQs) that mediate a range of biological activities, including quorum sensing and polymicrobial interactions. Here, we show that the PrrF1 and PrrF2 sRNAs promote AQ production by redundantly inhibiting translation of antR , which encodes a transcriptional activator of the anthranilate degradation genes...
May 15, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29502217/equisetin-as-potential-quorum-sensing-inhibitor-of-pseudomonas-aeruginosa
#17
Mengmeng Zhang, Mengjia Wang, Xiaocui Zhu, Wengong Yu, Qianhong Gong
OBJECTIVE: To screen for the quorum-sensing (QS) inhibitors from marine-derived fungi and evaluate their anti-QS properties in Pseudomonas aeruginosa. RESULTS: QS inhibitory activity was found in secondary metabolites of a marine fungus Fusarium sp. Z10 using P. aeruginosa QSIS-lasI biosensor. The major active compound of this fungus was isolated by HPLC and identified as equisetin. Subinhibitory concentration of equisetin could inhibit the formation of biofilm, swarming motility, and the production of virulence factors in P...
May 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29482361/effect-of-reserpine-on-pseudomonas-aeruginosa-quorum-sensing-mediated-virulence-factors-and-biofilm-formation
#18
Debaprasad Parai, Malabika Banerjee, Pia Dey, Arindam Chakraborty, Ekramul Islam, Samir Kumar Mukherjee
This study aimed to evaluate the effect of reserpine, a plant-derived indole-alkaloid, against Pseudomonas aeruginosa PAO1 biofilms. The anti-biofilm activity of reserpine was evaluated by crystal violet staining, MTT assay, confocal laser scanning microscopy and scanning electron microscopy. Reserpine effects were also assessed by qRT-PCR of quorum sensing (QS)-regulated genes and biochemical quantification of the QS-mediated virulence factors pyocyanin, rhamnolipids, proteases and elastases. Reserpine reduced biofilm formation, cell motility, virulence factor production, and QS-controlled gene expression...
February 27, 2018: Biofouling
https://www.readbyqxmd.com/read/29467483/clove-bud-oil-modulates-pathogenicity-phenotypes-of-the-opportunistic-human-pathogen-pseudomonas-aeruginosa
#19
Jayalekshmi Haripriyan, Athira Omanakuttan, Nitasha D Menon, Muralidharan Vanuopadath, Sudarslal Sadasivan Nair, Ross Corriden, Bipin G Nair, Victor Nizet, Geetha B Kumar
Earlier studies from our laboratory have demonstrated that clove bud oil (CBO) attenuates expression of certain virulence factors of Pseudomonas aeruginosa PAO1. Here, we probe more deeply into the effect of CBO on four pseudomonal proteases - elastase A, elastase B, protease IV and alkaline protease - each known to play key roles in disease pathogenesis. CBO inhibited the activity of these proteases present in the bacterial culture supernatant. Zymography studies indicated that these proteases can activate host matrix metalloproteases (MMPs) to establish infection, through conversion of pro-MMP-2 to active MMP-2...
February 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29463534/alginate-oligosaccharide-induced-modification-of-the-lasi-lasr-and-rhli-rhlr-quorum-sensing-systems-in-pseudomonas-aeruginosa
#20
Alison A Jack, Saira Khan, Lydia C Powell, Manon F Pritchard, Konrad Beck, Hina Sadh, Lucy Sutton, Alessandra Cavaliere, Hannah Florance, Philip D Rye, David W Thomas, Katja E Hill
Pseudomonas aeruginosa plays a major role in many chronic infections. Its ability to readily form biofilms contributes to its success as an opportunistic pathogen and its resistance/tolerance to antimicrobial/antibiotic therapy. A low-molecular-weight alginate oligomer (OligoG CF-5/20) derived from marine algae has previously been shown to impair motility in P. aeruginosa biofilms and disrupt pseudomonal biofilm assembly. As these bacterial phenotypes are regulated by quorum sensing (QS), we hypothesized that OligoG CF-5/20 may induce alterations in QS signaling in P...
May 2018: Antimicrobial Agents and Chemotherapy
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