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Quorum sensing inhibition, Pseudomonas aeruginosa

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https://www.readbyqxmd.com/read/28164039/quorum-sensing-n-acyl-homoserine-lactones-sdia-suppresses-escherichia-coli-pseudomonas-aeruginosa-conjugation-through-inhibiting-trai-expression
#1
Yang Lu, Jianming Zeng, Binning Wu, Shunmei E, Lina Wang, Renxin Cai, Ni Zhang, Youqiang Li, Xianzhang Huang, Bin Huang, Cha Chen
Conjugation is a key mechanism for horizontal gene transfer and plays an important role in bacterial evolution, especially with respect to antibiotic resistance. However, little is known about the role of donor and recipient cells in regulation of conjugation. Here, using an Escherichia coli (SM10λπ)-Pseudomonas aeruginosa (PAO1) conjugation model, we demonstrated that deficiency of lasI/rhlI, genes associated with generation of the quorum sensing signals N-acyl homoserine lactones (AHLs) in PAO1, or deletion of the AHLs receptor SdiA in the donor SM10λπ both facilitated conjugation...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28149169/anti-quorum-sensing-and-anti-biofilm-activity-of-amomum-tsaoko-amommum-tsao-ko-crevost-et-lemarie-on-foodborne-pathogens
#2
Md Ramim Tanver Rahman, Zaixiang Lou, Fuhao Yu, Peng Wang, Hongxin Wang
Cell-to-cell communication or quorum sensing (QS) leads to biofilm formation and causing other virulence factors which are extreme problems for food safety, biofilm related infectious diseases etc. This study evaluated the anti-QS activity of the Amomum tsaoko extract (0.5-4 mg/ml) by using Chromobacterium violaceum a biosensor strain and biofilm formation by crystal violate assay. Experimental results demonstrated that the overall yield of Amomum tsao-ko extract was 11.33 ± 0.3% (w/w). MIC for Staphylococcus aureus (Gram positive), Salmonella Typhimurium and Pseudomonas aeruginosa (Gram negative) was 1, 2 and 2 mg/ml, respectively...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28144921/comparative-transcriptome-analysis-of-trueperella-pyogenes-reveals-a-novel-antimicrobial-strategy
#3
Kelei Zhao, Wujiao Li, Ting Huang, Xuhao Song, Xiuyue Zhang, Bisong Yue
Trueperella pyogenes is a prevalent opportunistic bacterium that normally causes diverse suppurative lesions, endometritis and pneumonia in various economically important animals. Although the genomic information of this species has been announced, little is known about its functional profiles. In this study, by performing a comparative transcriptome analysis between the highly and moderately virulent T. pyogenes isolates, we found the expression of a LuxR-type DNA-binding response regulator, PloR, was significantly up-regulated in the highly virulent T...
January 31, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28140677/furvina-inhibits-the-3-oxo-c12-hsl-based-quorum-sensing-system-of-pseudomonas-aeruginosa-and-qs-dependent-phenotypes
#4
Anabela Borges, Pedro Sousa, Alexandra Gaspar, Santiago Vilar, Fernanda Borges, Manuel Simões
Disruption of cell-cell communication or quorum sensing (QS) is considered a stimulating approach for reducing bacterial pathogenicity and resistance. Although several QS inhibitors (QSIs) have been discovered so far their clinical use remains distant. This problem can be circumvented by searching for QSI among drugs already approved for the treatment of different diseases. In this context, antibiotics have earned special attention. Whereas at high concentrations antibiotics exert a killing effect, at lower concentrations they may act as signaling molecules and as such can modulate gene expression...
January 31, 2017: Biofouling
https://www.readbyqxmd.com/read/28121162/quorum-sensing-inhibition-in-pseudomonas-aeruginosa-biofilms-new-insights-through-network-mining
#5
Martín Pérez-Pérez, Paula Jorge, Gael Pérez Rodríguez, Maria Olívia Pereira, Anália Lourenço
Quorum sensing plays a pivotal role in Pseudomonas aeruginosa's virulence. This paper reviews experimental results on antimicrobial strategies based on quorum sensing inhibition and discusses current targets in the regulatory network that determines P. aeruginosa biofilm formation and virulence. A bioinformatics framework combining literature mining with information from biomedical ontologies and curated databases was used to create a knowledge network of potential anti-quorum sensing agents for P. aeruginosa...
January 25, 2017: Biofouling
https://www.readbyqxmd.com/read/28119451/flavonoids-suppress-pseudomonas-aeruginosa-virulence-through-allosteric-inhibition-of-quorum-sensing-receptors
#6
Jon E Paczkowski, Sampriti Mukherjee, Amelia R McCready, Jian-Ping Cong, Christopher J Aquino, Hahn Kim, Brad R Henke, Chari D Smith, Bonnie L Bassler
Quorum sensing is a process of cell-cell communication that bacteria use to regulate collective behaviors. Quorum sensing depends on the production, detection, and group-wide response to extracellular signal molecules called autoinducers. In many bacterial species, quorum sensing controls virulence factor production. Thus, disrupting quorum sensing is considered a promising strategy to combat bacterial pathogenicity. Several members of a family of naturally-produced plant metabolites called flavonoids inhibit Pseudomonas aeruginosa biofilm formation by an unknown mechanism...
January 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28096154/influence-of-rhlr-and-lasr-on-polymyxin-pharmacodynamics-in-pseudomonas-aeruginosa-implications-for-quorum-sensing-inhibition-with-azithromycin
#7
Zackery P Bulman, Neang S Ly, Justin R Lenhard, Patricia N Holden, Jürgen B Bulitta, Brian T Tsuji
Quorum sensing's impact on polymyxin and azithromycin pharmacodynamics was assessed in Pseudomonas aeruginosa PAO1 and an isogenic rhlR/lasR double knockout. For polymyxin B, greater killing against the rhlR/lasR knockout versus PAO1 was observed at 10(8) CFU/mL(polymyxin B EC50:5.61 versus 12.5mg/L, respectively; p<0.005). Polymyxin B combined with azithromycin (256mg/L) was synergistic against each strain, significantly reducing the respective polymyxin B EC50 compared to monotherapy (p<0.005), and is a promising strategy to combat P...
January 17, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28094526/-1-h-nmr-based-global-metabolic-studies-of-pseudomonas-aeruginosa-upon-exposure-of-the-quorum-sensing-inhibitor-resveratrol
#8
Tongtong Chen, Jiyang Sheng, Yonghong Fu, Minghui Li, Junsong Wang, Ai-Qun Jia
Quorum sensing (QS) is a process of bacterial communication that has been a novel target for drug discovery. Pyocyanin quantification assay confirmed that resveratrol was an effective quorum sensing inhibitor (QSI) against Pseudomonas aeruginosa PAO1. In this study, the global metabolite changes of P. aeruginosa PAO1 exposed to QSI resveratrol were investigated by (1)H NMR spectroscopy. A total of 40 metabolites containing amino acids, organic acid, organic amine, and energy storage compounds were identified...
January 26, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28075113/pseudomonas-aeruginosa-lasr%C3%A2-dna-binding-is-directly-inhibited-by-quorum-sensing-antagonists
#9
Emma G Suneby, Leslie R Herndon, Tanya L Schneider
Inhibition of quorum sensing in Pseudomonas aeruginosa is of interest as a possible antivirulence strategy for this pathogenic bacterium. The LasR regulator protein is important in coordinating gene expression in response to quorum sensing signaling molecules. One predominant strategy for LasR inhibition is the development of small-molecule antagonists that mimic the native autoinducer, though the mechanism by which they inactivate LasR is not known. This work reveals that multiple antagonists function by binding to and stabilizing LasR in a conformation that renders it unable to bind DNA...
January 11, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28065820/bioavailability-enhanced-resveramax%C3%A2-modulates-quorum-sensing-and-inhibits-biofilm-formation-in-pseudomonas-aeruginosa-pao1
#10
H S Vasavi, H V Sudeep, H B Lingaraju, K Shyam Prasad
Quorum sensing (QS), a cell-to-cell communication mechanism present in many bacterial species plays a key role in regulating the virulence factor and biofilm formation in many pathogens, which causes severe public health risk. Therefore, interfering with QS mechanism offers an attractive strategy to combat bacterial infections. In the present study, anti-QS activity of a novel resveratrol formulation, Resveramax™, was detected using Chromobacterium violaceum biosensor bioassay and the effect of Resveramax on QS-regulated phenotypes in Pseudomonas aeruginosa PAO1 was assessed by standard protocols...
January 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28062182/exposure-of-airway-epithelial-cells-to-pseudomonas-aeruginosa-biofilm-derived-quorum-sensing-molecules-decrease-the-activity-of-the-anti-oxidant-response-element-bound-by-nrf2
#11
Lucie Roussel, Simon Rousseau
Chronic bacterial infections in cystic fibrosis lung disease are often characterized by Pseudomonas aeruginosa biofilms that are regulated by bacterial intercellular signals termed quorum sensing (QS), such as N-(3-oxododecanoyl)-l-homoserine lactone (3OC12-HSL). This study reports that biofilm-derived exoproducts decrease the transcriptional activity of the anti-oxidant response element in bronchial epithelial cells. In a live co-culture assay of BEAS-2B cells and P. aeruginosa biofilm, the QS molecule 3OC12-HSL was an important but not sole contributor to the inhibition of basal NRF2 luciferase reporter activity...
January 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28056745/exploiting-quorum-sensing-inhibition-for-the-control-of-pseudomonas-aeruginosa-and-acinetobacter-baumannii-biofilms
#12
Israel Castillo-Juarez, Luis Esaú López-Jácome, Gloria Soberón-Chávez, María Tomás, Jintae Lee, Paulina Castañeda-Tamez, Iván Ángelo Hernández-Bárragan, Martha Yumiko Cruz-Muñiz, Toshinari Maeda, Thomas K Wood, Rodolfo García-Contreras
Pseudomonas aeruginosa and Acinetobacter baumannii are two of the main bacteria responsible for nosocomial infections; both organisms are resistant to several classes of antibiotics making their infections very difficult to treat. Moreover, they possess a remarkable ability to form biofilms, which further enhances their antimicrobial resistance. Both organisms coordinate their formation of biofilms and their expression of virulence factors through quorum sensing, a system that regulates gene expression at high cell densities and that plays a key role in the establishment of bacterial infections...
January 5, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28035685/a-medicinal-herb-cassia-alata-attenuates-quorum-sensing-in-chromobacterium-violaceum-and-pseudomonas-aeruginosa
#13
P D Rekha, H S Vasavi, C Vipin, K Saptami, A B Arun
Quorum sensing (QS) has been shown to play a crucial role in the pathogenesis in many bacteria and attenuation of QS is one of the targets of antimicrobial therapy with particular interest in combating drug resistance. This study reports the QS inhibitory activity of metabolites from Cassia alata L. (Ca. alata), an important medicinal herb widely used in the treatment of microbial infections. For investigating the QS inhibition (QSI) potential of Ca. alata L., initially, metabolites of the leaves extracted using ethanol were tested against biosensor strain Chromobacterium violaceum CV026 and C...
December 30, 2016: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28033578/fusaric-acid-and-analogues-as-gram-negative-bacterial-quorum-sensing-inhibitors
#14
Truong Thanh Tung, Tim Holm Jakobsen, Trong Tuan Dao, Anja Thoe Fuglsang, Michael Givskov, Søren Brøgger Christensen, John Nielsen
Taking advantage of microwave-assisted synthesis, efficient and expedite procedures for preparation of a library of fusaric acid and 39 analogues are reported. The fusaric acid analogues were tested in cell-based screening assays for inhibition of the las and rhl quorum sensing system in Pseudomonas aeruginosa and the lux quorum sensing system in Vibrio fischeri. Eight of the 40 compounds in the library including fusaric acid inhibited lux quorum sensing and one compound inhibited activity of the las quorum sensing system...
November 24, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28010047/distal-and-proximal-promoters-co-regulate-pqsr-expression-in-pseudomonas-aeruginosa
#15
John M Farrow, Everett C Pesci
The ubiquitous bacterium Pseudomonas aeruginosa is an opportunistic pathogen that can cause serious infections in immunocompromised individuals. P. aeruginosa virulence is controlled partly by intercellular communication, and the transcription factor PqsR is a necessary component in the P. aeruginosa cell-to-cell signaling network. PqsR acts as the receptor for the Pseudomonas quinolone signal, and it controls the production of 2-alkyl-4-quinolone molecules which are important for pathogenicity. Previous studies showed that the expression of pqsR is positively controlled by the quorum-sensing regulator LasR, but it was unclear how LasR is able to induce pqsR transcription...
December 23, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27999062/5-hydroxyethyl-3-tetradecanoyltetramic-acid-represents-a-novel-treatment-for-intravascular-catheter-infections-due-to-staphylococcus-aureus
#16
Marta Zapotoczna, Ewan J Murray, Siobhan Hogan, James P O'Gara, Siri R Chhabra, Weng C Chan, Eoghan O'Neill, Paul Williams
OBJECTIVES: Biofilm infections of intravascular catheters caused by Staphylococcus aureus may be treated with catheter lock solutions (CLSs). Here we investigated the antibacterial activity, cytotoxicity and CLS potential of 5-hydroxyethyl-3-tetradecanoyltetramic acid (5HE-C14-TMA) compared with the related compounds 3-tetradecanoyltetronic (C14-TOA) and 3-tetradecanoylthiotetronic (C14-TTA), which are variants of quorum sensing signalling molecules produced by Pseudomonas aeruginosa METHODS: Antibacterial activity and mechanism of action of 5HE-C14-TMA, C14-TOA and C14-TTA were determined via MIC, bacterial killing, membrane potential and permeability assays...
December 20, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27977197/disulfide-bond-containing-ajoene-analogues-as-novel-quorum-sensing-inhibitors-of-pseudomonas-aeruginosa
#17
July Fong, Mingjun Yuan, Tim Holm Jakobsen, Kim T Mortensen, May Margarette Salido Delos Santos, Song Lin Chua, Liang Yang, Choon Hong Tan, Thomas E Nielsen, Michael Givskov
Since its discovery 22 years ago, the bacterial cell-to-cell communication system, termed quorum sensing (QS), has shown potential as antipathogenic target. Previous studies reported that ajoene from garlic inhibits QS in opportunistic human pathogen Pseudomonas aeruginosa. In this study, screening of an in-house compound library revealed two sulfur-containing compounds which possess structural resemblance with ajoene and inhibit QS in bioreporter assay. Following a quantitative structure-activity relationship (SAR) study, 25 disulfide bond-containing analogues were synthesized and tested for QS inhibition activities...
December 28, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27902410/inhibition-of-quorum-sensing-controlled-virulence-factors-of-pseudomonas-aeruginosa-by-murraya-koenigii-essential-oil-a-study-in-a-caenorhabditis-elegans-infectious-model
#18
P Sankar Ganesh, Ravishankar Vittal Rai
The global emergence of antibiotic-resistant strains of Pseudomonas aeruginosa poses a major threat in both hospital environments and the community. P. aeruginosa is an opportunistic human pathogen, and it also infects a wide range of model organisms including the nematode Caenorhabditis elegans. Quorum sensing (QS) mediates cell-to-cell communication in bacteria and has an important role in regulating virulence genes, antibiotic resistance and biofilm formation, which are crucial for establishment of the infection...
December 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27896412/the-bacterial-quorum-sensing-molecule-n-3-oxo-dodecanoyl-l-homoserine-lactone-inhibits-mediator-release-and-chemotaxis-of-murine-mast-cells
#19
Ibrahim Khambati, Sangsu Han, Daniëlle Pijnenburg, Hannah Jang, Paul Forsythe
OBJECTIVE: Bacterial colonization relies on communication between bacteria via so-called "quorum-sensing molecules", which include the acyl-homoserine lactone group. Certain acyl-homoserine lactones can modulate mammalian cell function and are thought to contribute to bacterial pathogenicity. Given the role of mast cells in host defense, we investigated the ability of acyl-homoserine lactones to modulate mast cell function. METHODS: We utilized murine primary mast cell cultures to assess the effect of acyl-homoserine lactones on degranulation and cytokine release in response to different stimuli...
November 28, 2016: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/27874198/crataeva-nurvala-nanoparticles-inhibit-virulence-factors-and-biofilm-formation-in-clinical-isolates-of-pseudomonas-aeruginosa
#20
Syed Ghazanfar Ali, Mohammad Azam Ansari, Haris M Khan, Mohammad Jalal, Abbas Ali Mahdi, Swaranjit Singh Cameotra
Green synthesized nanoparticles have gained great attention due to their non-toxic and non-hazardous nature. In the present study, bark extract of the medicinal plant in Ayurveda Crataeva nurvala (Buch-Ham) (CN) was chosen for the biosynthesis of silver nanoparticles (AgNPs). These NPs were characterized by Ultra violet visible spectroscopy, Fourier Transform Infra Red, Atomic Force Microscopy, and Transmission Electron Microscopy (TEM). The average particle size of green synthesized CN-AgNPs was 15.2 ± 1...
November 22, 2016: Journal of Basic Microbiology
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