keyword
MENU ▼
Read by QxMD icon Read
search

Quorum sensing inhibition, Pseudomonas aeruginosa

keyword
https://www.readbyqxmd.com/read/28904418/leaf-extracts-of-selected-gardening-trees-can-attenuate-quorum-sensing-and-pathogenicity-of-pseudomonas-aeruginosa-pao1
#1
Kaimin Niu, Min Kuk, Haein Jung, Kokgan Chan, Sooki Kim
An increasing concern on resistance to multiple-antibiotics has led to the discovery of novel agents and the establishment of new precaution strategy. Numerous plant sources have been widely studied to reduce virulence of pathogenic bacteria by interfering cell-to-cell based communication called quorum sensing (QS). Leaf extracts of 17 gardening trees were collected and investigated for their anti-QS effects using a sensor strain Chromobacterium violaceum CV026. Methanolic extracts of K4 (Acer palmatum), K9 (Acer pseudosieboldianum) and K13 (Cercis chinensis) leaves were selected for further experiments based on their antagonism effect on QS without inhibiting C...
September 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28900249/effect-of-efflux-pump-inhibition-on-pseudomonas-aeruginosa-transcriptome-and-virulence
#2
Giordano Rampioni, Cejoice Ramachandran Pillai, Francesca Longo, Roslen Bondì, Valerio Baldelli, Marco Messina, Francesco Imperi, Paolo Visca, Livia Leoni
Efflux pumps of the resistance-nodulation-cell-division (RND) family increase antibiotic resistance in many bacterial pathogens, representing candidate targets for the development of antibiotic adjuvants. RND pumps have also been proposed to contribute to bacterial infection, implying that efflux pump inhibitors (EPIs) could also act as anti-virulence drugs. Nevertheless, EPIs are usually investigated only for their properties as antibiotic adjuvants, while their potential anti-virulence activity is seldom taken into account...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28860065/chitosan-extracted-from-aspergillus-flavus-shows-synergistic-effect-eases-quorum-sensing-mediated-virulence-factors-and-biofilm-against-nosocomial-pathogen-pseudomonas-aeruginosa
#3
Sahira Nsayef Muslim, Israa M S Al Kadmy, Alaa Naseer Mohammed Ali, Batool Kadham Salman, Mohammad Ahmad, Saba Saadoon Khazaal, Nadheema Hammood Hussein, Sraa Nsayef Muslim
The biological methods for extraction of chitosan were used as alternative procedures for chemical methods In biological methods, the chitosan was extracted from A. flavus by using of Lactobacillus paracasei for demineralization and Bacillus subtilis for deproteinization. The yield of extracted chitosan reached to 53.8%, pH was 7.8 and complete solubility in 1% acitic acid. Purified chitosan had the ability to reduce the biofilm forming capacity of P. aeruginosa clearly visible in light microscopic staining and scanning electron microscopy...
August 28, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28851971/a-broad-range-quorum-sensing-inhibitor-working-through-srna-inhibition
#4
Tim H Jakobsen, Anders N Warming, Rebecca M Vejborg, Joana A Moscoso, Marc Stegger, Frederik Lorenzen, Morten Rybtke, Jens B Andersen, Rico Petersen, Paal Skytt Andersen, Thomas E Nielsen, Tim Tolker-Nielsen, Alain Filloux, Hanne Ingmer, Michael Givskov
For the last decade, chemical control of bacterial virulence has received considerable attention. Ajoene, a sulfur-rich molecule from garlic has been shown to reduce expression of key quorum sensing regulated virulence factors in the opportunistic pathogen Pseudomonas aeruginosa. Here we show that the repressing effect of ajoene on quorum sensing occurs by inhibition of small regulatory RNAs (sRNA) in P. aeruginosa as well as in Staphylococcus aureus, another important human pathogen that employs quorum sensing to control virulence gene expression...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28829573/chemical-interrogation-of-luxr-type-quorum-sensing-receptors-reveals-new-insights-into-receptor-selectivity-and-the-potential-for-interspecies-bacterial-signaling
#5
Joseph P Gerdt, Danielle M Wittenwyler, Joshua B Combs, Michelle E Boursier, Jacob W Brummond, He Xu, Helen E Blackwell
Cell-cell signaling between bacteria, including quorum-sensing (QS) communication systems, may play a role in the establishment and maintenance of polymicrobial communities. To better understand and model these interactions, we must uncover the degree to which neighboring species recognize each another's signals. In the current study, we tested the likelihood of whether the QS systems of two opportunistic pathogens (Acinetobacter baumannii and Pseudomonas aeruginosa) that frequently arise in polymicrobial infections would be affected by the QS signals of neighboring species...
September 15, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28818490/inhibition-of-quorum-sensing-controlled-biofilm-formation-in-pseudomonas-aeruginosa-by-quorum-sensing-inhibitors
#6
Ellappan Kalaiarasan, Kottha Thirumalaswamy, Belgode Narasimha Harish, Vasuki Gnanasambandam, Veeresh Kumar Sali, James John
Antimicrobial therapy against extensively drug-resistant (XDR) P. aeruginosa biofilms is less efficient compared to the treatment of equal bacterial counts of free-floating planktonic cells, which has become a serious threat in hospital environment. P. aeruginosa regulate their cooperative activities and physiological processes through a cell to cell chemical communication process called Quorum sensing (QS). This attracted our interest to synthesize, and to chemically characterize two anti-QS compounds, N-(4-{4-fluoroanilno} butanoyl) -l-homoserine lactone (FABHL) and N-(4-{4-chlororoanilno} butanoyl) -l-homoserine lactone (CABHL) to inhibit biofilm formation via disabling the QS circuits...
August 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28810631/hyperoside-inhibits-biofilm-formation-of-pseudomonas-aeruginosa
#7
Yixuan Sun, Fengjun Sun, Wei Feng, Xuewen Qiu, Yao Liu, Bo Yang, Yongchuan Chen, Peiyuan Xia
Pseudomonas aeruginosa (P. aeruginosa) is a common pathogen in hospital-acquired infection and is readily able to form biofilms. Due to its high antibiotic resistance, traditional antibacterial treatments exert a limited effect on P. aeruginosa biofilm infections. It has been indicated that hyperoside inhibits P. aeruginosa PAO1 (PAO1) biofilm formation without affecting growth. Therefore, the current study examined the biofilm formation and quorum sensing (QS) system of PAO1 in the presence of hyperoside. Confocal laser scanning microscopy analysis demonstrated that hyperoside significantly inhibited biofilm formation...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28804019/pseudomonas-aeruginosa-auto-inducer3-oxo-c12-hsl-exerts-bacteriostatic-effect-and-inhibits-staphylococcus-epidermidis-biofilm
#8
Pradeep Kumar Singh, Vivek Kumar Yadav, Manmohit Kalia, Suhaga Dohare, Deepmala Sharma, Vishnu Agarwal
Pseudomonas aeruginosa has evolved the 3-oxo-C12-HSL and C4-HSL based quorum sensing system which is responsible for the regulation of various virulence factors and helps to dominates over other bacterial species. Staphylococcus epidermidis has frequently been reported with P. aeruginosa while the role of C4-HSL and 3-oxo-C12-HSL on the S. epidermidis had widely been unexplored, and as per our knowledge, this is the first report on the impact of C4-HSL and 3-oxo-C12-HSL overS. epidermidis growth and biofilm...
August 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28798903/anti-quorum-sensing-and-anti-biofilm-activity-of-delftia-tsuruhatensis-extract-by-attenuating-the-quorum-sensing-controlled-virulence-factor-production-in-pseudomonas-aeruginosa
#9
Vijay K Singh, Avinash Mishra, Bhavanath Jha
Multidrug-resistance bacteria commonly use cell-to-cell communication that leads to biofilm formation as one of the mechanisms for developing resistance. Quorum sensing inhibition (QSI) is an effective approach for the prevention of biofilm formation. A Gram-negative bacterium, Delftia tsuruhatensis SJ01, was isolated from the rhizosphere of a species of sedge (Cyperus laevigatus) grown along the coastal-saline area. The isolate SJ01 culture and bacterial crude extract showed QSI activity in the biosensor plate containing the reference strain Chromobacterium violaceum CV026...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28738346/synergistic-activity-of-berberine-with-azithromycin-against-pseudomonas-aeruginosa-isolated-from-patients-with-cystic-fibrosis-of-lung-in-vitro-and-in-vivo
#10
YongTao Li, JianRong Huang, LanJuan Li, LinSheng Liu
BACKGROUND/AIMS: Pseudomonas aeruginosa (PA) is one of the major opportunistic pathogens which can cause chronic lung infection of cystic fibrosis (CF). The formation of PA biofilm promotes CF development and restricts the antimicrobial efficacies of current antibiotics. METHODS: The antimicrobial effects of azithromycin (AZM) and berberine (BER) alone and in combination were evaluated using microdilution method, checkerboard assay, time-kill test, qRT-PCR analysis and absorption method...
July 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28729210/deficiency-of-quorum-sensing-system-inhibits-the-resistance-selection-of-pseudomonas-aeruginosa-to-ciprofloxacin-and-levofloxacin-in-vitro
#11
Yating Gao, Jinju Duan, Xin Geng, Zhiqi Zhang, Ruiqin Zhang, Xiaoxia Li, Shuyun Wang, Jianbang Kang, Donghong Yin, Yan Song
OBJECTIVES: The objective was to investigate how deficient of quorum sensing system (QS) affects minimal inhibitory concentrations (MICs) of Pseudomonas aeruginosa strains to ciprofloxacin (CIP) and levofloxacin (LEV) in vitro. METHODS: Collected the clinical Pseudomonas aeruginosa isolates. Screened the strains deficient of QS genes by the PCR agarose electrophoresis, and then randomly picked out normal strains with the same number of deficient ones. All strains incubated on the different CIP-concentration Mueller-Hinton agars for five days, the different concentrations of CIP respectively refered to 0...
September 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28712963/anti-quorum-sensing-activity-of-pistacia-atlantica-against-pseudomonas-aeruginosa-pao1-and-identification-of-its-bioactive-compounds
#12
H Kordbacheh, F Eftekhar, S N Ebrahimi
Pseudomonas aeruginosa is a multidrug resistant opportunistic pathogen and an important cause of nosocomial infections. Quorum-sensing (QS) is a process in which bacterial cell-cell communication can regulates production of many virulence factors including pigment formation and the ability to form biofilm which is essential for establishment of chronic infections. We examined the inhibitory effect of Pistacia atlantica (Anacardiaceae) methanolic leaf extract and its bioactive components on biofilm formation and pigment production by P...
July 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28694794/antivirulent-properties-of-underexplored-cinnamomum-tamala-essential-oil-and-its-synergistic-effects-with-dnase-against-pseudomonas-aeruginosa-biofilms-an-in-vitro-study
#13
Sanaulla Farisa Banu, Durairajan Rubini, Sairam Rakshitaa, Kamaraj Chandrasekar, Ramar Murugan, Aruni Wilson, Shanmugaraj Gowrishankar, Shunmugiah Karutha Pandian, Paramasivam Nithyanand
Pseudomonas aeruginosa is a nosocomial pathogen colonizing patients with chronic infectious diseases and has gained resistance to all the known broad spectrum antibiotics available today. The present study showcases the antibiofilm potential of an essential oil (EO) from an underexplored Cinnamomum species namely, C. tamala, against P. aeruginosa biofilms. Furthermore, the synergistic effects of the EO along with a commercially available DNase (DNaseI) and a DNase (MBD) isolated from a marine bacterium were explored for its antibiofilm activity...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28667333/post-secretional-activation-of-protease-iv-by-quorum-sensing-in-pseudomonas-aeruginosa
#14
Jungmin Oh, Xi-Hui Li, Soo-Kyong Kim, Joon-Hee Lee
Protease IV (PIV), a key virulence factor of Pseudomonas aeruginosa is a secreted lysyl-endopeptidase whose expression is induced by quorum sensing (QS). We found that PIV expressed in QS mutant has severe reduction of activity in culture supernatant (CS), even though it is overexpressed to high level. PIV purified from the QS mutant (M-PIV) had much lower activity than the PIV purified from wild type (P-PIV). We found that the propeptide cleaved from prepro-PIV was co-purified with M-PIV, but never with P-PIV...
June 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28630204/a-low-molecular-weight-alginate-oligosaccharide-disrupts-pseudomonal-microcolony-formation-and-enhances-antibiotic-effectiveness
#15
Manon F Pritchard, Lydia C Powell, Alison A Jack, Kate Powell, Konrad Beck, Hannah Florance, Julian Forton, Philip D Rye, Arne Dessen, Katja E Hill, David W Thomas
In chronic respiratory disease, the formation of dense, 3-dimensional "microcolonies" by Pseudomonas aeruginosa within the airway plays an important role in contributing to resistance to treatment. An in vitro biofilm model of pseudomonal microcolony formation using artificial-sputum (AS) medium was established to study the effects of low-molecular-weight alginate oligomers (OligoG CF-5/20) on pseudomonal growth, microcolony formation, and the efficacy of colistin. The studies employed clinical cystic fibrosis (CF) isolates (n = 3) and reference nonmucoid and mucoid multidrug-resistant (MDR) CF isolates (n = 7)...
September 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28630071/albumin-inhibits-pseudomonas-aeruginosa-quorum-sensing-and-alters-polymicrobial-interactions
#16
Allie Clinton Smith, Anne Rice, Bryan Sutton, Rebecca Gabrilska, Aimee K Wessel, Marvin Whiteley, Kendra P Rumbaugh
Polymicrobial interactions are complex and can influence the course of an infection, as is the case when two or more species exhibit a synergism that produces a disease state not seen with any of the individual species alone. Cell-to-cell signaling is key to many of these interactions, but little is understood about how the host environment influences polymicrobial interactions or signaling between bacteria. Chronic wounds are typically polymicrobial, with Staphylococcus aureus and Pseudomonas aeruginosa being the two most commonly isolated species...
September 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28613253/terpenoids-from-platostoma-rotundifolium-briq-a-j-paton-alter-the-expression-of-quorum-sensing-related-virulence-factors-and-the-formation-of-biofilm-in-pseudomonas-aeruginosa-pao1
#17
Tsiry Rasamiravaka, Jérémie Ngezahayo, Laurent Pottier, Sofia Oliveira Ribeiro, Florence Souard, Léonard Hari, Caroline Stévigny, Mondher El Jaziri, Pierre Duez
Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence properties against an opportunistic bacterial model, Pseudomonas aeruginosa PAO1. Whereas none of the tested extracts exert bacteriostatic and/or bactericidal proprieties, the ethyl acetate and dichloromethane extracts exhibit anti-virulence properties against Pseudomonas aeruginosa PAO1 characterized by an alteration in quorum sensing gene expression and biofilm formation without affecting bacterial viability...
June 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28599132/comparison-of-seven-structurally-related-coumarins-on-the-inhibition-of-quorum-sensing-of-pseudomonas-aeruginosa-and-chromobacterium-violaceum
#18
COMPARATIVE STUDY
R E D'Almeida, R D I Molina, C M Viola, M C Luciardi, C Nieto Peñalver, A Bardón, M E Arena
Quorum sensing (QS) is a cell-to-cell signaling communication system that controls the virulence behavior of a broad spectrum of bacterial pathogens, participating also in the development of biofilms, responsible of the antibiotic ineffectiveness in many infections. Therefore, QS system is an attractive target for antimicrobial therapy. In this study, we compare the effect of seven structurally related coumarins against bacterial growth, biofilm formation and elastase activity of Pseudomonas aeruginosa. In addition, the anti-pathogenic capacity of the seven coumarins was evaluated on the wild type and the biosensor strain of Chromobacterium violaceum...
August 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28596760/in-depth-profiling-of-mvfr-regulated-small-molecules-in-pseudomonas-aeruginosa-after-quorum-sensing-inhibitor-treatment
#19
Giuseppe Allegretta, Christine K Maurer, Jens Eberhard, Damien Maura, Rolf W Hartmann, Laurence Rahme, Martin Empting
Pseudomonas aeruginosa is a Gram-negative bacterium, which causes opportunistic infections in immuno-compromised individuals. Due to its multiple resistances toward antibiotics, the development of new drugs is required. Interfering with Quorum Sensing (QS), a cell-to-cell communication system, has shown to be highly efficient in reducing P. aeruginosa pathogenicity. One of its QS systems employs Pseudomonas Quinolone Signal (PQS) and 4-hydroxy-2-heptylquinoline (HHQ) as signal molecules. Both activate the transcriptional regulator MvfR (Multiple Virulence Factor Regulator), also called PqsR, driving the production of QS molecules as well as toxins and biofilm formation...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28591665/inhibition-of-pseudomonas-aeruginosa-quorum-sensing-by-subinhibitory-concentrations-of-curcumin-with-gentamicin-and-azithromycin
#20
Shahin Bahari, Habib Zeighami, Hesam Mirshahabi, Shekoufeh Roudashti, Fakhri Haghi
OBJECTIVES: Pseudomonas aeruginosa quorum sensing (QS) circuits regulate virulence factors and co-ordinate bacterial pathogenicity. This study aimed to investigate the inhibitory activity of subinhibitory concentrations of curcumin with azithromycin and gentamicin against P. aeruginosa QS-related genes and virulence factors. METHODS: The minimum inhibitory concentrations (MICs) and synergistic activity of curcumin with azithromycin and gentamicin against P. aeruginosa PAO1 were determined using broth microdilution and checkerboard titration methods, respectively...
September 2017: Journal of Global Antimicrobial Resistance
keyword
keyword
41863
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"