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Wash complex

Prem Krishnan Raghupathi, Jerneja Zupančič, Asker Daniel Brejnrod, Samuel Jacquiod, Kurt Houf, Mette Burmølle, Nina Gunde-Cimerman, Søren J Sørensen
Extreme habitats are not only limited to natural environments, but also apply to man-made systems, for instance household appliances such as dishwashers. Limiting factors, such as high temperatures, high and low pH, high NaCl concentrations, presence of detergents and shear force from water during washing cycles define the microbial survival in this extreme system. Fungal and bacterial diversity in biofilms isolated from rubber seals of 24 different household dishwashers were investigated using next generation sequencing...
January 12, 2018: Applied and Environmental Microbiology
Yanfen Fang, Ao Zhou, Wei Yang, Tirusew Araya, Yingping Huang, Ping Zhao, David Johnson, Jianzhu Wang, Zhiyong Jason Ren
This study investigates the adsorption mechanism differences among four nitrogenous dyes, sulforhodamine G (SRG), uncharged/deprotonated rhodamine B (RhB), orange II (Or II) and methyl blue (MB) by montmorillonite (MMT). MMT adsorption capacity for cationic MB was three times that of uncharged RhB and anionic SRG, while anionic Or II was not absorbed. Colloidal MMT particles have two types of surfaces, basal and edge, that interact with nitrogenous dyes very differently. The surface acidity of MMT was characterized with the pyridine adsorption method using in-situ diffuse reflectance infrared Fourier transform spectroscopy (in-situ DRIFTS)...
January 10, 2018: Scientific Reports
Yuanlong Pan, Adam D Kennedy, Thomas J Jönsson, Nortan W Milgram
This study focused on the hypothesis that cognitive decline in aged dogs could be attenuated by dietary supplementation with a nutrient blend consisting of antioxidants, B vitamins, fish oil and l-arginine, referred to hereafter as the Brain Protection Blend (BPB). Baseline cognitive assessment before the start of treatment was used to establish cognitively equivalent control (10·464+2·33 kg) and treatment (12·118+3·386 kg) groups of aged dogs between 9·1 and 11·5 years of age and with body condition score of 5...
January 10, 2018: British Journal of Nutrition
Bin Hu, Pui-Kin So, Yunyun Yang, Jiewei Deng, Yi-Ching Choi, Tiangang Luan, Zhong-Ping Yao
Replacement of capillary with solid substrates for sample loading and ionization has created many new possibilities for electrospray ionization mass spectrometry (ESI-MS). Surface modification is an attractive strategy to enhance the analytical capability of solid-substrate ESI-MS and allow understanding the relationship between surface activity of solid substrates and analytical properties. In this study, we performed surface modification of wooden tips with hydrophobic (-C18), basic (-NH2) and acidic (-SO3H) functional groups and applied various sampling methods, i...
January 4, 2018: Analytical Chemistry
Narciso M Quijada, Evelyne Mann, Martin Wagner, David Rodríguez-Lázaro, Marta Hernández, Stephan Schmitz-Esser
Cheese ripening involves the succession of complex microbial communities that are responsible for the organoleptic properties of the final products. The food processing environment can act as a source of natural microbial inoculation, especially in traditionally manufactured products. Austrian Vorarlberger Bergkäse (VB) is an artisanal washed-rind hard cheese produced in the western part of Austria without the addition of external ripening cultures. Here, the composition of the bacterial communities present on VB rinds and on different processing surfaces from two ripening cellars was assessed by near full length 16S rRNA gene amplification, cloning and sequencing...
December 28, 2017: International Journal of Food Microbiology
Fenlu Zhu, Nirav Shah, Huiqing Xu, Dina Schneider, Rimas Orentas, Boro Dropulic, Parameswaran Hari, Carolyn A Keever-Taylor
BACKGROUND AIMS: Multiple steps are required to produce chimeric antigen receptor (CAR)-T cells, involving subset enrichment or depletion, activation, gene transduction and expansion. Open processing steps that increase risk of contamination and production failure are required. This complex process requires skilled personnel and costly clean-room facilities and infrastructure. Simplified, reproducible CAR-T-cell manufacturing with reduced labor intensity within a closed-system is highly desirable for increased availability for patients...
December 26, 2017: Cytotherapy
Alexander Polster, Stefano Perni, Dilyana Filipova, Ong Moua, Joshua D Ohrtman, Hicham Bichraoui, Kurt G Beam, Symeon Papadopoulos
The type 1 ryanodine receptor (RyR1) in skeletal muscle is a homotetrameric protein that releases Ca2+ from the sarcoplasmic reticulum (SR) in response to an "orthograde" signal from the dihydropyridine receptor (DHPR) in the plasma membrane (PM). Additionally, a "retrograde" signal from RyR1 increases the amplitude of the Ca2+ current produced by CaV1.1, the principle subunit of the DHPR. This bidirectional signaling is thought to depend on physical links, of unknown identity, between the DHPR and RyR1. Here, we investigate whether the isolated cytoplasmic domain of RyR1 can interact structurally or functionally with CaV1...
December 28, 2017: Journal of General Physiology
Daniel P Schofield, William C McGrew, Akiko Takahashi, Satoshi Hirata
Cumulative culture, generally known as the increasing complexity or efficiency of cultural behaviors additively transmitted over successive generations, has been emphasized as a hallmark of human evolution. Recently, reviews of candidates for cumulative culture in nonhuman species have claimed that only humans have cumulative culture. Here, we aim to scrutinize this claim, using current criteria for cumulative culture to re-evaluate overlooked qualitative but longitudinal data from a nonhuman primate, the Japanese monkey (Macaca fuscata)...
December 27, 2017: Primates; Journal of Primatology
Xi Luo, Hongtao Zhao, Yunjie Liao, Xuyong Li
Quantifying road-deposited sediment (RDS) wash-off is critical in estimating pollutant load and for supporting the designs of urban storm water low-impact development (LID) best management practices (BMPs). The objective of this study was to identify a simple and effective small-scale wash-off load estimation and prediction method suitable for BMP engineering. The reliabilities, complexities, and applicabilities of four prediction methods (i.e., the event mean concentration (EMC) equation method, the exponential equation method, and the RDS mass difference before and after rainfall event method, and the RDS index model) were tested for three sites (i...
December 22, 2017: Environmental Science and Pollution Research International
Tomoyuki Moriya, Kazuhiko Fukatsu, Midori Noguchi, Makoto Nishikawa, Hiromi Miyazaki, Daizoh Saitoh, Hideki Ueno, Junji Yamamoto
BACKGROUND: Enteral nutrition (EN) is the gold standard of nutritional therapy for critically ill or severely injured patients, because EN promotes gut and hepatic immunity, thereby preventing infectious complications as compared with parenteral nutrition. However, there are many EN formulas with different protein and fat contents. Their effects on gut-associated lymphoid tissue remain unclear. Recently, semielemental diets (SEDs) containing whey peptides as a nitrogen source have been found to be beneficial in patients with malabsorption or pancreatitis...
February 2018: Journal of Surgical Research
Beth A Bouchard, Thomas Orfeo, Hollis N Keith, Elizabeth M Lavoie, Matthew Gissel, Mark Fung, Kenneth G Mann
BACKGROUND: Intact red blood cells (RBCs) appear to support thrombin generation in in vitro models of blood coagulation. During storage of RBC units, biochemical, structural and physiological changes occur including alterations to RBC membranes and release of microparticles, which are collectively known as storage lesion. The clinical consequences of microparticle formation in RBC units are unclear. This study was performed to assess thrombin generation via the prothrombinase complex by washed RBCs and RBC-derived microparticles as a function of RBC unit age...
December 14, 2017: Journal of Trauma and Acute Care Surgery
Alicia Aleman, Giselle Tomasso, María Luisa Cafferata, Mercedes Colomar, Ana Pilar Betran
INTRODUCTION: It is critical to increase the uptake of interventions proven to be effective to improve maternal and perinatal outcomes. Supply kits have been suggested to be a feasible strategy designed to ensure timely availability and effective follow-up of care. OBJECTIVE: We conducted a systematic review to summarize the evidence on the uptake, effectiveness and safety of supply kits for maternal care. SEARCH STRATEGY: MEDLINE, the Cochrane Pregnancy and Childbirth Group's Trials Register, Campbell Collaboration, Lilacs, Embase and unpublished studies were searched...
December 13, 2017: Reproductive Health
Wenmin Cai, Jiajun Chen, Qingwei Wang, Meng Wei
Presently, the large amount of industrial leaded wastewater creates a great challenge to both environmental governance and wastewater recycling. Lead complexes in washed water must be removed mostly before the washed water can be recycled. This paper reports the mechanism and factors of removing Pb complexes in simulated washed water by the sulfide precipitation method. The reaction time, sodium sulfide dosage, pH, and polymeric aluminum chloride (PAC) dosage were analyzed and the optimal conditions were explored...
December 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Rami Sommerstein, Urs Führer, Elia Lo Priore, Carlo Casanova, Dominik M Meinel, Helena Mb Seth-Smith, Andreas Kronenberg, On Behalf Of Anresis, Daniel Koch, Laurence Senn, Andreas F Widmer, Adrian Egli, Jonas Marschall, On Behalf Of Swissnoso
We describe an outbreak of Burkholderia stabilis associated with contaminated washing gloves, a commercially available Class I medical device. Triggered by an increase in Burkholderia cepacia complex (BCC) bacteremias and the detection of BCC in unopened packages of washing gloves, an ad hoc national outbreak committee comprising representatives of a public health organisation, a regulatory agency, and an expert association convened and commissioned an outbreak investigation. The investigation included retrospective case finding across Switzerland and whole genome sequencing (WGS) of isolates from cases and gloves...
December 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
Joyce Koueik, Brandon G Rocque, Jordan Henry, Taryn Bragg, Jennifer Paul, Bermans J Iskandar
Continuous irrigation is an important adjunct for successful intraventricular endoscopy, particularly for complex cases. It allows better visualization by washing out blood and debris, improves navigation by expanding the ventricles, and assists with tissue dissection. A method of irrigation delivery using a centrifugal pump designed originally for cardiac surgery is presented. The BioMedicus centrifugal pump has the desirable ability to deliver a continuous laminar flow of fluid that excludes air from the system...
December 8, 2017: Journal of Neurosurgery. Pediatrics
Abhishek Ghosh
Complex facial injuries with soft tissue degloving and bony avulsion are very devastating to the patient. Partial degloving injuries are described but hemifacial degloving with zygoma avulsion are rare. The author presents a case of post-traumatic degloving of the left upper lip, nose, part of forehead, upper and lower eyelids and cheek with avulsion of the left zygoma. The management included immediate resuscitation and early surgery to reposition the skeletal as well as soft tissue avulsion. The wound was thoroughly washed and primary repositioning and fixation were done...
September 2017: World Journal of Plastic Surgery
Malorie Mella, Brenda Schweitzer, Sabra Botch-Jones, Claude Mallet
RATIONALE: Analyzing tissue samples is routinely performed when liquid biological samples are not available for replicate analysis. Preparing complex matrices, such as tissue, for analysis can be time-consuming. Traditional sample preparation methods typically begin with homogenization followed by a sample clean up step such as liquid-liquid or solid phase extraction. Samples are typically eluted, evaporated and reconstituted prior to instrumental analysis. The aim of this project was to evaluate multi-dimensional chromatography's utility in reducing the amount of time from sample acquisition to analysis...
December 7, 2017: Rapid Communications in Mass Spectrometry: RCM
Ling-Yun He, Jia-Jia Ou
Pollution emissions impose serious social negative externalities, especially in terms of public health. To reduce pollution emissions cost-effectively, the marginal abatement costs (MACs) of pollution emissions must be determined. Since the industrial sectors are the essential pillars of China's economic growth, as well as leading energy consumers and sulfur dioxide (SO₂) emitters, estimating MACs of SO₂ emissions at the industrial level can provide valuable information for all abatement efforts. This paper tries to address the critical and essential issue in pollution abatement: How do we determine the MACs of pollution emissions in China? This paper first quantifies the SO₂ emission contribution of different industrial sectors in the Chinese economy by an Input-Output method and then estimates MACs of SO₂ for industrial sectors at the national level, provincial level, and sectoral level by the shadow price theory...
December 5, 2017: International Journal of Environmental Research and Public Health
Mona Zamanian Azodi, Mostafa Rezaei Tavirani, Afsaneh Arefi Oskouie, Mostafa Hamdieh, Mohammad Kamran Derakhshan, Alireza Ahmadzadeh, Farid Zayeri, Naser Nejadi, Majid Rezaei Tavirani, Vahid Mansouri, Mohammad Rostami-Nejad, Reza Vafaee
Obsessive-Compulsive Disorder (OCD) is one of the most common mental conditions. Proteome profiling may help identifying important proteins and finally shed lights to complexity of OCD underlying mechanisms. Here, by the application gel-based proteomic approach the proteome profile of patients with washing subtype of OCD before and after treatment with Fluoxetine (positive responders) are compared to healthy matched controls. However, only one of the differentially expressed proteins is examined and introduced in this paper...
2017: Iranian Journal of Pharmaceutical Research: IJPR
Balkis Eddhif, Justin Lange, Nadia Guignard, Yann Batonneau, Jonathan Clarhaut, Sébastien Papot, Claude Geffroy-Rodier, Pauline Poinot
Sample preparation for mass spectrometry-based proteomics is a key step for ensuring reliable data. In gel-free experimental workflows, protein purification often starts with a precipitation stage using trichloroacetic acid (TCA). In presence of TCA, proteins precipitate in a stable molten globule state making the pellet difficult to solubilize in aqueous buffer for proteolytic digestion and MS analysis. In this context, the objective of this work was to study the suitability of a novel agent, ethanol/HCl, for the washing of TCA-precipitated proteins...
November 27, 2017: Journal of Proteomics
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