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https://www.readbyqxmd.com/read/27909246/retromer-wash-dependent-sorting-of-nutrient-transporters-requires-a-multivalent-interaction-network-with-ankrd50
#1
Arunas Kvainickas, Ana Jimenez Orgaz, Heike Nägele, Britta Diedrich, Kate J Heesom, Jörn Dengjel, Peter J Cullen, Florian Steinberg
Retromer and the associated actin polymerizing WASH-complex are essential for the endocytic recycling of a wide range of integral membrane proteins. A hereditary Parkinson's disease causing point mutation (D620N) in the retromer subunit VPS35 perturbs retromer's association with the WASH-complex and also with the uncharacterized protein Ankyrin Repeat Domain Containing Protein 50 (ANKRD50). Here, we firmly establish ANKRD50 as a novel and essential component of the SNX27-retromer-WASH supercomplex. Depletion of ANKRD50 in HeLa or U2OS cells phenocopied the loss of endosome to cell surface recycling of multiple transmembrane proteins seen upon suppression of SNX27, retromer or WASH-complex components...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27904308/laparoscopic-transcystic-treatment-biliary-calculi-by-laser-lithotripsy
#2
Jun Liu, Lan Jin, Zhongtao Zhang
BACKGROUND AND OBJECTIVES: Laparoscopic transcystic common bile duct exploration (LTCBDE) is a complex procedure requiring expertise in laparoscopic and choledochoscopic skills. The purpose of this study was to investigate the safety and feasibility of treating biliary calculi through laparoscopic transcystic exploration of the CBD via an ultrathin choledochoscope combined with dual-frequency laser lithotripsy. METHODS: From August 2011 through September 2014, 89 patients at our hospital were treated for cholecystolithiasis with biliary calculi...
October 2016: JSLS: Journal of the Society of Laparoendoscopic Surgeons
https://www.readbyqxmd.com/read/27890028/the-newly-revised-interview-for-deteriorations-in-daily-living-activities-in-dementia-r-iddd2-distinguishing-initiative-from-performance-at-assessment
#3
Clarissa M Giebel, David Challis, Daniela Montaldi
BACKGROUND: Minimal evidence exists on the detailed deficits in complex instrumental activities of daily living (IADLs) in mild dementia. The aim of this study was twofold, to validate a revised questionnaire focusing measuring the initiative and performance of IADLs in mild dementia and to explore the relationship between individual IADLs and patient and carer well-being. METHODS: A total of 183 carers of people with mild dementia completed a further modified Revised Interview for Deterioration in Daily Living Activities 2 (R-IDDD2), which comprised new activities such as computer use, as well as sub-activities on the performance scale...
November 28, 2016: International Psychogeriatrics
https://www.readbyqxmd.com/read/27884932/drosophila-wash-is-required-for-integrin-mediated-cell-adhesion-cell-motility-and-lysosomal-neutralization
#4
Benedikt M Nagel, Meike Bechtold, Luis Garcia Rodriguez, Sven Bogdan
The Wiskott-Aldrich Syndrome Protein and SCAR Homologue (WASH) is a conserved actin nucleation promoting factor controlling Arp2/3 complex activity in endosomal sorting and recycling. Previous studies have identified WASH as an essential regulator in Drosophila development. Here, we show that homozygous wash mutant flies are viable and fertile. We demonstrate that Drosophila WASH has conserved functions in integrin receptor recycling and lysosome neutralization. WASH generates actin patches on endosomes and lysosomes mediating both functions...
November 24, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27877879/a-stem-less-probe-using-spontaneous-pairing-between-cy3-and-quencher-for-rna-detection
#5
Hiromu Kashida, Kazuhiro Morimoto, Hiroyuki Asanuma
We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target, these dyes formed a complex, and emission of Cy3 was efficiently quenched. Hybridization with the target RNA disrupted this interaction and resulted in Cy3 emission. Under optimized conditions, the signal to background ratio was as high as 180. We demonstrated specific detection of target RNA in cells using a wash-free FISH protocol...
2016: Science and Technology of Advanced Materials
https://www.readbyqxmd.com/read/27857373/chiral-particles-in-the-dual-beam-optical-trap
#6
Oto Brzobohatý, Raúl Josué Hernández, Stephen Simpson, Alfredo Mazzulla, Gabriella Cipparrone, Pavel Zemánek
We investigate the dynamics of chiral microparticles in a dual-beam optical trap. The chiral particles have the structure of spherical chiral microresonators, with a reflectance deriving from the supramolecular helicoidal arrangement. Due to the strong asymmetric response of the particles to light with a specific helicity and wavelength, their trapping position and rotational frequency can be controlled by proper combination of the polarization state of the two light beams. Here symmetric and asymmetric polarization configurations of dual- interfering beam traps have been investigated...
November 14, 2016: Optics Express
https://www.readbyqxmd.com/read/27842634/a-review-of-the-peri-operative-management-of-paediatric-burns-identifying-adverse-events
#7
H Rode, C Brink, K Bester, M P Coleman, T Baisey, R Martinez
BACKGROUND: Burn injuries are common in poverty-stricken countries. The majority of patients with large and complex burns are referred to burn centres. Of the children who qualify for admission, according to burn admission criteria, about half require some kind of surgical procedure to obtain skin cover. These range from massive full-thickness fire burns to skin grafts for small, residual unhealed wounds. Burn anaesthetic procedures are of the most difficult to perform and are known for high complication rates...
November 2, 2016: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/27829161/sediment-washing-by-edta-and-its-reclamation-by-sodium-polyamidoamine-multi-dithiocarbamate
#8
Tianlin Deng, Bingru Zhang, Fengting Li, Luyao Jin
Sodium polyamidoamine-multi dithiocarbamate (PAMAM-DTC) is a kind of heavy metals capturing agent, containing functional groups of dithiocarbamate that could strongly chelate heavy metals. In this paper, it was applied to precipitate heavy metal ions from meal-EDTA and reclaim EDTA during sediment washing process. The extracting performance of fresh EDTA was studied as a function of EDTA concentration, liquid/sediment (L/S), pH, and extraction time. Then the EDTA effluents were treated with PAMAM-DTC, Na2S and sodium diethyldithiocarbamate (DDTC) to compare their effectiveness on capturing metals from metal-EDTA complexes...
November 6, 2016: Chemosphere
https://www.readbyqxmd.com/read/27823913/-cutaneous-lesions-during-hot-tub-hypersensitivity-pneumonitis-pseudomonas-folliculitis%C3%A2
#9
N Sokolowsky, L Rolland, M-A Vandenhende, J-Y Colin, F Laurent, P Morlat, F Bonnet, M Beylot-Barry
BACKGROUND: Interstitial lung disease, cutaneous rash and elevated serum angiotensin converting enzyme (ACE) may suggest diagnoses other than sarcoidosis. PATIENTS AND METHODS: A 58-year-old man had presented dyspnoea for 2 years with increased angiotensin-converting enzyme, as well as an interstitial syndrome and micronodules. The possibility of sarcoidosis was raised. Systemic corticosteroids resulted in improvement of the dyspnoea although it recurred on dose reduction...
November 4, 2016: Annales de Dermatologie et de Vénéréologie
https://www.readbyqxmd.com/read/27821232/-clinicopathologic-study-of-seromucinous-carcinoma-of-ovary
#10
S X Tang, Y H Sun, Y Xu, X R Zhou, W T Yang
Objective: To elucidate the clinicopathologic characteristics, immunophenotype and differential diagnosis of ovarian seromucinous carcinoma. Methods: The clinical and pathological characteristics of 7 cases of ovarian seromucinous carcinoma were analyzed. Immunohistochemical study of selected markers was performed employing the EnVision method. Literature of ovarian seromucinous carcinoma were also reviewed. Results: The age of the 7 ovarian seromucinous carcinoma patients ranged from 27 to 70 years of age (mean=48 years)...
November 8, 2016: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/27816670/two-barcodes-encoded-by-the-type-1-pdz-and-by-phospho-ser-312-regulate-retromer-wash-mediated-sorting-of-the-%C3%A3-1-adrenergic-receptor-from-endosomes-to-the-plasma-membrane
#11
Mohammed M Nooh, Suleiman W Bahouth
Recycling of the majority of agonist-internalized GPCR is dependent on a type I-PDZ "barcode" in their C-tail. The recycling of wild-type (WT) ß1-AR is also dependent on its default "type-1 PDZ barcode", but trafficking of the ß1-AR is inhibited when PKA or its substrate serine at position 312 (Ser(312)) are inactivated. We tested the hypothesis that phospho-Ser(312) provided a second barcode for ß1-AR sorting from endosomes to the plasma membrane by determining the role of retromer/WASH complexes in ß1-AR trafficking...
November 2, 2016: Cellular Signalling
https://www.readbyqxmd.com/read/27790974/identification-and-reconstitution-of-the-rubber-biosynthetic-machinery-on-rubber-particles-from-hevea-brasiliensis
#12
Satoshi Yamashita, Haruhiko Yamaguchi, Toshiyuki Waki, Yuichi Aoki, Makie Mizuno, Fumihiro Yanbe, Tomoki Ishii, Ayuta Funaki, Yuzuru Tozawa, Yukino Miyagi-Inoue, Kazuhisa Fushihara, Toru Nakayama, Seiji Takahashi
Natural rubber (NR) is stored in latex as rubber particles (RPs), rubber molecules surrounded by a lipid monolayer. Rubber transferase (RTase), the enzyme responsible for NR biosynthesis, is believed to be a member of the cis-prenyltransferase (cPT) family. However, none of the recombinant cPTs have shown RTase activity independently. We show that HRT1, a cPT from Heveabrasiliensis, exhibits distinct RTase activity in vitro only when it is introduced on detergent-washed HeveaRPs (WRPs) by a cell-free translation-coupled system...
October 28, 2016: ELife
https://www.readbyqxmd.com/read/27790833/combining-bleach-and-mild-pre-digestion-improves-ancient-dna-recovery-from-bones
#13
Sanne Boessenkool, Kristian Hanghøj, Heidi M Nistelberger, Clio Der Sarkissian, Agata Gondek, Ludovic Orlando, James H Barrett, Bastiaan Star
The feasibility of genome-scale studies from archaeological material remains critically dependent on the ability to access endogenous, authentic DNA. In the majority of cases, this represents a few percent of the DNA extract, at most. A number of specific pre-extraction protocols for bone powder aimed to improve ancient DNA recovery before library amplification have recently been developed. Here, we test the effects of combining two of such protocols, a bleach wash and a pre-digestion step, on 12 bone samples of Atlantic cod and domestic horse aged 750-1350 cal...
October 28, 2016: Molecular Ecology Resources
https://www.readbyqxmd.com/read/27782812/real-time-pcr-for-the-rapid-identification-and-drug-susceptibility-of-mycobacteria-present-in-bronchial-washings
#14
Thilini Piushani Keerthirathne, Dhammika Nayoma Magana-Arachchi, Dushantha Madegedara, Suneth Sithumini Sooriyapathirana
BACKGROUND: Mycobacteria have a spectrum of virulence and different susceptibilities to antibiotics. Distinguishing mycobacterial species is vital as patients with non-tuberculous mycobacterial (NTM) infections present clinical features that are similar to those of patients with tuberculosis. Thus, rapid differentiation of Mycobacterium tuberculosis complex from NTM is critical to administer appropriate treatment. Hence the aim of the study was to rapid identification of mycobacterial species present in bronchial washings using multiplex real time Polymerase Chain Reaction (PCR) and to determine the drug susceptibility in identified mycobacterial species...
October 26, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27770327/efficacy-of-indigenous-probiotic-lactobacillus-strains-to-reduce-cadmium-bioaccessibility%C3%A2-an-in-vitro-digestion-model
#15
Narendra Kumar, Vijay Kumar, Rohit Panwar, Chand Ram
The toxic heavy metal cadmium (Cd) appears as one of the major global threats to human and animal health. Human being and aquatic life are exposed to Cd by breathing, eating, or drinking when industrial effluents released into environment. The study was aimed to identify cadmium-binding Lactobacillus strain to reduce its bioaccessibility in in vitro digestion model. In this context, forty-eight lactobacilli strains isolated and characterized from fermented dairy products and human origin were screened for their Cd biosorption potential using Flame Atomic Absorption Spectroscopy (FAAS)...
October 21, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27769430/simultaneous-capture-and-sequential-detection-of-two-malarial-biomarkers-on-magnetic-microparticles
#16
Christine F Markwalter, Keersten M Ricks, Anna L Bitting, Lwiindi Mudenda, David W Wright
We have developed a rapid magnetic microparticle-based detection strategy for malarial biomarkers Plasmodium lactate dehydrogenase (pLDH) and Plasmodium falciparum histidine-rich protein II (PfHRPII). In this assay, magnetic particles functionalized with antibodies specific for pLDH and PfHRPII as well as detection antibodies with distinct enzymes for each biomarker are added to parasitized lysed blood samples. Sandwich complexes for pLDH and PfHRPII form on the surface of the magnetic beads, which are washed and sequentially re-suspended in detection enzyme substrate for each antigen...
December 1, 2016: Talanta
https://www.readbyqxmd.com/read/27769407/online-solid-phase-extraction-and-liquid-chromatography-mass-spectrometric-determination-of-nucleoside-drugs-in-plasma
#17
Man Wang, Ping Ma, Xiaonan Xi, Lei Liu, Yabin Wen, Kangning Liu, Liang Sun, Yaxin Lu, Zheng Yin
The bioanalysis and especially the sample preparation of nucleoside drugs in complex media, such as human plasma, has been challenging due to the high polarity and high solubility of these drugs in water. Online solid phase extraction (SPE) offers significant advantages, such as automation and timesaving. Thus, several types of SPE columns have been developed for compounds with different polarities. In this study, SPE was applied to overcome the issue of sample pretreatment of nucleoside drugs in human plasma, with the final aim of establishing a robust analytical platform for drugs with similar structures...
December 1, 2016: Talanta
https://www.readbyqxmd.com/read/27759963/two-3d-isostructural-ln-iii-mofs-displaying-the-slow-magnetic-relaxation-and-luminescence-properties-in-detection-of-nitrobenzene-and-cr2o7-2
#18
Rui-Cheng Gao, Fu-Sheng Guo, Nan-Nan Bai, Yun-Long Wu, Fan Yang, Ji-Ye Liang, Zhen-Jing Li, Yao-Yu Wang
Two new three-dimensional isostructural lanthanide metal-organic frameworks (Ln(III)-MOFs), [LnL(H2O)3]·3H2O·0.75DMF (1-Ln; Ln = Dy(III) and Eu(III) ions, H3L = biphenyl-3'-nitro-3,4',5-tricarboxylic acid, DMF = N,N'-dimethylformamide), were synthesized and characterized. The appearance of temperature-dependent out-of-phase (χ″M) signal reveals that complex 1-Dy displays slow magnetic relaxation behavior with the energy barrier (ΔUeff) of 57 K and a pre-exponential factor (τ0) of 3.89 × 10(-8) s at 1200 Oe direct current field...
October 19, 2016: Inorganic Chemistry
https://www.readbyqxmd.com/read/27757514/a-novel-protocol-for-enzymatic-digestion-based-on-covalent-binding-by-protein-immobilization
#19
Qian Qi, Guoquan Yan, Chunhui Deng, Xiangmin Zhang
The process of protein digestion is a critical step for successful protein identification in proteomic analysis. Many efforts have been dedicated to enhancing the digestion efficiency for sufficient digestion. Among these approaches, protein complete denaturation with denaturants is a common process for better digestion. However, the removal of denaturants was tedious or would cause protein loss and other problems. In this work, a feasible digestion approach, immobilized protein digestion (IPD), based on covalent binding has been developed...
November 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27734602/recurrent-heparin-induced-thrombocytopenia-due-to-heparin-rinsing-before-priming-the-machine-in-a-hemodialysis-patient-a-case-report
#20
Jin Han Lim, Kyung Pyo Kang, Sik Lee, Sung Kwang Park, Won Kim
Heparin has remained the most commonly used anticoagulant for patients undergoing hemodialysis. It is usually safe to use but can have severe adverse effects in some cases. Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of exposure to heparin. It results from an autoantibody directed against endogenous platelet factor 4 (PF4) in complex with heparin, which activates platelets and can cause catastrophic arterial and venous thromboses. Here, we present the case of an 80-year-old woman with a recent diagnosis of chronic renal failure who developed acute HIT (platelet count nadir, 15 × 10(9) /L) on day 7 of hemodialysis performed with routine heparin anticoagulation, who despite subsequent heparin-free hemodialysis (with argatroban and warfarin) developed recurrent HIT (complicated by acute cerebral infarction) on day 11 that we attributed to "rinsing" of the circuit with heparin-containing saline (3,000 units of unfractionated heparin, with subsequent saline washing) performed pre-dialysis as per routine...
October 13, 2016: Hemodialysis International
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