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https://www.readbyqxmd.com/read/28336842/sers-based-flavonoid-detection-using-ethylenediamine-%C3%AE-cyclodextrin-as-a-capturing-ligand
#1
Jae Min Choi, Eunil Hahm, Kyeonghui Park, Daham Jeong, Won-Yeop Rho, Jaehi Kim, Dae Hong Jeong, Yoon-Sik Lee, Sung Ho Jhang, Hyun Jong Chung, Eunae Cho, Jae-Hyuk Yu, Bong-Hyun Jun, Seunho Jung
Ethylenediamine-modified β-cyclodextrin (Et-β-CD) was immobilized on aggregated silver nanoparticle (NP)-embedded silica NPs (SiO₂@Ag@Et-β-CD NPs) for the effective detection of flavonoids. Silica NPs were used as the template for embedding silver NPs to create hot spots and enhance surface-enhanced Raman scattering (SERS) signals. Et-β-CD was immobilized on Ag NPs to capture flavonoids via host-guest inclusion complex formation, as indicated by enhanced ultraviolet absorption spectra. The resulting SiO₂@Ag@Et-β-CD NPs were used as the SERS substrate for detecting flavonoids, such as hesperetin, naringenin, quercetin, and luteolin...
January 6, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28335974/selective-solid-phase-extraction-of-lanthanides-from-tap-and-river-waters-with-ion-imprinted-polymers
#2
Manel Moussa, Massamba Mbacké Ndiaye, Thomas Pinta, Valérie Pichon, Thomas Vercouter, Nathalie Delaunay
For the first time, an ion imprinted polymer (IIP) able to selectively extract simultaneously all the lanthanide ions was successfully synthesized in acetonitrile using Nd(3+) as a template ion, methacrylic acid as a complexing monomer, and ethylene glycol dimethacrylate as a cross-linker. A non-imprinted polymer (NIP) was synthesized under the same conditions as those of the IIP, but in the absence of the template ion. After the removal of the template ions, grounding and sieving, the IIP particles were packed in solid phase extraction (SPE) cartridges...
April 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28303638/a-review-of-the-methods-for-storing-floral-nectars-in-the-field
#3
REVIEW
B Amato, S Petit
The knowledge of floral nectar sugar characteristics such as concentration, ratio, and mass is essential to understand the complex nature of nectar production and pollination systems. Although nectar is commonly stored in ecology, storage reliability and effectiveness have rarely been quantified. Inappropriate nectar storage between sampling and analysis can alter nectar chemistry as a result of enzyme or microbial action. Our review of the literature indicates that measures to preserve nectar sugars before analysis include refrigeration, freezing, the addition of an antimicrobial agent, spotting and drying on filter paper, the addition of a desiccant, or a combination of these storage treatments...
March 17, 2017: Plant Biology
https://www.readbyqxmd.com/read/28287572/rapid-mix-preparation-of-bioinspired-nanoscale-hydroxyapatite-for-biomedical-applications
#4
Caroline J Wilcock, Piergiorgio Gentile, Paul V Hatton, Cheryl A Miller
Hydroxyapatite (HA) has been widely used as a medical ceramic due to its good biocompatibility and osteoconductivity. Recently there has been interest regarding the use of bioinspired nanoscale hydroxyapatite (nHA). However, biological apatite is known to be calcium-deficient and carbonate-substituted with a nanoscale platelet-like morphology. Bioinspired nHA has the potential to stimulate optimal bone tissue regeneration due to its similarity to bone and tooth enamel mineral. Many of the methods currently used to fabricate nHA both in the laboratory and commercially, involve lengthy processes and complex equipment...
February 23, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28257572/bead-extractor-assisted-ready-to-use-reagent-system-bears-for-immunoprecipitation-coupled-to-maldi-ms
#5
Huiyan Li, Robert Popp, Michael Chen, Elizabeth M MacNamara, David Juncker, Christoph H Borchers
Quantitative protein assays play an important role in the study of biological functions. Immunoassays and mass spectrometry are two main technologies for quantifying proteins in biological samples. The combination of immunoprecipitation (IP) with MALDI technology delivers high assay sensitivity and specificity, but the sample preparation procedure involves multiple washing and transfer steps. These steps can be performed either manually (requiring significant time and labor) or automatically (requiring the purchase of a complex liquid-handling workstation)...
March 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28256723/immunoblotting-and-immunodetection
#6
Duojiao Ni, Peng Xu, Sean Gallagher
Immunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of SDS and reducing agents. Following solubilization, the material is separated by SDS-PAGE and the antigens are electrophoretically transferred to a membrane, a process that can be monitored by reversible staining with Ponceau S. The transferred proteins are bound to the surface of the membrane, providing access to immunodetection reagents...
March 3, 2017: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/28245111/simplifying-nucleic-acid-amplification-from-whole-blood-with-direct-polymerase-chain-reaction-on-chitosan-microparticles
#7
Imaly A Nanayakkara, Weidong Cao, Ian M White
Tremendous advances have been made in the development of portable nucleic acid amplification devices for near-patient use. However, the true limitation in the realization of nucleic acid amplification tests (NAATs) for near-patient applications is not the amplification reaction, it is the complexity of the sample preparation. Conventional approaches require several precise intervention steps during the protocol. There are numerous reports in the literature that mimic the sample preparation procedure within a lab-on-a-chip device or cartridge, but these systems require a high number of integrated steps, making the devices and/or their supporting equipment too complex to meet the necessary cost targets and regulatory requirements for near-patient applications...
March 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28245105/machine-washable-pedot-pss-dyed-silk-yarns-for-electronic-textiles
#8
Jason D Ryan, Desalegn Alemu Mengistie, Roger Gabrielsson, Anja Lund, Christian Müller
Durable, electrically conducting yarns are a critical component of electronic textiles (e-textiles). Here, such yarns with exceptional wear and wash resistance are realized through dyeing silk from the silkworm Bombyx mori with the conjugated polymer:polyelectrolyte complex PEDOT:PSS. A high Young's modulus of approximately 2 GPa combined with a robust and scalable dyeing process results in up to 40 m long yarns that maintain their bulk electrical conductivity of approximately 14 S cm(-1) when experiencing repeated bending stress as well as mechanical wear during sewing...
March 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28238724/proximity-biotinylation-as-a-method-for-mapping-proteins-associated-with-mtdna-in-living-cells
#9
Shuo Han, Namrata D Udeshi, Thomas J Deerinck, Tanya Svinkina, Mark H Ellisman, Steven A Carr, Alice Y Ting
A recurring challenge in cell biology is to define the molecular components of macromolecular complexes of interest. The predominant method, immunoprecipitation, recovers only strong interaction partners that survive cell lysis and repeated detergent washes. We explored peroxidase-catalyzed proximity biotinylation, APEX, as an alternative, focusing on the mitochondrial nucleoid, the dynamic macromolecular complex that houses the mitochondrial genome. Via 1-min live-cell biotinylation followed by quantitative, ratiometric mass spectrometry, we enriched 37 nucleoid proteins, seven of which had never previously been associated with the nucleoid...
March 16, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28235460/tyrozine-d-oxidation-and-redox-equilibrium-in-photosystem-ii
#10
Nigar Ahmadova, Felix Ho, Stenbjorn Styring, Fikret Mamedov
Tyrosine D (TyrD) is an auxiliary redox active tyrosine residue in photosystem II (PsiI). The mechanism of TyrD oxidation was investigated by EPR spectroscopy, flash-induced fluorescence decay and thermoluminescence measurements in PsiI enriched membranes from spinach. PsiI membranes were chemically treated with 3mM ascorbate and 1mM diaminodurene and subsequent washing, leading to the complete reduction of TyrD. TyrD oxidation kinetics and competing recombination reactions were measured after a single saturating flash in the absence and presence of DCMU (inhibitor of the QB-site) in the pH range of 4...
February 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28215401/nickel-salen-supported-paramagnetic-nanoparticles-for-6-his-target-recombinant-protein-affinity-purification
#11
Zahra Rashid, Ramin Ghahremanzadeh, Mohammad-Reza Nejadmoghaddam, Mahboobeh Nazari, Mohammad-Reza Shokri, Hossein Naeimi, Amir-Hassan Zarnani
In this research, a simple, efficient, inexpensive, rapid and high yield method for the purification of 6×histidine-tagged recombinant protein was developed. For this purpose, manganese ferrite magnetic nanoparticles (MNPs) were synthesized through a co-precipitation method and then they were conveniently surface-modified with tetraethyl orthosilicate (TEOS) in order to prevent oxidation and form high density of hydroxyl groups. Next, the salen ligand was prepared from condensation reaction of salicylaldehyde and 3-aminopropyl (trimethoxy) silane (APTMS) in 1:1 molar ratio; followed by complexation with Ni(OAc)2...
February 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28211040/anti-cd3-treatment-up-regulates-programmed-cell-death-protein-1-expression-on-activated-effector-t-cells-and-severely-impairs-their-inflammatory-capacity
#12
Maja Wallberg, Asha Recino, Jenny Phillips, Duncan Howie, Margaux Vienne, Christopher Paluch, Miyuki Azuma, F Susan Wong, Herman Waldmann, Anne Cooke
T cells play a key role in the pathogenesis of type 1 diabetes, and targeting the CD3 component of the T-cell receptor complex provides one therapeutic approach. Anti-CD3 treatment can reverse overt disease in spontaneously diabetic non-obese diabetic mice, an effect proposed to, at least in part, be caused by a selective depletion of pathogenic cells. We have used a transfer model to further investigate the effects of anti-CD3 treatment on green fluorescent protein (GFP)(+) islet-specific effector T cells in vivo...
February 17, 2017: Immunology
https://www.readbyqxmd.com/read/28206771/magnetic-particle-imaging-a-novel-in-vivo-imaging-platform-for-cancer-detection
#13
Elaine Y Yu, Mindy Bishop, Bo Zheng, R Matthew Ferguson, Amit P Khandhar, Scott J Kemp, Kannan M Krishnan, Patrick W Goodwill, Steven M Conolly
Cancer remains one of the leading causes of death worldwide. Biomedical imaging plays a crucial role in all phases of cancer management. Physicians often need to choose the ideal diagnostic imaging modality for each clinical presentation based on complex trade-offs among spatial resolution, sensitivity, contrast, access, cost, and safety. Magnetic particle imaging (MPI) is an emerging tracer imaging modality that detects superparamagnetic iron oxide (SPIO) nanoparticle tracer with high image contrast (zero tissue background signal), high sensitivity (200 nM Fe) with linear quantitation, and zero signal depth attenuation...
March 8, 2017: Nano Letters
https://www.readbyqxmd.com/read/28194258/complete-genome-sequence-of-pseudomonas-stutzeri-strain-rch2-isolated-from-a-hexavalent-chromium-cr-vi-contaminated-site
#14
Romy Chakraborty, Hannah Woo, Paramvir Dehal, Robert Walker, Marcin Zemla, Manfred Auer, Lynne A Goodwin, Alexey Kazakov, Pavel Novichkov, Adam P Arkin, Terry C Hazen
Hexavalent Chromium [Cr(VI)] is a widespread contaminant found in soil, sediment, and ground water in several DOE sites, including Hanford 100 H area. In order to stimulate microbially mediated reduction of Cr(VI) at this site, a poly-lactate hydrogen release compound was injected into the chromium contaminated aquifer. Targeted enrichment of dominant nitrate-reducing bacteria post injection resulted in the isolation of Pseudomonas stutzeri strain RCH2. P. stutzeri strain RCH2 was isolated using acetate as the electron donor and is a complete denitrifier...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28193039/molecularly-imprinted-polymer-based-plasmonic-immunosandwich-assay-for-fast-and-ultrasensitive-determination-of-trace-glycoproteins-in-complex-samples
#15
Xueying Tu, Pir Muhammad, Jia Liu, Yanyan Ma, Shuangshou Wang, Danyang Yin, Zhen Liu
Glycoproteins play significant roles in many biological processes. Assays of glycoproteins have significant biological importance and clinical values, for which immunoassay has been the workhorse tool. However, immunoassay suffers from some disadvantages, such as poor availability of high-specificity antibodies and limited stability of biological reagents. Herein, we present an antibody-free and enzyme-free approach, called molecularly imprinted polymer (MIP)-based plasmonic immunosandwich assay (PISA), for fast and ultrasensitive detection of trace glycoproteins in complex samples...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28191268/automated-electrotransformation-of-escherichia-coli-on-a-digital-microfluidic-platform-using-bioactivated-magnetic-beads
#16
J A Moore, M Nemat-Gorgani, A C Madison, M A Sandahl, S Punnamaraju, A E Eckhardt, M G Pollack, F Vigneault, G M Church, R B Fair, M A Horowitz, P B Griffin
This paper reports on the use of a digital microfluidic platform to perform multiplex automated genetic engineering (MAGE) cycles on droplets containing Escherichia coli cells. Bioactivated magnetic beads were employed for cell binding, washing, and media exchange in the preparation of electrocompetent cells in the electrowetting-on-dieletric (EWoD) platform. On-cartridge electroporation was used to deliver oligonucleotides into the cells. In addition to the optimization of a magnetic bead-based benchtop protocol for generating and transforming electrocompetent E...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28176202/bioethanol-production-from-steam-exploded-rice-husk-by-recombinant-escherichia-coli-ko11
#17
Takamitsu Tabata, Yusuke Yoshiba, Tomonori Takashina, Kazuo Hieda, Norio Shimizu
Rice husk is one of the most abundant types of lignocellulosic biomass. Because of its significant amount of sugars, such as cellulose and hemicellulose, it can be used for the production of biofuels such as bioethanol. However, the complex structure of lignocellulosic biomass, consisting of cellulose, hemicellulose and lignin, is resistant to degradation, which limits biomass utilization for ethanol production. The protection of cellulose by lignin contributes to the recalcitrance of lignocelluloses to hydrolysis...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28132175/collection-and-preparation-of-clinical-samples-for-metabolomics
#18
Andrew J Chetwynd, Warwick B Dunn, Giovanny Rodriguez-Blanco
A wide range of biofluids (urine, serum, plasma, saliva, etc.) as well as cellular and tissue samples can be collected and investigated in clinical metabolomic studies. The choice of sample is dependent on the clinical question being investigated with biofluids typically studied to identify biomarkers, whereas tissues and primary/immortalised cells are typically studied to investigate mechanisms associated with pathophysiological processes. Methods applied to collect samples, quench metabolism and extract samples differ between sample types from simple collect, dilute and analyse methods for urine to complex washing, quenching and biphasic extraction methods for tissues...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28126601/rapid-screening-for-mycobacterium-tuberculosis-complex-in-clinical-elephant-trunk-wash-samples
#19
Roberta J Magnuson, Lyndsey M Linke, Ramiro Isaza, Mo D Salman
Mycobacterium tuberculosis can infect and be transmitted between elephants and humans. In elephants, the 'gold standard' reference test for detection of tuberculosis is culture, which takes a minimum of eight weeks for results and has limited sensitivity. A screening test that is rapid, easily implemented, and accurate is needed to aid in diagnosis of tuberculosis in elephants. Ninety-nine clinical trunk wash samples obtained from 33 elephants were utilized to validate three molecular extraction techniques followed by a polymerase chain reaction for detection of M...
January 3, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28125625/on-the-identification-of-associations-between-five-world-health-organization-water-sanitation-and-hygiene-phenotypes-and-six-predictors-in-low-and-middle-income-countries
#20
Hugh Ellis, Erica Schoenberger
BACKGROUND: According to the most recent estimates, 842,000 deaths in low- to middle-income countries were attributable to inadequate water, sanitation and hygiene in 2012. Despite billions of dollars and decades of effort, we still lack a sound understanding of which kinds of WASH interventions are most effective in improving public health outcomes, and an important corollary-whether the right things are being measured. The World Health Organization (WHO) has made a concerted effort to compile comprehensive data on drinking water quality and sanitation in the developing world...
2017: PloS One
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