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https://www.readbyqxmd.com/read/29149905/the-kusamala-program-for-primary-caregivers-of-children-6-59-months-of-age-hospitalized-with-severe-acute-malnutrition-in-malawi-study-protocol-for-a-cluster-randomized-controlled-trial
#1
Allison I Daniel, Meta van den Heuvel, Wieger P Voskuijl, Melissa Gladstone, Mike Bwanali, Isabel Potani, Celine Bourdon, Jenala Njirammadzi, Robert H J Bandsma
BACKGROUND: Severe acute malnutrition (SAM) is associated with high mortality rates and impairments in growth and development in children that do survive. There are complex nutritional, health, and behavioural risk factors involving severely malnourished children and their primary caregivers, requiring integrated intervention approaches. METHODS: A cluster-randomized controlled trial at the Queen Elizabeth Central Hospital in Blantyre, Malawi will be conducted to evaluate the effectiveness of a 4-day hospital-based intervention programme directed at primary caregivers...
November 17, 2017: Trials
https://www.readbyqxmd.com/read/29143662/heat-treatment-and-false-positive-heartworm-antigen-testing-in-ex-vivo-parasites-and-dogs-naturally-infected-by-dirofilaria-repens-and-angiostrongylus-vasorum
#2
Luigi Venco, Simone Manzocchi, Marco Genchi, Laura H Kramer
BACKGROUND: Heartworm antigen testing is considered sensitive and specific. Currently available tests are reported as detecting a glycoprotein found predominantly in the reproductive tract of the female worm and can reach specificity close to 100%. Main concerns regard sensitivity in the case of light infections, the presence of immature females or cases of all-male infections. Research and development have been aimed at increasing sensitivity. Recently, heat treatment of serum prior to antigen testing has been shown to result in an increase in positive antigen test results, presumably due to disruption of natural antigen-antibody complexes...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29143215/combining-cationic-and-anionic-mixed-mode-sorbents-in-a-single-cartridge-to-extract-basic-and-acidic-pharmaceuticals-simultaneously-from-environmental-waters
#3
Daniela Salas, Francesc Borrull, Núria Fontanals, Rosa Maria Marcé
The aim of the present study is to broaden the applications of mixed-mode ion-exchange solid-phase extraction sorbents to extract both basic and acidic compounds simultaneously by combining the sorbents in a single cartridge and developing a simplified extraction procedure. Four different cartridges containing negative and positive charges in the same configuration were evaluated and compared to extract a group of basic, neutral, and acidic pharmaceuticals selected as model compounds. After a thorough optimization of the extraction conditions, the four different cartridges showed to be capable of retaining basic and acidic pharmaceuticals simultaneously through ionic interactions, allowing the introduction of a washing step with 15 mL methanol to eliminate interferences retained by hydrophobic interactions...
November 15, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29143065/-complex-joint-reconstruction-and-joint-transplantation-with-the-flocsat-concept-planning-and-surgical-implementation
#4
REVIEW
C Krettek, J-D Clausen, C Neunaber
Cartilage defects in adult patients are so far incurable. Fresh osteochondral allograft (OCA) transplantation is based on the insertion of mature, living, mechanically sound hyaline cartilage into an osteochondral defect where it undergoes osseointegration. Intact hyaline cartilage of OCA does not cause immune reactions in the recipient. Many reports show that small OCA have good osseointegration and show good long-term results. These observations have been incorporated into the development of the fresh large (> 10 cm(2)) osteochondral shell allograft transplantation (FLOCSAT) concept, which is based on the following principles: 1) the thickness of the osseous layer should be kept as thin as possible (target < 6-8 mm) so that the transplant remains stable and fixable...
November 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/29136919/flow-batch-analysis-of-clenbuterol-based-on-analyte-extraction-on-molecularly-imprinted-polymers-coupled-to-an-in-system-chromogenic-reaction-application-to-human-urine-and-milk-substitute-samples
#5
Natalia González, Marcos Grünhut, Ivana Šrámková, Adriana G Lista, Burkhard Horstkotte, Petr Solich, Hana Sklenářová, Carolina C Acebal
A fully automated spectrophotometric method based on flow-batch analysis has been developed for the determination of clenbuterol including an on-line solid phase extraction using a molecularly imprinted polymer (MIP) as the sorbent. The molecularly imprinted solid phase extraction (MISPE) procedure allowed analyte extraction from complex matrices at low concentration levels and with high selectivity towards the analyte. The MISPE procedure was performed using a commercial MIP cartridge that was introduced into a guard column holder and integrated in the analyzer system...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136898/aerosol-dilution-as-a-simple-strategy-for-analysis-of-complex-samples-by-icp-ms
#6
Ariane I Barros, Fernanda C Pinheiro, Clarice D B Amaral, Rodolfo Lorençatto, Joaquim A Nóbrega
This study investigated the capability of High Matrix Introduction (HMI) strategy for analysis of dialysis solution and urine samples using inductively coupled plasma mass spectrometry. The use of HMI enables the direct introduction of urine samples and dialysis solutions 2-fold diluted with 0.14molL(-1) HNO3. Bismuth, Ge, Ir, Li, Pt, Rh, Sc and Tl were evaluated as internal standards for Al, Ag, As, Be, Cd, Cr, Pb, Sb, Se, Tl, and Hg determination in dialysis solution and As, Cd, Hg and Pb determination in urine samples...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29128833/structurally-stable-n-t-butylacrylamide-hydrogel-particles-for-the-capture-of-peptides
#7
Megan Devine, Melanie Juba, Paul Russo, Barney Bishop
Hydrogel particles have proven to be powerful tools for the capture and concentration of low abundance, low molecular weight peptides and proteins from complex biofluids, such as plasma. The primary means of recovering and washing the particles following harvesting is through centrifugation, which can be a very time-consuming process depending on harvest conditions. To improve the process of particle recovery, washing, and elution we have developed new particle formulations: incorporating N-t-butylacrylamide (tBA) in the polymer backbone with monomers bearing more acidic functional groups and higher degrees of cross-linking...
November 2, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29126479/fast-probing-of-glucose-and-fructose-in-plant-tissues-via-plasmonic-affinity-sandwich-assay-with-molecularly-imprinted-extraction-microprobes
#8
Pir Muhammad, Jia Liu, Rongrong Xing, Yanrong Wen, Yijia Wang, Zhen Liu
Determination of specific target compounds in agriculture food and natural plant products is essential for many purposes; however, it is often challenging due to the complexity of the sample matrices. Herein we present a new approach called plasmonic affinity sandwich assay for the facile and rapid probing of glucose and fructose in plant tissues. The approach mainly relies on molecularly imprinted plasmonic extraction microprobes, which were prepared on gold-coated acupuncture needles via boronate affinity controllable oriented surface imprinting with the target monosaccharide as the template molecules...
December 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29095441/identification-of-rna-binding-domains-of-rna-binding-proteins-in-cultured-cells-on-a-system-wide-scale-with-rbdmap
#9
Alfredo Castello, Christian K Frese, Bernd Fischer, Aino I Järvelin, Rastislav Horos, Anne-Marie Alleaume, Sophia Foehr, Tomaz Curk, Jeroen Krijgsveld, Matthias W Hentze
This protocol is an extension to: Nat. Protoc. 8, 491-500 (2013); doi:10.1038/nprot.2013.020; published online 14 February 2013RBDmap is a method for identifying, in a proteome-wide manner, the regions of RNA-binding proteins (RBPs) engaged in native interactions with RNA. In brief, cells are irradiated with UV light to induce protein-RNA cross-links. Following stringent denaturing washes, the resulting covalently linked protein-RNA complexes are purified with oligo(dT) magnetic beads. After elution, RBPs are subjected to partial proteolysis, in which the protein regions still bound to the RNA and those released to the supernatant are separated by a second oligo(dT) selection...
December 2017: Nature Protocols
https://www.readbyqxmd.com/read/29089495/fully-inkjet-printed-two-dimensional-material-field-effect-heterojunctions-for-wearable-and-textile-electronics
#10
Tian Carey, Stefania Cacovich, Giorgio Divitini, Jiesheng Ren, Aida Mansouri, Jong M Kim, Chaoxia Wang, Caterina Ducati, Roman Sordan, Felice Torrisi
Fully printed wearable electronics based on two-dimensional (2D) material heterojunction structures also known as heterostructures, such as field-effect transistors, require robust and reproducible printed multi-layer stacks consisting of active channel, dielectric and conductive contact layers. Solution processing of graphite and other layered materials provides low-cost inks enabling printed electronic devices, for example by inkjet printing. However, the limited quality of the 2D-material inks, the complexity of the layered arrangement, and the lack of a dielectric 2D-material ink able to operate at room temperature, under strain and after several washing cycles has impeded the fabrication of electronic devices on textile with fully printed 2D heterostructures...
October 31, 2017: Nature Communications
https://www.readbyqxmd.com/read/29084197/tbc1d23-is-a-bridging-factor-for-endosomal-vesicle-capture-by-golgins-at-the-trans-golgi
#11
John J H Shin, Alison K Gillingham, Farida Begum, Jessica Chadwick, Sean Munro
The specificity of membrane traffic involves tethers at destination organelles that selectively capture incoming transport vesicles to allow SNAREs on opposing membranes to then assemble and drive fusion. Tethers include both protein complexes and long coiled-coil proteins, although how they contribute to specificity remains unclear. The golgin coiled-coil proteins at the Golgi apparatus capture vesicles from different origins, but the vesicle-specific molecular cues that they recognize are unknown. Vesicle tethering is typically a transient process and therefore is challenging to interrogate in vivo...
October 30, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/29081948/fluorescent-turn-on-probes-for-wash-free-mrna-imaging-via-covalent-site-specific-enzymatic-labeling
#12
Cun Yu Zhou, Seth C Alexander, Neal K Devaraj
Investigating the many roles RNA plays in cellular regulation and function has increased demand for tools to explore RNA tracking and localization within cells. Our recently reported RNA-TAG (transglycosylation at guanine) approach uses an RNA-modifying enzyme, tRNA-guanine transglycosylase (TGT), to accomplish covalent labeling of an RNA of interest with fluorescent tracking agents in a highly selective and efficient manner. Unfortunately, labeling by this method currently suffers from a high nonspecific fluorescent background and is currently unsuitable for imaging RNA within complex cellular environments...
October 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/29073356/microfluidic-system-for-detection-of-viral-rna-in-blood-using-a-barcode-fluorescence-reporter-and-a-photocleavable-capture-probe
#13
Ke Du, Myeongkee Park, Anthony Griffiths, Ricardo Carrion, Jean Patterson, Holger Schmidt, Richard Mathies
A microfluidic sample preparation multiplexer (SPM) and assay procedure is developed to improve amplification-free detection of Ebola virus RNA from blood. While a previous prototype successfully detected viral RNA following off-chip RNA extraction from infected cells, the new device and protocol can detect Ebola virus in raw blood with clinically relevant sensitivity. The Ebola RNA is hybridized with sequence specific capture and labeling DNA probes in solution and then the complex is pulled down onto capture beads for purification and concentration...
November 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29070863/parp1-produced-poly-adp-ribose-causes-the-parp12-translocation-to-stress-granules-and-impairment-of-golgi-complex-functions
#14
Giuliana Catara, Giovanna Grimaldi, Laura Schembri, Daniela Spano, Gabriele Turacchio, Matteo Lo Monte, Andrea Rosario Beccari, Carmen Valente, Daniela Corda
Poly-ADP-ribose-polymerases (PARPs) 1 and 2 are nuclear enzymes that catalyze the poly-ADP-ribosylation of nuclear proteins transferring poly-ADP-ribose (PAR) polymers to specific residues. PARPs and PAR intervene in diverse functions, including DNA repair in the nucleus and stress granule assembly in the cytoplasm. Stress granules contribute to the regulation of translation by clustering and stabilizing mRNAs as well as several cytosolic PARPs and signaling proteins to modulate cell metabolism and survival...
October 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29055527/multi-state-steric-mass-action-model-and-case-study-on-complex-high-loading-behavior-of-mab-on-ion-exchange-tentacle-resin
#15
Juliane Diedrich, William Heymann, Samuel Leweke, Stephen Hunt, Robert Todd, Christian Kunert, Will Johnson, Eric von Lieres
Tentacle resins for IEX are increasingly applied in preparative chromatography for their higher selectivity and higher capacities in comparison to IEX resins without tentacles. However, tentacle resins are often observed to cause unusual elution behavior of monoclonal antibodies under high loading conditions. Understanding this elution behavior is important for a quality by design approach, as it is now mandated by regulatory agencies. A model-based analysis of load, wash and gradient elution is performed for a monoclonal antibody (mAb) on Fractogel SO3(-)...
September 20, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29033838/the-target-residence-time-of-antihistamines-determines-their-antagonism-of-the-g-protein-coupled-histamine-h1-receptor
#16
Reggie Bosma, Gesa Witt, Lea A I Vaas, Ivana Josimovic, Philip Gribbon, Henry F Vischer, Sheraz Gul, Rob Leurs
The pharmacodynamics of drug-candidates is often optimized by metrics that describe target binding (Kd or Ki value) or target modulation (IC50). However, these metrics are determined at equilibrium conditions, and consequently information regarding the onset and offset of target engagement and modulation is lost. Drug-target residence time is a measure for the lifetime of the drug-target complex, which has recently been receiving considerable interest, as target residence time is shown to have prognostic value for the in vivo efficacy of several drugs...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29030663/extraction-of-micrornas-from-biological-matrices-with-titanium-dioxide-nanofibers
#17
Luis A Jimenez, Marissa A Gionet-Gonzales, Sabrina Sedano, Jocelyn G Carballo, Yomara Mendez, Wenwan Zhong
MicroRNAs (miRNAs) are small RNAs that bind to mRNA targets and regulate their translation. A functional study of miRNAs and exploration of their utility as disease markers require miRNA extraction from biological samples, which contain large amounts of interfering compounds for downstream RNA identification and quantification. The most common extraction methods employ silica columns or the TRIzol reagent but give out low recovery for small RNAs probably due to their short strand lengths. Herein, we fabricated the titanium dioxide nanofibers using electrospinning to facilitate miRNA extraction and developed the optimal buffer conditions to improve miRNA recovery from biological matrices of cell lysate and serum...
October 13, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28990973/the-issue-of-gadolinium-retained-in-tissues-insights-on-the-role-of-metal-complex-stability-by-comparing-metal-uptake-in-murine-tissues-upon-the-concomitant-administration-of-lanthanum-and-gadolinium-diethylentriamminopentaacetate
#18
Enza Di Gregorio, Giuseppe Ferrauto, Chiara Furlan, Stefania Lanzardo, Raffaele Nuzzi, Eliana Gianolio, Silvio Aime
OBJECTIVES: The aim of the study was to explore the role of the stability of metal complexes in the processes that lead to the metal retention in the brain and other tissues of mice administered with lanthanides-based contrast agents. This issue was tackled by the simultaneous injection of gadolinium (Gd)-diethylentriamminopentaacetate (DTPA) and lanthanum-DTPA, which have the same charge and structure but differ in their thermodynamic stability by 3 orders of magnitude. MATERIALS AND METHODS: A total of 20 healthy BALB/c mice were administered by a single intravenous injection with a dose consisting of 0...
October 3, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28986999/one-step-affinity-capture-and-precipitation-for-improved-purification-of-an-industrial-monoclonal-antibody-using-z-elp-functionalized-nanocages
#19
Andrew R Swartz, Xuankuo Xu, Steven J Traylor, Zheng J Li, Wilfred Chen
Protein A chromatography has been identified as a potential bottleneck in the monoclonal antibody production platform, leading to increased interest in non-chromatographic capture technologies. Affinity precipitation using environmentally responsive, Z-domain-elastin-like polypeptide (Z-ELP) fusion proteins has been shown to be a promising alternative. However, elevated temperature and salt concentrations necessary for precipitation resulted in decreased antibody monomer content and reduced purification capacity...
October 7, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28973448/targeted-chromatin-ligation-a-robust-epigenetic-profiling-technique-for-small-cell-numbers
#20
Mark A Zarnegar, Felicia Reinitz, Aaron M Newman, Michael F Clarke
The complexity and inefficiency of chromatin immunoprecipitation strategies restrict their sensitivity and application when examining rare cell populations. We developed a new technique that replaces immunoprecipitation with a simplified chromatin fragmentation and proximity ligation step that eliminates bead purification and washing steps. We present a simple single tube proximity ligation technique, targeted chromatin ligation, that captures histone modification patterns with only 200 cells. Our technique eliminates loss of material and sensitivity due to multiple inefficient steps, while simplifying the workflow to enhance sensitivity and create the potential for novel applications...
September 29, 2017: Nucleic Acids Research
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