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https://www.readbyqxmd.com/read/28704876/mesenteric-masses-on-2d-mode-and-contrast-enhanced-ultrasound-a-retrospective-study-in-69-patients
#1
Corinna Trenker, Ehsan Safaei Zadeh, Golo Petzold, Christian Görg, Albrecht Neesse
Purpose Detection of mesenteric masses (MM) by 2 D ultrasound often causes a diagnostic dilemma, and histological confirmation is required for definite diagnosis. The value of contrast-enhanced ultrasound (CEUS) for the diagnosis of MMs has not been investigated before. Here, we retrospectively evaluate 2D-mode and CEUS patterns of 69 patients with histologically confirmed MMs. Materials and Methods Between January 2006 and January 2016, n = 69 patients were included in the study. Histopathological data, clinical data, 2D-mode ultrasound and CEUS enhancement were retrospectively analyzed...
July 13, 2017: Ultraschall in der Medizin
https://www.readbyqxmd.com/read/28697458/uptake-and-toxicity-of-cuo-nanoparticles-to-daphnia-magna-varies-between-indirect-dietary-and-direct-waterborne-exposures
#2
Fan Wu, Amy Bortvedt, Bryan J Harper, Lauren E Crandon, Stacey L Harper
Research examining the direct and indirect ecological effects of nanomaterials in aquatic ecosystems is important for developing a more realistic understanding of the environmental implications of nanotechnology. Copper oxide nanoparticles (CuO NPs) are being used extensively in many industries but are considered highly toxic to aquatic species residing in surface waters. Few studies have addressed whether CuO NPs can be transferred through the aquatic food chain, and if such indirect exposure to nanomaterials impacts their toxicity...
June 26, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28685328/generation-of-domestic-waste-electrical-and-electronic-equipment-on-fernando-de-noronha-island-qualitative-and-quantitative-aspects
#3
Dhiego Raphael Rodrigues Araujo, José Diego de Oliveira, Vanice Fragoso Selva, Maisa Mendonça Silva, Simone Machado Santos
The accelerated growth trajectory of waste electrical and electronic equipment (WEEE) is a matter of concern for governments worldwide. In developing countries, the problem is more complex because municipal waste management is still a challenge for municipalities. Fernando de Noronha Island, an environmentally protected area, has a transfer station for solid waste before it is sent to the final destination abroad, which is different waste management model to most urban areas. In order to check the specifics of management of WEEE, this study aimed to qualitatively and quantitatively evaluate the generation of this type of waste on the main island of Fernando de Noronha, taking into consideration aspects related to consumption habits and handling of waste...
July 6, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28679706/indirect-competitive-enzyme-linked-immunosorbent-assay-elisa
#4
Thomas O Kohl, Carl A Ascoli
The indirect competitive ELISA (indirect cELISA) pits plate-immobilized antigen against antigens in solution for binding to antigen-specific antibody. The antigens in solution are in the test sample and are first incubated with antigen-specific antibody. These antibody-antigen complexes are then added to microtiter plates whose wells have been coated with purified antigen. The wells are washed to remove unbound antigen-antibody complexes and free antigen. A reporter-labeled secondary antibody is then added followed by the addition of substrate...
July 5, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28679705/direct-competitive-enzyme-linked-immunosorbent-assay-elisa
#5
Thomas O Kohl, Carl A Ascoli
The competitive enzyme-linked immunosorbent assay (ELISA) (cELISA; also called an inhibition ELISA) is designed so that purified antigen competes with antigen in the test sample for binding to an antibody that has been immobilized in microtiter plate wells. The same concept works if the immobilized molecule is antigen and the competing molecules are purified labeled antibody versus antibody in a test sample. Direct cELISAs incorporate labeled antigen or antibody, whereas indirect assay configurations use reporter-labeled secondary antibodies...
July 5, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28672835/magnetic-solid-phase-extraction-with-fe%C3%A2-o%C3%A2-molecularly-imprinted-polymers-modified-by-deep-eutectic-solvents-and-ionic-liquids-for-the-rapid-purification-of-alkaloid-isomers-theobromine-and-theophylline-from-green-tea
#6
Guizhen Li, Xiaoqin Wang, Kyung Ho Row
Different kinds of deep eutectic solvents (DES) based on choline chloride (ChCl) and ionic liquids (ILs) based on 1-methylimidazole were used to modify Fe3O4/molecularly imprinted polymers (Fe3O4/MIPs), and the resulting materials were applied for the rapid purification of alkaloid isomers (theobromine and theophylline) from green tea with magnetic solid-phase extraction (M-SPE). The M-SPE procedure was optimized using the response surface methodology (RSM) to analyze the maximum conditions. The materials were characterized by Fourier transform infrared spectroscopy (FI-IR) and field emission scanning electron microscopy (FE-SEM)...
June 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28668496/generation-of-nanobodies-against-slyd-and-development-of-tools-to-eliminate-this-bacterial-contaminant-from-recombinant-proteins
#7
Yaozhong Hu, Ema Romão, Didier Vertommen, Cécile Vincke, Francisco Morales-Yánez, Carlos Gutiérrez, Changxiao Liu, Serge Muyldermans
The gene for a protein domain, derived from a tumor marker, fused to His tag codons and under control of a T7 promotor was expressed in E. coli strain BL21 (DE3). The recombinant protein was purified from cell lysates through immobilized metal affinity chromatography and size-exclusion chromatography. A contaminating bacterial protein was consistently co-purified, even using stringent washing solutions containing 50 or 100 mM imidazole. Immunization of a dromedary with this contaminated protein preparation, and the subsequent generation and panning of the immune Nanobody library yielded several Nanobodies of which 2/3 were directed against the bacterial contaminant, reflecting the immunodominance of this protein to steer the dromedary immune response...
June 28, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28665233/decellularization-of-whole-human-liver-grafts-using-controlled-perfusion-for-transplantable-organ-bioscaffolds
#8
Monique Verstegen, Jorke Willemse, Sjoerd van den Hoek, Gert-Jan Kremers, Theo M Luider, Nick A van Huizen, Francois E J A Willemssen, Herold J Metselaar, Jan Nm IJzermans, Luc J W van der Laan, Jeroen de Jonge
Liver transplantation is the only effective treatment for end-stage liver disease, but absolute donor shortage remains a limiting factor. Recent advances in tissue engineering focus on generation of native extracellular matrix (ECM) by decellularized complete livers in animal models. Though proof of concept has been reported for human livers, the current study aims to perform whole liver decellularization in a clinically relevant series using controlled machine perfusion. Here we describe a mild non-destructive decellularization protocol, effective in 11 discarded human whole liver grafts to generate constructs that reliably maintain hepatic architecture and ECM components using machine perfusion, while completely removing cellular DNA and RNA...
June 30, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28646090/cellular-functions-of-wasp-family-proteins-at-a-glance
#9
REVIEW
Olga Alekhina, Ezra Burstein, Daniel D Billadeau
Proteins of the Wiskott-Aldrich syndrome protein (WASP) family function as nucleation-promoting factors for the ubiquitously expressed Arp2/3 complex, which drives the generation of branched actin filaments. Arp2/3-generated actin regulates diverse cellular processes, including the formation of lamellipodia and filopodia, endocytosis and/or phagocytosis at the plasma membrane, and the generation of cargo-laden vesicles from organelles including the Golgi, endoplasmic reticulum (ER) and the endo-lysosomal network...
July 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28641660/-comparison-of-laboratory-indexes-and-therapeutic-efficacy-between-two-kinds-of-different-rbc-transfusion-in-aiha-patients
#10
Jing-Jing Gao, Xiong-Peng Zhu, Ming-Quan Wang
OBJECTIVE: To investigate the effect of 2 kinds of red blood cells (RBC) on the laboratorial indexes and therapeutic efficacy of patients with autoimmune hemolytic anemia (AIHA). METHODS: The clinical data of 120 patients with AIHA from June 2015 to June 2016 were analyzed retrospectively. These 120 patients were divided into A goup and B group. The patients in A group (60 cases) were infused with washed RBC, while the patients in B group (60 cases) were infused with WBC-deplated RBC...
June 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28636172/a-phos-tag-based-micropipette-tip-method-for-rapid-and-selective-enrichment-of-phosphopeptides
#11
Elena Tianfei Yuan, Yoko Ino, Maho Kawaguchi, Yayoi Kimura, Hisashi Hirano, Emiko Kinoshita-Kikuta, Eiji Kinoshita, Tohru Koike
Phosphorylated peptides are attractive targets in the study of the phosphoproteome. Here, we introduce a simple and convenient micropipette-tip method for the separation of phosphorylated and nonphosphorylated peptides by using a phosphate-binding zinc(II) complex of 1,3-bis(pyridin-2-ylmethylamino)propan-2-olate (Phos-tag). A 200-μL micropipette tip containing 10 μL of swollen agarose beads functionalized with Phos-tag moieties was prepared. All steps in the phosphate-affinity separation (binding, washing, and elution) were conducted by using aqueous buffers at neutral pH values...
June 21, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28631409/exploration-of-a-ternary-deep-eutectic-solvent-of-methyltriphenylphosphonium-bromide-chalcone-formic-acid-for-the-selective-recognition-of-rutin-and-quercetin-in-herba-artemisiae-scopariae
#12
Wanwan Ma, Tangand Baokun, Kyung Ho Row
A methyltriphenylphosphonium bromide/chalcone/formic acid as a green ternary deep eutectic solvent was applied as a functional monomer and dummy template simultaneously in the synthesis of a new molecularly imprinted polymer. Ternary deep eutectic solvent based molecularly imprinted polymers were used as a solid-phase extraction sorbent in the separation and purification of rutin and quercetin from Herba Artemisiae Scopariae combined with high-performance liquid chromatography. FTIR spectroscopy and field-emission scanning electron microscopy were applied to characterize the deep eutectic solvent based molecularly imprinted polymers synthesized using different molar ratios of chalcone...
June 20, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28626083/cellular-and-disease-functions-of-the-prader-willi-syndrome-gene-magel2
#13
REVIEW
Klementina Fon Tacer, Patrick Ryan Potts
Melanoma antigen L2 (MAGEL2 or MAGE-L2) is a member of the MAGE family of ubiquitin ligase regulators. It is maternally imprinted and often paternally deleted or mutated in the related neurodevelopmental syndromes, Prader-Willi Syndrome (PWS) and Schaaf-Yang Syndrome (SHFYNG). MAGEL2 is highly expressed in the hypothalamus and plays an important role in a fundamental cellular process that recycles membrane proteins from endosomes through the retromer sorting pathway. MAGEL2 is part of a multi-subunit protein complex consisting of MAGEL2, the TRIM27 E3 ubiquitin ligase, and the USP7 deubiquitinating enzyme...
June 16, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28624493/expression-of-the-c-terminal-domain-of-human-apolipoprotein-a-i-using-a-chimeric-apolipoprotein
#14
Daniel E Sallee, James V C Horn, Lukas A Fuentes, Paul M M Weers
Human apolipoprotein A-I (apoA-I) is the most abundant protein in high-density lipoprotein, an anti-atherogenic lipid-protein complex responsible for reverse cholesterol transport. The protein is composed of an N-terminal helix bundle domain, and a small C-terminal (CT) domain. To facilitate study of CT-apoA-I, a novel strategy was employed to produce this small domain in a bacterial expression system. A protein construct was designed of insect apolipophorin III (apoLp-III) and residues 179-243 of apoA-I, with a unique methionine residue positioned between the two proteins and an N-terminal His-tag to facilitate purification...
June 15, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28624152/simultaneous-determination-of-14-disinfection-by-products-in-meat-products-using-microwave-assisted-extraction-and-static-headspace-coupled-to-gas-chromatography-mass-spectrometry
#15
Maria Jose Cardador, Mercedes Gallego
This paper described the first analytical method to simultaneously determine 14 disinfection by-products (DBPs) in meat products using microwave-assisted extraction (MAE) and static headspace (SHS) followed by gas chromatography-mass spectrometry (GC-MS). The DBPs included were 4 trihalomethanes, 7 haloacetic acids, 2 haloacetonitriles and trichloronitromethane, which are commonly formed as a consequence of the disinfection process of water. The combination of the MAE and SHS techniques allows meat samples to be analysed in two sequential steps into the same HS vial in spite of the sample's complexity...
August 4, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28618384/a-fatty-acid-solvent-of-switchable-miscibility
#16
Qianqian Chen, Lei Wang, Gaihuan Ren, Qian Liu, Zhenghe Xu, Dejun Sun
Ion pair interactions were explored to design a fatty acid solvent of switchable miscibility with water. Fatty acids of medium length chains are immiscible with water but become miscible with water when ion pairs are formed with amines. The ion pairs become phase separated after bubbling CO2 into the solution due to the dissociation of the fatty acid-amine complexes. Ion pairs of caprylic acid (C8) and low toxic poly(oxypropylene) diamine (Jeffamine D-230) were characterized by FT-IR and (1)H NMR. Log Kow values and surface activity were used to understand the switchable solvent mechanism in removing and recovering oily contaminants...
June 7, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28618206/grazing-of-leaf-associated-cercomonads-protists-rhizaria-cercozoa-structures-bacterial-community-composition-and-function
#17
Sebastian Flues, David Bass, Michael Bonkowski
Preferential food selection in protists is well documented, but we still lack basic understanding on how protist predation modifies the taxonomic and functional composition of bacterial communities. We conducted feeding trials using leaf-associated cercomonad Cercozoa by incubating them on a standardized, diverse bacterial community washed from plant leaves. We used a shotgun metagenomics approach to investigate the taxonomic and functional changes of the bacterial community after five days protist predation upon bacteria...
June 15, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28605368/murine-short-axis-ventricular-heart-slices-for-electrophysiological-studies
#18
Gabriel Peinkofer, Juergen Hescheler, Marcel Halbach
Murine cardiomyocytes have been extensively used for in vitro studies of cardiac physiology and new therapeutic strategies. However, multicellular preparations of dissociated cardiomyocytes are not representative of the complex in vivo structure of cardiomyocytes, non-myocytes and extracellular matrix, which influences both mechanical and electrophysiological properties of the heart. Here we describe a technique to prepare viable ventricular slices of adult mouse hearts with a preserved in vivo like tissue structure, and demonstrate their suitability for electrophysiological recordings...
June 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28586917/toward-the-virtual-benchmarking-of-pneumatic-ventricular-assist-devices-application-of-a-novel-fluid-structure-interaction-based-strategy-to-the-penn-state-12-cc-device
#19
Alessandro Caimi, Francesco Sturla, Bryan Good, Marco Vidotto, Rachele De Ponti, Filippo Piatti, Keefe B Manning, Alberto Redaelli
The pediatric use of pneumatic ventricular assist devices (VADs) as a bridge to heart transplant still suffers for short-term major complications such as bleeding and thromboembolism. Although numerical techniques are increasingly exploited to support the process of device optimization, an effective virtual benchmark is still lacking. Focusing on the 12 cc Penn State pneumatic VAD, we developed a novel fluid-structure interaction (FSI) model able to capture the device functioning, reproducing the mechanical interplay between the diaphragm, the blood chamber, and the pneumatic actuation...
August 1, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28585814/carbazolic-porous-framework-with-tetrahedral-core-for-gas-uptake-and-tandem-detection-of-iodide-and-mercury
#20
Qin-Qin Dang, Hong-Jing Wan, Xian-Ming Zhang
A multifunctional carbazolic porous framework (Cz-TPM), with a tetrahedral core, has been synthesized by FeCl3 oxidative coupling polymerization. The Brunauer-Emmett-Teller surface area of the obtained polymers reaches 713.2 m(2) g(-1). Gas adsorption isotherms show that Cz-TPM exhibits large carbon dioxide (97.9 mg g(-1), 9.8 wt %, 273 K, and 1 bar) and hydrogen uptake capacities (149.3 cm(3) g(-1), 1.34 wt %, 77 K, and 1 bar). Furthermore, Cz-TPM has been found to display tandem visual detection of iodide and mercury, respectively...
June 15, 2017: ACS Applied Materials & Interfaces
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