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Wash complex

Rui-Cheng Gao, Fu-Sheng Guo, Nan-Nan Bai, Yun-Long Wu, Fan Yang, Ji-Ye Liang, Zhen-Jing Li, Yao-Yu Wang
Two new three-dimensional isostructural lanthanide metal-organic frameworks (Ln(III)-MOFs), [LnL(H2O)3]·3H2O·0.75DMF (1-Ln; Ln = Dy(III) and Eu(III) ions, H3L = biphenyl-3'-nitro-3,4',5-tricarboxylic acid, DMF = N,N'-dimethylformamide), were synthesized and characterized. The appearance of temperature-dependent out-of-phase (χ″M) signal reveals that complex 1-Dy displays slow magnetic relaxation behavior with the energy barrier (ΔUeff) of 57 K and a pre-exponential factor (τ0) of 3.89 × 10(-8) s at 1200 Oe direct current field...
October 19, 2016: Inorganic Chemistry
Qian Qi, Guoquan Yan, Chunhui Deng, Xiangmin Zhang
The process of protein digestion is a critical step for successful protein identification in proteomic analysis. Many efforts have been dedicated to enhancing the digestion efficiency for sufficient digestion. Among these approaches, protein complete denaturation with denaturants is a common process for better digestion. However, the removal of denaturants was tedious or would cause protein loss and other problems. In this work, a feasible digestion approach, immobilized protein digestion (IPD), based on covalent binding has been developed...
October 18, 2016: Analytical and Bioanalytical Chemistry
Jin Han Lim, Kyung Pyo Kang, Sik Lee, Sung Kwang Park, Won Kim
Heparin has remained the most commonly used anticoagulant for patients undergoing hemodialysis. It is usually safe to use but can have severe adverse effects in some cases. Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of exposure to heparin. It results from an autoantibody directed against endogenous platelet factor 4 (PF4) in complex with heparin, which activates platelets and can cause catastrophic arterial and venous thromboses. Here, we present the case of an 80-year-old woman with a recent diagnosis of chronic renal failure who developed acute HIT (platelet count nadir, 15 × 10(9) /L) on day 7 of hemodialysis performed with routine heparin anticoagulation, who despite subsequent heparin-free hemodialysis (with argatroban and warfarin) developed recurrent HIT (complicated by acute cerebral infarction) on day 11 that we attributed to "rinsing" of the circuit with heparin-containing saline (3,000 units of unfractionated heparin, with subsequent saline washing) performed pre-dialysis as per routine...
October 13, 2016: Hemodialysis International
Hongjun Chen, Zicheng Yu, Zhijuan Cao, Choiwan Lau
Magnetic bead (MB)-based chemiluminescence (CL) ELISA can be a sample-thrifty, time-saving tool for evaluation of cigarette smoke-induced DNA single-strand breaks (SSBs) with high specificity. This article describes a novel approach using immobilized oligonucleotide on MBs to determine cigarette smoke-induced DNA SSBs and screen some protective natural compounds. Typically, fluorescein-labeled DNA (FAM-DNA) was immobilized on the MBs and then oxidized by the smoke in the absence or presence of natural compounds, and a part of FAM-DNA was fragmented due to cigarette smoke-induced DNA SSB and then detached from MBs whereas other non-broken FAM-DNA still remained on MBs...
October 11, 2016: Analytical and Bioanalytical Chemistry
M A Jimoh, W MacNaughtan, H E L Williams, D Greetham, R L Linforth, I D Fisk
The nature of and factors effecting sodium interactions with psyllium were investigated in vitro. In a batch extraction system, psyllium mucilage gel retained at least 50% of sodium across a range of concentrations (5-300 mg sodium per g psyllium) and pH (2-10) environments. FTIR and Na NMR analyses of psyllium gels indicated that binding was complex with non-specific multi-site interactions. The potential use of psyllium husk as a binding agent for the reduction of bioavailable sodium was therefore evaluated...
September 14, 2016: Food & Function
Rizwan Ul Haq, Marlene Anderson, Agustin Liotta, Maria Shafiq, Muhammad Azhar Sherkheli, Uwe Heinemann
Norepinephrine, is involved in the enhancement of learning and memory formation by regulating synaptic mechanisms through its ability to activate pre- and post-synaptic adrenergic receptors. Here we show that β-agonists of norepinephrine facilitate the induction of both associational LTP and sharp wave ripples (SPW-Rs) in acute slices of rat hippocampus in area CA3. Surprisingly, this facilitating effect persists when slices are only pretreated with β-receptor agonists followed by wash out and application of the unspecific β-adrenoreceptor (βAR) antagonist propranolol...
October 3, 2016: Hippocampus
Yuan Wang, Yunqing Wang, Wenhai Wang, Kaoxiang Sun, Lingxin Chen
Reporter embedded (RE) tags are a new generation of sensitive, stable surface enhanced Raman scattering (SERS) tags with Raman reporters embedded between gold nanoparticle (NP) cores and gold (or silver) shells. Most of the reported RE tags have been designed using Au nanospheres as a seed material. Herein, we investigated the synthesis and SERS properties of AuNR/reporter/Ag tags by using gold nanorod (AuNR) seeds with anisotropic physical and optical features. Several highlighted points were discovered, including the following: 1) The cetyltrimethylammonium bromide (CTAB) layer induced the co-existence of chemically and physically adsorbed Raman reporters on AuNR...
October 3, 2016: ACS Applied Materials & Interfaces
Suku-Maran Shalini, Deron R Herr, Wei-Yi Ong
Pain and anxiety have a complex relationship and pain is known to share neurobiological pathways and neurotransmitters with anxiety. Top-down modulatory pathways of pain have been shown to originate from cortical and subcortical regions, including the dorsolateral prefrontal cortex. In this study, a novel docosahexaenoic acid (DHA)-containing nutraceutical, Souvenaid, was administered to mice with infraorbital nerve ligation-induced neuropathic pain and behavioral responses recorded. Infraorbital nerve ligation resulted in increased face wash strokes of the face upon von Frey hair stimulation, indicating increased nociception...
October 1, 2016: Molecular Neurobiology
Imogen E Napper, Richard C Thompson
Washing clothes made from synthetic materials has been identified as a potentially important source of microscopic fibres to the environment. This study examined the release of fibres from polyester, polyester-cotton blend and acrylic fabrics. These fabrics were laundered under various conditions of temperature, detergent and conditioner. Fibres from waste effluent were examined and the mass, abundance and fibre size compared between treatments. Average fibre size ranged between 11.9 and 17.7μm in diameter, and 5...
November 15, 2016: Marine Pollution Bulletin
Shengjun Wu
Pullulan possesses uniquely good structural flexibility and solubility. Surimi is an aquatic product prepared from minced fish flesh, which is washed to remove most lipids, blood, enzymes and sarcoplasmic proteins. However, data on effect of pullulan on gel properties of fish surimi are limited. This study investigated the influence of pullulan at different concentrations (0-2g/100 g wet materials) on the properties of Scomberomorus niphonius surimi gel including the water holding capacity, adhesiveness, cohesiveness and microstructure...
September 23, 2016: International Journal of Biological Macromolecules
Keith W Vance
Capture Hybridization Analysis of RNA Targets (CHART) has recently been developed to map the genome-wide binding profile of chromatin-associated RNAs. This protocol uses a small number of 22-28 nucleotide biotinylated antisense oligonucleotides, complementary to regions of the target RNA that are accessible for hybridization, to purify RNAs from a cross-linked chromatin extract. RNA-chromatin complexes are next immobilized on beads, washed, and specifically eluted using RNase H. Associated genomic DNA is then sequenced using high-throughput sequencing technologies and mapped to the genome to identify RNA-chromatin associations on a large scale...
2017: Methods in Molecular Biology
S Grether-Beck, A Marini, T Jaenicke, W Stahl, J Krutmann
BACKGROUND: Increasing evidence suggests photo-protection by oral supplementation with ß-carotene and lycopene. OBJECTIVES: We examined the capacity of lycopene rich tomato nutrient complex (TNC) and lutein, to protect against UVA/B- and UVA1 radiation at a molecular level. METHODS: In a placebo-controlled, double blinded, randomized cross over study two actives containing either TNC or lutein were assessed for their capacity to decrease the expression of UVA1 radiation-inducible genes including heme oxygenase-1 (HO-1), intercellular adhesion molecule-1 (ICAM-1) and matrix metalloproteinase-1 (MMP-1)...
September 23, 2016: British Journal of Dermatology
Catherine M Buckley, Navin Gopaldass, Cristina Bosmani, Simon A Johnston, Thierry Soldati, Robert H Insall, Jason S King
Macropinocytosis is an ancient mechanism that allows cells to harvest nutrients from extracellular media, which also allows immune cells to sample antigens from their surroundings. During macropinosome formation, bulk plasma membrane is internalized with all its integral proteins. It is vital for cells to salvage these proteins before degradation, but the mechanisms for sorting them are not known. Here we describe the evolutionarily conserved recruitment of the WASH (WASP and SCAR homolog) complex to both macropinosomes and phagosomes within a minute of internalization...
October 4, 2016: Proceedings of the National Academy of Sciences of the United States of America
Lung-Chieh Chen, Cheng-Chiang Chen, Kai-Chieh Liang, Sheng Hsiung Chang, Zhong-Liang Tseng, Shih-Chieh Yeh, Chin-Ti Chen, Wen-Ti Wu, Chun-Guey Wu
Nano-structured CuO-Cu2O complex thin film-based perovskite solar cells were fabricated on an indium tin oxide (ITO)-coated glass and studied. Copper (Cu) thin films with a purity of 99.995 % were deposited on an ITO-coated glass by magnetron reactive sputtering. To optimize the properties of the nano-structured CuO-Cu2O complex thin films, the deposited Cu thin films were thermally oxidized at various temperatures from 300 to 400 °C. A CH3NH3PbI3 perovskite absorber was fabricated on top of CuO-Cu2O complex thin film by a one-step spin-coating process with a toluene washing treatment...
December 2016: Nanoscale Research Letters
Aoli Wang, Xiao-E Yan, Hong Wu, Wenchao Wang, Chen Hu, Cheng Chen, Zheng Zhao, Peng Zhao, Xixiang Li, Li Wang, Beilei Wang, Zi Ye, Jinhua Wang, Chu Wang, Wei Zhang, Nathanael S Gray, Ellen L Weisberg, Liang Chen, Jing Liu, Cai-Hong Yun, Qingsong Liu
Ibrutinib, a clinically approved irreversible BTK kinase inhibitor for Mantle Cell Lymphoma (MCL) and Chronic Lymphocytic Leukemia (CLL) etc, has been reported to be potent against EGFR mutant kinase and currently being evaluated in clinic for Non Small Cell Lung Cancer (NSCLC). Through EGFR wt/mutant engineered isogenic BaF3 cell lines we confirmed the irreversible binding mode of Ibrutinib with EGFR wt/mutant kinase via Cys797. However, comparing to typical irreversible EGFR inhibitor, such as WZ4002, the washing-out experiments revealed a much less efficient covalent binding for Ibrutinib...
September 10, 2016: Oncotarget
Peter M Benz, Hebatullah Laban, Joana Zink, Lea Günther, Ulrich Walter, Stepan Gambaryan, Karim Dib
BACKGROUND: Vasodilator-Stimulated Phosphoprotein (VASP) is involved in the inhibition of agonist-induced platelet aggregation by cyclic nucleotides and the adhesion of platelets to the vascular wall. αIIbβ3 is the main integrin responsible for platelet activation and Rap1b plays a key role in integrin signalling. We investigated whether VASP is involved in the regulation of Rap1b in platelets since VASP-null platelets exhibit augmented adhesion to endothelial cells in vivo. METHODS: Washed platelets from wild type and VASP-deficient mice were stimulated with thrombin, the purinergic receptors agonist ADP, or the thromboxane A2 receptor agonist U46619 and Rap1b activation was measured using the GST-RalGDS-RBD binding assay...
2016: Cell Communication and Signaling: CCS
Sajjad Husain Mir, Bungo Ochiai
A facile one-pot approach for preparing hierarchical nanowire-networks of hollow polymer@Pd nanospheres is reported. First, polymer@Pd hollow nanospheres were produced through metal-complexation-induced phase separation with functionalized graft copolymers and subsequent self-assembly of PdNPs. The nanospheres hierarchically assembled into the nanowire-network upon drying. The Pd nanowire-network served as an active catalyst for Mizoroki-Heck and Suzuki-Miyaura coupling reactions. As low as 500 μmol % Pd was sufficient for quantitative reactions, and the origin of the high activity is ascribed to the highly active sites originating from high-index facets, kinks, and coalesced structures...
June 2016: ChemistryOpen
Carlo Tremolada, Valeria Colombo, Carlo Ventura
In the past few years, interest in adipose tissue as an ideal source of mesenchymal stem cells (MSCs) has increased. These cells are multipotent and may differentiate in vitro into several cellular lineages, such as adipocytes, chondrocytes, osteoblasts, and myoblasts. In addition, they secrete many bioactive molecules and thus are considered "mini-drugstores." MSCs are being used increasingly for many clinical applications, such as orthopedic, plastic, and reconstructive surgery. Adipose-derived MSCs are routinely obtained enzymatically from fat lipoaspirate as SVF and/or may undergo prolonged ex vivo expansion, with significant senescence and a decrease in multipotency, leading to unsatisfactory clinical results...
2016: Current Stem Cell Reports
Walter Schön, Florian Mittermayr, Albrecht Leis, Irene Mischak, Martin Dietzel
The chemical and isotopic composition of soil solutions is highly relevant for environmental and forensic tasks. We investigated interstitial solutions from soil horizons of three cambisols in Styria (Austria). The soils consisted mainly of quartz, feldspar and clay minerals with a vertical variability. Two soil solution fractions from meso-, macro- and micropores (m) and micropores only (μ) were extracted at two subsequent hydraulic pressure steps corresponding to matrix potentials of up to pF 5.43 and from 5...
August 16, 2016: Science of the Total Environment
Ashraf Fakhari, Amir R Jalilian, Hassan Yousefnia, Saeed Shanehsazzadeh, Ali Bahrami Samani, Fariba Johari Daha, Mehdi Shafiee Ardestani, Ali Khalaj
OBJECTIVE: Optimized production and quality control of ytterbium-175 (Yb-175) labeled pamidronate and alendronate complexes as efficient agents for bone pain palliation has been presented. METHODS: Yb-175 labeled pamidronate and alendronate (175Yb-PMD and 175Yb-ALN) complexes were prepared successfully at optimized conditions with acceptable radiochemical purity, stability and significant hydroxyapatite absorption. The biodistribution of complexes were evaluated up to 48 h, which demonstrated significant bone uptake ratios for 175Yb-PAM at all-time intervals...
October 5, 2015: Molecular Imaging and Radionuclide Therapy
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