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https://www.readbyqxmd.com/read/28549267/the-construction-of-a-novel-nucleic-acids-detection-microplatform-based-on-the-nset-for-one-step-detecting-tk1-dna-and-microrna-21
#1
Jian Zhang, Qian Zhao, Yudong Wu, Bo Zhang, Weipan Peng, Jiafang Piao, Yurui Zhou, Weichen Gao, Xiaoqun Gong, Jin Chang
Microbeads-based microchip technology has become the potential for a new generation of nucleic acids detection in a high-throughput and sensitive manner. However the specificity and operational complexity limit the microchip applied in nucleic acids detection. Herein, in this work, we designed a kind of gold-nanoparticles coated polystyrene microbeads as microplatform conjugating with the molecular beacons as probes. Due to the nanoparticle surface energy transfer of gold-nanoparticles, the fluorescence of dye on one end of molecular beacons was effectively quenched...
May 23, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28541034/microfluidic-capillaric-circuit-for-rapid-and-facile-bacteria-detection
#2
Ayokunle Oluwafemi Olanrewaju, Andy Ng, Philippe Decorwin-Martin, Alessandra Robillard, David Juncker
Urinary tract infections (UTI) are one of the most common bacterial infections and would greatly benefit from a rapid point-of-care diagnostic test. Although significant progress has been made in developing microfluidic systems for nucleic acid and whole bacteria immunoassay tests, their practical application is limited by complex protocols, bulky peripherals, and slow operation. Here we present a microfluidic capillaric circuit (CC) optimized for rapid and automated detection of bacteria in urine. Molds for CCs were constructed using previously-established design rules, then 3D-printed and replicated into poly(dimethylsiloxane)...
May 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28528500/using-poly-glutamic-acid-as-soil-washing-agent-to-remediate-heavy-metal-contaminated-soils
#3
Zong-Han Yang, Cheng-Di Dong, Chiu-Wen Chen, Yih-Terng Sheu, Chih-Ming Kao
The extraction efficiency of heavy metals from soils using three forms of gamma poly-glutamic acid (γ-PGA) as the washing agents was investigated. Controlling factors including agent concentrations, extraction time, pH, and liquid to soil ratio were evaluated to determine the optimum operational conditions. The distribution of heavy metal species in soils before and after extraction processes was analyzed. Up to 46 and 74% of heavy metal removal efficiencies were achieved with one round and a sequential extraction process using H-bonding form of γ-PGA (200 mM) with washing time of 40 min, liquid to solid ratio of 10 to 1, and pH of 6...
May 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28521061/extracellular-camp-activates-molecular-signalling-pathways-associated-with-sperm-capacitation-in-bovines
#4
Carlos Agustín I Alonso, Claudia E Osycka-Salut, Luciana Castellano, Andreína Cesari, Nicolás Di Siervi, Adrián Mutto, Anders Johannisson, Jane M Morrell, Carlos Davio, Silvina Perez-Martinez
STUDY QUESTION: Is extracellular cAMP involved in the regulation of signalling pathways in bovine sperm capacitation? SUMMARY ANSWER: Extracellular cAMP-induced sperm capacitation through the activation of different signalling pathways that involve phospholipase C (PLC), PKC/ERK1-2 signalling and an increase in sperm Ca2+ levels, as well as soluble AC and cAMP/protein kinase A (PKA) signalling. WHAT IS KNOWN ALREADY: In order to fertilize the oocyte, ejaculated spermatozoa must undergo a series of changes in the female reproductive tract, known as capacitation...
May 18, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28510331/isolation-of-ready-to-use-adipose-derived-stem-cell-asc-pellet-for-clinical-applications-and-a-comparative-overview-of-alternate-methods-for-asc-isolation
#5
Edoardo Raposio, Nicolò Bertozzi
Current literature does not offer a standardized method to isolate adipose-derived stem cells (ASCs) for clinical applications and hence clinical studies using ASCs often show inconsistent results. Most of these studies borrow laboratory or benchside-derived protocols, which are complex, time consuming, and involve the use of chemical, animal-derived reagents. In this unit we describe a relatively simple and faster isolation protocol that allows collection of a ready-to-use ASC pellet for clinical application...
May 16, 2017: Current Protocols in Stem Cell Biology
https://www.readbyqxmd.com/read/28503993/dual-inhibition-of-pi3k-and-mtor-by-vs-5584-suppresses-thrombus-formation
#6
Thomas Später, Isabelle Müller, Hermann Eichler, Michael D Menger, Matthias W Laschke, Emmanuel Ampofo
VS-5584 is a highly selective dual kinase inhibitor which suppresses phosphatidylinositol 3-kinase (PI3K) and mammalian target of rapamycin (mTOR) activity. Because these kinases are crucially involved in primary hemostasis, we herein investigated the effect of this compound on thrombus formation in vitro and in vivo. Pretreatment of washed platelets (WP) or platelet-rich plasma (PRP) with VS-5584 inhibited the agonist-induced activation of surface glycoprotein complex (GP)IIb/IIIa and the upregulation of P-selectin...
May 15, 2017: Platelets
https://www.readbyqxmd.com/read/28500949/human-health-risks-for-legionella-and-mycobacterium-avium-complex-mac-from-potable-and-non-potable-uses-of-roof-harvested-rainwater
#7
Kerry A Hamilton, Warish Ahmed, Simon Toze, Charles N Haas
A quantitative microbial risk assessment (QMRA) of opportunistic pathogens Legionella pneumophila (LP) and Mycobacterium avium complex (MAC) was undertaken for various uses of roof-harvested rainwater (RHRW) reported in Queensland, Australia to identify appropriate usages and guide risk management practices. Risks from inhalation of aerosols due to showering, swimming in pools topped up with RHRW, use of a garden hose, car washing, and toilet flushing with RHRW were considered for LP while both ingestion (drinking, produce consumption, and accidental ingestion from various activities) and inhalation risks were considered for MAC...
April 5, 2017: Water Research
https://www.readbyqxmd.com/read/28489394/surface-engineering-of-ultrathin-gold-nanowires-tailored-self-assembly-and-enhanced-stability
#8
El Said Abd ElFattah Nouh, Edwin A Baquero, Lise-Marie Lacroix, Fabien Delpech, Romuald Poteau, Guillaume Viau
Gold nanowires with a mean diameter of 1.7 nm were synthesized by reduction of HAuCl4 in a solution of oleylamine (OY) in hexane. A bilayer of oleylammonium chloride/oleylamine at the surface of the raw nanowires was evidenced by NMR and DOSY experiments. After washing a monolayer of oleylammonium chloride remained at the surface of the nanowires. The oleylammonium chloride layer could be progressively replaced by a phosphine shell as evidenced NMR and Diffusion Ordered Spectroscopy experiment (DOSY) experiments, in good agreement with the adsorption energies given by DFT calculations...
May 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28458618/a-review-on-platelet-activating-factor-inhibitors-could-a-new-class-of-potent-metal-based-anti-inflammatory-drugs-induce-anticancer-properties
#9
REVIEW
Vasiliki D Papakonstantinou, Nefeli Lagopati, Effie C Tsilibary, Constantinos A Demopoulos, Athanassios I Philippopoulos
In this minireview, we refer to recent results as far as the Platelet Activating Factor (PAF) inhibitors are concerned. At first, results of organic compounds (natural and synthetic ones and specific and nonspecific) as inhibitors of PAF are reported. Emphasis is given on recent results about a new class of the so-called metal-based inhibitors of PAF. A small library of 30 metal complexes has been thus created; their anti-inflammatory activity has been further evaluated owing to their inhibitory effect against PAF in washed rabbit platelets (WRPs)...
2017: Bioinorganic Chemistry and Applications
https://www.readbyqxmd.com/read/28456637/a-fast-method-for-high-resolution-oxymetry-study-of-skeletal-muscle-mitochondrial-respiratory-chain-complexes
#10
Jean-Claude Vienne, Catherine Cimetta, Marie Dubois, Thibault Duburcq, Raphaël Favory, Anne-Frédérique Dessein, Monique Fontaine, Marie Joncquel-Chevalier Curt, Jean-Marie Cuisset, Claire Douillard, Karine Mention-Mulliez, Dries Dobbelaere, Joseph Vamecq
High resolution oxymetry study (HROS) of skeletal muscle usually requires 90-120 min preparative phase (dissection, permeabilization and washing). This work reports on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minute using an ultra-dispersor apparatus. Subsequent HROS is performed using FCCP instead of ADP, compounds crossing and not plasma membrane, respectively...
April 26, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28453321/hand-decontamination-in-clinical-practice-a-review-of-the-evidence
#11
Ruth Rigby, Anne Pegram, Sue Woodward
Hand decontamination is known to significantly reduce the spread of hospital-acquired infections but, despite a wealth of guidelines and education campaigns, evidence suggests that many healthcare workers are non-compliant with hand decontamination. The behaviours that prevent hand decontamination are complex. Studies look at attitudes towards dirt, disgust, self-protective hand washing and familiarity with patients. Self-protection behaviours manifest themselves in washing hands more often after certain tasks...
April 27, 2017: British Journal of Nursing: BJN
https://www.readbyqxmd.com/read/28449947/barcoding-of-gpcr-trafficking-and-signaling-through-the-various-trafficking-roadmaps-by-compartmentalized-signaling-networks
#12
REVIEW
Suleiman W Bahouth, Mohammed M Nooh
Proper signaling by G protein coupled receptors (GPCR) is dependent on the specific repertoire of transducing, enzymatic and regulatory kinases and phosphatases that shape its signaling output. Activation and signaling of the GPCR through its cognate G protein is impacted by G protein-coupled receptor kinase (GRK)-imprinted "barcodes" that recruit β-arrestins to regulate subsequent desensitization, biased signaling and endocytosis of the GPCR. The outcome of agonist-internalized GPCR in endosomes is also regulated by sequence motifs or "barcodes" within the GPCR that mediate its recycling to the plasma membrane or retention and eventual degradation as well as its subsequent signaling in endosomes...
April 24, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28448438/antiplatelet-activity-of-a-newly-synthesized-novel-ruthenium-ii-a-potential-role-for-akt-jnk-signaling
#13
Themmila Khamrang, Kuo-Chen Hung, Chih-Hsuan Hsia, Cheng-Ying Hsieh, Marappan Velusamy, Thanasekaran Jayakumar, Joen-Rong Sheu
In oncotherapy, ruthenium complexes are considered as potential alternatives for platinum compounds, and have been proved as promising anticancer drugs with high efficacy and lesser side effects. Platelet activation plays a major role in cancer metastasis and progression. Hence, this study explored the effect of a newly synthesized ruthenium complex, [Ru(η⁶-cymene)(L)Cl]BF₄(TQ5), where L = 4-phenyl-2-pyridin-2-yl-quinazoline), on human platelet activation. TQ5 (3-5 µM) inhibited concentration-dependent collagen-induced platelet aggregation in washed human platelets...
April 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28441599/transport-and-attenuation-of-salmonella-enterica-fecal-indicator-bacteria-and-a-poultry-litter-marker-gene-are-correlated-in-soil-columns
#14
Sirisha Mantha, Angela Anderson, Saraswati Poudel Acharya, Valerie J Harwood, Jennifer Weidhaas
Millions of tons of fecal-contaminated poultry litter are applied to U.S. agricultural fields annually. Precipitation and irrigation facilitate transport of fecal-derived pathogens and fecal indicator bacteria (FIB) to groundwater. The goal of this study was to compare transport of pathogens, FIB, and a microbial source tracking marker gene for poultry litter (LA35) in a simulated soil-to-groundwater system. Nine laboratory soil columns containing four different soil types were used to evaluate microbial transport to groundwater via infiltration...
April 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28429416/modulation-of-heterotypic-and-homotypic-cell-cell-interactions-via-zwitterionic-lipid-masks
#15
Matthew Park, Wongu Youn, Doyeon Kim, Eun Hyea Ko, Beom Jin Kim, Sung Min Kang, Kyungtae Kang, Insung S Choi
Since the pioneering work by Whitesides, innumerable platforms that aim to spatio-selectively seed cells and control the degree of cell-cell interactions in vitro have been developed. These methods, however, have generally been technically and methodologically complex, or demanded stringent materials and conditions. In this work, we introduce zwitterionic lipids as patternable, cell-repellant masks for selectively seeding cells. The lipid masks are easily removed with a routine washing step under physiological conditions (37 °C, pH 7...
April 21, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28425967/a-simple-and-specific-noncompetitive-elisa-method-for-ht-2-toxin-detection
#16
Henri O Arola, Antti Tullila, Alexis V Nathanail, Tarja K Nevanen
We developed an HT-2 toxin-specific simple ELISA format with a positive read-out. The assay is based on an anti-immune complex (IC) scFv antibody fragment, which is genetically fused with alkaline phosphatase (AP). The anti-IC antibody specifically recognizes the IC between a primary anti-HT-2 toxin Fab fragment and an HT-2 toxin molecule. In the IC ELISA format, the sample is added together with the scFv-AP antibody to the ELISA plate coated with the primary antibody. After 15 min of incubation and a washing step, the ELISA response is read...
April 20, 2017: Toxins
https://www.readbyqxmd.com/read/28424231/mroh1-a-lysosomal-regulator-localised-by-wash-generated-actin
#17
Peter A Thomason, Jason S King, Robert H Insall
The steps leading to constitutive exocytosis are poorly understood. In Dictyostelium WASH complex mutants, exocytosis is blocked, so cells that take up fluorescent dextran from the medium retain it and remain fluorescent. Here we establish a FACS-based method to select cells that retain fluorescent dextran, allowing identification of mutants with disrupted exocytosis. Screening a pool of random mutants identified the WASH complex, as expected, and multiple mutants in the conserved HEAT-repeat containing protein Mroh1...
April 19, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28411649/nanoarmoring-of-enzymes-by-interlocking-in-cellulose-fibers-with-poly-acrylic-acid
#18
Caterina M Riccardi, Rajeswari M Kasi, Challa V Kumar
A simple method for interlocking glucose oxidase (GOx) and horseradish peroxidase (HRP) in cellulose fibers using poly(acrylic acid) (PAA) as an armor around the enzyme, without any need for activation of the cellulose support, is reported here. The resulting enzyme paper is an inexpensive, stable, simple, wearable, and washable biosensor. PAA functions as a multifunctional tether to interlock the enzyme molecules around the paper fibers so that the enzymes are protected against thermal/chemical denaturation and not released from the paper when washed with a detergent...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28402305/the-binding-of-metal-ions-to-molecularly-imprinted-polymers
#19
Rukshan Perera, Syed Ashraf, Anja Mueller
Imprinting polymerization is a flexible method to make resins specific for different compounds. Imprinting polymerization involves the polymerization of the resin in the presence of a template, here cadmium ions or arsenate. The template is then removed by washing, leaving specific binding sites in the resin. In water treatment, the removal of toxic metal ions is difficult due to the limited affinity of these ions to ion exchange resins. Imprinting polymerization of ion-exchange resins is used to develop resins with high capacity and some selectivity for cadmium ions or arsenate for water treatment that still function as general ion-exchange resins...
April 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28399507/the-emerging-role-of-retromer-in-neuroprotection
#20
REVIEW
Kirsty J McMillan, Hendrick C Korswagen, Peter J Cullen
Efficient sorting and transportation of integral membrane proteins, such as ion channels, nutrient transporters, signalling receptors, cell-cell and cell-matrix adhesion molecules is essential for the function of cellular organelles and hence organism development and physiology. Retromer is a master controller of integral membrane protein sorting and transport through one of the major sorting station within eukaryotic cells, the endosomal network. Subtle de-regulation of retromer is an emerging theme in the pathoetiology of Parkinson's disease...
April 8, 2017: Current Opinion in Cell Biology
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