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https://www.readbyqxmd.com/read/28723962/surface-functionalization-specific-binding-of-coagulation-factors-by-zinc-oxide-nanoparticles-delays-coagulation-time-and-reduces-thrombin-generation-potential-in-vitro
#1
Jun-Young Yang, Jiyeong Bae, Ayoung Jung, Seonyeong Park, Seungtae Chung, Jihyun Seok, Hangsik Roh, Youngju Han, Jae-Min Oh, Soojung Sohn, Jayoung Jeong, Wan-Seob Cho
Zinc oxide nanoparticles (ZnO NPs) have many biomedical applications such as chemotherapy agents, vaccine adjuvants, and biosensors but its hemocompatibility is still poorly understood, especially in the event of direct contact of NPs with blood components. Here, we investigated the impact of size and surface functional groups on the platelet homeostasis. ZnO NPs were synthesized in two different sizes (20 and 100 nm) and with three different functional surface groups (pristine, citrate, and L-serine). ZnO NPs were incubated with plasma collected from healthy rats to evaluate the coagulation time, kinetics of thrombin generation, and profile of levels of coagulation factors in the supernatant and coronated onto the ZnO NPs...
2017: PloS One
https://www.readbyqxmd.com/read/28723173/laser-scribed-graphene-electrodes-for-aptamer-based-biosensing
#2
Christoph Fenzl, Pranati Nayak, Thomas Hirsch, Otto S Wolfbeis, Husam N Alshareef, Antje J Baeumner
Graphene as a transducer material has produced some of the best-performing sensing approaches to date opening the door toward integrated miniaturized all-carbon point-of-care devices. Addressing this opportunity, laser-scribed graphene (LSG) electrodes are demonstrated here as highly sensitive and reliable biosensor transducers in blood serum analysis. These flexible electrodes with large electrochemical surface areas were fabricated using a direct-write laser process on polyimide foils. A universal immobilization approach is established by anchoring 1-pyrenebutyric acid to the graphene and subsequently covalently attaching an aptamer against the coagulation factor thrombin as an exemplary bioreceptor to the carboxyl groups...
May 26, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28723117/versatile-barometer-biosensor-based-on-au-pt-core-shell-nanoparticle-probe
#3
Qiangqiang Fu, Ze Wu, Dan Du, Chengzhou Zhu, Yuehe Lin, Yong Tang
There is a high global demand for sensitive, portable, user-friendly, and cost-effective biosensors. In this work, we introduce a barometer-based biosensor for the detection of a broad range of targets. The device is operated by measuring the pressure change produced by oxygen (O2) generation in a limited chamber using a portable barometer. The design employs core-shell Au@Pt nanoparticles (Au@PtNPs) as the bioassay probe to catalyze the decomposition of H2O2 and the release of O2. As a proof of concept, we developed barometer-based immunosensors to detect carcinoembryonic antigen (CEA) and ractopamine (Rac)...
June 23, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28722442/sensing-active-heparin-by-counting-aggregated-quantum-dots-at-single-particle-level
#4
Suli Dong, Xiaojun Liu, Qingquan Zhang, Wenfeng Zhao, Chenghua Zong, Aiye Liang, Hongwei Gai
Developing highly sensitive and highly selective assays for monitoring heparin levels in blood is required during and after surgery. In previous studies, electrostatic interactions are exploited to recognize heparin and changes in light signal intensity are used to sense heparin. In the present study, we developed a quantum dot (QD) aggregation-based detection strategy to quantify heparin. When cationic micelles and fluorescence QDs modified with anti-thrombin III (AT III) are added into heparin sample solution, the AT III-QDs, which specifically bind with heparin, aggregate around the micelles...
January 27, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28720509/the-neuropeptide-galanin-promotes-an-anti-thrombotic-phenotype-on-endocardial-endothelial-cells-from-heart-failure-patients
#5
Christina Tyrrell, Amanda Toyooka, Faiza Khan, Kent L Thornburg, James O Mudd, Wohaib Hasan
Thromboembolic complications are a significant cause of mortality and re-hospitalization in heart failure (HF) patients. One source of thrombi is the ventricular endocardial surface that becomes increasingly pro-thrombotic as HF progresses. Anticoagulation comes with bleeding risks so identifying therapeutic agents for improving cardiac endothelial health are of critical clinical importance. Endocardial endothelial cells are closely apposed to cardiac sympathetic nerves. In HF, cardiac sympathetic nerves are dysregulated and promote disease progression...
July 12, 2017: Autonomic Neuroscience: Basic & Clinical
https://www.readbyqxmd.com/read/28719611/rna-sequencing-to-determine-the-contribution-of-kinase-receptor-transactivation-to-g-protein-coupled-receptor-signalling-in-vascular-smooth-muscle-cells
#6
Danielle Kamato, Venkata Vijayanand Bhaskarala, Nitin Mantri, Tae Gyu Oh, Dora Ling, Reearna Janke, Wenhua Zheng, Peter J Little, Narin Osman
G protein coupled receptor (GPCR) signalling covers three major mechanisms. GPCR agonist engagement allows for the G proteins to bind to the receptor leading to a classical downstream signalling cascade. The second mechanism is via the utilization of the β-arrestin signalling molecule and thirdly via transactivation dependent signalling. GPCRs can transactivate protein tyrosine kinase receptors (PTKR) to activate respective downstream signalling intermediates. In the past decade GPCR transactivation dependent signalling was expanded to show transactivation of serine/threonine kinase receptors (S/TKR)...
2017: PloS One
https://www.readbyqxmd.com/read/28718695/enhanced-procoagulant-activity-of-platelets-after-chemotherapy-in-non-small-cell-lung-cancer
#7
Ruishuang Ma, Yayan Bi, Junjie Kou, Jin Zhou, Jialan Shi
The procoagulant status of patients with non-small cell lung cancer (NSCLC) after chemotherapy is poorly characterized and the role of platelets in hypercoagulative state of NSCLC is unknown. The aim of this study was to evaluate the procoagulant activity (PCA) of platelets in NSCLC before and after chemotherapy. The subjects were 52 patients newly diagnosed with NSCLC. The patients had decreased clotting time compared with healthy subjects, and the thrombin-antithrombin complex increased 2.5 fold after chemotherapy...
July 18, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/28718456/graphene-oxide-and-dna-aptamer-based-sub-nanomolar-potassium-detecting-optical-nanosensor
#8
Debopam Datta, Ketaki Sarkar, Souvik Mukherjee, Xenia Meshik, Michael A Stroscio, Mitra Dutta
Quantum-dot (QD) based nanosensors are frequently used by researchers to detect small molecules, ions and different biomolecules. In this article, we present a sensor complex/system comprised of deoxyribonucleic acid (DNA) aptamer, gold nanoparticle and semiconductor QD, attached to a graphene oxide (GO) flake for detection of potassium. As reported herein, it is demonstrated that QD-aptamer-quencher nanosensor functions even when tethered to GO, opening the way to future applications where sensing can be accomplished simultaneously with other previously demonstrated applications of GO such as serving as a nanocarrier for drug delivery...
August 11, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28713823/intracardiac-hemostasis-and-fibrinolysis-parameters-in-patients-with-atrial-fibrillation
#9
Noémi Klára Tóth, Zoltán Csanádi, Orsolya Hajas, Alexandra Kiss, Edina Nagy-Baló, Kitti Bernadett Kovács, Ferenc Sarkady, László Muszbek, Zsuzsanna Bereczky, László Csiba, Zsuzsa Bagoly
AIMS: To identify intracardiac hemostasis or fibrinolysis abnormalities, which are associated with atrial fibrillation (AF) and increase the risk of thromboembolism. PATIENTS AND METHODS: Patient group consisted of 24 patients with AF and control group included 14 individuals with other supraventricular tachycardia undergoing transcatheter radiofrequency ablation. Blood samples were drawn from the femoral vein (FV), left atrium (LA), and left atrial appendage (LAA) before the ablation procedure...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28713563/recent-advances-in-the-treatment-of-venous-thromboembolism-in-the-era-of-the-direct-oral-anticoagulants
#10
REVIEW
Jeffrey I Weitz, Iqbal H Jaffer, James C Fredenburgh
The direct oral anticoagulants (DOACs) have now supplanted vitamin K antagonists (VKAs) for the treatment of venous thromboembolism (VTE). The DOACs include dabigatran, which inhibits thrombin, and rivaroxaban, apixaban, and edoxaban, which inhibit factor Xa. The DOACs are as effective for the prevention of recurrence as conventional VTE treatment, consisting of a parenteral anticoagulant followed by a VKA, and are associated with less bleeding. Because of these properties and the convenience of fixed dosing without the need for routine coagulation monitoring, guidelines now recommend DOACs over VKAs for VTE treatment in patients without active cancer...
2017: F1000Research
https://www.readbyqxmd.com/read/28712479/antiplatelet-aggregation-activity-of-walnut-hull-extract-via-suppression-of-reactive-oxygen-species-generation-and-caspase-activation
#11
Azadeh Meshkini, Masoumeh Tahmasbi
Walnut hull (wal hull) is an agricultural by-product that is widely used in traditional medicine for alleviating pain and treating skin diseases, however, recently it has gained much attention in modern pharmacology due to its antioxidant properties. The current study was aimed to determine the total phenolic, flavonoid, and tannin content of Persian wal hull extract and evaluate its biological effects on platelet function. Experimental data showed that acetone extract of wal hulls has a high content of polyphenolic compounds and antioxidant properties...
June 2017: Journal of Acupuncture and Meridian Studies
https://www.readbyqxmd.com/read/28711993/clinical-risk-assessment-in-the-antiphospholipid-syndrome-current-landscape-and-emerging-biomarkers
#12
REVIEW
Shruti Chaturvedi, Keith R McCrae
PURPOSE OF REVIEW: Laboratory criteria for the classification of antiphospholipid syndrome include the detection of a lupus anticoagulant and/or anticardiolipin and anti-β2-glycoprotein I antibodies. However, the majority of patients who test positive in these assays do not have thrombosis. Current risk-stratification tools are largely limited to the antiphospholipid antibody profile and traditional thrombotic risk factors. RECENT FINDINGS: Novel biomarkers that correlate with disease activity and potentially provide insight into future clinical events include domain 1 specific anti-β2GPI antibodies, antibodies to other phospholipids or phospholipid/protein antigens (such as anti-PS/PT), and functional/biological assays such as thrombin generation, complement activation, levels of circulating microparticles, and annexin A5 resistance...
July 2017: Current Rheumatology Reports
https://www.readbyqxmd.com/read/28711012/osteopontin-in-hepatocellular-carcinoma-a-possible-biomarker-for-diagnosis-and-follow-up
#13
Manuela Cabiati, Melania Gaggini, Maria Michela Cesare, Chiara Caselli, Paolo De Simone, Franco Filipponi, Giuseppina Basta, Amalia Gastaldelli, Silvia Del Ry
Recently osteopontin (OPN), a protein of the extracellular matrix, has generated in hepatocellular carcinoma (HCC) a significant interest as a prognostic factor. Aim of this study was to confirm, in liver tissues of subjects with HCV-positive HCC undergoing liver transplantation (RL, n=10) and of donors (DL, n=14), the increase of OPN plasma and tissue concentration, the OPN splicing isoforms expression profiling together with those of thrombin, and to evaluate a possible association between OPN measurements...
July 12, 2017: Cytokine
https://www.readbyqxmd.com/read/28710679/new-phage-display-isolated-heptapeptide-recognizing-the-regulatory-carboxy-terminal-domain-of-human-tumour-protein-p53
#14
Sihem Ben Abid, Mouna Sahnoun, Ines Yacoubi-Hadj Amor, Salma Abdelmoula-Souissi, Hajer Hassairi, Raja Mokdad-Gargouri, Ali Gargouri
The transcription factor tumor protein p53 (P53) controls a variety of genes most involved in cell cycle and is at the origin of apoptosis when DNA is irreparably damaged. We planned to select novel tumor protein p53-interacting peptides through the screening of hepta-peptide phage-display libraries. For this aim, human tumor suppressor protein p53 was expressed in Escherichia coli as Glutathione S-transferase fusion and purified by affinity chromatography. The phage library was then screened on this immobilized protein target...
July 14, 2017: Protein Journal
https://www.readbyqxmd.com/read/28707386/efficacy-and-safety-of-a-coagulated-thrombus-injection-for-peripheral-artery-perforation-the-coagulated-thrombus-hemostasis-method
#15
Takahiro Tokuda, Keisuke Hirano, Masahiro Yamawaki, Motoharu Araki, Norihiro Kobayashi, Shinsuke Mori, Yasunari Sakamoto, Hideyuki Takimura, Masakazu Tsutsumi, Yoshiaki Ito
BACKGROUND: This retrospective study assesses the safety and efficacy of using a thrombus injection for treatment a peripheral artery perforation. METHODS AND RESULTS: From March 2013 to April 2016, we performed 1,152 endovascular treatment (EVT) at our hospital. Of these, 28 peripheral artery perforations occurred during the EVT procedure and 20 were treated with a thrombus, which we artificially created. The thrombus comprised the patient's blood and thrombin...
July 14, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28705901/temporal-contribution-of-the-platelet-body-and-balloon-to-thrombin-generation
#16
Ejaife O Agbani, Ingeborg Hers, Alastair W Poole
No abstract text is available yet for this article.
July 13, 2017: Haematologica
https://www.readbyqxmd.com/read/28705337/-treatment-of-post-catheterization-pseudoaneurysms-by-ultrasound-guided-thrombin-injection-a-single-center-experience-and-practical-guideline
#17
G Böge, J P Laroche, P Alric
Ultrasound-guided thrombin injection has been shown to be a safe and effective treatment for iatrogenic post-catheterization pseudoaneurysms, but still is underused in France. We report our single-center experience and propose a technical guideline for ultrasound-guided thrombin injection. Ultrasound-guided thrombin injection should be considered to be the first-line treatment of iatrogenic pseudoaneurysms.
July 2017: J Med Vasc
https://www.readbyqxmd.com/read/28704925/licochalcone-a-prevents-platelet-activation-and-thrombus-formation-through-the-inhibition-of-plc%C3%AE-2-pkc-akt-and-mapk-pathways
#18
Li-Ming Lien, Kuan-Hung Lin, Li-Ting Huang, Mei-Fang Tseng, Hou-Chang Chiu, Ray-Jade Chen, Wan-Jung Lu
Platelet activation is involved in cardiovascular diseases, such as atherosclerosis and ischemic stroke. Licochalcone A (LA), an active ingredient of licorice, exhibits multiple biological activities such as anti-oxidation and anti-inflammation. However, its role in platelet activation remains unclear. Therefore, the study investigated the antiplatelet mechanism of LA. Our data revealed that LA (2-10 μM) concentration dependently inhibited platelet aggregation induced by collagen, but not thrombin and U46619...
July 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28704111/incomplete-dabigatran-reversal-with-idarucizumab
#19
Aaron P Steele, Jin A Lee, William E Dager
CONTEXT: With increasing use of direct oral anticoagulants (DOACs), urgent reversal of these agents becomes a growing concern. Idarucizumab is a humanized monoclonal antibody fragment that specifically binds to dabigatran with higher affinity than thrombin, rapidly neutralizing its anticoagulant effect without increased risk of thrombosis. CASE DETAILS: We describe two cases in which the recommended dose of idarucizumab was unsuccessful in completely reversing the anticoagulant effects of dabigatran...
July 13, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28703014/thrombin-receptor-agonist-peptide-induced-platelet-aggregation-is-reduced-in-patients-receiving-dabigatran
#20
Frantisek Nehaj, Juraj Sokol, Michal Mokan, Jela Ivankova, Maros Mokan
The availability of direct oral anticoagulants has caused a paradigm shift in thrombosis management. The direct thrombin inhibitor dabigatran seems to obstruct tenase complex by inhibiting thrombin generated in the initial phase and feed back to the amplification phase of cell-based coagulation reactions. However, it is still not fully understood if and how dabigatran impact platelet function. This observational study aimed to assess in vitro platelet function in patients with atrial fibrillation receiving dabigatran...
January 1, 2017: Clinical and Applied Thrombosis/hemostasis
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