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Heterotopic transplantation ovary

Deepa Bhartiya, Sandhya Anand, Seema Parte
BACKGROUND: Infertility is an undesirable side effect and gonadal tissue banking is advocated in young cancer patients who are unable to preserve embryos or gametes prior to oncotherapy to achieve biological parenthood later on. Banking gonadal tissue is challenging and protocols to mature gametes in vitro are not yet clinically established. Transplanting ovarian cortical tissue at hetero-or orthotopic sites in women and bone marrow transplantation (BMT) in both men and women has resulted in spontaneous recovery of fertility, pregnancy and live births...
2015: Journal of Ovarian Research
Won-Jun Choi, Jin-Sook Seok, In-Young Choi, Ji-Kwon Park, Jeong-Kyu Shin, Soon-Ae Lee, Won-Young Paik, Jong-Hak Lee
OBJECTIVE: Revascularization is critical for successful ovarian tissue transplantation. Vascular endothelial growth factor (VEGF) and angiopoietin-2 (angpt-2) are the principal mediators of neovascularization. This study was designed to assess VEGF and angpt-2 levels in cryopreserved ovarian tissue after heterotopic autotransplantation. METHODS: Ovarian tissues harvested from ICR mice at 5 to 6 weeks of age were stratified as follows: no cryopreservation (controls, group I); vitrification in VFS-40 (vitrification, group II); and gradual freezing in dimethyl sulfoxide (slow-freezing, group III)...
September 2015: Obstetrics & Gynecology Science
Monireh Mahmoodi, Malek Soleimani Mehranjani, Seyed Mohammad Ali Shariatzadeh, Hussein Eimani, Abdulhussein Shahverdi
The effect of N-acetylcysteine (NAC) on mouse ovary heterotopic autotransplantation was investigated. Mice (age 4-5 weeks) were divided into the following groups: control; autograft plus NAC (150 mg/kg daily intraperitoneal injection) and autograft plus saline (n = 6 per group). Groups were treated from 1 day before until 7 days after transplantation. After 28 days, ovary compartments were estimated stereologically. Plasma malondialdehyde, progesterone, oestradiol concentrations and the percentage of apoptotic follicles were measured to evaluate the rate of oxidative stress and ovarian graft function...
January 2015: Reproductive Biomedicine Online
Jacques Donnez, Marie-Madeleine Dolmans
Since the first live birth after orthotopic transplantation of frozen-thawed ovarian tissue, >40 babies have been born. It is time to consider fertility preservation in women as one of the foremost challenges of the next decade and to offer women facing the risk of induced or iatrogenic premature menopause the best chances of becoming mothers. Heterotopic transplantation has also been attempted, with consistent restoration of endocrine function; nonetheless, its clinical value remains questionable as it may not provide an optimal environment for follicular development, possibly because of differences in temperature, pressure, paracrine factors and blood supply...
November 2014: Best Practice & Research. Clinical Obstetrics & Gynaecology
N S Abtahi, H Eimani, A Vosough, Ab Shahverdi, R Fathi, N Hayati, N Nasiri
One of the challenges in ovarian transplantation is ischemia-reperfusion damage. When transitional tissue faces an acute and critical condition in terms of blood supply (immediately after organ transplantation), treatment with low-intensity pulsed ultrasound (LIPUS) seems to be very beneficial. The aim of this study was to evaluate the effects of ultrasound therapy on heterotopic transplanted mouse ovarian tissue. Adult female Naval Medical Research Institute mice were divided into three groups. In the experimental groups, the transplanted ovary was exposed 5 min daily to ultrasound with an intensity of 0...
July 2014: Ultrasound in Medicine & Biology
Arash Behbahanian, Hossein Eimani, Bahman Zeinali, Mojtaba Rezazadeh Valojerdi, Poopak Eftekhari Yazdi, Abdolhossein Shahverdi, Hamid Gourabi, Afsaneh Golkar-Narenji
BACKGROUND: The purpose of this study was to investigate the in vitro survival and developmental potential of oocytes obtained from vitrified mouse ovaries transplanted to a heterotopic site. MATERIALS AND METHODS: In this experimental study, two-week-old mice were unilaterally ovariectomized after anesthesia. The ovaries were vitrified by cryotop. After two weeks, the ovaries were thawed and autotransplanted to the gluteus muscle tissue. Three weeks later the mice were killed, after which we removed and dissected the transplanted and opposite right ovaries...
January 2013: International Journal of Fertility & Sterility
S Samuel Kim
Scepticism hovers over the future of heterotopic transplantation of cryopreserved human ovarian tissue, as its clinical efficacy and practicability for fertility preservation is still debatable. Despite its limitations, the potential advantages and roles of heterotopic transplantation should not be ignored. Indeed, restoration of ovarian function after heterotopic transplantation of cryopreserved ovarian tissue has been consistently demonstrated in humans. There are many unknowns with this technology, such as the optimal heterotopic site, environmental factors and oocyte quality...
February 2014: Reproductive Biomedicine Online
C J Stern, D Gook, L G Hale, F Agresta, J Oldham, G Rozen, T Jobling
Ovarian tissue cryopreservation and transplantation is a form of fertility preservation offered to young women at high risk of losing ovarian function after cancer treatment. While there have been successful births resulting from orthotopic site grafts, we report the first case of an ongoing pregnancy from a heterotopic graft in a patient who had previously undergone bilateral oopherectomy for a granulosa cell tumour. Frozen-thawed ovarian tissue was transplanted to the anterior abdominal wall. Subsequent ovarian stimulation and transabdominal ultrasound-guided oocyte retrieval from the grafts resulted in two oocytes...
November 2013: Human Reproduction
Jiang-Man Gao, Jie Yan, Rong Li, Min Li, Li-Ying Yan, Tian-Ren Wang, Hong-Cui Zhao, Yue Zhao, Yang Yu, Jie Qiao
STUDY QUESTION: Does basic fibroblast growth factor (bFGF) in combination with fibrin hydrogel improve follicle development and revascularization of heterotopically transplanted mouse ovarian tissues? SUMMARY ANSWER: Treatment of transplanted ovarian tissues with higher concentrations (75, 100 and 150 μg/ml), but not lower concentrations (25 and 50 μg/ml), of bFGF significantly improved primordial follicle survival and angiogenesis, while apoptosis of follicles and stromal cells was significantly decreased...
October 2013: Human Reproduction
Iu O Tishchenko, V V Kiroshka
We examined the dynamics of folliculogenesis in ovarian tissue allograft and its endocrine function in an organism of castrated recipient rats during 100 days. Growth and development of ovarian tissue and its steroidogenic function in heterotopic transplantation were determined by its initial morphological organization. Allotransplantation of mature ovarian tissue was found to cause preservation of its morphological structure, whereas the transplantation of neonatal ovarian tissue caused formation of follicular cysts...
2013: Fiziolohichnyĭ Zhurnal
A Y Ting, R R Yeoman, J R Campos, M S Lawson, S F Mullen, G M Fahy, M B Zelinski
STUDY QUESTION: What are the appropriate conditions to vitrify the macaque ovarian cortex in a large-volume, closed system that will preserve functional pre-antral follicles? SUMMARY ANSWER: The combination of glycerol, ethylene glycol (EG) and polymers with cooling in liquid nitrogen (LN2) vapor and a two-step warming procedure was able to preserve tissue and follicle morphology as well as function of a small population of secondary follicles in the macaque ovarian cortex following vitrification in a closed system...
May 2013: Human Reproduction
A Gougeon
The purpose of this review is to present the strategies for fertility preservation that are currently proposed to patients at risk from premature ovarian failure (POF) due to chemotherapy as well as some future solutions that could allow patients already affected by an IOP to recover their fertility. Today, cryo-preservation of pieces of ovarian cortex followed by orthotopic transplantation as well as in vitro maturation of oocytes (IVM) allow preservation of fertility in patients being to undergo an antitumor treatment...
November 2012: Gynécologie, Obstétrique & Fertilité
Hong-Yan Wang, Yun-Hong Li, Lei Sun, Xuan Gao, Li You, Yin Wang, Jing-Long Ma, Zi-Jiang Chen
OBJECTIVE: To evaluate the genetic safety of vitrification on the methylation imprints and the development and fertility potential of prepubertal mouse ovaries. DESIGN: Experimental animal study. SETTING: University-based fertility center. ANIMAL(S): Institute of Cancer Research (ICR) 10-day-old female mice, 10-week-old adult female mice, and 12-week-old adult male mice. INTERVENTION(S): Vitrification of juvenile mouse ovaries was performed using ED20 and EG5...
January 2013: Fertility and Sterility
Julie Vanacker, Valérie Luyckx, Marie-Madeleine Dolmans, Anne Des Rieux, Jonathan Jaeger, Anne Van Langendonckt, Jacques Donnez, Christiani A Amorim
For women diagnosed with leukemia, transplantation of cryopreserved ovarian tissue after disease remission is not advisable. Therefore, to restore fertility in these patients, we aim to develop a biodegradable artificial ovary that offers an environment where isolated follicles and ovarian cells (OCs) can survive and grow. Four NMRI mice were ovariectomized and their ovaries used to isolate OCs. Groups of 50,000 OCs were embedded in an alginate-matrigel matrix for further fixation (fresh controls), one week of in vitro culture (IVC) or heterotopic autografting...
September 2012: Biomaterials
Nao Suzuki, Shu Hashimoto, Suguru Igarashi, Seido Takae, Masaya Yamanaka, Takayuki Yamochi, Makoto Takenoshita, Yoshihiko Hosoi, Yoshiharu Morimoto, Bunpei Ishizuka
BACKGROUND: Ovarian tissue cryopreservation by rapid cooling (vitrification) is a convenient fertility preservation option. However, the progress of vitrified ovarian tissue after transplantation is not well understood in primates. METHODS: For tissues from cynomolgus monkeys, we used closed straw vitrification and open cryosupport vitrification in which tissues are immersed directly into liquid nitrogen. Following warming, ovarian cortical pieces were autotransplanted and their function was monitored by computed tomography (CT), hormone assays and oocyte recovery, ICSI and embryo transfers (ETs)...
August 2012: Human Reproduction
V V Kiroshka, Iu O Tishchenko
In this work, a comparative analysis of dynamics of morphological development and endocrine function of transplants of sexually mature and neonatal ovarian tissue was performed under conditions of bilateral ovariectomy of animals-recipients. It has been established that at transplantation of the sexually mature ovarian tissue there were observed all stages of folliculogenesis and preservation of endocrine function at long-term observation (up to 100 days). At transplantation of neonatal ovarian tissue there was revealed a pathological picture of its development: hyperplasia of stromal structure and formation of cysts and cystomae...
March 2012: Zhurnal Evoliutsionnoĭ Biokhimii i Fiziologii
Deepa Bhartiya, Kalpana Sriraman, Seema Parte
The spontaneous return of fertility after bone marrow transplantation or heterotopic grafting of cryopreserved ovarian cortical tissue has surprised many, and a possible link with stem cells has been proposed. We have reviewed the available literature on ovarian stem cells in adult mammalian ovaries and presented a model that proposes that the ovary harbors two distinct populations of stem cells, namely, pluripotent, quiescent, very small embryonic-like stem cells (VSELs), and slightly larger "progenitor" ovarian germ stem cells (OGSCs)...
2012: Obstetrics and Gynecology International
Yanrong Wang, Qing Chang, Jing Sun, Ling Dang, Wenzhi Ma, Changchun Hei, Xinsheng Shen, Chengjun Zhao, Yufang Cai, Xiuying Pei, Xiaoguang Zhang, Yin Wang, Xiaohua Jiang
Ovarian tissue transplantation is now considered as a procedure to preserve the fertility of young women patients undergoing cancer therapy. The present study investigated the effects and mechanism of human menopausal gonadotrophin (HMG) intervention on vascular remoulding in ovarian heterotopic autotransplantation. Ovaries of 8-week-old mice were cultured in vitro with different concentrations of HMG for 3h for measuring the expression of vascular endothelial growth factor (VEGF). The cultured ovaries were implanted under the kidney capsule and removed 24, 36, 48 h or 1 month after transplantation...
June 2012: Reproductive Biomedicine Online
S Samuel Kim
PURPOSE: To assess the longevity of ovarian grafts in five cancer patients who underwent heterotopic autotransplantation of frozen-thawed ovarian tissue. METHOD(S): Five cancer survivors underwent heterotopic ovarian transplantation between 2001 and 2011. Stored ovarian tissue (for 1-10 years) was rapidly thawed and transplanted into the space between the rectus sheath and the rectus muscle (8-20 cortical sections per patient). Endocrine function was assessed by monthly blood tests (FSH, LH, E2, progesterone and testosterone) and ultrasound after transplantation...
June 2012: Journal of Assisted Reproduction and Genetics
Munira Xaymardan, Zhuo Sun, Kota Hatta, Masaki Tsukashita, Filip Konecny, Richard D Weisel, Ren-Ke Li
We evaluated the hypothesis that uterine cells home to the heart after injury and improve cardiac outcomes. Premenopausal women have fewer cardiovascular complications than age-matched men, but the mechanisms responsible for this protection have not been conclusively identified. Hysterectomy was performed in young female rats (leaving the ovaries intact), and 7 days later the left coronary artery was ligated to produce a myocardial infarction (MI). Cardiac function at 28 days post-MI was measured using echocardiography...
June 2012: Journal of Molecular and Cellular Cardiology
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