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https://www.readbyqxmd.com/read/28711355/characterization-of-the-foxl2-proximal-promoter-and-coding-sequence-from-the-common-snapping-turtle-chelydra-serpentina
#1
Lei Guo, Turk Rhen
Sex is determined by temperature during embryogenesis in snapping turtles, Chelydra serpentina. Previous studies in this species show that dihydrotestosterone (DHT) induces ovarian development at temperatures that normally produce males or mixed sex ratios. The feminizing effect of DHT is associated with increased expression of FoxL2, suggesting that androgens regulate transcription of FoxL2. To test this hypothesis, we cloned the proximal promoter (1.6kb) and coding sequence for snapping turtle FoxL2 (tFoxL2) in frame with mCherry to produce a fluorescent reporter...
July 12, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28574993/weigh-the-pros-and-cons-to-ovarian-reserve-before-stripping-ovarian-endometriomas-prior-to-ivf-icsi-a-meta-analysis
#2
Xin Tao, Lei Chen, Shuqi Ge, Lisi Cai
PURPOSE: To explore the effects of conservative surgery for endometriomas on ovarian responsiveness during assisted reproductive technology (ART) and provide reproductive and gynecological doctors with a more reliable reference program for the treatment of endometriomas. METHODS: A literature search was performed by searching the PubMed, Embase, Cochrane Library, Web of Science and Science Direct databases. Studies with inter- and intra-patient comparisons of ovarian responses and oocyte quality between operated and unoperated ovaries and that met the inclusion criteria were retrieved, and the data from the outcome measures were extracted and pooled for this meta-analysis...
2017: PloS One
https://www.readbyqxmd.com/read/28486360/ovarian-mucinous-cystadenoma-after-ovarian-graft
#3
Carole Fajau-Prevot, Yann Tanguy Le Gac, Christine Chevreau, Clémentine Cohade, Nicolas Gatimel, Jean Parinaud, Roger Leandri
BACKGROUND: Freezing strips of ovarian cortex before chemotherapy followed by transplantation is an experimental method to preserve fertility for reproductive-aged women with cancer. We report a case of a cancer patient who developed a mucinous cystadenoma in a grafted piece of ovarian cortex. CASE: A 32-year-old woman with a Ewing sarcoma had ovarian cryopreservation using cortical strip freezing before receiving chemotherapy. Five years later she had no ovarian function, and the strips were thawed and grafted back onto the ovary...
June 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28421599/surgery-versus-conservative-management-of-endometriomas-in-subfertile-women-a-systematic-review
#4
Jacob Brink Laursen, Jeppe Bennekou Schroll, Kirsten T Macklon, Martin Rudnicki
INTRODUCTION: Endometriomas are present in up to 44% of all women with endometriosis and have a detrimental effect on fertility. However, it is controversial whether endometriomas should be surgically removed before assisted reproduction technology (ART). Our purpose was to evaluate whether surgical stripping of endometriomas in subfertile women improves the chance of a live birth. Secondary outcomes were impact on ovarian reserve and pain. Material and methods We conducted a systematic review and metaanalysis with results reported in accordance to the PRISMA guidelines...
April 19, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28272468/accuracy-and-safety-verification-of-ovarian-reserve-assessment-technique-for-ovarian-tissue-transplantation-using-optical-coherence-tomography-in-mice-ovary
#5
Seido Takae, Kosuke Tsukada, Yorino Sato, Naoki Okamoto, Tai Kawahara, Nao Suzuki
Except for histological study, there are currently no suitable techniques available for the detection and identification of primordial follicles in ovary of primary ovarian insufficiency patients who have undetectable AMH levels. Also, the ability to locate and quantify follicles on ovarian cortex strips, without fixation, is valuable for patients who could undergo subsequent successful ovarian tissue transplantation. Although optical coherence tomography (OCT) is a well-established high resolution imaging technique without fixation commonly applied in biomedicine, few reports are available on ovarian tissue imaging...
March 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/27932268/ten-principles-for-safe-surgical-treatment-of-ovarian-endometriosis
#6
Manuel Antonio López de la Torre, Henrique M Abrao, Luiz F Fernandes, Rosanne M Kho, Mauricio S Abrao
STUDY OBJECTIVE: To show a step-by-step laparoscopic approach for excision of an ovarian endometrioma following surgical principles for safety and maximal preservation of ovarian function. DESIGN: Video. Medical management of ovarian endometriomas is not recommended. Operative laparoscopy is the treatment of choice. Although considered a simple procedure, ovarian cystectomy requires a precise and correct technique in order to preserve ovarian function. SETTING: A private hospital...
February 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/27888061/secretory-function-of-ovarian-cells-and-myometrial-contractions-in-cow-are-affected-by-chlorinated-insecticides-chlordane-heptachlor-mirex-in-vitro
#7
Michael Hubert Wrobel, Jaroslaw Mlynarczuk
The aim of the study was to investigate the effect of chlordane, heptachlor and mirex, on hormonal regulation of the force of myometrial contractions. Myometrial, endometrial, granulosa and luteal cells as well as strips of myometrium from non-pregnant cows were incubated with three insecticides at environmentally relevant doses (0.1, 1 or 10ng/ml). None of the insecticides affected the viability of studied cells. Chlordane stimulated, while heptachlor and mirex inhibited, secretion of testosterone and estradiol from granulosa cells as well as secretion of progesterone from luteal cells, respectively...
January 1, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/27884789/surgery-for-advanced-epithelial-ovarian-cancer
#8
REVIEW
Neville F Hacker, Archana Rao
Cytoreductive surgery for patients with advanced epithelial ovarian cancer has been practised since the pioneering work of Tom Griffiths in 1975. Further research has demonstrated the prognostic significance of the extent of metastatic disease pre-operatively, and of complete cytoreduction post-operatively. Patients with advanced epithelial ovarian cancer should be referred to high volume cancer units, and managed by multidisciplinary teams. The role of thoracoscopy and resection of intrathoracic disease is presently investigational...
May 2017: Best Practice & Research. Clinical Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/27866379/effects-of-oral-exposure-to-the-phthalate-substitute-acetyl-tributyl-citrate-on-female-reproduction-in-mice
#9
Lindsay M Rasmussen, Nivedita Sen, Xiaosong Liu, Zelieann R Craig
Acetyl tributyl citrate (ATBC), is a phthalate substitute used in food and medical plastics, cosmetics and toys. Although systemically safe up to 1000 mg kg(-1)  day(-1) , its ability to cause reproductive toxicity in females at levels below 50 mg kg(-1)  day(-1) has not been examined. This study evaluated the effects of lower ATBC exposures on female reproduction using mice. Adult CD-1 females (n = 7-8 per treatment) were dosed orally with tocopherol-stripped corn oil (vehicle), 5 or 10 mg kg(-1)  day(-1) ATBC daily for 15 days, and then bred with a proven breeder male...
June 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/27836207/primary-debulking-surgery-of-the-upper-abdomen-and-the-diaphragm-with-a-plasma-device-surgery-system-for-advanced-ovarian-cancer
#10
Gloria Cordeiro Vidal, Guillaume Babin, Denis Querleu, Frédéric Guyon
OBJECTIVE: Electrically neutral argon plasma (PlasmaJet™) technology is a surgical option that helps to get an aggressive cytoreduction in selected patients with ovarian cancer because it can be directly applied by the surgeon to treat the tissue surface [1,2]. Upper abdominal surgical procedures are an important part of the surgery in these patients [3], there is a 22% complications rate when they are performed [4]. We present a surgical approach to ovarian cancer debulking using PlasmaJet™...
January 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/27649578/aspiration-of-immature-oocytes-during-cesarean-section-for-fertility-preservation
#11
Avi Ben-Haroush, Ronit Abir, Onit Sapir, Roni Garor, Benjamin Fisch
BACKGROUND: In vitro maturation (IVM) of immature oocytes is an important technology for selected clinical indications. We previously described a pregnant woman with a history of renal transplantation who underwent oocyte aspiration during cesarean section (CS) for fertility preservation and future surrogacy. CASE: A 27-year-old pregnant woman was diagnosed with neck rhabdomyosarcoma at 37 weeks' gestation. CS was performed with direct aspiration of small follicles from one ovary and oophorectomy of the other...
March 28, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27630317/diaphragmatic-surgery-in-advanced-ovarian-tubal-and-peritoneal-cancer-a-7-year-retrospective-analysis-of-the-tumor-bank-ovarian-cancer-network
#12
Mustafa Zelal Muallem, Jumana Almuheimid, Rolf Richter, Elena Ioana Braicu, Suzan Osman, Jalid Sehouli
AIM: This study aimed to analyze diaphragmatic interventions and their complications in primary cytoreductive surgery (PCS) and to study their impact on attaining complete tumor resection (CTR) in advanced ovarian cancer (AOC), which is purportedly reflected in better disease-free and overall survival. PATIENTS AND METHODS: The study's collective consisted of 536 consecutive patients presenting a first diagnosis of AOC who underwent PCS between 2007 and 2013 at the Charité Medical University, Berlin...
2016: Anticancer Research
https://www.readbyqxmd.com/read/27542707/recurrent-massive-ascites-due-to-mossy-endometriosis
#13
REVIEW
Erica C Dun, Serena Wong, Nisha A Lakhi, Ceana H Nehzat
OBJECTIVE: To report the medical and surgical management of a rare case of recurrent moss-like endometriosis and associated hemorrhagic ascites. DESIGN: Video description of the case, demonstration of the surgical technique, discussion of the histology, and review of endometriosis-associated ascites. SETTING: Tertiary referral center. PATIENT(S): A 26-year-old nulliparous woman of Nigerian heritage with recurrent hemorrhagic ascites due to endometriosis...
November 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27488214/current-south-african-clinical-practice-in-debulking-surgery-for-ovarian-cancer
#14
Jana Billson, F Haynes van der Merwe, Robbert P Soeters
INTRODUCTION: The aim of this study was to assess practice patterns and rationale with regard to debulking surgery for advanced epithelial ovarian carcinoma among South African Gynaecological oncologists. METHODS: A survey was distributed to all practicing gynecological oncologists in South Africa. It was structured to assess definitions of optimal debulking, procedures used to achieve optimal debulking, and reasons for not performing specific procedures. Respondents were also asked to report on experience and additional surgical training to improve skill in performing ultra-radical debulking surgery...
October 2016: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/27436368/which-should-be-the-preferred-technique-during-laparoscopic-ovarian-cystectomy-hemostatic-sutures-or-bipolar-electrocoagulation-a-randomized-controlled-prospective-study-of-long-term-ovarian-reserve
#15
Cagdas Sahin, Ali Akdemir, Ahmet Mete Ergenoglu, Banu Ozgurel, Ahmet Ozgur Yeniel, Dilek Taskiran, Fatih Sendag
The aim of the present study was to determine the long-term effects of different laparoscopic hemostatic techniques on ovarian reserve after ovarian cystectomy. Ninety patients with unilateral ovarian cysts were recruited and randomly distributed into 2 groups. Laparoscopic stripping cystectomy was performed in all patients. Afterward, cystectomy hemostasis was achieved via hemostatic suture or bipolar electrocoagulation. Serum levels of anti-Müllerian hormone (AMH) were determined preoperatively and postoperatively at 1, 3, and 12 months, and patients were evaluated for residual ovarian volume, antral follicle count, and pregnancy...
July 19, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27428495/pelvic-congestion-syndrome-and-left-renal-compression-syndrome-clinical-features-and-therapeutic-approaches
#16
REVIEW
Christina Jeanneret, Konstantin Beier, Alexander von Weymarn, Jürg Traber
Knowledge of the anatomy of the pelvic, gonadal and renal veins is important to understand pelvic congestion syndrome (PCS) and left renal vein compression syndrome (LRCS), which is also known as the nutcracker syndrome. LRCS is related to PCS and to the presence of vulvar, vaginal and pudendal varicose veins. The diagnosis of the two syndromes is difficult, and usually achieved with CT- or phlebography. The gold standard is the intravenous pressure measurement using conventional phlebography. The definition of PCS is described as pelvic pain, aggravated in the standing position and lasting for more than 6 months...
2016: VASA. Zeitschrift Für Gefässkrankheiten
https://www.readbyqxmd.com/read/27054965/fertility-outcome-after-laparoscopic-treatment-of-advanced-endometriosis-in-two-groups-of-infertile-patients-with-and-without-ovarian-endometrioma
#17
COMPARATIVE STUDY
Adel Shervin, Arash Mohazzab, Mina Aminlou, Koorosh Kamali, Roya Padmehr, Khadijeh Shadjoo, Parvin Jaberi-Pour, Elham Akbari
OBJECTIVE: To evaluate the result of laparoscopic endometrioma excision in fertility outcome of advanced endometriosis patients. STUDY DESIGN: The study was designated as historical cohort, in a private referral center of advance laparoscopy. 111 infertile patients, diagnosed as endometriosis, were divided in two groups: DIE (deep infiltrative endometriosis) and endometrioma (case group), and patients with only DIE (without endometrioma ((control group). All patients underwent global laparoscopic resection of DIE lesion (both groups) and laparoscopic excisional cystectomy of endometrioma (case groups)...
June 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27040418/maximal-effort-cytoreductive-surgery-for-disseminated-ovarian-cancer-in-a-uk-setting-challenges-and-possibilities
#18
Christina Fotopoulou, Benjamin P Jones, Konstantinos Savvatis, Jeremy Campbell, Maria Kyrgiou, Alan Farthing, Stephen Brett, Rene Roux, Marcia Hall, Gordon Rustin, Hani Gabra, Long Jiao, Richard Stümpfle
OBJECTIVE: To assess surgical morbidity and mortality of maximal effort cytoreductive surgery for disseminated epithelial ovarian cancer (EOC) in a UK tertiary center. METHODS/MATERIALS: A monocentric prospective analysis of surgical morbidity and mortality was performed for all consecutive EOC patients who underwent extensive cytoreductive surgery between 01/2013 and 12/2014. Surgical complexity was assessed by the Mayo clinic surgical complexity score (SCS). Only patients with high SCS ≥5 were included in the analysis...
September 2016: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/26979065/heterotopic-ovarian-transplantation-results-in-less-apoptosis-than-orthotopic-transplantation-in-a-minipig-model
#19
COMPARATIVE STUDY
Lia Cruz V C Damásio, José Maria Soares-Júnior, Jairo Iavelberg, Gustavo A R Maciel, Manuel de Jesus Simões, Ricardo Dos Santos Simões, Eduardo Vieira da Motta, Maria Cândida Pinheiro Baracat, Edmund C Baracat
BACKGROUND: Ovarian autotransplantation has shown increasing promise as a clinical method for the preservation of fertility and hormonal function. However, information regarding the success rate of this type of transplantation is limited. We hypothesized that results vary according to the site of the ovarian transplantation. To test this hypothesis, fresh or cryopreserved ovarian strips were autotransplanted to orthotopic or heterotopic sites. The strips were later collected, and the morphology and expression of selected markers of apoptosis were evaluated...
March 15, 2016: Journal of Ovarian Research
https://www.readbyqxmd.com/read/26969445/the-effect-of-fsh-and-activin-a-on-akt-and-mapk1-3-phosphorylation-in-cultured-bovine-ovarian-cortical-strips
#20
Filiz Tepekoy, Gokhan Akkoyunlu
BACKGROUND: rhFSH and rhActA have been used in mammalian ovarian follicle culture systems for activation of follicular growth in vitro and suggested to be responsible for primordial follicle survival through MAPK and Akt pathways. The aim of our study was to determine the effects of rhFSH and rhActA on Akt, pAkt, MAPK1/3 and pMAPK1/3 protein levels in bovine ovarian cortical strips cultured in vitro. METHODS: Ovarian cortical strips from heifers were cultured in the presence of rhFSH (50 ng/mL), rhActA (100 ng/mL) or combination of these factors for 6 days...
March 11, 2016: Journal of Ovarian Research
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