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Ovarian strips

Avi Ben-Haroush, Ronit Abir, Onit Sapir, Roni Garor, Benjamin Fisch
BACKGROUND: In vitro maturation of immature oocytes is an important technology for selected clinical indications. We previously described a pregnant woman with a history of renal transplantation who underwent oocyte aspiration during cesarean section (CS) for fertility preservation and future surrogacy. CASE: A 27-year-old pregnant woman was diagnosed with neck rhabdomyosarcoma at 37 weeks' gestation. CS was performed with direct aspiration of small follicles from one ovary and oophorectomy of the other...
September 21, 2016: Journal of Maternal-fetal & Neonatal Medicine
Mustafa Zelal Muallem, Jumana Almuheimid, Rolf Richter, Elena Ioana Braicu, Suzan Osman, Jalid Sehouli
AIM: This study aimed to analyze diaphragmatic interventions and their complications in primary cytoreductive surgery (PCS) and to study their impact on attaining complete tumor resection (CTR) in advanced ovarian cancer (AOC), which is purportedly reflected in better disease-free and overall survival. PATIENTS AND METHODS: The study's collective consisted of 536 consecutive patients presenting a first diagnosis of AOC who underwent PCS between 2007 and 2013 at the Charité Medical University, Berlin...
September 2016: Anticancer Research
Erica C Dun, Serena Wong, Nisha A Lakhi, Ceana H Nehzat
OBJECTIVE: To report the medical and surgical management of a rare case of recurrent moss-like endometriosis and associated hemorrhagic ascites. DESIGN: Video description of the case, demonstration of the surgical technique, discussion of the histology, and review of endometriosis-associated ascites. SETTING: Tertiary referral center. PATIENT(S): A 26-year-old nulliparous woman of Nigerian heritage with recurrent hemorrhagic ascites due to endometriosis...
August 16, 2016: Fertility and Sterility
Jana Billson, F Haynes van der Merwe, Robbert P Soeters
INTRODUCTION: The aim of this study was to assess practice patterns and rationale with regard to debulking surgery for advanced epithelial ovarian carcinoma among South African Gynaecological oncologists. METHODS: A survey was distributed to all practicing gynecological oncologists in South Africa. It was structured to assess definitions of optimal debulking, procedures used to achieve optimal debulking, and reasons for not performing specific procedures. Respondents were also asked to report on experience and additional surgical training to improve skill in performing ultra-radical debulking surgery...
October 2016: International Journal of Gynecological Cancer
Cagdas Sahin, Ali Akdemir, Ahmet Mete Ergenoglu, Banu Ozgurel, Ahmet Ozgur Yeniel, Dilek Taskiran, Fatih Sendag
The aim of the present study was to determine the long-term effects of different laparoscopic hemostatic techniques on ovarian reserve after ovarian cystectomy. Ninety patients with unilateral ovarian cysts were recruited and randomly distributed into 2 groups. Laparoscopic stripping cystectomy was performed in all patients. Afterward, cystectomy hemostasis was achieved via hemostatic suture or bipolar electrocoagulation. Serum levels of anti-Müllerian hormone (AMH) were determined preoperatively and postoperatively at 1, 3, and 12 months, and patients were evaluated for residual ovarian volume, antral follicle count, and pregnancy...
July 19, 2016: Reproductive Sciences
Christina Jeanneret, Konstantin Beier, Alexander von Weymarn, Jürg Traber
Knowledge of the anatomy of the pelvic, gonadal and renal veins is important to understand pelvic congestion syndrome (PCS) and left renal vein compression syndrome (LRCS), which is also known as the nutcracker syndrome. LRCS is related to PCS and to the presence of vulvar, vaginal and pudendal varicose veins. The diagnosis of the two syndromes is difficult, and usually achieved with CT- or phlebography. The gold standard is the intravenous pressure measurement using conventional phlebography. The definition of PCS is described as pelvic pain, aggravated in the standing position and lasting for more than 6 months...
2016: VASA. Zeitschrift Für Gefässkrankheiten
Adel Shervin, Arash Mohazzab, Mina Aminlou, Koorosh Kamali, Roya Padmehr, Khadijeh Shadjoo, Parvin Jaberi-Pour, Elham Akbari
OBJECTIVE: To evaluate the result of laparoscopic endometrioma excision in fertility outcome of advanced endometriosis patients. STUDY DESIGN: The study was designated as historical cohort, in a private referral center of advance laparoscopy. 111 infertile patients, diagnosed as endometriosis, were divided in two groups: DIE (deep infiltrative endometriosis) and endometrioma (case group), and patients with only DIE (without endometrioma ((control group). All patients underwent global laparoscopic resection of DIE lesion (both groups) and laparoscopic excisional cystectomy of endometrioma (case groups)...
June 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Christina Fotopoulou, Benjamin P Jones, Konstantinos Savvatis, Jeremy Campbell, Maria Kyrgiou, Alan Farthing, Stephen Brett, Rene Roux, Marcia Hall, Gordon Rustin, Hani Gabra, Long Jiao, Richard Stümpfle
OBJECTIVE: To assess surgical morbidity and mortality of maximal effort cytoreductive surgery for disseminated epithelial ovarian cancer (EOC) in a UK tertiary center. METHODS/MATERIALS: A monocentric prospective analysis of surgical morbidity and mortality was performed for all consecutive EOC patients who underwent extensive cytoreductive surgery between 01/2013 and 12/2014. Surgical complexity was assessed by the Mayo clinic surgical complexity score (SCS). Only patients with high SCS ≥5 were included in the analysis...
September 2016: Archives of Gynecology and Obstetrics
Lia Cruz V C Damásio, José Maria Soares-Júnior, Jairo Iavelberg, Gustavo A R Maciel, Manuel de Jesus Simões, Ricardo Dos Santos Simões, Eduardo Vieira da Motta, Maria Cândida Pinheiro Baracat, Edmund C Baracat
BACKGROUND: Ovarian autotransplantation has shown increasing promise as a clinical method for the preservation of fertility and hormonal function. However, information regarding the success rate of this type of transplantation is limited. We hypothesized that results vary according to the site of the ovarian transplantation. To test this hypothesis, fresh or cryopreserved ovarian strips were autotransplanted to orthotopic or heterotopic sites. The strips were later collected, and the morphology and expression of selected markers of apoptosis were evaluated...
2016: Journal of Ovarian Research
Filiz Tepekoy, Gokhan Akkoyunlu
BACKGROUND: rhFSH and rhActA have been used in mammalian ovarian follicle culture systems for activation of follicular growth in vitro and suggested to be responsible for primordial follicle survival through MAPK and Akt pathways. The aim of our study was to determine the effects of rhFSH and rhActA on Akt, pAkt, MAPK1/3 and pMAPK1/3 protein levels in bovine ovarian cortical strips cultured in vitro. METHODS: Ovarian cortical strips from heifers were cultured in the presence of rhFSH (50 ng/mL), rhActA (100 ng/mL) or combination of these factors for 6 days...
2016: Journal of Ovarian Research
A Langbeen, C Van Ginneken, E Fransen, E Bosmans, J L M R Leroy, P E J Bols
The increasing number of cancer survivors the past decades, has sparked the need for fertility preservation strategies. Due to predominantly ethical constraints, human research material is scarce. A bovine in vitro model is a valuable alternative. Therefore, the following objectives were defined: 1) to xeno-graft bovine ovarian cortex tissue in immune deficient mice as a study-model for female fertility preservation strategies; 2) to stereologically quantify vascularization in Vascular Endothelial Growth Factor (VEGF)-treated and non-treated tissue; 3) to study preantral follicular survival in situ, after xenotransplantation...
May 2016: Animal Reproduction Science
Riccardo Talevi, Vincenza Barbato, Ilaria Fiorentino, Sabrina Braun, Cristofaro De Stefano, Raffaele Ferraro, Sam Sudhakaran, Roberto Gualtieri
OBJECTIVE: To study whether slush nitrogen vitrification improves the preservation of human ovarian tissue. DESIGN: Control vs. treatment study. SETTING: University research laboratory. PATIENT(S): Ovarian biopsies collected from nine women (aged 14-35 years) during laparoscopic surgery for benign gynecologic conditions. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Ovarian cortical strips of 2 × 5 × 1 mm were vitrified with liquid or slush nitrogen...
June 2016: Fertility and Sterility
Angelo Cagnacci, Manuela Bellafronte, Anjeza Xholli, Federica Palma, Maria Maddalena Carbone, Costantino Di Carlo, Giovanni Grandi
OBJECTIVE: To evaluate the effect on ovarian reserve and blood flow of unilateral laparoscopic stripping of endometriotic versus non-endometriotic cysts. DESIGN: Prospective observational study. SETTING: Tertiary university gynecology unit. PATIENTS: During the study period, 71 subjects underwent the first laparoscopic surgery for removal of a monolateral benign ovarian cyst. INTERVENTIONS: Trans-vaginal ultrasound scans of the pelvis about six months after surgery...
2016: Gynecological Endocrinology
Ludovico Muzii, Chiara Achilli, Valentino Bergamini, Massimo Candiani, Elisabetta Garavaglia, Lucia Lazzeri, Francesca Lecce, Antonio Maiorana, Francesco Maneschi, Riccardo Marana, Alessio Perandini, Maria Grazia Porpora, Renato Seracchioli, Emanuela Spagnolo, Michele Vignali, Pierluigi Benedetti Panici
STUDY QUESTION: Is the combined excisional/ablative technique for the treatment of ovarian endometriomas better than the traditional stripping technique in terms of recurrence rate? SUMMARY ANSWER: There is no evidence that the combined excisional/ablative technique is better than the traditional stripping technique, as similar recurrence rates were observed for the two techniques. WHAT IS KNOWN ALREADY: The stripping technique is associated with better results compared with ablative, non-excisional techniques for the treatment of ovarian endometriomas...
February 2016: Human Reproduction
C U Eze, H C Ugwu, C U Eze, K Ochie, I U Nwadike, C Otika
AIM: Accurate prediction of ovulation is important in the management of female infertility. The aim of this study was to determine the sonographic sensitivity of reduction in follicular size and disappearance of ovarian follicle as predictors of imminent ovulation. METHODS: This was a longitudinal study involving 100 women between the ages of 18 and 35 years. Transvaginal sonography with 6.5 MHz probe frequency was performed with a General Electric (RT 2800) ultrasound machine...
May 13, 2015: West Indian Medical Journal
Leila Sadat Tahaei, Hussein Eimani, Ghazaleh Hajmusa, Rouhollah Fathi, Mojtaba Rezazadeh Valojerdi, Abdolhossein Shahverdi, Poopak Eftekhari-Yazdi
BACKGROUND: This study aimed to assess follicle survival after xenotransplantation of sheep ovarian tissue into male and female immunodeficient rats. We evaluated the effects of gonadotropin treatment on follicular development in the transplanted tissue. MATERIALS AND METHODS: In this experimental study, sheep ovarian cortical strips were transplanted into the neck back muscles of 8 male and 8 female immunodeficient, castrated rats. Fourteen days after surgery, each rat was treated with human menopausal gonadotropin (hMG) for 9 weeks...
October 2015: International Journal of Fertility & Sterility
Julien Seror, Anne-Sophie Bats, Habib Habchi, Fabrice Lécuru
OBJECTIVE: To show the contribution of PlasmaJet™, technology using pur gaz plasma as electrically neutral source of energy to cut and coagulate tissue, in ovarian cancer surgery. METHODS: A forty eight years old patient was diagnosed with an ovarian cancer. The first laparoscopy allowed one to make histological diagnosis (ovarian high-grade serous carcinoma) and determine that the disease was resecable, with a PCI to ten. The film shows the upper abdominal time of the cytoreduction surgery, using PlasmaJet™...
February 2016: Gynecologic Oncology
Giorgio Bogani, Antonino Ditto, Fabio Martinelli, Domenica Lorusso, Valentina Chiappa, Cristina Donfrancesco, Violante Di Donato, Alice Indini, Giovanni Aletti, Francesco Raspagliesi
OBJECTIVE: Optimal cytoreduction is one the main factors improving survival outcomes in patients affected by ovarian cancer (OC). It is estimated that approximately 40% of OC patients have gross disease located on the diaphragm. However, no mature data evaluating outcomes of surgical techniques for the management of diaphragmatic carcinosis exist. In the present study, we aimed to estimate surgery-related morbidity of different surgical techniques for diaphragmatic cytoreduction in advanced or recurrent OC...
February 2016: International Journal of Gynecological Cancer
Ming-Jun Shao, Min Hu, Yun-Qin He, Xiao-Jiu Xu
PURPOSE: To evaluate the ovarian reserve after laparoscopic cystectomy with suturing in patients with endometriomas. METHODS: A total of 80 women with unilateral or bilateral endometriomas underwent laparoscopic cystectomy using sutures for hemostasis after the excision of ovarian cysts. Serum levels of antimullerian hormone (AMH) and FSH were measured at the day 3 of menstrual cycles preoperatively, 6 and 12 months postoperatively. RESULTS: In the bilateral endometrioma group, serum AMH level decreased significantly from the baseline (4...
May 2016: Archives of Gynecology and Obstetrics
Zahra Asgari, Safoura Rouholamin, Reihaneh Hosseini, Mahdi Sepidarkish, Leila Hafizi, Atiyeh Javaheri
PURPOSE: This study aimed to compare ovarian reserve between laparoscopic suturing and bipolar coagulation techniques in women with unilateral endometrioma. METHODS: In a prospective randomized clinical trial, 109 patients with unilateral endometrioma underwent laparoscopic cystectomy. Patients were then randomized to undergo hemostasis with either bipolar coagulation (n = 57) or suturing (n = 52) technique. We evaluated the impact of surgery and hemostasis techniques on ovarian reserve using serum levels of anti-Mullerian hormone (AMH) and follicle-stimulating hormone (FSH) that were measured preoperatively and at 3 months postoperatively...
May 2016: Archives of Gynecology and Obstetrics
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