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https://www.readbyqxmd.com/read/27836611/a-sers-nano-tag-based-fiber-optic-strategy-for-in-situ-immunoassay-in-unprocessed-whole-blood
#1
Xiaokun Li, Youlin Zhang, Bin Xue, Xianggui Kong, Xiaomin Liu, Langping Tu, Yulei Chang, Hong Zhang
Assay technologies capable of detecting biomarker concentrations in unprocessed whole blood samples are fundamental for applications in medical diagnostics. SERS nano-tags integrated fiber-optic biosensor (FOB) was realized for the first time for in situ immunoassay in whole blood. The reliability and sensitivity of this method rely, in a large extent, on the quality and properties of the SERS nano-tags. The constructed silica-coated Ag SERS nano-tags as labels were used in a rapid and specific in situ FOB immune sensor to detect alpha fetoprotein (AFP) in unprocessed blood samples...
October 28, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27692902/dnm3-and-genetic-modifiers-of-age-of-onset-in-lrrk2-gly2019ser-parkinsonism-a-genome-wide-linkage-and-association-study
#2
Joanne Trinh, Emil K Gustavsson, Carles Vilariño-Güell, Stephanie Bortnick, Jeanne Latourelle, Marna B McKenzie, Chelsea Szu Tu, Ekaterina Nosova, Jaskaran Khinda, Austen Milnerwood, Suzanne Lesage, Alexis Brice, Meriem Tazir, Jan O Aasly, Laura Parkkinen, Hazal Haytural, Tatiana Foroud, Richard H Myers, Samia Ben Sassi, Emna Hentati, Fatma Nabli, Emna Farhat, Rim Amouri, Fayçal Hentati, Matthew J Farrer
BACKGROUND: Leucine-rich repeat kinase 2 (LRRK2) mutation 6055G→A (Gly2019Ser) accounts for roughly 1% of patients with Parkinson's disease in white populations, 13-30% in Ashkenazi Jewish populations, and 30-40% in North African Arab-Berber populations, although age of onset is variable. Some carriers have early-onset parkinsonism, whereas others remain asymptomatic despite advanced age. We aimed to use a genome-wide approach to identify genetic variability that directly affects LRRK2 Gly2019Ser penetrance...
November 2016: Lancet Neurology
https://www.readbyqxmd.com/read/27576884/the-prevalence-and-prognostic-significance-of-polyomavirus-infection-in-patients-with-urothelial-carcinoma-of-the-bladder
#3
Sheng-Wen Wu, Jia-Hung Liou, Kun-Tu Yeh, Tung-Wei Hung, Horng-Rong Chang
PURPOSE: Human polyomaviruses (PV) has been associated with oncogenicity; however, the association between human bladder cancer and PV remains inconclusive. Moreover, whether PV has the interaction with p53 in tumorigenesis and their prognostic significance on human bladder cancer has yet to be determined. MATERIALS AND METHODS: Bladder tumor specimens and clinical parameters from 74 patients with urothelial carcinoma were collected. Immunohistochemical analysis using monoclonal antibodies specific to PV large tumor antigen (TAg) and p53 protein was performed to investigate the involvement of PV in human bladder tumorigenesis and the prognostic significance of TAg and p53 expressions using Cox proportional hazards model...
2016: Urology Journal
https://www.readbyqxmd.com/read/27443933/cell-type-specific-transcriptomic-analysis-by-thiouracil-tagging-in-zebrafish
#4
T Erickson, T Nicolson
Transcriptomic studies are important tools for understanding the development and function of the different cell types that make up complex tissues. Zebrafish (Danio rerio) is a valuable organism for modeling key aspects of vertebrate development, cell biology, and human disease. However, the small size of individual larvae and relative scarcity of certain cell types in zebrafish can hamper efforts to collect enough pure material for cell type-specific transcriptomic studies. Thus, there is a need in the zebrafish field for spatially and temporally resolved gene expression assays...
2016: Methods in Cell Biology
https://www.readbyqxmd.com/read/27440201/msvolcano-a-flexible-web-application-for-visualizing-quantitative-proteomics-data
#5
Sukhdeep Singh, Marco Y Hein, A Francis Stewart
: We introduce msVolcano, a web application for the visualization of label-free mass spectrometric data. It is optimized for the output of the MaxQuant data analysis pipeline of interactomics experiments and generates volcano plots with lists of interacting proteins. The user can optimize the cutoff values to find meaningful significant interactors for the tagged protein of interest. Optionally, stoichiometries of interacting proteins can be calculated. Several customization options are provided to the user for flexibility, and publication-quality outputs can also be downloaded (tabular and graphical)...
September 2016: Proteomics
https://www.readbyqxmd.com/read/27379103/transient-expression-of-tetrameric-recombinant-human-butyrylcholinesterase-in-nicotiana-benthamiana
#6
Salem Alkanaimsh, Kalimuthu Karuppanan, Andrés Guerrero, Aye M Tu, Bryce Hashimoto, Min Sook Hwang, My L Phu, Lucas Arzola, Carlito B Lebrilla, Abhaya M Dandekar, Bryce W Falk, Somen Nandi, Raymond L Rodriguez, Karen A McDonald
To optimize the expression, extraction and purification of plant-derived tetrameric recombinant human butyrylcholinesterase (prBChE), we describe the development and use of plant viral amplicon-based gene expression system; Tobacco Mosaic Virus (TMV) RNA-based overexpression vector (TRBO) to express enzymatically active FLAG-tagged plant made recombinant butyrylcholinesterase (rBChE) in Nicotiana benthamiana leaves using transient agroinfiltration. Two gene expression cassettes were designed to express the recombinant protein in either the ER or to the apoplastic compartment...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27240439/a-rapid-and-efficient-method-to-study-the-function-of-crop-plant-transporters-in-arabidopsis
#7
Xiangfeng Wang, Fudi Zhong, Cheuk Hang Woo, Yansong Miao, Michael A Grusak, Xiaobo Zhang, Jumin Tu, Yum Shing Wong, Liwen Jiang
Iron (Fe) is an essential micronutrient for humans. Fe deficiency disease is widespread and has led to extensive studies on the mechanisms of Fe uptake and storage, especially in staple food crops such as rice. However, studies of functionally related genes in rice and other crops are often time and space demanding. Here, we demonstrate that transgenic Arabidopsis suspension culture cells and Arabidopsis plants can be used as an efficient expression system for gain-of-function study of selected transporters, using Fe transporters as a proof-of-principle...
May 30, 2016: Protoplasma
https://www.readbyqxmd.com/read/27091628/%C3%AE-synuclein-genetic-variability-a-biomarker-for-dementia-in-parkinson-disease
#8
Ilaria Guella, Daniel M Evans, Chelsea Szu-Tu, Ekaterina Nosova, Stephanie F Bortnick, Jennifer G Goldman, John C Dalrymple-Alford, Gert J Geurtsen, Irene Litvan, Owen A Ross, Lefkos T Middleton, Laura Parkkinen, Matthew J Farrer
OBJECTIVE: The relationship between Parkinson disease (PD), PD with dementia (PDD), and dementia with Lewy bodies (DLB) has long been debated. Although PD is primarily considered a motor disorder, cognitive impairment is often present at diagnosis, and only ∼20% of patients remain cognitively intact in the long term. Alpha-synuclein (SNCA) was first implicated in the pathogenesis of the disease when point mutations and locus multiplications were identified in familial parkinsonism with dementia...
June 2016: Annals of Neurology
https://www.readbyqxmd.com/read/27027731/caloric-restriction-coupled-with-radiation-decreases-metastatic-burden-in-triple-negative-breast-cancer
#9
Brittany A Simone, Tu Dan, Ajay Palagani, Lianjin Jin, Sunny Y Han, Christopher Wright, Jason E Savage, Robert Gitman, Meng Kieng Lim, Juan Palazzo, Minesh P Mehta, Nicole L Simone
PURPOSE: Metastatic breast cancer is devastating and triple negative breast cancers (TNBC) have a higher propensity for metastasis. Improved local control upfront in this aggressive cancer could potentially decrease its propensity toward metastasis. We sought to determine if using caloric restriction (CR) as a systemic therapy, combined with radiation therapy (IR) to the primary tumor, may impact metastatic disease. METHODS: An orthotopic mouse model using a highly metastatic, luciferase-tagged TNBC cell line (4T1), was used to generate palpable tumors...
September 2016: Cell Cycle
https://www.readbyqxmd.com/read/27011954/transcriptional-profiling-of-newly-generated-dentate-granule-cells-using-tu-tagging-reveals-pattern-shifts-in-gene-expression-during-circuit-integration
#10
Christina Chatzi, Yingyu Zhang, Rongkun Shen, Gary L Westbrook, Richard H Goodman
Despite representing only a small fraction of hippocampal granule cells, adult-generated newborn granule cells have been implicated in learning and memory (Aimone et al., 2011). Newborn granule cells undergo functional maturation and circuit integration over a period of weeks. However, it is difficult to assess the accompanying gene expression profiles in vivo with high spatial and temporal resolution using traditional methods. Here we used a novel method ["thiouracil (TU) tagging"] to map the profiles of nascent mRNAs in mouse immature newborn granule cells compared with mature granule cells...
January 2016: ENeuro
https://www.readbyqxmd.com/read/26863113/multidentate-polymer-coatings-for-compact-and-homogeneous-quantum-dots-with-efficient-bioconjugation
#11
Liang Ma, Chunlai Tu, Phuong Le, Shweta Chitoor, Sung Jun Lim, Mohammad U Zahid, Kai Wen Teng, Pinghua Ge, Paul R Selvin, Andrew M Smith
Quantum dots are fluorescent nanoparticles used to detect and image proteins and nucleic acids. Compared with organic dyes and fluorescent proteins, these nanocrystals have enhanced brightness, photostability, and wavelength tunability, but their larger size limits their use. Recently, multidentate polymer coatings have yielded stable quantum dots with small hydrodynamic dimensions (≤10 nm) due to high-affinity, compact wrapping around the nanocrystal. However, this coating technology has not been widely adopted because the resulting particles are frequently heterogeneous and clustered, and conjugation to biological molecules is difficult to control...
March 16, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/26829740/-cytogenetic-and-molecular-genetic-analysis-of-a-case-with-mosaic-marker-chromosomes
#12
Xiangdong Tu, Jian Zeng, Xuewen Cong, Xiao Zhang, Aizhen Yan
OBJECTIVE: To explore the source of small supernumerary marker chromosome in a case. METHODS: G-banded karyotyping, fluorescence in situ hybridization, multiple sequence tagged sites (STS) of the Y chromosome, and Illumima Human Cyto SNP-12 Beadchip analysis were carried out. RESULTS: The karyotype was mos 46,X,+mar1[21]/46,X,+mar2[78]. Y chromosome STS analysis has displayed the presence of sy84, sY86, USP9Y and DDX3Y genes from the AZFa region, and sY1227 of the AZFb region, while sY1228, sY1015, sY127, sY134 from the AZFb region, and sY254 and sY255 from the AZFc region were missing...
February 2016: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/26494580/identification-of-sensory-hair-cell-transcripts-by-thiouracil-tagging-in-zebrafish
#13
Timothy Erickson, Teresa Nicolson
BACKGROUND: Sensory hair cells are exquisitely sensitive to mechanical stimuli and as such, are prone to damage and apoptosis during dissections or in vitro manipulations. Thiouracil (TU)-tagging is a noninvasive method to label cell type-specific transcripts in an intact organism, thereby meeting the challenge of how to analyze gene expression in hair cells without the need to sort cells. We adapted TU-tagging to zebrafish to identify novel transcripts expressed in the sensory hair cells of the developing acoustico-lateralis organs...
2015: BMC Genomics
https://www.readbyqxmd.com/read/26450559/a-facile-and-general-route-to-synthesize-silica-coated-sers-tags-with-the-enhanced-signal-intensity
#14
Youlin Zhang, Xiaokun Li, Bin Xue, Xianggui Kong, Xiaomin Liu, Langping Tu, Yulei Chang
Silica-coated SERS tags have been attracting greater attention in recent years. However, the reported methods to synthesize these tags are tedious, and often subjected to the limited signal intensity. Here, we report a facile and general method to prepare the silica-coated Ag SERS tags with the enhanced signal intensity by no introducing the primers. This approach mainly depends on the colloidal stability of the Ag NPs in alcohol solution. By decreasing the concentration of salt in Ag NP solution, the citrate-stabilized Ag NPs can be well dispersed in alcohol solution...
2015: Scientific Reports
https://www.readbyqxmd.com/read/26439030/microarray-based-analysis-of-gene-expression-patterns-in-pancreatic-neuroendocrine-tumors
#15
D-D Wang, Z-W Liu, M-M Han, Z-M Zhu, Y-L Tu, C-Q Dou, X Jin, J Xin, S-W Cai, C-S Wang, N Du, D Nan
OBJECTIVE: Pancreatic neuroendocrine tumors (PanNETs) are a small subgroup of tumors with a variety of biological behaviors. MATERIALS AND METHODS: We sought to identify the specially expressed genes and characterize significant pathways in PanNETs compared with non-neoplastic samples. Gene expression profile GSE43795 was obtained from Gene Expression Omnibus database, which included 6 PanNETs and 5 non-neoplastic samples. The differentially expressed genes (DEGs) were identified using Limma package...
September 2015: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/26378254/conserved-function-of-the-lysine-based-kxd-e-motif-in-golgi-retention-for-endomembrane-proteins-among-different-organisms
#16
Cheuk Hang Woo, Caiji Gao, Ping Yu, Linna Tu, Zhaoyue Meng, David K Banfield, Xiaoqiang Yao, Liwen Jiang
We recently identified a new COPI-interacting KXD/E motif in the C-terminal cytosolic tail (CT) of Arabidopsis endomembrane protein 12 (AtEMP12) as being a crucial Golgi retention mechanism for AtEMP12. This KXD/E motif is conserved in CTs of all EMPs found in plants, yeast, and humans and is also present in hundreds of other membrane proteins. Here, by cloning selective EMP isoforms from plants, yeast, and mammals, we study the localizations of EMPs in different expression systems, since there are contradictory reports on the localizations of EMPs...
November 15, 2015: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/26371515/splicing-factor-prp8-interacts-with-nes-ar-and-regulates-androgen-receptor-in-prostate-cancer-cells
#17
Dan Wang, Minh M Nguyen, Khalid Z Masoodi, Prabhpreet Singh, Yifeng Jing, Katherine O'Malley, Javid A Dar, Rajiv Dhir, Zhou Wang
Androgen receptor (AR) plays a pivotal role in the development of primary as well as advanced castration-resistant prostate cancer. Previous work in our lab identified a novel nuclear export signal (NES) (NES(AR)) in AR ligand-binding domain essential for AR nucleocytoplasmic trafficking. By characterizing the localization of green fluorescence protein (GFP)-tagged NES(AR), we designed and executed a yeast mutagenesis screen and isolated 7 yeast mutants that failed to display the NES(AR) export function. One of those mutants was identified as the splicing factor pre-mRNA processing factor 8 (Prp8)...
December 2015: Molecular Endocrinology
https://www.readbyqxmd.com/read/26365175/genetic-variants-of-the-autophagy-pathway-as-prognostic-indicators-for-prostate-cancer
#18
Chao-Yuan Huang, Shu-Pin Huang, Victor C Lin, Chia-Cheng Yu, Ta-Yuan Chang, Te-Ling Lu, Hung-Chih Chiang, Bo-Ying Bao
Autophagy is a complex process of autodigestion in conditions of cellular stress, and it might play an important role in the pathophysiology during carcinogenesis. We hypothesize that genetic variants of the autophagy pathway may influence clinical outcomes in prostate cancer patients. We genotyped 40 tagging single-nucleotide polymorphisms (SNPs) from 7 core autophagy pathway genes in 458 localized prostate cancer patients. Multivariate Cox regression was performed to evaluate the independent association of each SNP with disease progression...
September 14, 2015: Scientific Reports
https://www.readbyqxmd.com/read/26360102/a-metal-carbonyl-protein-needle-composite-designed-for-intracellular-co-delivery-to-modulate-nf-%C3%AE%C2%BAb-activity
#19
Hiroshi Inaba, Nusrat J M Sanghamitra, Kenta Fujita, Takeya Sho, Takahiro Kuchimaru, Susumu Kitagawa, Shinae Kizaka-Kondoh, Takafumi Ueno
Carbon monoxide (CO) has been recognized as a messenger for signal transduction in living cells and tissues. For intracellular CO delivery, several metal carbonyl complexes have been used as CO-releasing molecules (CO-RMs). To improve the properties of CO-RMs, such as the stability and the CO release rate, ligands and carriers of the metal complexes have been exploited. Here we report the development of an efficient intracellular CO delivery system using a protein scaffold. We used a protein needle reconstructed from gene product 5 of bacteriophage T4, which has high cellular permeability and stability...
November 2015: Molecular BioSystems
https://www.readbyqxmd.com/read/26359451/cancer-stem-cell-marker-phenotypes-are-reversible-and-functionally-homogeneous-in-a-preclinical-model-of-pancreatic-cancer
#20
Joseph S Dosch, Elizabeth K Ziemke, Amrith Shettigar, Alnawaz Rehemtulla, Judith S Sebolt-Leopold
Survival rates associated with pancreatic cancer remain dismal despite advancements in detection and experimental treatment strategies. Genetically engineered mouse models of pancreatic tumorigenesis have gained considerable attention based on their ability to recapitulate key clinical features of human disease including chemotherapeutic resistance and fibrosis. However, it is unclear if transgenic systems exemplified by the Kras(G12D)/Trp53(R172H)/Pdx-1-Cre (KPC) mouse model recapitulate the functional heterogeneity of human pancreatic tumors harboring distinct cells with tumorigenic properties...
November 1, 2015: Cancer Research
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