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https://www.readbyqxmd.com/read/28732263/proteomic-analysis-of-naturally-occurring-boron-tolerant-plant-gypsophila-sphaerocephala-l-in-response-to-high-boron-concentration
#1
Huseyin Tombuloglu, Guzin Tombuloglu, Mehmet Serdal Sakcali, Ali Turkan, Khalid Rehman Hakeem, Hesham F Alharby, Shah Fahd, Waseem Mohammed Abdul
Gypsophila sphaerocephala is a naturally Boron (B) tolerant species that can grow around the B mining areas in Turkey, where the B concentration in soil reaches a lethal dose for plants (up to ∼8900mgkg(-1) (∼140mM). While its interesting survival capacity in extremely B containing soils, any molecular research has been conducted to understand its high tolerance mechanism yet. In the present study, we have performed a proteomic analysis of this plant to understand its high tolerance towards B-stress. Seedlings of G...
July 4, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28732077/mutations-in-the-caenorhabditis-elegans-orthologs-of-human-genes-required-for-mitochondrial-trna-modification-cause-similar-electron-transport-chain-defects-but-different-nuclear-responses
#2
Carmen Navarro-González, Ismaïl Moukadiri, Magda Villarroya, Ernesto López-Pascual, Simon Tuck, M-Eugenia Armengod
Several oxidative phosphorylation (OXPHOS) diseases are caused by defects in the post-transcriptional modification of mitochondrial tRNAs (mt-tRNAs). Mutations in MTO1 or GTPBP3 impair the modification of the wobble uridine at position 5 of the pyrimidine ring and cause heart failure. Mutations in TRMU affect modification at position 2 and cause liver disease. Presently, the molecular basis of the diseases and why mutations in the different genes lead to such different clinical symptoms is poorly understood...
July 21, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28730731/induction-of-stress-and-immune-associated-genes-in-the-indian-meal-moth-plodia-interpunctella-against-envenomation-by-the-ectoparasitoid-bracon-hebetor
#3
Tahir Shafeeq, Zain UlAbdin, Kyeong-Yeoll Lee
Envenomation is an important process in parasitism by parasitic wasps; it suppresses the immune and development of host insects. However, the molecular mechanisms of host responses to envenomation are not yet clear. This study aimed to determine the transcription-level responses of the Indian meal moth Plodia interpunctella against envenomation of the ectoparasitoid Bracon hebetor. Quantitative real-time reverse-transcription PCR was used to determine the transcriptional changes of 13 selected genes, which are associated with development, metabolism, stress, or immunity, in the feeding and wandering fifth instar larvae over a 4-day period after envenomation...
July 21, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28730560/impairment-of-ntca-gene-revealed-its-role-in-regulating-iron-homeostasis-ros-production-and-cellular-phenotype-under-iron-deficiency-in-cyanobacterium-anabaena-sp-pcc-7120
#4
Manish Singh Kaushik, Meenakshi Srivastava, Anumeha Singh, Arun Kumar Mishra
Iron deficiency ends up into several unavoidable consequences including damaging oxidative stress in cyanobacteria. NtcA is a global nitrogen regulator controls wide range of metabolisms in addition to regulation of nitrogen metabolism. In present communication, NtcA based regulation of iron homeostasis, ROS production and cellular phenotype under iron deficiency in Anabaena 7120 has been investigated. NtcA regulates the concentration dependent iron uptake by controlling the expression of furA gene. NtcA also regulated pigment synthesis and phenotypic alterations in Anabaena 7120...
August 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28730540/gigaspora-margarita-with-and-without-its-endobacterium-shows-adaptive-responses-to-oxidative-stress
#5
Francesco Venice, Maria Concetta de Pinto, Mara Novero, Stefano Ghignone, Alessandra Salvioli, Paola Bonfante
Arbuscular mycorrhizal (AM) fungi experience oxidative stress during the plant-fungal interaction, due to endogenous reactive oxygen species (ROS) produced by fungal metabolism and exogenous ROS produced by plant cells. Here, we examine the responses to H2O2 in Gigaspora margarita, an AM fungus containing the endobacterial symbiont Candidatus Glomeribacter gigasporarum (CaGg). Previous studies revealed that G. margarita with its endobacterium produces more ATP and has higher respiratory activity than a cured line that lacks the endobacterium...
July 20, 2017: Mycorrhiza
https://www.readbyqxmd.com/read/28730324/the-role-of-ndgr-in-glycerol-metabolism-in-streptomyces-coelicolor
#6
Bo-Rahm Lee, Shashi Kant Bhatia, Hun-Suk Song, Junyoung Kim, Wooseong Kim, Hyung-Yeon Park, Jeong-Jun Yoon, Sung-Hee Park, Daehee Hwang, Byung-Gee Kim, Yung-Hun Yang
Streptomyces, which produces many pharmaceutical antibiotics and anticancer agents, is a genus of soil-dwelling bacteria with numerous regulators that control both primary and secondary metabolism. NdgR is highly conserved in Streptomyces spp. and is known to be involved in antibiotic production, tolerance against shock and physical stress, nitrogen metabolism, leucine metabolism, and N-acetylglucosamine metabolism. As another function of NdgR, we report the involvement of NdgR in glycerol metabolism in S. coelicolor...
July 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28729917/autophagy-endoplasmic-reticulum-stress-and-the-unfolded-protein-response-in-intracerebral-hemorrhage
#7
Mingming Niu, Xiaohong Dai, Wei Zou, Xueping Yu, Wei Teng, Qiuxin Chen, Xiaowei Sun, Weiwei Yu, Huihui Ma, Peng Liu
Intracerebral hemorrhage (ICH) is a subtype of stroke that is followed by primary and secondary brain injury. As a result of the injury, cell metabolism is disrupted and a series of stress responses are activated, such as endoplasmic reticulum (ER) stress and the unfolded protein response (UPR), leading to the re-establishment of cell homeostasis or cell death. As an important mechanism of cell homeostasis, autophagy has been widely studied, and the associations between autophagy, ER stress, and the UPR have also been demonstrated...
2017: Translational Neuroscience
https://www.readbyqxmd.com/read/28729898/draft-genome-sequences-of-strains-salinicola-socius-smb35-t-salinicola-sp-mh3r3-1-and-chromohalobacter-sp-smb17-from-the-verkhnekamsk-potash-mining-region-of-russia
#8
Björn E Olsson, Ekaterina S Korsakova, Lyudmila N Anan'ina, Anna A Pyankova, Olga V Mavrodi, Elena G Plotnikova, Dmitri V Mavrodi
Halomonads are moderately halophilic bacteria that are studied as models of prokaryotic osmoadaptation and sources of enzymes and chemicals for biotechnological applications. Despite the progress in understanding the diversity of these organisms, our ability to explain ecological, metabolic, and biochemical traits of halomonads at the genomic sequence level remains limited. This study addresses this gap by presenting draft genomes of Salinicola socius SMB35(T), Salinicola sp. MH3R3-1 and Chromohalobacter sp...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28729539/comparative-proteomic-analysis-of-human-embryonic-stem-cell-derived-and-primary-human-retinal-pigment-epithelium
#9
Heidi Hongisto, Antti Jylhä, Janika Nättinen, Jochen Rieck, Tanja Ilmarinen, Zoltán Veréb, Ulla Aapola, Roger Beuerman, Goran Petrovski, Hannu Uusitalo, Heli Skottman
Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) provide an unlimited cell source for retinal cell replacement therapies. Clinical trials using hESC-RPE to treat diseases such as age-related macular degeneration (AMD) are currently underway. Human ESC-RPE cells have been thoroughly characterized at the gene level but their protein expression profile has not been studied at larger scale. In this study, proteomic analysis was used to compare hESC-RPE cells differentiated from two independent hESC lines, to primary human RPE (hRPE) using Isobaric tags for relative quantitation (iTRAQ)...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729344/exogenous-stress-hormones-alter-energetic-and-nutrient-costs-of-development-and-metamorphosis
#10
Lucas J Kirschman, Marshall D McCue, Justin G Boyles, Robin W Warne
Variation in environmental conditions during larval life stages can shape development during critical windows and have lasting effects on the adult organism. Changes in larval developmental rates in response to environmental conditions, for example, can trade-off with growth to determine body size and condition at metamorphosis, which can affect adult survival and fecundity. However, it is unclear how use of energy and nutrients shape trade-offs across life stage transitions because no studies have quantified these costs of larval development and metamorphosis...
July 20, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28729247/adaptive-regulation-of-pancreatic-acinar-mitochondrial-thiamin-pyrophosphate-uptake-process-possible-involvement-of-epigenetic-mechanism-s
#11
Subrata Sabui, Veedamali S Subramanian, Rubina Kapadia, Hamid M Said
The essentiality of thiamin stems from its roles as a co-factor (mainly in the form of thiamin pyrophosphate, TPP) in critical metabolic reactions including oxidative energy metabolism and reduction of cellular oxidative stress. Like other mammalian cells, pancreatic acinar cells (PAC) obtain thiamin from their surroundings and convert it to TPP; mitochondria then take up TPP by a carrier-mediated process that involves the mitochondrial TPP (MTPP) transporter (MTPPT; product of SLC25A19 gene). Previous studies have characterized different physiological/biological aspects of the MTPP uptake process, but little is known about its possible adaptive-regulation...
July 20, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28728928/gtf-231-a-mixture-of-gymnemic-acid-trigonelline-and-ferulic-acid-significantly-ameliorates-oxidative-stress-in-experimental-type-2-diabetes-in-rats
#12
Roshana Devi Vellai, Sharmila Chandiran, Subramanian Sorimuthu Pillai
OBJECTIVES: According to a report by the United States National Health and Nutrition Examination Survey, nearly 60% of patients with diabetes have more than one complication caused by chronic diabetes. Type 2 diabetes is a multifactorial metabolic disorder that accounts for more than 95% of people with diabetes and usually requires multitargeted therapeutic interventions. It arises due to insufficient insulin secretion or its action or both. Recently, we reported that the oral administration of gymnemic acid, trigonelline and ferulic acid in the ratio of 2:3:1 (GTF-231), a mixture of 3 phytoingredients at a concentration of 300 mg/kg body weight significantly improved the glucose homeostasis in rats with low-dose streptozotocin-induced type 2 diabetes that were fed with high-fat diet...
July 17, 2017: Canadian Journal of Diabetes
https://www.readbyqxmd.com/read/28728402/influence-of-body-condition-score-at-calving-on-the-metabolic-status-and-production-performance-of-murrah-buffaloes-bubalus-bubalis-during-the-transition-period
#13
Nelson Carvalho Delfino, Lucas Fialho de Aragão Bulcão, Henry Daniel Ruiz Alba, Mauricio Xavier da Silva Oliveira, Filipe Pinheiro Soares de Queiroz, Gleidson Giordano Pinto de Carvalho, Francisco Palma Rennó, José Esler Freitas Júnior
Objective: The purpose of this study was to evaluate the influence of body condition score (BCS) at calving on the metabolic status of female Murrah buffaloes in the transition period. Methods: Thirty-seven pregnant buffaloes (multiparous) were selected and monitored during the transition period based on their body condition score and on the estimated calving date. Two groups were formed: 1) buffaloes with a BCS > 3.5 (n = 17); this group was classified and named 'high BCS at calving' (HBCS); and 2) buffaloes with a BCS ≤ 3...
July 17, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28727939/phytohormone-and-genome-variations-in-vitis-amurensis-resistant-to-downy-mildew
#14
Ling Yin, Junjie Qu, Shuhan Deng, Shaoli Liu, Jiang Lu, Yali Zhang
Downy mildew (DM) resistance is a highly desirable agronomic trait in grape breeding. High variation in Plasmopara viticola resistance was found in Vitis cultivars. Some accessions show high P. viticola resistance even under conditions highly conducive to DM. Here, leaf disc inoculation experiments revealed that Vitis amurensis 'Zuoshaner' exhibited DM resistance with necrotic spots, whereas the V. amurensis × V. vinifera hybrid cultivar 'Zuoyouhong' was susceptible. Changes in plant hormones accumulation profiles, differed between the cultivars...
July 20, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28727777/8-oxoguanine-dna-glycosylase-ogg1-deficiency-elicits-coordinated-changes-in-lipid-and-mitochondrial-metabolism-in-muscle
#15
Vladimir Vartanian, Jana Tumova, Pawel Dobrzyn, Agnieszka Dobrzyn, Yusaku Nakabeppu, R Stephen Lloyd, Harini Sampath
Oxidative stress resulting from endogenous and exogenous sources causes damage to cellular components, including genomic and mitochondrial DNA. Oxidative DNA damage is primarily repaired via the base excision repair pathway that is initiated by DNA glycosylases. 8-oxoguanine DNA glycosylase (OGG1) recognizes and cleaves oxidized and ring-fragmented purines, including 8-oxoguanine, the most commonly formed oxidative DNA lesion. Mice lacking the OGG1 gene product are prone to multiple features of the metabolic syndrome, including high-fat diet-induced obesity, hepatic steatosis, and insulin resistance...
2017: PloS One
https://www.readbyqxmd.com/read/28727492/chronic-psychological-stress-seems-associated-with-elements-of-the-metabolic-syndrome-in-patients-with-ischaemic-heart-disease
#16
Natasha Bergmann, Søren Ballegaard, Jesper Krogh, Per Bech, Åke Hjalmarson, Finn Gyntelberg, Jens Faber
BACKGROUND AND OBJECTIVES: Chronic psychological stress, the metabolic syndrome (MS) and ischaemic heart disease (IHD) seem closely connected. In this study, we evaluate the association between chronic stress and elements of MS in patients with stable IHD. DESIGN: Cross-sectional cohort study. METHODS: Three hundred and fifty patients with stable IHD were included. Chronic stress was evaluated by the two questionnaires, Major Depression Inventory (MDI) and the psychological wellbeing index WHO-5, as well as by Pressure Pain Sensitivity (PPS), a physiological measure of hyperalgesia at the sternum known to be associated to elements of the chronic stress syndrome...
July 20, 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28727232/functional-genomic-analysis-of-corals-from-natural-co2-seeps-reveals-core-molecular-responses-involved-in-acclimatization-to-ocean-acidification
#17
C D Kenkel, A Moya, J Strahl, C Humphrey, L K Bay
Little is known about the potential for acclimatization or adaptation of corals to ocean acidification and even less about the molecular mechanisms underpinning these processes. Here we examine global gene expression patterns in corals and their intracellular algal symbionts from two replicate population pairs in Papua New Guinea that have undergone long-term acclimatization to natural variation in pCO2 . In the coral host, only 61 genes were differentially expressed in response to pCO2 environment, but the pattern of change was highly consistent between replicate populations, likely reflecting the core expression homeostasis response to ocean acidification...
July 20, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28727118/technical-note-assessment-of-an-alternative-technique-for-measuring-body-temperature-in-pigs
#18
A Petry, W McGilvray, A R Rakhshandeh, A Rakhshandeh
Core body temperature (CBT) is one of the main vital signs that is used to evaluate the health status of pigs. The most common and feasible method for assessing CBT in pigs is rectal temperature (RT). Obtaining RT is stressful for animals, may generate inaccurate results, and has the risk of spreading disease. Infrared imaging (IR) thermography of the body of pigs may be a safer and less stressful alternative to RT. The objective of the current study was to evaluate the efficacy of using IR as an alternative for monitoring CBT in pigs...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28727041/proteomic-features-linked-to-tenderness-of-aged-pork-loins
#19
K B Carlson, K J Prusa, C A Fedler, E M Steadham, E Huff-Lonergan, S M Lonergan
There is considerable evidence that the protein component of fresh pork makes a major contribution to tenderness. In particular, the proteomic profile can be linked to postmortem events including pH decline, tissue oxidation, and protein degradation. The objectives for this study were to determine differences in sarcoplasmic proteomes that contribute to tenderness variation in aged pork longissimus dorsi muscles (LM). A defined set of pork loins selected to be similar in pH, color, and lipid yet different in tenderness were used...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28727025/effect-of-handling-intensity-at-the-time-of-transport-for-slaughter-on-physiological-response-and-carcass-characteristics-in-beef-cattle-fed-ractopamine-hydrochloride
#20
J A Hagenmaier, C D Reinhardt, S J Bartle, J N Henningson, M J Ritter, M S Calvo-Lorenzo, G J Vogel, C A Guthrie, M G Siemens, D U Thomson
The effects of handling intensity on the physiological response and carcass characteristics of feedlot cattle fed ractopamine hydrochloride were evaluated at the time of transport to slaughter. Eighty steers (BW = 668 ± 36 kg) representing 10 lots of similar breed, frame size, and degree of finish were blocked by lot, stratified by weight, and randomly assigned to 1 of 2 handling intensities (HI) over a 1,600 m dirt alley course: 1) low-stress handling (LSH) or 2) high-stress handling (HSH). For the LSH treatment, 4 penmates were kept at a walk with the use of a lead rider...
May 2017: Journal of Animal Science
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