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https://www.readbyqxmd.com/read/28086772/scutellaria-barbata-d-don-extract-inhibits-the-tumor-growth-through-down-regulating-of-treg-cells-and-manipulating-th1-th17-immune-response-in-hepatoma-h22-bearing-mice
#1
Xuefeng Kan, Wanli Zhang, Ruxu You, Yanfeng Niu, Jianrong Guo, Jun Xue
BACKGROUND: Previous studies showed Scutellaria barbata D. Don extract (SBE) is a potent inhibitor in hepatoma and could improve immune function of hepatoma H22-bearing mice. However, the immunomodulatory function of SBE on the tumor growth of hepatoma remains unclear. This study aimed to investigate the anti-tumor effects of SBE on hepatoma H22-bearing mice and explore the underlying immunomodulatory function. METHODS: The hepatoma H22-bearing mice were treated by SBE for 30 days...
January 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28008628/analysis-of-four-pcr-snapshot-multiplex-assays-analyzing-52-snpforid-markers
#2
Goodwin Will, Alimat Sharizah
The SNPforID consortium identified a panel of 52 SNPs forensic analysis that has been used by several laboratories worldwide. The original analysis of the 52 SNPs was based on a single multiplex reaction followed by two single-base-extension (SBE) reactions each of which was analyzed using capillary electrophoresis. The SBE assays were designed for high throughput genetic analyzers and were difficult to use on the single capillary ABI PRISM 310 Genetic Analyzer and the latest generation 3500 Genetic Analyzer, as sensitivity on the 310 was low and separation of products on the 3500 with POP-7™ was poor...
December 23, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27994606/lineage-specific-evolutionary-histories-and-regulation-of-major-starch-metabolism-genes-during-banana-ripening
#3
Cyril Jourda, Céline Cardi, Olivier Gibert, Andrès Giraldo Toro, Julien Ricci, Didier Mbéguié-A-Mbéguié, Nabila Yahiaoui
Starch is the most widespread and abundant storage carbohydrate in plants. It is also a major feature of cultivated bananas as it accumulates to large amounts during banana fruit development before almost complete conversion to soluble sugars during ripening. Little is known about the structure of major gene families involved in banana starch metabolism and their evolution compared to other species. To identify genes involved in banana starch metabolism and investigate their evolutionary history, we analyzed six gene families playing a crucial role in plant starch biosynthesis and degradation: the ADP-glucose pyrophosphorylases (AGPases), starch synthases (SS), starch branching enzymes (SBE), debranching enzymes (DBE), α-amylases (AMY) and β-amylases (BAM)...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27991590/polarization-independent-split-bull-s-eye-antennas-for-infrared-nano-photodetectors
#4
Meng Yang, Fang-Fang Ren, Lin Pu, Long Xiao, Yun Sheng, Junzhuan Wang, Hai Lu, Youdou Zheng, Yi Shi
Split bull's eye (SBE) antennas exhibit much larger extraordinary optical transmission and strong polarization dependence rather than bull's eye (BE) antennas in the infrared range due to the introduced sub-wavelength slit. Here, we demonstrate a dual-split bull's eye (DSBE) antenna, which consists of two sub-wavelength slits crossing through the center of the BE antenna with an intersection angle θ. The polarization dependence in transmission can be flexibly tailored by adjusting the intersection angle, following a cos(2) (Φ + θ/2) angular dependence on polarization angle Φ...
December 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27973358/removal-of-methylene-blue-dye-from-water-by-a-spent-bleaching-earth-biosorbent
#5
Ali Belhaine, Mouffok Redouane Ghezzar, Fatiha Abdelmalek, Kamel Tayebi, Abdelhamid Ghomari, Ahmed Addou
This work is about the valorization of spent bleaching earth (SBE) at low cost from the AFIA oil refinery in Algeria's western region. The biowaste has been burned to eliminate the oil traces, then it has been valorized in two ways: (i) chemically, using phosphoric acid to obtain SBEC and (ii) thermally at 750 °C to create SBEH. The obtained materials were used for the first time as biosorbents in the wastewater treatment field. The three biomaterials show very interesting adsorption properties, with specific surfaces of 194...
December 2016: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/27941610/subcritical-butane-extraction-of-wheat-germ-oil-and-its-deacidification-by-molecular-distillation
#6
Jinwei Li, Dewei Sun, Lige Qian, Yuanfa Liu
Extraction and deacidification are important stages for wheat germ oil (WGO) production. Crude WGO was extracted using subcritical butane extraction (SBE) and compared with traditional solvent extraction (SE) and supercritical carbon dioxide extraction (SCE) based on the yield, chemical index and fatty acid profile. Furthermore, the effects of the molecular distillation temperature on the quality of WGO were also investigated in this study. Results indicated that WGO extracted by SBE has a higher yield of 9...
December 7, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27924122/a-tomato-plastidic-atp-adp-transporter-gene-slaatp-increases-starch-content-in-transgenic-arabidopsis
#7
Feibing Wang, Yuxiu Ye, Yuan Niu, Faxiang Wan, Bo Qi, Xinhong Chen, Qing Zhou, Boqing Chen
A plastidic ATP/ADP transporter (AATP) is responsible for importing ATP from the cytosol into plastids. Increasing the ATP supply is a potential way to facilitate anabolic synthesis in heterotrophic plastids of plants. In this work, a gene encoding the AATP protein, named SlAATP, was successfully isolated from tomato. Expression of SlAATP was induced by exogenous sucrose treatment in tomato. The coding region of SlAATP was cloned into a binary vector under the control of 35S promoter and then transformed into Arabidopsis to obtain transgenic plants...
October 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27916704/design-and-development-of-dry-powder-sulfobutylether-%C3%AE-cyclodextrin-complex-for-pulmonary-delivery-of-fisetin
#8
Noratiqah Mohtar, Kevin M G Taylor, Khalid Sheikh, Satyanarayana Somavarapu
This study has investigated complexation of fisetin, a natural flavonoid, with three types of cyclodextrins to improve its solubility. Sulfobutylether-β-cyclodextrin (SBE-β-CD) showed the highest complexation efficiency while maintaining the in vitro antioxidant activity of fisetin. Addition of 20%v/v ethanol in water improved the amount of solubilized fisetin in the complex 5.9-fold compared to the system containing water alone. Spray drying of fisetin-SBE-β-CD complex solution in the presence of ethanol produced a dry powder with improved aerosolization properties when delivered from a dry powder inhaler, indicated by a 2-fold increase in the fine particle fraction (FPF) compared to the powder produced from the complex solution containing water alone...
December 1, 2016: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/27916068/-the-correlation-between-cerebral-mri-characteristics-of-posterior-reversible-encephalopathy-syndrome-and-serum-levels-of-plgf-seng-in-patients-with-pre-eclampsia
#9
K M Jiang, X Zhong, Y Tan, G C Liu, H Mai, S X Wu
Objective: To investigate the MRI characteristics of posterior reversible encephalopathy syndrome (PRES) in patients with pre-eclampsia(PE) and its correlation with serum placental growth factor (PlGF), soluble endoglin (sEng) and sEng/PlGF ratio. Methods: 34 patients with PE who were admitted to Guangdong Women and Children Hospital from September 2013 to March 2014 were studied retrospectively.13 cases had PRES and 21 cases had normal cerebral MRI. All patients underwent conventional MRI and DWI scanning...
November 25, 2016: Zhonghua Fu Chan Ke za Zhi
https://www.readbyqxmd.com/read/27896020/identification-of-phenolic-secondary-metabolites-from-schotia-brachypetala-sond-fabaceae-and-demonstration-of-their-antioxidant-activities-in-caenorhabditis-elegans
#10
Mansour Sobeh, Esraa ElHawary, Herbenya Peixoto, Rola M Labib, Heba Handoussa, Noha Swilam, Ahmed H El-Khatib, Farukh Sharapov, Tamer Mohamed, Sonja Krstin, Michael W Linscheid, Abdel Nasser Singab, Michael Wink, Nahla Ayoub
BACKGROUND: Schotia brachypetala Sond. (Fabaceae) is an endemic tree of Southern Africa whose phytochemistry and pharmacology were slightly studied. The present work aimed at profiling the major phenolics compounds present in the hydro-alcohol extract from S. brachypetala leaves (SBE) using LC/HRESI/MS/MS and NMR and prove their antioxidant capabilities using novel methods. METHODS: In vitro assays; DPPH, TEAC persulfate decolorizing kinetic and FRAP assays, and in vivo assays: Caenorhabditis elegans strains maintenance, Intracellular ROS in C...
2016: PeerJ
https://www.readbyqxmd.com/read/27863739/the-influence-of-testing-angle-on-the-biomechanical-properties-of-the-rat-supraspinatus-tendon
#11
Michael D Newton, Abigail A Davidson, Ryan Pomajzl, Joseph Seta, Michael D Kurdziel, Tristan Maerz
Rotator cuff tears are a common shoulder pathology. The rat supraspinatus tendon model is commonly employed for preclinical assessment of rotator cuff pathology or regeneration. However, there is a lack of a standardized biomechanical testing protocol; previous studies have tested the tendon at abduction angles ranging from -15° to 90°. This study aimed to assess the effect of abduction/testing angle on the biomechanical properties of the rat supraspinatus tendon. Fourty-eight shoulders (n=12/group) from healthy Sprague-Dawley rats were randomized to 4 testing angle groups: 0° (corresponding to 90° abduction), 30°, 60°, and 90° (0° abduction)...
December 8, 2016: Journal of Biomechanics
https://www.readbyqxmd.com/read/27857520/secondary-elements-of-blood-ph-variation-can-influence-the-effort-effectiveness-based-on-adaptive-changes-within-a-group-of-elite-athletes
#12
Ştefan Adrian Martin, Valeriu Tomescu, Septimiu Voidăzan
AIM: pH is the direct indicator of the body reaction following the activities performed. Establishing precise correlations between pH and blood biochemical parameters might support the balancing of values during periods of marked physical activity. METHOD: We conducted a case study in a group of elite rowers. Twelve athletes were included in the study. Monitoring was carried out by collecting biological samples several times a day: in the morning, 80 minutes pre-workout, 12 hours after the last physical effort performed, at two different times, 10 days apart...
2016: Clujul Medical (1957)
https://www.readbyqxmd.com/read/27855897/insights-into-molecular-structure-and-digestion-rate-of-oat-starch
#13
Jinchuan Xu, Qirong Kuang, Kai Wang, Sumei Zhou, Shuo Wang, Xingxun Liu, Shujun Wang
The in vitro digestibility of oat starch and its relationship with starch molecular structure was investigated. The in vitro digestion results showed that the first-order kinetic constant (k) of oat starches (OS-1 and OS-2) was lower than that of rice starch. The size of amylose chains, amylose content and degree of branching (DB) of amylopectin in oat starch were significantly higher than the corresponding parameters in rice starch. The larger molecular size of oat starch may account for its lower digestion rate...
April 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27784353/-effects-of-scutellaria-barbata-on-vegf-expression-in-k562-cells
#14
Rui Shi, Su-Qing Guo, Shan Liu, Zhi-Shang Li, Jun-Jun Li
OBJECTIVE: To investigate the anti-angiogenesis effect of Scutellaria barbata extract(SBE) on chronic myeloid leukemia(CML) K562 cell line in vitro. METHODS: The proliferating activity after treating K562 cells with 0.5,1.0,2.0 and 4.0 g/ml SB for 24, 36, 48 hours were assessed by MTT assay. The level of vascular endothelial growth factor(VEGF) in the culture supematant of K562 cells was determined by ELISA; and the expression of VEGF mRNA was detected by RT-PCR...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27783470/a-novel-method-of-surface-modification-by-electrochemical-deoxidation-effect-on-surface-characteristics-and-initial-bioactivity-of-zirconia
#15
Juan Liu, Guang Hong, Yu-Han Wu, Kosei Endo, Jian-Min Han, Hiroyuki Kumamoto, Takeshi Wada, Hidemi Kato, Ping Gao, Keiichi Sasaki
The aim of this study was to investigate and compare the surface characteristics and initial bioactivity of ceria-stabilized zirconia/alumina nanocomposite (NANOZR) with those of yttria-stabilized zirconia (3Y-TZP) and pure titanium (CpTi) following the use of three surface modification methods; polishing, sandblasting/acid-etching (SB-E) and electrochemical deoxidation (ECD). Physical properties including surface morphology, chemical composition, X-ray diffraction, surface wettability, surface roughness, and hardness were measured...
October 26, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27760436/short-type-single-balloon-enteroscope-assisted-ercp-in-postsurgical-altered-anatomy-potential-factors-affecting-procedural-failure
#16
Kei Yane, Akio Katanuma, Hiroyuki Maguchi, Kuniyuki Takahashi, Toshifumi Kin, Satoshi Ikarashi, Itsuki Sano, Hajime Yamazaki, Koh Kitagawa, Kensuke Yokoyama, Hideaki Koga, Kazumasa Nagai, Masanori Nojima
Background and study aims Short-type single-balloon enteroscope (short SBE)-assisted endoscopic retrograde cholangiopancreatography (ERCP) is a promising alternative treatment in postsurgical altered anatomy. However, it is technically demanding, and factors affecting its technical difficulty have not yet been clarified. This study aimed to examine the procedural success rate of short SBE-assisted ERCP and the potential factors affecting procedural failure. Patients and methods A total of 117 consecutive patients (203 procedures) with surgically altered anatomy underwent ERCP using prototype short SBEs...
January 2017: Endoscopy
https://www.readbyqxmd.com/read/27757923/preparation-and-characterization-of-the-sulfobutylether-%C3%AE-cyclodextrin-inclusion-complex-of-amiodarone-hydrochloride-with-enhanced-oral-bioavailability-in-fasted-state
#17
Dandan Wang, Guoguang Chen, Lili Ren
Amiodarone hydrochloride (AMD) is used in the treatment of a wide range of cardiac tachyarrhythmias, including both ventricular fibrillation (VF) and hemodynamically unstable ventricular tachycardia (VT). The objectives of this study were to improve the solubility and bioavailability in fasted state and to reduce the food effect of AMD by producing its inclusion complex with sulfobutylether-β-cyclodextrin (SBE-β-CD). The complex was prepared through a saturated water solution combined with the freeze-drying method and then characterized by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and differential scanning calorimetry...
October 18, 2016: AAPS PharmSciTech
https://www.readbyqxmd.com/read/27744574/tgf%C3%AE-contributes-to-the-anti-inflammatory-effects-of-tauroursodeoxycholic-acid-on-an-animal-model-of-acute-neuroinflammation
#18
Natalia Yanguas-Casás, M Asunción Barreda-Manso, Sandra Pérez-Rial, Manuel Nieto-Sampedro, Lorenzo Romero-Ramírez
The bile acid conjugate tauroursodeoxycholic acid (TUDCA) is a neuroprotective agent in various animal models of neuropathologies. We have previously shown the anti-inflammatory properties of TUDCA in an animal model of acute neuroinflammation. Here, we present a new anti-inflammatory mechanism of TUDCA through the regulation of transforming growth factor β (TGFβ) pathway. The bacterial lipopolysaccharide (LPS) was injected intravenously (iv) on TGFβ reporter mice (Smad-binding element (SBE)/Tk-Luc) to study in their brains the real-time activation profile of the TGFβ pathway in a non-invasive way...
October 15, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27716051/development-of-ems-induced-mutation-population-for-amylose-and-resistant-starch-variation-in-bread-wheat-triticum-aestivum-and-identification-of-candidate-genes-responsible-for-amylose-variation
#19
Ankita Mishra, Anuradha Singh, Monica Sharma, Pankaj Kumar, Joy Roy
BACKGROUND: Starch is a major part of cereal grain. It comprises two glucose polymer fractions, amylose (AM) and amylopectin (AP), that make up about 25 and 75 % of total starch, respectively. The ratio of the two affects processing quality and digestibility of starch-based food products. Digestibility determines nutritional quality, as high amylose starch is considered a resistant or healthy starch (RS type 2) and is highly preferred for preventive measures against obesity and related health conditions...
October 6, 2016: BMC Plant Biology
https://www.readbyqxmd.com/read/27609711/microvesicles-from-brain-extract-treated-mesenchymal-stem-cells-improve-neurological-functions-in-a-rat-model-of-ischemic-stroke
#20
Ji Yong Lee, Eiru Kim, Seong-Mi Choi, Dong-Wook Kim, Kwang Pyo Kim, Insuk Lee, Han-Soo Kim
Transplantation of mesenchymal stem cells (MSCs) was reported to improve functional outcomes in a rat model of ischemic stroke, and subsequent studies suggest that MSC-derived microvesicles (MVs) can replace the beneficial effects of MSCs. Here, we evaluated three different MSC-derived MVs, including MVs from untreated MSCs (MSC-MVs), MVs from MSCs treated with normal rat brain extract (NBE-MSC-MVs), and MVs from MSCs treated with stroke-injured rat brain extract (SBE-MSC-MVs), and tested their effects on ischemic brain injury induced by permanent middle cerebral artery occlusion (pMCAO) in rats...
2016: Scientific Reports
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