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https://www.readbyqxmd.com/read/28326091/generation-of-high-amylose-rice-through-crispr-cas9-mediated-targeted-mutagenesis-of-starch-branching-enzymes
#1
Yongwei Sun, Guiai Jiao, Zupei Liu, Xin Zhang, Jingying Li, Xiuping Guo, Wenming Du, Jinlu Du, Frédéric Francis, Yunde Zhao, Lanqin Xia
Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. However, it remains a challenge to control starch branching in commercial lines. Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in SBEI and SBEIIb in rice. The frequencies of obtained homozygous or bi-allelic mutant lines with indels in SBEI and SBEIIb in T0 generation were from 26...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28299353/safety-and-efficacy-of-single-balloon-enteroscopy-in-management-of-gastrointestinal-bleeding-in-patients-with-a-left-ventricular-assist-device
#2
Abhinav Koul, Donald M Pham, Arjun Nanda, Kevin E Woods, Steven D Keilin
Background and study aims Left ventricular assist devices (LVADs) are currently the standard of care in treatment of patients with end-stage heart failure waiting for heart transplant as well as destination therapy for non-transplant candidates. However, patients with LVADs are at increased risk of gastrointestinal bleeding due to the device's unique effects on hemodynamics. A major source of gastrointestinal bleeding in these patients are gastrointestinal angioectasias located within the small bowel that can only be reached with deep enteroscopy...
March 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28299150/awareness-and-practice-of-self-breast-examination-among-female-nurses-at-the-federal-teaching-hospital-ido-ekiti-nigeria
#3
Olusegun Emmanuel Gabriel, Oluwaserimi Adewumi Ajetunmobi, Olabode Muftau Shabi, Olaide Toyin Elegbede, Raymond Akujuobi Okere, Olusegun Adesola Busari, Ayokunle Samuel Dada
Breast cancer is the most common female malignancy linked with high levels of morbidity and mortality in developing countries due to delayed diagnosis. This research assessed the knowledge of signs and risk factors of breast cancer and practice of self breast examination (SBE) among female nurses in a rural tertiary Hospital. Eighty-five nurses ages 20 to 60 years were sampled by simple randomization over a period of eight weeks through a self-administered semi-structured questionnaire. The analysis was done using statistical package for social science version 17...
August 17, 2016: Journal of Public Health in Africa
https://www.readbyqxmd.com/read/28256567/a-wogonin-rich-fraction-of-scutellaria-baicalensis-root-extract-exerts-chondroprotective-effects-by-suppressing-il-1%C3%AE-induced-activation-of-ap-1-in-human-oa-chondrocytes
#4
Nazir M Khan, Abdul Haseeb, Mohammad Y Ansari, Tariq M Haqqi
Osteoarthritis (OA) is a common joint disorder with varying degrees of inflammation and sustained oxidative stress. The root extract of Scutellaria baicalensis (SBE) has been used for the treatment of inflammatory and other diseases. Here, we performed activity-guided HPLC-fractionation of SBE, identified the active ingredient(s) and investigated its chondroprotective potential. We found that the Wogonin containing fraction-4 (F4) was the most potent fraction based on its ability to inhibit ROS production and the suppression of catabolic markers including IL-6, COX-2, iNOS, MMP-3, MMP-9, MMP-13 and ADAMTS-4 in IL-1β-treated OA chondrocytes...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28248993/role-of-computed-tomography-angiography-on-the-management-of-overt-obscure-gastrointestinal-bleeding
#5
Chao-Ming Tseng, I-Chang Lin, Chi-Yang Chang, Hsiu-Po Wang, Chih-Cheng Chen, Lein-Ray Mo, Jaw-Town Lin, Chi-Ming Tai
BACKGROUND AND AIM: The role of computed tomography angiography (CTA) on the management of acute overt obscure gastrointestinal bleeding (OGIB) remains unclear. We designed a study to evaluate the impact of CTA before enteroscopy for acute overt OGIB. METHODS: All patients undergoing CTA followed by enteroscopy for acute overt OGIB were enrolled in this retrospective study. Clinical characteristics and diagnosis were compared between patients with positive and negative CTA findings...
2017: PloS One
https://www.readbyqxmd.com/read/28216462/a-study-of-the-aggregation-of-cyclodextrins-determination-of-the-critical-aggregation-concentration-size-of-aggregates-and-thermodynamics-using-isodesmic-and-k2-k-models
#6
Thao Thi Do, Rob Van Hooghten, Guy Van den Mooter
The aggregation of three different cyclodextrins (CDs): 2-hydroxypropyl-β-cyclodextrin (HP-β-CD), 2-hydroxypropyl-γ-cyclodextrin (HP-γ-CD) and sulfobutylether-β-cyclodextrin (SBE-β-CD) was studied. The critical aggregation concentration (cac) of these three CDs is quite similar and is situated at ca. 2% (m/v). There was only a small difference in the cac values determined by DLS and (1)H NMR. DLS measurements revealed that CDs in solution have three size populations wherein one of them is that of a single CD molecule...
February 17, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28210706/single-balloon-enteroscopy-efficacy-and-degree-of-concordance-with-noninvasive-evaluation-of-small-bowel
#7
Margarida Marques, João Antunes, Rosa Coelho, Hélder Cardoso, Filipe Vilas Boas, Armando Ribeiro, Guilherme Macedo
Background and study aims Clinical impact of single-balloon enteroscopy (SBE) is fairly known, as well as its diagnostic yield comparing with other small bowel gastrointestinal investigations. This study represents a contribution to better understand it and is designed to evaluate SBE efficacy and degree of concordance with previous evaluation of small bowel. Patients and methods This is a single-center retrospective study of patients that underwent SBE with suspected small bowel disease based on non-invasive imaging...
February 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28155073/nimodipine-ophthalmic-formulations-for-management-of-glaucoma
#8
Doaa Nabih Maria, Abd-Elgawad Helmy Abd-Elgawad, Osama Abd-Elazeem Soliman, Marwa Salah El-Dahan, Monica M Jablonski
PURPOSE: Preparation and evaluation of topical ophthalmic formulations containing nimodipine-CD complexes prepared using HP-β-CD, SBE-β-CD and M-β-CD for the management of glaucoma. METHODS: Nimodipine-CD complexes were prepared using a freeze-drying method. Two different molar ratios (NMD:CD) were used for each cyclodextrin. The inclusion complexes were characterized using DSC, FTIR, yield (%), drug content and in vitro release characteristics. NMD-CD complexes incorporated into chitosan eye drops and a temperature-triggered in situ gelling system were evaluated for their pH, viscosity and in vitro release characteristics...
April 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28119267/forensic-snp-genotyping-with-snapshot-technical-considerations-for-the-development-and-optimization-of-multiplexed-snp-assays
#9
REVIEW
M Fondevila, C Børsting, C Phillips, M de la Puente, Euroforen-NoE Consortium, A Carracedo, N Morling, M V Lareu
This review explores the key factors that influence the optimization, routine use, and profile interpretation of the SNaPshot single-base extension (SBE) system applied to forensic single-nucleotide polymorphism (SNP) genotyping. Despite being a mainly complimentary DNA genotyping technique to routine STR profiling, use of SNaPshot is an important part of the development of SNP sets for a wide range of forensic applications with these markers, from genotyping highly degraded DNA with very short amplicons to the introduction of SNPs to ascertain the ancestry and physical characteristics of an unidentified contact trace donor...
January 2017: Forensic Science Review
https://www.readbyqxmd.com/read/28107548/mri-based-prostate-cancer-detection-with-high-level-representation-and-hierarchical-classification
#10
Yulian Zhu, Li Wang, Mingxia Liu, Chunjun Qian, Ambereen Yousuf, Aytekin Oto, Dinggang Shen
PURPOSE: Extracting the high-level feature representation by using deep neural networks for detection of prostate cancer, and then based on high-level feature representation for constructing hierarchical classification to refine the detection results. METHODS: High-level feature representation is first learned by a deep learning network, where multi-parametric MR images are used as the input data. Then, based on the learned high-level features, a hierarchical classification method is developed, where multiple random forest classifiers are iteratively constructed to refine the detection results of prostate cancer...
January 20, 2017: Medical Physics
https://www.readbyqxmd.com/read/28086772/scutellaria-barbata-d-don-extract-inhibits-the-tumor-growth-through-down-regulating-of-treg-cells-and-manipulating-th1-th17-immune-response-in-hepatoma-h22-bearing-mice
#11
Xuefeng Kan, Wanli Zhang, Ruxu You, Yanfeng Niu, Jianrong Guo, Jun Xue
BACKGROUND: Previous studies showed Scutellaria barbata D. Don extract (SBE) is a potent inhibitor in hepatoma and could improve immune function of hepatoma H22-bearing mice. However, the immunomodulatory function of SBE on the tumor growth of hepatoma remains unclear. This study aimed to investigate the anti-tumor effects of SBE on hepatoma H22-bearing mice and explore the underlying immunomodulatory function. METHODS: The hepatoma H22-bearing mice were treated by SBE for 30 days...
January 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28008628/analysis-of-four-pcr-snapshot-multiplex-assays-analyzing-52-snpforid-markers
#12
Goodwin Will, Alimat Sharizah
The SNPforID consortium identified a panel of 52 SNPs forensic analysis that has been used by several laboratories worldwide. The original analysis of the 52 SNPs was based on a single multiplex reaction followed by two single-base-extension (SBE) reactions each of which was analyzed using capillary electrophoresis. The SBE assays were designed for high throughput genetic analyzers and were difficult to use on the single capillary ABI PRISM 310 Genetic Analyzer and the latest generation 3500 Genetic Analyzer, as sensitivity on the 310 was low and separation of products on the 3500 with POP-7™ was poor...
December 23, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27994606/lineage-specific-evolutionary-histories-and-regulation-of-major-starch-metabolism-genes-during-banana-ripening
#13
Cyril Jourda, Céline Cardi, Olivier Gibert, Andrès Giraldo Toro, Julien Ricci, Didier Mbéguié-A-Mbéguié, Nabila Yahiaoui
Starch is the most widespread and abundant storage carbohydrate in plants. It is also a major feature of cultivated bananas as it accumulates to large amounts during banana fruit development before almost complete conversion to soluble sugars during ripening. Little is known about the structure of major gene families involved in banana starch metabolism and their evolution compared to other species. To identify genes involved in banana starch metabolism and investigate their evolutionary history, we analyzed six gene families playing a crucial role in plant starch biosynthesis and degradation: the ADP-glucose pyrophosphorylases (AGPases), starch synthases (SS), starch branching enzymes (SBE), debranching enzymes (DBE), α-amylases (AMY) and β-amylases (BAM)...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27991590/polarization-independent-split-bull-s-eye-antennas-for-infrared-nano-photodetectors
#14
Meng Yang, Fang-Fang Ren, Lin Pu, Long Xiao, Yun Sheng, Junzhuan Wang, Hai Lu, Youdou Zheng, Yi Shi
Split bull's eye (SBE) antennas exhibit much larger extraordinary optical transmission and strong polarization dependence rather than bull's eye (BE) antennas in the infrared range due to the introduced sub-wavelength slit. Here, we demonstrate a dual-split bull's eye (DSBE) antenna, which consists of two sub-wavelength slits crossing through the center of the BE antenna with an intersection angle θ. The polarization dependence in transmission can be flexibly tailored by adjusting the intersection angle, following a cos(2) (Φ + θ/2) angular dependence on polarization angle Φ...
December 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27973358/removal-of-methylene-blue-dye-from-water-by-a-spent-bleaching-earth-biosorbent
#15
Ali Belhaine, Mouffok Redouane Ghezzar, Fatiha Abdelmalek, Kamel Tayebi, Abdelhamid Ghomari, Ahmed Addou
This work is about the valorization of spent bleaching earth (SBE) at low cost from the AFIA oil refinery in Algeria's western region. The biowaste has been burned to eliminate the oil traces, then it has been valorized in two ways: (i) chemically, using phosphoric acid to obtain SBEC and (ii) thermally at 750 °C to create SBEH. The obtained materials were used for the first time as biosorbents in the wastewater treatment field. The three biomaterials show very interesting adsorption properties, with specific surfaces of 194...
December 2016: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/27941610/subcritical-butane-extraction-of-wheat-germ-oil-and-its-deacidification-by-molecular-distillation
#16
Jinwei Li, Dewei Sun, Lige Qian, Yuanfa Liu
Extraction and deacidification are important stages for wheat germ oil (WGO) production. Crude WGO was extracted using subcritical butane extraction (SBE) and compared with traditional solvent extraction (SE) and supercritical carbon dioxide extraction (SCE) based on the yield, chemical index and fatty acid profile. Furthermore, the effects of the molecular distillation temperature on the quality of WGO were also investigated in this study. Results indicated that WGO extracted by SBE has a higher yield of 9...
December 7, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27924122/a-tomato-plastidic-atp-adp-transporter-gene-slaatp-increases-starch-content-in-transgenic-arabidopsis
#17
Feibing Wang, Yuxiu Ye, Yuan Niu, Faxiang Wan, Bo Qi, Xinhong Chen, Qing Zhou, Boqing Chen
A plastidic ATP/ADP transporter (AATP) is responsible for importing ATP from the cytosol into plastids. Increasing the ATP supply is a potential way to facilitate anabolic synthesis in heterotrophic plastids of plants. In this work, a gene encoding the AATP protein, named SlAATP, was successfully isolated from tomato. Expression of SlAATP was induced by exogenous sucrose treatment in tomato. The coding region of SlAATP was cloned into a binary vector under the control of 35S promoter and then transformed into Arabidopsis to obtain transgenic plants...
October 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27916704/design-and-development-of-dry-powder-sulfobutylether-%C3%AE-cyclodextrin-complex-for-pulmonary-delivery-of-fisetin
#18
Noratiqah Mohtar, Kevin M G Taylor, Khalid Sheikh, Satyanarayana Somavarapu
This study has investigated complexation of fisetin, a natural flavonoid, with three types of cyclodextrins to improve its solubility. Sulfobutylether-β-cyclodextrin (SBE-β-CD) showed the highest complexation efficiency while maintaining the in vitro antioxidant activity of fisetin. Addition of 20%v/v ethanol in water improved the amount of solubilized fisetin in the complex 5.9-fold compared to the system containing water alone. Spray drying of fisetin-SBE-β-CD complex solution in the presence of ethanol produced a dry powder with improved aerosolization properties when delivered from a dry powder inhaler, indicated by a 2-fold increase in the fine particle fraction (FPF) compared to the powder produced from the complex solution containing water alone...
April 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/27916068/-the-correlation-between-cerebral-mri-characteristics-of-posterior-reversible-encephalopathy-syndrome-and-serum-levels-of-plgf-seng-in-patients-with-pre-eclampsia
#19
K M Jiang, X Zhong, Y Tan, G C Liu, H Mai, S X Wu
Objective: To investigate the MRI characteristics of posterior reversible encephalopathy syndrome (PRES) in patients with pre-eclampsia(PE) and its correlation with serum placental growth factor (PlGF), soluble endoglin (sEng) and sEng/PlGF ratio. Methods: 34 patients with PE who were admitted to Guangdong Women and Children Hospital from September 2013 to March 2014 were studied retrospectively.13 cases had PRES and 21 cases had normal cerebral MRI. All patients underwent conventional MRI and DWI scanning...
November 25, 2016: Zhonghua Fu Chan Ke za Zhi
https://www.readbyqxmd.com/read/27896020/identification-of-phenolic-secondary-metabolites-from-schotia-brachypetala-sond-fabaceae-and-demonstration-of-their-antioxidant-activities-in-caenorhabditis-elegans
#20
Mansour Sobeh, Esraa ElHawary, Herbenya Peixoto, Rola M Labib, Heba Handoussa, Noha Swilam, Ahmed H El-Khatib, Farukh Sharapov, Tamer Mohamed, Sonja Krstin, Michael W Linscheid, Abdel Nasser Singab, Michael Wink, Nahla Ayoub
BACKGROUND: Schotia brachypetala Sond. (Fabaceae) is an endemic tree of Southern Africa whose phytochemistry and pharmacology were slightly studied. The present work aimed at profiling the major phenolics compounds present in the hydro-alcohol extract from S. brachypetala leaves (SBE) using LC/HRESI/MS/MS and NMR and prove their antioxidant capabilities using novel methods. METHODS: In vitro assays; DPPH, TEAC persulfate decolorizing kinetic and FRAP assays, and in vivo assays: Caenorhabditis elegans strains maintenance, Intracellular ROS in C...
2016: PeerJ
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