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https://www.readbyqxmd.com/read/29133372/gradually-decreasing-starch-branching-enzyme-expression-is-responsible-for-the-formation-of-heterogeneous-starch-granules
#1
Juan Wang, Pan Hu, Lingshang Lin, Zichun Chen, Qiao Quan Liu, Cunxu Wei
Rice endosperm is mainly occupied by homogeneous polygonal starch from inside to outside. However, morphologically different (heterogeneous) starches have been identified in rice mutants. How these heterogeneous starches form remains unknown. A high-amylose rice line (TRS) generated through the antisense inhibition of starch branching synthase (SBE) I and IIb contains four heterogeneous starches: polygonal, aggregate, elongated, and hollow starch; these starches are regionally distributed in the endosperm from inside to outside...
November 13, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29097649/characteristics-of-nonsteroidal-anti-inflammatory-drugs-nsaids-induced-small-bowel-injury-identified-by-single-balloon-endoscopy-or-capsule-endoscopy
#2
Ning Xu, Zhenhai Yu, Xiaoling Cao, Zhihua Wang, Ming Yan
BACKGROUND The special features of nonsteroidal anti-inflammatory drugs (NSAIDs) enteropathy were partially clarified by single-balloon endoscopy(SBE). We aimed to investigate the characteristics of NSAIDs injuries that were differ from other ulcer diseases and efficacy of SBE compared with capsule endoscopy(CE). MATERIAL AND METHODS 1,644 symptomatic patients (221 patients taking NSAIDs) hospitalized between January 2006 and March 2016 were recruited and underwent SBE and/or CE. RESULTS NSAIDs damages were identified in 110 patients (49...
November 3, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29094956/food-addiction-prevalence-and-concurrent-validity-in-african-american-adolescents-with-obesity
#3
Erica M Schulte, Angela J Jacques-Tiura, Ashley N Gearhardt, Sylvie Naar
Food addiction, measured by the Yale Food Addiction Scale (YFAS), has been associated with obesity, eating-related problems (e.g., bingeing), and problematic consumption of highly processed foods. Studies on this topic have primarily examined adult samples with an overrepresentation of White individuals, and little is known about addictive-like eating in adolescents, particularly African American adolescents who exhibit high rates of obesity and eating pathology. The current study examined the prevalence of food addiction and its convergent validity with percent overweight, eating-related problems, and self-reported dietary intake in a sample of 181 African American adolescents with obesity...
November 2, 2017: Psychology of Addictive Behaviors: Journal of the Society of Psychologists in Addictive Behaviors
https://www.readbyqxmd.com/read/29053634/petroleum-hydrocarbon-profiles-of-water-and-sediment-of-algoa-bay-eastern-cape-south-africa
#4
Abiodun O Adeniji, Omobola O Okoh, Anthony I Okoh
Petroleum hydrocarbon profiles of water and sediment samples of Algoa Bay in the Eastern Cape Province of South Africa were assessed using standard analytical procedures. Water (from surface and bottom levels) and sediment samples were collected from five locations in the bay from February to June 2016. Extraction of the petroleum hydrocarbons from the water and sediment samples collected was achieved using liquid-liquid and Soxhlet extraction techniques, respectively, followed by column clean up. Target compounds were analytically determined with gas chromatography-flame ionization detector (GC-FID) and quantified by integrating the areas of both the resolved and unresolved components...
October 20, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29050223/mir-23a-promotes-tgf-%C3%AE-1-induced-emt-and-tumor-metastasis-in-breast-cancer-cells-by-directly-targeting-cdh1-and-activating-wnt-%C3%AE-catenin-signaling
#5
Fei Ma, Wenjie Li, Chunxiao Liu, Wei Li, Haining Yu, Bo Lei, Yanlv Ren, Zhigao Li, Da Pang, Cheng Qian
TGF-β1-induced epithelial-mesenchymal transition (EMT) has been proved to be associated with metastasis of breast cancer cells. We attempted to detect a novel mechanism that microRNAs mediated the TGF-β1-induced EMT in the process of breast cancer metastasis. Here we reported that the expression of miR-23a was higher in breast cancer cells with high metastasis ability and patients with lymph node metastasis and the treatment of TGF-β1 significantly upregulated the expression of miR-23a in breast cancer cells...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29042336/physicochemical-properties-of-inclusion-complexes-of-highly-soluble-%C3%AE-cyclodextrins-with-highly-hydrophobic-testosterone-propionate
#6
Christian Celia, Angela Scala, Rosanna Stancanelli, Emanuela Surdo, Donatella Paolino, Alessandro Grattoni, Nicola Micale, Vincenza Crupi, Domenico Majolino, Massimo Fresta, Silvana Tommasini, Valentina Venuti, Cinzia Anna Ventura
Hydroxypropyl-β-cyclodextrin (HP-β-CyD) and sulfobutyl ether-β-cyclodextrin (SBE-β-CyD) were used to generate hydrophilic complexes of the poorly water-soluble drug testosterone propionate (TP). The inclusion complexes were obtained by freeze-drying, and then analyzed at both liquid and solid states. Phase solubility studies, performed according to the type-AL solubility diagrams of TP in presence of both CyDs, suggested the formation of water-soluble complexes at 1:1 molar ratio. These results were confirmed by continuous variation method (Job's plot)...
October 16, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29036352/environmental-plasticity-in-the-intersexual-correlation-and-sex-bias-of-gene-expression
#7
Changde Cheng, Mark Kirkpatrick, Melissa Wilson Sayres
Intersexual genetic correlations are expected to constrain the evolution of sexual dimorphic traits, including the degree of sex-biased gene expression. Consistent with that expectation, studies in fruit flies and birds have reported that genes whose expression has a strong intersexual genetic correlation (rMF) show a lower level of sex-biased expression (SBE). However, it is known that both rMF and SBE can be affected by the environment. It is therefore unclear whether there is a consistent relationship between these 2 quantities across multiple environments...
October 30, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/28979274/biochemical-defense-response-characterizing-the-plasticity-of-source-and-sink-in-spring-wheat-under-terminal-heat-stress
#8
Ranjeet R Kumar, Suneha Goswami, Mohammed Shamim, Upama Mishra, Monika Jain, Khushboo Singh, Jyoti P Singh, Kavita Dubey, Shweta Singh, Gyanendra K Rai, Gyanendra P Singh, Himanshu Pathak, Viswanathan Chinnusamy, Shelly Praveen
Wheat is highly prone to terminal heat stress (HS) under late-sown conditions. Delayed- sowing is one of the preferred methods to screen the genotypes for thermotolerance under open field conditions. We investigated the effect of terminal HS on the thermotolerance of four popular genotypes of wheat i.e. WR544, HD2967, HD2932, and HD2285 under field condition. We observed significant variations in the biochemical parameters like protein content, antioxidant activity, proline and total reducing sugar content in leaf, stem, and spike under normal (26 ± 2°C) and terminal HS (36 ± 2°C) conditions...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28965949/adrenaline-promotes-epithelial-to-mesenchymal-transition-via-hur-tgf%C3%AE-regulatory-axis-in-pancreatic-cancer-cells-and-the-implication-in-cancer-prognosis
#9
Jun Pu, Xiaorui Zhang, Huiwen Luo, Lijuan Xu, Xiaozhao Lu, Jianguo Lu
Psychological stress has recently been described as a risk factor in the development of pancreatic cancer. Here, we reported that increased neurotransmitter adrenaline was associated with the poor survival in pancreatic cancer patients. Moreover, in the cell model study, we found adrenaline promoted pancreatic cell PANC-1 migration in a dose dependent manner. Block of the β2-adrenoreceptor with ICI118,551, significantly reduced cell migration. Further study found that adrenaline induced a cytoplasmic translocation of RNA binding protein HuR, which in turn activated TGFβ, as shown by the SBE luciferase assay and phosphorylation of Smad2/3...
November 25, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28963623/estimation-of-start-and-stop-numbers-for-cluster-resolution-feature-selection-algorithm-an-empirical-approach-using-null-distribution-analysis-of-fisher-ratios
#10
Lawrence A Adutwum, A Paulina de la Mata, Heather D Bean, Jane E Hill, James J Harynuk
Cluster resolution feature selection (CR-FS) is a hybrid feature selection algorithm which involves the evaluation of ranked variables via sequential backward elimination (SBE) and sequential forward selection (SFS). The implementation of CR-FS requires two main inputs, namely, start and stop number. The start number is the number of the highly ranked variables for the SBE while the stop number is the point at which the search for additional features during the SFS stage is halted. The setting of these critical parameters has always relied on trial and error which introduced subjectivity in the results obtained...
September 29, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28945463/attention-deficit-hyperactivity-disorder-symptoms-are-associated-with-overeating-with-and-without-loss-of-control-in-youth-with-overweight-obesity
#11
Amy Heard Egbert, Denise E Wilfley, Kamryn T Eddy, Kerri N Boutelle, Nancy Zucker, Carol B Peterson, Angela Celio Doyle, Daniel Le Grange, Andrea B Goldschmidt
BACKGROUND: There is growing evidence that attention-deficit/hyperactivity disorder (ADHD) and loss of control (LOC) eating, both prevalent in children and adolescents, may be related to one another. However, the relationship between ADHD and overeating without LOC has been largely unexamined, thus precluding an understanding of the independent contributions of LOC and episode size in these associations. The current study sought to examine associations between ADHD symptoms and maladaptive eating by evaluating three different types of eating episodes characterized by the presence/absence of LOC and the amount of food consumed: objectively large LOC episodes [objective binge eating (OBE)], subjectively large binge episodes [subjective binge eating (SBE)], and objectively large overeating episodes without LOC [objective overeating (OO)]...
September 25, 2017: Childhood Obesity
https://www.readbyqxmd.com/read/28938152/comparison-of-manual-and-automated-ampliseq%C3%A2-workflows-in-the-typing-of-a-somali-population-with-the-precision-id-identity-panel
#12
Suzanne van der Heijden, Susanne Juel de Oliveira, Marie-Louise Kampmann, Claus Børsting, Niels Morling
The Precision ID Identity Panel was used to type 109 Somali individuals in order to obtain allele frequencies for the Somali population. These frequencies were used to establish a Somali HID-SNP database, which will be used for the biostatistic calculations in family and immigration cases. Genotypes obtained with the Precision ID Identity Panel were found to be almost in complete concordance with genotypes obtained with the SNPforID PCR-SBE-CE assay. In seven SNP loci, silent alleles were identified, of which most were previously described in the literature...
September 14, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28887709/mapping-and-comparative-proteomic-analysis-of-the-starch-biosynthetic-pathway-in-rice-by-2d-page-ms
#13
Tao-Shan Chang, Chih-Wei Liu, Yu-Ling Lin, Chao-Yi Li, Arthur Z Wang, Min-Wei Chien, Chang-Sheng Wang, Chien-Chen Lai
Our results not only provide a comprehensive overview of the starch biosynthetic pathway in the developing endosperm but also reveal some important protein markers that regulate the synthesis of starch. In human diets, rice (Oryza sativa L.) is an important source of starch, a substantial amount of which is accumulated in developing endosperm. A better understanding of the complicated pathways involved in starch biosynthesis is needed to improve the yield and quality of rice and other cereal crops through breeding...
September 8, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28876987/a-novel-approach-to-simulation-based-education-for-veterinary-medical-communication-training-over-eight-consecutive-pre-clinical-quarters
#14
Ryane E Englar
Experiential learning through the use of standardized patients (SPs) is the primary way by which human medical schools teach clinical communication. The profession of veterinary medicine has followed suit in response to new graduates' and their employers' concerns that veterinary interpersonal skills are weak and unsatisfactory. As a result, standardized clients (SCs) are increasingly relied upon as invaluable teaching tools within veterinary curricula to advance relationship-centered care in the context of a clinical scenario...
2017: Journal of Veterinary Medical Education
https://www.readbyqxmd.com/read/28839463/tgf%C3%AE-1-promotes-gemcitabine-resistance-through-regulating-the-lncrna-let-nf90-mir-145-signaling-axis-in-bladder-cancer
#15
Junlong Zhuang, Lan Shen, Lin Yang, Xiaojing Huang, Qun Lu, Yangyan Cui, Xi Zheng, Xiaozhi Zhao, Dianzheng Zhang, Ruimin Huang, Hongqian Guo, Jun Yan
High tumor recurrence is frequently observed in patients with urinary bladder cancers (UBCs), with the need for biomarkers of prognosis and drug response. Chemoresistance and subsequent recurrence of cancers are driven by a subpopulation of tumor initiating cells, namely cancer stem-like cells (CSCs). However, the underlying molecular mechanism in chemotherapy-induced CSCs enrichment remains largely unclear. In this study, we found that during gemcitabine treatment lncRNA-Low Expression in Tumor (lncRNA-LET) was downregulated in chemoresistant UBC, accompanied with the enrichment of CSC population...
2017: Theranostics
https://www.readbyqxmd.com/read/28828687/regeneration-and-reuse-waste-from-an-edible-oil-refinery
#16
Abdelhamid Boukerroui, Lydia Belhocine, Sonia Ferroudj
A spent bleaching earth (SBE) from an edible oil refinery has been regenerated by thermal processing in oven, followed by washing with a cold solution of hydrochloric acid (1M). Optimal regeneration conditions have been controlled by decolorization tests of degummed and neutralized soybean oil. Optimal values of treatment (temperature 350°C, carbonization time 01 h, and HCl concentration 1M) gave a very efficient material. After bleaching oil by regenerated spent bleaching earth (RSBE), the chlorophyll-a and β-carotenes contained in crude edible oil and observed respectively at 430, 454, and 483 nm, value of λ max, are very much decreased...
August 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28821031/surface-deacetylated-chitin-nanofibers-reinforced-with-a-sulfobutyl-ether-%C3%AE-cyclodextrin-gel-loaded-with-prednisolone-as-potential-therapy-for-inflammatory-bowel-disease
#17
Ryo Tabuchi, Makoto Anraku, Daisuke Iohara, Takako Ishiguro, Shinsuke Ifuku, Tomone Nagae, Kaneto Uekama, Shoko Okazaki, Keizo Takeshita, Masaki Otagiri, Fumitoshi Hirayama
Surface-deacetylated chitin nanofibers (SDACNFs) reinforced with a sulfobutyl ether β-cyclodextrin (SBE-β-CD) (NFs-CDs) gel were developed to obtain a controlled release carrier of prednisolone (PD) for the treatment of colitis. PD was released slowly from the gel at both pH 1.2 and 6.8. The in vitro slow release of PD from the NFs-CDs gel was reflected in the in vivo absorption of the drug after oral administration to rats. These results suggest that a simple gel composed of a mixture of SDACNFs and SBE-β-CD has the potential for use in the controlled release of PD...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28807746/20-hete-regulated-psmb5-expression-via-tgf-%C3%AE-smad-signaling-pathway
#18
Guangrui Lai, Ru Sun, Jingjing Wu, Bijun Zhang, Yanyan Zhao
We previously found that 20-hydroxyeicosatetraeonic acid (20-HETE) showed an effect on proteasome activity in cytochrome P450 F2 (CYP4F2) transgenic mice. Proteasome subunit β5 (PSMB5) is a primary subunit of the proteasome. In the current study, we examine whether 20-HETE has any affect on PSMB5. We found that PSMB5 was upregulated in the liver, but downregulated in the kidney of transgenic mice, when compared with wild-type mice. Luciferase reporter gene experiments and electrophoretic mobility shift assays (EMSA) suggested that Smad3 directly associated with the putative Smad binding element (SBE) of the Psmb5 promoter...
August 11, 2017: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/28800209/using-local-clinical-educators-and-shared-resources-to-deliver-simulation-training-activities-across-rural-and-remote-south-australia-and-south-west-victoria-a-distributed-collaborative-model
#19
Stacey C Masters, Sandi Elliott, Sarah Boyd, James A Dunbar
PROBLEM: There is a lack of access to simulation-based education (SBE) for professional entry students (PES) and health professionals at rural and remote locations. DESIGN: A descriptive study. SETTING: Health and education facilities in regional South Australia and south-west Victoria. KEY MEASURES FOR IMPROVEMENT: Number of training recipients who participated in SBE; geographical distribution and locations where SBE was delivered; number of rural clinical educators providing SBE...
August 11, 2017: Australian Journal of Rural Health
https://www.readbyqxmd.com/read/28780752/regulation-of-smad-mediated-microrna-transcriptional-response-in-ground-squirrels-during-hibernation
#20
Cheng-Wei Wu, Kenneth B Storey
Mammalian hibernation is a state of dormancy that is used by some animals to survive through the unfavorable conditions of winter, and is characterized by coordinated suppression of basal metabolism that is supported by global inhibition of energy/ATP-consuming processes. In this study, we examine the regulation of the anti-proliferatory TGF-β/Smad transcription factor signaling pathway in the liver tissue of the hibernating 13-lined ground squirrel Ictidomys tridecemlineatus. The TGF-β/Smad signaling pathway is known to mediate cell cycle arrest through induction of cell cycle dependent kinase inhibitors, and more recently, has been shown to regulate a wide range of cellular processes via its control of microRNA biosynthesis...
August 5, 2017: Molecular and Cellular Biochemistry
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