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https://www.readbyqxmd.com/read/28523436/hrm-and-snapshot-as-alternative-forensic-snp-genotyping-methods
#1
Bhavik Mehta, Runa Daniel, Dennis McNevin
Single nucleotide polymorphisms (SNPs) have been widely used in forensics for prediction of identity, biogeographical ancestry (BGA) and externally visible characteristics (EVCs). Single base extension (SBE) assays, most notably SNaPshot® (Thermo Fisher Scientific), are commonly used for forensic SNP genotyping as they can be employed on standard instrumentation in forensic laboratories (e.g. capillary electrophoresis). High resolution melt (HRM) analysis is an alternative method and is a simple, fast, single tube assay for low throughput SNP typing...
May 18, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28515655/effect-of-calcium-lactate-on-physico-chemical-characteristics-of-shank-bone-extract
#2
Jung-Seok Choi, Sang-Keun Jin, Yeong-Seok Choi, Jin-Kyu Lee, Ji-Taek Jung, Yang-Il Choi, Hyun-Joo Lee, Jae-Joon Lee
This study was conducted to develop calcium-fortified shank bone extract (SBE) and to determine the effect of adding calcium lactate on physico-chemical characteristics of SBE during cold storage. The following five experiment groups were used: Control (0%, no addition), T1 (0.05% calcium lactate), T2 (0.1% calcium lactate), T3 (0.5% calcium lactate), and T4 (1% calcium lactate). When the concentration of calcium lactate added to the SBE was increased, the pH, redness, and yellowness values were significantly reduced, whereas the salinity, sugar content, and turbidity of SBE were significantly increased...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28511730/simulation-in-canadian-postgraduate-emergency-medicine-training-a-national-survey
#3
Evan Russell, Andrew Koch Hall, Carly Hagel, Andrew Petrosoniak, Jeffrey Damon Dagnone, Daniel Howes
OBJECTIVES: Simulation-based education (SBE) is an important training strategy in emergency medicine (EM) postgraduate programs. This study sought to characterize the use of simulation in FRCPC-EM residency programs across Canada. METHODS: A national survey was administered to residents and knowledgeable program representatives (PRs) at all Canadian FRCPC-EM programs. Survey question themes included simulation program characteristics, the frequency of resident participation, the location and administration of SBE, institutional barriers, interprofessional involvement, content, assessment strategies, and attitudes about SBE...
May 17, 2017: CJEM
https://www.readbyqxmd.com/read/28507311/disruption-of-shh-signaling-cascade-by-sbe-attenuates-lung-cancer-progression-and-sensitizes-ddp-treatment
#4
Jing Du, Weiwei Chen, Lijuan Yang, Juanjuan Dai, Jiwei Guo, Yan Wu, Kaikai Gong, Jian Zhang, Ning Yu, Zhen Xie, Sichuan Xi
Deregulated Sonic Hedgehog (SHH) pathway facilitates the initiation, progression, and metastasis of Non-small cell lung cancer (NSCLC), confers drug resistance and renders a therapeutic interference option to lung cancer patients with poor prognosis. In this study, we screened and evaluated the specificity of a Chinese herb Scutellariabarbata D. Don extraction (SBE) in repressing SHH signaling pathway to block NSCLC progression. Our study confirmed that aberrant activation of the SHH signal pathway conferred more proliferative and invasive phenotypes to human lung cancer cells...
May 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486184/bioaccessibility-of-polycyclic-aromatic-hydrocarbons-in-activated-carbon-or-biochar-amended-vegetated-salix-viminalis-soil
#5
Patryk Oleszczuk, Paulina Godlewska, Danny D Reible, Piotr Kraska
The aim of the present study was to determine the effect of activated carbon (AC) or biochars on the bioaccessibility (Cbioacc) of polycyclic aromatic hydrocarbons (PAHs) in soils vegetated with willow (Salix viminalis). The study determined the effect of willow on the Cbioacc PAHs and the effect of the investigated amendments on changes in dissolved organic carbon (DOC), crop yield and the content of PAHs in plants. PAH-contaminated soil was amended with 2.5 wt% AC or biochar. Samples from individual plots with and without plants were collected at the beginning of the experiment and after 3, 6, 12 and 18 months...
May 6, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28453964/degradation-of-sulfadimethoxine-catalyzed-by-laccase-with-soybean-meal-extract-as-natural-mediator-mechanism-and-reaction-pathway
#6
Shangtao Liang, Qi Luo, Qingguo Huang
Natural laccase-mediator systems have been well recognized as an eco-friendly and energy-saving approach in environmental remediation, whose further application is however limited by the high cost of natural mediators and relatively long treatment time span. This study evaluated the water extract of soybean meal, a low-cost compound system, in mediating the laccase catalyzed degradation of a model contaminant of emerging concern, sulfadimethoxine (SDM), and demonstrated it as a promising alternative mediator for soil and water remediation...
April 23, 2017: Chemosphere
https://www.readbyqxmd.com/read/28432793/single-and-dual-cyclodextrins-systems-for-the-enantiomeric-and-diastereoisomeric-separations-of-structurally-related-dihydropyridone-analogues
#7
Thibaut Delplanques, Rajaa Boulahjar, Julie Charton, Corentin Houze, Michaël Howsam, Anne-Catherine Servais, Marianne Fillet, Emmanuelle Lipka
Cyclodextrin capillary electrophoresis methods (CD-CZE) were developed for complete enantiomeric and diastereoisomeric separations of a series of ten dihydropyridone analogues, of which eight were neutral, one was anionic and one was cationic. Ten different systems comprising one or two cyclodextrins were found to successfully separate the isomers thanks to a screening approach. Among the tested cyclodextrins, HS-γ-CD, either in a single or in a dual system, in a phosphate buffer using capillaries dynamically coated with polyethylene oxide (PEO), and SBE-β-CD, either in a single or in a dual system, in a borate buffer using uncoated capillaries, were the most selective selectors...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28432685/chiral-analysis-of-%C3%AE-diimine-ru-ii-and-fe-ii-complexes-by-capillary-electrophoresis-using-sulfated-cyclodextrins-as-stereoselectors
#8
Petra Sázelová, Dušan Koval, Lukáš Severa, Filip Teplý, Václav Kašička
CE using randomly highly sulfated α-, β-, and γ-CDs (S-α-CD, S-β-CD, S-γ-CD), sulfobutylether-β-CD (SBE-β-CD), single isomer (6-O-sulfo) α-, β-, and γ-CDs, and their derivatives as stereoselectors was applied to chiral analysis of polypyridyl complexes of [Ru(bpy)3 ](2+) , [Ru(phen)3 ](2+) , and [Fe(phen)3 ](2+) (bpy = 2,2'-bipyridine; phen = 1,10 phenanthroline). The best separations of Δ- and Λ-enantiomers of the these complexes with high resolution (up to R1,2  = 7.0) and short analysis times (10-20 min) were achieved in the BGE composed of 22 mM NaOH/35 mM H3 PO4 , pH 2...
April 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28432283/efficacy-and-safety-of-single-balloon-versus-double-balloon-enteroscopy-a-single-center-retrospective-analysis
#9
Zhanjun Lu, Yu Qi, Jianjun Weng, Lei Ma, Xinjian Wan, Rong Wan, Lungen Lu, Hang Zhao
BACKGROUND Single-balloon endoscopy (SBE) has been introduced as a simplified endoscopy technique after the promotion of double-balloon endoscopy (DBE). The difference in clinical performance between DBE and SBE is still not very clear. In this study, we aimed to compare the efficacy and safety between these 2 endoscopic procedures. MATERIAL AND METHODS A total of 173 patients with suspected small bowel disease were enrolled into this study from January 2007 to December 2011. All cases were divided into DBE or SBE groups according to the endoscopic procedures they underwent...
April 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28421099/progress-in-high-amylose-cereal-crops-through-inactivation-of-starch-branching-enzymes
#10
REVIEW
Juan Wang, Pan Hu, Zichun Chen, Qiaoquan Liu, Cunxu Wei
High-amylose cereal starches provide many health benefits for humans. The inhibition or mutation of starch branching enzyme (SBE) genes is an effective method to develop high-amylose cereal crops. This review summarizes the development of high-amylose cereal crops through the inactivation of one or more SBE isoforms or combination with other genes. This review also reveals the causes of increase in amylose content in high-amylose crops. A series of changes, including amylopectin structure, crystalline structure, thermal properties, and hydrolysis properties, occurs as amylose content increases...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28395505/comparison-of-the-efficacy-and-safety-of-single-versus-double-balloon-enteroscopy-performed-by-endoscopist-experts-in-single-balloon-enteroscopy-a-single-center-experience-and-meta-analysis
#11
Tae Jun Kim, Eun Ran Kim, Dong Kyung Chang, Young-Ho Kim, Sung Noh Hong
Background/Aims: Studies concerning the efficacy and safety of single-balloon enteroscopy (SBE) compared with that of double-balloon enteroscopy (DBE) often appear to be conflicting. However, previous studies were performed by endoscopists who were less experienced in SBE compared with DBE. Methods: We performed a retrospective analysis of SBE and DBE data performed by a single enteroscopist, with expertise in SBE, using a prospective balloon-assisted enteroscopy registry from 2013 to 2015...
April 11, 2017: Gut and Liver
https://www.readbyqxmd.com/read/28365205/articular-ultrasound-in-asymptomatic-volunteers-identification-of-the-worst-measures-of-synovial-hypertrophy-synovial-blood-flow-and-joint-damage-among-small-medium-%C3%A2-and-large-sized-joints
#12
Flávia Soares Machado, Jamil Natour, Rogerio Diniz Takahashi, Rita N V Furtado
Articular ultrasound of 6500 joint recesses was performed for the purpose of identifying which joint had the highest measurements among small-sized (SSJ), medium-sized (MSJ) and large-sized (LSJ) joints. Quantitative measurements of synovial hypertrophy (QSR) and semiquantitative measurements of synovial hypertrophy (SSH), power Doppler (SPD) and bone erosion (SBE) (score: 0-3) were made. Higher measurements (p < 0.01) of QSR were obtained in the second metatarsophalangeal joint (MTP), talonavicular joint, and hip...
June 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28358319/effective-subcritical-butane-extraction-of-bifenthrin-residue-in-black-tea
#13
Yating Zhang, Lingbiao Gu, Fei Wang, Lingjun Kong, Guangyong Qin
As a natural and healthy beverage, tea is widely enjoyed; however, the pesticide residues in tea leaves affect the quality and food safety. To develop a highly selective and efficient method for the facile removal of pesticide residues, the subcritical butane extraction (SBE) technique was employed, and three variables involving temperature, time and extraction cycles were studied. The optimum SBE conditions were found to be as follows: extraction temperature 45 °C, extraction time 30 min, number of extraction cycles 1, and in such a condition that the extraction efficiency reached as high as 92%...
March 30, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28326091/generation-of-high-amylose-rice-through-crispr-cas9-mediated-targeted-mutagenesis-of-starch-branching-enzymes
#14
Yongwei Sun, Guiai Jiao, Zupei Liu, Xin Zhang, Jingying Li, Xiuping Guo, Wenming Du, Jinlu Du, Frédéric Francis, Yunde Zhao, Lanqin Xia
Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. However, it remains a challenge to control starch branching in commercial lines. Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in SBEI and SBEIIb in rice. The frequencies of obtained homozygous or bi-allelic mutant lines with indels in SBEI and SBEIIb in T0 generation were from 26...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28299353/safety-and-efficacy-of-single-balloon-enteroscopy-in-management-of-gastrointestinal-bleeding-in-patients-with-a-left-ventricular-assist-device
#15
Abhinav Koul, Donald M Pham, Arjun Nanda, Kevin E Woods, Steven D Keilin
Background and study aims Left ventricular assist devices (LVADs) are currently the standard of care in treatment of patients with end-stage heart failure waiting for heart transplant as well as destination therapy for non-transplant candidates. However, patients with LVADs are at increased risk of gastrointestinal bleeding due to the device's unique effects on hemodynamics. A major source of gastrointestinal bleeding in these patients are gastrointestinal angioectasias located within the small bowel that can only be reached with deep enteroscopy...
March 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28299150/awareness-and-practice-of-self-breast-examination-among-female-nurses-at-the-federal-teaching-hospital-ido-ekiti-nigeria
#16
Olusegun Emmanuel Gabriel, Oluwaserimi Adewumi Ajetunmobi, Olabode Muftau Shabi, Olaide Toyin Elegbede, Raymond Akujuobi Okere, Olusegun Adesola Busari, Ayokunle Samuel Dada
Breast cancer is the most common female malignancy linked with high levels of morbidity and mortality in developing countries due to delayed diagnosis. This research assessed the knowledge of signs and risk factors of breast cancer and practice of self breast examination (SBE) among female nurses in a rural tertiary Hospital. Eighty-five nurses ages 20 to 60 years were sampled by simple randomization over a period of eight weeks through a self-administered semi-structured questionnaire. The analysis was done using statistical package for social science version 17...
August 17, 2016: Journal of Public Health in Africa
https://www.readbyqxmd.com/read/28256567/a-wogonin-rich-fraction-of-scutellaria-baicalensis-root-extract-exerts-chondroprotective-effects-by-suppressing-il-1%C3%AE-induced-activation-of-ap-1-in-human-oa-chondrocytes
#17
Nazir M Khan, Abdul Haseeb, Mohammad Y Ansari, Tariq M Haqqi
Osteoarthritis (OA) is a common joint disorder with varying degrees of inflammation and sustained oxidative stress. The root extract of Scutellaria baicalensis (SBE) has been used for the treatment of inflammatory and other diseases. Here, we performed activity-guided HPLC-fractionation of SBE, identified the active ingredient(s) and investigated its chondroprotective potential. We found that the Wogonin containing fraction-4 (F4) was the most potent fraction based on its ability to inhibit ROS production and the suppression of catabolic markers including IL-6, COX-2, iNOS, MMP-3, MMP-9, MMP-13 and ADAMTS-4 in IL-1β-treated OA chondrocytes...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28248993/role-of-computed-tomography-angiography-on-the-management-of-overt-obscure-gastrointestinal-bleeding
#18
Chao-Ming Tseng, I-Chang Lin, Chi-Yang Chang, Hsiu-Po Wang, Chih-Cheng Chen, Lein-Ray Mo, Jaw-Town Lin, Chi-Ming Tai
BACKGROUND AND AIM: The role of computed tomography angiography (CTA) on the management of acute overt obscure gastrointestinal bleeding (OGIB) remains unclear. We designed a study to evaluate the impact of CTA before enteroscopy for acute overt OGIB. METHODS: All patients undergoing CTA followed by enteroscopy for acute overt OGIB were enrolled in this retrospective study. Clinical characteristics and diagnosis were compared between patients with positive and negative CTA findings...
2017: PloS One
https://www.readbyqxmd.com/read/28216462/a-study-of-the-aggregation-of-cyclodextrins-determination-of-the-critical-aggregation-concentration-size-of-aggregates-and-thermodynamics-using-isodesmic-and-k2-k-models
#19
Thao Thi Do, Rob Van Hooghten, Guy Van den Mooter
The aggregation of three different cyclodextrins (CDs): 2-hydroxypropyl-β-cyclodextrin (HP-β-CD), 2-hydroxypropyl-γ-cyclodextrin (HP-γ-CD) and sulfobutylether-β-cyclodextrin (SBE-β-CD) was studied. The critical aggregation concentration (cac) of these three CDs is quite similar and is situated at ca. 2% (m/v). There was only a small difference in the cac values determined by DLS and (1)H NMR. DLS measurements revealed that CDs in solution have three size populations wherein one of them is that of a single CD molecule...
February 17, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28210706/single-balloon-enteroscopy-efficacy-and-degree-of-concordance-with-noninvasive-evaluation-of-small-bowel
#20
Margarida Marques, João Santos-Antunes, Rosa Coelho, Hélder Cardoso, Filipe Vilas Boas, Armando Ribeiro, Guilherme Macedo
Background and study aims Clinical impact of single-balloon enteroscopy (SBE) is fairly known, as well as its diagnostic yield comparing with other small bowel gastrointestinal investigations. This study represents a contribution to better understand it and is designed to evaluate SBE efficacy and degree of concordance with previous evaluation of small bowel. Patients and methods This is a single-center retrospective study of patients that underwent SBE with suspected small bowel disease based on non-invasive imaging...
February 2017: Endoscopy International Open
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