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Ling-Yun Guo, Si-Kai Ling, Chang-Ming Li, Guan-Jun Chen, Zong-Jun Du
A novel Gram-stain-negative, moderately halophilic, motile, facultatively anaerobic and rod-shaped strain, designated WDN1C137(T), was isolated from a marine saltern at Wendeng, PR China. Optimal growth occurred at 40 °C, pH 7.5 and with 7.0 % (w/v) NaCl. Q-10 was the sole respiratory quinone. The major cellular fatty acids (>10.0 %) in WDN1C137(T) were C18 : 1ω7c (46.2 %), cyclo C19 : 0ω8c (18.7 %) and C16 : 0 (12.3 %). The major polar lipids were phosphatidylglycerol, phosphoglycolipid, phosphatidylcholine, one unidentified glycolipid, one unidentified lipid, one unidentified aminolipid and two unidentified phospholipids...
October 18, 2017: International Journal of Systematic and Evolutionary Microbiology
Wipaporn Ngaemthao, Tawanmol Pujchakarn, Suwanee Chunhametha, Chanwit Suriyachadkun
A novel endophytic filamentous bacterium strain A-T 7972(T), was isolated from wild orchid Grosourdya appendiculata (Blume) Rchb.f. collected in Thailand. The bacterium developed single non-motile spores with warty surface on substrate mycelia. The taxonomic position was described using a polyphasic approach. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 7972(T) belonged to the genus Verrucosispora and shared the highest sequence similarity with V. lutea YIM 013(T) (98.71 %) and V...
October 18, 2017: International Journal of Systematic and Evolutionary Microbiology
Maria Fernanda Pérez Bernal, Elcia Margareth Souza Brito, Manon Bartoli, Johanne Aubé, Marie-Laure Fardeau, German Cuevas Rodriguez, Bernard Ollivier, Rémy Guyoneaud, Agnès Hirschler-Réa
Novel Gram-stain-negative, non-spore-forming, vibrio-shaped, anaerobic, alkaliphilic, sulfate-reducing bacteria, designated strains PAR180(T) and PAR190, were isolated from sediments collected at an alkaline crater lake in Guanajuato (Mexico). Strain PAR180(T) grew at temperatures between 15 and 40 °C (optimum 35 °C), and at pH between 8.3 and 10.4 (optimum 9). It was halotolerant, growing with up to 8 % (w/v) NaCl. Lactate, formate, pyruvate and ethanol were used as electron donors in the presence of sulfate and were incompletely oxidized to acetate and CO2...
October 17, 2017: International Journal of Systematic and Evolutionary Microbiology
Farida El-Baz, Mohammed Abd El-Aal, Tarek Moustafa Kamal, Abdelrahim Abdrabou Sadek, Amr Ahmed Othman
BACKGROUND: Autism is currently known as "a behaviorally defined syndrome" manifested as impairment in social communication, repetitive routines and restricted interests. There is an increased risk of ASDs associated with common mutations affecting the folate/methylation cycle. AIM: The aim of this study was to identify C677T and 1298AC polymorphic genotypes of MTHFR gene among a sample of Egyptian children with autism and to make a phenotype-genotype correlation for the autistic patients...
September 2017: Electronic Physician
Hisami Kobayashi, Takuya Nakasato, Mitsuo Sakamoto, Yoshihisa Ohtani, Fuminori Terada, Ken Sakai, Moriya Ohkuma, Masanori Tohno
A Gram-stain-variable, strictly anaerobic, rod-shaped, catalase-negative and endospore-forming bacterial strain, designated MJC39(T), was isolated from grass silage preserved in Hokkaido, Japan. Growth occurred at 20-42 °C, pH 5.0-7.0 and NaCl concentrations up to 2 % (w/v). The isolated strain MJC39(T) produced butyric acid in peptone yeast extract medium with glucose. The DNA G+C content of strain MJC39(T) was 34.4±0.2 mol%. The major cellular fatty acids (>10 %) were C14 : 0, C16 : 0 and summed feature 3 (including C16 : 1ω7c/C16 : 1ω6c)...
October 16, 2017: International Journal of Systematic and Evolutionary Microbiology
Ziqing Gao, Chenglin Su, Xueying Yang, Di Sun, Chao Zeng, Ming Chen, Wenzhong Hu, Chunzhi Zhang
A novel bacterium, designated strain DL503(T), was isolated from a Daqu sample and its taxonomic position determined using a polyphasic taxonomy. Strain DL503(T) was a Gram-stain-negative, facultatively anaerobic, non-sporulating, motile and coccoid-rod-shaped bacterium. Optimum growth occurred at 20-45 °C, pH 5.0-10.0 and 1.5 % (w/v) NaCl. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belongs to the genus Franconibacter, showing highest levels of similarity with respect to Franconibacter pulveris JCM 16471(T) (98...
October 16, 2017: International Journal of Systematic and Evolutionary Microbiology
An-Zhang Li, Long-Zhen Lin, Ming-Xia Zhang, Hong-Hui Zhu
A novel bacterium, designated R14M6(T), was isolated from deep-sea sediment collected from the Ross Sea, Antarctica. Cells are Gram-stain-negative, aerobic, pale yellow, short-rod-shaped, polar-flagellated and aggregate-forming. Growth occurs at 4-36 °C, pH 6.0-8.3, and in 1-15 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R14M6(T) clustered together with Aurantimonas endophytica EGI6500337(T) and fell within the genus Aurantimonas. The 16S rRNA gene sequence of strain R14M6(T) shared similarity with A...
October 16, 2017: International Journal of Systematic and Evolutionary Microbiology
Alexandra R D'Agostino, Allison R Optican, Shaina J Sowles, Melissa J Krauss, Kiriam Escobar Lee, Patricia A Cavazos-Rehg
BACKGROUND: Social media has increasingly become a venue for health discourse and support, particularly for vulnerable individuals. This study examines user-generated content of an online Reddit community targeting individuals recovering from opiate addiction. METHODS: 100 Reddit posts and their comments were collected from the online community on August 19, 2016. Posts were qualitatively coded for opioid use disorder (OUD) criteria as outlined by the Diagnostic and Statistical Manual of Mental Disorders (DSM-5), as well as other common themes...
October 5, 2017: Drug and Alcohol Dependence
Leonid N Ten, Nabil Elderiny, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Dae Sung Lee, Myung Kyum Kim, Hee-Young Jung
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J8-9(T), was isolated from a sandy beach in Jeju Island, South Korea. The isolate was able to grow between 10 and 30 °C, pH 5-8, and in presence of 0-1% (w/v) NaCl. Based on 16S rRNA gene phylogenetic analysis, the novel strain was closely related to members of the genus Spirosoma (96.1-90.9% similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099(T) (96.1%). The G + C content of the genomic DNA of strain 15J8-9(T) was 45...
October 11, 2017: Current Microbiology
Nicola Warren, Cullen O'Gorman, Alexander Lehn, Dan Siskind
OBJECTIVES: Dopamine dysregulation syndrome (DDS) is an uncommon complication of the treatment of Parkinson's disease, characterised by addictive behaviour and excessive use of dopaminergic medication. DDS may frequently go unrecognised or misdiagnosed. We aimed to clarify current understanding of presentation, risk factors, comorbidities and management of DDS. METHODS: Case reports were identified through a systematic search of databases (PubMed, Embase) with the following terms: dopaminergic dysregulation syndrome, hedonistic homeostatic dysregulation, dopamine/levodopa addiction...
October 10, 2017: Journal of Neurology, Neurosurgery, and Psychiatry
Dawn O'Sullivan, Noeleen Brady, Edmund Manning, Emma O'Shea, Síle O'Grady, Niamh O 'Regan, Suzanne Timmons
Background: screening for cognitive impairment in Emergency Department (ED) requires short, reliable tools. Objective: to validate the 4AT and 6-Item Cognitive Impairment Test (6-CIT) for ED dementia and delirium screening. Design: diagnostic accuracy study. Setting/subjects: attendees aged ≥70 years in a tertiary care hospital's ED. Methods: trained researchers assessed participants using the Standardised Mini Mental State Examination, Delirium Rating Scale-Revised 98 and Informant Questionnaire on Cognitive Decline in the Elderly, informing ultimate expert diagnosis using Diagnostic and Statistical Manual of Mental Disorders (DSM-V) criteria for dementia and delirium (reference standards)...
September 1, 2017: Age and Ageing
Guishan Zhang, Mohamed Fauzi Haroon, Ruifu Zhang, Xiaoyan Dong, Di Liu, Qin Xiong, Weibing Xun, Xiuzhu Dong, Ulrich Stingl
Strain SJ5A-1(T), a Gram-stain-negative, coccus-shaped, non-motile, aerobic bacterium, was isolated from the brine-seawater interface of the Erba Deep in the Red Sea, Saudi Arabia. The colonies of strain SJ5A-1(T) have a beige to pale-brown pigmentation, are approximately 0.5-0.7 µm in diameter, and are catalase and oxidase positive. Growth occurred optimally at 30-33 °C, pH 7.0-7.5, and in the presence of 9.0-12.0 % NaCl (w/v). Phylogenetic analysis of the 16S rRNA gene indicates that strain SJ5A-1(T) is a member of the genus Ponticoccus within the family Rhodobacteraceae...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Shin Ae Lee, Jeong Myeong Kim, Hayoung Cho, Soo-Jin Kim, Jae-Hyung Ahn, Moriyuki Hamada, Soon-Wo Kwon, Hang-Yeon Weon
A novel actinomycete strain, designated KIS14-16(T), was isolated from forest soil in Ongjin county, South Korea and characterized using polyphasic taxonomy. The cells are aerobic, Gram-stain-positive, non-flagellated and short rods. The strain grew in a temperature range of 4-33 °C (optimum, 28-30 °C) and pH range of 5.0-10.0 (optimum, 7.0) and in the presence of 0-5 % (w/v) NaCl (optimum, 0 %). Comparison of 16S rRNA gene sequences showed that strain KIS14-16(T) is a member of the genus Arthrobacter exhibiting high sequence similarity with A...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Alberto Enrique Paniz-Mondolfi, Alexander L Greninger, Lynn Ladutko, Barbara A Brown-Elliott, Ravikiran Vasireddy, Wesley Jakubiec, Sruthi Vasireddy, Richard J Wallace, Keith E Simmon, Bruce E Dunn, Gary Jackoway, Surabhi B Vora, Kevin K Quinn, Xuan Qin, Sheldon Campbell
A previously undescribed, rapidly growing, scotochromogenic species of the genus Mycobacterium (represented by strains PB739(T) and GK) was isolated from two clinical sources - the sputum of a 76-year-old patient with severe chronic obstructive pulmonary disease, history of tuberculosis exposure and Mycobacterium avium complex isolated years prior; and the blood of a 15-year-old male with B-cell acute lymphoblastic leukaemia status post bone marrow transplant. The isolates grew as dark orange colonies at 25-37 °C after 5 days, sharing features in common with other closely related species...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Ilaria Finore, Alessia Gioiello, Luigi Leone, Pierangelo Orlando, Ida Romano, Barbara Nicolaus, Annarita Poli
A Gram-stain-positive, aerobic, endospore-forming, thermophilic bacterium, strain N.8(T), was isolated from the curing step of an olive mill pomace compost sample, collected at the Composting Experimental Centre (CESCO, Salerno, Italy). Strain N.8(T), based on 16S rRNA gene sequence similarities, was most closely related to Aeribacillus pallidus strain H12(T) (=DSM 3670(T)) (99.8 % similarity value) with a 25 % DNA-DNA relatedness value. Cells were rod-shaped, non-motile and grew optimally at 60 °C and pH 9...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Inam Ullah Khan, Firasat Hussain, Neeli Habib, Mohammed A M Wadaan, Iftikhar Ahmed, Wan-Taek Im, Wael N Hozzein, Xiao-Yang Zhi, Wen-Jun Li
A novel bacterial strain, designated YIM 73061(T), was isolated from the Cholistan desert in Punjab, Pakistan, and characterized by using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Phenylobacterium conjunctum FWC21(T) (97.6 %), Phenylobacterium lituiforme FaiI3(T) (97.4 %), Phenylobacteriumcomposti 4T-6(T) (97.0 %) and Phenylobacterium aquaticum W2-3-4(T) (96.8 %). Cells were Gram-stain-negative, aerobic and motile rods that formed orange colonies...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
N Kacenelenbogen
CONTEXT: in Belgium 70 % of the children aged 0 - 14 years have at least one annual contact with their family doctor, while for the same period, only 6 - 12 % of them will see a neuropaediatrician and/or a child psychiatrist, despite the fact that a diagnostic of Externalizing behavior before the age of 6 to 7 years, such as various ways of aggressiveness, of anger or of stealing will very often alert the family, the staff of creches or nursery as well as the teachers. Other children are showing signs of Internalizing behavior, very often together with depression or other forms of anxiety...
2017: Revue Médicale de Bruxelles
Yulia Boltyanskaya, Ekaterina Detkova, Nikolay Pimenov, Vadim Kevbrin
A search for the organisms responsible for the degradation of biomass of primary producers in Tanatar lakes resulted in the isolation of a novel anaerobic, haloalkaliphilic microorganism, strain Z-710(T). The strain grows on proteinaceous substrates (peptides) but not on proteins. A rather limited range of substances of other classes can be utilised together with tryptone but not individually. An interesting physiological feature of the novel strain is a high capacity for hydrogen production (up to 30% v/v) during proteolytic fermentation...
October 3, 2017: Antonie Van Leeuwenhoek
Junli Zhuang, Di Xin, Yu-Qin Zhang, Jingnan Guo, Jianli Zhang
A novel bacterial strain, designed Q4-3(T), was isolated from a soil sample obtained from Qilian grassland, Qinghai, China. Phylogenetic, phenotypic, chemotaxonomic and molecular analyses were performed on the new isolate. Cells were Gram-stain-positive, facultatively anaerobic, spore-forming, motile rods with peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences placed strain Q4-3(T) in the genus Paenibacillus, and its closest relatives were Paenibacillus odorifer JCM 21743(T), Paenibacillus typhae DSM 25190(T), Paenibacillus borealis DSM 13188(T) and Paenibacillus etheri DSM 29760(T) with 16S rRNA gene sequence similarities of 98...
September 27, 2017: International Journal of Systematic and Evolutionary Microbiology
Ashish Verma, Yash Pal, Indu Khatri, Anup Kumar Ojha, Harald Gruber-Vodicka, Peter Schumann, Syed Dastager, Srikrishna Subramanian, Shanmugam Mayilraj, Srinivasan Krishnamurthi
Two novel Gram-staining positive, rod-shaped, moderately halotolerant, endospore forming bacterial strains 5.5LF 38TD and 5.5LF 48TD were isolated and taxonomically characterized from a landfill in Chandigarh, India. The analysis of 16S rRNA gene sequences of the strains confirmed their closest identity to Bacillus thermotolerans SgZ-8T with 99.9% sequence similarity. A comparative phylogenetic analysis of strains 5.5LF 38TD, 5.5LF 48TD and B. thermotolerans SgZ-8(T) confirmed their separation into a novel genus with B...
September 12, 2017: Systematic and Applied Microbiology
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