Douglas Thomas, Marianne Palczewski, Hannah Kuschman, Brian Hoffman, Hao Yang, Sharon Glynn, David Wilson, Eric Kool, William Montfort, Jenny Chang, Aydolun Petenkaya, Constantinos Chronis, Thomas Cundari, Sushma Sappa, Kabirul Islam, Daniel McVicar, Yu Fan, Qingrong Chen, Daoud Meerzaman, Michael Sierk
DNA methylation at cytosine bases of eukaryotic DNA (5-methylcytosine, 5mC) is a heritable epigenetic mark that can regulate gene expression in health and disease. Enzymes that metabolize 5mC have been well-characterized, yet the discovery of endogenously produced signaling molecules that regulate DNA methyl-modifying machinery have not been described. Herein, we report that the free radical signaling molecule nitric oxide (NO) can directly inhibit the Fe(II)/2-OG-dependent DNA demethylases ten-eleven translocation (TET) and human AlkB homolog 2 (ALKBH2)...
April 3, 2024: Research Square