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https://www.readbyqxmd.com/read/28209915/exclusive-destruction-of-mitotic-spindles-in-human-cancer-cells
#1
Leonid Visochek, Asher Castiel, Leonid Mittelman, Michael Elkin, Dikla Atias, Talia Golan, Shai Izraeli, Tamar Peretz, Malka Cohen-Armon
We identified target proteins modified by phenanthrenes that cause exclusive eradication of human cancer cells. The cytotoxic activity of the phenanthrenes in a variety of human cancer cells is attributed by these findings to post translational modifications of NuMA and kinesins HSET/kifC1 and kif18A. Their activity prevented the binding of NuMA to α-tubulin and kinesins in human cancer cells, and caused aberrant spindles. The most efficient cytotoxic activity of the phenanthridine PJ34, caused significantly smaller aberrant spindles with disrupted spindle poles and scattered extra-centrosomes and chromosomes...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28209731/transport-of-fungal-rab11-secretory-vesicles-involves-myosin-5-dynein-dynactin-p25-and-kinesin-1-and-is-independent-of-kinesin-3
#2
Miguel A Peñalva, Jun Zhang, Xin Xiang, Areti Pantazopoulou
Hyphal tip cells of the fungus Aspergillus nidulans are useful to study long-range intracellular traffic. Post-Golgi secretory vesicles (SVs) containing the RAB11 orthologue RabE engage myosin-5 as well as plus-end- and minus-end-directed microtubule motors, providing an experimental system to investigate the interplay between microtubule and actin motors acting on the same cargo. By exploiting the fact that depolymerization of F-actin 'unleashes' SVs focused at the apex by myosin-5 to microtubule-dependent motors, we establish that the minus-end directed transport of SVs requires the dynein/dynactin supercomplex...
February 16, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28208320/dynamic-model-for-kinesin-mediated-long-range-transport-and-its-local-traffic-jam-caused-by-tau-proteins
#3
Woochul Nam, Bogdan I Epureanu
In neurons, several intracellular cargoes are transported by motor proteins (kinesins) which walk on microtubules (MTs). However, kinesins can possibly unbind from the MTs before they reach their destinations. The unbound kinesins randomly diffuse in neurons until they bind to MTs. Then, they walk again along the MTs to continue their tasks. Kinesins repeat this cycle of motion until they transport their cargoes to the destinations. However, most previous models mainly focused on the motion of kinesins when they walk on MTs...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28203733/kinesins-in-spermatogenesis%C3%A2
#4
Dan-Dan Ma, Da-Hui Wang, Wan-Xi Yang
No abstract text is available yet for this article.
February 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28202611/yeast-kinesin-5-clusters-to-switch-directionality
#5
(no author information available yet)
No abstract text is available yet for this article.
February 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28195215/the-structural-switch-of-nucleotide-free-kinesin
#6
Luyan Cao, Soraya Cantos-Fernandes, Benoît Gigant
Kinesin-1 is an ATP-dependent motor protein that moves towards microtubules (+)-ends. Whereas structures of isolated ADP-kinesin and of complexes with tubulin of apo-kinesin and of ATP-like-kinesin are available, structural data on apo-kinesin-1 in the absence of tubulin are still missing, leaving the role of nucleotide release in the structural cycle unsettled. Here, we identified mutations in the kinesin nucleotide-binding P-loop motif that interfere with ADP binding. These mutations destabilize the P-loop (T87A mutant) or magnesium binding (T92V), highlighting a dual mechanism for nucleotide release...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28189052/a-novel-zea-mays-ssp-mexicana-l-myc-type-ice-like-transcription-factor-gene-zmmice1-enhances-freezing-tolerance-in-transgenic-arabidopsis-thaliana
#7
Xiang Lu, Lei Yang, Mengyuan Yu, Jianbin Lai, Chao Wang, David McNeil, Meixue Zhou, Chengwei Yang
The annual Zea mays ssp. mexicana L., a member of the teosinte group, is a close wild relative of maize and thus can be effectively used in maize improvement. In this study, an ICE-like gene, ZmmICE1, was isolated from a cDNA library of RNA-Seq from cold-treated seedling tissues of Zea mays ssp. mexicana L. The deduced protein of ZmmICE1 contains a highly conserved basic helix-loop-helix (bHLH) domain and C-terminal region of ICE-like proteins. The ZmmICE1 protein localizes to the nucleus and shows sumoylation when expressed in an Escherichia coli reconstitution system...
February 5, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28183295/meta-analysis-of-gene-expression-in-relapsed-childhood-b-acute-lymphoblastic-leukemia
#8
Yock-Ping Chow, Hamidah Alias, Rahman Jamal
BACKGROUND: Relapsed pediatric B-acute lymphoblastic leukemia (B-ALL) remains as the leading cause of cancer death among children. Other than stem cell transplantation and intensified chemotherapy, no other improved treatment strategies have been approved clinically. Gene expression profiling represents a powerful approach to identify potential biomarkers and new therapeutic targets for various diseases including leukemias. However, inadequate sample size in many individual experiments has failed to provide adequate study power to yield translatable findings...
February 10, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28178520/identification-of-a-sgo2-dependent-but-mad2-independent-pathway-controlling-anaphase-onset-in-fission-yeast
#9
John C Meadows, Theresa C Lancaster, Graham J Buttrick, Alicja M Sochaj, Liam J Messin, Maria Del Mar Mora-Santos, Kevin G Hardwick, Jonathan B A Millar
The onset of anaphase is triggered by activation of the anaphase-promoting complex/cyclosome (APC/C) following silencing of the spindle assembly checkpoint (SAC). APC/C triggers ubiquitination of Securin and Cyclin B, which leads to loss of sister chromatid cohesion and inactivation of Cyclin B/Cdk1, respectively. This promotes relocalization of Aurora B kinase and other components of the chromosome passenger complex (CPC) from centromeres to the spindle midzone. In fission yeast, this is mediated by Clp1 phosphatase-dependent interaction of CPC with Klp9/MKLP2 (kinesin-6)...
February 7, 2017: Cell Reports
https://www.readbyqxmd.com/read/28165699/eg5-inhibitors-have-contrasting-effects-on-microtubule-stability-and-metaphase-spindle-integrity
#10
Geng-Yuan Chen, You Jung Kang, A Sophia Gayek, Wiphu Youyen, Erkan Tuzel, Ryoma Ohi, William O Hancock
To uncover their contrasting mechanisms, antimitotic drugs that inhibit Eg5 (kinesin-5) were analyzed in mixed-motor gliding assays of kinesin-1 and Eg5 motors in which Eg5 "braking" dominates motility. Loop-5 inhibitors (monastrol, STLC, ispinesib and filanesib) increased gliding speeds, consistent with inducing a weak-binding state in Eg5, whereas BRD9876 slowed gliding, consistent with locking Eg5 in a rigor state. Biochemical and single-molecule assays demonstrated that BRD9876 acts as an ATP- and ADP-competitive inhibitor with 4 nM KI...
February 6, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28165566/high-expression-of-kif14-is-associated-with-poor-prognosis-in-patients-with-epithelial-ovarian-cancer
#11
H-L Qiu, S-Z Deng, C Li, Z-N Tian, X-Q Song, G-D Yao, J-S Geng
OBJECTIVE: Kinesin family member 14 (KIF14) is a mitotic kinesin and plays an important role in tumor progression. KIF14 overexpression has been observed in multiple cancers and has been correlated with a poor prognosis. However, its protein expression and prognostic significance in epithelial ovarian cancer (EOC) remain unclear. In this research, we aimed to explore the relationship of KIF14 expression with clinicopathological parameters and prognosis in EOC. MATERIALS AND METHODS: In this study, we measured KIF14 expression in 170 EOC carcinoma tissue samples with immunohistochemistry and correlated these data with clinicopathological characteristics...
January 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28161539/a-novel-role-of-kif3b-in-the-seminoma-cell-cycle
#12
Hao-Qing Shen, Yu-Xi Xiao, Zhen-Yu She, Fu-Qing Tan, Wan-Xi Yang
KIF3b is a protein of the kinesin-2 family which plays an important role in intraflagellar transport. Testis cancer is a common cancer among young men. Its diagnostic rate is increasing and over half of the cases are seminomas. Many aspects of the mechanism and gene expression background of this cancer remain unclear. Using western-blotting and semi-quantitative PCR we found high protein levels of KIF3b enrichment in seminoma tissue despite the mRNA levels remaining equivalent to that of normal testicular tissues...
February 1, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28160078/proteomic-analysis-of-bmn-cell-lipid-rafts-reveals-roles-in-bombyx-mori-nucleopolyhedrovirus-infection
#13
Xiaolong Hu, Min Zhu, Zi Liang, Dhiraj Kumar, Fei Chen, Liyuan Zhu, Sulan Kuang, Renyu Xue, Guangli Cao, Chengliang Gong
The mechanism of how Bombyx mori nucleopolyhedrovirus (BmNPV) enters cells is unknown. The primary components of membrane lipid rafts are proteins and cholesterol, and membrane lipid rafts are thought to be an active region for host-viral interactions. However, whether they contribute to the entry of BmNPV into silkworm cells remains unclear. In this study, we explored the membrane protein components of lipid rafts from BmN cells with mass spectrometry (MS). Proteins and cholesterol were investigated after establishing infection with BmNPV in BmN cells...
February 3, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28150392/loss-of-function-mutations-in-kif15-underlying-a-braddock-carey-genocopy
#14
Patrick M A Sleiman, Michael March, Kenny Nguyen, Lifeng Tian, Renata Pellegrino, Cuiping Hou, Walid Dridi, Mohamed Sager, Yousef H Housawi, Hakon Hakonarson
Braddock-Carey Syndrome (BCS) is characterized by microcephaly, congenital thrombocytopenia the Pierre-Robin sequence (PRS) and agenesis of the corpus callosum. BCS has been shown to be caused by a 21q22.11 microdeletion that encompasses multiple genes. Here we report a BCS genocopy characterized by congenital thrombocytopenia and PRS that is caused by a loss of function mutation in KIF15 in a consanguineous Saudi Arabian family. Mutations of mitotic kinesins are a well established cause of microcephaly. To our knowledge KIF15 is the first kinesin to be associated with congenital thrombocytopenia...
February 1, 2017: Human Mutation
https://www.readbyqxmd.com/read/28150041/evaluation-of-a-hypothetical-protein-for-serodiagnosis-and-as-a-potential-marker-for-post-treatment-serological-evaluation-of-tegumentary-leishmaniasis-patients
#15
Mariana Pedrosa Lima, Lourena Emanuele Costa, Mariana Costa Duarte, Daniel Menezes-Souza, Beatriz Cristina Silveira Salles, Thaís Teodoro de Oliveira Santos, Fernanda Fonseca Ramos, Miguel Angel Chávez-Fumagalli, Amanda Christine Silva Kursancew, Roberta Passamani Ambrósio, Bruno Mendes Roatt, Ricardo Andrez Machado-de-Ávila, Denise Utsch Gonçalves, Eduardo Antonio Ferraz Coelho
The serodiagnosis for tegumentary leishmaniasis (TL) presents problems related to the sensitivity and/or specificity of the tests. In the present study, an enzyme-linked immunosorbent assay (ELISA) technique was used to evaluate the performance from a Leishmania braziliensis hypothetical protein, LbHyM, in an attempt to compare its serological reactivity with a soluble Leishmania antigenic preparation (SLA) for the serodiagnosis of cutaneous (CL) and mucosal (ML) leishmaniasis. LbHyM was predicted to be a kinesin-like protein by bioinformatics tools...
February 1, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28135059/multicomponent-assembly-of-the-kinesin-spindle-protein-ksp-inhibitor-cpuyj039-and-analogs-as-antimitotic-agents
#16
Carlos Carbajales, Junichi Sawada, Giovanni Marzaro, Eddy Sotelo, Luz Escalante, Antonio Sánchez-D Marta, Xerardo García-Mera, Akira Asai, Alberto Coelho
The potent KSP-inhibitor CPUYJ039 and a set of analogs were prepared by a target-oriented approach, based on a Ugi reaction that uses 2-nitrophenyl isocyanides as key building blocks. The herein documented strategy provides a straightforward and atom economical access to potent benzimidazole-based antimitotic agents by exploting the versatility and exploratory power of the Ugi reaction. The results of docking studies and biological activity evaluations of the benzimidazole compounds are also reported.
January 30, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28134340/kif13b-establishes-a-cav1-enriched-microdomain-at-the-ciliary-transition-zone-to-promote-sonic-hedgehog-signalling
#17
Kenneth B Schou, Johanne B Mogensen, Stine K Morthorst, Brian S Nielsen, Aiste Aleliunaite, Andrea Serra-Marques, Nicoline Fürstenberg, Sophie Saunier, Albane A Bizet, Iben R Veland, Anna Akhmanova, Søren T Christensen, Lotte B Pedersen
Ciliary membrane composition is controlled by transition zone (TZ) proteins such as RPGRIP1, RPGRIPL and NPHP4, which are vital for balanced coordination of diverse signalling systems like the Sonic hedgehog (Shh) pathway. Activation of this pathway involves Shh-induced ciliary accumulation of Smoothened (SMO), which is disrupted by disease-causing mutations in TZ components. Here we identify kinesin-3 motor protein KIF13B as a novel member of the RPGRIP1N-C2 domain-containing protein family and show that KIF13B regulates TZ membrane composition and ciliary SMO accumulation...
January 30, 2017: Nature Communications
https://www.readbyqxmd.com/read/28132815/shortening-of-microtubule-overlap-regions-defines-membrane-delivery-sites-during-plant-cytokinesis
#18
Jeroen de Keijzer, Henk Kieft, Tijs Ketelaar, Gohta Goshima, Marcel E Janson
Different from animal cells that divide by constriction of the cortex inward, cells of land plants divide by initiating a new cell-wall segment from their center. For this, a disk-shaped, membrane-enclosed precursor termed the cell plate is formed that radially expands toward the parental cell wall [1-3]. The synthesis of the plate starts with the fusion of vesicles into a tubulo-vesicular network [4-6]. Vesicles are putatively delivered to the division plane by transport along microtubules of the bipolar phragmoplast network that guides plate assembly [7-9]...
January 25, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28125646/kif4-is-essential-for-mouse-oocyte-meiosis
#19
Nicole J Camlin, Eileen A McLaughlin, Janet E Holt
Progression through the meiotic cell cycle must be strictly regulated in oocytes to generate viable embryos and offspring. During mitosis, the kinesin motor protein Kif4 is indispensable for chromosome condensation and separation, midzone formation and cytokinesis. Additionally, the bioactivity of Kif4 is dependent on phosphorylation via Aurora Kinase B and Cdk1, which regulate Kif4 function throughout mitosis. Here, we examine the role of Kif4 in mammalian oocyte meiosis. Kif4 localized in the cytoplasm throughout meiosis I and II, but was also observed to have a dynamic subcellular distribution, associating with both microtubules and kinetochores at different stages of development...
2017: PloS One
https://www.readbyqxmd.com/read/28123706/interaction-of-fun14-domain-containing-1-a-mitochondrial-outer-membrane-protein-with-kinesin-light-chain-1-via-the-tetratricopeptide-repeat-domain
#20
Won Hee Jang, Young Joo Jeong, Sun Hee Choi, Sang-Hwa Urm, Dae-Hyun Seog
Kinesin 1 is a member of the kinesin superfamily proteins (KIFs) of microtubule-dependent molecular motor proteins that transport organelles and protein complexes in cells. Kinesin 1 consists of a homo- or hetero-dimer of kinesin heavy chains (KHCs), often, although not always, associated with two kinesin light chains (KLCs). KLCs are non-motor proteins that associate with many different binding proteins and cargoes, but their binding partners have not yet been fully identified. In the present study, a yeast two-hybrid system was used to identify proteins that interact with the tetratricopeptide repeat (TPR) domain of KLC1...
January 2017: Biomedical Reports
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