keyword
MENU ▼
Read by QxMD icon Read
search

Kinesin

keyword
https://www.readbyqxmd.com/read/28720664/dynein-efficiently-navigates-the-dendritic-cytoskeleton-to-drive-the-retrograde-trafficking-of-bdnf-trkb-signaling-endosomes
#1
Swathi Ayloo, Pedro Guedes-Dias, Amy E Ghiretti, Erika L F Holzbaur
The efficient transport of cargoes within axons and dendrites is critical for neuronal function. While we have a basic understanding of axonal transport, much less is known about transport in dendrites. We employed an optogenetic approach to recruit motor proteins to cargo in real time within axons or dendrites in hippocampal neurons. Kinesin-1, a robust axonal motor, moves cargo less efficiently in dendrites. In contrast, cytoplasmic dynein efficiently navigates both axons and dendrites; in both compartments, dynamic microtubule plus-ends enhance dynein-dependent transport...
July 18, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28716924/insights-into-the-red-algae-and-eukaryotic-evolution-from-the-genome-of-porphyra-umbilicalis-bangiophyceae-rhodophyta
#2
Susan H Brawley, Nicolas A Blouin, Elizabeth Ficko-Blean, Glen L Wheeler, Martin Lohr, Holly V Goodson, Jerry W Jenkins, Crysten E Blaby-Haas, Katherine E Helliwell, Cheong Xin Chan, Tara N Marriage, Debashish Bhattacharya, Anita S Klein, Yacine Badis, Juliet Brodie, Yuanyu Cao, Jonas Collén, Simon M Dittami, Claire M M Gachon, Beverley R Green, Steven J Karpowicz, Jay W Kim, Ulrich Johan Kudahl, Senjie Lin, Gurvan Michel, Maria Mittag, Bradley J S C Olson, Jasmyn L Pangilinan, Yi Peng, Huan Qiu, Shengqiang Shu, John T Singer, Alison G Smith, Brittany N Sprecher, Volker Wagner, Wenfei Wang, Zhi-Yong Wang, Juying Yan, Charles Yarish, Simone Zäuner-Riek, Yunyun Zhuang, Yong Zou, Erika A Lindquist, Jane Grimwood, Kerrie W Barry, Daniel S Rokhsar, Jeremy Schmutz, John W Stiller, Arthur R Grossman, Simon E Prochnik
Porphyra umbilicalis (laver) belongs to an ancient group of red algae (Bangiophyceae), is harvested for human food, and thrives in the harsh conditions of the upper intertidal zone. Here we present the 87.7-Mbp haploid Porphyra genome (65.8% G + C content, 13,125 gene loci) and elucidate traits that inform our understanding of the biology of red algae as one of the few multicellular eukaryotic lineages. Novel features of the Porphyra genome shared by other red algae relate to the cytoskeleton, calcium signaling, the cell cycle, and stress-tolerance mechanisms including photoprotection...
July 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28715414/kinesin-1-promotes-chondrocyte-maintenance-during-skeletal-morphogenesis
#3
Adrian Santos-Ledo, Marina Garcia-Macia, Philip D Campbell, Marta Gronska, Florence L Marlow
During skeletal morphogenesis diverse mechanisms are used to support bone formation. This can be seen in the bones that require a cartilage template for their development. In mammals the cartilage template is removed, but in zebrafish the cartilage template persists and the bone mineralizes around the cartilage scaffold. Remodeling of unmineralized cartilage occurs via planar cell polarity (PCP) mediated cell rearrangements that contribute to lengthening of elements; however, the mechanisms that maintain the chondrocyte template that supports perichondral ossification remain unclear...
July 17, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28707220/application-of-quasi-steady-state-methods-to-nonlinear-models-of-intracellular-transport-by-molecular-motors
#4
Cole Zmurchok, Tim Small, Michael J Ward, Leah Edelstein-Keshet
Molecular motors such as kinesin and dynein are responsible for transporting material along microtubule networks in cells. In many contexts, motor dynamics can be modelled by a system of reaction-advection-diffusion partial differential equations (PDEs). Recently, quasi-steady-state (QSS) methods have been applied to models with linear reactions to approximate the behaviour of the full PDE system. Here, we extend this QSS reduction methodology to certain nonlinear reaction models. The QSS method relies on the assumption that the nonlinear binding and unbinding interactions of the cellular motors occur on a faster timescale than the spatial diffusion and advection processes...
July 13, 2017: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/28702760/regulation-of-long-distance-transport-of-mitochondria-along-microtubules
#5
REVIEW
Anna Melkov, Uri Abdu
Mitochondria are cellular organelles of crucial importance, playing roles in cellular life and death. In certain cell types, such as neurons, mitochondria must travel long distances so as to meet metabolic demands of the cell. Mitochondrial movement is essentially microtubule (MT) based and is executed by two main motor proteins, Dynein and Kinesin. The organization of the cellular MT network and the identity of motors dictate mitochondrial transport. Tight coupling between MTs, motors, and the mitochondria is needed for the organelle precise localization...
July 12, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28701465/the-yeast-kinesin-5-cin8-interacts-with-the-microtubule-in-a-noncanonical-manner
#6
Kayla M Bell, Hyo Keun Cha, Charles V Sindelar, Jared C Cochran
Kinesin motors play central roles in establishing and maintaining the mitotic spindle during cell division. Unlike most other kinesins, Cin8, a kinesin-5 motor in Saccharomyces cerevisiae, can move bidirectionally along microtubules, switching directionality according to biochemical conditions, a behavior that remains largely unexplained. To this end, we used biochemical rate and equilibrium constant measurements as well as cryo-electron microscopy methodologies to investigate the microtubule interactions of the Cin8 motor domain...
July 12, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28699205/native-kinesin-1-does-not-bind-preferentially-to-gtp-tubulin-rich-microtubules-in-vitro
#7
Qiaochu Li, Stephen J King, Jing Xu
Molecular motors such as kinesin-1 work in small teams to actively shuttle cargos in cells, for example in polarized transport in axons. Here we examined the potential regulatory role of the nucleotide state of tubulin on the run length of cargos carried by multiple kinesin motors, using an optical trapping-based in vitro assay. Based on a previous report that kinesin binds preferentially to GTP-tubulin-rich microtubules, we anticipated that multiple-kinesin cargos would run substantially greater distances along GMPCPP microtubules than along GDP microtubules...
July 12, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/28697331/a-tubulin-binding-switch-underlies-kip3-kinesin-8-depolymerase-activity
#8
Hugo Arellano-Santoyo, Elisabeth A Geyer, Ema Stokasimov, Geng-Yuan Chen, Xiaolei Su, William Hancock, Luke M Rice, David Pellman
Kinesin-8 motors regulate the size of microtubule structures, using length-dependent accumulation at the plus end to preferentially disassemble long microtubules. Despite extensive study, the kinesin-8 depolymerase mechanism remains under debate. Here, we provide evidence for an alternative, tubulin curvature-sensing model of microtubule depolymerization by the budding yeast kinesin-8, Kip3. Kinesin-8/Kip3 uses ATP hydrolysis, like other kinesins, for stepping on the microtubule lattice, but at the plus end Kip3 undergoes a switch: its ATPase activity is suppressed when it binds tightly to the curved conformation of tubulin...
July 10, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28694425/insight-into-microtubule-disassembly-by-kinesin-13s-from-the-structure-of-kif2c-bound-to-tubulin
#9
Weiyi Wang, Soraya Cantos-Fernandes, Yuncong Lv, Hureshitanmu Kuerban, Shoeb Ahmad, Chunguang Wang, Benoît Gigant
Kinesin-13s are critical microtubule regulators which induce microtubule disassembly in an ATP dependent manner. To clarify their mechanism, we report here the crystal structure of a functional construct of the kinesin-13 Kif2C/MCAK in an ATP-like state and bound to the αβ-tubulin heterodimer, a complex mimicking the species that dissociates from microtubule ends during catalytic disassembly. Our results picture how Kif2C stabilizes a curved tubulin conformation. The Kif2C α4-L12-α5 region undergoes a remarkable 25° rotation upon tubulin binding to target the αβ-tubulin hinge...
July 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28692749/pick-and-place-assembly-of-single-microtubules
#10
Mehmet Cagatay Tarhan, Ryuji Yokokawa, Laurent Jalabert, Dominique Collard, Hiroyuki Fujita
Intracellular transport is affected by the filament network in the densely packed cytoplasm. Biophysical studies focusing on intracellular transport based on microtubule-kinesin system frequently use in vitro motility assays, which are performed either on individual microtubules or on random (or simple) microtubule networks. Assembling intricate networks with high flexibility requires the manipulation of 25 nm diameter microtubules individually, which can be achieved through the use of pick-and-place assembly...
July 10, 2017: Small
https://www.readbyqxmd.com/read/28685905/3d-modelling-of-carboxyl-terminal-phosphorylation-of-plant-%C3%AE-%C3%AE-tubulin-and-its-role-in-kinesin-8-microtubule-interaction
#11
O M Demchuk, P A Karpov, Ya B Blume
The results of computer modelling of plant kinesin-8/αβ-tubulin complexes with such αβ-tubulins' modified amino acid residues as phosphorylated Tyr262 and Tyr107 are reported in this paper. The molecular dynamics of these modified complexes in comparison with the dynamics of non-modified ones suggests that the phosphorylation of both α- and β-tubulins reveals stabilizing effect on the ptotein structure around the modified residue. It was found also that the phosphorylation of Tyr107 in β-tubulin molecule favours to more advantageous kinesin-8 binding with the phosphorylated microtubule surface in terms of energy...
July 7, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28684886/eg5-overexpression-is-predictive-of-poor-prognosis-in-hepatocellular-carcinoma-patients
#12
Can Liu, Nan Zhou, Jieying Li, Jun Kong, Xi Guan, Xudong Wang
Eg5 (kinesin spindle protein) plays an essential role in mitosis. Inhibition of Eg5 function results in cell cycle arrest at mitosis, which leads to cell death. To date, Eg5 expression and its prognostic significance have not been studied in hepatocellular carcinoma (HCC). In this study, 26 freshly frozen HCC tissue samples and matched peritumoral tissue samples were evaluated with a one-step qPCR test and immunohistochemical (IHC) analysis was conducted on 156 HCC samples to investigate the relationships among Eg5 expression, clinicopathological factors, and prognosis...
2017: Disease Markers
https://www.readbyqxmd.com/read/28678816/characterization-of-kinesin-switch-i-mutations-that-cause-hereditary-spastic-paraplegia
#13
Scott Jennings, Madeline Chenevert, Liqiong Liu, Madhusoodanan Mottamal, Edward J Wojcik, Thomas M Huckaba
Kif5A is a neuronally-enriched isoform of the Kinesin-1 family of cellular transport motors. 23 separate mutations in the motor domain of Kif5A have been identified in patients with the complicated form of hereditary spastic paraplegia (HSP). We performed in vitro assays on dimeric recombinant Kif5A with HSP-causing mutations in the Switch I domain, which participates in the coordination and hydrolysis of ATP by kinesin. We observed a variety of significantly reduced catalytic and mechanical activities as a result of each mutation, with the shared phenotype from each that motility was significantly reduced...
2017: PloS One
https://www.readbyqxmd.com/read/28678757/the-therapeutic-potential-of-cell-cycle-targeting-in-multiple-myeloma
#14
REVIEW
Anke Maes, Eline Menu, Kim De Veirman, Ken Maes, Karin Vanderkerken, Elke De Bruyne
Proper cell cycle progression through the interphase and mitosis is regulated by coordinated activation of important cell cycle proteins (including cyclin-dependent kinases and mitotic kinases) and several checkpoint pathways. Aberrant activity of these cell cycle proteins and checkpoint pathways results in deregulation of cell cycle progression, which is one of the key hallmarks of cancer. Consequently, intensive research on targeting these cell cycle regulatory proteins identified several candidate small molecule inhibitors that are able to induce cell cycle arrest and even apoptosis in cancer cells...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28676645/kif5a-transports-collagen-vesicles-of-myofibroblasts-during-pleural-fibrosis
#15
Hirotoshi Kamata, Yoshikazu Tsukasaki, Tsuyoshi Sakai, Reiko Ikebe, Julia Wang, Ann Jeffers, Jake Boren, Shuzi Owens, Takahiro Suzuki, Masaaki Higashihara, Steven Idell, Torry A Tucker, Mitsuo Ikebe
Fibrosis involves the production of extracellular matrix proteins in tissues and is often preceded by injury or trauma. In pleural fibrosis excess collagen deposition results in pleural thickening, increased stiffness and impaired lung function. Myofibroblasts are responsible for increased collagen deposition, however the molecular mechanism of transportation of procollagen containing vesicles for secretion is unknown. Here, we studied the role of kinesin on collagen-1 (Col-1) containing vesicle transportation in human pleural mesothelial cells (HPMCs)...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28673988/four-stranded-mini-microtubules-formed-by-prosthecobacter-btubab-show-dynamic-instability
#16
Xian Deng, Gero Fink, Tanmay A M Bharat, Shaoda He, Danguole Kureisaite-Ciziene, Jan Löwe
Microtubules, the dynamic, yet stiff hollow tubes built from αβ-tubulin protein heterodimers, are thought to be present only in eukaryotic cells. Here, we report a 3.6-Å helical reconstruction electron cryomicroscopy structure of four-stranded mini microtubules formed by bacterial tubulin-like Prosthecobacter dejongeii BtubAB proteins. Despite their much smaller diameter, mini microtubules share many key structural features with eukaryotic microtubules, such as an M-loop, alternating subunits, and a seam that breaks overall helical symmetry...
July 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28671279/impairment-of-primary-cilia-contributes-to-visceral-adiposity-of-high-fat-diet-fed-mice
#17
Ni Qiu, Wei-Jin Fang, Hong-Sheng Li, Zhi-Min He, Zhou-Sheng Xiao, Yan Xiong
Deficiency of primary cilia formation by knockout kinesin family member 3A (Kif3a) in mature osteoblasts led to osteopenia and enhanced adipogenesis. Adipogenesis plays an important role in adipose tissue expansion by High-fat-diet (HFD) induced obesity. Whether primary cilia participate in high-fat-diet induced adiposity remains unclear. In this study, we found that the number and length of primary cilia and expression levels of KIF3A and intraflagellar transport 88 homolog (IFT88) mRNA and proteins reached peak on the day 3 of adipogenesis, followed by a decrease to reach low basal expression levels at day 9 when differentiated to lipid accumulating adipocytes in VAT-SVFs derived from lean mice...
July 3, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28668932/molecular-mechanisms-of-kinesin-14-motors-in-spindle-assembly-and-chromosome-segregation
#18
REVIEW
Zhen-Yu She, Wan-Xi Yang
During eukaryote cell division, molecular motors are crucial regulators of microtubule organization, spindle assembly, chromosome segregation and intracellular transport. The kinesin-14 motors are evolutionarily conserved minus-end-directed kinesin motors that occur in diverse organisms from simple yeasts to higher eukaryotes. Members of the kinesin-14 motor family can bind to, crosslink or slide microtubules and, thus, regulate microtubule organization and spindle assembly. In this Commentary, we present the common subthemes that have emerged from studies of the molecular kinetics and mechanics of kinesin-14 motors, particularly with regard to their non-processive movement, their ability to crosslink microtubules and interact with the minus- and plus-ends of microtubules, and with microtubule-organizing center proteins...
July 1, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28663865/multimodal-nonlinear-optical-microscopy-reveals-critical-role-of-kinesin-1-in-cartilage-development
#19
Sicong He, Wenqian Xue, Zhigang Duan, Qiqi Sun, Xuesong Li, Huiyan Gan, Jiandong Huang, Jianan Y Qu
We developed a multimodal nonlinear optical (NLO) microscope system by integrating stimulated Raman scattering (SRS), second harmonic generation (SHG) and two-photon excited fluorescence (TPEF) imaging. The system was used to study the morphological and biochemical characteristics of tibial cartilage in a kinesin-1 (Kif5b) knockout mouse model. The detailed structure of fibrillar collagen in the extracellular matrix of cartilage was visualized by the forward and backward SHG signals, while high resolution imaging of chondrocytes was achieved by capturing endogenous TPEF and SRS signals of the cells...
March 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28655204/trak2-a-novel-regulator-of-abca1-expression-cholesterol-efflux-and-hdl-biogenesis
#20
Nicole J Lake, Rachael L Taylor, Hugh Trahair, K N Harikrishnan, Joanne E Curran, Marcio Almeida, Hemant Kulkarni, Nigora Mukhamedova, Anh Hoang, Hann Low, Andrew J Murphy, Matthew P Johnson, Thomas D Dyer, Michael C Mahaney, Harald H H Göring, Eric K Moses, Dmitri Sviridov, John Blangero, Jeremy B M Jowett, Kiymet Bozaoglu
Aims: The recent failures of HDL-raising therapies have underscored our incomplete understanding of HDL biology. Therefore there is an urgent need to comprehensively investigate HDL metabolism to enable the development of effective HDL-centric therapies. To identify novel regulators of HDL metabolism, we performed a joint analysis of human genetic, transcriptomic, and plasma HDL-cholesterol (HDL-C) concentration data and identified a novel association between trafficking protein, kinesin binding 2 (TRAK2) and HDL-C concentration...
June 26, 2017: European Heart Journal
keyword
keyword
41642
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"