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https://www.readbyqxmd.com/read/29668079/co-culture-of-adipose-derived-stem-cells-and-chondrocytes-on-three-dimensionally-printed-bioscaffolds-for-craniofacial-cartilage-engineering
#1
Robert J Morrison, Hassan B Nasser, Khaled N Kashlan, David A Zopf, Derek J Milner, Colleen L Flanangan, Matthew B Wheeler, Glenn E Green, Scott J Hollister
OBJECTIVES/HYPOTHESIS: Reconstruction of craniofacial cartilagenous defects are among the most challenging surgical procedures in facial plastic surgery. Bioengineered craniofacial cartilage holds immense potential to surpass current reconstructive options, but limitations to clinical translation exist. We endeavored to determine the viability of utilizing adipose-derived stem cell-chondrocyte co-culture and three-dimensional (3D) printing to produce 3D bioscaffolds for cartilage tissue engineering...
April 18, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29667491/optimization-of-3d-print-material-for-the-recreation-of-patient-specific-temporal-bone-models
#2
Max Haffner, Austin Quinn, Tsung-Yen Hsieh, E Bradley Strong, Toby Steele
OBJECTIVE: Identify the 3D printed material that most accurately recreates the visual, tactile, and kinesthetic properties of human temporal bone Subjects and Methods: Fifteen study participants with an average of 3.6 years of postgraduate training and 56.5 temporal bone (TB) procedures participated. Each participant performed a mastoidectomy on human cadaveric TB and five 3D printed TBs of different materials. After drilling each unique material, participants completed surveys to assess each model's appearance and physical likeness on a Likert scale from 0 to 10 (0 = poorly representative, 10 = completely life-like)...
May 2018: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/29666857/chitosan-catechol-a-writable-bioink-under-serum-culture-media
#3
Daiheon Lee, Joseph P Park, Mi-Young Koh, Pureum Kim, Junhee Lee, Mikyung Shin, Haeshin Lee
Mussel-inspired adhesive coatings on biomedical devices have attracted significant interest due to their unique properties such as substrate independency and high efficiency. The key molecules for mussel-inspired adhesive coatings are catechol and amine groups. Along with the understanding of catechol chemistry, chitosan-catechol has also been developed as a representative mussel-inpired adhesive polymer that contains catechol and amine groups for adhesiveness. Herein, we demonstrated the direct writability of chitosan-catechol as a bioink for 3D printing, one of the additive techniques...
April 18, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29666244/spatial-determinants-of-quorum-signaling-in-a-pseudomonas-aeruginosa-infection-model
#4
Sophie E Darch, Olja Simoska, Mignon Fitzpatrick, Juan P Barraza, Keith J Stevenson, Roger T Bonnecaze, Jason B Shear, Marvin Whiteley
Quorum sensing (QS) is a bacterial communication system that involves production and sensing of extracellular signals. In laboratory models, QS allows bacteria to monitor and respond to their own cell density and is critical for fitness. However, how QS proceeds in natural, spatially structured bacterial communities is not well understood, which significantly hampers our understanding of the emergent properties of natural communities. To address this gap, we assessed QS signaling in the opportunistic pathogen Pseudomonas aeruginosa in a cystic fibrosis (CF) lung infection model that recapitulates the biogeographical aspects of the natural human infection...
April 17, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29665579/correction-measurements-agreement-between-low-cost-and-high-level-handheld-3d-scanners-to-scan-the-knee-for-designing-a-3d-printed-knee-brace
#5
Yoann Dessery, Jari Pallari
[This corrects the article DOI: 10.1371/journal.pone.0190585.].
2018: PloS One
https://www.readbyqxmd.com/read/29665336/degradable-3d-printed-hydrogels-based-on-star-shaped-copolypeptides
#6
Robert Murphy, David P Walsh, Charles A Hamilton, Sally-Ann Cryan, Marc In Het Panhuis, Andreas Heise
We present a star copolypeptide based hydrogel ink capable of structural microfabrication using 3D extrusion printing. The material comprises an amphiphilic block copolymer structure of poly(benzyl-L-glutamate-b-L-valine), which spontaneously form hydrogels through hydrophobic interaction. The chemical design allows the bulk phase of the hydrogel to remain intact after application of shear due to its self-recovery behavior. It is demonstrated that the composition of the materials is ideally suited for 3D printing; with scaffolds capable of maintaining structural cohesion after extrusion...
April 17, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29665285/hemostatic-and-absorbent-polyhipe-kaolin-composites-for-3d-printable-wound-dressing-materials
#7
Benjamin C Streifel, Jeffrey G Lundin, Allix M Sanders, Karli A Gold, Thomas S Wilems, Sierra J Williams, Elizabeth Cosgriff-Hernandez, James H Wynne
A novel hemostatic and absorbent wound dressing material compatible with 3D printing is developed to address deficiencies in current wound dressing protocol. The design involves an open celled, microporous hydrogel foam via a high internal phase emulsion (HIPE) template with biocompatible components and tunable hemostatic character by kaolin loading, the viscosity and cure kinetics of which are tailored for 3D printing applications. The use of nontoxic mineral oil organic phase results in cytocompatability with human dermal fibroblasts...
April 17, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29663706/patient-specific-bioinks-for-3d-bioprinting-of-tissue-engineering-scaffolds
#8
Negar Faramarzi, Iman K Yazdi, Mahboubeh Nabavinia, Andrea Gemma, Adele Fanelli, Andrea Caizzone, Leon M Ptaszek, Indranil Sinha, Ali Khademhosseini, Jeremy N Ruskin, Ali Tamayol
Bioprinting has emerged as a promising tool in tissue engineering and regenerative medicine. Various 3D printing strategies have been developed to enable bioprinting of various biopolymers and hydrogels. However, the incorporation of biological factors has not been well explored. As the importance of personalized medicine is becoming more clear, the need for the development of bioinks containing autologous/patient-specific biological factors for tissue engineering applications becomes more evident. Platelet-rich plasma (PRP) is used as a patient-specific source of autologous growth factors that can be easily incorporated to hydrogels and printed into 3D constructs...
April 16, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29663272/fiducial-based-fusion-of-3d-dental-models-with-magnetic-resonance-imaging
#9
Amir H Abdi, Alan G Hannam, Sidney Fels
PURPOSE: Magnetic resonance imaging (MRI) is widely used in study of maxillofacial structures. While MRI is the modality of choice for soft tissues, it fails to capture hard tissues such as bone and teeth. Virtual dental models, acquired by optical 3D scanners, are becoming more accessible for dental practice and are starting to replace the conventional dental impressions. The goal of this research is to fuse the high-resolution 3D dental models with MRI to enhance the value of imaging for applications where detailed analysis of maxillofacial structures are needed such as patient examination, surgical planning, and modeling...
April 16, 2018: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/29661300/chitosan-functionalized-poly-%C3%AE%C2%B5-caprolactone-electrospun-fibers-and-3d-printed-scaffolds-as-antibacterial-materials-for-tissue-engineering-applications
#10
Myriam G Tardajos, Giuseppe Cama, Mamoni Dash, Lara Misseeuw, Tom Gheysens, Christian Gorzelanny, Tom Coenye, Peter Dubruel
Tissue engineering (TE) approaches often employ polymer-based scaffolds to provide support with a view to the improved regeneration of damaged tissues. The aim of this research was to develop a surface modification method for introducing chitosan as an antibacterial agent in both electrospun membranes and 3D printed poly-ε-caprolactone (PCL) scaffolds. The scaffolds were functionalized by grafting methacrylic acid N-hydroxysuccinimide ester (NHSMA) onto the surface after Ar-plasma/air activation. Subsequently, the newly-introduced NHS groups were used to couple with chitosan of various molecular weights (Mw)...
July 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29661210/use-of-a-life-size-three-dimensional-printed-spine-model-for-pedicle-screw-instrumentation-training
#11
Hyun Jin Park, Chenyu Wang, Kyung Ho Choi, Hyong Nyun Kim
BACKGROUND: Training beginners of the pedicle screw instrumentation technique in the operating room is limited because of issues related to patient safety and surgical efficiency. Three-dimensional (3D) printing enables training or simulation surgery on a real-size replica of deformed spine, which is difficult to perform in the usual cadaver or surrogate plastic models. The purpose of this study was to evaluate the educational effect of using a real-size 3D-printed spine model for training beginners of the free-hand pedicle screw instrumentation technique...
April 16, 2018: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29660548/how-to-deal-with-the-empty-space-after-organ-removal-for-transplantation-a-single-medical-center-experience
#12
Ding-Ping Sun, Ling-Hsien Lee, Yu-Feng Tian, Hong-Xiang Zheng, Jinn-Rung Kuo, Che-Chuan Wang
PURPOSE: Dealing with the empty space after organ removal for transplantation has not been investigated. MATERIALS AND METHODS: From January 28, 2005, to November 21, 2017, 111 organ donors were enrolled in this study. They were divided into three groups: no replacement, replaced with paper printed with organ graphics, or replaced with 3D-printed simulated organs after the organs were removed at different periods. The donor's age, gender, etiology of admission, characteristic and clinical pictures, time interval between admission and the date of donation, and time interval between donor coordinator consultations were evaluated...
April 13, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29660055/accuracy-assessment-of-pedicle-and-lateral-mass-screw-insertion-assisted-by-customized-3d-printed-drill-guides-a-human-cadaver-study
#13
Peter A J Pijpker, Joep Kraeima, Max J H Witjes, D L Marinus Oterdoom, Maarten H Coppes, Rob J M Groen, Jos M A Kuijlen
BACKGROUND: Accurate cervical screw insertion is of paramount importance considering the risk of damage to adjacent vital structures. Recent research in 3-dimensional (3D) technology describes the advantage of patient-specific drill guides for accurate screw positioning, but consensus about the optimal guide design and the accuracy is lacking. OBJECTIVE: To find the optimal design and to evaluate the accuracy of individualized 3D-printed drill guides for lateral mass and pedicle screw placement in the cervical and upper thoracic spine...
April 5, 2018: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/29659132/3d-extrusion-printing-induces-polymer-molecule-alignment-and-cell-organization-within-engineered-cartilage
#14
Ting Guo, Julia P Ringel, Casey G Lim, Laura G Bracaglia, Maeesha Noshin, Hannah B Baker, Douglas A Powell, John P Fisher
Proper cell-material interactions are critical to remain cell function and thus successful tissue regeneration. Many fabrication processes have been developed to create microenvironments to control cell attachment and organization on a 3D scaffold. However, these approaches often involve heavy engineering and only the surface layer can be patterned. We found that 3D extrusion based printing at high temperature and pressure will result an aligned effect on the polymer molecules, and this molecular arrangement will further induce the cell alignment and different differentiation capacities...
April 16, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29658935/fabrication-of-custom-agarose-wells-for-cell-seeding-and-tissue-ring-self-assembly-using-3d-printed-molds
#15
Hannah A Strobel, Elizabeth L Calamari, Brittany Alphonse, Tracy A Hookway, Marsha W Rolle
Engineered tissues are being used clinically for tissue repair and replacement, and are being developed as tools for drug screening and human disease modeling. Self-assembled tissues offer advantages over scaffold-based tissue engineering, such as enhanced matrix deposition, strength, and function. However, there are few available methods for fabricating 3D tissues without seeding cells on or within a supporting scaffold. Previously, we developed a system for fabricating self-assembled tissue rings by seeding cells into non-adhesive agarose wells...
April 2, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29658700/3d-printed-graphene-polylactic-acid-electrodes-promise-high-sensitivity-in-electroanalysis
#16
C Lorena Manzanares Palenzuela, Filip Novotný, Petr Krupička, Zdeněk Sofer, Martin Pumera
Additive manufacturing provides a unique tool for prototyping structures toward electrochemical sensing, due to its ability to produce highly versatile, tailored-shaped devices in a low-cost and fast way with minimized waste. Here we present 3D-printed graphene electrodes for electrochemical sensing. Ring- and disc-shaped electrodes were 3D-printed with a Fused Deposition Modeling printer and characterized using cyclic voltammetry and scanning electron microscopy. Different redox probes K3 Fe(CN)6 :K4 Fe(CN)6 , FeCl3 , ascorbic acid, Ru(NH3 )6 Cl3 , and ferrocene monocarboxylic acid) were used to assess the electrochemical performance of these devices...
April 16, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29658697/enhanced-impact-resistance-of-3d-printed-parts-with-structured-filaments
#17
Fang Peng, Zhiyang Zhao, Xuhui Xia, Mukerrem Miko Cakmak, Bryan D Vogt
Net shape manufacture of customizable objects through 3D printing offers tremendous promise for personalization to improve the fit, performance and comfort associated with devices and tools used in our daily lives. However, the application of 3D printing in structural objects is limited by their poor mechanical performance that manifests from the layer-by-layer process by which the part is produced. Here, this interfacial weakness is overcome by a structured, core-shell polymer filament where a polycarbonate (PC) core solidifies quickly to define the shape, while an olefin ionomer shell contains functionality (crystallinity and ionic) that strengthen the interface between the printed layers...
April 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29658053/flow-and-air-conditioning-simulations-of-computer-turbinectomized-nose-models
#18
J Pérez-Mota, F Solorio-Ordaz, J Cervantes-de Gortari
Air conditioning for the human respiratory system is the most important function of the nose. When obstruction occurs in the nasal airway, turbinectomy is used to correct such pathology. However, mucosal atrophy may occur sometime after this surgery when it is overdone. There is not enough information about long-term recovery of nasal air conditioning performance after partial or total surgery. The purpose of this research was to assess if, based on the flow and temperature/humidity characteristics of the air intake to the choana, partial resection of turbinates is better than total resection...
April 16, 2018: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/29656568/nanofiber-based-hydrogels-controllable-synthesis-and-multifunctional-applications
#19
REVIEW
Qiuxia Fu, Cheng Duan, Zishuo Yan, Yan Li, Yang Si, Lifang Liu, Jianyong Yu, Bin Ding
Nanofiber-based hydrogels (NFHGs) prepared by the combination of traditional hydrogels and novel nanofibers have demonstrated great potential in various application fields, owing to their integrated advantages of superhydrophilicity, high water-holding capacity, good biocompatibility, enhanced mechanical strength, and excellent structural tenability. In this review, a comprehensive overview of the structure design and synthetic strategy of NFHGs derived from electrospinning technique, weaving, freeze-drying, 3D printing, and molecular self-assembling method is provided...
April 14, 2018: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/29656556/melt-electrowriting-of-thermoplastic-elastomers
#20
Gernot Hochleitner, Eva Fürsattel, Reiner Giesa, Jürgen Groll, Hans-Werner Schmidt, Paul D Dalton
Melt electrowriting (MEW), an additive manufacturing process, is established using polycaprolactone as the benchmark material. In this study, a thermoplastic elastomer, namely, poly(urea-siloxane), is synthesized and characterized to identify how different classes of polymers are compatible with MEW. This polyaddition polymer has reversible hydrogen bonding from the melt upon heating/cooling and highly resolved structures are achieved by MEW. The influence of applied voltage, temperature, and feeding pressure on printing outcomes behavior is optimized...
April 14, 2018: Macromolecular Rapid Communications
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