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https://www.readbyqxmd.com/read/28386673/development-of-an-agrobacterium-mediated-transformation-method-and-evaluation-of-two-exogenous-constitutive-promoters-in-oleaginous-yeast-lipomyces-starkeyi
#1
Xinping Lin, Sasa Liu, Ruiqi Bao, Ning Gao, Sufang Zhang, Rongqian Zhu, Zongbao Kent Zhao
Oleaginous yeast Lipomyces starkeyi, a promising strain of great biotechnical importance, is able to accumulate over 60% of its cell biomass as triacylglycerols (TAGs). It is promising to directly produce the derivatives of TAGs, such as long-chain fatty acid methyl esters and alkanes, in L. starkeyi. However, techniques for genetic modification of this oleaginous yeast are lacking, thus, further research is needed to develop genetic tools and functional elements. Here, we used two exogenous promoters (pGPD and pPGK) from oleaginous yeast Rhodosporidium toruloides to establish a simpler Agrobacterium-mediated transformation (AMT) method for L...
April 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28364635/hydrothermal-treatment-followed-by-enzymatic-hydrolysis-and-hydrothermal-carbonization-as-means-to-valorise-agro-and-forest-based-biomass-residues
#2
Hanne Wikberg, Stina Grönqvist, Piritta Niemi, Atte Mikkelson, Matti Siika-Aho, Heimo Kanerva, Andres Käsper, Tarja Tamminen
The suitability of several abundant but underutilized agro and forest based biomass residues for hydrothermal treatment followed by enzymatic hydrolysis as well as for hydrothermal carbonization was studied. The selected approaches represent simple biotechnical and thermochemical treatment routes suitable for wet biomass. Based on the results, the hydrothermal pre-treatment followed by enzymatic hydrolysis seemed to be most suitable for processing of carbohydrate rich corn leaves, corn stover, wheat straw and willow...
March 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28336852/amorphous-silica-particles-relevant-in-food-industry-influence-cellular-growth-and-associated-signaling-pathways-in-human-gastric-carcinoma-cells
#3
Anja Wittig, Helge Gehrke, Giorgia Del Favero, Eva-Maria Fritz, Marco Al-Rawi, Silvia Diabaté, Carsten Weiss, Haider Sami, Manfred Ogris, Doris Marko
Nanostructured silica particles are commonly used in biomedical and biotechnical fields, as well as, in cosmetics and food industry. Thus, their environmental and health impacts are of great interest and effects after oral uptake are only rarely investigated. In the present study, the toxicological effects of commercially available nano-scaled silica with a nominal primary diameter of 12 nm were investigated on the human gastric carcinoma cell line GXF251L. Besides the analysis of cytotoxic and proliferative effects and the comparison with effects of particles with a nominal primary diameter of 200 nm, emphasis was also given to their influence on the cellular epidermal growth factor receptor (EGFR) and mitogen-activated protein kinases (MAPK) signaling pathways-both of them deeply involved in the regulation of cellular processes like cell cycle progression, differentiation or proliferation...
January 13, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28332831/layer-by-layer-assembly-of-amine-reactive-multilayers-using-an-azlactone-functionalized-polymer-and-small-molecule-diamine-linkers
#4
Yashira M Zayas-Gonzalez, Benjamín J Ortiz, David M Lynn
We report the reactive layer-by-layer assembly of amine-reactive polymer multilayers using an azlactone-functionalized polymer and small-molecule diamine linkers. This approach yields cross-linked polymer/linker-type films that can be further functionalized, after fabrication, by treatment with functional primary amines, and provides opportunities to incorporate other useful functionality that can be difficult to introduce using other polyamine building blocks. Films fabricated using poly(2-vinyl-4,4-dimethylazlactone) (PVDMA) and three model nondegradable aliphatic diamine linkers yielded reactive thin films that were stable upon incubation in physiologically relevant media...
April 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28330071/unexpected-formation-of-n-phenyl-thiophosphorohydrazidic-acid-o-s-dimethyl-ester-from-acephate-chemical-biotechnical-and-computational-study
#5
Vijay Kumar, Sukhmanpreet Kaur, Simranjeet Singh, Niraj Upadhyay
By the nucleophilic attack of phenylhydrazine on acephate in aqueous medium, a stable product N'-phenyl-thiophosphorohydrazidic acid O,S-dimethyl ester (1) was obtained and characterized by elemental, spectral and thermal analysis. Thermodynamic parameters, Ea, ΔH°, ΔS° and ΔG°, have found to be 62.15, -67.95, -0.068 and -20.05 kJ mol(-1) according to the Coats-Redfern equation. Analysis of interaction of 1 with BSA protein was done by using the UV-Vis and FTIR spectroscopic methods. The observed binding constants was 1...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28323242/investigation-of-the-indigenous-fungal-community-populating-barley-grains-secretomes-and-xylanolytic-potential
#6
Abida Sultan, Jens C Frisvad, Birgit Andersen, Birte Svensson, Christine Finnie
The indigenous fungal species populating cereal grains produce numerous plant cell wall-degrading enzymes including xylanases, which could play important role in plant-pathogen interactions and in adaptation of the fungi to varying carbon sources. To gain more insight into the grain surface-associated enzyme activity, members of the populating fungal community were isolated, and their secretomes and xylanolytic activities assessed. Twenty-seven different fungal species were isolated from grains of six barley cultivars over different harvest years and growing sites...
March 18, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28290585/size-and-property-bimodality-in-magnetic-nanoparticle-dispersions-single-domain-particles-vs-strongly-coupled-nanoclusters
#7
E Wetterskog, A Castro, L Zeng, S Petronis, D Heinke, E Olsson, L Nilsson, N Gehrke, P Svedlindh
The widespread use of magnetic nanoparticles in the biotechnical sector puts new demands on fast and quantitative characterization techniques for nanoparticle dispersions. In this work, we report the use of asymmetric flow field-flow fractionation (AF4) and ferromagnetic resonance (FMR) to study the properties of a commercial magnetic nanoparticle dispersion. We demonstrate the effectiveness of both techniques when subjected to a dispersion with a bimodal size/magnetic property distribution: i.e., a small superparamagnetic fraction, and a larger blocked fraction of strongly coupled colloidal nanoclusters...
March 14, 2017: Nanoscale
https://www.readbyqxmd.com/read/28219288/the-human-figure-and-urban-ground-cyborgs-and-the-city
#8
Aaron Parkhurst
Recent years have seen developments in cyborgian movements in which people play and experiment with the boundaries of human flesh and aesthetic realities. Some individuals develop novel intimate relationships with devices that, each in their own way, interfere with human subjectivity. Public dissemination of these movements often proposes notions of transcendence, therapy, post-humanism and trans-humanism, but often fails to outline the lived-experience of those who explore cyborgian identity as an alternative mode of being in the world...
July 2016: New Bioethics: a Multidisciplinary Journal of Biotechnology and the Body
https://www.readbyqxmd.com/read/28188331/urnula-sp-an-endophyte-of-dicksonia-antarctica-making-a-fragrant-mixture-of-biologically-active-volatile-organic-compounds
#9
Gary Strobel, Amy Ericksen, Joe Sears, Jie Xie, Brad Geary, Bryan Blatt
Urnula sp. was isolated as an endophyte of Dicksonia antarctica and identified primarily on the basis of its ITS sequence and morphological features. The anamorphic state of the fungus appeared as a hyphomyceteous-like fungus as based on its features in culture and scanning electron microscopy examination of its spores. On potato dextrose agar (PDA), the organism makes a characteristic fragrance resembling peach pie with vanilla overtones. A GC/MS analysis done on the volatile organic compounds (VOCs) of this organism, trapped by carbotrap methodology, revealed over 150 compounds with high MS matching quality being noted for 44 of these...
February 10, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28161570/safety-of-a-killed-oral-cholera-vaccine-shanchol-in-pregnant-women-in-malawi-an-observational-cohort-study
#10
Mohammad Ali, Allyson Nelson, Francisco J Luquero, Andrew S Azman, Amanda K Debes, Maurice Mwesawina M'bang'ombe, Linly Seyama, Evans Kachale, Kingsley Zuze, Desire Malichi, Fatima Zulu, Kelias Phiri Msyamboza, Storn Kabuluzi, David A Sack
BACKGROUND: Pregnancy increases the risk of harmful effects from cholera for both mothers and their fetuses. A killed oral cholera vaccine, Shanchol (Shantha Biotechnics, Hydrabad, India), can protect against the disease for up to 5 years. However, cholera vaccination campaigns have often excluded pregnant women because of insufficient safety data for use during pregnancy. We did an observational cohort study to assess the safety of Shanchol during pregnancy. METHODS: This observational cohort study was done in two adjacent districts (Nsanje and Chikwawa) in Malawi...
May 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28144899/problematising-the-discourse-of-post-aids
#11
Liz Walker
This paper reflects on the meanings of 'post-AIDS' in the Global North and South. I bring together contemporary arguments to suggest that the notion of 'post-AIDS' is, at best, misplaced, not least because its starting point remains a biotechnical one. Drawing on aspects of the sub-Saharan African experience, this essay suggests that, despite significant shifts in access to antiretroviral therapy (ART), HIV continues to be fundamentally shaped by economic determinants and social and cultural practices. In this essay, I question the certainty of the discourse of (Western biomedical) 'positive progress' (Johnson et al...
February 1, 2017: Journal of Medical Humanities
https://www.readbyqxmd.com/read/27917290/functional-analysis-of-alternative-splicing-of-the-flowering-locus-t-orthologous-gene-in-chrysanthemum-morifolium
#12
Yachao Mao, Jing Sun, Peipei Cao, Rong Zhang, Qike Fu, Sumei Chen, Fadi Chen, Jiafu Jiang
As the junction of floral development pathways, the FLOWERING LOCUS T (FT) protein called 'florigen' plays an important role in the process of plant flowering through signal integration. We isolated four transcripts encoding different isoforms of a FT orthologous gene CmFTL1, from Chrysanthemum morifolium cultivar 'Jimba'. Sequence alignments suggested that the four transcripts are related to the intron 1. Expression analysis showed that four alternative splicing (AS) forms of CmFTL1 varied depending on the developmental stage of the flower...
2016: Horticulture Research
https://www.readbyqxmd.com/read/27890022/continuous-light-after-2-months-of-long-days-stimulates-ram-testis-volume-and-increases-fertility-in-spring
#13
D Chesneau, D Guillaume, P Chemineau, B Malpaux
Seasonal reproduction is one of the major biotechnical and economic constraints of sheep production in temperate latitudes. Treatments using extra light followed by melatonin implants have been used satisfactorily in open barns, farms and artificial insemination centres to produce out-of-season sexual activity in rams. The aim of the present study is to explore the possibility of replacing melatonin implants with continuous light (LL), which was recently shown to increase LH secretion similar to melatonin and/or pinealectomy...
November 28, 2016: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/27849391/reviewers-for-biotechnic-histochemistry-2015-2016
#14
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/27765293/effectiveness-of-one-dose-of-oral-cholera-vaccine-in-response-to-an-outbreak-a-case-cohort-study
#15
Andrew S Azman, Lucy A Parker, John Rumunu, Fisseha Tadesse, Francesco Grandesso, Lul L Deng, Richard Laku Lino, Bior K Bior, Michael Lasuba, Anne-Laure Page, Lameck Ontweka, Augusto E Llosa, Sandra Cohuet, Lorenzo Pezzoli, Dossou Vincent Sodjinou, Abdinasir Abubakar, Amanda K Debes, Allan M Mpairwe, Joseph F Wamala, Christine Jamet, Justin Lessler, David A Sack, Marie-Laure Quilici, Iza Ciglenecki, Francisco J Luquero
BACKGROUND: Oral cholera vaccines represent a new effective tool to fight cholera and are licensed as two-dose regimens with 2-4 weeks between doses. Evidence from previous studies suggests that a single dose of oral cholera vaccine might provide substantial direct protection against cholera. During a cholera outbreak in May, 2015, in Juba, South Sudan, the Ministry of Health, Médecins Sans Frontières, and partners engaged in the first field deployment of a single dose of oral cholera vaccine to enhance the outbreak response...
November 2016: Lancet Global Health
https://www.readbyqxmd.com/read/27602704/influence-of-gestational-age-secretor-and-lewis-blood-group-status-on-the-oligosaccharide-content-of-human-milk
#16
Clemens Kunz, Christina Meyer, Maria Carmen Collado, Lena Geiger, Izaskun García-Mantrana, Bibiana Bertua-Ríos, Cecilia Martínez-Costa, Christian Borsch, Silvia Rudloff
OBJECTIVES: Human milk oligosaccharides (HMOs) are considered to play an important role for the infant. As the biotechnical production of some HMOs is feasible today and clinical studies are being designed, the individual variation of the total amount of HMOs and of single components is of particular importance. Our objectives were to investigate whether differences exist between term and preterm milk, milk from mothers with secretor or nonsecretor status, and a Lewis blood group (a+b-), (a-b+), or (a-b-) pattern...
May 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/27508537/rotavirus-specific-salivary-and-fecal-iga-in-indian-children-and-adults
#17
Anu Paul, Sudhir Babji, Rajiv Sarkar, Robin Penugula Lazarus, Gagandeep Kang
OBJECTIVE: To compare serum, salivary and fecal IgA responses in infants and adults following rotavirus vaccination. STUDY DESIGN: Laboratory testing of samples from clinical trials. SETTING: Medical College Hospital. PARTICIPANTS: 13 healthy adult volunteers not given vaccine, 20 healthy adult volunteers given one dose of bovine rotavirus tetravalent vaccine (Shantha Biotechnics), and 88 infants given 3 or 5 doses of Rotarix...
July 8, 2016: Indian Pediatrics
https://www.readbyqxmd.com/read/27478496/conservation-and-sustainable-use-of-medicinal-plants-problems-progress-and-prospects
#18
Shi-Lin Chen, Hua Yu, Hong-Mei Luo, Qiong Wu, Chun-Fang Li, André Steinmetz
Medicinal plants are globally valuable sources of herbal products, and they are disappearing at a high speed. This article reviews global trends, developments and prospects for the strategies and methodologies concerning the conservation and sustainable use of medicinal plant resources to provide a reliable reference for the conservation and sustainable use of medicinal plants. We emphasized that both conservation strategies (e.g. in situ and ex situ conservation and cultivation practices) and resource management (e...
2016: Chinese Medicine
https://www.readbyqxmd.com/read/27392774/chitosan-nanofiber-production-from-drosophila-by-electrospinning
#19
Murat Kaya, Bahar Akyuz, Esra Bulut, Idris Sargin, Fatma Eroglu, Gamze Tan
Drosophila melanogaster is one of the important test organisms in genetics thanks to its fast growth rate in a culture. This study demonstrates that the fly D. melanogaster can also be exploited as a source for nanofiber production in biotechnical applications. First, its chitin content was determined (7.85%) and then high molecular weight chitosan (141.4kDa) was synthesized through deacetylation of chitin isolates. Chitosan nanofibers with the diameter of 40.0073±12.347nm were produced by electrospinning of Drosophila chitosan...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27336672/low-ph-enhances-the-action-of-maximin-h5-against-staphylococcus-aureus-and-helps-mediate-lysylated-phosphatidylglycerol-induced-resistance
#20
Sarah R Dennison, Leslie Hg Morton, Frederick Harris, David A Phoenix
Maximin H5 (MH5) is an amphibian antimicrobial peptide specifically targeting Staphylococcus aureus. At pH 6, the peptide showed an improved ability to penetrate (ΔΠ = 6.2 mN m(-1)) and lyse (lysis = 48%) Staphylococcus aureus membrane mimics, which incorporated physiological levels of lysylated phosphatidylglycerol (Lys-PG, 60%), compared to that at pH 7 (ΔΠ = 5.6 mN m(-1) and lysis = 40% at pH 7) where levels of Lys-PG are lower (40%). The peptide therefore appears to have optimal function at pH levels known to be optimal for the organism's growth...
July 12, 2016: Biochemistry
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