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https://www.readbyqxmd.com/read/29025287/-incidence-and-treatment-of-extranodal-natural-killer-t-cell-lymphoma-nasal-type-hungarian-experiences
#1
Annamária Bakos, Árpád Szomor, Tamás Schneider, Zsófia Miltényi, Imelda Marton, Zita Borbényi, Judit Pammer, László Krenács, Enikő Bagdi, Klára Piukovics
INTRODUCTION: Extranodal natural killer/T (NK/T) cell lymphoma, nasal type (ENKTL) represents a rare subtype of T-cell lymphomas with aggressive clinical behavior according to WHO 2016 classification. AIM: ENKTL has distinctive geographic distribution with higher incidence in Asia and Latin America (10% of all non-Hodgkin lymphoma cases), than in Europe and North America (<1%). ENKTL tipically origins from nasopharynx and upper aerodigestive tract. Anthracycline-based chemotherapy regimens are largely ineffective in the treatment of ENKTL...
October 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/29024352/characterization-of-conjugation-pattern-in-large-polysaccharide-protein-conjugates-by-nmr
#2
Stefano Giuntini, Evita Balducci, Linda Cerofolini, Enrico Ravera, Marco Fragai, Francesco Berti, Claudio Luchinat
Carbohydrate-based vaccines are among the safest and most effective vaccines and represent potent tools for prevention of life-threatening bacterial infectious diseases, like meningitis and pneumonia. The chemical conjugation of a weak antigen to protein as a source of T-cell epitopes generates a glycoconjugate vaccine, that results more immunogenic. Several methods have been used so far to characterize the resulting polysaccharide-protein conjugates. However, a reduced number of methodologies has been proposed for measuring the degree of saccharide conjugation at the possible protein sites...
October 10, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29018079/setd2-alterations-impair-dna-damage-recognition-and-lead-to-resistance-to-chemotherapy-in-leukemia
#3
Brenton G Mar, S Haihua Chu, Josephine D Kahn, Andrei V Krivstov, Richard Koche, Cecilia A Castellano, Jacob L Kotlier, Rebecca L Zon, Marie E McConkey, Jonathan Chabon, Ryan Chappell, Peter V Grauman, James J Hsieh, Scott A Armstrong, Benjamin L Ebert
Mutations in SETD2, encoding the histone 3 lysine 36 trimethyltransferase, are enriched in relapsed acute lymphoblastic leukemia and MLL rearranged acute leukemia. We investigated the impact of SETD2 mutations on chemotherapy sensitivity in isogenic leukemia cell lines and in murine leukemia generated from a conditional knockout of Setd2SETD2 mutations led to resistance to DNA-damaging agents, cytarabine, 6-thioguanine, doxorubicin, and etoposide, but not to a non-DNA damaging agent, L-asparaginase. H3K36me3 localizes components of the DNA damage response pathway and SETD2 mutation impaired the DNA damage response (DDR), blunting apoptosis induced by cytotoxic chemotherapy...
October 10, 2017: Blood
https://www.readbyqxmd.com/read/28988014/l-asparaginase-of-leishmania-donovani-metabolic-target-and-its-role-in-amphotericin-b-resistance
#4
Jasdeep Singh, Mohd Imran Khan, Shiv Pratap Singh Yadav, Ankit Srivastava, Kislay K Sinha, Ashish, Pradeep Das, Bishwajit Kundu
Emergence of Amphotericin B (AmB) resistant Leishmania donovani has posed major therapeutic challenge against the parasite. Consequently, combination therapy aimed at multiple molecular targets, based on proteome wise network analysis has been recommended. In this regard we had earlier identified and proposed L-asparaginase of Leishmania donovani (LdAI) as a crucial metabolic target. Here we report that both LdAI overexpressing axenic amastigote and promastigote forms of L. donovani survives better when challenged with AmB as compared to wild type strain...
September 28, 2017: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/28984046/inhibition-of-telomerase-activity-and-induction-of-apoptosis-by-rhodospirillum-rubrum-l-asparaginase-in-cancer-jurkat-cell-line-and-normal-human-cd4-%C3%A2-t-lymphocytes
#5
Dmitry D Zhdanov, Vadim S Pokrovsky, Marina V Pokrovskaya, Svetlana S Alexandrova, Mikhail A Eldarov, Dmitry V Grishin, Marsel M Basharov, Yulia A Gladilina, Olga V Podobed, Nikolai N Sokolov
Rhodospirillum rubrum L-asparaginase mutant E149R, V150P, F151T (RrA) down-regulates telomerase activity due to its ability to inhibit the expression of telomerase catalytic subunit hTERT. The aim of this study was to define the effect of short-term and long-term RrA exposure on proliferation of cancer Jurkat cell line and normal human CD4(+) T lymphocytes. RrA could inhibit telomerase activity in dose- and time-dependent manner in both Jurkat and normal CD4(+) T cells. Continuous RrA exposure of these cells resulted in shortening of telomeres followed by cell cycle inhibition, replicative senescence, and development of apoptosis...
October 5, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28982056/efficacy-and-safety-of-g-csf-low-dose-cytarabine-and-aclarubicin-in-combination-with-l-asparaginase-prednisone-in-the-treatment-of-refractory-or-relapsed-acute-lymphoblastic-leukemia
#6
Keshu Zhou, Yongping Song, Yanli Zhang, Xudong Wei, Yuewen Fu, Fengkuan Yu, Hu Zhou, Xinjian Liu, Jian Zhou, Baijun Fang
Acute lymphoblastic leukemia (ALL) patients who fail to acquire complete remission (CR) or who relapse after initial response have poor prognosis. At present there is no consensus as to the standard salvage therapy for these patients. In this study, we retrospectively evaluate safety and efficacy of a salvage regimen (CAGLP) consisting of G-CSF, low-dose cytarabine, aclarubicin, l-asparaginase and prednisone. Thirty-six patients were included with primary refractory (n=13) or relapse (n=23). The overall response rate (ORR) and CR rate were 86...
September 25, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28955645/in-silico-analysis-molecular-cloning-expression-and-characterization-of-l-asparaginase-gene-from-lactobacillus-reuteri-dsm-20016
#7
Suresh Susan Aishwarya, Sellamuthu Iyappan, Kamepali Vijaya Lakshmi, Kandathil Narayanan Rajnish
l-Asparaginase is employed in leukaemic treatment and in processing starchy foods. The in silico analysis of Lactobacillus reuteri DSM 20016 reveals the presence of an l-asparaginase gene with theoretical pI value of 4.99. 3D structure prediction was carried out and one model was selected based on the validation scores of 86.293 for ERRAT, 92.10% for VERIFY 3D and Ramachandran plot. Multiple sequence alignment of the protein sequences of l-asparaginases I and II of Escherichia coli, Erwinia chrysanthemum and Homo sapiens shows their sequence similarity...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28921081/site-saturation-mutagenesis-of-mutant-l-asparaginase-ii-signal-peptide-hydrophobic-region-for-improved-excretion-of-cyclodextrin-glucanotransferase
#8
Abbas Ismail, Rosli Md Illias
The excretion of cyclodextrin glucanotransferase (CGTase) into the culture medium offers significant advantages over cytoplasmic expression. However, the limitation of Escherichia coli is its inability to excrete high amount of CGTase outside the cells. In this study, modification of the hydrophobic region of the N1R3 signal peptide using site-saturation mutagenesis improved the excretion of CGTase. Signal peptide mutants designated M9F, V10L and A15Y enhanced the excretion of CGTase three-fold and demonstrated two-fold higher secretion rate than the wild type...
September 18, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28916825/de-novo-transcriptome-of-the-cosmopolitan-dinoflagellate-amphidinium-carterae-to-identify-enzymes-with-biotechnological-potential
#9
Chiara Lauritano, Daniele De Luca, Alberto Ferrarini, Carla Avanzato, Andrea Minio, Francesco Esposito, Adrianna Ianora
Dinoflagellates are phytoplanktonic organisms found in both freshwater and marine habitats. They are often studied because related to harmful algal blooms but they are also known to produce bioactive compounds for the treatment of human pathologies. The aim of this study was to sequence the full transcriptome of the dinoflagellate Amphidinium carterae in both nitrogen-starved and -replete culturing conditions (1) to evaluate the response to nitrogen starvation at the transcriptional level, (2) to look for possible polyketide synthases (PKSs) in the studied clone (genes that may be involved in the synthesis of bioactive compounds), (3) if present, to evaluate if nutrient starvation can influence PKS expression, (4) to look for other possible enzymes of biotechnological interest and (5) to test strain cytotoxicity on human cell lines...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28911074/beyond-first-line-non-anthracycline-based-chemotherapy-for-extranodal-nk-t-cell-lymphoma-clinical-outcome-and-current-perspectives-on-salvage-therapy-for-patients-after-first-relapse-and-progression-of-disease
#10
S H Lim, J Y Hong, S T Lim, H Hong, J Arnoud, W Zhao, D H Yoon, T Tang, J Cho, S Park, Y H Ko, S J Kim, C Suh, T Lin, W S Kim
Background: Current standard treatment, including non-anthracycline-based chemotherapy and optimal combining of radiotherapy, has dramatically improved outcomes of patients with extranodal natural killer/T-cell lymphoma (ENKTL) during the last decade. This study was conducted to investigate the clinical outcome of ENKTL patients with relapsed or progressive disease after initial current standard therapy. Patients and methods: We retrospectively reviewed patients diagnosed with ENKTL at six centers in four countries (China, France, Singapore, and South Korea) from 1997 to 2015 and analyzed 179 patients who had relapsed or progressed after initial current standard therapy...
September 1, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/28899490/a-new-ph-indicator-dye-based-method-for-rapid-and-efficient-screening-of-l-asparaginase-producing-microorganisms
#11
Kanti N Mihooliya, Jitender Nandal, Laxmi Swami, Himanshu Verma, Lipsy Chopra, Debendra K Sahoo
l-asparaginase is a pharmaceutically and industrially important enzyme as it has potential to treat different cancers and inhibit acrylamide formation in fried and baked food products. In the present study, an attempt was made to screen for new and novel l-asparaginase producers using a widely applied phenol red and bromothymol blue (BTB)(1) dye-based plate assay. Screening of four different soil samples for l-asparaginase producers resulted in the isolation of three new potential l-asparaginase producing bacteria...
December 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28884068/different-methodologies-for-sustainability-of-optimization-techniques-used-in-submerged-and-solid-state-fermentation
#12
REVIEW
Anup Ashok, Devarai Santhosh Kumar
Optimization techniques are considered as a part of nature's way of adjusting to the changes happening around it. There are different factors that establish the optimum working condition or the production of any value-added product. A model is accepted for a particular process after its sustainability has been verified on a statistical and analytical level. Optimization techniques can be divided into categories as statistical, nature inspired and artificial neural network each with its own benefits and usage in particular cases...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28870016/clinical-features-and-treatment-outcomes-of-blastic-plasmacytoid-dendritic-cell-neoplasm-a-single-center-experience-in-korea
#13
Hae Su Kim, Hee-Jin Kim, Sun-Hee Kim, Joon Young Choi, Young Hyeh Ko, Won Seog Kim, Chul Won Jung, Seok Jin Kim
BACKGROUND/AIMS: Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare hematologic malignancy that typically presents in the form of skin manifestations with or without lymph node and bone marrow involvement. Given its rarity and recent recognition as a distinct pathological entity, no standard of treatment exists for this aggressive disease and its prognosis is particularly dismal. METHODS: We retrospectively analyzed clinical features and treatment outcomes of patients who were diagnosed with BPDCN between 2000 and 2014...
September 2017: Korean Journal of Internal Medicine
https://www.readbyqxmd.com/read/28860480/discovery-of-human-like-l-asparaginases-with-potential-clinical-use-by-directed-evolution
#14
Coraline Rigouin, Hien Anh Nguyen, Amanda M Schalk, Arnon Lavie
L-asparaginase is a chemotherapy drug used to treat acute lymphoblastic leukemia (ALL). The main prerequisite for clinical efficacy of L-asparaginases is micromolar KM for asparagine to allow for complete depletion of this amino acid in the blood. Since currently approved L-asparaginases are of bacterial origin, immunogenicity is a challenge, which would be mitigated by a human enzyme. However, all human L-asparaginases have millimolar KM for asparagine. We recently identified the low KM guinea pig L-asparaginase (gpASNase1)...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28855986/treatment-of-relapsed-extranodal-natural-killer-t-cell-lymphoma-with-bortezomib-plus-fludarabine
#15
Chen Chen, Hongmin He
Extranodal NK/T cell lymphoma (ENKL) is a rare entity, associated with an aggressive clinical behavior and poor prognosis. The optimal treatment strategies, particularly for relapsed patients, have not been clearly determined. A 40-year-old female ENKL patient with orbital involvement as the first presentation was initially suspected to have an orbital pseudotumor. When the patient developed disease progression following treatment with methylprednisolone, biopsy of the orbital mass was performed. Histopathological examination of the biopsy specimen was consistent with the diagnosis of ENKL (Ann Arbor stage IE)...
October 2017: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/28851634/molecular-cloning-structural-modeling-and-production-of-recombinant-aspergillus-terreusl-asparaginase-in-escherichia-coli
#16
Hesham Saeed, Hadeer Ali, Hadeer Soudan, Amira Embaby, Amany El-Sharkawy, Aida Farag, Ahmed Hussein, Farid Ataya
l-Asparaginase (EC 3.5.1.1) is an important medical enzyme that catalysis the hydrolysis of l-asparagine to aspartic acid and ammonium. For over four decades l. asparaginase utic agent for the treatment of a variety of lymphoproliferative disorders and lymphoma such as acute lymphoblastic leukemia. In the present study A. terreus full length l. asparaginase gene, 1179bp was optimized for expression in Escherichia coli BL21 (DE3) pLysS. The full length A. terreusl. asparaginase gene encoding a protein of 376 amino acids with estimated molecular weight of 42...
August 26, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28831914/enhancement-in-biological-activity-of-l-asparginase-by-its-conjugation-on-silica-nanoparticles
#17
Dorsa Golestaneh, Jaleh Varshosaz
BACKGROUND: L-asparaginase is a drug of choice in the treatment of Hodgkin&#039;s lymphoma and acute lymphoblastic leukemia. Production of its bioconjugates can increase its half-life, stability and decrease its immunogenicity. OBJECTIVE: The aim of the present study was to immobilize this drug on silica nanoparticles by two different cross-linking agents. METHOD: The drug was conjugated to nanoparticles by two cross-linking agents; 1-ethyl-3-(3-dimethylaminopropyl) carboiimide HCl (EDC) or glutaraldehyde...
August 23, 2017: Recent Patents on Nanotechnology
https://www.readbyqxmd.com/read/28830598/do-immunoglobulin-g-and-immunoglobulin-e-anti-l-asparaginase-antibodies-have-distinct-implications-in-children-with-acute-lymphoblastic-leukemia-a-cross-sectional-study
#18
Gabriela Galindo-Rodríguez, José C Jaime-Pérez, Mario C Salinas-Carmona, Sandra N González-Díaz, Ángeles Castro-Corona, Raúl Cavazos-González, Humberto Treviño-Villarreal, Alberto C Heredia-Salazar, David Gómez-Almaguer
BACKGROUND: l-Asparaginase is essential in the treatment of childhood acute lymphoblastic leukemia. If immunoglobulin G anti-l-asparaginase antibodies develop, they can lead to faster plasma clearance and reduced efficiency as well as to hypersensitivity reactions, in which immunoglobulin E can also participate. This study investigated the presence of immunoglobulin G and immunoglobulin E anti-l-asparaginase antibodies and their clinical associations. METHODS: Under 16-year-old patients at diagnosis of B-cell acute lymphoblastic leukemia confirmed by flow cytometry and treated with a uniform l-asparaginase and chemotherapy protocol were studied...
July 2017: Revista Brasileira de Hematologia e Hemoterapia
https://www.readbyqxmd.com/read/28801829/cloning-and-characterization-of-halomonas-elongata-l-asparaginase-a-promising-chemotherapeutic-agent
#19
Ali Ghasemi, Sedigheh Asad, Mahboubeh Kabiri, Bahareh Dabirmanesh
L-asparaginase has been used in the treatment of patients with acute lymphoblastic leukemia (ALL) for more than 30 years. Rapid clearance of the enzyme from blood stream and its L-glutaminase-dependent neurotoxicity has led to searching for new L-asparaginases with more desirable properties. In the present study, L-asparaginase coding gene of Halomonas elongata was isolated, expressed in Escherichia coli, purified, and characterized. The purified protein was found to have a molecular mass of 39.5 kDa and 1000-folds more activity towards L-asparagine than L-glutamine...
August 11, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28766374/biotechnological-production-and-practical-application-of-l-asparaginase-enzyme
#20
Archana Vimal, Awanish Kumar
L-asparaginase is a vital enzyme of medical importance, and renowned as a chemotherapeutic agent. The relevance of this enzyme is not only limited as an anti-cancer agent, it also possesses a wide range of medical application. The application includes the antimicrobial property, treatment of infectious diseases, autoimmune diseases, canine and feline cancer. Apart from the health care industry, its significance is also established in the food sector as a food processing agent to reduce the acrylamide concentration...
August 2, 2017: Biotechnology & Genetic Engineering Reviews
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