keyword
MENU ▼
Read by QxMD icon Read
search

Recombination

keyword
https://www.readbyqxmd.com/read/29679865/generation-and-characterization-of-hagfish-variable-lymphocyte-receptor-b-against-glycoprotein-of-viral-hemorrhagic-septicemia-virus-vhsv
#1
Jung Seok Lee, Jaesung Kim, Se Pyeong Im, Si Won Kim, Jassy Mary S Lazarte, Jae Wook Jung, Tae Won Gong, Young Rim Kim, Jeong Ho Lee, Hyoung Jun Kim, Tae Sung Jung
Variable lymphocyte receptors B (VLRBs) are non-immunoglobulin components of the humoral immune system in jawless vertebrates including hagfish (Eptatretus burgeri) and lamprey (Petromyzon marinus). Hagfish VLRBs consist of leucine rich repeat (LRR) modules with a superhydrophobic C-terminal tail, the latter of which leads to extremely low expression levels in recombinant protein technology. Here, we present an artificially oligomerized VLRB (arVLRB) that conjugates via the C4bp oligomerization domain derived from human C4b-binding protein (hC4bp) rather than the superhydrophobic tail...
April 18, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29679762/scavenger-receptor-b-promotes-bacteria-clearance-by-enhancing-phagocytosis-and-attenuates-white-spot-syndrome-virus-proliferation-in-scylla-paramamosian
#2
Tongtong Kong, Yi Gong, Yan Liu, Xiaobo Wen, Ngoc Tuan Tran, Jude Juventus Aweya, Yueling Zhang, Hongyu Ma, Huaiping Zheng, Shengkang Li
Phagocytosis and apoptosis are key cellular innate immune responses against bacteria and virus in invertebrates. Class B scavenger receptors (SRBs), which contain a CD36 domain, are critical pattern recognition receptors (PRRs) of phagocytosis for bacteria and apoptotic cells. In the present study, we identified a member of SRB subfamily in mud crab Scylla paramamosain, named Sp-SRB. The full-length cDNA of Sp-SRB is 2593 bp with a 1629 bp open reading frame (ORF) encoding a putative protein of 542 amino acids, and predicted to contain a CD36 domain with two transmembrane regions at the C- and N-terminals...
April 18, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29679760/two-macrophage-migration-inhibitory-factors-mifs-from-the-clam-ruditapes-philippinarum-molecular-characterization-localization-and-enzymatic-activities
#3
Dan Wang, Dinglong Yang, Qing Wang, Ye Zhao, Chenghua Li, Qianyu Wei, Yijing Han, Jianmin Zhao
Macrophage migration inhibitory factor (MIF) is an evolutionarily ancient cytokine-like factor and plays a critical role in both innate and adaptive immunity. In the present study, two MIFs (designed as RpMIF-1 and RpMIF-2, respectively) were identified and characterized from the clam Ruditapes philippinarum by rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of RpMIF-1 and RpMFI-2 consisted of 531 and 722 nucleotides, encoding a polypeptide of 113 and 114 amino acid residues, respectively...
April 18, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29679649/protein-solvent-interfaces-in-human-y145stop-prion-protein-amyloid-fibrils-probed-by-paramagnetic-solid-state-nmr-spectroscopy
#4
Darryl Aucoin, Yongjie Xia, Theint Theint, Philippe S Nadaud, Krystyna Surewicz, Witold K Surewicz, Christopher P Jaroniec
The C-terminally truncated Y145Stop variant of prion protein (PrP23-144), which is associated with heritable PrP cerebral amyloid angiopathy in humans and also capable of triggering a transmissible prion disease in mice, serves as a useful in vitro model for investigating the molecular and structural basis of amyloid strains and cross-seeding specificities. Here, we determine the protein-solvent interfaces in human PrP23-144 amyloid fibrils generated from recombinant 13 C,15 N-enriched protein and incubated in aqueous solution containing paramagnetic Cu(II)-EDTA, by measuring residue-specific 15 N longitudinal paramagnetic relaxation enhancements using two-dimensional magic-angle spinning solid-state NMR spectroscopy...
April 18, 2018: Journal of Structural Biology
https://www.readbyqxmd.com/read/29679607/process-intensification-of-core-streptavidin-production-through-high-cell-density-cultivation-of-recombinant-e-coli-and-a-temperature-based-refolding-method
#5
Leong Huat Chua, Soo Choon Tan, Mervyn W O Liew
An intensified process was developed that enables high level production of recombinant core streptavidin (cSAV), a non-glycosylated tetrameric protein utilised in a wide range of applications. A pH-stat fed-batch feeding strategy was employed to achieve high-cell-density and improve volumetric yield of cSAV which was expressed as inclusion bodies (IBs). The effect of induction at different cell densities (OD 20, 60 and 100) on volumetric and specific yield were then studied. Highest volumetric yield of cSAV (1550 mg L-1 ) was obtained from induction at OD 100 without significant reductions in specific yield...
April 18, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29679478/randomized-controlled-study-of-bleselumab-askp1240-pharmacokinetics-and-safety-in-patients-with-moderate-to-severe-plaque-psoriasis
#6
Mysore S Anil Kumar, Kim Papp, Ryo Tainaka, Udaya Valluri, Xuegong Wang, Tong Zhu, Christian Schwabe
This study evaluated pharmacokinetics (PK), efficacy, safety, and tolerability of bleselumab - a fully-human anti-CD40 monoclonal recombinant IgG4. Patients with moderate-to-severe psoriasis were randomized on Day 1 to receive bleselumab or placebo on Days 1, 15, and 29 in a dose-escalation of bleselumab 0.1, 0.3, 1.0, or 3.0mg/kg. The safety-analysis set (SAF) and full-analysis set (FAS) included all patients who received bleselumab or placebo, and the PK-analysis set (PKAS) included patients in the SAF with ≥1 quantifiable serum bleselumab concentration...
April 21, 2018: Biopharmaceutics & Drug Disposition
https://www.readbyqxmd.com/read/29679312/efficient-production-of-secretory-streptomyces-clavuligerus-%C3%AE-lactamase-inhibitory-protein-blip-in-pichia-pastoris
#7
Kin-Ho Law, Man-Wah Tsang, Yuk-Ki Wong, Ming-San Tsang, Pui-Yee Lau, Kwok-Yin Wong, Kwok-Ping Ho, Yun-Chung Leung
β-Lactamase inhibitory protein (BLIP), a low molecular weight protein from Streptomyces clavuligerus, has a wide range of potential applications in the fields of biotechnology and pharmaceutical industry because of its tight interaction with and potent inhibition on clinically important class A β-lactamases. To meet the demands for considerable amount of highly pure BLIP, this study aimed at developing an efficient expression system in eukaryotic Pichia pastoris (a methylotrophic yeast) for production of BLIP...
April 20, 2018: AMB Express
https://www.readbyqxmd.com/read/29678922/enzymatic-synthesis-of-6-sialyllactose-a-dominant-sialylated-human-milk-oligosaccharide-by-a-novel-exo-%C3%AE-sialidase-from-bacteroides-fragilis-nctc9343
#8
Longcheng Guo, Xiaodi Chen, Li Xu, Min Xiao, Lili Lu
Gut bacteria provide a rich source of glycosidases that can recognize and/or hydrolyze glycans for nutritions. Interestingly, some glycosidases are also found to catalyze transglycosylation reactions in vitro and thus can be used for oligosaccharide synthesis. In this work, six putative and one known exo -α-sialidase genes, including three from Bacteroides fragilis NCTC9343, three from Clostridium perfringens ATCC13124, and one known from Bifidobacterium bifidum JCM1254, were subjected to gene cloning and heterogeneous expression in Escherichia coli The recombinant enzymes were purified, characterized for substrate specificity, and screened for transglycosylation activity...
April 20, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29678917/genomic-inference-of-recombination-mediated-evolution-in-xanthomonas-euvesicatoria-and-x-perforans
#9
Mustafa O Jibrin, Neha Potnis, Sujan Timilsina, Gerald V Minsavage, Gary E Vallad, Pamela D Roberts, Jeffrey B Jones, Erica M Goss
Recombination is a major driver of evolution in bacterial populations because it can spread and combine independently evolved beneficial mutations. Recombinant lineages of plant bacterial pathogens are typically associated with colonization of novel hosts and emergence of new diseases. Here, we show that recombination between evolutionarily and phenotypically distinct plant pathogenic lineages generated recombinant lineages with unique combinations of pathogenicity and virulence factors. X. euvesicatoria ( Xe ) and X...
April 20, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29678884/physiological-temperatures-reduce-dimerization-of-dengue-and-zika-virus-recombinant-envelope-proteins
#10
Stephan T Kudlacek, Lakshmanane Premkumar, Stefan W Metz, Ashutosh Tripathy, Andrey A Bobkov, Alexander Matthew Payne, Stephen Graham, James A Brackbill, Michael J Miley, Aravinda M de Silva, Brian Kuhlman
The spread of dengue (DENV) and Zika virus (ZIKV) is a major public health concern. The primary target of antibodies (Abs) that neutralize DENV and ZIKV is the envelope (E) glycoprotein, and there is interest in using soluble recombinant E (sRecE) proteins as subunit vaccines. However, the most potent neutralizing antibodies against DENV and ZIKV recognize epitopes on the virion surface that span two or more E proteins. Therefore, to create effective DENV and ZIKV vaccines, presentation of these quaternary epitopes may be necessary...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678793/characterisation-and-functional-comparison-of-single-crd-and-multidomain-containing-galectins-cggal-2-and-cggal-3-from-oyster-crassostrea-gigas
#11
Mengmeng Huang, Tao Zhou, Yuehong Wu, Hui Fei, Gaoyang Wang, Zhi Li, Yutong Lei, Qian Liu, Cong Sun, Zhengbing Lv, Xue-Wei Xu
Galectins are β-galactoside binding lectins that play crucial roles in innate immunity in vertebrates and invertebrates through their conserved carbohydrate-recognition domains (CRDs). In the present study, single- and four-CRD-containing galectins were identified in oyster Crassostrea gigas (designated CgGal-2 and CgGal-3). The open reading frames (ORFs) of CgGal-2 and CgGal-3 encode polypeptides of 200 and 555 amino acids, respectively. All CRDs of CgGal-3 include two consensus motifs essential for ligand-binding, and a novel motif is present in CgGal-2...
April 17, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29678789/molecular-modeling-and-docking-of-recombinant-hap-phytase-of-a-thermophilic-mould-sporotrichum-thermophile-reveals-insights-into-molecular-catalysis-and-biochemical-properties
#12
Bijender Singh, K K Sharma, Amit Kumari, Anil Kumar, S K Gakhar
A thermostable and protease-resistant HAP-phytase of Sporotrichum thermophile was over-expressed in Pichia pastoris ×33. Purified recombinant phytase displayed all its biochemical properties similar to wild type. Molecular modeling and docking of phytase with various substrates showed differential binding patterns with GoldScore values ranging from 40.61 to 79.78. Docking with different substrates revealed strong binding affinity with ATP and phytic acid, while the lowest with AMP and phosphoenol pyruvate...
April 17, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29678741/identification-and-characterization-of-an-endolysin-like-from-bacillus-subtilis
#13
Hossein Noormohammdi, Shamsozoha Abolmaali, Shakiba Darvish Alipour Astaneh
Drug-resistant Gram-positive pathogens have been a rising risk in hospitals and food industries from the last decades. Here in, the potential of endolysin production in Dasht Desert Bacterial Culture Collection (DDBCC), against indicator bacteria, was investigated. DDBCC was screened against autoclaved-indicator bacteria; Streptococcus faecalis, Streptococcus pyogenes, Bacillus sp, Bacillus subtilis and Staphylococcus aureus as the substrates for the endolysin enzymes. The endolysins were produced in BHI medium followed by ammonium sulfate purification...
April 17, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29678737/a-novel-purification-procedure-for-recombinant-human-serum-albumin-expressed-in-pichia-pastoris
#14
Shijie Li, Linlin Li, Zhuo Chen, Guangpu Xue, Longguang Jiang, Ke Zheng, Jincan Chen, Rui Li, Cai Yuan, Mingdong Huang
Plasma-derived human serum albumin (pHSA) has important applications in many clinical indications, including blood loss, serious burn, or hemorrhagic shock. The limited supply and potential infectious pathogen contamination of pHSA have stimulated the development of recombinant human serum albumin (rHSA). However, rHSA often entraps endogenous or exogenous impurities, including color pigments and polysaccharides. Therefore, the purification of rHSA to high purity remains the bottleneck in large-scale production of rHSA...
April 17, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29678736/effect-of-rare-codons-in-c-terminal-of-green-fluorescent-protein-on-protein-production-in-escherichia-coli
#15
Yaru Yan, Xiaoqing Liu, Qingbin Li, Xiaoyu Chu, Jian Tian, Ningfeng Wu
In the previous study, the results on two interesting egfp genes indicated that the expressed eGFP production of egfp-codon containing multiple rare codons was 2.3-fold than that of egfp-genscript with mainly high-frequency-usage codons. Therefore, the rare codons also play important roles for the functional expression of genes and it is interesting to know which rare codons in the egfp affect the functional expression of eGFP. In this study, the structure-guided SCHEMA recombination method and site-specific mutagenesis were proposed to detect the contribution of the rare codons on the functional expression of eGFP...
April 17, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29678725/cloning-and-characterization-of-a-second-lamprey-pituitary-glycoprotein-hormone-thyrostimulin-gpa2-gpb5
#16
Krist N Hausken, Belen Tizo, Michal Shpilman, Shannon Barton, Wayne Decatur, David Plachetzki, Scott Kavanaugh, Sabah Ul-Hasan, Berta Levavi-Sivan, Stacia A Sower
A novel heterodimeric glycoprotein hormone (GpH) comprised of alpha (GpA2) and beta (GpB5) subunits was discovered in 2002 and called thyrostimulin for its ability to activate the TSH receptor in mammals, but its central function in vertebrates has not been firmly established. We report here the cloning and expression of lamprey (l)GpB5, and its ability to heterodimerize with lGpA2 to form a functional l-thyrostimulin. The full-length cDNA of lGpB5 encodes 174 amino acids with ten conserved cysteine residues and one glycosylation site that is conserved with other vertebrate GpB5 sequences...
April 17, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29678695/the-imd-pathway-regulates-lysozyme-like-proteins-llps-in-the-silkmoth-antheraea-mylitta
#17
Valluri V Satyavathi, Amr A Mohamed, Swetha Kumari, Dadala M Mamatha, Bernard Duvic
Lysozyme-like proteins (LLPs) are members of the glycoside hydrolase family 22 (CAZY GH22). Unlike conventional c-type lysozymes (EC 3.2.1.17), LLPs lack specific catalytic amino acid residues essential for muramidase activity. Previous reports indicated upregulation of LLPs upon bacterial infection in the wild silkworm, Antheraea mylitta as well as in the domesticated silkworm, Bombyx mori. In the present work, we studied the signaling pathways mediating the production of LLPs using RNA interference-mediated knockdown of Spätzle, Relish and STAT, the key regulators of Toll, IMD (Immune deficiency) and JAK/STAT pathways, respectively...
April 17, 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29678627/structural-and-functional-insights-into-rha-p-a-bacterial-gh106-%C3%AE-l-rhamnosidase-from-novosphingobium-sp-pp1y
#18
Francesca Mensitieri, Federica De Lise, Andrea Strazzulli, Marco Moracci, Eugenio Notomista, Valeria Cafaro, Emiliano Bedini, Matthew Howard Sazinsky, Marco Trifuoggi, Alberto Di Donato, Viviana Izzo
α-L-Rhamnosidases (α-RHAs, EC 3.2.1.40) are glycosyl hydrolases (GHs) hydrolyzing terminal α-L-rhamnose residues from different substrates such as heteropolysaccharides, glycosylated proteins and natural flavonoids. Although the possibility to hydrolyze rhamnose from natural flavonoids has boosted the use of these enzymes in several biotechnological applications over the past decades, to date only few bacterial rhamnosidases have been fully characterized and only one crystal structure of a rhamnosidase of the GH106 family has been described...
April 17, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29678621/activation-of-the-non-canonical-nf-%C3%AE%C2%BAb-p52-pathway-in-vascular-endothelial-cells-by-rankl-elicits-pro-calcific-signalling-in-co-cultured-smooth-muscle-cells
#19
Emma Harper, Keith D Rochfort, Hannah Forde, Colin Davenport, Diarmuid Smith, Philip M Cummins
BACKGROUND: The intimal endothelium is known to condition the underlying medial smooth muscle cell (SMC) layer of the vessel wall, and is highly responsive to receptor-activator of nuclear factor-κB ligand (RANKL) and tumour necrosis factor-related apoptosis-inducing ligand (TRAIL), pro-calcific and anti-calcific agents, respectively. In this paper, we tested the hypothesis that RANKL-induced activation of endothelial NF-κB signalling is essential for pro-calcific activation of the underlying SMCs...
April 17, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29678586/ms-based-conformation-analysis-of-recombinant-proteins-in-design-optimization-and-development-of-biopharmaceuticals
#20
REVIEW
Devrishi Goswami, Jun Zhang, Pavel V Bondarenko, Zhongqi Zhang
Mass spectrometry (MS)-based methods for analyzing protein higher order structures have gained increasing application in the field of biopharmaceutical development. The predominant methods used in this area include native MS, hydrogen deuterium exchange-MS, covalent labeling, cross-linking and limited proteolysis. These MS-based methods will be briefly described in this article, followed by a discussion on how these methods contribute at different stages of discovery and development of protein therapeutics.
April 17, 2018: Methods: a Companion to Methods in Enzymology
keyword
keyword
41564
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"