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Caroline Maria Marcos, Haroldo Cesar de Oliveira, Julhiany de Fátima da Silva, Patricia Akemi Assato, Daniella Sayuri Yamazaki, Rosângela Aparecida Moraes da Silva, Cláudia Tavares Santos, Norival Alves Santos-Filho, Deivys Leandro Portuondo, Maria José Soares Mendes-Giannini, Ana Marisa Fusco-Almeida
Paracoccidioides spp., which are temperature-dependent dimorphic fungi, are responsible for the most prevalent human systemic mycosis in Latin America, the paracoccidioidomycosis. The aim of this study was to characterise the involvement of elongation factor Tu (EF-Tu) in Paracoccidioides brasiliensis-host interaction. Adhesive properties were examined using recombinant PbEF-Tu proteins and the respective polyclonal anti-rPbEF-Tu antibody. Immunogold analysis demonstrated the surface location of EF-Tu in P...
November 2016: FEMS Yeast Research
Xiaowan Wang, Hailong Li, Shinghua Ding
We previously demonstrated that Pre-B-cell colony-enhancing factor (PBEF), also known as nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme in mammalian NAD(+) biosynthesis pathway, plays a brain and neuronal protective role in ischemic stroke. In this study, we further investigated the mechanism of its neuroprotective effect after ischemia in the primary cultured mouse cortical neurons. Using apoptotic cell death assay, fluorescent imaging, molecular biology, mitochondrial biogenesis measurements and Western blotting analysis, our results show that the overexpression of PBEF in neurons can significantly promote neuronal survival, reduce the translocation of apoptosis inducing factor (AIF) from mitochondria to nuclei and inhibit the activation of capase-3 after glutamate-induced excitotoxicity...
2016: Scientific Reports
Ambra A Grolla, Cristina Travelli, Armando A Genazzani, Jaswinder K Sethi
In this review, we focus on the secreted form of nicotinamide phosphoribosyltransferase (NAMPT); extracellular NAMPT (eNAMPT), also known as pre-B cell colony-enhancing factor or visfatin. Although intracellular NAMPT is a key enzyme in controlling NAD metabolism, eNAMPT has been reported to function as a cytokine, with many roles in physiology and pathology. Circulating eNAMPT has been associated with several metabolic and inflammatory disorders, including cancer. Because cytokines produced in the tumour micro-environment play an important role in cancer pathogenesis, in part by reprogramming cellular metabolism, future improvements in cancer immunotherapy will require a better understanding of the crosstalk between cytokine action and tumour biology...
July 2016: British Journal of Pharmacology
Judith Z Drexler, Charles N Alpers, Leonid A Neymark, James B Paces, Howard E Taylor, Christopher C Fuller
In this paper, we provide the first record of millennial patterns of Pb and Hg concentrations on the west coast of the United States. Peat cores were collected from two micro-tidal marshes in the Sacramento-San Joaquin Delta of California. Core samples were analyzed for Pb, Hg, and Ti concentrations and dated using radiocarbon and (210)Pb. Pre-anthropogenic concentrations of Pb and Hg in peat ranged from 0.60 to 13.0μgg(-1)and from 6.9 to 71ngg(-1), respectively. For much of the past 6000+ years, the Delta was free from anthropogenic pollution, however, beginning in ~1425CE, Hg and Pb concentrations, Pb/Ti ratios, Pb enrichment factors (EFs), and HgEFs all increased...
May 1, 2016: Science of the Total Environment
Tereza Pavlová, Jan Novák, Julie Bienertová-Vašků
Visfatin (PBEF/Nampt) is an adipocytokine that exerts pleiotropic effects within the human body, particularly affecting its metabolism and immunity. Visfatin was originally identified as being secreted by peripheral blood lymphocytes acting as a pre-B-cell colony-enhancing factor (PBEF). However, it was subsequently reported to be expressed in almost every tissue of the human body, with visceral fat deposits being the main source of visfatin. In addition to its secreted form, visfatin may also be found intracellularly where it functions as a nicotinamide phosphoribosyltransferase (Nampt)...
November 2015: Journal of Reproductive Immunology
Ambra A Grolla, Simone Torretta, Ilaria Gnemmi, Angela Amoruso, Giuseppe Orsomando, Marco Gatti, Antonio Caldarelli, Dmitry Lim, Lorenza Penengo, Sandra Brunelleschi, Armando A Genazzani, Cristina Travelli
High plasma levels of nicotinamide phosphoribosyltransferase (NAMPT), traditionally considered an intracellular enzyme with a key role in NAD synthesis, have been reported in several oncological, inflammatory and metabolic diseases. We now show that eNAMPT can be actively released by melanoma cells in vitro. We analysed the mechanisms of its release, and we found both classical and non-classical pathway involvement. eNAMPT released by melanoma cells, in our hands, has paracrine and autocrine effects: it activates MAPK, AKT and NF-κB pathways and increases colony formation in anchorage-independent conditions...
November 2015: Pigment Cell & Melanoma Research
Wei Yang, Yuan Zeng, Bin Li, Jianliang Zhou, Yi Gong, Jianjun Xu, Xiao Dong
OBJECTIVE: This study aims to explore the relationship between PBEF and VEGF and p-MLC and the mechanism of PBEF increasing permeability of endothelial cells in hypoxia/re-oxygenation. METHODS: Hypoxia/re-oxygenation model was established and PBEF siRNA was synthesized. According to the different HUVEC treatment, it can be divided into normal control group, PBEF siRNA group; hypoxia (20 hours) and re-oxygenation (3 h) group, hypoxia (20 h) and re-oxygenation (6 h) group, hypoxia (20 h) and re-oxygenation (9 hours) group, hypoxia (20 h)/re-oxygenation (12 h)...
2015: International Journal of Clinical and Experimental Medicine
Sara M Camp, Ermelinda Ceco, Carrie L Evenoski, Sergei M Danilov, Tong Zhou, Eddie T Chiang, Liliana Moreno-Vinasco, Brandon Mapes, Jieling Zhao, Gamze Gursoy, Mary E Brown, Djanybek M Adyshev, Shahid S Siddiqui, Hector Quijada, Saad Sammani, Eleftheria Letsiou, Laleh Saadat, Mohammed Yousef, Ting Wang, Jie Liang, Joe G N Garcia
Ventilator-induced inflammatory lung injury (VILI) is mechanistically linked to increased NAMPT transcription and circulating levels of nicotinamide phosphoribosyl-transferase (NAMPT/PBEF). Although VILI severity is attenuated by reduced NAMPT/PBEF bioavailability, the precise contribution of NAMPT/PBEF and excessive mechanical stress to VILI pathobiology is unknown. We now report that NAMPT/PBEF induces lung NFκB transcriptional activities and inflammatory injury via direct ligation of Toll-like receptor 4 (TLR4)...
2015: Scientific Reports
Guang-Feng Ming, Xin-Hua Ma, Dao-Miao Xu, Zhi-Yong Liu, Yu-Hang Ai, Hui-Xia Liu, Zan-Hua Shi
Pre-B cell colony-enhancing factor (PBEF) has been shown to have a variety of biological functions. Studies have proven that PBEF plays a functional role in acute lung injury (ALI). Therefore, in this study, we aimed to confirm the importance of PBEF in ALI. The effects of PBEF overexpression on the apoptosis of human pulmonary microvascular endothelial cells (HPMECs) were analyzed by flow cytometry, and the results indicated that PBEF promoted the apoptosis of HPMECs, which aggravated the development of ALI...
September 2015: International Journal of Molecular Medicine
Z Y Gao, X Y Li, V Bhandari, L D Li, D Lan
Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening syndrome involving a final common pathway of hypercytokinemia, in which tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and soluble interleukin 2-receptor-alpha (sIL-2Rα) are the key cytokines. Pre-B-cell colony-enhancing factor (PBEF) is an inflammatory cytokine involved in several inflammatory diseases. However, its role in HLH is unknown. In this study, we examined the role of PBEF in HLH. Plasma was collected from 22 children with HLH and 14 healthy children...
2015: Genetics and Molecular Research: GMR
Katarzyna Neubauer, Iwona Bednarz Misa, Dorota Diakowska, Bartosz Kapturkiewicz, Andrzej Gamian, Malgorzata Krzystek-Korpacka
Targeting Nampt/PBEF/visfatin is considered a promising anticancer strategy, yet little is known about its association with colorectal cancer (CRC). We quantified Nampt/PBEF/visfatin expression in bowel and blood (mRNA and protein), referring it to CRC advancement and inflammatory, angiogenic, hypoxia, and proliferation indices. Tumor Nampt/PBEF/visfatin upregulation was associated with metastasis, anemia, tumor location, HIF1α, and inflammatory and angiogenic indices, of which HIF1α, IL1β, and anemia explained 70% in Nampt/PBEF/visfatin variability...
2015: BioMed Research International
Simon Kaja, Anna A Shah, Shamim A Haji, Krishna B Patel, Yuliya Naumchuk, Alexander Zabaneh, Bryan C Gerdes, Nancy Kunjukunju, Nelson R Sabates, Michael A Cassell, Ron K Lord, Kevin P Pikey, Abraham Poulose, Peter Koulen
The main objective of the study was to quantify serum levels of nicotinamide phosphoribosyltransferase (Nampt/pre-B-Cell colony-enhancing factor 1/visfatin) in subjects with a history of retinal vascular occlusions (RVOs), disease conditions characterized by pronounced ischemia, and metabolic energy deficits. A case-control study of 18 subjects with a history of RVO as well as six healthy volunteers is presented. Serum Nampt levels were quantified using a commercially available enzyme-linked immunosorbent assay kit...
2015: Clinical Ophthalmology
Yong-Hui Ding, Zhi-Wei Zhou, Chun-Fang Ha, Xue-Yu Zhang, Shu-Ting Pan, Zhi-Xu He, Jeffrey L Edelman, Dong Wang, Yin-Xue Yang, Xueji Zhang, Wei Duan, Tianxin Yang, Jia-Xuan Qiu, Shu-Feng Zhou
Ovarian cancer is a leading killer of women, and no cure for advanced ovarian cancer is available. Alisertib (ALS), a selective Aurora kinase A (AURKA) inhibitor, has shown potent anticancer effects, and is under clinical investigation for the treatment of advanced solid tumor and hematologic malignancies. However, the role of ALS in the treatment of ovarian cancer remains unclear. This study investigated the effects of ALS on cell growth, apoptosis, autophagy, and epithelial to mesenchymal transition (EMT), and the underlying mechanisms in human epithelial ovarian cancer SKOV3 and OVCAR4 cells...
2015: Drug Design, Development and Therapy
Sohaila Erfani, Mehdi Khaksari, Shahrbanoo Oryan, Nabi Shamsaei, Nahid Aboutaleb, Farnaz Nikbakht
Nicotinamide phosphoribosyl transferase/pre-B cell colony-enhancing factor/visfatin (Nampt/PBEF/visfatin) is an adipocytokine. By synthesizing nicotinamide adenine dinucleotide (NAD(+)), Nampt/PBEF/visfatin functions to maintain an energy supply that has critical roles in cell survival. Cerebral ischemia leads to energy depletion and eventually neuronal death by apoptosis in specific brain regions specially the hippocampus. However, the role of Nampt/PBEF/visfatin in brain and cerebral ischemia remains to be investigated...
May 2015: Journal of Molecular Neuroscience: MN
Qianyi Peng, Song Hui Jia, Jean Parodo, Yuhang Ai, John C Marshall
Pre-B cell colony-enhancing factor (PBEF) is a highly conserved pleiotropic protein reported to be an alternate ligand for the insulin receptor (IR). We sought to clarify the relationship between PBEF and insulin signaling by evaluating the effects of PBEF on the localization of the IRβ chain to lipid rafts in A549 epithelial cells. We isolated lipid rafts from A549 cells and detected the IR by immunoprecipitation from raft fractions or whole cell lysates. Cells were treated with rPBEF, its enzymatic product nicotinamide adenine dinucleotide (NAD), or the Nampt inhibitor daporinad to study the effect of PBEF on IRβ movement...
February 15, 2015: American Journal of Physiology. Endocrinology and Metabolism
Peter Bobbert, Uwe Kühl, Wolfgang Poller, Ursula Rauch, Heinz-Peter Schultheiss, Carsten Skurk
BACKGROUND: Nicotinamide phosphoribosyltransferase (Nampt) is an enzyme involved in nicotinamide adenine dinucleotide biosynthesis. Nampt functions as gatekeeper of energy status and survival in cardiac myocytes in animal models of ischemia-reperfusion and might regulate inflammatory processes. Therefore, we performed for the 1st time a clinical study to determine the effects of Nampt on cardiac function in patients with nonischemic dilated (DCM) and inflammatory (DCMi) cardiomyopathy...
April 2015: Journal of Cardiac Failure
Djanybek M Adyshev, Venkateswaran Ramamoorthi Elangovan, Nurgul Moldobaeva, Brandon Mapes, Xiaoguang Sun, Joe G N Garcia
Increased lung vascular permeability and alveolar edema are cardinal features of inflammatory conditions such as acute respiratory distress syndrome (ARDS) and ventilator-induced lung injury (VILI). We previously demonstrated that pre-B-cell colony-enhancing factor (PBEF)/NAMPT, the proinflammatory cytokine encoded by NAMPT, participates in ARDS and VILI inflammatory syndromes. The present study evaluated posttranscriptional regulation of PBEF/NAMPT gene expression in human lung endothelium via 3'-untranslated region (UTR) microRNA (miRNA) binding...
February 2014: American Journal of Respiratory Cell and Molecular Biology
Jiri Stastny, Julie Bienertova-Vasku, Josef Tomandl, Marie Tomandlova, Filip Zlamal, Martin Forejt, Zbynek Splichal, Anna Vasku
AIMS: Visfatin (NAMPT/PBEF) is a recently identified adipocytokine which harbors strong insulin-mimetic activity and was reported to be associated with obesity. However, nothing is known about whether visfatin is related to specific nutritional behavior which may result in obesity development. This is the first study focusing on genetic variability of the visfatin gene and its association with circulating visfatin, anthropometric parameters and dietary composition. MATERIALS AND METHODS: We analyzed a total of 11 exons and adjacent non-coding regions of the NAMPT gene in 20 extremely obese Czech individuals (mean BMI 52...
July 2013: Diabetes & Metabolic Syndrome
Tania Romacho, Carlos F Sánchez-Ferrer, Concepción Peiró
Adipose tissue is acknowledged as an endocrine organ that releases bioactive factors termed adipokines. Visfatin was initially identified as a novel adipokine with insulin-mimetic properties in mice. This adipokine was identical to two previously described molecules, namely, pre-B cell colony-enhancing factor (PBEF) and the enzyme nicotinamide phosphoribosyltransferase (Nampt). Enhanced circulating visfatin/Nampt levels have been reported in metabolic diseases, such as obesity and type 2 diabetes. Moreover, visfatin/Nampt circulating levels correlate with markers of systemic inflammation...
2013: Mediators of Inflammation
Kate J Roberts, Andrew Cross, Olga Vasieva, Robert J Moots, Steven W Edwards
NAMPT, also known as PBEF and visfatin, can act extracellularly as a cytokine-like molecule or intracellularly as a NAMPT, regulating NAD biosynthesis in the NAD salvage pathway. Inhibitors of NAMPT have anti-inflammatory and anticancer activity and are finding use as therapeutic agents. In view of the importance of NAD metabolism in neutrophil function, we determined the effects of NAMPT inhibition on a variety of neutrophil functions associated with their role in host protection against infections. Incubation of human neutrophils with the NAMPT inhibitor APO866 decreased neutrophil NAD(P)/H levels in a dose- and time-dependent manner but without a concomitant change in cell viability...
September 2013: Journal of Leukocyte Biology
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