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https://www.readbyqxmd.com/read/28110221/simultaneous-determination-of-eight-flavonoids-in-plasma-using-lc-ms-ms-and-application-to-a-pharmacokinetic-study-after-oral-administration-of-pollen-typhae-extract-to-rats
#1
Xie-An Yu, John Teye Azietaku, Jin Li, Jun Cao, Mingrui An, Jun He, Xiu-Mei Gao, Yan-Xu Chang
A sensitive, reliable and validated LC-MS/MS method was developed to determine the presence of eight flavonoids (catechin, typhaneoside, isorhamnetin-3-O-neohesperidoside, astragalin, isorhamnetin-3-O-β-d-glucoside, naringenin, kaempferol and isorhamnetin) in rat plasma. Puerarin was selected as the internal standard. Precipitation of the protein method with acetonitrile was used to extract these flavonoids from the rat plasma samples. The analysis was carried out on an Eclipse plus C18 column (4.6 mm×100mm, 1...
January 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28110062/metal-free-hybrids-of-graphitic-carbon-nitride-and-nanodiamonds-for-photoelectrochemical-and-photocatalytic-applications
#2
Li Zhou, Huayang Zhang, Xiaochen Guo, Hongqi Sun, Shaomin Liu, Moses O Tade, Shaobin Wang
Graphitic carbon nitride (g-C3N4) has been considered as a metal-free, cost-effective, eco-friendly and efficient catalyst for various photoelectrochemical applications. However, compared to conventional metal-based photocatalysts, its photocatalytic activity is still low because of the low mobility of carriers restricted by the polymer nature. Herein, a series of hybrids of g-C3N4 (GCN) and nanodiamonds (NDs) were synthesized using a solvothermal method. The photoelectrochemical performance and photocatalytic efficiency of the GCN/NDs were investigated by means of the generation of photocurrent and photodegradation of methylene blue (MB) solutions under UV-visible light irradiations...
January 12, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28109883/probing-transport-of-charged-%C3%AE-lactamase-inhibitors-through-ompc-a-membrane-channel-from-e-coli
#3
Ishan Ghai, Mathias Winterhalter, Richard Wagner
One of the major causes of antibiotic resistance in the Gram-negative bacteria is the low permeability across the outer membrane. Currently a main bottleneck in the development of effective antibiotics is the lack of a general method to quantify permeation which would allow screening for optimal scaffolds. Here, we present a permeation assay based on conventional electrophysiology. The method mainly involves application of concentration gradients of charged molecules with different electrophoretic mobilities through a membrane channel...
January 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28107701/simultaneous-determination-of-amantadine-rimantadine-and-memantine-in-processed-products-chicken-tissues-and-eggs-by-liquid-chromatography-with-tandem-mass-spectrometry
#4
Yumi Tsuruoka, Takayuki Nakajima, Maki Kanda, Hiroshi Hayashi, Yoko Matsushima, Souichi Yoshikawa, Marie Nagata, Hiroshi Koike, Chieko Nagano, Kotaro Sekimura, Tsuneo Hashimoto, Ichiro Takano, Tetsuya Shindo
A simultaneous determination of amantadine, rimantadine, and memantine in processed products (deep-fried chicken, fried chicken, fried quail egg, and grilled chicken) with liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. This new method was also applicable for chicken tissue (muscle, liver, and gizzard) and eggs. The chromatographic separation was performed on a Kinetex(®) XB-C18 core-shell technology column using a mobile phase of acetonitrile and 0.1% formic acid in a 10mmol/L ammonium formate solution, resulting in the complete separation of isomers (rimantadine and memantine) and any other obstructive peaks from the sample matrices...
January 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28107689/development-and-validation-of-a-liquid-chromatography-tandem-mass-spectrometry-method-for-the-assay-of-tafamidis-in-rat-plasma-application-to-a-pharmacokinetic-study-in-rats
#5
Hun-Chan Hyun, Jong-Woo Jeong, Hye-Rim Kim, Ji-Hoon Oh, Jong-Hwa Lee, Sungwook Choi, Yeon-Soo Kim, Tae-Sung Koo
Tafamidis is a first-in-class inhibitor of transthyretin amyloid fibril formation. It has been available in Argentina, Japan, and Mexico for the treatment of transthyretin amyloidosis in adult patients with early-stage symptomatic polyneuropathy. In this study, a rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the assay of tafamidis in rat plasma. The method was also assessed for its applicability to pharmacokinetic studies in rats. Tafamidis was extracted from rat plasma by the liquid-liquid extraction method using hydrochloric acid and ethyl acetate...
January 10, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28107391/recreational-drug-use-among-chinese-msm-and-transgender-individuals-results-from-a-national-online-cross-sectional-study
#6
Peizhen Zhao, Songyuan Tang, Cheng Wang, Ye Zhang, John Best, Thitikarn May Tangthanasup, Shujie Huang, Bin Yang, Chongyi Wei, Joseph D Tucker, Weiming Tang
BACKGROUND: Recreational drug use has increased considerably among Chinese men who have sex with men (MSM). The phenomenon has the potentially to increase HIV transmission among Chinese MSM. The aims of this study were: 1) to investigate the prevalence of recreational drug use among Chinese MSM, and 2) to explore the correlation between gay smartphone based sex-seeking applications (gay apps), HIV/STIs testing, group sex, commercial sex, sexual roles and poppers use among Chinese MSM...
2017: PloS One
https://www.readbyqxmd.com/read/28107258/what-to-eat-and-drink-in-the-festive-season-a-pan-european-observational-cross-sectional-study
#7
Helen L Parker, Jelena Curcic, Henriette Heinrich, Matthias Sauter, Michael Hollenstein, Werner Schwizer, Edoardo Savarino, Mark Fox
BACKGROUND: Digestive discomfort after meals is common in the community, especially during the festive season. It is uncertain whether this is related to intake of either high-calorie or high-fat foods or, alternatively, intake of specific foods. This prospective, cross-sectional study tested the hypothesis that the risk of reflux or dyspepsia is associated with the fat content of the meal independent of caloric load in a 'real-life' setting. MATERIALS AND METHODS: Four festive meals were served to delegates attending a conference on four consecutive days...
January 19, 2017: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28104499/methylene-blue-increases-the-amount-of-hsf1-through-promotion-of-pka-mediated-increase-in-hsf1-p300-interaction
#8
Chao Huang, Wenfeng Hu, Jili Wang, Lijuan Tong, Xu Lu, Feng Wu, Yong Ling, Bo Jiang, Wei Zhang, Zhuo Chen, Yibin Qin, Rongrong Yang
Heat shock factor 1 (HSF1) critically contributes to the host defense, and its amount determines the mobilization efficiency of HSF1 under stress conditions. To date, how HSF1 amount is regulated remains largely unknown. We found that methylene blue (MB), an anti-oxidative and anti-inflammatory agent, increased the amount of HSF1 in BV-2 microglia, primary microglia, astrocytes, neurons and vital organs. The increased HSF1 contributed to a more increase in nuclear translocation of HSF1, association of HSF1 with heat shock protein 70 (Hsp70) promoters and Hsp70 expression levels, and also induced a synergistic protection against oxidative stress-induced injuries in MB and heat shock-treated cells...
January 16, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28103776/small-and-hungry-micrornas-in-micronutrient-homeostasis-of-plants
#9
Prashanti Patel, Karuna Yadav, Thumballi Ramabhatta Ganapathi
MicroRNAs are emerging players in plant development and response to stresses, both biotic and abiotic such as micronutrient deficiency. These small RNAs regulate cognate downstream targets either by transcript cleavage or translational inhibition. Micronutrient deficiencies lead to poor quality and yield of crops and impaired human health. Over the years several microRNAs have been identified which regulate expression of genes controlling uptake, mobilization and homeostasis of macronutrients such as nitrogen, phosphorus and sulfur to ensure sufficiency without toxicity...
January 17, 2017: MicroRNA
https://www.readbyqxmd.com/read/28103760/the-use-of-a-direct-manufacturing-prosthetic-socket-system-in-a-rural-community-in-south-africa-a-pilot-study-and-lessons-for-future-research
#10
Liezel Ennion, Anton Johannesson, Anthea Rhoda
BACKGROUND: Challenges exist with the provision of appropriate mobility assistive devices in rural areas. The use of the direct manufacturing prosthetic socket system is a possible solution to these challenges. OBJECTIVES: The objective of this study was to test and explore the clients' perspectives with the application of this device. STUDY DESIGN: Within a mixed-methods approach, a longitudinal sequential explanatory design was applied. METHODS: The Orthotic and Prosthetic User's Survey was administered to explore the use of the direct manufacturing prosthetic socket system in terms of function, health-related quality of life and client satisfaction...
January 1, 2017: Prosthetics and Orthotics International
https://www.readbyqxmd.com/read/28103196/sensor-based-gait-parameter-extraction-with-deep-convolutional-neural-networks
#11
Julius Hannink, Thomas Kautz, Cristian Pasluosta, Karl-Gunter Gassmann, Jochen Klucken, Bjoern Eskofier
Measurement of stride-related, biomechanical parameters is the common rationale for objective gait impairment scoring. State-of-the-art double integration approaches to extract these parameters from inertial sensor data are, however, limited in their clinical applicability due to the underlying assumptions. To overcome this, we present a method to translate the abstract information provided by wearable sensors to context-related expert features based on deep convolutional neural networks. Regarding mobile gait analysis, this enables integration-free and data-driven extraction of a set of eight spatio-temporal stride parameters...
December 8, 2016: IEEE Journal of Biomedical and Health Informatics
https://www.readbyqxmd.com/read/28101781/seeking-medical-information-using-mobile-apps-and-the-internet-are-family-caregivers-different-from-the-general-public
#12
Hyunmin Kim, M Paige Powell, Soumitra S Bhuyan, Soumitra Sudip Bhuyan
Family caregivers play an important role to care cancer patients since they exchange medical information with health care providers. However, relatively little is known about how family caregivers seek medical information using mobile apps and the Internet. We examined factors associated with medical information seeking by using mobile apps and the Internet among family caregivers and the general public using data from the 2014 Health Information National Trends Survey 4 Cycle 1. The study sample consisted of 2425 family caregivers and 1252 non-family caregivers (the general public)...
March 2017: Journal of Medical Systems
https://www.readbyqxmd.com/read/28100957/conditions-for-success-in-introducing-telemedicine-in-diabetes-foot-care-a-qualitative-inquiry
#13
Beate-Christin Hope Kolltveit, Eva Gjengedal, Marit Graue, Marjolein M Iversen, Sally Thorne, Marit Kirkevold
BACKGROUND: The uptake of various telehealth technologies to deliver health care services at a distance is expanding; however more knowledge is needed to help understand vital components for success in using telehealth in different work settings. This study was part of a larger trial designed to investigate the effect of an interactive telemedicine platform. The platform consisted of a web based ulcer record linked to a mobile phone to provide care for people with diabetic foot ulcers in outpatient clinics in specialist hospital care in collaboration with primary health care...
2017: BMC Nursing
https://www.readbyqxmd.com/read/28100441/assessing-the-efficacy-of-an-app-based-method-of-family-planning-the-dot-study-protocol
#14
Rebecca G Simmons, Dominick C Shattuck, Victoria H Jennings
BACKGROUND: Some 222 million women worldwide have unmet needs for contraception; they want to avoid pregnancy, but are not using a contraceptive method, primarily because of concerns about side effects associated with most available methods. Expanding contraceptive options-particularly fertility awareness options that provide women with information about which days during their menstrual cycles they are likely to become pregnant if they have unprotected intercourse-has the potential to reduce unmet need...
January 18, 2017: JMIR Research Protocols
https://www.readbyqxmd.com/read/28099711/introducing-the-world-health-organization-postpartum-family-planning-compendium
#15
Sarita Sonalkar, Mary E Gaffield
The postpartum period offers multiple opportunities for healthcare providers to assist with family planning decision making. However, there are also many changing factors during the first year after delivery that can affect family planning choices. Given that several different documents have addressed WHO guidance on postpartum family planning, the electronic WHO Postpartum Family Planning Compendium (http://srhr.org/postpartumfp) has been introduced. This resource integrates essential guidance on postpartum family planning for clinicians, program managers, and policy makers...
January 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28099398/utilizing-a-novel-mobile-health-selfie-application-to-improve-compliance-to-iron-chelation-in-pediatric-patients-receiving-chronic-transfusions
#16
Sarah Leonard, Lindsay M Anderson, Jude Jonassaint, Charles Jonassaint, Nirmish Shah
Iron chelation therapy can prevent iron overload for pediatric patients with sickle cell disease and β-thalassemia major; however, adherence is suboptimal. Therefore, we developed an intensive training program (ITP), to improve medication management and disease knowledge. The objectives were to determine feasibility of the ITP and its preliminary impact on adherence, disease knowledge, and health outcomes. Pediatric patients were recruited to participate in the ITP over a 90-day period and were followed for 6 months...
January 17, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28099030/ultrafast-broadband-photodetectors-based-on-three-dimensional-dirac-semimetal-cd3as2
#17
Qinsheng Wang, Cai-Zhen Li, Shaofeng Ge, Jin-Guang Li, Wei Lu, Jiawei Lai, Xuefeng Liu, Junchao Ma, Dapeng Yu, Zhi-Min Liao, Dong Sun
Photodetection with extreme performances in terms of ultrafast response time, broad detection wavelength range, and high sensitivity has wide range of optoelectronic and photonic applications, such as optical communications, interconnects, imaging and remote sensing. Graphene, a typical two-dimensional Dirac semimetal, has shown excellent potential toward high performance photodetector with high operation speed, broadband response and efficient carrier multiplications benefiting from its linear dispersion band structure with high carrier mobility and zero bandgap...
January 18, 2017: Nano Letters
https://www.readbyqxmd.com/read/28098975/development-of-spiro-cyclopenta-1-2-b-5-4-b-dithiophene-4-9-fluorene-based-a-%C3%AF-d-%C3%AF-a-small-molecules-with-different-acceptor-units-for-efficient-organic-solar-cells
#18
Wengong Wang, Ping Shen, Xinning Dong, Chao Weng, Guo Wang, Haijun Bin, Jing Zhang, Zhi-Guo Zhang, Yongfang Li
Three acceptor-π-donor-π-acceptor (A-π-D-π-A) small molecules (STFYT, STFRDN, and STFRCN) with spiro[cyclopenta[1,2-b:5,4-b']dithiophene-4,9'-fluorene] (STF) as the central donor unit, terthiophene as the π-conjugated bridge, indenedione, 3-ethylrhodanine, or 2-(1,1-dicyanomethylene)rhodanine as the acceptor unit are designed, synthesized and characterized as electron donor materials in solution-processing organic solar cells (OSCs). The effects of the spiro STF-based central core and different acceptors on the molecular configuration, absorption properties, electronic energy levels, carrier transport properties, the morphology of active layers, and photovoltaic properties are investigated in detail...
January 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28098772/a-non-intrusive-cyber-physical-social-sensing-solution-to-people-behavior-tracking-mechanism-prototype-and-field-experiments
#19
Yunjian Jia, Zhenyu Zhou, Fei Chen, Peng Duan, Zhen Guo, Shahid Mumtaz
Tracking people's behaviors is a main category of cyber physical social sensing (CPSS)-related people-centric applications. Most tracking methods utilize camera networks or sensors built into mobile devices such as global positioning system (GPS) and Bluetooth. In this article, we propose a non-intrusive wireless fidelity (Wi-Fi)-based tracking method. To show the feasibility, we target tracking people's access behaviors in Wi-Fi networks, which has drawn a lot of interest from the academy and industry recently...
January 13, 2017: Sensors
https://www.readbyqxmd.com/read/28098770/can-malaysian-young-adults-report-dietary-intake-using-a-food-diary-mobile-application-a-pilot-study-on-acceptability-and-compliance
#20
Yoke San Chen, Jyh Eiin Wong, Ainaa Fatehah Ayob, Nor Effendy Othman, Bee Koon Poh
Mobile applications may improve dietary reporting among young adults due to their high accessibility and embedded camera function. This pilot study aimed to (i) evaluate users' acceptability and compliance in reporting dietary intake using a newly developed food diary mobile application (food app); and (ii) identify issues and recommendations for improving dietary assessment using this food app via quantitative and qualitative protocols. Twenty-eight university students each used a food app for seven consecutive days and attended one of five focus group interviews...
January 13, 2017: Nutrients
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