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Shrimp anti-viral protein

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https://www.readbyqxmd.com/read/27497874/shrimp-with-knockdown-of-lvsocs2-a-negative-feedback-loop-regulator-of-jak-stat-pathway-in-litopenaeus-vannamei-exhibit-enhanced-resistance-against-wssv
#1
Sheng Wang, Xuan Song, Zijian Zhang, Haoyang Li, Kai L, Bin Yin, Jianguo He, Chaozheng Li
JAK/STAT pathway is one of cytokine signaling pathways and mediates diversity immune responses to protect host from viral infection. In this study, LvSOCS2, a member of suppressor of cytokine signaling (SOCS) families, has been cloned and identified from Litopenaeus vannamei. The full length of LvSOCS2 is 1601 bp, including an 1194 bp open reading frame (ORF) coding for a putative protein of 397 amino acids with a calculated molecular weight of ∼42.3 kDa. LvSOCS2 expression was most abundant in gills and could respond to the challenge of LPS, Vibrio parahaemolyticus, Staphhylococcus aureus, Poly (I: C) and white spot syndrome virus (WSSV)...
December 2016: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/27349204/three-members-of-ras-gtpase-superfamily-are-response-to-white-spot-syndrome-virus-challenge-in-marsupenaeus-japonicus
#2
Xin Huang, Ting Ye, Min Jin, Wen Wang, Kaimin Hui, Qian Ren
Members of the Ras-like GTPase superfamily are key regulators of diverse cellular and developmental events, including differentiation, cell division, vesicle transport, nuclear assembly, and cytoskeleton control. In this study, three Ras family members (MjRap, MjRas, and MjRal) were cloned from Marsupenaeus japonicus. The full lengths of MjRap, MjRas, and MjRal are 788, 1330, and 2074 bp, which encode the proteins of 186, 202, and 198 amino acids respectively. Phylogenetic analysis showed that Rap, Ras, and Ral from different species gather together...
August 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27177431/dietary-supplementation-of-probiotic-bacillus-pc465-isolated-from-the-gut-of-fenneropenaeus-chinensis-improves-the-health-status-and-resistance-of-litopenaeus-vannamei-against-white-spot-syndrome-virus
#3
Peng-Cheng Chai, Xiao-Ling Song, Guo-Fu Chen, Hua Xu, Jie Huang
This study conducted a 30-day feeding trial and a subsequent 20-day anti-virus infection trial to determine the effects of probiotic Bacillus PC465 on the growth, health status, and disease resistance of Litopenaeus vannamei. Shrimp samples were fed with three practical diets prepared from shrimp feed containing varying probiotic doses [0 (control), 10(7), and 10(9) CFU g(-1)]. Probiotic supplementation significantly increased the weight gain and survival of L. vannamei (p < 0.05). The effect of 10(9) CFU g(-1) on the growth rate was higher than that of 10(7) CFU g(-1)...
July 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/24607895/two-new-anti-apoptotic-proteins-of-white-spot-syndrome-virus-that-bind-to-an-effector-caspase-pmcasp-of-the-giant-tiger-shrimp-penaeus-penaeus-monodon
#4
Tareerat Lertwimol, Pakkakul Sangsuriya, Kornsunee Phiwsaiya, Saengchan Senapin, Amornrat Phongdara, Chuenchit Boonchird, Timothy W Flegel
White spot syndrome virus proteins WSSV134 and WSSV322 have been shown to bind with the p20 domain (residues 55-214) of Penaeus monodon caspase (PmCasp) protein through yeast two-hybrid screening. Binding was confirmed for the p20 domain and the full-length caspase by co-immunoprecipitation. WSSV134 is also known as the WSSV structural protein VP36A, but no function or conserved domains have been ascribed to WSSV322. Discovery of the caspase binding activity of these two proteins led to an investigation of their possible anti-apoptotic roles...
May 2014: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/24509436/sulfated-galactans-isolated-from-the-red-seaweed-gracilaria-fisheri-target-the-envelope-proteins-of-white-spot-syndrome-virus-and-protect-against-viral-infection-in-shrimp-haemocytes
#5
Tawut Rudtanatip, Somluk Asuvapongpatana, Boonsirm Withyachumnarnkul, Kanokpan Wongprasert
The present study was aimed at evaluating an underlying mechanism of the antiviral activity of the sulfated galactans (SG) isolated from the red seaweed Gracilaria fisheri against white spot syndrome virus (WSSV) infection in haemocytes of the black tiger shrimp Penaeus monodon. Primary culture of haemocytes from Penaeus monodon was performed and inoculated with WSSV, after which the cytopathic effect (CPE), cell viability and viral load were determined. Haemocytes treated with WSSV-SG pre-mix showed decreased CPE, viral load and cell mortality from the viral infection...
May 2014: Journal of General Virology
https://www.readbyqxmd.com/read/24198431/viral-micrornas-targeting-virus-genes-promote-virus-infection-in-shrimp-in-vivo
#6
Yaodong He, Kai Yang, Xiaobo Zhang
Viral microRNAs (miRNAs), most of which are characterized in cell lines, have been found to play important roles in the virus life cycle to avoid attack by the host immune system or to keep virus in the latency state. Viral miRNAs targeting virus genes can inhibit virus infection. In this study, in vivo findings in Marsupenaeus japonicus shrimp revealed that the viral miRNAs could target virus genes and further promote the virus infection. The results showed that white spot syndrome virus (WSSV)-encoded miRNAs WSSV-miR-66 and WSSV-miR-68 were transcribed at the early stage of WSSV infection...
January 2014: Journal of Virology
https://www.readbyqxmd.com/read/24056278/characterization-of-a-2-cys-peroxiredoxin-iv-in-marsupenaeus-japonicus-kuruma-shrimp-and-its-role-in-the-anti-viral-immunity
#7
Xiao-Wei Chen, Li-Hua Kang, Ding Ding, Qian Liu, Jin-Xing Wang, Cui-Jie Kang
Accumulating evidence suggests that peroxiredoxins (Prx) are key molecules in the pathogenesis of various infectious diseases and are potential therapeutic targets for major diseases such as cancers. In this study, we report a peroxiredoxin IV (Prx IV) in Marsupenaeus japonicus, designated as MjPrx IV, which exhibited peroxidase activity and participated in the anti-white spot syndrome virus (WSSV) immune response. MjPrx IV is a 245-amino acid polypeptide with a predicted 19-amino acid signal peptide, an Ahpc-TSA domain, and a 1-Cys PrxC domain...
December 2013: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/24045127/molecular-immune-response-of-the-american-lobster-homarus-americanus-to-the-white-spot-syndrome-virus
#8
K Fraser Clark, Spencer J Greenwood, Adam R Acorn, Philip J Byrne
The adult American lobster (Homarus americanus) is susceptible to few naturally occurring pathogens, and no viral pathogen is known to exist. Despite this, relatively little is known about the H. americanus immune system and nothing is known about its potential viral immune response. Hundreds of rural communities in Atlantic Canada rely on the lobster fishery for their economic sustainability and could be devastated by large-scale pathogen-mediated mortality events. The White Spot Syndrome Virus is the most economically devastating viral pathogen to global shrimp aquaculture production and has been proposed to be capable of infecting all decapod crustaceans including the European Lobster...
November 2013: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/23997442/host-and-virus-protein-interaction-studies-in-understanding-shrimp-virus-gene-function
#9
Subhendu Kumar Otta
Protein-protein interaction studies have been widely used in several fields to characterize an unknown protein. This in turn helps to find out several pathways to understand a complex mechanism or discover a drug for treatment. Among the methods, yeast two-hybrid has widely been used in human, animal and plant research studies. This aspect of research has also been found useful in understanding the shrimp virus gene function. With respect to White spot syndrome virus, interaction studies have been applied to elucidate virus structure, understand the mode of entry of the virus, mechanism of virus replication and also to discover some of the host anti-viral proteins...
September 2012: Indian Journal of Virology: An Official Organ of Indian Virological Society
https://www.readbyqxmd.com/read/23108256/the-vp37-binding-protein-f1atp-synthase-%C3%AE-subunit-involved-in-wssv-infection-in-shrimp-litopenaeus-vannamei
#10
Wenbin Zhan, Xiaolu Wang, Yanyan Chi, Xiaoqian Tang
To investigate the interaction between white spot syndrome virus (WSSV)-VP37 and gill membrane proteins (GMPs) of Pacific white shrimp (Litopenaeus vannamei), the VP37 protein was expressed and purified, and a distinct 53 kDa VP37-binding protein band was identified in GMPs by virus overlay protein binding assay and GST pull-down assay. By electroelution, the VP37 binding protein was purified and identified as F(1)ATP synthase β (F(1)ATPase β) subunit by Mass Spectrometry. The purified F(1)ATPase β subunit was used to immunize BALB/C mice to produce monoclonal antibodies (Mabs)...
January 2013: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/23015693/functional-analysis-of-a-crustacean-microrna-in-host-virus-interactions
#11
Tianzhi Huang, Xiaobo Zhang
Growing evidence from mammals suggests that host microRNAs (miRNAs) play important roles in the antiviral immune response. However, the roles of invertebrate miRNAs in response to virus infection remain to be investigated. Based on our previous studies, the shrimp miR-7 was found to be upregulated in response to white spot syndrome virus (WSSV) infection. In this study, the results showed that shrimp miR-7 could target the 3'-untranslated region (3'UTR) of the WSSV early gene wsv477, implying that miR-7 was involved in viral DNA replication...
December 2012: Journal of Virology
https://www.readbyqxmd.com/read/22643833/anti-white-spot-syndrome-virus-activity-of-ceriops-tagal-aqueous-extract-in-giant-tiger-shrimp-penaeus-monodon
#12
N S Sudheer, Rosamma Philip, I S Bright Singh
White spot syndrome virus (WSSV), the most contagious pathogen of cultured shrimp, causes mass mortality, leading to huge economic loss to the shrimp industry. The lack of effective therapeutic or prophylactic measures has aggravated the situation, necessitating the development of antiviral agents. With this objective, the antiviral activity in the aqueous extract of a mangrove plant Ceriops tagal in Penaeus monodon was evaluated. The Ceriops tagal aqueous extract (CTAE) was non-toxic to shrimps at 50 mg/ml when injected intramuscularly at a dosage of 10 μL/animal (0...
September 2012: Archives of Virology
https://www.readbyqxmd.com/read/22554476/identification-and-characterization-of-inositol-requiring-enzyme-1-and-x-box-binding-protein-1-two-proteins-involved-in-the-unfolded-protein-response-of-litopenaeus-vannamei
#13
Yi-Hong Chen, Li Zhao, Li-Ran Pang, Xiao-Yun Li, Shao-Ping Weng, Jian-Guo He
The inositol-requiring enzyme-1 (IRE1)-X-box binding protein 1 (IRE1-XBP1) pathway is the key branch of the unfolded protein response (UPR). To investigate the role of the IRE1-XBP1 pathway in reducing environmental stress and increasing anti-viral immunity in Litopenaeus vannamei, homologues of IRE1 (designated as LvIRE1) and XBP1 (designated as LvXBP1) were identified and characterized. The full-length cDNA of LvIRE1 is 4908bp long, with an open reading frame (ORF) that encodies a putative 1174 amino acid protein...
September 2012: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/22406449/blocking-the-large-extracellular-loop-lel-domain-of-fctetraspanin-3-could-inhibit-the-infection-of-white-spot-syndrome-virus-wssv-in-chinese-shrimp-fenneropenaeus-chinensis
#14
Lang Gui, Bing Wang, Fu-Hua Li, Yu-Miao Sun, Zhan Luo, Jian-Hai Xiang
Tetraspanins belong to the transmembrane 4 superfamily (TM(4)SF), which span the cell membrane 4 times and act as bridges or connectors. Increasing evidences have shown that tetraspanins play important role in virus infection. The large extracellular loop (LEL) of a tetraspanin is considered as a possible target of some virus. Tetraspanins are widely found in invertebrates, but the functional roles of most invertebrate tetraspanins have remained unknown. Recently, a tetraspanin, called FcTetraspanin-3, was cloned from the cDNA library of Chinese shrimp, Fenneropenaeus chinensis...
June 2012: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/22030024/gene-silencing-reveals-a-crucial-role-for-anti-lipopolysaccharide-factors-from-penaeus-monodon-in-the-protection-against-microbial-infections
#15
Sirikwan Ponprateep, Sirinit Tharntada, Kunlaya Somboonwiwat, Anchalee Tassanakajon
Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides previously identified in various crustaceans. Out of five isoforms identified in Penaeus monodon, ALFPm3 is the best characterized, exhibits antibacterial and antifungal activities and can protect the shrimp from viral infections. Herein, the most recent identified ALFPm, called ALFPm6, is characterized for its potential role in the shrimp's immunity. RNA interference-mediated gene silencing was used to study the function of ALFPm6 in comparison to ALFPm3...
January 2012: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/21741409/purification-of-infectious-myonecrosis-virus-imnv-in-species-of-marine-shrimp-litopenaeus-vannamei-in-the-state-of-cear%C3%A3
#16
Maria Verônyca Coelho Mello, Maria Erivalda Farias de Aragão, Maria Lúcia Pereira Torres-Franklin, João Mafaldo de Oliviera Neto, Maria Izabel Florindo Guedes
In Brazil, shrimp farming has been developed most intensely in the Northeast Region. Recently, however, exporters have become concerned over the appearance of Infectious Myonecrosis (IMN), the etiological agent of which is a virus called Infectious Myonecrosis Virus (IMNV). Although IMNV has been characterized extensively, purification methods are complicated to reproduce and very expensive. The objective of this study was to purify the IMNV virus using an easy reproductive method and to produce anti-IMNV antibodies to be used in diagnostic methods...
October 2011: Journal of Virological Methods
https://www.readbyqxmd.com/read/21586306/clearance-of-penaeus-monodon-densovirus-in-naturally-pre-infected-shrimp-by-combined-ns1-and-vp-dsrnas
#17
Pongsopee Attasart, Rossukon Kaewkhaw, Chaweewan Chimwai, Ukrit Kongphom, Sakol Panyim
Penaeus monodon densovirus (PmDNV) is one of the major causes of stunted shrimp in Thailand and leads to considerable economic losses in overall shrimp production. Present study shows that the double-stranded RNA corresponding to the non-structural protein gene (ns1) and structural protein gene (vp) of PmDNV effectively inhibit viral propagation in naturally pre-infected shrimp. Multiple application of dsRNA was performed by injection into the haemolymph. The total amount of virus in the hepatopancreas of treated shrimp was measured by semi-quantitative PCR and histological methods...
July 2011: Virus Research
https://www.readbyqxmd.com/read/21256869/simultaneous-and-rapid-detection-of-white-spot-syndrome-virus-and-yellow-head-virus-infection-in-shrimp-with-a-dual-immunochromatographic-strip-test
#18
Paisarn Sithigorngul, Sombat Rukpratanporn, Parin Chaivisuthangkura, Pattarin Sridulyakul, Siwaporn Longyant
A strip test for the dual detection of white spot syndrome virus (WSSV) and yellow head virus (YHV) was developed using monoclonal antibodies (MAbs) specific to the WSSV major envelope protein VP28 (W1 and W30) and the YHV nucleocapsid protein p20 (Y19 and Y21). The MAbs W30 and Y19 were conjugated with colloidal gold and sprayed onto a glass fiber pad that was placed adjacent to a sample chamber. The MAbs W1 and Y21 and the goat anti-mouse immunoglobulin G (GAM) antibody were sprayed onto a nitrocellulose membrane in strips at positions designated W, Y and C, respectively...
April 2011: Journal of Virological Methods
https://www.readbyqxmd.com/read/21256150/identification-and-functional-characterization-of-dicer2-and-five-single-vwc-domain-proteins-of-litopenaeus-vannamei
#19
Yi-Hong Chen, Xiao-Ting Jia, Li Zhao, Chao-Zheng Li, Shuang Zhang, Yong-Gui Chen, Shao-Ping Weng, Jian-Guo He
Dicer (Dcr) is the key protein of the RNA interference (RNAi) pathway. To investigate the role of the RNAi pathway in shrimp anti-viral immunity, Litopenaeus vannamei Dcr2 (designated as LvDcr2) was identified and characterized. The full-length cDNA of LvDcr2 was 5513bp long, with an open reading frame encoding a putative protein of 1502 amino acids. In addition, five proteins homologous to the single von Willebrand factor type C (VWC) domain protein (SVC) were also identified in L. vannamei and named LvSVC1-5...
June 2011: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/21192980/multiplication-of-taura-syndrome-virus-in-primary-hemocyte-culture-of-shrimp-penaeus-vannamei
#20
Sunil K George, Krista N Kaizer, Yelena M Betz, Arun K Dhar
The propagation of Taura syndrome virus (TSV) in primary hemocyte culture of Pacific white shrimp (Penaeus vannamei) was investigated. Purified TSV was inoculated into a 24 h old primary hemocyte culture and the development of cytopathic effects was monitored. The cell morphology started changing within 6 h post-inoculation; TSV-infected hemocytes started shrinking and granular structures began to form on the cell surface. There was a gradual loss of cell viability and, by 48 h post-inoculation, most cells detached from the bottom of the 96 well microplate...
March 2011: Journal of Virological Methods
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