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Shrimp immune system

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https://www.readbyqxmd.com/read/29146444/snps-in-the-toll1-receptor-of-litopenaeus-vannamei-are-associated-with-immune-response
#1
Defu Yao, Huimin Su, Jinghua Zhu, Xianliang Zhao, Jude Juventus Aweya, Fan Wang, Mingqi Zhong, Yueling Zhang
Tolls and Toll-like receptors (TLRs) are important regulators in the innate immune system and their genetic variations usually affect the host's susceptibility/resistance to pathogen infections. In this study, we report on the single nucleotide polymorphisms (SNPs) of Toll1 in Litopenaeus vannamei (LvToll1) and how this is associated with immune response. PCR-DGGE analysis revealed genetic polymorphisms in LvToll1 at both the genomic DNA (gDNA) and cDNA levels. Using high-throughput sequencing, 223 SNPs were identified at the gDNA level, of which 145 were non-synonymous SNP (nsSNP), with 3 nsSNPs having frequency over 1%...
November 13, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29128494/the-shrimp-hormone-receptor-acts-as-an-anti-apoptosis-and-anti-inflammatory-factor-in-innate-immunity
#2
Zhi Wang, Baozhen Sun, Fei Zhu
Previously, we found that the expression of several genes, including HR, varied in Drosophila melanogaster after white spot syndrome virus (WSSV) infection. In this present study, we further investigated the role of HR in Kuruma shrimp, Marsupenaeus japonicus and determined its anti-apoptosis and anti-inflammation role in the innate immune system. We successfully identified a partial sequence (866 bp in length) of the M. japonicus hormone receptor ligand binding domain (mjHR_LBD/mjHR). The 5' end of mjHR was successfully obtained; the open reading frame (ORF) ran from 33 to 701 bp, and encoded a protein containing 222 amino acids...
November 8, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29056496/the-spread-of-vibrio-parahaemolyticus-in-tissues-of-the-pacific-white-shrimp-litopenaeus-vannamei-analyzed-by-pcr-and-histopathology
#3
Umaporn Khimmakthong, Pimwarang Sukkarun
V. parahaemolyticus are bacteria that cause the Acute Hepatopancreatic Necrosis Disease (AHPND), or Early Mortality Syndrome (EMS), in shrimp. To further understand the pathogenesis mechanisms of V. parahaemolyticus infection in shrimp, the spreading of this bacterium in various tissues was investigated. The spread of infection in shrimp that were exposed to seawater bacteria was studied by PCR and histopathology at 1 min, 1, 6, 12, 24, 48 and 72 h after exposure. The PCR results showed that V. parahaemolyticus was at its most widespread at 6 h after exposure, at which point V...
October 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29055189/a-mannose-specific-c-type-lectin-from-fenneropenaeus-merguiensis-exhibited-antimicrobial-activity-to-mediate-shrimp-innate-immunity
#4
Phanthipha Runsaeng, Peerapong Puengyam, Prapaporn Utarabhand
Being one type of pattern recognition receptors (PRRs), lectins exhibit a crucial role in the defense mechanism of invertebrates which are deficient in an adaptive immune system. A new C-type lectin called FmLC3 was isolated from hepatopancreas of Fenneropenaeus merguiensis by cloning approaches, RT-PCR and 5' and 3' RACE (rapid amplification of cDNA ends). A full-length cDNA of FmLC3 contains 607 bp with one open reading frame of 480bp, encoding a 159-amino acids peptide. The predicted primary structure of FmLC3 is composed of a signal peptide, a carbohydrate recognition domain with an EPN motif and one Ca(2+) binding site-2, including a double-loop region assisted by two conserved disulfide linkages...
October 18, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29042192/an-immune-related-gene-expression-atlas-of-the-shrimp-digestive-system-in-response-to-two-major-pathogens-brings-insights-into-the-involvement-of-hemocytes-in-gut-immunity
#5
Amanda S Silveira, Gabriel M Matos, Marcelo Falchetti, Fabio S Ribeiro, Albert Bressan, Evelyne Bachère, Luciane M Perazzolo, Rafael D Rosa
Much of our current knowledge on shrimp immune system is restricted to the defense reactions mediated by the hemocytes and little is known about gut immunity. Here, we have investigated the transcriptional profile of immune-related genes in different organs of the digestive system of the shrimp Litopenaeus vannamei. First, the tissue distribution of 52 well-known immune-related genes has been assessed by semiquantitative analysis in the gastrointestinal tract (foregut, midgut and hindgut) and in the hepatopancreas and circulating hemocytes of shrimp stimulated or not with heat-killed bacteria...
October 14, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29032039/the-efficacy-of-poly-%C3%AE-hydroxy-butyrate-phb-biosurfactant-derived-from-staphylococcus-hominis-against-white-spot-syndrome-virus-wssv-in-penaeus-monodon
#6
M Monica, T Priyanka, Murugesan Akshaya, V Rajeswari, Lingappa Sivakumar, S T Somasundaram, R Shenbhagarathai
White Spot Syndrome Virus (WSSV) is one of the most important causative agents of Penaeid shrimps diseases that incur heavy losses to the shrimp aquaculture. It has severe impact on the sustainability and the production of Penaeus monodon. Hence, the present study focussed on the investigation of Poly-β-hydroxybutyrate/biosurfactant as immunostimulants against WSSV infected shrimps. Infection of WSSV was periodically checked in all the experimental shrimps using PCR diagnostic kit. After ensuring all shrimps were free of viral infection, experiments were carried out to analyze the nonspecific immune responses (prophenol oxidase, nitro blue tetrazolium reduction assay and total haemocyte count) both in control and experimental group...
October 12, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28986214/different-roles-of-a-novel-shrimp-microrna-in-white-spot-syndrome-virus-wssv-and-vibrio-alginolyticus-infection
#7
Zhi Wang, Fei Zhu
In this study, Marsupeneaus japonicus microRNA-S5 (miR-S5) was found to be up-regulated 24 h post white spot syndrome virus (WSSV) or V. alginolyticus infection. The loss of function using an anti-microRNA oligonucleotide (AMO-miR-S5) showed that expression levels of multiple innate immune-related genes were affected. The expression of p53 and tumor necrosis factor-α (TNF-α) were significantly down-regulated, expression of myosin was significantly up-regulated. The miR-S5 knockdown delayed WSSV-induced death for 48 h, but the final mortality was not affected, while V...
October 4, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28982619/toll-receptor-9-gene-in-the-black-tiger-shrimp-penaeus-monodon-induced-the-activation-of-the-tlr-nf-%C3%AE%C2%BAb-signaling-pathway
#8
Qian Liu, Xu Dan, Shigui Jiang, Jianhua Huang, Falin Zhou, Yang Qibin, Jiang Song, Lishi Yang
Toll receptors are important pathogen recognition receptors (PRRs) in shrimps, which play a vital role in defending against virus and bacterial challenge. In this paper, the characterization and functional analysis of a Toll9 receptor gene from Penaeus monodon was performed in HEK293T cells. Data showed that PmToll9 can activate the NF-κB promoter activities of TLR pathway, while ISRE and IFN-β promoter cannot be activated obviously in HEK293T cells using dual-luciferase reporter system. The downstream immune factors of IL-8, IκB-α, and TRAF6 were activated by PmToll9 and IL-8 showed the most significant up-regulation in expression levels, indicating the activities of NF-κB can be mediated by PmToll9...
October 2, 2017: Gene
https://www.readbyqxmd.com/read/28970971/advances-in-the-study-of-nodavirus
#9
Chean Yeah Yong, Swee Keong Yeap, Abdul Rahman Omar, Wen Siang Tan
Nodaviruses are small bipartite RNA viruses which belong to the family of Nodaviridae. They are categorized into alpha-nodavirus, which infects insects, and beta-nodavirus, which infects fishes. Another distinct group of nodavirus infects shrimps and prawns, which has been proposed to be categorized as gamma-nodavirus. Our current review focuses mainly on recent studies performed on nodaviruses. Nodavirus can be transmitted vertically and horizontally. Recent outbreaks have been reported in China, Indonesia, Singapore and India, affecting the aquaculture industry...
2017: PeerJ
https://www.readbyqxmd.com/read/28958586/fmlc5-a-putative-galactose-binding-c-type-lectin-with-two-qpd-motifs-from-the-hemocytes-of-fenneropenaeus-merguiensis-participates-in-shrimp-immune-defense
#10
Wilaiwan Senghoi, Phanthipha Runsaeng, Prapaporn Utarabhand
Crustaceans are deficient in adaptive immune system. They depend completely on an innate immunity to protect themselves from invading microorganisms. One kind of pattern recognition receptors that contribute roles in the innate immunity is lectin. A new C-type lectin gene designated as FmLC5 was isolated from Fenneropenaeus merguiensis. Its full-length cDNA is composed of 1526bp and one open reading frame of 852bp encoding a peptide of 284 amino acids. The deduced amino acid sequence of FmLC5 comprises a signal peptide of 20 contiguous amino acids with a molecular mass of 31...
September 25, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28939212/fortilin-a-potential-target-for-the-prevention-and-treatment-of-human-diseases
#11
Decha Pinkaew, Ken Fujise
Fortilin is a highly conserved 172-amino-acid polypeptide found in the cytosol, nucleus, mitochondria, extracellular space, and circulating blood. It is a multifunctional protein that protects cells against apoptosis, promotes cell growth and cell cycle progression, binds calcium (Ca(2+)) and has antipathogen activities. Its role in the pathogenesis of human and animal diseases is also diverse. Fortilin facilitates the development of atherosclerosis, contributes to both systemic and pulmonary arterial hypertension, participates in the development of cancers, and worsens diabetic nephropathy...
2017: Advances in Clinical Chemistry
https://www.readbyqxmd.com/read/28935602/dietary-immunogen-%C3%A2-modulated-digestive-enzyme-activity-and-immune-gene-expression-in-litopenaeus-vannamei-post-larvae
#12
Hamed Kolangi Miandare, Ali Taheri Mirghaed, Marjan Hosseini, Nastaran Mazloumi, Ashkan Zargar, Sajad Nazari
Pacific white shrimp Litopenaeus vannamei (Boone, 1931) is an important economical shrimp species worldwide, especially in the Middle East region, and farming activities of this species have been largely affected by diseases, mostly viral and bacterial diseases. Scientists have started to use prebiotics for bolstering the immune status of the animal. This study aimed to investigate the influence of Immunogen(®) on growth, digestive enzyme activity and immune related gene expression of Litopenaeus vannamei post-larvae...
September 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28915415/transcriptomic-analysis-of-the-hepatopancreas-induced-by-eyestalk-ablation-in-shrimp-litopenaeus-vannamei
#13
Ji-Hyun Lee, Indyaswan T Suryaningtyas, Tae-Ho Yoon, Jeong Min Shim, Hyun Park, Hyun-Woo Kim
Although eyestalk ablation (ESA) is currently considered the most effective method to facilitate molting and maturation, its physiological responses are still not clearly explained in decapod crustaceans. In this study, we analyzed the hepatopancreatic transcriptomes of Litopenaeus vannamei after ESA using the Illumina Miseq platform. After screening 53,029 contigs with high cutoff values (fold change>|10|; P-value<0.05; RPKM>1), we were able to identify 105 differentially expressed genes (DEGs), of which 100 were up-regulated and five were down-regulated...
September 8, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28889014/transcriptomic-and-morphological-analyses-of-litopenaeus-vannamei-intestinal-barrier-in-response-to-vibrio-paraheamolyticus-infection-reveals-immune-response-signatures-and-structural-disruption
#14
Cancan Qi, Lei Wang, Mei Liu, Keyong Jiang, Mengqiang Wang, Wei Zhao, Baojie Wang
The white shrimp Litopenaeus vannamei has been greatly impacted by Vibrio infection. In this study, we investigated the intestinal barrier response of L vannamei following challenge with Vibrio parahaemolyticus E1, by examining morphological changes and transcriptome expression levels. A total of 16,4420 unigenes were obtained from RNAseq data after quality control and assembly, and 4646 differentially expressed genes (DEGs) were identified following Vibrio challenge, of which 2469 unigenes were significantly up-regulated and 2177 were significantly down-regulated...
September 6, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28844966/lipopolysaccharide-specific-binding-c-type-lectin-with-one-crd-domain-from-fenneropenaeus-merguiensis-fmlc4-functions-as-a-pattern-recognition-receptor-in-shrimp-innate-immunity
#15
Prapaporn Utarabhand, Supattra Thepnarong, Phanthipha Runsaeng
In crustaceans, an innate immune system is solely required because they lack an adaptive immunity. One kind of pattern recognition receptors (PRRs) that plays a particular role in the innate immunity of aquatic shrimp is lectin. A new diverse C-type lectin (FmLC4) was cloned from the hepatopancreas of Fenneropenaeus merguiensis by using RT-PCR and 5' and 3' rapid amplification of cDNA ends approaches. A full-length FmLC4 cDNA comprises 706 bp with an open reading frame of 552 bp, encoding a peptide of 184 amino acids...
August 24, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28842372/various-cellular-responses-of-different-shrimp-haemocyte-subpopulations-to-lipopolysaccharide-stimulation
#16
Jian-An Xian, Xiu-Xia Zhang, Dong-Mei Wang, Jun-Tao Li, Pei-Hua Zheng, Yao-Peng Lu
Different haemocyte types have been reported to play diverse roles in immune defense of shrimp. To investigate the roles of the three haemocyte types [hyaline cells (HC), semigranular cells (SGC) and granular cells (GC)] of shrimp in immune responses against lipopolysaccharide (LPS), percentage, non-specific esterase activity (EA), reactive oxygen species (ROS) production and nitric oxide (NO) production of the three haemocyte subpopulations were analyzed in LPS-injected Penaeus monodon using flow cytometry...
August 24, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28625840/survival-of-litopenaeus-vannamei-shrimp-fed-on-diets-supplemented-with-dunaliella-sp-is-improved-after-challenges-by-vibrio-parahaemolyticus
#17
Diana Medina Félix, José Antonio López Elías, Ángel Isidro Campa Córdova, Luis Rafael Martínez Córdova, Antonio Luna González, Edilmar Cortes Jacinto, Nolberta Huerta Aldaz, Fernando Cano Mendoza, María Guadalupe Burboa Zazueta
Survival of Litopenaeus vannamei was evaluated during a Vibrio parahaemolyticus infection. This bacteria has been causing significant economic losses in the shrimp industry due to the appearance of early mortality syndrome (EMS), also known as acute hepatopancreatic necrosis disease (AHPND). Control of bacteria in ponds is difficult to achieve with antibiotics due to environmental infections and antibiotic resistance. New methods have been proposed to control and prevent the impact of bacterial infections. The physiological response indicated by plasma biochemical parameters in shrimp can determine their health and stress status...
September 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28522419/effects-of-stocking-density-of-the-white-shrimp-litopenaeus-vannamei-boone-on-immunities-antioxidant-status-and-resistance-against-vibrio-harveyi-in-a-biofloc-system
#18
Gang Liu, Songming Zhu, Dezhao Liu, Xishan Guo, Zhangying Ye
Determining optimum stocking density of the white shrimp Litopenaeus vannamei (Boone) is a big concern for shrimp farmers. However, few studies have assessed the influence of stocking density on the antioxidant status, immunology, digestive enzyme activities, and growth performance of white shrimp in biofloc systems. In this study, these parameters of white shrimp in a biofloc system were compared at three stocking densities: 300 orgs m(-3) as low stocking density (LD), 400 orgs m(-3) as medium stocking density (MD), and 500 orgs m(-3) as high stocking density (HD)...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28512288/lactic-acid-bacteria-specific-induction-of-cd4-foxp3-t-cells-ameliorates-shrimp-tropomyosin-induced-allergic-response-in-mice-via-suppression-of-mtor-signaling
#19
Linglin Fu, Jixiang Peng, Shushu Zhao, Yan Zhang, Xiurong Su, Yanbo Wang
The beneficial effects of probiotics have been described in allergic sensitization and diseases; however, many questions remain unanswered, such as characteristics of the most effective strains in modulation of allergic responses and how orally administered probiotics affect the systemic immune system. In the present work, oral administration of five lactic acid bacteria strains showed variable effects on protection against the allergic reaction in a mouse model of food allergy to shrimp tropomyosin (ST). The most effective anti-allergic strain, Bacillus coagulans 09...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28501515/shrimp-humoral-responses-against-pathogens-antimicrobial-peptides-and-melanization
#20
Anchalee Tassanakajon, Vichien Rimphanitchayakit, Suwattana Visetnan, Piti Amparyup, Kunlaya Somboonwiwat, Walaiporn Charoensapsri, Sureerat Tang
Diseases have caused tremendous economic losses and become the major problem threatening the sustainable development of shrimp aquaculture. The knowledge of host defense mechanisms against invading pathogens is essential for the implementation of efficient strategies to prevent disease outbreaks. Like other invertebrates, shrimp rely on the innate immune system to defend themselves against a range of microbes by recognizing and destroying them through cellular and humoral immune responses. Detection of microbial pathogens triggers the signal transduction pathways including the NF-κB signaling, Toll and Imd pathways, resulting in the activation of genes involved in host defense responses...
May 10, 2017: Developmental and Comparative Immunology
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