Shengjiang Liu, Jinzhong Li, Sameera Peraramelli, Ningguang Luo, Alan Chen, Minghua Dai, Fang Liu, Yanbao Yu, Ryan D Leib, Ying Li, Kevin Lin, Derrick Huynh, Shuyi Li, Li Ou
Recombinant adeno-associated (rAAV) vectors could be manufactured by plasmid transfection into human HEK293 cells or baculovirus infection of Spodoptera frugiperda (Sf9) insect cells. However, systematic comparisons between these systems using large-scale, high-quality AAV vectors are lacking. rAAV from Sf9 cells (Sf9-rAAV) at 2-50 L and HEK293 cells (HEK-rAAV) at 2-200 L scales were characterized. HEK-rAAV had ∼40-fold lower yields but ∼10-fold more host cell DNA measured by ddPCR and NGS, respectively...
November 20, 2023: Molecular Therapy