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Recombinant baculovirus

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https://www.readbyqxmd.com/read/29327672/linear-epitopes-in-african-swine-fever-virus-p72-recognized-by-monoclonal-antibodies-prepared-against-baculovirus-expressed-antigen
#1
Mallory E Heimerman, Maria V Murgia, Ping Wu, Andre D Lowe, Wei Jia, Raymond R Rowland
Protein p72 is the major capsid protein of African swine fever virus (ASFV) and is an important target for test and vaccine development. Monoclonal antibodies (mAbs) were prepared against a recombinant antigenic fragment, from amino acid (aa) 20-303, expressed in baculovirus. A total of 29 mAbs were recovered and tested by immunofluorescent antibody (IFA) staining on ASFV Lisbon-infected Vero cells. Six antibodies were IFA-positive and selected for further characterization. Epitope mapping was performed against overlapping polypeptides expressed in E...
January 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29323419/evaluation-of-viral-contamination-in-a-baculovirus-expression-system
#2
Chikako Ono, Junki Hirano, Toru Okamoto, Yoshiharu Matsuura
Insect expression systems based on baculovirus are widely used for the generation of recombinant proteins. Here, we evaluated the infectivity of baculoviruses under the physiological stresses of 'freeze- thaw' and sonication, and the baculoviral contamination of recombinant proteins after protein purification. Our findings suggest that Nonidet™ P-40 (NP-40) treatment of baculoviruses completely abolishes their infectivity and that recombinant proteins purified with affinity beads do not include infectious baculoviruses...
January 11, 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/29320757/expression-of-hneuritn-protein-in-a-baculovirus-expression-system-and-the-analysis-of-its-activity
#3
Shuai Zhang, Yanhong Huang, Jingling Zhu, Liya Shan, Jianfeng Gao, Yunhua Zhang, Na Yu, Lei Yang, Jin Huang
Neuritin plays an important role in the development and regeneration of the nervous system, and shows good prospects in the treatment and protection of the nervous system. To characterize neuritin function, we constructed a baculovirus expression system of neuritin, and identified the biological activity of the neuritin protein. The results and showed that the expression product could promote the neurite growth of dorsal root ganglion in chicken embryos. The neuritin open reading frame was amplified and cloned into the plasmid pFastBac™HTA...
January 7, 2018: Gene
https://www.readbyqxmd.com/read/29300162/production-of-gp64-free-virus-like-particles-from-baculovirus-infected-insect-cells
#4
Lorena C S Chaves, Bergmann M Ribeiro, Gary W Blissard
The retroviral Gag protein is frequently used to generate 'virus-like particles' (VLPs) for a variety of applications. Retroviral Gag proteins self-assemble and bud at the plasma membrane to form enveloped VLPs that resemble natural retrovirus virions, but contain no viral genome. The baculovirus expression vector system has been used to express high levels of the retroviral Gag protein to produce VLPs. However, VLP preparations produced from baculovirus-infected insect cells typically contain relatively large concentrations of baculovirus budded virus (BV) particles, which are similar in size and density to VLPs, and thus may be difficult to separate when purifying VLPs...
January 4, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29295756/a-virus-like-particle-vaccine-confers-protection-against-enterovirus-d68-lethal-challenge-in-mice
#5
Wenlong Dai, Chao Zhang, Xueyang Zhang, Pei Xiong, Qingwei Liu, Sitang Gong, Lanlan Geng, Dongming Zhou, Zhong Huang
Enterovirus D68 (EV-D68) is increasingly associated with severe acute respiratory infection and acute flaccid myelitis (AFM) in children around the world. However, neither vaccines nor therapeutic drugs are available for EV-D68. Here we report the development of a virus-like particle (VLP) based experimental EV-D68 vaccine. We found that EV-D68 VLPs could be successfully generated in insect cells infected with a recombinant baculovirus co-expressing the P1 precursor and 3CD protease of EV-D68. Biochemical and electron microscopic analyses revealed that EV-D68 VLPs were composed of VP0, VP1, and VP3 capsid proteins derived from precursor P1 and were visualized as spherical particles of ∼30 nm in diameter...
December 30, 2017: Vaccine
https://www.readbyqxmd.com/read/29287303/high-levels-of-dek-autoantibodies-in-sera-of-polyarticular-jia-patients-and-in-early-flare-following-cessation-of-anti-tnf-therapy
#6
Nirit Mor-Vaknin, Miguel Rivas, Maureen Legendre, Smriti Mohan, Ye Yuanfan, Theresa Mau, Anne Johnson, Bin Huang, Lili Zhao, Yukiko Kimura, Steven J Spalding, Paula W Morris, Beth S Gottlieb, Karen Onel, Judyann C Olson, Barbara S Edelheit, Michael Shishov, Lawrence K Jung, Elaine A Cassidy, Sampath Prahalad, Murray H Passo, Timothy Beukelman, Jay Mehta, Edward H Giannini, Barbara S Adams, Daniel J Lovell, David M Markovitz
OBJECTIVE: The nuclear oncoprotein DEK is an autoantigen associated with juvenile idiopathic arthritis (JIA), especially the oligoarticular subtype. DEK is a secreted chemoatactic factor. Abundant DEK and DEK autoantibodies are found in the inflamed JIA synovium. In screening sera samples from two different JIA patient cohorts for DEK autoantibodies, we now further characterize the nature of DEK autoantibodies. METHODS: DEK autoantibody levels were analyzed in sera from 33 JIA patients, 13 patients with other inflammatory conditions, and 11 healthy controls and 89 sera samples from JIA patients undergoing anti-TNF therapy...
December 29, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29285311/gene-delivery-and-gene-expression-in-vertebrate-using-baculovirus-bombyx-mori-nucleopolyhedrovirus-vector
#7
Xingjian Liu, Yinü Li, Xiaoyuan Hu, Yongzhu Yi, Zhifang Zhang
The baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) has been investigated as a possible tool for gene therapy, but its inhibition by complement proteins in human serum limits its applicability. Here, we used the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) to construct a gene delivery vector in which a reporter gene is driven by a cytomegalovirus IE promoter. Enhanced green fluorescent protein (EGFP) and luciferase reporter genes were used to test the efficiency of gene delivery...
December 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/29247249/baculovirus-assisted-reovirus-infection-in-monolayer-and-spheroid-cultures-of-glioma-cells
#8
Iris J C Dautzenberg, Sanne K van den Hengel, Jeroen de Vrij, Lars Ravesloot, Steve J Cramer, Saw-See Hong, Diana J M van den Wollenberg, Pierre Boulanger, Rob C Hoeben
The mammalian orthoreovirus Type 3 Dearing has great potential as oncolytic agent in cancer therapy. One of the bottlenecks that hampers its antitumour efficacy in vivo is the limited tumour-cell infection and intratumoural distribution. This necessitates strategies to improve tumour penetration. In this study we employ the baculovirus Autographa californica multiple nucleopolyhedrovirus as a tool to expand the reovirus' tropism and to improve its spread in three-dimensional tumour-cell spheroids. We generated a recombinant baculovirus expressing the cellular receptor for reovirus, the Junction Adhesion Molecule-A, on its envelope...
December 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29238904/anti-inflammatory-activity-of-anti-hyperlipidemic-drug-fenofibrate-and-its-phase-i-metabolite-fenofibric-acid-in-silico-in-vitro-and-in-vivo-studies
#9
G Shyam Prasad, P Govardhan, G Deepika, V Vakdevi, R B Sashidhar
Fenofibrate, an anti-hyperlipidemic drug and its phase-I biotransformed metabolite fenofibric acid, was studied for COX-1 (PDB ID: 3N8Y) and COX-2 (PDB ID: 1PXX) inhibition potentials in silico and in vitro for their effects on human recombinant COX-2 enzyme isolated from a Baculovirus expression system in sf21 cells (EC 1.14.99.1) using a conventional spectrophotometric assay. Furthermore, the compounds were also screened for their anti-inflammatory potentials in vivo using carrageenan-induced paw oedema method in Wistar rats...
December 13, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/29236287/characterization-of-a-norovirus-specific-monoclonal-antibody-that-exhibits-wide-spectrum-binding-activities
#10
Lijun Zheng, Wenhui Wang, Jinjin Liu, Xuhui Chen, Sanjing Li, Qiaoli Wang, Yuqi Huo, Chuan Qin, Shuo Shen, Mingchen Wang
Noroviruses (NoVs) are increasingly recognized as the leading cause of acute non-bacterial gastroenteritis worldwide. To screen for NoV-specific monoclonal antibodies (mAbs) with wide spectrum binding activities that could be used for the development of NoV-related detection reagents, we immunized mice with a combination of virus like particles (VLPs) derived from eight different genotypes (two from genogroup I and six from genogroup II), of which two (GI.7 and GII.2) were newly produced VLPs. Indirect enzyme-linked immunosorbent assay (ELISA) confirmed that two mAbs (8D8 and 10B11) bound to all eight major capsid proteins (VP1) with varied binding abilities...
December 13, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29230222/single-dose-of-consensus-hemagglutinin-based-virus-like-particles-vaccine-protects-chickens-against-divergent-h5-subtype-influenza-viruses
#11
Peipei Wu, Jihu Lu, Xuehua Zhang, Mei Mei, Lei Feng, Daxin Peng, Jibo Hou, Sang-Moo Kang, Xiufan Liu, Yinghua Tang
The H5 subtype highly pathogenic avian influenza (HPAI) virus is one of the greatest threats to global poultry industry. To develop broadly protective H5 subunit vaccine, a recombinant consensus HA sequence (rHA) was constructed and expressed in virus-like particles (rHA VLPs) in the baculovirus-insect cell system. The efficacy of the rHA VLPs vaccine with or without immunopotentiator (CVCVA5) was assessed in chickens. Compared to the commercial Re6 or Re6-CVCVA5 vaccines, single dose immunization of chickens with rHA VLPs or rHA-CVCVA5 vaccines induced higher levels of serum hemagglutinin inhibition titers and neutralization titers, mucosal antibodies, IFN-γ and IL-4 cytokines in sera, and cytotoxic T lymphocyte responses...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29227884/corrigendum-to-characterization-of-an-sf-rhabdovirus-negative-s-frugiperda-cell-line-as-an-alternative-host-for-recombinant-protein-production-in-the-baculovirus-insect-cell-system-protein-expr-purif-122-2016-45-55
#12
Ajay B Maghodia, Christoph Geisler, Donald L Jarvis
No abstract text is available yet for this article.
December 8, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29204900/molecular-design-for-recombinant-adeno-associated-virus-raav-vector-production
#13
REVIEW
Juan Jose Aponte-Ubillus, Daniel Barajas, Joseph Peltier, Cameron Bardliving, Parviz Shamlou, Daniel Gold
Recombinant adeno-associated virus (rAAV) vectors are increasingly popular tools for gene therapy applications. Their non-pathogenic status, low inflammatory potential, availability of viral serotypes with different tissue tropisms, and prospective long-lasting gene expression are important attributes that make rAAVs safe and efficient therapeutic options. Over the last three decades, several groups have engineered recombinant AAV-producing platforms, yielding high titers of transducing vector particles. Current specific productivity yields from different platforms range from 103 to 105 vector genomes (vg) per cell, and there is an ongoing effort to improve vector yields in order to satisfy high product demands required for clinical trials and future commercialization...
December 4, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29203135/exploring-three-different-expression-systems-for-recombinant-expression-of-globins-escherichia-coli-pichia-pastoris-and-spodoptera-frugiperda
#14
An Bracke, David Hoogewijs, Sylvia Dewilde
Globins are among the best investigated proteins in biological and medical sciences and represent a prime tool for the study of the evolution of genes and the structure-function relationship of proteins. Here, we explore the recombinant expression of globins in three different expression systems: Escherichia coli, Pichia pastoris and the baculovirus infected Spodoptera frugiperda. We expressed two different human globin types in these three expression systems: I) the well-characterized neuroglobin and II) the uncharacterized, circular permutated globin domain of the large chimeric globin androglobin...
December 1, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29177536/novel-biotechnological-platform-based-on-baculovirus-occlusion-bodies-carrying-babesia-bovis-small-antigenic-peptides-for-the-design-of-a-diagnostic-enzyme-linked-immunosorbent-assay-elisa
#15
M G López, H M Pallarés, V Alfonso, S J Carmona, M Farber, O Taboga, S E Wilkowsky
Baculoviruses are large DNA virus of insects principally employed in recombinant protein expression. Its ability to form occlusion bodies (OBs), which are composed mainly of polyhedrin protein (POLH), makes them biotechnologically attractive, as these crystals (polyhedra) can incorporate foreign peptides and can be easily isolated. On the other hand, peptide microarrays allow rapid and inexpensive high-throughput serological screening of new candidates to be incorporated to OBs. To integrate these 2 biotechnological approaches, we worked on Babesia bovis, one of the causative agents of bovine babesiosis...
November 27, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29145860/a-new-single-step-protocol-for-rapid-baculovirus-driven-protein-production-in-insect-cells
#16
Judith Scholz, Sabine Suppmann
BACKGROUND: In the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies. Despite the significant improvements introduced during the past years, the BEV system still has major drawbacks, primarily the time required to generate recombinant virus and virus instability for certain target proteins. In this study we show that the conventional method to generate recombinant Baculovirus using a Tn7 transposition based system can be shortened to a single-step transfection-only procedure without further amplification...
November 16, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/29133148/adventitious-viruses-in-insect-cell-lines-used-for-recombinant-protein-expression
#17
REVIEW
Christoph Geisler, Donald L Jarvis
Insect cells are widely used for recombinant protein expression, typically as hosts for recombinant baculovirus vectors, but also for plasmid-mediated transient transfection or stable genetic transformation. Insect cells are used to express proteins for research, as well as to manufacture biologicals for human and veterinary medicine. Recently, several insect cell lines used for recombinant protein expression were found to be persistently infected with adventitious viruses. This has raised questions about how these infections might affect research performed using those cell lines...
November 10, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29121909/successful-production-of-the-potato-antimicrobial-peptide-snakin-1-in-baculovirus-infected-insect-cells-and-development-of-specific-antibodies
#18
Natalia Inés Almasia, María Paula Molinari, Guillermo Andrés Maroniche, Vanesa Nahirñak, María Pilar Barrios Barón, Oscar Alberto Taboga, Cecilia Vazquez Rovere
BACKGROUND: Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recognized since it exhibits in vivo antifungal and antibacterial activity. Most attempts to produce StSN1, or homologous peptides, in a soluble native state using bacterial, yeast or synthetic expression systems have presented production bottlenecks such as insolubility, misfolding or low yields. RESULTS: In this work, we successfully expressed a recombinant StSN1 (rSN1) in Spodoptera frugiperda (Sf9) insect cells by optimizing several of the parameters for its expression in the baculovirus expression system...
November 9, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/29119112/expression-and-immunogenicity-of-two-recombinant-fusion-proteins-comprising-foot-and-mouth-disease-virus-structural-protein-vp1-and-dc-sign-binding-glycoproteins
#19
Xinsheng Liu, Jianliang Lv, Yuzhen Fang, Peng Zhou, Yanzhen Lu, Li Pan, Zhongwang Zhang, Junwu Ma, Yongguang Zhang, Yonglu Wang
Improving vaccine immunogenicity by targeting antigens to dendritic cells has recently emerged as a new design strategy in vaccine development. In this study, the VP1 gene of foot-and-mouth disease virus (FMDV) serotype A was fused with the gene encoding human immunodeficiency virus (HIV) membrane glycoprotein gp120 or C2-V3 domain of hepatitis C virus (HCV) envelope glycoprotein E2, both of which are DC-SIGN-binding glycoproteins. After codon optimization, the VP1 protein and the two recombinant VP1-gp120 and VP1-E2 fusion proteins were expressed in Sf9 insect cells using the insect cell-baculovirus expression system...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29115972/plasmodium-reichenowi-eba-140-merozoite-ligand-binds-to-glycophorin-d-on-chimpanzee-red-blood-cells-shedding-new-light-on-origins-of-plasmodium-falciparum
#20
Agata Zerka, Radoslaw Kaczmarek, Marcin Czerwinski, Ewa Jaskiewicz
BACKGROUND: All symptoms of malaria are caused by the intraerythrocytic proliferation of Plasmodium merozoites. Merozoites invade erythrocytes using multiple binding ligands that recognise specific surface receptors. It has been suggested that adaptation of Plasmodium parasites to infect specific hosts is driven by changes in genes encoding Plasmodium erythrocyte-binding ligands (EBL) and reticulocyte-binding ligands (RBL). Homologs of both EBL and RBL, including the EBA-140 merozoite ligand, have been identified in P...
November 7, 2017: Parasites & Vectors
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