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https://www.readbyqxmd.com/read/29334260/crude-extract-of-origanum-vulgare-l-induced-cell-death-and-suppressed-mapk-and-pi3-akt-signaling-pathways-in-sw13-and-h295r-cell-lines
#1
Beatrice Rubin, Jacopo Mansi, Halenya Monticelli, Loris Bertazza, Marco Redaelli, Francesca Sensi, Maira Zorzan, Carla Scaroni, Caterina Mian, Maurizio Iacobone, Decio Armanini, Cristina Bertolini, Susi Barollo, Marco Boscaro, Raffaele Pezzani
Oregano (Origanum vulgare L.) is a common aromatic plant used in Mediterranean and Asian Regions for treating respiratory diseases, painful menstruation, rheumatoid arthritis, etc. Recently its role as an anticancer plant has been suggested, although oregano has been never evaluated into adrenocortical tumour cell models. This study analysed for the first time the anticancer effects of a crude extract of wild mountain oregano (Origanum vulgare L.) in SW13 and H295R cell lines. The crude extract was characterised by GC/MS and the toxic effects of oregano were first analysed by brine shrimp lethality assay...
January 15, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29331315/ethnobotany-phytochemistry-and-pharmacology-of-arctotis-arctotoides-l-f-o-hoffm-a-review
#2
REVIEW
Md Moshfekus Saleh-E-In, Johannes Van Staden
ETHNOPHARMACOLOGICAL RELEVANCE: Arctotis arctotoides (Asteraceae) is part of the genus Arctotis. Arctotis is an African genus of approximately 70 species that occur widely in the African continent with diverse medicinal values. This plant is used for the treatment of indigestion and catarrh of the stomach, epilepsy, topical wounds and skin disorders among the ethnic groups in South Africa and reported to have a wide spectrum of pharmacological properties. AIM OF THE REVIEW: The aim of the present review is to appraise the botany, traditional uses, phytochemistry, pharmacological potential, analytical methods and safety issues of A...
January 10, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29329856/effect-of-tyrosinase-aided-crosslinking-on-the-ige-binding-potential-and-conformational-structure-of-shrimp-metapenaeus-ensis-tropomyosin
#3
Ishfaq Ahmed, Liangtao Lv, Hong Lin, Zhenxing Li, Jiaju Ma, Chen Guanzhi, Lirui Sun, Lili Xu
The present study was performed to determine crosslinking and oxidative reactions catalyzed by tyrosinase (Tyr), caffeic acid (CA) and their combination with respect to IgE binding potential and conformational structure of shrimp tropomyosin (TM). Cross-links and IgE binding potentials were analyzed by SDS-PAGE, western blot and indirect ELISA. While structural changes were characterized using surface hydrophobicity, ultraviolet (UV), fluorescence and circular dichroism (CD) spectroscopies. Maximum reduction in the IgG (37...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29325710/wnt5b-regulates-apoptosis-in-litopenaeus-vannamei-against-white-spot-syndrome-virus
#4
Chuanqi Wang, Lingwei Ruan, Hong Shi, Xun Xu
The Wnt signaling mediated by Wnt proteins that orchestrate and influence a myriad of cellular processes, such as cell proliferation, differentiation, tumorigenesis, apoptosis, and participation in immune defense during microbe infection. Wnt5b is one of the Wnt signaling molecules that initiate the cascade. In this study, we cloned and characterized a Wnt5b homolog from Litopenaeus vannamei designed as LvWnt5b. The full length of LvWnt5b transcript was 1726 bp with an 1107 bp open reading frame that encoded a 368 aa protein, which contained 24 discontinuous and highly conserved cysteine...
January 8, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29322345/freshwater-shrimps-macrobrachium-depressimanum-and-macrobrachium-jelskii-as-biomonitors-of-hg-availability-in-the-madeira-river-basin-western-amazon
#5
R C F Galvão, I B B Holanda, D P De Carvalho, R Almeida, C M M Souza, L D Lacerda, W R Bastos
Total mercury (THg) concentrations measured in two freshwater shrimp species (Macrobrachium depressimanum and Macrobrachium jelskii) showed a relationship with the location of artisanal and small-scale gold mining (ASGM) from the Madeira River Basin, Western Amazon. Between August 2009 and May 2010, 212 shrimp samples were collected in the confluence of the Madeira River with three of its tributaries (Western Amazon). THg concentration was quantified in the exoskeleton, hepatopancreas and muscle tissue of the shrimps by cold vapor atomic absorption spectrophotometry...
January 10, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29322096/dystrophin-gene-expression-and-intracellular-calcium-changes-in-the-giant-freshwater-prawn-macrobrachium-rosenbergii-in-response-to-white-spot-symptom-disease-infection
#6
Anees Fathima Noor, Tze Chiew Christie Soo, Farhana Mohd Ghani, Zee Hong Goh, Li Teng Khoo, Subha Bhassu
Background: Dystrophin, an essential protein functional in the maintenance of muscle structural integrity is known to be responsible for muscle deterioration during white spot syndrome virus (WSSV) infection among prawn species. Previous studies have shown the upregulation of dystrophin protein in Macrobrachium rosenbergii (the giant freshwater prawn) upon white spot syndrome virus (WSSV) infection. The literature has also suggested the important role of calcium ion alterations in causing such muscle diseases...
December 2017: Heliyon
https://www.readbyqxmd.com/read/29322058/gas-chromatography-gc-mass-analysis-and-bioactivity-of-essential-oil-from-aerial-parts-of-ferulago-trifida-antimicrobial-antioxidant-ache-inhibitory-general-toxicity-mtt-assay-and-larvicidal-activities
#7
Saeed Tavakoli, Hassan Vatandoost, Reza Zeidabadinezhad, Reza Hajiaghaee, Abbas Hadjiakhoondi, Mohammad Reza Abai, Narguess Yassa
Background: We aimed to investigate different biological properties of aerial parts essential oil of Ferulago trifida Boiss and larvicidal activity of its volatile oils from all parts of plant. Methods: Essential oil was prepared by steam distillation and analyzed by Gas chromatography and GC/Mass. Antioxidant, antimicrobial, cytotoxic effects and AChE inhibitory of the oil were investigated using DPPH, disk diffusion method, MTT assay and Ellman methods. Larvicidal activity of F...
September 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/29320367/antimicrobial-and-antioxidant-activities-of-two-polyketides-from-lichen-endophytic-fungus-preussia-sp
#8
Babita Paudel, Keshab Bhattarai, Hari Datta Bhattarai
Two compounds, compounds 1 and 2, were isolated from Preussia sp. The molecular structures of both compounds were elucidated by analyzing one-dimensional (1D) and 2D nuclear magnetic resonance data along with high-resolution mass spectrometry data. Compound 1 was obtained as novel in structure, and compound 2 was recently reported elsewhere. Compound 1 did not show antioxidant and antimicrobial activities and brine shrimp toxicity, while compound 2 showed strong antioxidant activity (DPPH reduction capacity; IC50=3 μg/mL) and brine shrimp toxicity (LD50=50 μg/mL)...
January 10, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/29319184/diagnostic-accuracy-risk-assessment-and-cost-effectiveness-of-component-resolved-diagnostics-for-food-allergy-a-systematic-review
#9
REVIEW
Javier Flores Kim, Nicola McCleary, Bright I Nwaru, Andrew Stoddart, Aziz Sheikh
BACKGROUND: Component-resolved diagnostics (CRD) are promising tools for diagnosing food allergy, offering the potential to determine specific phenotypes and to develop patient-tailored risk profiles. Nevertheless, the diagnostic accuracy of these tests varies across studies; thus, their clinical utility remains unclear. Therefore, we synthesized the evidence from studies investigating the diagnostic accuracy, risk assessment ability, and cost-effectiveness of CRD for food allergy. METHODS: We systematically searched 10 electronic databases and four clinical trial registries for studies published January 2000-February 2017...
January 10, 2018: Allergy
https://www.readbyqxmd.com/read/29314726/the-complete-genome-sequence-of-vibrio-aestuarianus-w-40-reveals-virulence-factor-genes
#10
Xiaojin Xu, Lixing Huang, Yongquan Su, Qingpi Yan
Vibrio aestuarianus is an opportunistic environmental pathogen that has been associated with epidemics in cultured shrimp Penaeus vannamei. Hepatopancreas microsporidian (HPM) and monodon slow growth syndrome (MSGS) have been reported in cultured P. vannamei. In this study, we sequenced and assembled the whole genome of V. aestuarianus strain W-40, a strain that was originally isolated from the intestines of an infected P. vannamei. The genome of V. aestuarianus strain W-40 contains two circular chromosomes of 483,7307 bp with a 46...
January 3, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29314269/semi-quantitative-dot-blot-immunogold-assay-for-specific-detection-of-white-spot-syndrome-virus-wssv
#11
Nittaya Bunsanong, Wilaiwan Chotigeat, Panchalika Deachamag, Suchera Thananimit
A Dot-blot immunogold assay (DBIA) was developed to detect white spot syndrome virus (WSSV) using the polyclonal antibody VP26 (anti-VP26). The anti-VP26 was immobilized on gold nanoparticles (Ab-AuNPs) and a nitrocellulose membrane was used as a detection pad. When the target WSSV bound to the Ab-AuNPs a reddish dots appeared on the surface of the membrane used within 2 to 5 minutes which could be seen with the naked eye. The test was able to detect WSSV at concentrations as low as 105 copies μl-1 of WSSV...
January 4, 2018: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/29314166/ecotoxicology-of-manufactured-graphene-oxide-nanomaterials-and-derivation-of-preliminary-guideline-values-for-freshwater-environments
#12
Marijana Markovic, Anupama Kumar, Ivan Andjelkovic, Supriya Lath, Jason K Kirby, Dusan Losic, Graeme E Batley, Michael J McLaughlin
The unique physical and chemical properties of graphene-based nanomaterials (GNMs) have inspired a diverse range of scientific and industrial applications. The market value of GNMs is predicted to reach $US 1.3 billion by 2023. Common to many nanomaterials (NMs), an important and unresolved question is the environmental consequences of the increases in GNM use. The current deficiencies in studies reporting ecotoxicology data for GNMs include, differences in analytical methodologies for quantification, no standardized test guidelines, differences in morphology of GNMs, the lack of Chemical Abstract Service (CAS) numbers and the quality of the reported data...
January 4, 2018: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29313524/multichannel-spectrometers-in-animals
#13
Thomas Wells Cronin, Missael Garcia, Viktor Gruev
Multispectral, hyperspectral, polarimetric, and other types of multichannel imaging spectrometers are coming into common use for a variety of applications, including remote sensing, material identification, forensics, and medical diagnosis. These instruments are often bulky and intolerant of field abuse, so designing compact, reliable, portable, and robust devices is a priority. In contrast to most engineering designs, animals have been building compact and robust multichannel imaging systems for millennia - their eyes...
January 9, 2018: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/29305988/stimulating-effects-of-polysaccharide-from-angelica-sinensis-on-the-nonspecific-immunity-of-white-shrimps-litopenaeus-vannamei
#14
Saikun Pan, Longfa Jiang, Shengjun Wu
Angelica sinensis polysaccharide (ASP) was prepared by hot water extraction. Then, high-performance liquid chromatography and ion chromatography analyses were conducted, and the results indicated that ASP is a heteropolysaccharide, has a molecular mass of 82,000 Da and consists of arabinose, galactose and glucose (molar ratio of 6:1:1). The effects of ASP on the nonspecific immunity of white shrimps (Litopenaeus vannamei) were investigated by feeding them with ASP-containing diets (0.5, 1 and 1.5 g/kg) during a 12-week breeding experiment...
January 3, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29305987/l-3-4-dihydroxyphenylalanine-l-dopa-induces-neuroendocrinological-physiological-and-immunological-regulation-in-white-shrimp-litopenaeus-vannamei
#15
Ratchaneegorn Mapanao, Hsin-Wei Kuo, Chin-Chuan Chang, Kuan-Fu Liu, Winton Cheng
L-3,4-Dihydroxyphenylalanine (l-DOPA) is a precursor for dopamine (DA) synthesis. Assessments were conducted to analyze the effects of l-DOPA on mediating regulation of neuroendocrinological, immunological, and physiological parameters in the shrimp, Litopenaeus vannamei when they were individually injected with 0.01 N HCl or l-DOPA at 0.5 or 1.0 μmol shrimp-1 for 60, 120, and 240 min. For catecholamine synthesis evaluation, tyrosine hydroxylase (TH) and DA beta hydroxylase (DBH) activities, l-DOPA, DA, and norepinephrine (NE) levels in hemolymph were determined...
January 3, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29305331/differential-expression-of-micrornas-in-hemocytes-from-white-shrimp-litopenaeus-vannamei-under-copper-stress
#16
Hui Guo, Zhi-Cheng Lu, Xiao-Wen Zhu, Chun-Hua Zhu, Cheng-Gui Wang, Yu-Chun Shen, Wei Wang
MicroRNAs (miRNAs) are small noncoding RNAs that regulate diverse cellular processes, including organismal stress response, through posttranscriptional repression of gene transcripts. They are known to have antiviral functions in aquatic crustacean species, but little is known about the role of miRNAs against environmental stress caused by Cu, a common chemical contaminant in aquatic environment. We performed small RNA sequencing to characterize the differentially expressed microRNAs in Cu exposed shrimp. A total of 4524 known miRNAs and 73 novel miRNAs were significantly (P < ...
January 2, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29303776/evaluation-of-morning-glory-jacquemontia-tamnifolia-l-griseb-leaves-for-antioxidant-antinociceptive-anticoagulant-and-cytotoxic-activities
#17
Mohammad Shahadat Hossain, A S M Ali Reza, Md Masudur Rahaman, Mst Samima Nasrin, Mohammed Rasib Uddin Rahat, Md Rabiul Islam, Md Josim Uddin, Md Atiar Rahman
BACKGROUND: The present study was planned to investigate the phytochemical, antioxidant, antinociceptive, anticoagulant and cytotoxic activities of the Jacquemontia tamnifolia (L.) Griseb leaf methanol extract (MExJT) in the laboratory using both in vitro and in vivo methods. METHODS: Phytochemical values, namely, total phenolic and flavonoid contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect and FeCl3 reducing power effects, were studied by established methods...
January 5, 2018: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/29302889/correction-to-effect-of-biosolid-hydrochar-on-toxicity-to-earthworms-and-brine-shrimp
#18
Tatiane Medeiros Melo, Michael Bottlinger, Elke Schulz, Wilson Mozena Leandro, Adelmo Menezes de Aguiar Filho, Yong Sik Ok, Jörg Rinklebe
Unfortunately, in the original publication of the article, Prof. Yong Sik Ok's affiliation was incorrectly published. The author's affiliation is as follows.
January 4, 2018: Environmental Geochemistry and Health
https://www.readbyqxmd.com/read/29298707/shuang-huang-lian-prevents-basophilic-granulocyte-activation-to-suppress-th2-immunity
#19
Qiaoling Fei, Yixin Han, Ruijuan Qi, Yuan Gao, Lei Fang, Rui Hou, Runlan Cai, Yun Qi
BACKGROUND: Basophilic granulocytes (BGs) not only initiate the induction of Th2 cell differentiation, but also amplify the ongoing Th2 response. Shuang-Huang-Lian (SHL) is clinically used for relieving type I hypersensitivity by continuous treatment for several weeks. METHODS: ELISA, flow cytometry, magnetic activated cell sorting, isoelectric precipitation, hybridoma technique, transfection and luciferase reporter assay were used in this study. The statistical analysis was performed using a one-way ANOVA...
January 3, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29297835/microsporidiosis-in-peppermint-shrimp-decapoda-hippolytidae-lysmata-spp
#20
Elise E B LaDouceur, Brian G Murphy
Microsporidia are a diverse, parasitic phylum closely related to fungi. They infect a broad range of host species and tissues. This report describes microsporidiosis infection in a captive, wild-caught peppermint shrimp ( Lysmata spp.). The animal was found dead in its enclosure approximately 5 days after molting. Grossly, the skeletal musculature was diffusely swollen, opaque, and pale tan to white. Histologically, skeletal myofibers were replaced by large aggregates of sporophorous vesicles containing up to eight spores and earlier stages of sporogony...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
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