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https://www.readbyqxmd.com/read/29149628/multiplex-taqman-locked-nucleic-acid-real-time-pcr-for-the-differential-identification-of-various-meat-and-meat-products
#1
Rusu Xu, Shuang Wei, Guangbiao Zhou, Jiao Ren, Zhongyong Liu, Shuze Tang, Peter C K Cheung, Xiyang Wu
Meat adulteration incidents have been reported frequently over the past few years, and the corresponding traceability issues attracted much more attention due to the customer's demands and administration's responsibility. Therefore, it is important to develop high-throughput and rapid detection methods to identify the specific sources from meat samples. In this study, a multiplex TaqMan locked nucleic acid real-time polymerase chain reaction assay (MLNA-RT-PCR) was developed to simultaneously detect multiple meat sources (duck, pork, beef and chicken)...
November 14, 2017: Meat Science
https://www.readbyqxmd.com/read/29146444/snps-in-the-toll1-receptor-of-litopenaeus-vannamei-are-associated-with-immune-response
#2
Defu Yao, Huimin Su, Jinghua Zhu, Xianliang Zhao, Jude Juventus Aweya, Fan Wang, Mingqi Zhong, Yueling Zhang
Tolls and Toll-like receptors (TLRs) are important regulators in the innate immune system and their genetic variations usually affect the host's susceptibility/resistance to pathogen infections. In this study, we report on the single nucleotide polymorphisms (SNPs) of Toll1 in Litopenaeus vannamei (LvToll1) and how this is associated with immune response. PCR-DGGE analysis revealed genetic polymorphisms in LvToll1 at both the genomic DNA (gDNA) and cDNA levels. Using high-throughput sequencing, 223 SNPs were identified at the gDNA level, of which 145 were non-synonymous SNP (nsSNP), with 3 nsSNPs having frequency over 1%...
November 13, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29145028/the-complete-mitochondrial-genome-of-the-alvinocaridid-shrimp-shinkaicaris-leurokolos-decapoda-caridea-insight-into-the-mitochondrial-genetic-basis-of-deep-sea-hydrothermal-vent-adaptation-in-the-shrimp
#3
Shao'e Sun, Ming Hui, Minxiao Wang, Zhongli Sha
Deep-sea hydrothermal vent is one of the most extreme environments on Earth with low oxygen and high levels of toxins. Decapod species from the family Alvinocarididae have colonized and successfully adapted to this extremely harsh environment. Mitochondria plays a vital role in oxygen usage and energy metabolism, thus it may be under selection in the adaptive evolution of the hydrothermal vent shrimps. In this study, the mitochondrial genome (mitogenome) of alvinocaridid shrimp Shinkaicaris leurokolos (Kikuchi & Hashimoto, 2000) was determined through Illumina sequencing...
November 10, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29142398/cytotoxic-antimitotic-and-antiproliferation-studies-on-rasam-a-south-indian-traditional-functional-food
#4
Agilandeswari Devarajan, M K Mohan Maruga Raja
Background: Rasam is a traditional South Indian food, prepared using tamarind juice as a base, with a variety of spices. Rasam, with all its ingredients medicinally claimed for various ailments, is a functional food. Systematic consumption of traditional functional food provides an excellent preventive measure to ward off many diseases. Objective: To study rasam for cytotoxic, antimitotic, and antiproliferation potential beyond its culinary and nutritional effect...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29142305/assay-interference-and-off-target-liabilities-of-reported-histone-acetyltransferase-inhibitors
#5
Jayme L Dahlin, Kathryn M Nelson, Jessica M Strasser, Dalia Barsyte-Lovejoy, Magdalena M Szewczyk, Shawna Organ, Matthew Cuellar, Gurpreet Singh, Jonathan H Shrimp, Nghi Nguyen, Jordan L Meier, Cheryl H Arrowsmith, Peter J Brown, Jonathan B Baell, Michael A Walters
Many compounds with potentially reactive chemical motifs and poor physicochemical properties are published as selective modulators of biomolecules without sufficient validation and then propagated in the scientific literature as useful chemical probes. Several histone acetyltransferase (HAT) inhibitors with these liabilities are now routinely used to probe epigenetic pathways. We profile the most commonly used HAT inhibitors and confirm that the majority of them are nonselective interference compounds. Most (15 out of 23, 65%) of the inhibitors are flagged by ALARM NMR, an industry-developed counter-screen for promiscuous compounds...
November 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/29141882/transparent-anemone-shrimp-ancylomenes-pedersoni-become-opaque-after-exercise-and-physiological-stress-in-correlation-with-increased-hemolymph-perfusion
#6
Laura E Bagge, Stephen T Kinsey, Justin Gladman, Sönke Johnsen
Whole-body transparency, an effective camouflage strategy in many aquatic species, can be disrupted by environmental and/or physiological stressors. We found that tail-flip escape responses temporarily disrupt the transparency of the anemone shrimp Ancylomenes pedersoni After as few as three tail flips, the previously transparent abdominal muscle became cloudy. Eliciting additional tail flips to the point of exhaustion (16±1 s.e.m.; n=23) resulted in complete opacity, though the original transparency returned after 20-60 min of inactivity...
November 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29134144/composition-diversity-and-function-of-intestinal-microbiota-in-pacific-white-shrimp-litopenaeus-vannamei-at-different-culture-stages
#7
Shenzheng Zeng, Zhijian Huang, Dongwei Hou, Jian Liu, Shaoping Weng, Jianguo He
Intestinal microbiota is an integral component of the host and plays important roles in host health. The pacific white shrimp is one of the most profitable aquaculture species commercialized in the world market with the largest production in shrimp consumption. Many studies revealed that the intestinal microbiota shifted significantly during host development in other aquaculture animals. In the present study, 22 shrimp samples were collected every 15 days from larval stage (15 day post-hatching, dph) to adult stage (75 dph) to investigate the intestinal microbiota at different culture stages by targeting the V4 region of 16S rRNA gene, and the microbial function prediction was conducted by PICRUSt...
2017: PeerJ
https://www.readbyqxmd.com/read/29133276/de-novo-assembly-and-annotation-of-the-whole-genomic-analysis-of-vibrio-campbellii-rt-1-strain-from-infected-shrimp-litopenaeus-vannamei
#8
R Thirugnanasambandam, D Inbakandan, L Stanley Abraham, C Kumar, S M Sundaram, B Subashni, R Vasantharaja, A Arul Anantha Kumar, R Kirubagaran, S Ajmal Khan, T Balasubramanian
OBJECTIVES: This study aimed to sequence the whole genome of Vibrio campbellii RT-1 strain. METHODS: V. campbellii strain was isolated from an infected shrimp, Litopenaeus vannamei collected from aquaculture ponds, India (12.1899° N, 79.9249° E). The whole genome sequencing (WGS) was performed using the Illumina Hiseq 2500 platform and assembled de novo using SPAdes and Velvet optimiser. Furthermore, the gene prediction and annotation were performed by a rapid prokaryotic genome tool-Prokka...
November 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29133253/the-oxidative-stress-and-antioxidant-responses-of-live-chinese-shrimp-fenneropenaeuschinensis-to-low-temperature-and-air-exposure
#9
Zihan Xu, Joe M Regenstein, Dandan Xie, Wenjing Lu, Xingchen Ren, Jiajia Yuan, Linchun Mao
Low temperature and air exposure were the key attributes for waterless transportation of fish and shrimp. In order to investigate the oxidative stress and antioxidant responses of the live shrimp Fenneropenaeuschinensis in the mimic waterless transportation, live Chinese shrimp were cooled at 13 °C for 3 min, stored in oxygen at 15 °C for 12 h, and then revived in water at 25 °C. The survival rate of shrimp under this waterless transportation system was over 86.67%. The ultrastructure of hepatopancreas cells were observed while activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione peroxidase (GSH-Px), antisuperoxide anion free radicals (ASAFR), total antioxidant capacity (TAOC), reactive oxygen species (ROS) production, content of malondialdehyde (MDA) and relative mRNA expressions of CAT and GSH-Px in the hemolymph and hepatopancreas were determined...
November 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29130778/lack-of-induced-mutagenesis-in-e-coli-or-human-lymphoblast-cell-line-upon-long-term-sub-culturing-in-medium-from-irradiated-meat
#10
Sachin N Hajare, Jyoti Tripathi, Satyendra Gautam
PURPOSE: Current study was aimed to enhance the confidence of consumers as well as entrepreneurs towards food irradiation program. MATERIALS AND METHODS: In this work, safety of high dose (25 kGy) irradiated meat samples (HDIMS) was ascertained by scoring mutation frequency through a long-term sub-culturing study in Escherichia coli MG1655 cells (ATCC 700926) up to 1500 generations (at 1%), 250 generations (at 5 and 10%) and human lymphoblast thymidine kinase heterozygote (TK6) cell line (ATCC CRL-8015) [at two gene loci, tk(-/+) (thymidine kinase) and hprt(+) (Hypoxanthine Phosphoribosyltransferase)] up to 156 generations using goat meat sample...
November 13, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/29128494/the-shrimp-hormone-receptor-acts-as-an-anti-apoptosis-and-anti-inflammatory-factor-in-innate-immunity
#11
Zhi Wang, Baozhen Sun, Fei Zhu
Previously, we found that the expression of several genes, including HR, varied in Drosophila melanogaster after white spot syndrome virus (WSSV) infection. In this present study, we further investigated the role of HR in Kuruma shrimp, Marsupenaeus japonicus and determined its anti-apoptosis and anti-inflammation role in the innate immune system. We successfully identified a partial sequence (866 bp in length) of the M. japonicus hormone receptor ligand binding domain (mjHR_LBD/mjHR). The 5' end of mjHR was successfully obtained; the open reading frame (ORF) ran from 33 to 701 bp, and encoded a protein containing 222 amino acids...
November 8, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29127029/extract-from-the-fermented-soybean-product-natto-inhibits-vibrio-biofilm-formation-and-reduces-shrimp-mortality-from-vibrio-harveyi-infection
#12
Pattanan Yatip, D Nitin Chandra Teja, Timothy W Flegel, Chumporn Soowannayan
Many bacteria, including Vibrio pathogens of shrimp, need to colonize and/or form biofilms in hosts or the environment to cause disease. Thus, one possible control strategy for shrimp Vibriosis is biofilm inhibition. With this objective, an extract from the Japanese fermented soybean product, Natto was tested with the luminescent shrimp pathogen Vibrio harveyi (VH) for its ability to inhibit or degrade biofilm and to interfere with cell growth in broth. Natto is a traditional fermentation product of Bacillus subtilis var Natto (BSN1)...
November 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29116290/drug-resistance-ampc-%C3%AE-lactamase-and-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-isolated-from-fish-and-shrimp
#13
Marília Viana Albuquerque de Almeida, Ítalo Mendes Cangussú, Antonia Leonadia Siqueira de Carvalho, Izabelly Linhares Ponte Brito, Renata Albuquerque Costa
The present study aims to detect the production of extended-spectrum beta-lactamases (ESBL) by enterobacteria isolated from samples of fresh shrimp and fish obtained from the retail trade of the city of Sobral, Ceará State, Brazil. All bacterial isolates were submitted to identification and antimicrobial susceptibility testing using aminopenicillin, beta-lactamase inhibitors, carbapenem, 1st, 2nd, 3rd and 4th generation cephalosporins, and monobactam. Three types of beta-lactamases - ESBL, AmpC and KPC - were investigated...
November 6, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/29115743/survivability-of-low-pathogenic-h9n2-avian-influenza-virus-in-water-in-the-presence-of-atyopsis-moluccensis-bamboo-shrimp
#14
A P Pathak, H V Murugkar, S Nagarajan, R Sood, C Tosh, M Kumar, C K Athira, A Praveen
Low pathogenic avian influenza virus (LPAIV) exhibits an ecological climax with the aquatic ecosystem. The most widely prevalent subtype of LPAIV is H9N2. Wild aquatic birds being the natural reservoirs and ducks, the "Trojan horses" for Avian Influenza Virus (AIV), can contaminate the natural water bodies inhabited by them. The virus can persist in the contaminated water from days to years depending upon the environmental conditions. Various aquatic species other than ducks can promote the persistence and transmission of AIV; however, studies on the role of aquatic fauna in persistence and transmission of avian influenza virus are scarce...
November 8, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/29114571/antioxidant-antibacterial-and-cytotoxic-potential-of-silver-nanoparticles-synthesized-using-terpenes-rich-extract-of-lantana-camara-l-leaves
#15
Patil Shriniwas P, Kumbhar Subhash T
Several attempts have been made for green synthesis of silver nanoparticles (AgNPs) using different plant extracts. Present study revealed that, antioxidant, antibacterial and cytotoxic AgNPs were synthesized using terpenes-rich extract (TRE) of environmentally notorious Lantana camara L. leaves. AgNPs were characterized by advanced techniques like UV-Visible and Infra red spectroscopy; XRD, SEM techniques as terpenes coated sphere shaped NPs with average diameter 425 nm. Further, on evaluation, AgNPs were found to exhibit dose - dependent antioxidant potential, good to moderate antibacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa; and toxicity on Brine shrimp (A...
July 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/29112160/reclamation-of-marine-chitinous-materials-for-the-production-of-%C3%AE-glucosidase-inhibitors-via-microbial-conversion
#16
Van Bon Nguyen, San-Lang Wang
Six kinds of chitinous materials have been used as sole carbon/nitrogen (C/N) sources for producing α-glucosidase inhibitors (aGI) by Paenibacillus sp. TKU042. The aGI productivity was found to be highest in the culture supernatants using demineralized crab shell powder (deCSP) and demineralized shrimp shell powder (deSSP) as the C/N source. The half maximal inhibitory concentration (IC50) and maximum aGI activity of fermented deCSP (38 µg/mL, 98%), deSSP (108 µg/mL, 89%), squid pen powder (SPP) (422 µg/mL, 98%), and shrimp head powder (SHP) (455 µg/mL, 92%) were compared with those of fermented nutrient broth (FNB) (81 µg/mL, 93%) and acarbose (1095 µg/mL, 74%), a commercial antidiabetic drug...
November 7, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29109705/complete-genome-sequence-of-vibrio-campbellii-lmb-29-isolated-from-red-drum-with-four-native-megaplasmids
#17
Jinxin Liu, Zhe Zhao, Yiqing Deng, Yan Shi, Yupeng Liu, Chao Wu, Peng Luo, Chaoqun Hu
Vibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limited. The existence of this knowledge gap is at least partially because that V. campbellii was originally classified as Vibrio harveyi, and the detailed information of its comparative genome analysis to other Vibrio spp...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29109224/low-recombination-rates-in-sexual-species-and-sex-asex-transitions
#18
Christoph R Haag, Loukas Theodosiou, Roula Zahab, Thomas Lenormand
In most sexual, diploid eukaryotes, at least one crossover occurs between each pair of homologous chromosomes during meiosis, presumably in order to ensure proper segregation. Well-known exceptions to this rule are species in which one sex does not recombine and specific chromosomes lacking crossover. We review other possible exceptions, including species with chromosome maps of less than 50 cM in one or both sexes. We discuss the idea that low recombination rates may favour sex-asex transitions, or, alternatively may be a consequence of it...
December 19, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/29102705/a-laminin-receptor-like-protein-regulates-white-spot-syndrome-virus-infection-by-binding-to-the-viral-envelope-protein-vp28-in-red-claw-crayfish-cherax-quadricarinatus
#19
Ling-Ke Liu, Wei-Dong Li, Yan Gao, Rong-Yuan Chen, Xiao-Lu Xie, Heng Hong, Ke-Jian Wang, Hai-Peng Liu
White spot syndrome virus (WSSV) is a lethal pathogen of shrimp and many other crustaceans, which has been causing huge economic losses in global aquaculture. Laminin receptor (LR) is a cell surface receptor which participates in the interactions between cells as well as cells and extracellular matrix. Previously, we found that a CqLR-like gene was responsive to WSSV infection in the hematopoietic tissue (Hpt) cells from red claw crayfish Cherax quadricarinatus. To further reveal the role of CqLR-like gene involved in WSSV infection, the full-length cDNA of CqLR-like gene was cloned with 1000 bp, and the open reading frame encoded 308 amino acids with a conserved laminin-binding domain...
November 1, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29102627/the-anti-stresses-capability-of-grp78-in-penaeus-monodon-evidence-from-in-vitro-and-in-vivo-studies
#20
Lili Li, Pengfei Wang, Chao Zhao, Lihua Qiu
Glucose-regulated protein 78 (GRP78) is an important member of the heat shock protein 70 (HSP70) family, which plays important roles in the response to stimulation from various external environmental stimuli. In this study, the full-length cDNA of GRP78 (PmGRP78) was cloned from Penaeus monodon. The qRT-PCR analysis showed that PmGRP78 was ubiquitously expressed in all the tested shrimp tissues, and the expression pattern of PmGRP78 in the gill and hepatopancreas tissues after a pH challenge, osmotic stress, and heavy metal exposure was also detected...
November 2, 2017: Fish & Shellfish Immunology
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