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https://www.readbyqxmd.com/read/28078785/risks-of-ocean-acidification-in-the-california-current-food-web-and-fisheries-ecosystem-model-projections
#1
Kristin N Marshall, Isaac C Kaplan, Emma E Hodgson, Albert Hermann, D Shallin Busch, Paul McElhany, Timothy E Essington, Chris J Harvey, Elizabeth A Fulton
The benefits and ecosystem services that humans derive from the oceans are threatened by numerous global change stressors, one of which is ocean acidification. Here, we describe the effects of ocean acidification on an upwelling system that already experiences inherently low pH conditions, the California Current. We used an end-to-end ecosystem model (Atlantis), forced by downscaled global climate models and informed by a meta-analysis of the pH sensitivities of local taxa, to investigate the direct and indirect effects of future pH on biomass and fisheries revenues...
January 12, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28077637/role-of-litopenaeus-vannamei-yin-yang-1-in-the-regulation-of-the-white-spot-syndrome-virus-immediate-early-gene-ie1
#2
Ping-Han Huang, Ting-Yi Huang, Pei-Si Cai, Li-Kwan Chang
: Yin Yang 1 (YY1) is a multifunctional zinc-finger transcription factor that regulates many key cellular processes. In this study, we report the cloning of YY1 from shrimp, Litopenaeus vannamei, (LvYY1). The study shows that LvYY1 is ubiquitously expressed in shrimp tissues, and knockdown of LvYY1 expression by dsRNA injection in WSSV-infected shrimp reduced both mRNA levels of the WSSV immediate-early gene, ie1, as well as overall copy numbers of the WSSV genome. The cumulative mortality of infected shrimp also declined with LvYY1 dsRNA injection...
January 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28069434/identification-and-functional-analysis-of-a-tep-gene-from-a-crustacean-reveals-its-transcriptional-regulation-mediated-by-nf-%C3%AE%C2%BAb-and-jnk-pathways-and-its-broad-protective-roles-against-multiple-pathogens
#3
Chaozheng Li, Haoyang Li, Bang Xiao, Yonggui Chen, Sheng Wang, Kai Lǚ, Bin Yin, Sedong Li, Jianguo He
Thioester-containing proteins (TEPs) are present in a wide range of species from deuterostomes to protostomes and are thought to be involved in innate immunity. In the current study, a TEP gene homologous to insect TEPs (iTEP) from the crustacean Litopenaeus vannamei, named LvTEP1, is cloned and functionally characterized. The open reading frame (ORF) of LvTEP1 is 4383 bp in length, encoding a polypeptide of 1460 amino acids with a calculated molecular weight of 161.1 kDa LvTEP1, which is most similar to other TEPs from insects, contains some conserved sequence features, including a N-terminal signal peptide, a canonical thioester (TE) motif, and a C-terminal distinctive cysteine signature...
January 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28069369/spatial-and-temporal-distributions-of-hexabromocyclododecanes-in-the-vicinity-of-an-expanded-polystyrene-material-manufacturing-plant-in-tianjin-china
#4
Hongkai Zhu, Kai Zhang, Hongwen Sun, Fei Wang, Yiming Yao
To investigate the environmental fate of 3 main hexabromocyclododecane diastereoisomers (α-, β-, and γ-HBCDs), samples from various environmental media, including outdoor settled dust, soil, sediment, plant tissues (holly, cypress and pine) and marine species (shrimp, crab and fish) were obtained around an expanded polystyrene material manufacturing plant in Tianjin, China. The 3 main HBCD diastereoisomers were detected with the total concentrations ranging from 328 to 31,752 ng/g dry weight (dw), 2.91-1730 ng/g dw, 23...
January 6, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28066712/de-novo-transcriptome-assembly-of-shrimp-palaemon-serratus
#5
Alejandra Perina, Ana M González-Tizón, Iago F Meilán, Andrés Martínez-Lage
: The shrimp Palaemon serratus is a coastal decapod crustacean with a high commercial value. It is harvested for human consumption. In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of adults individuals and, from a pool of larvae. A total number of 4 cDNA libraries were constructed, using the TruSeq RNA Sample Preparation Kit v2. The raw data in this study was deposited in NCBI SRA database with study accession number of SRP090769...
March 2017: Genomics Data
https://www.readbyqxmd.com/read/28065749/characterization-of-haloalkalophilic-organic-solvent-tolerant-protease-for-chitin-extraction-from-shrimp-shell-waste
#6
Thirumalai Maruthiah, Arunachalam Palavesam
Halophilic organic solvent tolerant protease (HOSP) producing Paracoccus saliphilus APCMST-CS5 was isolated from the marine sediment samples and identified through 16S rRNA sequence analysis. P. saliphilus APCMST-CS5 registered maximum HOSP production of 1,321.70U/ml in the medium contained the most significant parameters such as shrimp shell powder (SSP), CaCl2, NaCl, and sardinella powder (SP), obtained through Placket-Burman and Response Surface Methods. HOSP was further purified to 22.68 fold purity with 29...
January 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28065701/recent-progress-toward-the-identification-of-anti-viral-immune-mechanisms-in-decapod-crustaceans
#7
Chris Hauton
The sustainable intensification of crustacean aquaculture, which is dominated by the farming of penaeid shrimp species, continues to be beset by viral disease outbreaks. Despite this, reports exist of differential susceptibility to viral infection between different shrimp species and populations, and between shrimp and other decapod crustaceans. These reports have, in part, provided the motivation to identify key mechanisms of antiviral resistance, or refractivity, in commercially-important species. Within the last decade these studies have created significant advances in our understanding of host virus interactions in decapod models...
January 5, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28062305/involvement-of-second-messengers-in-the-signaling-pathway-of-vitellogenesis-inhibiting-hormone-and-their-effects-on-vitellogenin-mrna-expression-in-the-whiteleg-shrimp-litopenaeus-vannamei
#8
Sun-Hye Bae, Tomoyuki Okutsu, Naoaki Tsutsui, Bong Jung Kang, Hsiang-Yin Chen, Marcy N Wilder
We incubated fragments of Litopenaeus vannamei ovary to investigate second messengers involved in the regulation of vitellogenin (vg) mRNA levels. The use of 100 nM recombinant vitellogenesis-inhibiting hormone (VIH) (corresponding to recombinant L. vannamei sinus gland peptide-G: rLiv-SGP-G) significantly reduced vg mRNA expression in sub-adults after 8 h incubation to less than 20% of the control. The concentration of intracellular cyclic guanosine monophosphate (cGMP) increased 3.2-fold relative to the control after 2 h incubation with rLiv-SGP-G...
January 3, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28062303/purification-and-characterization-of-bioactive-peptides-ryamide-and-cchamide-in-the-kuruma-shrimp-marsupenaeus-japonicus
#9
Tohru Mekata, Tomoya Kono, Jun Satoh, Morikatsu Yoshida, Kenji Mori, Takahiro Sato, Mikiya Miyazato, Takanori Ida
To understand the regulation systems of appetite, bioactive peptides from the kuruma shrimp Marsupenaeus japonicus (Mj) were isolated and purified by reverse pharmacological assays using CHO cells expressing the Drosophila melanogaster G-protein-coupled receptors (GPCRs) CG5811 (a RYamide receptor) or CG14593 (a CCHamide-2 receptor). Four peptides having binding activity to GPCRs were obtained and named Mj RYamide-1, Mj RYamide-2, Mj RYamide-3, and Mj CCHamide. Genes encoding the prepropeptides of these peptides were identified using kuruma shrimp transcriptome databases...
January 4, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28062236/enhanced-wound-healing-activity-of-desert-locust-schistocerca-gregaria-vs-shrimp-penaeus-monodon-chitosan-based-scaffolds
#10
Narguess H Marei, W El-Mazny, Aida El-Shaer, Kareem Dorri Zaki, Zahra S Hussein, Emtithal M Abd-El-Samie
Chitosan (CS) has received great attention in tissue engineering, especially in wound healing acceleration. In this study, chitin was isolated from desert locust (Schistocerca gregaria) and shrimp (Penaeus monodon) then deacetylated to chitosan. Then, chitosan was characterized by degree of deacetylation (DD), molecular weight (M.Wt), swelling index (SI), Fourier transform infrared (FTIR) and X ray diffraction (XRD). The chitosan was then casted into 2D scaffolds and was pictured using scanning electron microscope (SEM)...
January 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28059489/evaluation-of-the-phototoxicity-of-unsubstituted-and-alkylated-pahs-to-mysid-shrimp-americamysis-bahia-validation-of-predictive-models
#11
Bryson E Finch, Solmaz Marzooghi, Dominic M Di Toro, William A Stubblefield
Crude oils are composed of an assortment of hydrocarbons, some of which are polycyclic aromatic hydrocarbons (PAHs). Polycyclic aromatic hydrocarbons are of particular interest due to their narcotic and potential phototoxic effects. Several studies have examined the phototoxicity of individual PAHs and fresh and weathered crude oils and several models have been developed to predict PAH toxicity. Fingerprint analyses of oils have shown that PAHs in crude oils are predominately alkylated. However, current models for estimating PAH phototoxicity assume toxic equivalence between unsubstituted (i...
January 6, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28058488/inhibition-of-staphylococcus-aureus-in-vitro-by-bacteriocinogenic-lactococcus-lactis-kth0-1s-isolated-from-thai-fermented-shrimp-kung-som-and-safety-evaluation
#12
Sutanate Saelao, Suppasil Maneerat, Sireewan Kaewsuwan, Hanitra Rabesona, Yvan Choiset, Thomas Haertlé, Jean-Marc Chobert
Lactococcus lactis KTH0-1S isolated from Thai traditional fermented shrimp (Kung-som) is able to produce heat-stable bacteriocin and inhibits food spoilage bacteria and food-borne pathogens. The inhibitory effect of bacteriocin remained intact after treatment with different pHs and after heating, but was sensitive to some proteolytic enzymes. Addition of bacteriocin KTH0-1S to Staphylococcus aureus cultures decreased viable cell counts by 2.8 log CFU/ml, demonstrating a bactericidal mode of action. Furthermore, the growth of S...
January 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28056950/laboratory-cohabitation-challenge-model-for-shrimp-hepatopancreatic-microsporidiosis-hpm-caused-by-enterocytozoon-hepatopenaei-ehp
#13
Paul Vinu Salachan, Pattana Jaroenlak, Siripong Thitamadee, Ornchuma Itsathitphaisarn, Kallaya Sritunyalucksana
BACKGROUND: Enterocytozoon hepatopenaei (EHP) causes hepatopancreatic microsporidiosis (HPM) in shrimp. It is probably endemic in Australasia and was first characterized and named from the giant or black tiger shrimp Penaeus monodon from Thailand in 2009. Later, it was also found to infect exotic Penaeus vannamei imported for cultivation in Asia. HPM is not normally associated with shrimp mortality, but information from shrimp farmers indicates that it is associated with significant growth retardation that is not clearly noticeable until 2-3 months of cultivation...
January 5, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28054448/taxonomic-research-on-deep-sea-macrofauna-in-the-south-china-sea-using-the-chinese-deep-sea-submersible-jiaolong
#14
REVIEW
Xinzheng Li
This paper reviews the taxonomic and biodiversity studies of the deep-sea invertebrates in the South China Sea based on the samples collected by the Chinese manned deep-sea submersible Jiaolong. To date, six new species were described, including the sponges Lophophysema eversa Gong, Li & Qiu, 2014, Saccocalyx microhexactin Gong, Li & Qiu, 2015, and Semperella jiaolongae Gong, Li & Qiu, 2015 as well as the crustaceans Uroptychus jiaolongae Dong & Li, 2015, Uroptychus spinulosus Dong & Li, 2015 and Globospongicola jiaolongi Jiang, Kou & Li, 2015, and some newly recorded species from the South China Sea were reported...
January 5, 2017: Integrative Zoology
https://www.readbyqxmd.com/read/28053923/prevalence-of-foodborne-pathogens-in-cooked-meat-and-seafood-from-2010-to-2013-in-shandong-province-china
#15
Hua-Ning Zhang, Pei-Bin Hou, Yu-Zhen Chen, Yu Ma, Xin-Peng Li, Hui Lv, Mei Wang, Hai-Lian Tan, Zhen-Wang Bi
BACKGROUND: Current food safety issues are deleteriously reshaping the lifestyle of the population in the developing world. The globalization of food supply impacts patterns of foodborne disease outbreaks worldwide, and consumers are having increased concern about microbiological food safety. METHODS: A total of 2305 samples including sauced meat, sausage, smoked meat, shrimp, sashimi and shellfish were collected from different farmer's markets and supermarkets...
December 2016: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28052100/on-the-effect-of-planetary-stable-isotope-compositions-on-growth-and-survival-of-terrestrial-organisms
#16
Xueshu Xie, Roman A Zubarev
Isotopic compositions of reactants affect the rates of chemical and biochemical reactions. Usually it is assumed that heavy stable isotope enrichment leads to progressively slower reactions. Yet the effect of stable isotopes may be nonlinear, as exemplified by the "isotopic resonance" phenomenon. Since the isotopic compositions of other planets of Solar system, including Mars and Venus, are markedly different from terrestrial (e.g., deuterium content is ≈5 and ≈100 times higher, respectively), it is far from certain that terrestrial life will thrive in these isotopic conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28049191/adult-food-allergy-prevalence-reducing-questionnaire-bias
#17
Letícia A Silva, Anna Flávia M Silva, Âmela C Ribeiro, Augusto O Silva, Felipe A Vieira, Gesmar R S Segundo
BACKGROUND: Food allergy (FA) prevalence has increased in the last decades, but epidemiologic studies could show overestimated results. The objective of this study is to estimate the prevalence of immediate FA in adults in a region of Central Brazil, using a questionnaire to try to reduce misperceptions about FA reaction. METHODS: A cross-sectional study was conducted, enrolling an adult population aged 18-65 years comprised of families in a Central Brazilian city...
January 4, 2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/28046005/field-usable-lateral-flow-immunoassay-for-the-rapid-detection-of-white-spot-syndrome-virus-wssv
#18
Prabir Kumar Kulabhusan, Jyutika M Rajwade, Vimal Sugumar, Gani Taju, A S Sahul Hameed, Kishore M Paknikar
BACKGROUND: White spot disease (WSD), a major threat to sustainable aquaculture worldwide, is caused by White spot syndrome virus (WSSV). The diagnosis of WSD relies heavily on molecular detection of the virus by one-step PCR. These procedures are neither field-usable nor rapid enough considering the speed at which the virus spreads. Thus, development of a rapid, reliable and field-usable diagnostic method for the detection of WSSV infection is imperative to prevent huge economic losses...
2017: PloS One
https://www.readbyqxmd.com/read/28045016/the-three-herb-formula-shuang-huang-lian-stabilizes-mast-cells-through-activation-of-mitochondrial-calcium-uniporter
#19
Yuan Gao, Rui Hou, Qiaoling Fei, Lei Fang, Yixin Han, Runlan Cai, Cheng Peng, Yun Qi
Mast cells (MCs) are key effector cells of IgE-FcεRI- or MrgprX2-mediated signaling event. Shuang-Huang-Lian (SHL), a herbal formula from Chinese Pharmacopoeia, has been clinically used in type I hypersensitivity. Our previous study demonstrated that SHL exerted a non-negligible effect on MC stabilization. Herein, we sought to elucidate the molecular mechanisms of the prominent anti-allergic ability of SHL. MrgprX2- and IgE-FcεRI-mediated MC activation in vitro and in vivo models were developed by using compound 48/80 (C48/80) and shrimp tropomyosin (ST), respectively...
January 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28039304/allergen-valency-dose-and-fc%C3%AE%C2%B5ri-occupancy-set-thresholds-for-secretory-responses-to-pen-a-1-and-motivate-design-of-hypoallergens
#20
Avanika Mahajan, Lama A Youssef, Cédric Cleyrat, Rachel Grattan, Shayna R Lucero, Christopher P Mattison, M Frank Erasmus, Bruna Jacobson, Lydia Tapia, William S Hlavacek, Mark Schuyler, Bridget S Wilson
Ag-mediated crosslinking of IgE-FcεRI complexes activates mast cells and basophils, initiating the allergic response. Of 34 donors recruited having self-reported shrimp allergy, only 35% had significant levels of shrimp-specific IgE in serum and measurable basophil secretory responses to rPen a 1 (shrimp tropomyosin). We report that degranulation is linked to the number of FcεRI occupied with allergen-specific IgE, as well as the dose and valency of Pen a 1. Using clustered regularly interspaced palindromic repeat-based gene editing, human RBL(rαKO) cells were created that exclusively express the human FcεRIα subunit...
December 30, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
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