Elena Díaz-García, Kapil Nanwani-Nanwani, Sara García-Tovar, Enrique Alfaro, Eduardo López-Collazo, Manuel Quintana-Díaz, Francisco García-Rio, Carolina Cubillos-Zapata
Aneurysmal subarachnoid hemorrhage (aSAH) is an uncommon and severe subtype of stroke leading to the loss of many years of productive life. We analyzed NLRP3 activity as well as key components of the inflammasome cascade in monocytes and plasma from 28 patients with aSAH and 14 normal controls using flow cytometry, western blot, ELISA, and qPCR technologies. Our data reveal that monocytes from patients with aSAH present an overactivation of the NLRP3 inflammasome, which results in the presence of high plasma levels of interleukin (IL)-1β, IL-18, gasdermin D, and tissue factor...
July 11, 2022: Translational Stroke Research