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https://www.readbyqxmd.com/read/29684786/gram-marks-the-spot-for-stim-commentary-on-gram-domain-proteins-specialize-functionally-distinct-er-pm-contact-sites-in-human-cells
#1
Paula Nunes, Nicolas Demaurex
GRAM domain proteins were reported as novel ER-PM tethers defining specific membrane contact sites (MCS) subdomains. GRAMD2a pre-marks the sites occupied by STIM1 at MCS and its ablation impairs STIM1 translocation, but not store-operated Ca2+ entry. We discuss these apparently counterintuitive findings in the context of STIM/ORAI signaling at MCS.
April 18, 2018: Cell Calcium
https://www.readbyqxmd.com/read/29679726/late-presenting-dural-tear-incidence-risk-factors-and-associated-complications
#2
Wesley M Durand, J Mason DePasse, Eren O Kuris, JaeWon Yang, Alan H Daniels
BACKGROUND CONTEXT: Unrecognized and inadequately repaired intraoperative durotomies may lead to cerebrospinal fluid leak, pseudomeningocele, and other complications. Few studies have investigated durotomy that is unrecognized intraoperatively and requires additional postoperative management (hereafter, late-presenting dural tear [LPDT]), though estimates of LPDT range from 0.6 - 8.3 per 1,000 spinal surgeries. These single-center studies are based on relatively small sample sizes for an event of this rarity, all with <10 patients experiencing LPDT...
April 18, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29674140/impaired-ca-2-cycling-of-nonischemic-myocytes-contributes-to-sarcomere-dysfunction-early-after-myocardial-infarction
#3
Annette Kronenbitter, Florian Funk, Katarzyna Hackert, Simone Gorreßen, Dennis Glaser, Peter Boknik, Gereon Poschmann, Kai Stühler, Malgorzata Isić, Martina Krüger, Joachim P Schmitt
Changes in the nonischemic remote myocardium of the heart contribute to left ventricular dysfunction after ischemia and reperfusion (I/R). Understanding the underlying mechanisms early after I/R is crucial to improve the adaptation of the viable myocardium to increased mechanical demands. Here, we investigated the role of myocyte Ca2+ handling in the remote myocardium 24 h after 60 min LAD occlusion. Cardiomyocytes isolated from the basal noninfarct-related parts of wild type mouse hearts demonstrated depressed beat-to-beat Ca2+ handling...
April 16, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29669788/h19-induces-abdominal-aortic-aneurysm-development-and-progression
#4
Daniel Y Li, Albert Busch, Hong Jin, Ekaterina Chernogubova, Jaroslav Pelisek, Joakim Karlsson, Bengt Sennblad, Shengliang Liu, Shen Lao, Patrick Hofmann, Alexandra Bäcklund, Suzanne M Eken, Joy Roy, Per Eriksson, Brian Dacken, Deepak Ramanujam, Anne Dueck, Stefan Engelhardt, Reinier A Boon, Hans-Henning Eckstein, Joshua M Spin, Philip S Tsao, Lars Maegdefessel
Background -Long noncoding RNAs (lncRNAs) have emerged as critical molecular regulators in various biological processes and diseases. Here we sought to identify and functionally characterize lncRNAs as potential mediators in abdominal aortic aneurysm (AAA) development. Methods -We profiled RNA transcript expression in two murine AAA models, Angiotensin II (ANGII) infusion in ApoE-/- mice ( n =8) and porcine pancreatic elastase (PPE) instillation in C57BL/6 wildtype mice ( n =12). The lncRNA H19 was identified as one of the most highly up-regulated transcripts in both mouse aneurysm models compared to sham-operated controls...
April 18, 2018: Circulation
https://www.readbyqxmd.com/read/29624409/validation-of-a-host-response-assay-septicyte%C3%A2-lab-for-discriminating-sepsis-from-sirs-in-the-icu
#5
Russell R Miller Iii, Bert K Lopansri, John P Burke, Mitchell Levy, Steven Opal, Richard E Rothman, Franco R D'Alessio, Venkataramana K Sidhaye, Neil R Aggarwal, Robert Balk, Jared A Greenberg, Mark Yoder, Gourang Patel, Emily Gilbert, Majid Afshar, Jorge P Parada, Greg S Martin, Annette M Esper, Jordan A Kempker, Mangala Narasimhan, Adey Tsegaye, Stella Hahn, Paul Mayo, Tom van der Poll, Marcus J Schultz, Brendon P Scicluna, Peter Klein Klouwenberg, Antony Rapisarda, Therese A Seldon, Leo C McHugh, Thomas D Yager, Silvia Cermelli, Dayle Sampson, Victoria Rothwell, Richard Newman, Shruti Bhide, James T Kirk, Krupa Navalkar, Roy F Davis, Roslyn A Brandon, Richard B Brandon
RATIONALE: A molecular test to distinguish between sepsis and systemic inflammation of non-infectious etiology could potentially have clinical utility. OBJECTIVES: This study evaluated the diagnostic performance of a molecular host response assay (SeptiCyte™ LAB) designed to distinguish between sepsis and non-infectious systemic inflammation in critically ill adults. METHODS: The study employed a prospective, observational, non-interventional design, and recruited a heterogeneous cohort of adult critical care patients from seven sites in the USA (N=249)...
April 6, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29564112/catch-per-unit-effort-and-some-water-quality-parameters-of-lake-kalgwai-jigawa-state-nigeria
#6
Shola G Solomon, Victoria O Ayuba, Musa A Tahir, Victor T Okomoda
This study investigated the catch per unit effort and water quality of Lake Kalgwai Jigawa state, Nigeria for the period of 10 month (July 2012-June 2013). The man hours, gears used, and fish catches of the Lake was determined by assessing the fishermen operating on three major landing sites of the Lake, namely Marke (Site I), Dingare (Site II), and Kalgwai (Site III). Water samples from the landing sites were collected and analyzed. Concentration of fishers ranged from 41 (April) to 51 (September). The highest number of fishing hour was observed in August (64 hr), whereas the lowest was in March (49 hr)...
March 2018: Food Science & Nutrition
https://www.readbyqxmd.com/read/29561866/risk-prediction-for-staphylococcus-aureus-surgical-site-infection-following-cardiothoracic-surgery-a-secondary-analysis-of-the-v710-p003-trial
#7
Fleur P Paling, Karina Olsen, Kristin Ohneberg, Martin Wolkewitz, Vance G Fowler, Mark J DiNubile, Hasan S Jafri, Frangiscos Sifakis, Marc J M Bonten, Stephan J Harbarth, Jan A J W Kluytmans
BACKGROUND: Identifying patients undergoing cardiothoracic surgery at high risk of Staphylococcus aureus surgical site infection (SSI) is a prerequisite for implementing effective preventive interventions. The objective of this study was to develop a risk prediction model for S. aureus SSI or bacteremia after cardiothoracic surgery based on pre-operative variables. MATERIALS/METHODS: Data from the Merck Phase IIb/III S. aureus vaccine (V710-P003) clinical trial were analyzed...
2018: PloS One
https://www.readbyqxmd.com/read/29548786/implementation-of-an-abdominal-closure-bundle-to-reduce-surgical-site-infection-in-patients-on-a-gynecologic-oncology-service-undergoing-exploratory-laparotomy
#8
Shaina F Bruce, Danielle N Carr, Elizabeth R Burton, Joel I Sorosky, Mark S Shahin, Mary C Naglak, Mitchell I Edelson
OBJECTIVE: Surgical site infections (SSI) are associated with increased morbidity, mortality, and healthcare costs. This study investigated whether implementation of an abdominal closure bundle reduces surgical site infection rates. We aimed to identify sub-populations that would benefit the most from this intervention. METHODS: We conducted a retrospective cohort study of all patients that underwent exploratory laparotomy by a Gynecologic Oncologist from January 1, 2011 to April 1, 2017...
March 14, 2018: Gynecologic Oncology
https://www.readbyqxmd.com/read/29544511/online-advertising-and-marketing-claims-by-providers-of-proton-beam-therapy-are-they-guideline-based
#9
Mark T Corkum, Wei Liu, David A Palma, Glenn S Bauman, Robert E Dinniwell, Andrew Warner, Mark V Mishra, Alexander V Louie
BACKGROUND: Cancer patients frequently search the Internet for treatment options, and hospital websites are seen as reliable sources of knowledge. Guidelines support the use of proton radiotherapy in specific disease sites or on clinical trials. This study aims to evaluate direct-to-consumer advertising content and claims made by proton therapy centre (PTC) websites worldwide. METHODS: Operational PTC websites in English were identified through the Particle Therapy Co-Operative Group website...
March 15, 2018: Radiation Oncology
https://www.readbyqxmd.com/read/29534193/modified-ross-konno-procedure-in-children-subcoronary-implantation-technique-with-konno-incision-for-annular-and-subannular-hypoplasia
#10
Peter Murin, Nicodème Sinzobahamvya, Antonia Schulz, Victoria Lorenzen, Stanislav Ovroutski, Felix Berger, Joachim Photiadis, Mi-Young Cho
OBJECTIVES: The technique of subcoronary autograft implantation for the Ross procedure has shown excellent durability of aortic valve function in adults. However, its use in children with hypoplastic aortic annulus or multilevel left ventricular outflow tract obstruction (LVOTO) was traditionally precluded. We combined this technique with a Konno incision and evaluated LVOTO relief and durability of the autograft function in growing patients. METHODS: Between January 2012 and January 2017, 13 patients with severe LVOTO and hypoplastic aortic annulus underwent Ross-Konno procedure with subcoronary autograft implantation...
March 9, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29530071/a-multidisciplinary-team-oriented-intervention-to-increase-guideline-recommended-care-for-high-risk-prostate-cancer-a-stepped-wedge-cluster-randomised-implementation-trial
#11
Bernadette Brown, Jane Young, David P Smith, Andrew B Kneebone, Andrew J Brooks, Sam Egger, Miranda Xhilaga, Amanda Dominello, Dianne L O'Connell, Mary Haines
BACKGROUND: This study assessed whether a theoretically conceptualised tailored intervention centred on multidisciplinary teams (MDTs) increased clinician referral behaviours in line with clinical practice guideline recommendations. METHODS: Nine hospital Sites in New South Wales (NSW), Australia with a urological MDT and involvement in a state-wide urological clinical network participated in this pragmatic stepped wedge, cluster randomised implementation trial...
March 12, 2018: Implementation Science: IS
https://www.readbyqxmd.com/read/29519382/vascular-access-site-and-outcomes-in-58-870-patients-undergoing-percutaneous-coronary-intervention-with-a-previous-history-of-coronary-bypass-surgery-results-from-the-british-cardiovascular-interventions-society-national-database
#12
Tim Kinnaird, Richard Anderson, Sean Gallagher, James Cockburn, Alex Sirker, Peter Ludman, Mark de Belder, Samuel Copt, James Nolan, Azfar Zaman, Mamas Mamas
OBJECTIVES: Using the British Cardiovascular Intervention Society percutaneous coronary intervention (PCI) database, access site choice and outcomes of patients undergoing PCI with previous coronary artery bypass grafting (CABG) were studied. BACKGROUND: Given the influence of access site on outcomes, use of radial access in PCI-CABG warrants further investigation. METHODS: Data were analyzed from 58,870 PCI-CABG procedures performed between 2005 and 2014...
March 12, 2018: JACC. Cardiovascular Interventions
https://www.readbyqxmd.com/read/29513891/free-vascularized-fibula-grafting-in-the-operative-treatment-of-malignant-bone-tumors-of-the-upper-extremity-a-systematic-review-of-outcomes-and-complications
#13
Mark J Landau, Ido Badash, Christine Yin, Ram K Alluri, Ketan M Patel
BACKGROUND AND OBJECTIVES: Vascularized bone grafting after tumor resection can be an important component in the treatment of bony neoplasms of the upper extremity. The purpose of this study was to determine the outcomes of free vascularized fibula grafting (FVFG) in the treatment of upper extremity sarcomas. METHODS: A systematic review of the literature of FVFG used in the treatment of upper extremity sarcomas was performed. RESULTS: A total of 56 studies were included in final analysis...
March 7, 2018: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/29506815/evaluating-complication-rates-and-outcomes-among-infants-less-than-5kg-undergoing-traditional-percutaneous-endoscopic-gastrostomy-insertion-a-retrospective-chart-review
#14
Alisha R Fernandes, Tessa Elliott, Carter McInnis, Bethany Easterbrook, J Mark Walton
PURPOSE: Percutaneous endoscopic gastrostomy (PEG) enables enteral nutrition for patients with inadequate oral intake. Laparoscopic guidance of PEG insertion is used for high-risk populations, including in infants less than 5kg at insertion. This study aimed to assess complication rates with traditional PEG tube insertion in infants less than 5kg at a single tertiary care center. METHODS: A retrospective review of patients less than 5kg who underwent PEG insertion was conducted...
February 8, 2018: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29504975/ct-measurements-of-central-pulmonary-vasculature-as-predictors-of-severe-exacerbation-in-copd
#15
Ji Young Rho, David A Lynch, Young Ju Suh, Jeung Weon Nah, Jordan A Zach, Joyce D Schroeder, Christian W Cox, Russell P Bowler, Brett E Fenster, Mark T Dransfield, James M Wells, John E Hokanson, Douglas Curran-Everett, Andre Williams, MeiLan K Han, James D Crapo, Edwin K Silverman
To identify a predictive value for the exacerbation status of chronic obstructive pulmonary disease (COPD) subjects, we evaluated the relationship between pulmonary vascular measurements on chest CT and severe COPD exacerbation.Six hundred three subjects enrolled in the COPDGene population were included and divided into nonexacerbator (n = 313) and severe exacerbator (n = 290) groups, based on whether they had an emergency room visit and/or hospitalization for COPD exacerbation. We measured the diameter of the main pulmonary artery (MPA) and ascending aorta (AA) at 2 different sites of the MPA (the tubular midportion and bifurcation) on both axial images and multiplanar reconstructions...
January 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29481937/possible-mechanism-for-common-femoral-artery-occlusion-with-the-perclose-suture-device
#16
Mark Archie, Steven Farley
INTRODUCTION: Common femoral artery (CFA) occlusion by the Perclose suture device has been rarely reported in the literature. Authors and industry have no proposed mechanism. A review of patients who required operative repair may identify a possible mechanism for CFA occlusion. METHODS: At a single center, two patients were identified with CFA occlusion due to posterior CFA wall suturing. Vessel characteristics, angiographic, and intraoperative findings were reviewed...
February 23, 2018: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/29473313/vectorial-localization-of-peripheral-pulmonary-lesion-guided-by-electromagnetic-navigation-a-novel-method-for-diagnostic-surgical-resection-without-dye-marking
#17
Tong Qiu, Baohua Yu, Yunpeng Xuan, Haihong Luan, Wenjie Jiao
We describe a novel method using electromagnetic navigation bronchoscopy (ENB) without dye marking to perform vectorial localization for an impalpable peripheral lesion in the right lower lobe during diagnostic surgical resection in a 52-year-old woman. After the ENB registration process, the anesthetist changed the single-lumen endotracheal tube to a double-lumen endobronchial tube. Guided by the ENB system, the operator delivered the probe of the locatable guide to the planned site through the right lumen of the endobronchial tube...
February 23, 2018: Thoracic Cancer
https://www.readbyqxmd.com/read/29452835/a-comparison-of-preoperative-and-intraoperative-vein-mapping-sizes-for-arteriovenous-fistula-creation
#18
Samuel H Hui, Ryan Folsom, Lois A Killewich, Joel E Michalek, Mark G Davies, Lori L Pounds
BACKGROUND: Duplex ultrasound (DUS) mapping of the veins and arteries of the upper extremity is a well-established practice in arteriovenous fistula creation for long-term hemodialysis access. Previous publications have shown that vein diameters varying from 2 to 3 mm are predictive of success. Regional anesthesia is known to result in vasodilation and thus to increase the diameter of upper extremity veins. This study compares the sizes of veins measured by preoperative DUS mapping with those obtained after regional anesthesia to determine whether intraoperative DUS results in increased vein diameters and thus changes in the operative plan...
February 13, 2018: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29452281/incidence-and-risk-factors-of-post-operative-neurological-decline-after-complex-adult-spinal-deformity-surgery-results-of-the-scoli-risk-1-study
#19
Michael G Fehlings, So Kato, Lawrence G Lenke, Hiroaki Nakashima, Narihito Nagoshi, Christopher I Shaffrey, Kenneth M C Cheung, Leah Carreon, Mark B Dekutoski, Frank J Schwab, Oheneba Boachie-Adjei, Khaled M Kebaish, Christopher P Ames, Yong Qiu, Yukihiro Matsuyama, Benny T Dahl, Hossein Mehdian, Ferran Pellisé-Urquiza, Stephen J Lewis, Sigurd H Berven
BACKGROUND CONTEXT: Significant variability in neurologic outcomes following surgical correction for adult spinal deformity (ASD) has been reported. Risk factors for decline in neurologic motor outcomes are poorly understood. PURPOSE: The objective of the present investigation was to identify the risk factors for post-operative neurological motor decline in patients undergoing complex ASD surgery. STUDY DESIGN/SETTING: A prospective international multicenter cohort study...
February 13, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29428586/the-pedicled-internal-pudendal-artery-perforator-pipap-flap-for-ischial-pressure-sore-reconstruction-technique-and-long-term-outcome-of-a-cohort-study
#20
Catherine M Legemate, Monique van der Kwaak, David Gobets, Menno Huikeshoven, Paul P M van Zuijlen
BACKGROUND: The ischial region is the site most affected by pressure sores and has the highest recurrence and complication rates compared to other affected sites. We developed a practical and safe pedicled flap for reconstruction of ischial pressure sores based on the rich available perforators from the internal pudendal artery and the surplus of skin at the infragluteal fold. METHODS: A retrospective cohort study was conducted in all patients who underwent ischial pressure ulcer reconstruction using the PIPAP flap between March 2010 and March 2017...
February 7, 2018: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
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