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https://www.readbyqxmd.com/read/28533361/healthy-offspring-from-freeze-dried-mouse-spermatozoa-held-on-the-international-space-station-for-9-months
#1
Sayaka Wakayama, Yuko Kamada, Kaori Yamanaka, Takashi Kohda, Hiromi Suzuki, Toru Shimazu, Motoki N Tada, Ikuko Osada, Aiko Nagamatsu, Satoshi Kamimura, Hiroaki Nagatomo, Eiji Mizutani, Fumitoshi Ishino, Sachiko Yano, Teruhiko Wakayama
If humans ever start to live permanently in space, assisted reproductive technology using preserved spermatozoa will be important for producing offspring; however, radiation on the International Space Station (ISS) is more than 100 times stronger than that on Earth, and irradiation causes DNA damage in cells and gametes. Here we examined the effect of space radiation on freeze-dried mouse spermatozoa held on the ISS for 9 mo at -95 °C, with launch and recovery at room temperature. DNA damage to the spermatozoa and male pronuclei was slightly increased, but the fertilization and birth rates were similar to those of controls...
May 22, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28528381/metal-chelate-affinity-precipitation-with-thermo-responsive-polymer-for-purification-of-%C3%AE%C2%B5-poly-l-lysine
#2
Sipeng Li, Zhaoyang Ding, Jifu Liu, Xuejun Cao
ε-Poly-L-lysine (ε-PL) is a natural preservative for food processing industry. A thermo-responsive polymer, attached with Cu(2+) or Ni(2+), was prepared for metal-chelate affinity precipitation for purification of ε-PL. The low critical solution temperatures (LCSTs) of these polymers were close to the room temperature (31.0-35.0 °C). The optimal adsorption conditions were as follows: pH 4.0, 0 mol/L NaCl, ligand density 75.00 μmol/g, and 120 min. The ligand Cu(2+) showed a stronger affinity interaction with ε-PL and the highest adsorption amount reached 251...
May 20, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28527549/efficacy-of-intravenous-acetaminophen-in-periimplantation-pain-of-cardiac-electronic-devices-a-randomized-double-blinded-study
#3
Reza Mollazadeh, Mohammad Reza Eftekhari, Masoud Eslami
PURPOSE: Although intravenous acetaminophen has been administered to reduce postoperative pain, it has not been used during cardiac implantable electronic devices (CIEDs) implantation. DESIGN: This was a randomized double-blinded interventional study. METHODS: Thirty-two patients who were referred for new CIED implantation during July 2012 until April 2013 randomly received placebo or 1 g of intravenous acetaminophen. All patients were treated with local anesthesia...
June 2017: Journal of Perianesthesia Nursing: Official Journal of the American Society of PeriAnesthesia Nurses
https://www.readbyqxmd.com/read/28525790/quantitative-lc-hrms-determination-of-selected-cardiovascular-drugs-in-dried-blood-spots-as-an-indicator-of-adherence-to-medication
#4
Dennis Bernieh, Graham Lawson, Sangeeta Tanna
Dried blood spot (DBS) sampling was investigated as a means of obtaining micro-volume blood samples for the quantitative analyses of ten commonly UK prescribed cardiovascular drugs as an indicator of medication adherence. An 8mm disc was punched out from each DBS from calibration, quality control and volunteer samples and extracted using methanol containing the internal standard. Each extract was evaporated to dryness, the residue reconstituted in methanol:water (40:60v/v) containing 0.1% formic acid and analysed by LC-HRMS...
May 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28523364/-structured-patient-handovers-in-perioperative-medicine-rationale-and-implementation-in-clinical-practice
#5
M J Merkel, V von Dossow, B Zwißler
Clear and consistent communication is pivotal for well-functioning teamwork, in operating theatres as well as intensive care units. However, patient handovers significantly vary between specialties and locations. If communication is not well structured, it might increase the risk for mishaps and malpractice. Therefore, implementing structured handover protocols is pivotal. The perioperative setting is a high-risk environment that is prone to communication failures due to operational design (frequent change of shift due to working time restrictions) and a high work load and multitasking (operating room management, short surgery times, concurrent emergencies)...
May 18, 2017: Der Anaesthesist
https://www.readbyqxmd.com/read/28516365/clipping-of-ruptured-intracranial-aneurysms-in-a-hybrid-room-environment-a-case-control-study
#6
Philipp Dammann, Max Jägersberg, Zsolt Kulcsar, Ivan Radovanovic, Karl Schaller, Philippe Bijlenga
BACKGROUND: Hybrid room treatment (HRT) provides the surgeon immediate intraoperative angiography control of aneurysm occlusion and vessel patency. Since it is relatively resource demanding, in clinical routine HRT is reserved for elective cases. However, since its introduction in our department in 2008, several random cases of ruptured intracranial aneurysms (IAs) have been treated in the HR. This study aims to compare the clinical and radiological outcomes of these cases with cases treated conventionally using a matched pair analysis...
May 17, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28515519/desflurane-for-ambulatory-anaesthesia-a-comparison-with-sevoflurane-for-recovery-profile-and-airway-responses
#7
Kajal Sachin Dalal, Meghana Vijay Choudhary, Adit Jagdish Palsania, Pratibha Vinayak Toal
BACKGROUND AND AIMS: Desflurane and sevoflurane have low blood gas solubility co-efficients, allowing a rapid awakening from anaesthesia. However, desfluraneis pungent and may cause airway irritability. We compared desflurane and sevoflurane with respect to recovery and occurrence of adverse airway responses in spontaneously breathing patients while using the ProSeal™ laryngeal mask airway (LMA). METHODS: Ninety-four adult patients undergoing hysteroscopic procedures were divided into sevoflurane (S) group or desflurane (D) group...
April 2017: Indian Journal of Anaesthesia
https://www.readbyqxmd.com/read/28511313/-determination-of-thiocyanate-within-human-urine-by-ion-chromatography-method
#8
S Wang, X F Pan, W Zhao, H F Yan
Objective: To establish ion chromatography method to determine thiocyanate within urine of workers who were exposed to cyanide. Methods: After the workers work, used 50 ml centrifuge tube to collect the urine of workers about 20 ml.The urine were tested by centrifugation, dilution and filtration by C(18) column, thiocyanate was separated by AS(16) and mobile phase elution by KOH, detected by electrical circuitryconductivity detector, quantitative by the standard curve method. Results: The linear correlation coefficient of thiocyanate within the range of 0...
March 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/28505491/development-of-suitable-method-for-large-scale-urinary-glucocorticoid-analysis-by-liquid-chromatography-mass-spectrometry
#9
Akiko Matsumoto, Chisato Shimanoe, Keitaro Tanaka, Masayoshi Ichiba, Megumi Hara
Levels of urinary glucocorticoids and their concentration ratios have been analyzed as potential markers for various pathological statuses. Large-scale studies may possibly accelerate the investigations; however, a suitable method needs to be established. Analytical conditions for measurement of urinary glucocorticoids with LCMS were examined. Electrospray ionization in the positive ion mode was applied for detection of cortisol (precursor>product ion: 363.3>121.0), cortisol-d4 (internal standard, IS, 367...
July 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28504322/racial-differences-in-the-pain-management-of-children-recovering-from-anesthesia
#10
Olubukola O Nafiu, Wilson T Chimbira, Margaret Stewart, Kathleen Gibbons, L Kareen Porter, Paul I Reynolds
BACKGROUND: When pain management has been studied in settings such as pediatric emergency departments, racial disparities have been clearly identified. To our knowledge, this has not been studied in the pediatric perioperative setting. We sought to determine whether there are differences based on race in the administration of analgesia to children suffering from pain in the postanesthesia care unit. METHODS: This is a prospective, observational, study of 771 children aged 4-17 years who underwent elective outpatient surgery...
May 15, 2017: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/28504178/tetramethylguanidine-based-gels-and-colloids-of-cellulose
#11
Gonçalo V S M Carrera, Anabela Raymundo, Francisco M Braz Fernandes, Noémi Jordão, Isabel Sousa, Manuel Nunes da Ponte, Luís C Branco
Novel and stable gels of cellulose were produced. These gels are prepared at room temperature by combination of cellulose and tetramethylguanidine (TMG) in different ratios (1:1, 1:2, 1:3 in equivalents of alcohol groups of cellulose per number of molecules of TMG). Detailed NMR, SEM, rheological and XRD studies of these gels were carried out. The concentration of cellulose in the gel, temperature, frequency of oscillation and shear rate were used as variables in order to understand the fundamentals and optimize operational conditions, considering their possible use as matrices for CO2 capture...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28494910/the-effects-of-maternal-presence-during-anesthesia-induction-on-salivary-cortisol-levels-in-children-undergoing-tonsillectomy-and-or-adenoidectomy
#12
Hatice K Ozdogan, Sibel Cetinalp, Gokhan Kuran, Onder Tugal, Murat Tahiroglu, Ummuhan E Herdem, Suheyl Haytoglu
OBJECTIVE: Although parental presence during anesthesia induction is suggested to diminish operative stress level in children, there have been conflicting results about this strategy. The aim of this study was to evaluate the effects of maternal presence during induction on operative stress level in children who had tonsillectomy and/or adenoidectomy by determining the salivary cortisol levels. DESIGN: Non-randomized clinical study. SETTING: Preoperative and postoperative recovery rooms, operating room...
June 2017: Journal of Clinical Anesthesia
https://www.readbyqxmd.com/read/28494898/the-effect-of-desflurane-versus-propofol-anesthesia-on-postoperative-delirium-in-elderly-obese-patients-undergoing-total-knee-replacement-a-randomized-controlled-double-blinded-clinical-trial
#13
Pedro Tanaka, Stuart Goodman, Barbara R Sommer, William Maloney, James Huddleston, Hendrikus J Lemmens
STUDY OBJECTIVE: The goal of this study was to investigate the incidence of delirium, wake-up times and early post-operative cognitive decline in one hundred obese elderly patients undergoing total knee arthroplasty. DESIGN: Prospective randomized trial. SETTINGS: Operating room, postoperative recovery area, hospital wards. PATIENTS: 100 obese patients (ASA II and III) undergoing primary total knee replacement under general anesthesia with a femoral nerve block catheter...
June 2017: Journal of Clinical Anesthesia
https://www.readbyqxmd.com/read/28493430/first-case-of-bacteremia-caused-by-janibacter-hoylei
#14
Yong Kwan Lim, Oh Joo Kweon, Hye Ryoun Kim, Tae Hyoung Kim, Mi-Kyung Lee
Janibacter hoylei has previously been isolated only from an air sample in the upper atmosphere and clinical significance of J. hoylei was not yet established. Herein, we report a case of bacteremia caused by J. hoylei. An 8-week-old previously healthy male infant presented to the emergency room with fever. Blood culture yielded growth of Gram-positive bacilli and this microorganism could not be identified with conventional phenotypic methods. The isolate was identified by 16S rRNA gene sequencing, and the patient was successfully treated with vancomycin...
May 11, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28490046/rapid-homogeneous-immunoassay-to-quantify-gemcitabine-in-plasma-for-therapeutic-drug-monitoring
#15
Daniel Kozo, Matt W Ross, Justin Jarrah, Michael Barrett, Rebecca L Harney, Jodi B Courtney, Irina Baburina, Julianne L Holleran, Jan H Beumer, Godefridus J Peters, Richard J Honeywell, Salvatore J Salamone
BACKGROUND: Gemcitabine (2',2'-difluoro-2'-deoxycytidine) is a nucleoside analog used as a single agent and in combination regimens for the treatment of a variety of solid tumors. Several studies have shown a relationship between gemcitabine peak plasma concentration (Cmax) and hematological toxicity. An immunoassay for gemcitabine in plasma was developed and validated to facilitate therapeutic drug monitoring (TDM) by providing an economical, robust method for automated chemistry analyzers...
June 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28488591/red-emission-phosphor-s-brightness-deterioration-by-x-ray-and-brightness-recovery-phenomenon-by-heating
#16
Masaaki Nakamura, Koichi Chida, Yohei Inaba, Ryota Kobayashi, Masayuki Zuguchi
There are no feasible real-time and direct skin dosimeters for interventional radiology. One would be available if there were x-ray phosphors that had no brightness change caused by x-ray irradiation, but the emission of the Y2O3:Eu, (Y, Gd, Eu)BO3, and YVO4:Eu phosphors investigated in our previous study was reduced by x-ray irradiation. We found that the brightness of those phosphors recovered, and the purpose of this study is to investigate their recovery phenomena. It is expected that more kinds of phosphors could be used in x-ray dosimeters if the brightness changes caused by x-rays are elucidated and prevented...
May 10, 2017: Journal of Radiological Protection: Official Journal of the Society for Radiological Protection
https://www.readbyqxmd.com/read/28485954/camera-method-for-monitoring-a-mechanochromic-luminescent-%C3%AE-diketone-dye-with-rapid-recovery
#17
Tristan Butler, Alexander S Mathew, Michal Sabat, Cassandra L Fraser
Mechanochromic luminescent (ML) materials, which show a change in emission due to an applied mechanical stimulus, are useful components in a variety of applications, including organic light-emitting diodes, force sensors, optical memory storage, and next-generation lighting materials. While there are many different ML active derivatives, few show room temperature self-erasing. Thin films of the methoxy substituted β-diketone, gbmOMe, initially exhibited blue (428 nm) emission; however, green (478 nm) emission was observed after smearing...
May 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28479217/physiological-and-psychological-effects-of-testosterone-during-severe-energy-deficit-and-recovery-a-study-protocol-for-a-randomized-placebo-controlled-trial-for-optimizing-performance-for-soldiers-ops
#18
Stefan M Pasiakos, Claire E Berryman, J Philip Karl, Harris R Lieberman, Jeb S Orr, Lee M Margolis, John A Caldwell, Andrew J Young, Monty A Montano, William J Evans, Oshin Vartanian, Owen T Carmichael, Kishore M Gadde, Melissa Harris, Jennifer C Rood
BACKGROUND: The physiological consequences of severe energy deficit include hypogonadism and the loss of fat-free mass. Prolonged energy deficit also impacts physical performance, mood, attentiveness, and decision-making capabilities. This study will determine whether maintaining a eugonadal state during severe, sustained energy deficit attenuates physiological decrements and maintains mental performance. This study will also assess the effects of normalizing testosterone levels during severe energy deficit and recovery on gut health and appetite regulation...
May 4, 2017: Contemporary Clinical Trials
https://www.readbyqxmd.com/read/28476252/-zero-isolation-a-care-practice-oriented-towards-recovery
#19
Laurent Defromont, Coline Groulez, Guillaume François, Bruno Dekerf
Some institutions do not have an isolation room. Agitation is managed otherwise, with the idea that restraint or isolation can only be a terrible experience. The care pathway is therefore designed to favour autonomy and to limit restrictions of freedom. In this context, professionals are in constant contact with the user. Example of a unit which advocates this concept of rehabilitation.
May 2017: Soins. Psychiatrie
https://www.readbyqxmd.com/read/28475760/evaluating-patients-walking-capacity-during-hospitalization-for-lung-cancer-resection%C3%A2
#20
Pedro A Esteban, Nieves Hernández, Nuria M Novoa, Gonzalo Varela
OBJECTIVES: The goal of this study was to describe non-supervised daily physical activity in patients during the period immediately following anatomical lung resection. METHODS: The study was an observational study on 50 consecutive patients (33 men) admitted for anatomical lung resection over a 4-month period. All cases were approached using a minimally invasive technique. Patients were instructed by nursing and physiotherapy staff and asked to wear a portable pedometer (Omron HJ-720 T-E2) from admission until hospital discharge, excluding the day of the operation and the first hours in the recovery room...
May 4, 2017: Interactive Cardiovascular and Thoracic Surgery
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