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https://www.readbyqxmd.com/read/29341505/stability-integrity-and-recovery-rate-of-cellular-nucleic-acids-preserved-in-a-new-liquid-based-cytology-medium
#1
Guodong Zhao, Zhaocheng Liu, Yaping Tian, Minsheng Zhu, Shouli Wang, Hui Wang, Shen Gao, Yong Ma, Minxue Zheng
BACKGROUND: Liquid-based cytology (LBC) has replaced the conventional Papanicolaou test in cervical cancer screening. The cervical swab specimens collected in LBC media can also be used for additional analyses including high-risk HPV (HR-HPV) test, DNA methylation analysis, and HPV E6/E7 mRNA test. METHODS: The stability, integrity, and recovery rate of cellular DNA and RNA after storage at different conditions were evaluated by a quantitative real-time PCR (qPCR) based HR-HPV test, reverse transcription qPCR (RT-qPCR), and agarose gel electrophoresis...
January 16, 2018: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29340129/protective-isolation-for-patients-with-haematological-malignancies-a-pilot-study-investigating-patients-distress-and-use-of-time
#2
O Annibali, C Pensieri, V Tomarchio, V Biagioli, M Pennacchini, A Tendas, V Tambone, M C Tirindelli
Background: Patients with haematological malignancies are often hospitalized in protective isolation until full neutrophil recovery in order to prevent infections. This descriptive pilot study evaluate the level of isolation-related distress and the use of free time in a sample of Italian onco-haematological patients who were hospitalized in protective isolation. Materials and Methods: Participants were 18 patients hospitalized in hematologic ward to receive induction therapy (n=12) or autologous stem cell transplant (n=6)...
October 1, 2017: International Journal of Hematology-oncology and Stem Cell Research
https://www.readbyqxmd.com/read/29335151/-tools-to-overcome-anxiety-in-paediatric-surgery
#3
Nolwenn Febvre
An association of caregivers carried out several actions to combat children's anxiety in paediatric surgery, including anaesthetic masks to cover in stickers, a tactile application on the operating pathway and a cuddly toy in the recovery room. These tools help children at the moment of being separated from their parents and all along their care pathway. They also encourage caregivers to adjust their practices to improve the care provided to the families.
January 2018: Soins. Pédiatrie, Puériculture
https://www.readbyqxmd.com/read/29333775/-effect-of-dexmedetomidine-on-emergence-agitation-after-general-anesthesia-in-children-undergoing-odontotherapy-in-day-surgery-operating-room
#4
Luo Lin, Zhang Yueming, Li Meisheng, Wang Jiexue, Ji Yang
OBJECTIVE: To study the effectiveness of dexmedetomidine used for general anesthesia maintenance in children undergoing odontotherapy in day-surgery operating room in reducing the incidence of emergence agitation (EA). METHODS: Eighty children undergoing odontotherapy and under general anesthesia in day-surgery operating room were randomized into two groups, group A (n=40) and group B (n=40). Each patient in group A was administered with a bolus dose of dexmedetomidine (1...
December 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29332428/the-effectiveness-of-preoperative-preparation-for-improving-perioperative-outcomes-in-children-and-caregivers
#5
Chia-Jung Lin, Hsin-Ping Liu, Pei-Ya Wang, Mei-Hua Yu, Mei-Chun Lu, Ling-Yu Hsieh, Tzu-Chia Lin
Most children experience significant anxiety during the preoperative period. Greater preoperative anxiety may be related to a higher incidence of negative behaviors. This study aimed to develop a family-centered preoperative preparation program and to evaluate the effects of this program on children's preoperative emotional behaviors, postoperative behavior, and posthospital behavior, and on caregiver anxiety. A prospective, randomized controlled study was conducted. The population consisted of children who underwent minor surgery and their caregivers...
January 1, 2018: Behavior Modification
https://www.readbyqxmd.com/read/29328748/analysis-of-neuro-theatre-utilisation-and-reasons-for-cancellation-to-improve-efficiency-and-productivity
#6
Feras Sharouf, Asfand Baig Mirza, Vazira Moosajee, Sarah Plummer, Imran M Bhatti, Malik Zaben
In neurosurgery, much emphasis has recently been placed on theatre cancellation and time utilization as a key hospital management performance indicator. We sought to evaluate our unit's theatre throughput efficiency and identify the causes of elective surgery cancellations. We retrospectively audited all scheduled elective neurosurgical procedures over a period of nine months. Mean theatre utilization time was 47.0%. The common causes of cancellations were lack of theatre time (32%), non-availability of beds in recovery room (18...
April 2017: Journal of Perioperative Practice
https://www.readbyqxmd.com/read/29325515/safer-chairs-for-elderly-patients-design-evaluation-using-electromyography-and-force-measurement
#7
Shabboo Valipoor, Debajyoti Pati, Matt S Stock, Doug Bazuin
A vast majority of patient fall events in hospitals involve the elderly. In inpatient care settings, despite the risk of fall, patients are encouraged to leave their bed, move around their room, and sit on their chair to progress in their healing. Despite the vital role of patient chair design in improving recovery, few studies have examined the ergonomic requirements of safe patient chairs. This study examined the impact of manipulating horizontal and vertical positions of armrests in a test chair on required physical effort during Stand-to-Sit-to-Stand (St-Si-St) transitions among fifteen elderly women...
January 11, 2018: Ergonomics
https://www.readbyqxmd.com/read/29323632/recycling-of-neodymium-enhanced-by-functionalized-magnetic-ferrite
#8
Yao-Jen Tu, Chen-Feng You, Sheng-Chung Lo, Ting-Shan Chan, Chuan-Hsiung Chung
This study systematically evaluates Neodymium (Nd) recovery from actual seawaters and wastewater using functionalized magnetic ferrite (3-mercaptopropionic acid-tetraethyl orthosilicate ferrite, MPA-TEOS-ferrite). The recovery of Nd by MPA-TEOS-ferrite displayed an L-shaped nonlinear isotherm, suggesting limiting binding sites on the adsorbent surface. At room temperature, a significantly recovery of Nd by MPA-TEOS-ferrite increased from 8.99 to 99.99% with increasing pH (2.89 to 8.16) and an enhanced maxima Nd recovery capacity was observed on MPA-TEOS-ferrite (25...
January 11, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29318171/mobile-web-based-follow-up-for-postoperative-acl-reconstruction-a-single-center-experience
#9
James Higgins, John Semple, Lucas Murnaghan, Sarah Sharpe, John Theodoropoulos
Background: The initial 6 weeks after surgery has been identified as an area for improvement in patient care. During this period, the persistence of symptoms that go unchecked can lead to unscheduled emergency room and clinic visits, calls to surgeons' offices, and readmissions. Purpose: To analyze postoperative data from a previous study examining postoperative outcomes in 2 patient populations following breast reconstruction and anterior cruciate ligament (ACL) reconstruction with use of a patient-centered mobile application...
December 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29316240/determination-of-3-chloro-1-2-propanediol-in-polyamideamine-epichlorohydrin-resin-solution-by-reaction-based-headspace-gas-chromatography
#10
Ning Yan, Xiao-Fang Wan, Xin-Sheng Chai, Run-Quan Chen
We report on a headspace gas chromatographic method for determining the content of 3-chloro-1,2-propanediol in polyamideamine epichlorohydrin resin solution. It was based on quantitatively converting 3-chloro-1,2-propanediol to formaldehyde by periodate oxidation in a closed headspace sample vial at a room temperature for 10 min, and then to methanol by borohydride reduction at 90°C for 40 min followed by the headspace gas chromatographic measurement. The results showed that the present method has an excellent measurement precision (relative standard deviation < 2...
January 8, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29310829/-sugammadex-by-ideal-body-weight-versus-20-and-40-corrected-weight-in-bariatric-surgery-double-blind-randomized-clinical-trial
#11
Nádia Maria da Conceição Duarte, Ana Maria Menezes Caetano, Silvio da Silva Caldas Neto, Getúlio Rodrigues de Oliveira Filho, Gustavo de Oliveira Arouca, Josemberg Marins Campos
BACKGROUND AND OBJECTIVES: The weight parameters for use of sugammadex in morbidly obese patients still need to be defined. METHODS: A prospective clinical trial was conducted with sixty participants with body mass index≥40kg·m-2 during bariatric surgery, randomized into three groups: ideal weight (IW), 20% corrected body weight (CW20) and 40% corrected body weight (CW40). All patients received total intravenous anesthesia. Rocuronium was administered at dose of 0...
January 5, 2018: Revista Brasileira de Anestesiologia
https://www.readbyqxmd.com/read/29309941/barriers-to-enhanced-recovery-after-surgery-after-laparoscopic-sleeve-gastrectomy
#12
Arinbjorn Jonsson, Edward Lin, Lava Patel, Ankit D Patel, Jamil L Stetler, Heather Prayor-Patterson, Arvinpal Singh, Jahnavi K Srinivasan, John F Sweeney, S Scott Davis
BACKGROUND: Enhanced recovery (ERAS) protocols lead to expedited discharges and decreased cost. Bariatric centers have adopted such programs for safely discharging patients after sleeve gastrectomy (LSG) on the first postoperative day (POD1). Despite pathways, some bariatric patients cannot be discharged on POD1. METHODS: Retrospective review of patients undergoing LSG, between 2013 through 2016, in a center of excellence utilizing a standardized enhanced recovery pathway...
January 5, 2018: Journal of the American College of Surgeons
https://www.readbyqxmd.com/read/29306190/anchoring-ultrafine-pd-nanoparticles-and-sno2-nanoparticles-on-reduced-graphene-oxide-for-high-performance-room-temperature-no2-sensing
#13
Ziying Wang, Tong Zhang, Chen Zhao, Tianyi Han, Teng Fei, Sen Liu, Geyu Lu
In this paper, we demonstrate room-temperature NO2 gas sensors using Pd nanoparticles (NPs) and SnO2 NPs decorated reduced graphene oxide (Pd-SnO2-RGO) hybrids as sensing materials. It is found that ultrafine Pd NPs and SnO2 NPs with particle sizes of 3-5 nm are attached to RGO nanosheets. Compared to SnO2-RGO hybrids, the sensor based on Pd-SnO2-RGO hybrids exhibited higher sensitivity at room temperature, where the response to 1 ppm NO2 was 3.92 with the response time and recovery time being 13 s and 105 s...
December 28, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29303456/bobble-head-doll-syndrome-report-of-2-cases-and-a-review-of-the-literature-with-video-documentation-of-the-clinical-phenomenon
#14
Bryan Renne, Stefan Rueckriegel, Sudheesh Ramachandran, Julia Radic, Paul Steinbok, Ash Singhal
Bobble-head doll syndrome (BHDS) is a rare pediatric movement disorder presenting with involuntary 2- to 3-Hz head movements. Common signs and symptoms also found on presentation include macrocephaly, ataxia, developmental delay, optic disc pallor or atrophy, hyperreflexia, tremor, obesity, endocrinopathy, visual disturbance or impairment, headache, and vomiting, among others. The syndrome is associated with suprasellar cysts, third ventricular cysts, or aqueductal obstruction, along with a few other less common conditions...
January 5, 2018: Journal of Neurosurgery. Pediatrics
https://www.readbyqxmd.com/read/29299540/development-of-a-fluorescent-capillary-biosensor-based-on-self-assembled-aunp-ldh-for-micro-volume-intracellular-pyruvate
#15
Ya-Xiong Luo, Qiao-Jing Li, Lang Zhou, Yong-Sheng Li, Xiu-Feng Gao
Herein, a fluorescent capillary biosensor was developed for quantifying micro-volume intracellular pyruvate (PA), in which AuNPs and lactate dehydrogenase (LDH) were modified on the inner surface of an amination capillary (20 μL) via a self-assembly technique. The PA concentration was quantified by the change in the value of the fluorescence of NADH after sucking a mixed solution of the sample and NADH into the biosensor. This study investigated factors including the degree of protonation of the amino groups on the surface of the capillary, the AuNP concentration and time for self-assembly, the activity concentration and time for the LDH self-assembly, the flow rate and acidity for LDH immobilization, pH, temperature, and reaction time for the NADH/PA/LDH reaction system...
January 4, 2018: Analyst
https://www.readbyqxmd.com/read/29295557/amine-functionalized-sugarcane-bagasse-a-renewable-catalyst-for-efficient-continuous-flow-knoevenagel-condensation-reaction-at-room-temperature
#16
Yanhui Qiao, Junjiang Teng, Shuangfei Wang, Hao Ma
A biomass-based catalyst with amine groups (-NH₂), viz., amine-functionalized sugarcane bagasse (SCB-NH₂), was prepared through the amination of sugarcane bagasse (SCB) in a two-step process. The physicochemical properties of the catalyst were characterized through FT-IR, elemental analysis, XRD, TG, and SEM-EDX techniques, which confirmed the -NH₂ group was grafted onto SCB successfully. The catalytic performance of SCB-NH₂ in Knoevenagel condensation reaction was tested in the batch and continuous flow reactions...
December 24, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29287970/closed-system-manufacturing-of-cd19-and-dual-targeted-cd20-19-chimeric-antigen-receptor-t-cells-using-the-clinimacs-prodigy-device-at-an-academic-medical-center
#17
Fenlu Zhu, Nirav Shah, Huiqing Xu, Dina Schneider, Rimas Orentas, Boro Dropulic, Parameswaran Hari, Carolyn A Keever-Taylor
BACKGROUND AIMS: Multiple steps are required to produce chimeric antigen receptor (CAR)-T cells, involving subset enrichment or depletion, activation, gene transduction and expansion. Open processing steps that increase risk of contamination and production failure are required. This complex process requires skilled personnel and costly clean-room facilities and infrastructure. Simplified, reproducible CAR-T-cell manufacturing with reduced labor intensity within a closed-system is highly desirable for increased availability for patients...
December 26, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29287876/effect-of-preoperative-visiting-operation-room-on-emergence-agitation-in-preschool-children-under-sevoflurane-anesthesia
#18
Qiaosheng Zhong, Xianfeng Qu, Chuanhua Xu
BACKGROUND: Emergence agitation (EA) is a common complication in children during recovery from sevoflurane anesthesia with an high incidence. The main objective of this study was to compare the effects of preoperative visiting operation room (PVOR) to administration of propofol at the end of anesthesia on EA in preschool children under sevoflurane anesthesia. METHODS: Sixty-nine preschool children aged from 3 to 6 years scheduled for tonsillectomy under sevoflurane anesthesia were randomly allocated to one of the three groups to receive either PVOR (Group PV), routine preoperative visit (Group RV) or routine preoperative visit plus propofol (Group RP), 23 patients were included in each group...
January 2018: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/29286452/esophageal-heat-transfer-for-patient-temperature-control-and-targeted-temperature-management
#19
Melissa I Naiman, Maria Gray, Joseph Haymore, Ahmed F Hegazy, Andrej Markota, Neeraj Badjatia, Erik B Kulstad
Controlling patient temperature is important for a wide variety of clinical conditions. Cooling to normal or below normal body temperature is often performed for neuroprotection after ischemic insult (e.g. hemorrhagic stroke, subarachnoid hemorrhage, cardiac arrest, or other hypoxic injury). Cooling from febrile states treats fever and reduces the negative effects of hyperthermia on injured neurons. Patients are warmed in the operating room to prevent inadvertent perioperative hypothermia, which is known to cause increased blood loss, wound infections, and myocardial injury, while also prolonging recovery time...
November 21, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29284864/effect-of-intravenous-magnesium-sulfate-on-the-minimum-alveolar-concentrations-of-desflurane-using-bispectral-index-monitoring-a-prospective-randomized-double-blind-controlled-study
#20
Mohd Rameez Riaz, Vikram Mahajan, Sadaf Syed, Riyaz Ahmad
Background: Magnesium sulfate has been implicated to influence the minimum alveolar concentration (MAC) of various volatile anesthetics, but its effect on desflurane remains unanswered so far. Aim: To study the effect of perioperative intravenous magnesium sulfate on MAC of desflurane using bispectral index (BIS) monitoring. Settings: Operating room of a tertiary care hospital. Design: A prospective, randomized, controlled, double-blind clinical trial...
October 2017: Anesthesia, Essays and Researches
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