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https://www.readbyqxmd.com/read/23469028/identification-of-fn1bp1-as-a-novel-cell-cycle-regulator-through-modulating-g1-checkpoint-in-human-hepatocarcinoma-hep3b-cells
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Mei Liu, Ronghua Wu, Fuye Yang, Tao Wang, Pingping Zhang, Jianren Gu, Dafang Wan, Shengli Yang
A novel human gene, FN1BP1 (fibronectin 1 binding protein 1), was identified using the human placenta cDNA library. Northern blotting showed a transcript of ∼2.8 kb in human placenta, liver, and skeletal muscle tissues. This mRNA transcript length was similar to the full FN1BP1 sequence obtained previously. We established a conditionally induced stable cell line of Hep3B-Tet-on-FN1BP1 to investigate the preliminary function and mechanism of the secretory FN1BP1 protein. Cell-proliferation and colony-conformation assays demonstrated that FN1BP1 protein suppressed Hep3B cell growth and colonization in vitro...
2013: PloS One
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