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https://www.readbyqxmd.com/read/29224159/quantitation-of-dna-methylation-by-quantitative-multiplex-methylation-specific-pcr-qm-msp-assay
#1
Mary Jo Fackler, Saraswati Sukumar
The defining feature of the Quantitative Multiplex Methylation-Specific PCR (QM-MSP) method to sensitively quantify DNA methylation is the two-step PCR approach for a multiplexed analysis of a panel of up to 12 genes in clinical samples with minimal quantities of DNA. In the first step, for up to 12 genes tested, one pair of gene-specific primers (forward and reverse) amplifies the methylated and unmethylated copies of the same gene simultaneously and in multiplex, in one PCR reaction. This methylation-independent amplification step produces amplicons of up to 109 copies per μL after 36 cycles of PCR...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29223959/performance-and-operational-characteristics-of-point-of-care-tests-for-the-diagnosis-of-urogenital-gonococcal-infections
#2
REVIEW
Rebecca J Guy, Louise M Causer, Jeffrey D Klausner, Magnus Unemo, Igor Toskin, Anna M Azzini, Rosanna W Peeling
BACKGROUND: In 2012, there was an estimated 78 million new cases of gonorrhoea globally. Untreated infection may lead to reproductive and neonatal morbidity and facilitate HIV transmission. Diagnosis and treatment are a priority for control and prevention, yet use of point-of-care tests (POCTs) for Neisseria gonorrhoeae (NG) is limited. OBJECTIVES: To review the performance and operational characteristics of NG POCTs for diagnosis of urogenital gonorrhoea. METHODS: We compiled and synthesised findings from two separate systematic reviews which included evaluations published until August 2015...
December 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29223715/development-of-two-fluorine-18-labeled-pet-radioligands-targeting-pde10a-and-in-vivo-pet-evaluation-in-nonhuman-primates
#3
Vladimir Stepanov, Akihiro Takano, Ryuji Nakao, Nahid Amini, Shotaro Miura, Tomoaki Hasui, Haruhide Kimura, Takahiko Taniguchi, Christer Halldin
INTRODUCTION: Phosphodiesterase 10A (PDE10A) is a member of the PDE enzyme family that degrades cyclic adenosine and guanosine monophosphates (cAMP and cGMP). Based on the successful development of [11C]T-773 as PDE10A positron emission tomography (PET) radioligand, in this study our aim was to develop and evaluate fluorine-18 analogs of [11C]T-773. METHODS: [18F]FM-T-773-d2 and [18F]FE-T-773-d4 were synthesized from the same precursor used for 11C-labeling of T-773 in a two-step approach via 18F-fluoromethylation and 18F-fluoroethylation, respectively, using corresponding deuterated synthons...
October 23, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/29222172/proximal-aberrant-crypt-foci-associate-with-synchronous-neoplasia-and-are-primed-for-neoplastic-progression
#4
David A Drew, Allen Mo, James J Grady, Richard G Stevens, Joel B Levine, Bruce M Brenner, Joseph C Anderson, Faripour Forouhar, Michael J O'Brien, Thomas J Devers, Daniel W Rosenberg
Aberrant crypt foci (ACF) are the earliest morphologically identifiable lesion found within the human colon. Despite their relatively high frequency in the distal colon, few studies have examined the molecular characteristics of ACF within the proximal colon. In the following study, clinical participants (n=184) were screened for ACF using high-definition chromoendoscopy with contrast dye-spray. Following pathological confirmation, ACF biopsies were subjected to laser-capture microdissection (LCM) and epithelial cells were evaluated for somatic mutations with a customized colorectal cancer mutation panel using DNA-mass spectrometry...
December 8, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29221645/primary-care-led-weight-management-for-remission-of-type-2-diabetes-direct-an-open-label-cluster-randomised-trial
#5
Michael Ej Lean, Wilma S Leslie, Alison C Barnes, Naomi Brosnahan, George Thom, Louise McCombie, Carl Peters, Sviatlana Zhyzhneuskaya, Ahmad Al-Mrabeh, Kieren G Hollingsworth, Angela M Rodrigues, Lucia Rehackova, Ashley J Adamson, Falko F Sniehotta, John C Mathers, Hazel M Ross, Yvonne McIlvenna, Renae Stefanetti, Michael Trenell, Paul Welsh, Sharon Kean, Ian Ford, Alex McConnachie, Naveed Sattar, Roy Taylor
BACKGROUND: Type 2 diabetes is a chronic disorder that requires lifelong treatment. We aimed to assess whether intensive weight management within routine primary care would achieve remission of type 2 diabetes. METHODS: We did this open-label, cluster-randomised trial (DiRECT) at 49 primary care practices in Scotland and the Tyneside region of England. Practices were randomly assigned (1:1), via a computer-generated list, to provide either a weight management programme (intervention) or best-practice care by guidelines (control), with stratification for study site (Tyneside or Scotland) and practice list size (>5700 or ≤5700)...
December 4, 2017: Lancet
https://www.readbyqxmd.com/read/29220051/improving-extraction-and-post-purification-concentration-of-membrane-proteins
#6
Hasin Feroz, HyeYoung Kwon, Jing Peng, Hyeonji Oh, Bryan Ferlez, Carol S Baker, John H Golbeck, Guillermo C Bazan, Andrew L Zydney, Manish Kumar
Membrane proteins (MPs), despite being critically important drug targets for the pharmaceutical industry, are difficult to study due to challenges in obtaining high yields of functional protein. Most current extraction efforts use specialized non-ionic detergents to solubilize and stabilize MPs, with MPs being concentrated by ultrafiltration (UF). However, many detergents are retained during the UF step, which can destabilize MPs and/or interfere with their characterization. Here, we studied the influence of detergent selection on the extraction and UF-based concentration of biomedically-relevant MPs, the light-driven sodium and chloride transporters, KR2 and halorhodopsin (pHR) which are also model proteins for more complex mammalian rhodopsins...
December 8, 2017: Analyst
https://www.readbyqxmd.com/read/29219747/using-magnetic-materials-to-harvest-microalgal-biomass-evaluation-of-harvesting-and-detachment-efficiency
#7
L-D Zhu, Erkki Hiltunen, Zhaohua Li
Using naked iron oxide (Fe3O4) and yttrium iron oxide (Y3Fe5O12) nanoparticles as flocculants, the harvesting efficiency of Chlorella vulgaris biomass was investigated. The harvesting process includes two steps, which are the separation of microalgae from the culture solution with the magnetic nanoparticles and then the separation of the algae from the magnetic nanoparticles. The optimal dosages and pH values for the magnetic harvesting of microalgal biomass were determined. Results showed that Y3Fe5O12 nanoparticles were more efficient in microalgal biomass harvesting than Fe3O4 nanoparticles...
December 8, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29218684/feasibility-of-arthroscopic-autologous-chondrocyte-implantation-in-the-hip-using-an-injectable-hydrogel
#8
Steffen Thier, Florian Baumann, Christel Weiss, Stefan Fickert
INTRODUCTION: In the long term the treatment of articular cartilage defects of the hip has the most direct impact on the postoperative outcome and should diminish degenerative changes caused by different pathologies. The purpose of this prospective feasibility study is to describe technical aspects of arthroscopic, injectable autologous chondrocyte implantation in the hip and to report the short-term outcome. METHODS: Full-thickness cartilage defects of 13 patients were treated arthroscopically with an injectable autologous chondrocyte transplantation product (Novocart Inject, Tetec) in a 2-step surgical procedure...
December 8, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/29217738/radiolabeled-4-fluoro-3-iodobenzyl-guanidine-improves-imaging-and-targeted-radionuclide-therapy-of-norepinephrine-transporter-expressing-tumors
#9
Aiko Yamaguchi, Hirofumi Hanaoka, Tetsuya Higuchi, Yoshito Tsushima
Fluorine-18 (18F)/Iodine-131 (131I)-labeled (4-Fluoro-3-iodobenzyl)guanidine (FIBG) have been a promising yet unattainable derivative of radioiodine-labeled meta-iodobenzylguanidine (MIBG), because of the complex radiofluorination method. Here, we proposed a 2-step radiosynthetic method for 18F-FIBG, and evaluated the diagnostic and therapeutic potential of 18F-FIBG and 131I-FIBG in a pheochromocytoma model (PC-12). Methods:18F-FIBG was prepared from a (mesityl)(aryl)iodonium salt precursor in the presence of a copper-catalyst...
December 7, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29216349/developing-a-standard-set-of-patient-centred-outcomes-for-inflammatory-bowel-disease-an-international-cross-disciplinary-consensus
#10
Andrew H Kim, Charlotte Roberts, Brian G Feagan, Rupa Banerjee, Willem Bemelman, Keith Bodger, Marc Derieppe, Axel Dignass, Richard Driscoll, Ray Fitzpatrick, Janette Gaarentstroom-Lunt, Peter D Higgins, Paulo Gustavo Kotze, Jillian Meissner, Marian O'Connor, Zhi-Hua Ran, Corey A Siegel, Helen Terry, Welmoed K van Deen, C Janneke van der Woude, Alandra Weaver, Suk-Kyun Yang, Bruce E Sands, Séverine Vermeire, Simon Pl Travis
Background and Aims: Success in delivering value-based healthcare involves measuring outcomes that matter most to patients. Our aim was to develop a minimum Standard Set of patient-centred outcome measures for inflammatory bowel disease (IBD), for use in different healthcare settings. Methods: An international working group (n=25) representing patients, patient associations, gastroenterologists, surgeons, specialist nurses, IBD registries and patient-reported outcome measure (PROM) methodologists participated in a series of teleconferences incorporating a modified Delphi process...
December 5, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/29216237/the-nutraceutical-benefits-of-subfractions-of-abelmoschus-esculentus-in-treating-type-2-diabetes-mellitus
#11
Chien-Ning Huang, Chau-Jong Wang, Chih-Li Lin, Hui-Ting Lin, Chiung-Huei Peng
Abelmoschus esculentus (AE), a commonly consumed vegetable, is well-known for its anti-hyperglycemic effects. However, few scientific reports have identified its targets because mucilage increases the difficulty of manipulation. We recently reported extraction steps to obtain subfractions of AE, which were found to attenuate the adverse effects of high glucose and fatty acid in vitro. In this study, we used modified extraction steps and type 2 diabetic rats to explore whether AE subfractions can improve the metabolic disturbances caused by insulin resistance in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/29215966/effect-of-koh-activation-in-the-desulfurization-process-of-activated-carbon-prepared-by-sewage-sludge-corn-straw
#12
Fan Zeng, Xiaofeng Liao, Hui Hu, Li Liao
Series sludge-straw based activated carbons were prepared by sewage sludge and corn straw with potassium hydroxide activation, and the desulfurization performance of activated carbons were studied. To obtain the best desulfurization performance, the optimum ratio between the raw materials and the activator was investigated. The results showed that as the mass ratio of sewage sludge, corn straw and potassium hydroxide was 3:7:2, the activated carbon obtained the best breakthrough and saturation sulfur sorption capacity, which were 12...
December 7, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/29215516/clomiphene-stair-step-protocol-for-women-with-polycystic-ovary-syndrome
#13
Tiffanny Jones, Jacqueline R Ho, Marc Gualtieri, Janet Bruno-Gaston, Karine Chung, Richard J Paulson, Kristin A Bendikson
OBJECTIVE: To compare time to ovulation, ovulation rates, and side effect profile of traditional and the stair-step protocol for ovulation induction using clomiphene citrate in women with polycystic ovary syndrome (PCOS). METHODS: We performed a retrospective study of women seeking care for infertility with a diagnosis of PCOS at a university-based infertility clinic from July 2012 to July 2014. We included patients who were resistant to the initial starting dose of 50 mg clomiphene...
December 4, 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29214619/real-time-interrater-reliability-of-a-novel-musculoskeletal-readiness-screening-tool
#14
Mark D Thelen, Shane L Koppenhaver, Shanee E Allen, Michael U Bolduc, Riley K Quan, Anne E Sidwell
Military service members receive regular screenings for a variety of health conditions, but a field-expedient and military-specific screening tool that identifies an individual's risk for injury has not yet been identified. The purpose of this study is to describe the conduct of a novel musculoskeletal readiness screening tool (MRST) and evaluate the real-time interrater reliability of the MRST when scored by raters with differing levels of medical experience. MATERIALS/METHODS: This study included a convenience sample of 40 active duty military participants (30 male, 10 female, mean age 29...
October 2017: U.S. Army Medical Department Journal
https://www.readbyqxmd.com/read/29214250/observations-of-a-streak-texture-in-the-hybrid-aligned-smectic-c-phase
#15
I R Nemitz, I Gryn, N Boudet, R P Lemieux, M Goldmann, B Zappone, R G Petschek, C Rosenblatt, E Lacaze
A novel structure was observed below the smectic-A-smectic-C phase transition in a very thin open cell having an air interface above and enforced planar anchoring at the substrate below. The structure appears as periodic dark and light streaks running perpendicular to the oily streaks, which are present in the smectic-A phase [D. Coursault et al., Soft Matter, 2016, 12, 678]. These new streaks, which we call "soapy streaks", form by extending from one oily streak to the next in discrete steps, eliminating optical evidence at visible wavelengths of the oily streaks...
December 7, 2017: Soft Matter
https://www.readbyqxmd.com/read/29211785/the-role-of-the-clinical-departments-for-understanding-patient-heterogeneity-in-one-year-mortality-after-a-diagnosis-of-heart-failure-a-multilevel-analysis-of-individual-heterogeneity-for-profiling-provider-outcomes
#16
Nermin Ghith, Anne Frølich, Juan Merlo
PURPOSE: To evaluate the general contextual effect (GCE) of the hospital department on one-year mortality in Swedish and Danish patients with heart failure (HF) by applying a multilevel analysis of individual heterogeneity. METHODS: Using the Swedish patient register, we obtained data on 36,943 patients who were 45-80 years old and admitted for HF to the hospital between 2007 and 2009. From the Danish Heart Failure Database (DHFD), we obtained data on 12,001 patients with incident HF who were 18 years or older and treated at hospitals between June 2010 and June2013...
2017: PloS One
https://www.readbyqxmd.com/read/29211557/acoustophoretic-purification-of-platelets-feasibility-and-impact-on-platelet-activation-and-function
#17
Pierre Bohec, Jérémie Gachelin, Véronique Ollivier, Thibaut Mutin, Xavier Télot, Benoît Ho-Tin-Noé, Sandra Sanfilippo
Purity, limited platelet activation, and preservation of platelet function are important stakes of preparation of platelet concentrates (PC) for clinical use. In fact, contaminating red blood cells and leukocytes, as well as activated and/or poorly functional platelets in PC, represents a risk of poor efficiency and adverse side effects during platelet transfusion. Therefore, optimization of preparation and storage of PC is still an active field of research. Shear-induced platelet activation is an unwanted side effect of the hard-spin (up to 5000g) step of centrifugation-based methods currently used in blood banks to prepare PC from whole blood samples...
December 6, 2017: Platelets
https://www.readbyqxmd.com/read/29210895/characteristics-of-upper-limb-pull-power-and-power-endurance-in-japanese-female-wrestlers
#18
Tatsuki Naka, Masaaki Kanno, Shota Shidochi, Kazuhito Sakae, Norihiro Shima
This study aimed at elucidating the characteristics of upper-limb physical strength required by female wrestlers by comparing the pull power, power endurance, and number of repetitions between female wrestlers at world and national levels. The subjects were 20 female wrestlers (8 Olympic- and/or world-class and 12 national-class top-ranking individuals). The subjects assumed a lying prone (face down) position on a bench and pulled a bar from arms length until contact with underside of bench. The weight load was increased in 10-kg steps and the upper-limb pull power was measured for each lift...
November 22, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29210193/islet-damage-during-isolation-as-assessed-by-mirnas-and-the-correlation-of-mirna-levels-with-posttransplant-outcome-in-islet-autotransplantation
#19
Prathab Balaji Saravanan, Mazhar A Kanak, Charles A Chang, Carly Darden, Gumpei Yoshimatsu, Michael C Lawrence, Bashoo Naziruddin
High-quality pancreatic islets are essential for better posttransplant endocrine function in total pancreatectomy with islet autotransplantation (TPIAT), yet stress during the isolation process affects quality and yield. We analyzed islet-enriched miRNAs -375 and -200c released during isolation to assess damage and correlated the data with posttransplant endocrine function. The absolute concentration of miR-375, miR-200c, and C-peptide was measured in various islet isolation steps, including digestion, dilution, recombination, purification, and bagging, in 12 cases of TPIAT...
December 6, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/29208589/development-of-an-index-of-engagement-in-hiv-care-an-adapted-internet-based-delphi-process
#20
Mallory O Johnson, Kimberly A Koester, Troy Wood, Torsten B Neilands, Jamie L Pomeranz, Katerina A Christopoulos
BACKGROUND: Improving engagement in medical care among persons living with human immunodeficiency virus (HIV) is critical to optimizing clinical outcomes and reducing onward transmission of HIV. However, a clear conceptualization of what it means to be engaged in HIV care is lacking, and thus efforts to measure and enhance engagement in care are limited. OBJECTIVE: This paper describes the use of a modified online Delphi process of consensus building to solicit input from a range of HIV and non-HIV researchers and providers, and to integrate that input with focus group data conducted with HIV-infected patients...
December 5, 2017: JMIR Research Protocols
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