keyword
MENU ▼
Read by QxMD icon Read
search

12 step

keyword
https://www.readbyqxmd.com/read/29040674/evaluation-of-apoptotic-and-autophagic-related-protein-expressions-before-and-after-in-vitro-maturation-of-fresh-slow-frozen-and-vitrified-pre-pubertal-mouse-testicular-tissue
#1
L Dumont, F Chalmel, A Oblette, B Berby, A Rives, V Duchesne, C Rondanino, N Rives
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: In vitro maturation strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i...
October 11, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29040224/fes-assisted-cycling-improves-aerobic-capacity-and-locomotor-function-post-cva
#2
Stacey E Aaron, Catherine J VanDerwerker, Aaron E Embry, Jennifer H Newton, Samuel C K Lee, Chris M Gregory
PURPOSE: After a cerebrovascular accident (CVA) aerobic deconditioning contributes to diminished physical function. Functional Electrical Stimulation (FES)-assisted cycling is a promising exercise paradigm designed to target both aerobic capacity and locomotor function. This pilot study aimed to evaluate the effects of an FES-assisted cycling intervention on aerobic capacity and locomotor function in individuals post-CVA. METHODS: Eleven individuals with chronic (> 6 months) post-CVA hemiparesis completed an 8-week (3x/wk; 24 sessions) progressive FES-assisted cycling intervention...
October 16, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29040212/vasopressor-cumulative-dose-requirement-and-risk-of-early-death-during-septic-shock-an-analysis-from-the-episs-cohort
#3
Auguste Dargent, Maxime Nguyen, Isabelle Fournel, Abderrahmane Bourredjem, Pierre-Emmanuel Charles, Jean-Pierre Quenot
Septic shock is the primary cause of death in intensive care units, with about 20% of patients dying in the first 3 days. To design future trials focused on early mortality, we require knowledge of early indicators that can detect patients at high risk of early death from refractory septic shock.The aim of this study was to assess whether the cumulative dose of vasopressors (CDV), calculated as the cumulative dose of epinephrine + norepinephrine, is a predictor of early death (within 72 hours) attributable to refractory septic shock (EDASS)...
October 16, 2017: Shock
https://www.readbyqxmd.com/read/29036894/how-can-data-drive-policy-and-practice-in-child-welfare-making-the-link-in-canada
#4
Barbara Fallon, Joanne Filippelli, Tara Black, Nico Trocmé, Tonino Esposito
Formal university-child welfare partnerships offer a unique opportunity to begin to fill the gaps in the child welfare knowledge base and link child welfare services to the realities of practice. With resources from a knowledge mobilization grant, a formal partnership was developed between the University of Toronto, clinicians, policy analysts, and researchers from child welfare agencies across Ontario. The key objectives of the grant included: (1) enhancing the capacity of service providers to access and analyze child welfare data to inform service and policy decisions; (2) integrating clinical expertise in service and policy decisions; and (3) developing a joint research agenda addressing high-priority knowledge gaps...
October 14, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29036366/accounting-for-selectivity-bias-and-correlation-across-the-sequence-from-elevated-blood-pressure-to-hypertension-diagnosis-and-treatment
#5
Penny Gordon-Larsen, Samantha M Attard, Annie Green Howard, Barry M Popkin, Bing Zhang, Shufa Du, David K Guilkey
BACKGROUND: It is unknown whether efforts to reduce hypertension burden in countries with very high prevalence, would be more effective if directed at hypertension diagnosis vs. treatment. Most analyses do not address bias and correlation across the sequence from elevated blood pressure (BP) to hypertension diagnosis and treatment, leading to potentially misleading findings. METHODS: Using data spanning 18 years of the China Health and Nutrition Survey (n = 18,926; ages 18-75 years), we used an innovative 3-step, integrated system of equations to predict the sequence from: (i) elevated BP (systolic/diastolic BP ≥ 140/90 mm Hg) to (ii) diagnosed hypertension conditional on elevated BP, and to (iii) treatment (medication use) conditional on diagnosis, accounting for measured and unmeasured individual- and community-level confounders at each of the 3 steps...
September 26, 2017: American Journal of Hypertension
https://www.readbyqxmd.com/read/29036169/developing-a-dengue-forecast-model-using-machine-learning-a-case-study-in-china
#6
Pi Guo, Tao Liu, Qin Zhang, Li Wang, Jianpeng Xiao, Qingying Zhang, Ganfeng Luo, Zhihao Li, Jianfeng He, Yonghui Zhang, Wenjun Ma
BACKGROUND: In China, dengue remains an important public health issue with expanded areas and increased incidence recently. Accurate and timely forecasts of dengue incidence in China are still lacking. We aimed to use the state-of-the-art machine learning algorithms to develop an accurate predictive model of dengue. METHODOLOGY/PRINCIPAL FINDINGS: Weekly dengue cases, Baidu search queries and climate factors (mean temperature, relative humidity and rainfall) during 2011-2014 in Guangdong were gathered...
October 16, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29035635/effects-of-varying-the-step-duration-on-the-determination-of-lactate-thresholds-in-elite-rowers
#7
Pitre C Bourdon, Sarah M Woolford, Jonathan D Buckley
This study aimed to identify the minimum increment duration required to accurately assess two distinct lactate thresholds. Twenty-one elite rowers (12 female, 9 males) each performed 8-9 rowing tests comprising: 1) five progressive incremental tests (3, 4, 5, 7 or 10 min steps); and 2) at least three 30-min constant-intensity maximal lactate steady state (MLSS) assessments. Power output (PO) at lactate threshold 1 (LT1) was higher in the 3- and 4-min incremental tests. No other measures were different for LT1...
October 16, 2017: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/29035543/speciated-monitoring-of-gas-phase-organic-peroxy-radicals-by-chemical-ionization-mass-spectrometry-cross-reactions-between-ch3o2-ch3-co-o2-ch3-3co2-and-c-c6h11o2
#8
Barbara Noziere, David R Hanson
Organic peroxy radicals ("RO2", with R organic) are key intermediates in most oxygen-rich systems where organic compounds are oxidized (natural environment, flames, combustion engines, living organisms…). But, until recently, techniques able to monitor simultaneously and distinguish between RO2 species ("speciated" detection) have been scarce, which has limited the understanding of complex systems containing these radicals. Mass Spectrometry using proton transfer ionization has been shown previously to detect individual gas-phase RO2 separately...
October 16, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29034296/diagnosis-of-asthma-in-symptomatic-children-based-on-measures-of-lung-function-an-analysis-of-data-from-a-population-based-birth-cohort-study
#9
Clare Murray, Philip Foden, Lesley Lowe, Hannah Durrington, Adnan Custovic, Angela Simpson
BACKGROUND: Concerns have been expressed about asthma overdiagnosis. The UK National Institute of Health and Care Excellence (NICE) proposed a new diagnostic algorithm applying four lung function measures sequentially (ratio of forced expiratory volume in 1 s [FEV1] to forced vital capacity [FVC] <70%, bronchodilator reversibility ≥12%, fractional exhaled nitric oxide [FeNO] ≥35 parts per billion, and peak expiratory flow variability >20%). We aimed to assess the diagnostic value of three of the tests individually, and then test the proposed algorithm in symptomatic children...
October 2017: Lancet Child Adolesc Health
https://www.readbyqxmd.com/read/29034057/design-and-fabrication-of-a-low-cost-three-dimensional-bioprinter
#10
Colton McElheny, Daniel Hayes, Ram Devireddy
Three-dimensional (3D) bioprinting offers innovative research vectors for tissue engineering. However, commercially available bioprinting platforms can be cost prohibitive to small research facilities, especially in an academic setting. The goal is to design and fabricate a low-cost printing platform able to deliver cell-laden fluids with spatial accuracy along the X, Y, and Z axes of 0.1 mm. The bioprinter consists of three subassemblies: a base unit, a gantry, and a shuttle component. The platform utilizes four stepper motors to position along three axes and a fifth stepper motor actuating a pump...
December 2017: Journal of Medical Devices
https://www.readbyqxmd.com/read/29031833/structure-based-drug-design-for-envelope-protein-e2-uncovers-a-new-class-of-bovine-viral-diarrhea-inhibitors-that-block-virus-entry
#11
María José Pascual, Fernando Merwaiss, Emilse Leal, María Eugenia Quintana, Alejandra V Capozzo, Claudio N Cavasotto, Mariela Bollini, Diego E Alvarez
Antiviral targeting of virus envelope proteins is an effective strategy for therapeutic intervention of viral infections. Here, we took a computer-guided approach with the aim of identifying new antivirals against the envelope protein E2 of bovine viral diarrhea virus (BVDV). BVDV is an enveloped virus with an RNA genome responsible for major economic losses of the cattle industry worldwide. Based on the crystal structure of the envelope protein E2, we defined a binding site at the interface of the two most distal domains from the virus membrane and pursued a hierarchical docking-based virtual screening search to identify small-molecule ligands of E2...
October 12, 2017: Antiviral Research
https://www.readbyqxmd.com/read/29031546/influenza-virus-but-not-mers-coronavirus-circulation-in-iran-2013-2016-comparison-between-pilgrims-and-general-population
#12
Jila Yavarian, Nazanin Zahra Shafiei Jandaghi, Maryam Naseri, Peyman Hemmati, Mohhamadnasr Dadras, Mohammad Mehdi Gouya, Talat Mokhtari Azad
BACKGROUND: The pilgrimage to Mecca and Karbala bring many Muslims to a confined area. Respiratory tract infections are the most common diseases transmitted during mass gatherings in Hajj, Umrah and Karbala. The aim of this study was to determine and compare the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) and influenza virus infections among Iranian general population and pilgrims with severe acute respiratory infections (SARI) returning from Mecca and Karbala during 2013-2016...
October 11, 2017: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/29031075/discovery-of-new-indomethacin-based-analogs-with-potentially-selective-cyclooxygenase-2-inhibition-and-observed-diminishing-to-pge2-activities
#13
Shaymaa E Kassab, Mohammed A Khedr, Hamed I Ali, Mohamed M Abdalla
New ring-extended analogs of indomethacin were designed based on the structure of active binding site of both COX-1 and COX-2 isoenzymes and the interaction pattern required for selective inhibition of COX-2 to improve its selectivity against COX-2. The strategy adopted for designing the new inhibitors involved i) ring extension of indomethacin to reduce the possibility of analogs to be accommodated into the narrow hydrophobic tunnel of COX-1, ii) deletion of carboxylic acid to reduce the possibility of inhibitor to form salt bridge with Arg120 and eventually prevent COX-1 inhibition, and iii) introduction of methylsulfonyl group to increase the opportunity of the analogs to interact with the polar side pocket that's is crucial for inhibition process of COX-2...
September 28, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29030167/turnaround-time-of-whole-genome-sequencing-for-mycobacterial-identification-and-drug-susceptibility-testing-in-routine-practice
#14
Ioana D Olaru, Hemu Patel, Katharina Kranzer, Nelun Perera
OBJECTIVES: Until recently whole genome sequencing (WGS) for mycobacteria has been restricted mostly to the research setting. However, in 2017 Public Health England has implemented WGS for routine mycobacterial identification and susceptibility testing for Mycobacterium tuberculosis. Our objective was to evaluate the impact of this change on the laboratory turnaround times and availability of results. METHODS: Over the years 2016 and 2017, the period January 1(st) to April 30(th) was selected to represent before and after implementation of WGS...
October 10, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29030130/behavioural-and-objective-vestibular-assessment-in-persons-with-osteoporosis-and-osteopenia-a-preliminary-investigation
#15
Aditi Gargeshwari, Raghav Hira Jha, Niraj Kumar Singh, Prawin Kumar
INTRODUCTION: Calcium is vital for the functioning of the inner ear hair cells as well as for the neurotransmitter release that triggers the generation of a nerve impulse. A reduction in calcium level could therefore impair the peripheric vestibular functioning. However, the outcome of balance assessment has rarely been explored in cases with osteopenia and osteoporosis, the medical conditions associated with reduction in calcium levels. OBJECTIVE: The present study aimed to investigate the impact of osteopenia and osteoporosis on the outcomes of behavioural and objective vestibular assessment tests...
September 21, 2017: Brazilian Journal of Otorhinolaryngology
https://www.readbyqxmd.com/read/29029185/patient-reported-outcomes-in-newly-diagnosed-early-rheumatoid-arthritis-patients-treated-to-target-with-a-tocilizumab-or-methotrexate-based-strategy
#16
Xavier M Teitsma, Johannes W G Jacobs, Paco M J Welsing, Attila Pethö-Schramm, Michelle E A Borm, Lidy Hendriks, Natasja H A M Denissen, Jacob M van Laar, Floris P J G Lafeber, Johannes W J Bijlsma
Objective: To evaluate the effect of initiation of tocilizumab, with or without MTX, compared with MTX alone on patient-reported outcomes (PROs), in DMARD-naïve patients with early RA. Methods: In U-Act-Early, patients initiated treat-to-target step-up MTX, tocilizumab or tocilizumab plus MTX therapy. PROs assessed included the Functional Assessment of Chronic Illness Therapy-Fatigue, 36-item Short Form (SF-36), five dimensional EuroQol (EQ-5D) and the Revised Illness Perception Questionnaire...
September 23, 2017: Rheumatology
https://www.readbyqxmd.com/read/29029107/new-food-baits-for-trapping-german-cockroaches-blattella-germanica-l-dictyoptera-blattellidae
#17
Joshua C Pol, Sebastian Ibarra Jimenez, Gerhard Gries
German cockroaches (GCRs), Blattella germanica (L.) (Dictyoptera: Blattellidae), are attracted to those beer semiochemicals (e.g., ethanol) that formerly living and active yeasts have produced or otherwise formed in the brewing process. We predicted that an earlier step in the production of beer, where yeasts actively metabolize the sugar in malted barley powder (dry malt extract [DME]), is very attractive to GCRs. In laboratory experiments, a 3-component composition (3CC) comprising DME, water, and Brewer's yeast strongly attracted GCR nymphs, females, and males...
September 27, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29028205/mpigenenet-parallel-calculation-of-gene-co-expression-networks-on-multicore-clusters
#18
Jorge Gonzalez-Dominguez, Maria J Martin
In this work we present MPIGeneNet, a parallel tool that applies Pearson's correlation and Random Matrix Theory to construct gene co-expression networks. It is based on the state-of-the-art sequential tool RMTGeneNet, which provides networks with high robustness and sensitivity at the expenses of relatively long runtimes for large scale input datasets. MPIGeneNet returns the same results as RMTGeneNet but improves the memory management, reduces the I/O cost, and accelerates the two most computationally demanding steps of co-expression network construction by exploiting the compute capabilities of common multicore CPU clusters...
October 10, 2017: IEEE/ACM Transactions on Computational Biology and Bioinformatics
https://www.readbyqxmd.com/read/29027245/epac-induced-ryanodine-receptor-type-2-activation-inhibits-sodium-currents-in-atrial-and-ventricular-murine-cardiomyocytes
#19
Haseeb Valli, Shiraz Ahmad, Sujan Sriharan, Lydia D Dean, Andrew A Grace, Kamalan Jeevaratnam, Hugh R Matthews, Christopher L-H Huang
Acute RyR2 activation by exchange protein directly activated by cAMP (Epac) reversibly perturbs myocyte Ca(2+) homeostasis, slows myocardial action potential conduction, and exerts pro-arrhythmic effects. Loose patch-clamp studies, preserving in vivo extracellular and intracellular conditions, investigated Na(+) current in intact cardiomyocytes in murine atrial and ventricular preparations following Epac activation. Depolarising steps to varying test voltages activated typical voltage-dependent Na(+) currents...
October 12, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/29026627/marked-peripheral-eosinophilia-due-to-prolonged-administration-of-posaconazole
#20
Maher Alharbi, Rae-Kiran Jhinger, Terence Wuerz, Andrew Walkty
Introduction. Posaconazole is a triazole antifungal that is used in the treatment of a variety of fungal infections, as well as in the management of mucormycosis (on an off-label basis). Eosinophilia associated with exposure to azole antifungals has been described rarely in the literature. Case presentation. A 31-year-old male on peritoneal dialysis (PD) for end-stage renal disease, secondary to diabetic nephropathy, presented to hospital with abdominal pain after a trip to St Lucia. He was taken to the operating room, where the PD catheter was removed and an abdominal-wall abscess was debrided...
June 2017: JMM Case Reports
keyword
keyword
4141
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"