keyword
MENU ▼
Read by QxMD icon Read
search

UDP-Glycosyltransferases

keyword
https://www.readbyqxmd.com/read/27914321/functional-regulation-of-ginsenoside-biosynthesis-by-rna-interferences-of-a-udp-glycosyltransferase-gene-in-panax-ginseng-and-panax-quinquefolius
#1
Chao Lu, Shoujing Zhao, Guanning Wei, Huijuan Zhao, Qingling Qu
Panax ginseng (Asian ginseng) and Panax quinquefolius (American ginseng) have been used as medicinal and functional herbal remedies worldwide. Different properties of P. ginseng and P. quinquefolius were confirmed not only in clinical findings, but also at cellular and molecular levels. The major pharmacological ingredients of P. ginseng and P. quinquefolius are the triterpene saponins known as ginsenosides. The P. ginseng roots contain a higher ratio of ginsenoside Rg1:Rb1 than that in P. quinquefolius...
November 25, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27864542/involvement-of-three-putative-glucosyltransferases-from-the-ugt72-family-in-flavonol-glucoside-rhamnoside-biosynthesis-in-lotus-japonicus-seeds
#2
Qinggang Yin, Guoan Shen, Zhenzhan Chang, Yuhong Tang, Hongwen Gao, Yongzhen Pang
Flavonols are one of the largest groups of flavonoids that confer benefits for the health of plants and animals. Flavonol glycosides are the predominant flavonoids present in the model legume Lotus japonicus The molecular mechanisms underlying the biosynthesis of flavonol glycosides as yet remain unknown in L. japonicus. In the present study, we identified a total of 188 UDP-glycosyltransferases (UGTs) in L. japonicus by genome-wide searching. Notably, 12 UGTs from the UGT72 family were distributed widely among L...
November 17, 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27842501/analysis-of-transcriptional-response-to-heat-stress-in-rhazya-stricta
#3
Abdullah Y Obaid, Jamal S M Sabir, Ahmed Atef, Xuan Liu, Sherif Edris, Fotouh M El-Domyati, Mohammed Z Mutwakil, Nour O Gadalla, Nahid H Hajrah, Magdy A Al-Kordy, Neil Hall, Ahmed Bahieldin, Robert K Jansen
BACKGROUND: Climate change is predicted to be a serious threat to agriculture due to the need for crops to be able to tolerate increased heat stress. Desert plants have already adapted to high levels of heat stress so they make excellent systems for identifying genes involved in thermotolerance. Rhazya stricta is an evergreen shrub that is native to extremely hot regions across Western and South Asia, making it an excellent system for examining plant responses to heat stress. Transcriptomes of apical and mature leaves of R...
November 14, 2016: BMC Plant Biology
https://www.readbyqxmd.com/read/27821729/illuminating-a-plant-s-tissue-specific-metabolic-diversity-using-computational-metabolomics-and-information-theory
#4
Dapeng Li, Sven Heiling, Ian T Baldwin, Emmanuel Gaquerel
Secondary metabolite diversity is considered an important fitness determinant for plants' biotic and abiotic interactions in nature. This diversity can be examined in two dimensions. The first one considers metabolite diversity across plant species. A second way of looking at this diversity is by considering the tissue-specific localization of pathways underlying secondary metabolism within a plant. Although these cross-tissue metabolite variations are increasingly regarded as important readouts of tissue-level gene function and regulatory processes, they have rarely been comprehensively explored by nontargeted metabolomics...
November 22, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27819048/control-of-sexuality-by-the-sk1-encoded-udp-glycosyltransferase-of-maize
#5
Andrew P Hayward, Maria A Moreno, Thomas P Howard, Joel Hague, Kimberly Nelson, Christopher Heffelfinger, Sandra Romero, Albert P Kausch, Gaétan Glauser, Ivan F Acosta, John P Mottinger, Stephen L Dellaporta
Sex determination in maize involves the production of staminate and pistillate florets from an initially bisexual floral meristem. Pistil elimination in staminate florets requires jasmonic acid signaling, and functional pistils are protected by the action of the silkless 1 (sk1) gene. The sk1 gene was identified and found to encode a previously uncharacterized family 1 uridine diphosphate glycosyltransferase that localized to the plant peroxisomes. Constitutive expression of an sk1 transgene protected all pistils in the plant, causing complete feminization, a gain-of-function phenotype that operates by blocking the accumulation of jasmonates...
October 2016: Science Advances
https://www.readbyqxmd.com/read/27786405/overexpression-of-udp-glycosyltransferase-gene-ugt2b17-is-involved-in-chlorantraniliprole-resistance-in-plutella-xylostella-l
#6
Xiuxia Li, Bin Zhu, Xiwu Gao, Pei Liang
BACKGROUND: UDP-glycosyltransferases (UGTs) are phase II detoxification enzymes widely distributed within living organisms. Their involvement in the biotransformation of various lipophilic endogenous compounds and phytoalexins in insects has been documented. However, the roles of this enzyme family in insecticide resistance have rarely been reported. Here, the functions of UGTs in chlorantraniliprole resistance in Plutella xylostella were investigated. RESULTS: Treatment with sulfinpyrazone and 5-nitrouracil (both inhibitors of UGT enzymes) significantly increased the toxicity of chlorantraniliprole against the third instar larvae of P...
October 27, 2016: Pest Management Science
https://www.readbyqxmd.com/read/27757117/metabolic-and-molecular-changes-of-the-phenylpropanoid-pathway-in-tomato-solanum-lycopersicum-lines-carrying-different-solanum-pennellii-wild-chromosomal-regions
#7
Maria Manuela Rigano, Assunta Raiola, Teresa Docimo, Valentino Ruggieri, Roberta Calafiore, Paola Vitaglione, Rosalia Ferracane, Luigi Frusciante, Amalia Barone
Solanum lycopersicum represents an important dietary source of bioactive compounds including the antioxidants flavonoids and phenolic acids. We previously identified two genotypes (IL7-3 and IL12-4) carrying loci from the wild species Solanum pennellii, which increased antioxidants in the fruit. Successively, these lines were crossed and two genotypes carrying both introgressions at the homozygous condition (DHO88 and DHO88-SL) were selected. The amount of total antioxidant compounds was increased in DHOs compared to both ILs and the control genotype M82...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27747895/the-arabidopsis-ugt87a2-a-stress-inducible-family-1-glycosyltransferase-is-involved-in-the-plant-adaptation-to-abiotic-stresses
#8
Pan Li, Yan-Jie Li, Bo Wang, Hui-Min Yu, Qin Li, Bing-Kai Hou
Glycosyltransferase (GT) family-1, the biggest GT family in plants, typically participates in modification of small molecules and affects many aspects during plant development. In Arabidopsis thaliana, although some UDP glycosyltransferases (UGTs) of family-1 have been functionally characterized, functions of most the UGTs remain unknown or fragmentary. Here, we report data for the Arabidopsis UGT87A2, a stress-regulated GT. We found that UGT87A2 could be dramatically induced by salinity, osmotic stress, drought and ABA...
October 17, 2016: Physiologia Plantarum
https://www.readbyqxmd.com/read/27746309/fungal-elicitors-enhance-ginsenosides-biosynthesis-expression-of-functional-genes-as-well-as-signal-molecules-accumulation-in-adventitious-roots-of-panax-ginseng-c-a-mey
#9
Jinxin Li, Shujie Liu, Juan Wang, Jing Li, Dahui Liu, Jianli Li, Wenyuan Gao
In this work, we selected three fungi strains (Aspergillus niger, Aspergillus flavus and Aspergillus oryzae) as elicitors prepared from mycelium or fermentation broth to improve ginsenosides production in adventitious roots culture. The results showed that ginsenosides production (29.90±4.67mgg(-1)) was significantly enhanced upon elicitation with 200mgL(-1)A. niger elicitor prepared from mycelium, which was 3.52-fold of untreated group. HPLC-ESI-MS(n) analysis was performed, showing that ginsenoside Rb3 was present after treatment with the A...
October 13, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27721120/genome-wide-analysis-of-family-1-udp-glycosyltransferases-in-soybean-confirms-their-abundance-and-varied-expression-during-seed-development
#10
Hafiz Mamoon Rehman, Muhammad Amjad Nawaz, Le Bao, Zahid Hussain Shah, Jae-Min Lee, Muhammad Qadir Ahmad, Gyuhwa Chung, Seung Hwan Yang
Family-1 UDP-glycosyltransferases (EC 2.4.1.x; UGTs) are enzymes that glycosylate aglycones into glycoside-associated compounds with improved transport and water solubility. This glycosylation mechanism is vital to plant functions, such as regulation of hormonal homeostasis, growth and development, xenobiotic detoxification, stress response, and biosynthesis of secondary metabolites. Here, we report a genome-wide analysis of soybean that identified 149 putative UGTs based on 44 conserved plant secondary product glycosyl-transferase (PSPG) motif amino acid sequences...
September 22, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/27638608/substrate-reduction-therapy-for-krabbe-s-disease
#11
REVIEW
Scott A Sands, Steven M LeVine
Krabbe's disease (KD) is a lysosomal storage disorder in which galactosylceramide, a major glycosphingolipid of myelin, and psychosine (galactose-sphingosine) cannot be adequately metabolized because of a deficiency in galactosylceramidase. Substrate reduction therapy (SRT) has been tested in preclinical studies. The premise of SRT is to reduce the synthesis of substrates that are not adequately digested so that the substrate burden is lowered, resulting in less accumulation of unmetabolized material. SRT is used for Gaucher's disease, in which inhibitors of the terminal biosynthetic step are used...
November 2016: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/27621739/transcriptome-analysis-of-pepper-capsicum-annuum-revealed-a-role-of-24-epibrassinolide-in-response-to-chilling
#12
Jie Li, Ping Yang, Jungen Kang, Yantai Gan, Jihua Yu, Alejandro Calderón-Urrea, Jian Lyu, Guobin Zhang, Zhi Feng, Jianming Xie
Brassinosteroids (BRs) have positive effects on many processes during plant growth, development, and various abiotic stress responses. However, little information is available regarding the global gene expression of BRs in response to chilling stress in pepper. In this study, we used RNA sequencing to determine the molecular roles of 24-epibrassinolide (EBR) during a chilling stress response. There were 39,829 transcripts, and, among them, 656 were differently-expressed genes (DEGs) following EBR treatment (Chill+EBR) compared with the control (Chill only), including 335 up-regulated and 321 down-regulated DEGs...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27599367/the-arabidopsis-udp-glycosyltransferases-ugt79b2-and-ugt79b3-contribute-to-cold-salt-and-drought-stress-tolerance-via-modulating-anthocyanin-accumulation
#13
Pan Li, Yan-Jie Li, Feng-Ju Zhang, Gui-Zhi Zhang, Xiao-Yi Jiang, Hui-Min Yu, Bing-Kai Hou
The plant family 1 UDP-glycosyltransferases (UGTs) are the biggest GT family in plants, which are responsible for transferring sugar moieties onto a variety of small molecules, and control many metabolic processes; however, their physiological significance in planta is largely unknown. Here, we revealed that two Arabidopsis glycosyltransferase genes, UGT79B2 and UGT79B3, could be strongly induced by various abiotic stresses, including cold, salt and drought stresses. Overexpression of UGT79B2/B3 significantly enhanced plant tolerance to low temperatures as well as drought and salt stresses, whereas the ugt79b2/b3 double mutants generated by RNAi (RNA interference) and CRISPR-Cas9 strategies were more susceptible to adverse conditions...
September 6, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27578568/-biosynthesis-of-a-new-psoralidin-glucoside-by-enzymatic-glycosylation
#14
Jing Li, Nan Li, Yu-Ru Zhao, Yi-Qun Dai, Qiang Huo, Tao Ma, Hong-Mei Li, Cheng-Zhu Wu
OBJECTIVE: To modify the structure of psoralidin using in vitro enzymatic glycosylation to improve its water solubility and stability. METHODS: A new psoralidin glucoside (1) was obtained by enzymatic glycosylation using a UDP- glycosyltransferase. The chemical structure of compound 1 was elucidated by HR-ESI-MS and nuclear magnetic resonance (NMR) analysis. The high-performance liquid chromatography (HPLC) peaks were integrated and sample solution concentrations were calculated...
August 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27507791/glucose-or-altered-ceramide-biosynthesis-mediate-oxygen-deprivation-sensitivity-through-novel-pathways-revealed-by-transcriptome-analysis-in-caenorhabditis-elegans
#15
Mary L Ladage, Skylar D King, David J Burks, Daniel L Quan, Anastacia M Garcia, Rajeev K Azad, Pamela A Padilla
Individuals with type 2 diabetes display metabolic abnormalities, such as hyperglycemia, increased free fatty acids, insulin resistance, and altered ceramide levels, that contribute to vascular dysfunctions and compromised oxygen delivery. Caenorhabditis elegans fed a glucose-supplemented diet or with altered ceramide metabolism, due to a hyl-2 mutation, are sensitive to oxygen deprivation (anoxia). Our experiments showed that the combination of these factors further decreased the anoxia survival. RNA-sequencing analysis was performed to assess how a glucose-supplemented diet and/or a hyl-2 mutation altered the transcriptome...
October 13, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27504115/comprehensive-analysis-of-the-triterpenoid-saponins-biosynthetic-pathway-in-anemone-flaccida-by-transcriptome-and-proteome-profiling
#16
Chuansong Zhan, Xiaohua Li, Zeying Zhao, Tewu Yang, Xuekui Wang, Biaobiao Luo, Qiyun Zhang, Yanru Hu, Xuebo Hu
BACKGROUND: Anemone flaccida Fr. Shmidt (Ranunculaceae), commonly known as 'Di Wu' in China, is a perennial herb with limited distribution. The rhizome of A. flaccida has long been used to treat arthritis as a tradition in China. Studies disclosed that the plant contains a rich source of triterpenoid saponins. However, little is known about triterpenoid saponins biosynthesis in A. flaccida. RESULTS: In this study, we conducted the tandem transcriptome and proteome profiling of a non-model medicinal plant, A...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27491884/estimation-of-bisphenol-a-human-toxicity-by-3d-cell-culture-arrays-high-throughput-alternatives-to-animal-tests
#17
Dong Woo Lee, Woo-Yeon Oh, Sang Hyun Yi, Bosung Ku, Moo-Yeal Lee, Yoon Hee Cho, Mihi Yang
Bisphenol A (BPA) has been widely used for manufacturing polycarbonate plastics and epoxy resins and has been extensively tested in animals to predict human toxicity. In order to reduce the use of animals for toxicity assessment and provide further accurate information on BPA toxicity in humans, we encapsulated Hep3B human hepatoma cells in alginate and cultured them in three dimensions (3D) on a micropillar chip coupled to a panel of metabolic enzymes on a microwell chip. As a result, we were able to assess the toxicity of BPA under various metabolic enzyme conditions using a high-throughput and micro assay; sample volumes were nearly 2,000 times less than that required for a 96-well plate...
September 30, 2016: Toxicology Letters
https://www.readbyqxmd.com/read/27411741/identification-of-a-residue-responsible-for-udp-sugar-donor-selectivity-of-a-dihydroxybenzoic-acid-glycosyltransferase-from-arabidopsis-natural-accessions
#18
Han-Yi Chen, Xu Li
UDP glycosyltransferase plays a major role in the diversity and reactivity of plant specialized metabolites by catalyzing the transfer of the sugar moiety from activated UDP-sugars to various acceptors. Arabidopsis UGT89A2 was previously identified from a genome-wide association study as a key factor that affects the differential accumulation of dihydroxybenzoic acid (DHBA) glycosides in distinct Arabidopsis natural accessions, including Col-0 and C24. The in vitro enzyme assays indicate that these distinct metabolic phenotypes reflect the divergence of UGT89A2 enzyme properties in the Col-0 and C24 accessions...
July 14, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27391785/expression-patterns-of-three-ugt-genes-in-different-chemotype-safflower-lines-and-under-meja-stimulus-revealed-their-potential-role-in-flavonoid-biosynthesis
#19
Dan-Dan Guo, Fei Liu, Yan-Hua Tu, Bei-Xuan He, Yue Gao, Mei-Li Guo
Safflower (Carthamus tinctorius L.) has received a significant amount of attention as a medicinal plant in China. Flavonoids are the dominant active medical compounds. UDP-glycosyltransferase plays an essential role in the biosynthesis and storage of flavonoids in safflower. In this study, 45 UGT unigenes were screened from our transcriptomic database of safflower. Among them, 27 UGT unigenes were predicted to own a complete open reading frame with various pI and Mw. The phylogenetic tree showed that CtUGT3 and CtUGT16 were classified under the UGT71 subfamily involved in metabolite process, whereas CtUGT25 has high identities with PoUGT both catalyzing the glycosylation of flavonoids and belonging to the UGT90 subfamily...
2016: PloS One
https://www.readbyqxmd.com/read/27378621/screening-of-recombinant-glycosyltransferases-reveals-the-broad-acceptor-specificity-of-stevia-ugt-76g1
#20
Griet Dewitte, Maarten Walmagh, Margo Diricks, Alexander Lepak, Alexander Gutmann, Bernd Nidetzky, Tom Desmet
UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their potential application in glycosylation processes. Recombinant expression in E. coli provided sufficient amounts of enzyme for the in-depth characterization of the salicylic acid UGT from Capsella rubella (UGT-SACr) and the stevia UGT from Stevia rebaudiana (UGT-76G1Sr)...
September 10, 2016: Journal of Biotechnology
keyword
keyword
41409
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"