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Platelet Proteomics

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https://www.readbyqxmd.com/read/29665821/plasma-proteomic-analysis-reveals-altered-protein-abundances-in-cardiovascular-disease
#1
Vasiliki Lygirou, Agnieszka Latosinska, Manousos Makridakis, William Mullen, Christian Delles, Joost P Schanstra, Jerome Zoidakis, Burkert Pieske, Harald Mischak, Antonia Vlahou
BACKGROUND: Cardiovascular disease (CVD) describes the pathological conditions of the heart and blood vessels. Despite the large number of studies on CVD and its etiology, its key modulators remain largely unknown. To this end, we performed a comprehensive proteomic analysis of blood plasma, with the scope to identify disease-associated changes after placing them in the context of existing knowledge, and generate a well characterized dataset for further use in CVD multi-omics integrative analysis...
April 17, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29611199/preliminary-study-of-protein-changes-in-trisomy-21-fetus-by-proteomics-analysis-in-amniocyte
#2
Hui Liu, He Wang, Zhu Hongmei, Haixia Zhang, Shanling Liu
OBJECTIVE: To discover the candidate biomarker proteins of trisomy 21 in amniocytes. METHODS: Amniocentesis was performed to collect AF from women who underwent prenatal diagnosis due to high risk of T21 at 18th -21st week of gestation. Amniocyte samples were collected and karyotyping analysis was used to confirm the chromosomal status (Eighteen samples of T21 amniocytes and twenty samples of chromosomally normal ones). Then backup samples for cytogenetic test were used in this study...
April 2, 2018: Prenatal Diagnosis
https://www.readbyqxmd.com/read/29603091/platelet-proteomic-analysis-revealed-differential-pattern-of-cytoskeletal-and-immune-related-proteins-at-early-stages-of-alzheimer-s-disease
#3
Marta González-Sánchez, Teresa Díaz, Consuelo Pascual, Desiree Antequera, Alejandro Herrero-San Martín, Sara Llamas-Velasco, Alberto Villarejo-Galende, Fernando Bartolome, Eva Carro
Platelets are considered a good model system to study a number of elements associated with neuronal pathways as they share biochemical similarities. Platelets represent the major source of amyloid-β (Aβ) in blood contributing to the Aβ accumulation in the brain parenchyma and vasculature. Peripheral blood platelet alterations including cytoskeletal abnormalities, abnormal cytoplasmic calcium fluxes or increased oxidative stress levels have been related to Alzheimer's disease (AD) pathology. Therefore, platelets can be considered a peripheral model to study metabolic mechanisms occurring in AD...
March 30, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29595346/a-proteomic-approach-reveals-the-variation-in-human-platelet-protein-composition-after-storage-at-different-temperatures
#4
Shichun Wang, Tianlun Jiang, Yahan Fan, Shuming Zhao
Cryopreservation can slow down the metabolism and decrease the risk of bacterial contamination. But, chilled platelets (PLTs) show a reduced period in circulation due to the rapid clearance by hepatic cells or spleen macrophages after transfusion. The deleterious changes that PLTs undergo are mainly considered the result of PLT protein variation. However, the basis for proteomic variation of stored PLTs remains poorly understood. Besides count, activation markers (CD62P and Annexin V), and aggregation, we used quantitative mass spectrometry to create the first comprehensive and quantitative human PLT proteome of samples stored at different temperatures (22°C, 10°C and -80°C)...
March 29, 2018: Platelets
https://www.readbyqxmd.com/read/29578674/proteomic-characterization-of-the-neural-ectoderm-fated-cell-clones-in-the-xenopus-laevis-embryo-by-high-resolution-mass-spectrometry
#5
Aparna B Baxi, Camille Lombard-Banek, Sally A Moody, Peter Nemes
The molecular program by which embryonic ectoderm is induced to form neural tissue is essential to understanding normal and impaired development of the central nervous system. Xenopus has been a powerful vertebrate model in which to elucidate this process. However, abundant vitellogenin (yolk) proteins in cells of the early Xenopus embryo interfere with protein detection by high-resolution mass spectrometry (HRMS), the technology of choice for identifying these gene products. Here, we systematically evaluated strategies of bottom-up proteomics to enhance proteomic detection from the neural ectoderm (NE) of X...
March 26, 2018: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29564590/proteomic-analysis-of-the-antidepressant-effects-of-shen-zhi-ling-in-depressed-patients-identification-of-proteins-associated-with-platelet-activation-and-lipid-metabolism
#6
Chao Chen, Yuan Hu, Xian-Zhe Dong, Xiao-Jiang Zhou, Li-Hua Mu, Ping Liu
Shen-Zhi-Ling (SZL) is a Chinese medicine formulated from a Kai-Xin-San decoction that is commonly used to treat depression caused by dual deficiencies in the heart and spleen. However, the underlying mechanisms remain unclear. We investigated biological changes in depression patients (DPs) exhibiting antidepressant responses to SZL treatment using proteomic techniques. We performed label-free quantitative proteomic analysis and liquid chromatography-tandem mass spectrometry to discover and examine altered proteins involved in depression and antidepressant treatment...
March 21, 2018: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/29553857/a-comprehensive-proteomics-study-on-platelet-concentrates-platelet-proteome-storage-time-and-mirasol-pathogen-reduction-technology
#7
Vishal Salunkhe, Iris M De Cuyper, Petros Papadopoulos, Pieter F van der Meer, Brunette B Daal, María Villa-Fajardo, Dirk de Korte, Timo K van den Berg, Laura Gutiérrez
Platelet concentrates (PCs) represent a blood transfusion product with a major concern for safety as their storage temperature (20-24°C) allows bacterial growth, and their maximum storage time period (less than a week) precludes complete microbiological testing. Pathogen inactivation technologies (PITs) provide an additional layer of safety to the blood transfusion products from known and unknown pathogens such as bacteria, viruses, and parasites. In this context, PITs, such as Mirasol Pathogen Reduction Technology (PRT), have been developed and are implemented in many countries...
March 19, 2018: Platelets
https://www.readbyqxmd.com/read/29550521/effects-of-the-no-soluble-guanylate-cyclase-cgmp-system-on-the-functions-of-human-platelets
#8
REVIEW
Stephanie Makhoul, Elena Walter, Oliver Pagel, Ulrich Walter, Albert Sickmann, Stepan Gambaryan, Albert Smolenski, René P Zahedi, Kerstin Jurk
Platelets are circulating sentinels of vascular integrity and are activated, inhibited, or modulated by multiple hormones, vasoactive substances or drugs. Endothelium- or drug-derived NO strongly inhibits platelet activation via activation of the soluble guanylate cyclase (sGC) and cGMP elevation, often in synergy with cAMP-elevation by prostacyclin. However, the molecular mechanisms and diversity of cGMP effects in platelets are poorly understood and sometimes controversial. Recently, we established the quantitative human platelet proteome, the iloprost/prostacyclin/cAMP/protein kinase A (PKA)-regulated phosphoproteome, and the interactions of the ADP- and iloprost/prostacyclin-affected phosphoproteome...
March 14, 2018: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29549767/influence-of-dynamic-flow-conditions-on-adsorbed-plasma-protein-corona-and-surface-induced-thrombus-generation-on-antifouling-brushes
#9
Kai Yu, Paula Andruschak, Han Hung Yeh, Dana Grecov, Jayachandran N Kizhakkedathu
The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis. In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions...
March 8, 2018: Biomaterials
https://www.readbyqxmd.com/read/29541006/change-of-ranibizumab-induced-human-vitreous-protein-profile-in-patients-with-proliferative-diabetic-retinopathy-based-on-proteomics-analysis
#10
Chen Zou, Changjing Han, Minjie Zhao, Jingjing Yu, Lin Bai, Yuan Yao, Shuaixin Gao, Hui Cao, Zhi Zheng
Background: Preoperative treatment of anti-vascular endothelial growth factor (VEGF) agents is extensively used in proliferative diabetic retinopathy (PDR), but the molecular mechanism is not fully understood. The objective of this research is to observe change of protein profile induced by ranibizumab (an anti-VEGF agent) in vitreous humor from PDR patients and reveal the effects of anti-VEGF treatment on PDR. Methods: A proteomic method was used to identify differentially expressed proteins in vitreous humor...
2018: Clinical Proteomics
https://www.readbyqxmd.com/read/29536409/aqueous-humor-protein-dysregulation-in-primary-angle-closure-glaucoma
#11
Sunil S Adav, Jin Wei, Jingru Qian, Nicola Yi'An Gan, Leonard W L Yip, Siu Kwan Sze
PURPOSE: Primary angle-closure glaucoma (PACG) is associated with increased intraocular pressure, optic nerve damage, and progressive vision loss, but the molecular mechanism that underpins retinal ganglion neuropathy in PACG remains poorly understood. To better understand the pathogenesis of human PACG, we performed the first comprehensive proteomic analysis of aqueous humor (AH) samples from PACG patients and matched control donors to study pathogenic alteration in AH composition in disease...
March 13, 2018: International Ophthalmology
https://www.readbyqxmd.com/read/29522771/the-contribution-of-chronic-intermittent-hypoxia-to-osahs-from-the-perspective-of-serum-extracellular-microvesicle-proteins
#12
REVIEW
Huina Zhang, Fan Yang, Yichen Guo, Li Wang, Fang Fang, Hao Wu, Shaoping Nie, Yifan Wang, Man-Lung Fung, Yu Huang, Haiteng Deng, Yanwen Qin, Xinliang Ma, Yongxiang Wei
OBJECTIVE: Obstructive sleep apnea hypopnea syndrome (OSAHS) is an independent risk factor for many clinical complications. However, how OSAHS cause multiple organ injury and initiate inter-organ communication remains unclear. Moreover, despite it is well-recognized that chronic intermittent hypoxia (CIH) is a main feature of OSAHS, specific contribution of CIH to overall OSAHS-initiated pathological complications remains unclear. This study aimed to use an unbiased proteomic approach to determine whether OSAHS alters protein profiles of serum extracellular microvesicles (SEMVs) and how CIH contributes to such alterations...
March 6, 2018: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/29518939/quantitative-proteomic-approach-targeted-to-fibrinogen-%C3%AE-chain-in-tissue-gastric-carcinoma
#13
Ombretta Repetto, Stefania Maiero, Raffaella Magris, Gianmaria Miolo, Maria Rita Cozzi, Agostino Steffan, Vincenzo Canzonieri, Renato Cannizzaro, Valli De Re
Elevated plasma fibrinogen levels and tumor progression in patients with gastric cancer (GC) have been largely reported. However, distinct fibrinogen chains and domains have different effects on coagulation, inflammation, and angiogenesis. The aim of this study was to characterize fibrinogen β chain (FGB) in GC tissues. Retrospectively we analyzed the data of matched pairs of normal (N) and malignant tissues (T) of 28 consecutive patients with GC at diagnosis by combining one- and two-dimensional electrophoresis (1DE and 2DE) with immunoblotting and mass spectrometry together with two-dimensional difference in gel electrophoresis (2D-DIGE)...
March 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29510447/proteomic-analysis-reveals-a-strong-association-of-%C3%AE-catenin-with-cadherin-adherens-junctions-in-resting-human-platelets
#14
Patricia B Maguire, Tim Donlon, Martin Parsons, Kieran Wynne, Eugene Dillon, Fionnuala Ní Áinle, Paulina B Szklanna
We previously demonstrated that the WNT/β-catenin pathway is present and active in platelets and established that the canonical WNT ligand, WNT-3a, suppresses platelet adhesion and activation. In nucleated cells, β-catenin, the key downstream effector of this pathway, is a dual function protein, regulating the coordination of gene transcription and cell-cell adhesion. The specific role of β-catenin in the anucleate platelet however remains elusive. Here, we performed a label-free quantitative proteomic analysis of β-catenin immunoprecipitates from human platelets and identified 9 co-immunoprecipitating proteins...
March 6, 2018: Proteomics
https://www.readbyqxmd.com/read/29506054/exocytosis-protein-doc2b-as-a-biomarker-of-type-1-diabetes
#15
Arianne Aslamy, Eunjin Oh, Miwon Ahn, Abu Saleh Md Moin, Mariann Chang, Molly Duncan, Jeannette Hacker-Stratton, Mohamed El-Shahawy, Fouad Kandeel, Linda A DiMeglio, Debbie C Thurmond
Context: Efforts to preserve β-cell mass in the preclinical stages of type 1 diabetes (T1D) are limited by few blood-derived biomarkers of β-cell destruction. Objective: Platelets are proposed sources of blood-derived biomarkers for a variety of diseases, and they show distinct proteomic changes in T1D. Thus, we investigated changes in the exocytosis protein, double C2 domain protein-β (DOC2B) in platelets and islets from T1D humans and pre-diabetic NOD mice...
March 1, 2018: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29505751/dexamethasone-intravitreal-implant-downregulates-pdgfr-%C3%AE-and-upregulates-caveolin-1-in-experimental-branch-retinal-vein-occlusion
#16
Lasse Jørgensen Cehofski, Anders Kruse, Sigriður Olga Magnusdottir, Alexander Nørgård Alsing, Svend Kirkeby, Bent Honoré, Henrik Vorum
A dexamethasone intravitreal implant (OZURDEX) provides an effective treatment of inflammation secondary to branch retinal vein occlusion (BRVO). Retinal proteome changes which mediate the beneficial effects of the implant remain poorly understood. To study retinal proteome changes in BRVO following an intervention with a dexamethasone (DEX) implant this study combined an experimental model of BRVO with proteomic techniques. In six Danish Landrace pigs experimental BRVO was induced in both eyes using argon laser...
March 2, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29449599/red-blood-cells-are-dynamic-reservoirs-of-cytokines
#17
Elisabeth Karsten, Edmond Breen, Benjamin R Herbert
Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous studies have identified, but not quantified, a small number of chemokines associated with RBCs. We isolated RBCs from healthy volunteers and quantified of a panel of 48 cytokines, chemokines, and growth factors in the lysate, cytosol, and conditioned media of these cells using Luminex® technology...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29432918/exploration-of-the-platelet-proteome-in-patients-with-early-stage-cancer
#18
Siamack Sabrkhany, Marijke J E Kuijpers, Jaco C Knol, Steven W M Olde Damink, Anne-Marie C Dingemans, Henk M Verheul, Sander R Piersma, Thang V Pham, Arjan W Griffioen, Mirjam G A Oude Egbrink, Connie R Jimenez
Platelets play an important role in tumor growth and, at the same time, platelet characteristics are affected by cancer presence. Therefore, we investigated whether the platelet proteome harbors differentially expressed proteins associated with early-stage cancer. For this proof-of-concept study, patients with early-stage lung (n = 8) or head of pancreas cancer (n = 4) were included, as were healthy sex- and age-matched controls for both subgroups. Blood samples were collected from controls and from patients before surgery...
February 9, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29427073/dissecting-the-biochemical-architecture-and-morphological-release-pathways-of-the-human-platelet-extracellular-vesiculome
#19
Silvia H De Paoli, Tseday Z Tegegn, Oumsalama K Elhelu, Michael B Strader, Mehulkumar Patel, Lukas L Diduch, Ivan D Tarandovskiy, Yong Wu, Jiwen Zheng, Mikhail V Ovanesov, Abdu Alayash, Jan Simak
Platelet extracellular vesicles (PEVs) have emerged as potential mediators in intercellular communication. PEVs exhibit several activities with pathophysiological importance and may serve as diagnostic biomarkers. Here, imaging and analytical techniques were employed to unveil morphological pathways of the release, structure, composition, and surface properties of PEVs derived from human platelets (PLTs) activated with the thrombin receptor activating peptide (TRAP). Based on extensive electron microscopy analysis, we propose four morphological pathways for PEVs release from TRAP-activated PLTs: (1) plasma membrane budding, (2) extrusion of multivesicular α-granules and cytoplasmic vacuoles, (3) plasma membrane blistering and (4) "pearling" of PLT pseudopodia...
February 9, 2018: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29421274/autonomous-role-of-wiskott-aldrich-syndrome-platelet-deficiency-in-inducing-autoimmunity-and-inflammation
#20
Lucia Sereni, Maria Carmina Castiello, Francesco Marangoni, Achille Anselmo, Dario di Silvestre, Sara Motta, Elena Draghici, Stefano Mantero, Adrian J Thrasher, Silvia Giliani, Alessandro Aiuti, Pierluigi Mauri, Luigi D Notarangelo, Marita Bosticardo, Anna Villa
BACKGROUND: Wiskott-Aldrich syndrome (WAS) is an X-linked immunodeficiency characterized by eczema, infections, and susceptibility to autoimmunity and malignancies. Thrombocytopenia is a constant finding, but its pathogenesis remains elusive. OBJECTIVE: To dissect the basis of the WAS platelet defect, we used a novel conditional mouse model (CoWas) lacking Wiskott-Aldrich syndrome protein (WASp) only in the megakaryocytic lineage in the presence of a normal immunologic environment, and in parallel we analyzed samples obtained from patients with WAS...
February 6, 2018: Journal of Allergy and Clinical Immunology
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