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Platelet Proteomics

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https://www.readbyqxmd.com/read/27911324/proteomics-analysis-of-blood-serums-from-alzheimer-s-disease-patients-using-itraq-labeling-technology
#1
Liming Shen, Liping Liao, Cheng Chen, Yi Guo, Dalin Song, Yong Wang, Youjiao Chen, Kaoyuan Zhang, Ming Ying, Shuiming Li, Qiong Liu, Jiazuan Ni
Alzheimer' disease (AD) is the most common form of dementia affecting up to 6% of the population over the age of 65. In order to discover differentially expressed proteins that might serve as potential biomarkers, the serums from AD patients and healthy controls were compared and analyzed using the proteomics approach of isobaric tagging for relative and absolute quantitation (iTRAQ). For the first time, AD biomarkers in serums are investigated in the Han Chinese population using iTRAQ labeled proteomics strategy...
December 1, 2016: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/27905125/gadd45%C3%AE-is-involved-in-the-apoptosis-of-lymphocytes-induced-by-riboflavin-and-ultraviolet-light
#2
Peng Yang, Huiqin Wen, Tao Zhong, Hailiang Hu, Bangqiang Zhu, Kang Xia, Mo Xu, Maohong Bian
BACKGROUND: Riboflavin plus ultraviolet (UV) pathogen reduction technology (RF-PRT) is an effective method for inactivating the residual white blood cells (WBCs) in blood components. The RF-PRT system for platelets is known to activate many signaling pathways, including p38 and NF-κB. Nevertheless, proteomic studies in WBCs after riboflavin plus UV treatment requires further analysis. STUDY DESIGN AND METHODS: ABO/D-matched lymphocytes were pooled, split, and treated with RF-PRT or UV light or left untreated...
December 1, 2016: Transfusion
https://www.readbyqxmd.com/read/27879288/multi-omics-analysis-of-serum-samples-demonstrates-reprogramming-of-organ-functions-via-systemic-calcium-mobilization-and-platelet-activation-in-metastatic-melanoma
#3
Besnik Muqaku, Martin Eisninger, Samuel M Meier, Ammar Tahir, Tobias Prokop, Sebastian Haferkamp, Astrid Slany, Albrecht Reichle, Christopher Gerner
Pathophysiologies of cancer-associated syndroms such as cachexia are poorly understood and no routine biomarkers have been established, yet. Using shotgun proteomics, known marker molecules including PMEL, CRP, SAA and CSPG4 were found deregulated in patients with metastatic melanoma. Targeted analysis of 58 selected proteins with multiple reaction monitoring was applied for independent data verification. In three patients, two of which suffered from cachexia, a tissue damage signature was determined, consisting of nine proteins, PLTP, CD14, TIMP1, S10A8, S10A9, GP1BA, PTPRJ, CD44 and C4A, as well as increased levels of glycine and asparagine, and decreased levels of polyunsaturated phosphatidylcholine concentrations, as determined by targeted metabolomics...
November 22, 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27865782/application-of-nano-lc-maldi-tof-tof-ms-for-proteomic-analysis-of-microvesicles
#4
Joanna Kasprzyk, Ewa Stępień, Wojciech Piekoszewski
Activated platelets and platelet derived microvesicles (PMVs) emerged recently to be promising biomarkers. There is no universal procedure to carry out the proteomic analysis on microvesicles. In this study we proposed a nano-liquid chromatography (nano-LC) technique coupled off-line with a spectrometric measurement MALDI-TOF-MS/MS as a throughput and time-saving procedure. In this study we developed a simplified method to evaluate the protein composition of platelet organelles and PMVs. Platelet-rich plasma (PRP) was collected from healthy donors...
November 16, 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27865523/alterations-in-high-density-lipoprotein-proteome-and-function-associated-with-persistent-organic-pollutants
#5
Stefan A Ljunggren, Ingela Helmfrid, Ulf Norinder, Mats Fredriksson, Gun Wingren, Helen Karlsson, Mats Lindahl
There is a growing body of evidence that persistent organic pollutants (POPs) may increase the risk for cardiovascular disease (CVD), but the mechanisms remain unclear. High-density lipoprotein (HDL) acts protective against CVD by different processes, and we have earlier found that HDL from subjects with CVD contains higher levels of POPs than healthy controls. In the present study, we have expanded analyses on the same individuals living in a contaminated community and investigated the relationship between the HDL POP levels and protein composition/function...
November 16, 2016: Environment International
https://www.readbyqxmd.com/read/27815141/the-role-of-micrornas-in-platelet-biology-during-storage
#6
REVIEW
Yuzhong Yan, Jingjun Zhang, Qi Zhang, Yanping Chen, Xinfang Zhu, Rong Xia
Platelet storage lesions seriously affect the quality of stored platelets, even causing them to be ineffective in vivo after transfusion. Past research have been focused on what mechanism(s) cause the formation of storage lesions. One proposed mechanism is microRNAs (miRNAs)-based molecular regulation of the platelet mRNAs that are relevant to the storage lesion. Platelets continue to translate proteins from mRNA while in a storage environment. A strong correlation exists between the platelet transcriptome and its subsequent proteomic profile, which supports de novo platelet translational capabilities...
October 27, 2016: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/27738094/robust-label-free-quantitative-profiling-of-circulating-plasma-microparticle-mp-associated-proteins
#7
Sophie Braga-Lagache, Natasha Buchs, Mircea-Ioan Iacovache, Benoît Zuber, Christopher Benjamin Jackson, Manfred Heller
Cells of the vascular system release spherical vesicles, called microparticles, in the size range of 0.1-1 μm induced by a variety of stress factors resulting in variable concentrations between health and disease. Furthermore, microparticles have intercellular communication/signaling properties and interfere with inflammation and coagulation pathways. Today's most used analytical technology for microparticle characterization, flow cytometry, is lacking sensitivity and specificity, which might have led to the publication of contradicting results in the past...
December 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27718750/platelet-proteomics-applied-to-the-search-for-novel-antiplatelet-therapeutic-targets
#8
Irene Izquierdo, Ángel García
Unwanted platelet activation is associated with numerous diseases, mainly thrombosis-related. In this context, proteomics has emerged as a novel tool with potential for drug target discovery and to scrutinize the effects of antiplatelet drugs. Areas covered: The present review presents the main findings of platelet proteomic studies to date in the context of drug target discovery and perspectives for the future ahead. It includes data and evidences obtained from literature searches on PubMed as well as commentaries derived from the authors' experience and opinions...
October 8, 2016: Expert Review of Proteomics
https://www.readbyqxmd.com/read/27716777/thioredoxin-inhibitors-attenuate-platelet-function-and-thrombus-formation
#9
Clive Metcalfe, Anjana Ramasubramoni, Giordano Pula, Matthew T Harper, Stuart J Mundell, Carmen H Coxon
Thioredoxin (Trx) is an oxidoreductase with important physiological function. Imbalances in the NADPH/thioredoxin reductase/thioredoxin system are associated with a number of pathologies, particularly cancer, and a number of clinical trials for thioredoxin and thioredoxin reductase inhibitors have been carried out or are underway. Due to the emerging role and importance of oxidoreductases for haemostasis and the current interest in developing inhibitors for clinical use, we thought it pertinent to assess whether inhibition of the NADPH/thioredoxin reductase/thioredoxin system affects platelet function and thrombosis...
2016: PloS One
https://www.readbyqxmd.com/read/27716141/multi-omics-analysis-reveals-regulators-of-the-response-to-pdgf-bb-treatment-in-pulmonary-artery-smooth-muscle-cells
#10
Jidong Chen, Xiaolei Cui, Zhengjiang Qian, Yanjiao Li, Kang Kang, Junle Qu, Li Li, Deming Gou
BACKGROUND: Pulmonary arterial hypertension (PAH) is a lethal disease with pronounced narrowing of pulmonary vessels due to abnormal cell proliferation. The platelet-derived growth factor BB (PDGF-BB) is well known as a potent mitogen for smooth muscle cell proliferation. To better understand how this growth factor regulates pulmonary arterial smooth muscle cells (PASMCs) proliferation, we sought to characterize the response to PDGF-BB stimulation at system-wide levels, including the transcriptome and proteome...
October 6, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27713681/mass-spectrometry-based-approaches-to-targeted-quantitative-proteomics-in-cardiovascular-disease
#11
Clementina Mesaros, Ian A Blair
Mass spectrometry-based proteomics methodology has become an important tool in elucidating some of the underlying mechanisms involved in cardiovascular disease. The present review provides details on selected important protein targets where highly selective and specific mass spectrometry-based approaches have led to important new findings and provided new mechanistic information. The role of six proteins involved in the etiology of cardiovascular disease (acetylated platelet cyclooxygenase-1, serum apolipoprotein A1, apolipoprotein C-III, serum C-reactive protein, serum high mobility group box-1 protein, insulin-like growth factor I) and their quantification has been discussed...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27708557/plasma-degradome-affected-by-variable-storage-of-human-blood
#12
Maria Kaisar, Leon F A van Dullemen, Marie-Laëtitia Thézénas, M Zeeshan Akhtar, Honglei Huang, Sandrine Rendel, Philip D Charles, Roman Fischer, Rutger J Ploeg, Benedikt M Kessler
BACKGROUND: The successful application of-omics technologies in the discovery of novel biomarkers and targets of therapeutic interventions is facilitated by large collections of well curated clinical samples stored in bio banks. Mining the plasma proteome holds promise to improve our understanding of disease mechanisms and may represent a source of biomarkers. However, a major confounding factor for defining disease-specific proteomic signatures in plasma is the variation in handling and processing of clinical samples leading to protein degradation...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27686810/proteogenomics-for-the-study-of-gastrointestinal-stromal-tumors
#13
Tadashi Kondo
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the gastrointestinal tract. Gain-of-function mutations in KIT or platelet-derived growth factor receptor alpha (PDGFRA) drive most GISTs, and 85 % of GISTs also contain oncogenic mutations in one of two receptor tyrosine kinases. The advent of tyrosine kinase inhibitors has had a significant impact on the clinical practices for GISTs. However, tumors in more than 80 % of GIST patients acquire resistance against treatments with tyrosine kinase inhibitors; thus, driver mechanisms of secondary resistance as well as biomarkers for early detection of recurrence have been explored for better clinical outcomes...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27676445/bottom-up-proteomics-suggests-an-association-between-differential-expression-of-mitochondrial-proteins-and-chronic-fatigue-syndrome
#14
F Ciregia, L Kollipara, L Giusti, R P Zahedi, C Giacomelli, M R Mazzoni, G Giannaccini, P Scarpellini, A Urbani, A Sickmann, A Lucacchini, L Bazzichi
Chronic fatigue syndrome (CFS) is a debilitating and complex disorder characterized by unexplained fatigue not improved by rest. An area of investigation is the likely connection of CFS with defective mitochondrial function. In a previous work, we investigated the proteomic salivary profile in a couple of monozygotic twins discordant for CFS. Following this work, we analyzed mitochondrial proteins in the same couple of twins. Nano-liquid chromatography electrospray ionization mass spectrometry (nano-LC-MS) was used to study the mitochondria extracted from platelets of the twins...
September 27, 2016: Translational Psychiatry
https://www.readbyqxmd.com/read/27669021/salivary-gland-proteome-during-adult-development-and-after-blood-feeding-of-female-anopheles-dissidens-mosquitoes-diptera-culicidae
#15
Benjarat Phattanawiboon, Narissara Jariyapan, Chonlada Mano, Sittiruk Roytrakul, Atchara Paemanee, Sriwatapron Sor-Suwan, Patchara Sriwichai, Atiporn Saeung, Paul A Bates
Understanding changes in mosquito salivary proteins during the time that sporozoite maturation occurs and after blood feeding may give information regarding the roles of salivary proteins during the malarial transmission. Anopheles dissidens (formerly Anopheles barbirostris species A1) is a potential vector of Plasmodium vivax in Thailand. In this study, analyses of the proteomic profiles of female An. dissidens salivary glands during adult development and after blood feeding were carried out using two-dimensional gel electrophoresis coupled with nano-liquid chromatography-mass spectrometry...
2016: PloS One
https://www.readbyqxmd.com/read/27621705/photosensitivity-to-triflusal-formation-of-a-photoadduct-with-ubiquitin-demonstrated-by-photophysical-and-proteomic-techniques
#16
Edurne Nuin, Dolores Pérez-Sala, Virginie Lhiaubet-Vallet, Inmaculada Andreu, Miguel A Miranda
Triflusal is a platelet aggregation inhibitor chemically related to acetylsalicylic acid, which is used for the prevention and/or treatment of vascular thromboembolisms, which acts as a prodrug. Actually, after oral administration it is absorbed primarily in the small intestine, binds to plasma proteins (99%) and is rapidly biotransformed in the liver into its deacetylated active metabolite 2-hydroxy-4-trifluoromethylbenzoic acid (HTB). In healthy humans, the half-life of triflusal is ca. 0.5 h, whereas for HTB it is ca...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27602567/towards-a-proteomic-catalogue-and-differential-annotation-of-salivary-gland-proteins-in-blood-fed-malaria-vector-anopheles-culicifacies-by-mass-spectrometry
#17
Ritu Rawal, Sonam Vijay, Kavita Kadian, Jagbir Singh, Veena Pande, Arun Sharma
In order to understand the importance of functional proteins in mosquito behavior, following blood meal, a baseline proteomic dataset is essential for providing insights into the physiology of blood feeding. Therefore, in this study as first step, in solution and 1-D electrophoresis digestion approach combined with tandem mass spectrometry (nano LC-MS/MS) and computational bioinformatics for data mining was used to prepare a baseline proteomic catalogue of salivary gland proteins of sugar fed An. culicifacies mosquitoes...
2016: PloS One
https://www.readbyqxmd.com/read/27601597/comprehensive-proteome-profiling-of-platelet-identified-a-protein-profile-predictive-of-responses-to-an-antiplatelet-agent-sarpogrelate
#18
Hangyeore Lee, Sehyun Chae, Jisook Park, Jingi Bae, Eun-Bi Go, Su-Jin Kim, Hokeun Kim, Daehee Hwang, Sang-Won Lee, Soo-Youn Lee
Sarpogrelate is an antiplatelet agent widely used to treat arterial occlusive diseases. Evaluation of platelet aggregation is essential to monitor therapeutic effects of sarpogrelate. Currently, no molecular signatures are available to evaluate platelet aggregation. Here, we performed comprehensive proteome profiling of platelets collected from 18 subjects before and after sarpogrelate administration using LC-MS/MS analysis coupled with extensive fractionation. Of 5423 proteins detected, we identified 499 proteins affected by sarpogrelate and found that they strongly represented cellular processes related to platelet activation and aggregation, including cell activation, coagulation, and vesicle-mediated transports...
November 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27589083/an-efficient-method-for-isolation-of-representative-and-contamination-free-population-of-blood-platelets-for-proteomic-studies
#19
Aneta Wrzyszcz, Joanna Urbaniak, Agnieszka Sapa, Mieczysław Woźniak
To date, there has been no ideal method for blood platelet isolation which allows one to obtain a preparation devoid of contaminations, reflecting the activation status and morphological features of circulating platelets. To address these requirements, we have developed a method which combines the continuous density gradient centrifugation with washing from PGI2-supplemented platelet-rich plasma (PRP). We have assessed the degree of erythrocyte and leukocyte contamination, recovery of platelets, morphological features, activation status, and reactivity of isolated platelets...
September 2, 2016: Platelets
https://www.readbyqxmd.com/read/27540960/interactive-protein-network-of-fxiii-a1-in-lipid-rafts-of-activated-and-non-activated-platelets
#20
Vahideh Rabani, Damien Montange, Siamak Davani
Lipid-rafts are defined as membrane microdomains enriched in cholesterol and glycosphingolipids within platelet plasma membrane. Lipid raft-mediated clot retraction requires factor XIII and other interacting proteins. The aim of this study was to investigate the proteins that interact with factor XIII in raft and non-raft domains of activated and non-activated platelet plasma membrane. By lipidomics analysis, we identified cholesterol- and sphingomyelin-enriched areas as lipid rafts. Platelets were activated by thrombin...
September 2016: Platelets
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