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Platelet Proteomics

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https://www.readbyqxmd.com/read/29242851/in-depth-ptdins-3-4-5-p3-signalosome-analysis-identifies-dapp1-as-a-negative-regulator-of-gpvi-driven-platelet-function
#1
Tom N Durrant, James L Hutchinson, Kate J Heesom, Karen E Anderson, Len R Stephens, Phillip T Hawkins, Aaron J Marshall, Samantha F Moore, Ingeborg Hers
The class I phosphoinositide 3-kinase (PI3K) isoforms play important roles in platelet priming, activation, and stable thrombus formation. Class I PI3Ks predominantly regulate cell function through their catalytic product, the signaling phospholipid phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P3], which coordinates the localization and/or activity of a diverse range of binding proteins. Notably, the complete repertoire of these class I PI3K effectors in platelets remains unknown, limiting mechanistic understanding of class I PI3K-mediated control of platelet function...
June 13, 2017: Blood Advances
https://www.readbyqxmd.com/read/29211968/men-and-women-differ-in-the-biochemical-composition-of-platelet-rich-plasma
#2
Grace Xiong, Nithya Lingampalli, Jayme C B Koltsov, Lawrence L Leung, Nidhi Bhutani, William H Robinson, Constance R Chu
BACKGROUND: Autologous platelet-rich plasma (PRP) is widely used for a variety of clinical applications. However, clinical outcome studies have not consistently shown positive effects. The composition of PRP differs based on many factors. An improved understanding of factors influencing the composition of PRP is important for the optimization of PRP use. HYPOTHESIS: Age and sex influence the PRP composition in healthy patients. STUDY DESIGN: Controlled laboratory study...
November 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29182335/the-proteomic-landscape-of-patient-derived-cd4-t-cells-in-recent-onset-type-1-diabetes
#3
Marlen F Lepper, Uli Ohmayer, Christine von Toerne, Nicole Maison, Anette-Gabriele Ziegler, Stefanie M Hauck
The pathophysiology underlying the autoimmune disease type 1 diabetes (T1D) is poorly understood. Obtaining an accurate proteomic profile of the T helper cell population is essential for understanding the pathogenesis of T1D. Here, we performed in-depth proteomic profiling of peripheral CD4+ T cells in a pediatric cohort in order to identify cellular signatures associated with the onset of T1D. Using only 250,000 CD4+ T cells per patient, isolated from biobanked PBMC samples, we identified nearly 6,000 proteins using deep-proteome profiling with LC-MS/MS data-independent acquisition...
November 28, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29167233/granzyme-a-in-human-platelets-regulates-the-synthesis-of-proinflammatory-cytokines-by-monocytes-in-aging
#4
Robert A Campbell, Zechariah Franks, Anish Bhatnagar, Jesse W Rowley, Bhanu K Manne, Mark A Supiano, Hansjorg Schwertz, Andrew S Weyrich, Matthew T Rondina
Dysregulated inflammation is implicated in the pathobiology of aging, yet platelet-leukocyte interactions and downstream cytokine synthesis in aging remains poorly understood. Platelets and monocytes were isolated from healthy younger (age <45, n = 37) and older (age ≥65, n = 30) adults and incubated together under autologous and nonautologous conditions. Synthesis of inflammatory cytokines by monocytes, alone or in the presence of platelets, was examined. Next-generation RNA-sequencing allowed for unbiased profiling of the platelet transcriptome in aging...
November 22, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29140295/quantification-of-cardiovascular-disease-biomarkers-in-human-platelets-by-targeted-mass-spectrometry
#5
Sebastian Malchow, Christina Loosse, Albert Sickmann, Christin Lorenz
Platelets are known to be key players in thrombosis and hemostasis, contributing to the genesis and progression of cardiovascular diseases. Due to their pivotal role in human physiology and pathology, platelet function is regulated tightly by numerous factors which have either stimulatory or inhibitory effects. A variety of factors, e.g., collagen, fibrinogen, ADP, vWF, thrombin, and thromboxane promote platelet adhesion and aggregation by utilizing multiple intracellular signal cascades. To quantify platelet proteins for this work, a targeted proteomics workflow was applied...
November 15, 2017: Proteomes
https://www.readbyqxmd.com/read/29130446/the-role-of-mucin-educated-platelet-activation-in-tumor-invasiveness-an-unfolding-concern-in-the-realm-of-cancer-biology
#6
EDITORIAL
Akbar Shoukat Ali, Arzoo Ajaz
Metastasis is a complex and well-coordinated phenotypic transformation of cancer cells governed by aberrant genetic and molecular pathways. It has been approved as the most consistent cause of cancer death. With emerging insight into the genomics, transcriptomics and proteomics, progress has been made and reasonably large number of molecular pathways of metastasis has been forwarded, but our understanding of precise underlying molecular mechanisms remains largely scarce. It has been well-known for around a decade and more that platelets are intriguingly contributing to the cancer metastasis...
December 2017: BioMedicine
https://www.readbyqxmd.com/read/29090039/platelet-carbonic-anhydrase-ii-a-forgotten-enzyme-may-be-responsible-for-aspirin-resistance
#7
M Jakubowski, J Dębski, E Szahidewicz-Krupska, A Turek-Jakubowska, J Gawryś, K Gawryś, R Skomro, A Derkacz, A Doroszko
BACKGROUND: Thromboembolic events constitute a major health problem, despite the steadily expanding arsenal of antiplatelet drugs. Hence, there is still a need to optimize the antiplatelet therapy. OBJECTIVES: The aim of our study was to verify a hypothesis that there are no differences in platelet proteome between two groups of healthy people representing different acetylsalicylic acid (aspirin) responses as assessed by the liquid chromatography/mass spectrometry (LC/MS) technique...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29053067/the-proteome-of-pus-from-human-brain-abscesses-host-derived-neurotoxic-proteins-and-the-cell-type-diversity-of-cns-pus
#8
Bjørnar Hassel, Gustavo Antonio De Souza, Maria Ekman Stensland, Jugoslav Ivanovic, Øyvind Voie, Daniel Dahlberg
OBJECTIVE What determines the extent of tissue destruction during brain abscess formation is not known. Pyogenic brain infections cause destruction of brain tissue that greatly exceeds the area occupied by microbes, as seen in experimental studies, pointing to cytotoxic factors other than microbes in pus. This study examined whether brain abscess pus contains cytotoxic proteins that might explain the extent of tissue destruction. METHODS Pus proteins from 20 human brain abscesses and, for comparison, 7 subdural empyemas were analyzed by proteomics mass spectrometry...
October 20, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29034237/proteomics-of-eosinophil-activation
#9
REVIEW
Deane F Mosher, Emily M Wilkerson, Keren B Turton, Alexander S Hebert, Joshua J Coon
We recently identified and quantified >7,000 proteins in non-activated human peripheral blood eosinophils using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and described phosphoproteomic changes that accompany acute activation of eosinophils by interleukin-5 (IL5) (1). These data comprise a treasure trove of information about eosinophils. We illustrate the power of label-free LC-MS/MS quantification by considering four examples: complexity of eosinophil STATs, contribution of immunoproteasome subunits to eosinophil proteasomes, complement of integrin subunits, and contribution of platelet proteins originating from platelet-eosinophil complexes to the overall proteome...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/28887518/monitoring-storage-induced-changes-in-the-platelet-proteome-employing-label-free-quantitative-mass-spectrometry
#10
Maaike Rijkers, Bart L van den Eshof, Pieter F van der Meer, Floris P J van Alphen, Dirk de Korte, Frank W G Leebeek, Alexander B Meijer, Jan Voorberg, A J Gerard Jansen
Shelf life of platelet concentrates is limited to 5-7 days due to loss of platelet function during storage, commonly referred to as the platelet storage lesion (PSL). To get more insight into the development of the PSL, we used label free quantitative mass spectrometry to identify changes in the platelet proteome during storage. In total 2501 proteins were accurately quantified in 3 biological replicates on at least 1 of the 7 different time-points analyzed. Significant changes in levels of 21 proteins were observed over time...
September 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821815/epac1-deficient-mice-have-bleeding-phenotype-and-thrombocytes-with-decreased-gpib%C3%AE-expression
#11
Gyrid Nygaard, Lars Herfindal, Kathrine S Asrud, Ronja Bjørnstad, Reidun K Kopperud, Eystein Oveland, Frode S Berven, Lene Myhren, Erling A Hoivik, Turid Helen Felli Lunde, Marit Bakke, Stein O Døskeland, Frode Selheim
Epac1 (Exchange protein directly activated by cAMP 1) limits fluid loss from the circulation by tightening the endothelial barrier. We show here that Epac1(-/-) mice, but not Epac2(-/-) mice, have prolonged bleeding time, suggesting that Epac1 may limit fluid loss also by restraining bleeding. The Epac1(-/-) mice had deficient in vitro secondary hemostasis. Quantitative comprehensive proteomics analysis revealed that Epac1(-/-) mouse platelets (thrombocytes) had unbalanced expression of key components of the glycoprotein Ib-IX-V (GPIb-IX-V) complex, with decrease of GP1bβ and no change of GP1bα...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811546/serum-derived-extracellular-vesicles-evs-impact-on-vascular-remodeling-and-prevent-muscle-damage-in-acute-hind-limb-ischemia
#12
Claudia Cavallari, Andrea Ranghino, Marta Tapparo, Massimo Cedrino, Federico Figliolini, Cristina Grange, Valentina Giannachi, Paolo Garneri, Maria Chiara Deregibus, Federica Collino, Pietro Rispoli, Giovanni Camussi, Maria Felice Brizzi
Serum is an abundant and accessible source of circulating extracellular vesicles (EVs). Serum-EV (sEV) pro-angiogenic capability and mechanisms are herein analyzed using an in vitro assay which predicts sEV angiogenic potential in vivo. Effective sEVs (e-sEVs) also improved vascular remodeling and prevented muscle damage in a mouse model of acute hind limb ischemia. e-sEV angiogenic proteomic and transcriptomic analyses show a positive correlation with matrix-metalloproteinase activation and extracellular matrix organization, cytokine and chemokine signaling pathways, Insulin-like Growth Factor and platelet pathways, and Vascular Endothelial Growth Factor signaling...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28762014/beyond-cox-1-the-effects-of-aspirin-on-platelet-biology-and-potential-mechanisms-of-chemoprevention
#13
Argentina Ornelas, Niki Zacharias-Millward, David G Menter, Jennifer S Davis, Lenard Lichtenberger, David Hawke, Ernest Hawk, Eduardo Vilar, Pratip Bhattacharya, Steven Millward
After more than a century, aspirin remains one of the most commonly used drugs in western medicine. Although mainly used for its anti-thrombotic, anti-pyretic, and analgesic properties, a multitude of clinical studies have provided convincing evidence that regular, low-dose aspirin use dramatically lowers the risk of cancer. These observations coincide with recent studies showing a functional relationship between platelets and tumors, suggesting that aspirin's chemopreventive properties may result, in part, from direct modulation of platelet biology and biochemistry...
July 31, 2017: Cancer Metastasis Reviews
https://www.readbyqxmd.com/read/28714000/circulating-levels-of-angiogenesis-related-growth-factors-in-breast-cancer-a-study-to-profile-proteins-responsible-for-tubule-formation
#14
Gemma A Barron, Marie Goua, Klaus W J Wahle, Giovanna Bermano
The present study exploited a versatile in vitro endothelial cell/fibroblast co-culture cell system to investigate the association between angiogenesis and breast cancer by comparing the capacity of plasma from women with breast cancer and age-matched controls, to influence tubule formation and modulate angiogenesis in vitro, and to identify plasma circulating factors which might be responsible. Plasma from women with breast cancer (n=8) (added on day 7 after co-culture establishment) significantly increased tubule formation by 57% (P<0...
September 2017: Oncology Reports
https://www.readbyqxmd.com/read/28712299/evaluation-of-efficacy-and-toxicity-of-exfoliated-silicate-nanoclays-as-a-feed-additive-for-fumonisin-detoxification
#15
Chiao-Wei Yuan, Jie-Ting Huang, Ching-Chin Chen, Pin-Chi Tang, Jenn-Wen Huang, Jiang-Jen Lin, San-Yuan Huang, Shuen-Ei Chen
The efficacy of nanosilicate clay platelets (NSCP), exfoliated silicates from natural montmorillonites, as a feed additive for ameliorating fumonisin B1 (FB1) toxicosis was evaluated. Toxicological mechanisms by NSCP were examined through proteomic and biochemical analyses. Dietary supplementation with NSCP at a low level of 40 mg/kg of feed improved growth performances in chickens with respect to FB1 toxicosis. Other issues of ameliorated symptoms including serum and/or hepatic aspartate aminotransferase activity, oxidative stress indicators, and sphinganine/sphingosine ratio, a hallmark of FB1 toxicosis, were considered...
August 9, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28649187/distinct-proteome-pathology-of-circulating-microparticles-in-systemic-lupus-erythematosus
#16
Ole Østergaard, Christoffer Tandrup Nielsen, Julia T Tanassi, Line V Iversen, Søren Jacobsen, Niels H H Heegaard
BACKGROUND: The pathogenesis of systemic lupus erythematosus (SLE) is poorly understood but has been linked to defective clearance of subcellular particulate material from the circulation. This study investigates the origin, formation, and specificity of circulating microparticles (MPs) in patients with SLE based on comprehensive MP proteome profiling using patients with systemic sclerosis (SSc) and healthy donors (HC) as controls. METHODS: We purified MPs from platelet-poor plasma using differential centrifugation of samples from SLE (n = 45), SSc (n = 38), and two sets of HC (n = 35, n = 25)...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28643538/do-methodological-differences-account-for-the-current-controversy-on-tissue-factor-expression-in-platelets
#17
Marta Brambilla, Laura Rossetti, Chiara Zara, Paola Canzano, Peter L A Giesen, Elena Tremoli, Marina Camera
Tissue factor (TF), the key activator of the blood coagulation cascade and of thrombus formation, is also expressed by circulating human platelets. Despite the documented in-depth characterization of platelet TF carried out in the past 15 years, some authors still fail to identify TF in platelets, especially when assessment in platelet-rich plasma (PRP) or washed platelets is carried out. This study aims to extend the characterization of the subset of TF-positive platelets in PRP from healthy subjects and to verify how different centrifugation forces, used to prepare the PRP, could affect the analysis of TF-positive platelets...
June 23, 2017: Platelets
https://www.readbyqxmd.com/read/28592188/polymer-coated-cardiopulmonary-bypass-circuit-attenuates-upregulation-of-both-proteases-protease-inhibitors-and-platelet-degranulation-in-pigs
#18
Shoichi Suehiro, Kouji Shimizu, Kensuke Imai, Atsushi Niii, Kazuhiro Akeho, Hayato Nakata, Akane Yamaguchi, Ken-Ichi Matsumoto, Teiji Oda
INTRODUCTION: Interaction of blood with a cardiopulmonary bypass (CPB) circuit activates the coagulation-fibrinolysis, complement and kinin-kallikrein systems that are mainly supported by proteases and their inhibitors. METHODS: Biocompatibility of a new polymer-coated (SEC-coated) CPB circuit was globally evaluated and compared with that of a non-coated CPB circuit by quantitative proteomics, using isobaric tags for relative and absolute quantification labeling tandem mass spectrometry...
June 1, 2017: Perfusion
https://www.readbyqxmd.com/read/28587468/human-platelet-vesicles-exhibit-distinct-size-and-proteome
#19
Bhanu P Jena, Paul M Stemmer, Sunxi Wang, Guangzhao Mao, Kenneth T Lewis, Daniel A Walz
In the past 50 years, isolated blood platelets have had restricted use in wound healing, cancer therapy, and organ and tissue transplant, to name a few. The major obstacle for its unrestricted use has been, among others, the presence of ultrahigh concentrations of growth factors and the presence of both pro-angiogenic and anti-angiogenic proteins. To overcome this problem requires the isolation and separation of the membrane bound secretory vesicles containing the different factors. In the current study, high-resolution imaging of isolated secretory vesicles from human platelets using atomic force microscopy (AFM) and mass spectrometry enabled characterization of the remaining vesicles size and composition following their immunoseparation...
June 9, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28576849/proteome-wide-identification-of-glycosylation-dependent-interactors-of-galectin-1-and-galectin-3-on-mesenchymal-retinal-pigment-epithelial-rpe-cells
#20
Jara Obermann, Claudia S Priglinger, Juliane Merl-Pham, Arie Geerlof, Sigfried Priglinger, Magdalena Götz, Stefanie M Hauck
Identification of interactors is a major goal in cell biology. Not only protein-protein but also protein-carbohydrate interactions are of high relevance for signal transduction in biological systems. Here, we aim to identify novel interacting binding partners for the β-galactoside-binding proteins galectin-1 (Gal-1) and galectin-3 (Gal-3) relevant in the context of the eye disease proliferative vitreoretinopathy (PVR). PVR is one of the most common failures after retinal detachment surgeries and is characterized by the migration, adhesion, and epithelial-to-mesenchymal transition of retinal pigment epithelial cells (RPE) and the subsequent formation of sub- and epiretinal fibrocellular membranes...
August 2017: Molecular & Cellular Proteomics: MCP
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