Katarina Kulhankova, Soumba Traore, Xue Cheng, Hadrien Benk-Fortin, Stéphanie Hallée, Mario Harvey, Joannie Roberge, Frédéric Couture, Sajeev Kohli, Thomas J Gross, David K Meyerholz, Garrett R Rettig, Bernice Thommandru, Gavin Kurgan, Christine Wohlford-Lenane, Dennis J Hartigan-O'Connor, Bradley P Yates, Gregory A Newby, David R Liu, Alice F Tarantal, David Guay, Paul B McCray
Gene editing strategies for cystic fibrosis are challenged by the complex barrier properties of airway epithelia. We previously reported that the amphiphilic S10 shuttle peptide non-covalently combined with CRISPR-associated (Cas) ribonucleoprotein (RNP) enabled editing of human and mouse airway epithelial cells. Here, we derive the S315 peptide as an improvement over S10 in delivering base editor RNP. Following intratracheal aerosol delivery of Cy5-labeled peptide in rhesus macaques, we confirm delivery throughout the respiratory tract...
December 5, 2023: Nature Communications