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https://www.readbyqxmd.com/read/28920748/investigation-of-the-transport-and-absorption-of-angelica-sinensis-polysaccharide-through-gastrointestinal-tract-both-in-vitro-and-in-vivo
#1
Kaiping Wang, Fang Cheng, Xianglin Pan, Tao Zhou, Xiqiu Liu, Ziming Zheng, Li Luo, Yu Zhang
To investigate the absorption and delivery of ASP in gastrointestinal (GI) tract, cASP was successfully synthesized by chemically modifying with succinic anhydride and then conjugating with a near infrared fluorescent dye Cy5.5. Then, the capacity of oral absorption of cASP was evaluated. The results demonstrated that cASP had low toxicity and no disruption on the integrity of cell membrane. The endocytosis of cASP into the epithelial cells was time- and energy-dependent, which was mediated by macropinocytosis pathway and clathrin- and caveolae (or lipid raft)-related routes...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28885119/volume-of-distribution-and-clearance-of-peptide-based-nanofiber-after-convection-enhanced-delivery
#2
Ranjodh Singh, Vanessa Bellat, Melinda Wang, Melanie E Schweitzer, Y Linda Wu, Ching-Hsuan Tung, Mark M Souweidane, Benedict Law
OBJECTIVE Drug clearance may be a limiting factor in the clinical application of convection-enhanced delivery (CED). Peptide-based nanofibers (NFPs) have a high aspect ratio, and NFPs loaded with drugs could potentially maintain effective drug concentrations for an extended period sufficient for cancer therapy. The objective of this study was to assess the volume of distribution (Vd) and clearance of variable lengths of NFPs when administered using CED. METHODS NFPs composed of multiple methoxypolyethylene glycol (mPEG)-conjugated constructs (mPEG2000-KLDLKLDLKLDL-K( FITC)-CONH2, for which FITC is fluorescein isothiocyanate) were assembled in an aqueous buffer...
September 8, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28882748/method-to-minimize-ozone-effect-on-cy5-fluorescent-intensity-in-dna-microarrays
#3
Youngjun Kim, Hyunhee Seo, Miseon Jeong, Kiheon Lee, Inho Lee, Kyeonga So, Mikyung Kim, Yookyung Lee, Seonah Kim, Taejin Kim
Cyanine 5 (Cy5) is an established fluorescent dye in microarray analysis. It is degraded rapidly when exposed to atmospheric ozone during post-hybridization washes, which leads to loss of fluorescent intensity. To minimize this undesirable effect, we coated microarray slides with sodium dodecyl sulfate (SDS) solution at post-hybridization washes. The fluorescent intensities on coated slides were more stable than those on uncoated slide. We also performed the microarrays with SDS solution for a year to check the solution's effectiveness along with seasonal changes of atmospheric ozone level...
September 4, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28881581/a-molecular-pathology-method-for-sequential-fluorescence-in-situ-hybridization-for-multi-gene-analysis-at-the-single-cell-level
#4
Linping Hu, Xiuxiu Yin, Jiangman Sun, Anders Zetterberg, Weimin Miao, Tao Cheng
Multi-gene detection at the single-cell level is desirable to enable more precise genotyping of heterogeneous hematology and oncology samples. This study aimed to establish a single-cell multi-gene fluorescence in situ hybridization (FISH) method for use in molecular pathology analyses. Five fluorochromes were used to label different FISH gene probes, and 5 genes were detected using a five-color FISH protocol. After the first hybridization, the previous FISH probe set was stripped, and a second set of five-color FISH probes was used for rehybridization...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28880092/using-peptide-aptamer-targeted-polymers-as-a-model-nanomedicine-for-investigating-drug-distribution-in-cancer-nanotheranostics
#5
Yongmei Zhao, Zachary H Houston, Joshua D Simpson, Liyu Chen, Nicholas L Fletcher, Adrian V Fuchs, Idriss Blakey, Kristofer J Thurecht
Theranostics is a strategy that combines multiple functions such as targeting, stimulus-responsive drug release, and diagnostic imaging into a single platform, often with the aim of developing personalized medicine.1,2 Based on this concept, several well-established hyperbranched polymeric theranostic nanoparticles were synthesized and characterized as model nanomedicines to investigate how their properties affect the distribution of loaded drugs at both the cell and whole animal levels. An 8-mer peptide aptamer was covalently bound to the periphery of the nanoparticles to achieve both targeting and potential chemosensitization functionality against heat shock protein 70 (Hsp70)...
September 7, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28874046/intracellular-proteolytic-disassembly-of-self-quenched-near-infrared-nanoparticles-turning-fluorescence-on-for-tumor-targeted-imaging
#6
Jinhui Jiang, Zhibin Zhao, Zijuan Hai, Hongyong Wang, Gaolin Liang
The design of tumor-targeting, intracellular protease-activatable near-infrared fluorescence (NIRF) nanoprobes is broadly interesting but remains challenging. In this work, we report the rational design of a NIR probe Cys(StBu)-Lys(Biotin)-Lys-Lys(Cy5.5)-CBT (1) to facilely prepare the self-quenched nanoparticles 1-NPs for tumor-targeted imaging in vitro and in vivo. The biotinylated 1-NPs could be actively uptaken by biotin receptor-overexpressing tumor cells via receptor-mediated endocytosis. Upon intracellular proteolytic cleavage, 1-NPs were disassembled to yield the small molecular probe Lys(Cy5...
September 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28873407/photo-affinity-labelling-and-biochemical-analyses-identify-the-target-of-trypanocidal-simplified-natural-product-analogues
#7
Lindsay B Tulloch, Stefanie K Menzies, Andrew L Fraser, Eoin R Gould, Elizabeth F King, Marija K Zacharova, Gordon J Florence, Terry K Smith
Current drugs to treat African sleeping sickness are inadequate and new therapies are urgently required. As part of a medicinal chemistry programme based upon the simplification of acetogenin-type ether scaffolds, we previously reported the promising trypanocidal activity of compound 1, a bis-tetrahydropyran 1,4-triazole (B-THP-T) inhibitor. This study aims to identify the protein target(s) of this class of compound in Trypanosoma brucei to understand its mode of action and aid further structural optimisation...
September 5, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28782357/beyond-a-carrier-graphene-quantum-dots-as-a-probe-for-programmatically-monitoring-anti-cancer-drug-delivery-release-and-response
#8
Hui Ding, Fan Zhang, Chaochao Zhao, Yanlin Lv, Guanghui Ma, Wei Wei, Zhiyuan Tian
On the basis of the unique physicochemical properties of graphene quantum dots (GQDs), we developed a novel type of theranostic agent by loading anticancer drug doxorubicin (DOX) to GQD's surface and conjugating Cy5.5 (Cy) dye to GQD though a cathepsin D-responsive (P) peptide. Such type of agents demonstrated superior therapeutic performance both in vitro and in vivo because of the improved tissue penetration and cellular uptake. More importantly, they are capable of functioning as probes for programmed tracking the delivery and release of anticancer drug as well as drug-induced cancer cell apoptosis through GQD's, DOX's, and Cy's charateristic fluorescence, respectively...
August 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28723206/evaluating-dye-labeled-dna-dendrimers-for-potential-applications-in-molecular-biosensing
#9
Carl W Brown, Susan Buckhout-White, Sebastián A Díaz, Joseph S Melinger, Mario G Ancona, Ellen R Goldman, Igor L Medintz
DNA nanostructures provide a reliable and predictable scaffold for precisely positioning fluorescent dyes to form energy transfer cascades. Furthermore, these structures and their attendant dye networks can be dynamically manipulated by biochemical inputs, with the changes reflected in the spectral response. However, the complexity of DNA structures that have undergone such types of manipulation for direct biosensing applications is quite limited. Here, we investigate four different modification strategies to effect such dynamic manipulations using a DNA dendrimer scaffold as a testbed, and with applications to biosensing in mind...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28610486/resveratrol-loaded-glycyrrhizic-acid-conjugated-human-serum-albumin-nanoparticles-wrapping-resveratrol-nanoparticles-preparation-characterization-and-targeting-effect-on-liver-tumors
#10
Mingfang Wu, Bolin Lian, Yiping Deng, Ziqi Feng, Chen Zhong, Weiwei Wu, Yannian Huang, Lingling Wang, Chang Zu, Xiuhua Zhao
In this study, glycyrrhizic acid-conjugated human serum albumin nanoparticles wrapping resveratrol nanoparticles were prepared to establish a tumor targeting nano-sized drug delivery system. Glycyrrhizic acid was coupled to human serum albumin, and resveratrol was encapsulated in glycyrrhizic acid-conjugated human serum albumin by high-pressure homogenization emulsification. The average particle size of sample nanoparticles prepared under the optimal conditions was 108.1 ± 5.3 nm with a polydispersity index (PDI) of 0...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28608939/multivalent-siderophore-dotam-conjugates-as-theranostics-for-imaging-and-treatment-of-bacterial-infections
#11
Kevin Ferreira, Hai-Yu Hu, Verena Fetz, Hans Prochnow, Bushra Rais, Peter P Müller, Mark Brönstrup
There is a strong need to better diagnose infections at deep body sites through noninvasive molecular imaging methods. Herein, we describe the synthesis and characterization of probes based on siderophore conjugates with catechol moieties and a central DOTAM scaffold. The probes can accommodate a metal ion as well as an antibiotic moiety and are therefore suited for theranostic purposes. The translocation of the conjugates across the outer and inner cell membranes of E. coli was confirmed by growth recovery experiments with enterobactin-deficient strains, by the antibacterial activity of ampicillin conjugates, and by confocal imaging using a fluorogen-activating protein-malachite green system adapted to E...
June 13, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28592123/sorafenib-inhibits-growth-of-hepatoma-with-hypoxia-and-hypoxia-driven-angiogenesis-in-nude-mice
#12
F Li, F Wang, J Wu
The study aimed to investigate the effects of sorafenib on growth of hepatoma with hypoxia and angiogenesis. Green fluorescent protein (GFP)-expressing SMMC-7721 hepatoma cells were established via transfection with a lentiviral vector carrying GFP. GFP-labelled cells were treated with CoCl2 for 24 h with or without pretreatment of sorafenib for 30 min, and then injected subcutaneously into nude mice to induce hepatoma xenografts. This study used 16 BALB/c nude mice, which were divided into 4 groups: control group (GFP-labelled cells), CoCl2 group (GFP-labelled cells treated with CoCl2), CoCl2 plus sorafenib group (GFP-labelled cells pretreated with sorafenib and then treated with CoCl2) and CoCl2 plus sorafenib (cell+i...
June 8, 2017: Neoplasma
https://www.readbyqxmd.com/read/28589972/the-influence-of-tumor-induced-immune-dysfunction-on-the-immune-cell-distribution-of-gold-nanoparticles-in-vivo
#13
Chenxi Wu, Hongmei Chen, Xuan Wu, Xiuxiu Cong, Li Wang, Yucai Wang, Yongguang Yang, Wei Li, Tianmeng Sun
Gold nanoparticles (AuNPs) have been extensively explored as a drug carrier and have been widely used to provide advanced biomedical research tools in diagnostic imaging and therapy for cancer. Although the mononuclear phagocyte system and immune system are known to play the main roles in the clearance of AuNPs during the circulation, the particle distribution within the immune cells under the condition of immune dysfunction caused by tumor growth has not been thoroughly studied. Here, the cellular distribution of Cy5 labeled AuNPs with diameters of 5, 30 and 50 nm is characterized within the immune populations of the blood, spleen and bone marrow from tumor free and tumor bearing mice using flow cytometry...
July 25, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28582716/combination-of-active-targeting-enzyme-triggered-release-and-fluorescent-dye-into-gold-nanoclusters-for-endomicroscopy-guided-photothermal-photodynamic-therapy-to-pancreatic-ductal-adenocarcinoma
#14
Hui Li, Ping Wang, Yunxiang Deng, Meiying Zeng, Yan Tang, Wei-Hong Zhu, Yingsheng Cheng
Pancreatic ductal adenocarcinoma (PDAC) is one of the most devastating malignancies in patients, and there is an urgent need for an effective treatment method. Herein, we report a novel gold nanocluster-based platform for confocal laser endomicroscopy-guided photothermal therapy (PTT)/photodynamic therapy (PDT) for PDAC, which consists of four components: the PTT-carrier gold nanocluster, an active targeting ligand U11 peptide, a Cathepsin E (CTSE)-sensitive PDT therapy prodrug, and a CTSE-sensitive imaging agent (cyanine dye Cy5...
September 2017: Biomaterials
https://www.readbyqxmd.com/read/28582715/in%C3%A2-vivo-stem-cell-tracking-with-imageable-nanoparticles-that-bind-bioorthogonal-chemical-receptors-on-the-stem-cell-surface
#15
Sangmin Lee, Hwa In Yoon, Jin Hee Na, Sangmin Jeon, Seungho Lim, Heebeom Koo, Sang-Soo Han, Sun-Woong Kang, Soon-Jung Park, Sung-Hwan Moon, Jae Hyung Park, Yong Woo Cho, Byung-Soo Kim, Sang Kyoon Kim, Taekwan Lee, Dongkyu Kim, Seulki Lee, Martin G Pomper, Ick Chan Kwon, Kwangmeyung Kim
It is urgently necessary to develop reliable non-invasive stem cell imaging technology for tracking the in vivo fate of transplanted stem cells in living subjects. Herein, we developed a simple and well controlled stem cell imaging method through a combination of metabolic glycoengineering and bioorthogonal copper-free click chemistry. Firstly, the exogenous chemical receptors containing azide (-N3) groups were generated on the surfaces of stem cells through metabolic glycoengineering using metabolic precursor, tetra-acetylated N-azidoacetyl-d-mannosamine(Ac4ManNAz)...
September 2017: Biomaterials
https://www.readbyqxmd.com/read/28546913/genetic-assembly-of-double-layered-fluorescent-protein-nanoparticles-for-cancer-targeting-and-imaging
#16
Seong-Eun Kim, Sung Duk Jo, Koo Chul Kwon, You-Yeon Won, Jeewon Lee
Hepatitis B virus capsid (HBVC), a self-assembled protein nanoparticle comprised of 180 or 240 subunit proteins, is used as a cage for genetic encapsulation of fluorescent proteins (FPs). The self-quenching of FPs is controlled by varying the spacing between FPs within the capsid structure. Double-layered FP nanoparticle possessing cancer cell-targeting capabilities is also produced by additionally attaching FPs and cancer cell receptor-binding peptides (affibodies) to the outer surface of the capsid. The generically modified HBVC with double layers of mCardinal FPs and affibodies (mC-DL-HBVC) exhibit a high fluorescence intensity and a strong photostability, and is efficiently internalized by cancer cells and significantly stable against intracellular degradation...
May 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28524656/multifunctional-and-redox-responsive-self-assembled-magnetic-nanovectors-for-protein-delivery-and-dual-modal-imaging
#17
Hong Yu Yang, Moon-Sun Jang, Yi Li, Jung Hee Lee, Doo Sung Lee
Nanoparticle (NP) based model carriers present an emerging strategy for protein delivery. However, constructing a multifunctional nanocarrier with high loading capacity, diagnostic imaging capacity, and controlled release capability is a tremendous challenge for protein delivery systems. Thus, we herein report on the fabrication of redox-responsive magnetic nanovectors (termed RMNs) through self- assembly of Fe3O4 NPs and redox-responsive polymer ligands, which could effectively transport protein and trigger intracellular protein release...
June 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28513559/detecting-and-discriminating-shigella-sonnei-using-an-aptamer-based-fluorescent-biosensor-platform
#18
Myeong-Sub Song, Simranjeet Singh Sekhon, Woo-Ri Shin, Hyung Cheol Kim, Jiho Min, Ji-Young Ahn, Yang-Hoon Kim
In this paper, a Whole-Bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Shigella sonnei. Real-time PCR amplification and post-SELEX experiment revealed that the selected aptmers possessed a high binding affinity and specificity for S. sonnei. Of the 21 aptamers tested, the C(t) values of the SS-3 and SS-4 aptamers (Ct = 13.89 and Ct = 12.23, respectively) had the lowest value compared to other aptamer candidates. The SS-3 and SS-4 aptamers also displayed a binding affinity (KD) of 39...
May 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28503066/essential-oil-mediated-glycerosomes-increase-transdermal-paeoniflorin-delivery-optimization-characterization-and-evaluation-in-vitro-and-in-vivo
#19
Kai Zhang, Yongtai Zhang, Zhe Li, Nana Li, Nianping Feng
In this study, a novel glycerosome carrier containing essential oils was prepared for topical administration of paeoniflorin (PF) to enhance its transdermal drug delivery and improve drug absorption in the synovium. The formulation of glycerosomes was optimized by a uniform design, and the final vehicle was composed of 5% (w/v) phospholipid, 0.6% (w/v) cholesterol, and 10% (v/v) glycerol, with 2% (v/v) Speranskia tuberculata essential oil (STO) as the transdermal enhancer. The in vitro transdermal flux of PF loaded in the STO-glycerosomes was 1...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28480924/a-comparative-study-of-plasmonic-enhanced-single-molecule-fluorescence-induced-by-gold-nanoantennas-and-its-application-for-illuminating-telomerase
#20
Caixia Niu, Manshu Peng, Ying You, Ruihua Wang, Yijing Jia, Tianxin Xie, Jinyu Wang, Na Na, Jin Ouyang
A comparative study of plasmonic-enhanced single-molecule fluorescence (PESMF) induced by four gold nanoantennas is reported. The gold triangular nanoplate (Au TNP) is the optimal PESMF substrate for Cy5.5 owing to its sharpest point and strongest electric fields. The Au TNP is chosen for the preparation of a telomerase sensor.
May 8, 2017: Chemical Communications: Chem Comm
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