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https://www.readbyqxmd.com/read/29311548/author-correction-targeted-gadofullerene-for-sensitive-magnetic-resonance-imaging-and-risk-stratification-of-breast-cancer
#1
Zheng Han, Xiaohui Wu, Sarah Roelle, Chuheng Chen, William P Schiemann, Zheng-Rong Lu
In the original version of this Article, the penultimate sentence of the Abstract incorrectly read 'The dose of the contrast agent for effective molecular MRI is only slightly lower than that of ZD2-Cy5.5 (0.5 µmol kg-1) in fluorescence imaging.' The correct version states 'higher' in place of 'lower'. This error has been corrected in both the PDF and HTML versions of the Article.
January 8, 2018: Nature Communications
https://www.readbyqxmd.com/read/29237264/dual-ratiometric-target-triggered-fluorescent-probe-for-simultaneous-quantitative-visualization-of-tumor-microenvironment-protease-activity-and-ph-in-vivo
#2
Tiancong Ma, Yi Hou, Jianfeng Zeng, Chunyan Liu, Peisen Zhang, Lihong Jing, Dihua Shangguan, Mingyuan Gao
The abnormal expression of tumor-associated proteases and lowered extracellular pH are important signatures strongly associated with cancer invasion, progression, and metastasis. However, their malignant effects were mainly identified using cell and tissue studies. To non-invasively visualize the heterogeneous distribution of these abnormal indicators in vivo and further disclose their collective behaviors, a target-triggered fluorescent nanoprobe composed of a ratiometric pH-sensitive dye, a near infrared dye (Cy5...
December 14, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29195018/dendron-grafted-polylysine-based-dual-modal-nanoprobe-for-ultra-early-diagnosis-of-pancreatic-precancerosis-via-targeting-a-urokinase-type-plasminogen-activator-receptor
#3
Hui Li, Ping Wang, Wenyu Gong, Qi Wang, Jia Zhou, Wei-Hong Zhu, Yingsheng Cheng
Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes of cancer death. Early detection of precancerous pancreatic intraepithelial neoplasia (PanIN) tissues is an urgent challenge to improve the PDAC prognosis. Here, a urokinase-type plasminogen activator receptor (uPAR)-targeted magnetic resonance (MR)/near-infrared fluorescence (NIRF) dual-modal nanoprobe dendron-grafted polylysine (DGL)-U11 for ultra-early detection of pancreatic precancerosis is reported. Because of its good biocompatibility and biodegradability, globular architecture, and well-defined reactive groups, the DGL is chosen as the platform to load with a pancreatic tumor-targeting peptide U11, a magnetic resonance contrast agent Gd3+ -diethylene triamine pentaacetic acid, and a near-infrared fluorescent cyanine dye Cy5...
December 1, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29192289/caspase-3-7-specific-metabolic-precursor-for-bioorthogonal-tracking-of-tumor-apoptosis
#4
Man Kyu Shim, Hong Yeol Yoon, Sangmin Lee, Mun Kyeong Jo, Jooho Park, Jong-Ho Kim, Seo Young Jeong, Ick Chan Kwon, Kwangmeyung Kim
Apoptosis is one of the most important intracellular events in living cell, which is a programmed cell death interrelated with caspase enzyme activity for maintaining homeostasis in multicellular organisms. Therefore, direct apoptosis imaging of living cells can provide enormous advantages for diagnosis, drug discovery, and therapeutic monitoring in various diseases. However, a method of direct apoptosis imaging has not been fully validated, especially for live cells in in vitro and in vivo. Herein, we developed a new apoptosis imaging technology via a direct visualization of active caspase-3/-7 activity in living cells...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29185197/a-non-peptidic-s100a9-specific-ligand-for-optical-imaging-of-phagocyte-activity-in-vivo
#5
Tom Völler, Andreas Faust, Johannes Roth, Michael Schäfers, Thomas Vogl, Sven Hermann
PURPOSE: Non-invasive assessment of inflammatory activity in the course of various diseases is a largely unmet clinical challenge. An early feature of inflammation is local secretion of the alarmin S100A8/A9 by activated phagocytes. We here evaluate a novel S100A9-targeted small molecule tracer Cy5.5-CES271 for in vivo optical imaging of inflammatory activity in exemplary disease models. PROCEDURES: Dynamics of Cy5.5-CES271 was characterized in a model of irritant contact dermatitis by sequential fluorescence reflectance imaging (FRI) up to 24 h postinjection (p...
November 28, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29171939/initial-flow-cytometric-evaluation-of-the-clearllab-lymphoid-screen
#6
B D Hedley, G Cheng, J Luider, W Kern, G Lozanski, I Chin-Yee, L E Lowes, M Keeney, D Careaga, R Magari, L Tejidor
INTRODUCTION: Flow cytometric immunophenotyping (FCI) is an integral part in the diagnosis and classification of hematologic malignancies. FCI results also influence therapeutic decisions and disease prognosis. ClearLLab LS is a 12-antibody 10-color cocktail provided in dry format designed as a screen for patients suspected of having hematolymphoid disease. METHODS: A blinded comparison between ClearLLab LS, (CD8-FITC,Kappa-FITC,CD4-PE,Lambda-PE,CD19-ECD,CD56-PE-Cy5...
November 24, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29156758/development-of-novel-long-noncoding-rna-malat1-near-infrared-optical-probes-for-in-vivo-tumour-imaging
#7
Meng-Jie Dong, Cai-Qin Wang, Guo-Lin Wang, Yue-Hong Wang, Zhen-Feng Liu
With the advent of next-generation sequencing technology, there is rapidly increasing interest in long noncoding RNAs (lncRNAs). The objectives of this study were to develop a novel lncRNA MALAT1 near-infrared optical probe, to evaluate the characteristics of this optical imaging probe in vitro and to determine whether it can be used for imaging MALAT1 expression in malignant tumours in vivo. Conjugation of Cy5.5 to MALAT1 ASO was accomplished using standard NHS (N-hydroxysuccinimide) ester procedures, and the labelled MALAT1 ASO was purified with a Glen-Pak DNA Purification Cartridge and reversed-phase high performance liquid chromatography (HPLC)...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29152080/vincristine-liposomes-with-smaller-particle-size-have-stronger-diffusion-ability-in-tumor-and-improve-tumor-accumulation-of-vincristine-significantly
#8
Siyu Ma, Mingyuan Li, Nan Liu, Ying Li, Zhiping Li, Yang Yang, Fanglin Yu, Xiaoqin Hu, Cheng Liu, Xingguo Mei
The passive targeting is the premise of active targeting that could make nanocarrier detained in tumor tissue. The particle size is the most important factor that influences the diffusion and distribution of nanoparticle both in vivo and in vitro. In order to investigate the relationship between particle size and diffusion ability, two kinds of liposome loaded with Vincristine (VCR-Lip) were prepared. The diffusion behavior of VCR-Lip with different particle size and free VCR was compared through diffusion stability study...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29152066/identification-and-validation-of-fgfr2-peptide-for-detection-of-early-barrett-s-neoplasia
#9
Juan Zhou, Lei He, Zhijun Pang, Henry D Appelman, Rork Kuick, David G Beer, Meng Li, Thomas D Wang
The incidence of esophageal adenocarcinoma (EAC) is rising rapidly, and early detection within the precursor state of Barrett's esophagus (BE) is challenged by flat premalignant lesions that are difficult detect with conventional endoscopic surveillance. Overexpression of cell surface fibroblast growth factor receptor 2 (FGFR2) is an early event in progression of BE to EAC, and is a promising imaging target. We used phage display to identify the peptide SRRPASFRTARE that binds specifically to the extracellular domain of FGFR2...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29108201/-clinical-features-of-imbalance-between-th1-and-th22-cells-and-its-association-with-disease-progression-in-patients-with-liver-cirrhosis
#10
H Q Wu, J J Zhao, H W Li, Z Zhang
Objective: To investigate the clinical features of imbalance between Th1 and Th22 cells and its association with disease progression in patients with liver cirrhosis, and to explore immune therapeutic strategies for targeted therapy for liver cirrhosis. Methods: In vitro peripheral blood mononucleated cells (PBMCs) were collected by centrifugation. CD3-BV500 and CD8-PerCP-Cy5.5 staining was performed for these cells. IFNγ-PE-Cy7, IL-17a-APC, IL-22-PE, or the corresponding isotype control was added, and then PBMCs were fixed with 1% polyoxymethylene after being washed once by permeabilization-wash buffer...
October 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/29101636/lyp-1-conjugated-nanoparticles-for-magnetic-resonance-imaging-of-triple-negative-breast-cancer
#11
Abedelnasser Abulrob, Slavisa Corluka, Barbara Blasiak, B Gino Falonne, Dragana Ponjevic, John Matyas, Boguslaw Tomanek
PURPOSE: Triple-negative breast cancer (TNBC) does not express estrogen receptor, progesterone receptor, or Her2/neu. Both diagnosis and treatment of TNBC remain a clinical challenge. LyP-1 is a cyclic 9 amino acid peptide that can bind to breast cancer cells. The goal of this study was to design and characterize LyP-1 conjugated to fluorescent iron oxide nanoparticles (LyP-1-Fe3O4-Cy5.5) as a contrast agent for improved and specific magnetic resonance imaging (MRI) in a preclinical model of TNBC...
November 3, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29101222/structural-basis-for-the-interaction-of-diapause-hormone-with-its-receptor-in-the-silkworm-bombyx-mori
#12
Zhangfei Shen, Xue Jiang, Lili Yan, Yu Chen, Weiwei Wang, Ying Shi, Liangen Shi, Dongxiang Liu, Naiming Zhou
Diapause hormone (DH) is a 24-aa amidated neuropeptide that elicits the embryonic diapause of the silkworm, Bombyx mori (Bommo), via sensitive and selective interaction with its receptor, Bommo DH receptor (Bommo-DHR). Previous studies of the structure-activity relationship of Bommo-DH were all based on an in vivo diapause-induction bioassay, which has provided little information on the structure of Bommo-DHR or its iteration with DH. Here, to unveil the interaction of Bommo-DH with its receptor, N-terminally truncated analogs and alanine-scanning mutants of Bommo-DH were chemically synthesized and functionally evaluated by using a Cy5...
November 3, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29080502/cross-linked-magnetic-nanoparticles-with-a-biocompatible-amide-bond-for-cancer-targeted-dual-optical-magnetic-resonance-imaging
#13
Hee-Man Yang, Chan Woo Park, Sungjune Park, Jong-Duk Kim
A poly(succinimide) (PSI) graft copolymer was designed and synthesized as a cross-linkable precursor polymer to fabricate biocompatible and biodegradable cross-linked magnetic nanoparticles (CMNPs) with excellent structural stability in vivo and multifunctionality, including specific cancer-targeting and dual imaging modalities. After coating the magnetic nanoparticles with amphiphilic PSI grafted with folate-conjugated PEG and alkyl chains, the succinimide units on the inner shell of the nanoparticles were cross-linked and converted into a biocompatible and biodegradable structure consisting of amide bonds and further used to bear free amine groups on the surface of the CMNPs...
October 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29066894/poly-d-l-lactic-glycolic-acid-star-glucose-nanoparticles-for-glucose-transporter-and-hypoglycemia-mediated-tumor-targeting
#14
Ju-Hwan Park, Hyun-Jong Cho, Dae-Duk Kim
Poly((D,L)lactic-glycolic)acid-star glucose (PLGA-Glc) polymer-based nanoparticles (NPs) were fabricated for tumor-targeted delivery of docetaxel (DCT). NPs with an approximate mean diameter of 241 nm, narrow size distribution, negative zeta potential, and spherical shape were prepared. A sustained drug release pattern from the developed NPs was observed for 13 days. Moreover, drug release from PLGA-Glc NPs at acidic pH (endocytic compartments and tumor regions) was significantly improved compared with that observed at physiological pH (normal tissues and organs)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29063765/directional-photonic-wire-mediated-by-homo-f%C3%A3-rster-resonance-energy-transfer-on-a-dna-origami-platform
#15
Francesca Nicoli, Anders Barth, Wooli Bae, Fabian Neukirchinger, Alvaro H Crevenna, Don C Lamb, Tim Liedl
Elaborating efficient strategies and deepening the understanding of light transport at the nanoscale is of great importance for future designs of artificial light-harvesting assemblies and dye-based photonic circuits. In this work, we focus on studying the phenomenon of Förster resonance energy transfer (FRET) among fluorophores of the same kind (homo-FRET) and its implications for energy cascades containing two or three different dye molecules. Utilizing the spatial programmability of DNA origami, we arranged a chain of cyanine 3 (Cy3) dyes flanked at one end with a dye of lower excitation energy, cyanine 5 (Cy5), with or without an additional dye of higher excitation energy, Alexa488, at the other end...
November 1, 2017: ACS Nano
https://www.readbyqxmd.com/read/29042222/development-of-a-microarray-based-assay-for-efficient-testing-of-new-hsp70-dnak-inhibitors
#16
Sona Mohammadi-Ostad-Kalayeh, Vjaceslavs Hrupins, Sabine Helmsen, Frank Stahl, Thomas Scheper, Matthias Preller, Frank Surup, Marc Stadler, Andreas Kirschning, Carsten Zeilinger
A facile method for testing ATP binding in a highly miniaturized microarray environment using human HSP70 and DnaK from Mycobacterium tuberculosis as biological targets is reported. Supported by molecular modelling studies we demonstrate that the position of the fluorescence label on ATP has a strong influence on the binding to human HSP70. Importantly, the label has to be positioned on the adenine ring and not to the terminal phosphate group. Unlabelled ATP displaced bound Cy5-ATP from HSP70 in the micromolar range...
October 7, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29025987/preclinical-development-of-cd38-targeted-89zr-zr-dfo-daratumumab-for-imaging-multiple-myeloma
#17
Anchal Ghai, Dolonchampa Maji, Nicholas Cho, Chantiya Chanswangphuwana, Michael Rettig, John DiPersio, Walter Akers, Farrokh Dehdashti, Samuel Achilefu, Ravi Vij, Monica Shokeen
Multiple myeloma (MM) is a plasma B-cell hematologic cancer that causes significant skeletal morbidity. Despite improvements in survival, heterogeneity in response remains a major challenge in MM. Cluster of differentiation 38 (CD38) is a type II transmembrane glycoprotein over-expressed in myeloma cells and is implicated in MM cell signaling. Daratumumab is US Food and Drug Administration approved high-affinity monoclonal antibody targeting CD38 that is clinically benefiting refractory MM patients. Here, we evaluated [89Zr]Zr-DFO-daratumumab positron emission tomography/computed tomography (PET/CT) imaging in MM tumor models...
October 12, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28987884/co-delivery-of-autophagy-inhibitor-atg7-sirna-and-docetaxel-for-breast-cancer-treatment
#18
Chunai Gong, Chuling Hu, Fenfen Gu, Qingming Xia, Chong Yao, Lijuan Zhang, Lei Qiang, Shen Gao, Yuan Gao
The lysosomal degradation pathway of autophagy has a crucial role in protecting cancer cells from multiple endogenous and exogenous stresses, particularly during the pathogenesis of cancer. Accordingly, agents that inhibit autophagy may have broad therapeutic applications. We have developed a novel strategy based on co-delivery of an autophagy related 7 (ATG7) siRNA and docetaxel (DTX) in a crosslinked, reducible, peptide-based micellar system for breast cancer treatment. Our results show that DTX and siATG7 co-treatment exhibited 2...
October 5, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28986275/quantification-of-resident-work-in-colorectal-surgery
#19
Elizabeth A Bailey, Adam P Johnson, Ira L Leeds, Rachel L Medbery, Vanita Ahuja, Thomas VanderMeer, Elizabeth C Wick, Busayo Irojah, Rachel R Kelz
OBJECTIVE: To examine resident intraoperative participation, perceived autonomy, and communication patterns between residents and attending surgeons using a novel survey tool. DESIGN: This was a prospective multi-institutional study. Operative residents completed the survey tool immediately after each colorectal resection performed during the study period. Resident intraoperative participation was quantified including degree of involvement in the technical aspects of the case, self-perception of autonomy, and communication strategies between the resident and attending...
October 3, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/28982548/cyanine-derivative-as-a-suitable-marker-for-thermosensitive-in-situ-gelling-delivery-systems-in-vitro-and-in-vivo-validation-of-a-sustained-buccal-drug-delivery
#20
Ni Zeng, Johanne Seguin, Pierre-Louis Destruel, Gilles Dumortier, Marc Maury, Hélène Dhotel, Michel Bessodes, Daniel Scherman, Nathalie Mignet, Vincent Boudy
Buccal administration route is a promising way for a large number of drugs exhibiting a low oral bioavailability. The present work describes the formulation and evaluation of a mucoadhesive and thermosensitive in situ gelling delivery system based on poloxamer 407, poloxamer 188 and xanthan gum for buccal drug delivery. First, the mucoadhesion properties were evaluated using a tensile test. The effect of xanthan gum on the mucoadhesion force was demonstrated. Then, to assess the buccal residence time which reflects the mucoadhesion properties, the validation of a fluorescence probe for in vivo optical imaging experiment was conducted...
October 2, 2017: International Journal of Pharmaceutics
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