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https://www.readbyqxmd.com/read/28288894/endogenous-inspired-biomineral-installed-hyaluronan-nanoparticles-as-ph-responsive-carrier-of-methotrexate-for-rheumatoid-arthritis
#1
Md Mahmudul Alam, Hwa Seung Han, Shijin Sung, Jin Hee Kang, Keum Hee Sa, Hasan Al Faruque, Jungwan Hong, Eon Jeong Nam, In San Kim, Jae Hyung Park, Young Mo Kang
Methotrexate (MTX), an anchor drug for rheumatoid arthritis (RA), has been suffered from refractoriness and high toxicity limiting effective dosage. To mitigate these challenges, the ability to selectively deliver MTX to arthritis tissue is a much sought-after modality for the treatment of RA. In this study, we prepared mineralized nanoparticles (MP-HANPs), composed of PEGylated hyaluronic acid (P-HA) as the hydrophilic shell, 5β-cholanic acid as the hydrophobic core, and calcium phosphate (CaP) as the pH-responsive mineral...
March 11, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28257990/extracellular-matrix-remodeling-in-vivo-for-enhancing-tumor-targeting-efficiency-of-nanoparticle-drug-carriers-using-the-pulsed-high-intensity-focused-ultrasound
#2
Sangmin Lee, Hyounkoo Han, Heebeom Koo, Jin Hee Na, Hong Yeol Yoon, Kyung Eun Lee, Hyukjin Lee, Hyuncheol Kim, Ick Chan Kwon, Kwangmeyung Kim
Dense and stiff extracellular matrix (ECM) in heterogeneous tumor tissues can inhibit deep penetration of nanoparticle drug carriers and decreases their therapeutic efficacy. Herein, we suggest the ECM remodeling strategy by the pulsed high intensity focused ultrasound (Pulsed-HIFU) technology for enhanced tumor-targeting of nanoparticles. First, we clearly observed that the tumor-targeting efficacy and tissue penetration of intravenously injected Cy5.5-labled glycol chitosan nanoparticles (Cy5.5-CNPs) were greatly inhibited in tumor tissue containing high collagen and hyaluronan contents in ECM-rich A549 tumor-bearing mice, compared to in ECM-less SCC7...
February 28, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28252391/visualizing-epithelial-expression-in-vertical-and-horizontal-planes-with-dual-axes-confocal-endomicroscope-using-compact-distal-scanner
#3
Gaoming Li, Haijun Li, Xiyu Duan, Quan Zhou, Juan Zhou, Thomas Wang
The epithelium is a thin layer of tissue that lines hollow organs, such as colon. Visualizing in vertical cross-sections with sub-cellular resolution is essential to understanding early disease mechanisms that progress naturally in the plane perpendicular to the tissue surface. The dual axes confocal architecture collects optical sections in tissue by directing light at an angle incident to the surface using separate illumination and collection beams to reduce effects of scattering, enhance dynamic range, and increase imaging depth...
February 23, 2017: IEEE Transactions on Medical Imaging
https://www.readbyqxmd.com/read/28247764/application-of-spectral-crosstalk-correction-for-improving-multiplexed-microrna-detection-using-a-single-excitation-wavelength
#4
Yuanjian Liu, Min Wei, Ying Li, Anran Liu, Wei Wei, Yuanjian Zhang, Songqin Liu
MicroRNAs (miRNAs) play crucial roles in the regulation of cellular activities and are next-generation biomarkers for early cancer detection. Simultaneous monitoring of multiplexed miRNA is very important for enhancing the accuracy of cancer diagnostics. Traditional fluorescence methods for multicomponent analysis were usually operated under multiple excitation wavelengths, because spectral crosstalk is very detrimental to detecting accuracy for multicomponent analysis. Herein, we present a fluorescence strategy for multi-miRNAs detection in plasma under a single excitation wavelength...
March 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28179099/hydrophobically-modified-polysaccharide-based-on-polysialic-acid-nanoparticles-as-carriers-for-anticancer-drugs
#5
Bom Jung, Man-Kyu Shim, Min-Ju Park, Eun Hyang Jang, Hong Yeol Yoon, Kwangmeyung Kim, Jong-Ho Kim
This study presented the development of hydrophobically modified polysialic acid (HPSA) nanoparticles, a novel anticancer drug nanocarrier that increases therapeutic efficacy without causing nonspecific toxicity towards normal cells. HPSA nanoparticles were prepared by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS) coupling between N-deacetylated polysialic acid (PSA) and 5β-cholanic acid. The physicochemical characteristics of HPSA nanoparticles (zeta-potential, morphology and size) were measured, and in vitro cytotoxicity and cellular uptake of PSA and HPSA nanoparticles were tested in A549 cells...
February 4, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28167355/development-of-a-new-bioactivatable-fluorescent-probe-for-quantification-of-apolipoprotein-a-i-proteolytic-degradation-in%C3%A2-vitro-and-in%C3%A2-vivo
#6
Foued Maafi, Baoqiang Li, Catherine Gebhard, Mathieu R Brodeur, Walid Nachar, Louis Villeneuve, Frédéric Lesage, David Rhainds, Eric Rhéaume, Jean-Claude Tardif
BACKGROUND AND AIMS: The potential benefits of high-density lipoproteins (HDL) against atherosclerosis are attributed to its major protein component, apolipoprotein A-I (apoA-I). Most of the apoA-I in the vascular wall appears to be in its lipid-poor form. The latter, however, is subjected to degradation by proteases localized in atherosclerotic plaques, which, in turn, has been shown to negatively impact its atheroprotective functions. Here, we report the development and in vivo use of a bioactivatable near-infrared full-length apoA-I-Cy5...
March 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28150208/designing-multicomponent-nanosystems-for-rapid-detection-of-circulating-tumor-cells
#7
Shashwat S Banerjee, Vrushali Khobragade, Jayant Khandare
Detection of circulating tumor cells (CTCs) in the blood circulation holds immense promise as it predicts the overall probability of patient survival. Therefore, CTC-based technologies are gaining prominence as a "liquid biopsy" for cancer diagnostics and prognostics. Here, we describe the design and synthesis of two distinct multicomponent magnetic nanosystems for rapid capture and detection of CTCs. The multifunctional Magneto-Dendrimeric Nano System (MDNS) composed of an anchoring dendrimer that is conjugated to multiple agents such as near infrared (NIR) fluorescent cyanine 5 NHS (Cy5), glutathione (GSH), transferrin (Tf), and iron oxide (Fe3O4) magnetic nanoparticle (MNP) for simultaneous tumor cell-specific affinity, multimodal high resolution confocal imaging, and cell isolation...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28116362/a-rapid-albumin-binding-5-fluorouracil-prodrug-with-a-prolonged-circulation-time-and-enhanced-antitumor-activity
#8
Dongyang Zhao, Huicong Zhang, Wenhui Tao, Wei Wei, Jin Sun, Zhonggui He
5-Fluorouracil (5-FU) is an antimetabolite widely used in the treatment of a variety of solid tumors. However, its clinical applications are greatly hindered by a very short residence time in blood circulation and non-specific distribution in the body. In order to overcome these challenges, 1-alkylcarbonyloxymethyl 5-FU was designed and linked with a maleimide group to form an albumin-binding 5-FU prodrug, named EMC-5-FU. In vitro incubation with bovine serum albumin (BSA) and fresh rat blood proved that the prodrug bound rapidly to cysteine-34 to form the drug-albumin conjugate nanomedicine...
January 24, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28098756/detection-of-the-vascular-endothelial-growth-factor-with-a-novel-bioluminescence-resonance-energy-transfer-pair-using-a-two-component-system
#9
Tobias Wimmer, Eva Schroeter, Birgit Lorenz, Knut Stieger
In this paper we describe a two-component BRET (bioluminescence resonance energy transfer)-based method to detect vascular endothelial growth factor (VEGF) molecules in unknown samples as the basis for subsequent in vivo use. A luminescent VEGF binding molecule, which binds in the receptor binding motif of VEGF, is used as the energy donor, transferred to a fluorophore-coupled VEGF binding molecule (acceptor), which binds to the neuropilin binding motif of VEGF, thus enabling energy transfer from the donor to the acceptor molecule...
January 13, 2017: Sensors
https://www.readbyqxmd.com/read/28063892/an-intraocular-drug-delivery-system-using-targeted-nanocarriers-attenuates-retinal-ganglion-cell-degeneration
#10
Lei Zhao, Guojun Chen, Jun Li, Yingmei Fu, Timur A Mavlyutov, Annie Yao, Robert W Nickells, Shaoqin Gong, Lian-Wang Guo
Glaucoma is a common blinding disease characterized by loss of retinal ganglion cells (RGCs). To date, there is no clinically available treatment directly targeting RGCs. We aim to develop an RGC-targeted intraocular drug delivery system using unimolecular micelle nanoparticles (unimNPs) to prevent RGC loss. The unimNPs were formed by single/individual multi-arm star amphiphilic block copolymer poly(amidoamine)-polyvalerolactone-poly(ethylene glycol) (PAMAM-PVL-PEG). While the hydrophobic PAMAM-PVL core can encapsulate hydrophobic drugs, the hydrophilic PEG shell provides excellent water dispersity...
February 10, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28042324/mapping-sentinel-lymph-node-metastasis-by-dual-probe-optical-imaging
#11
Xiangyu Yang, Zhe Wang, Fuwu Zhang, Guizhi Zhu, Jibin Song, Gao-Jun Teng, Gang Niu, Xiaoyuan Chen
Purpose: Sentinel lymph node biopsy (SLNB) has emerged as the preferred standard procedure in patients with breast cancer, melanoma and other types of cancer. Herein, we developed a method to intra-operatively map SLNs and differentiate tumor metastases within SLNs at the same time, with the aim to provide more accurate and real-time intraoperative guidance. Experimental Design: Hyaluronic acid (HA), a ligand of lymphatic vessel endothelial hyaluronan receptor (LYVE)-1, is employed as a SLN mapping agent after being conjugated with a near-infrared fluorophore (Cy5...
2017: Theranostics
https://www.readbyqxmd.com/read/28032446/magnetic-resonance-imaging-of-intra-pancreatic-ductal-nanoparticle-delivery-to-islet-cells
#12
Ping Wang, Alana Ross, Byunghee Yoo, Jingsheng Yang, Christian Farrar, Chongzhao Ran, Pamela Pantazopoulos, Zdravka Medarova, Anna Moore
The absence of reliable drug delivery systems to pancreatic beta-cells hampers efficient treatment of type 1 diabetes. Nanoparticle delivery systems equipped with imaging capabilities could enable selective delivery to pancreatic islet cells. Biodistribution of nanoparticles is defined by several factors including the mode of administration, which determines accumulation in various organs. In this study we tested whether intra-pancreatic ductal injection of magnetic nanoparticles (MN) would result in efficient cellular uptake by pancreatic islet cells...
December 29, 2016: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/27997133/injectable-guest-host-assembled-polyethylenimine-hydrogel-for-sirna-delivery
#13
Leo L Wang, Janna N Sloand, Ann C Gaffey, Chantel M Venkataraman, Zhichun Wang, Alen Trubelja, Daniel A Hammer, Pavan Atluri, Jason A Burdick
While siRNA has tremendous potential for therapeutic applications, advancement is limited by poor delivery systems. Systemically, siRNAs are rapidly degraded, may have off-target silencing, and necessitate high working concentrations. To overcome this, we developed an injectable, guest-host assembled hydrogel between polyethylenimine (PEI) and polyethylene glycol (PEG) for local siRNA delivery. Guest-host modified polymers assembled with siRNAs to form polyplexes that had improved transfection and viability compared to PEI...
December 20, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/27886058/optical-aptamer-probes-of-fluorescent-imaging-to-rapid-monitoring-of-circulating-tumor-cell
#14
Ji Yeon Hwang, Sang Tae Kim, Ho-Seong Han, Kyunggon Kim, Jin Soo Han
Fluorescence detecting of exogenous EpCAM (epithelial cell adhesion molecule) or muc1 (mucin1) expression correlated to cancer metastasis using nanoparticles provides pivotal information on CTC (circulating tumor cell) occurrence in a noninvasive tool. In this study, we study a new skill to detect extracellular EpCAM/muc1 using quantum dot-based aptamer beacon (QD-EpCAM/muc1 ALB (aptamer linker beacon). The QD-EpCAM/muc1 ALB was designed using QDs (quantum dots) and probe. The EpCAM/muc1-targeting aptamer contains a Ep-CAM/muc1 binding sequence and BHQ1 (black hole quencher 1) or BHQ2 (black hole quencher2)...
November 23, 2016: Sensors
https://www.readbyqxmd.com/read/27874037/visualizing-epithelial-expression-of-egfr-in-vivo-with-distal-scanning-side-viewing-confocal-endomicroscope
#15
Xiyu Duan, Haijun Li, Juan Zhou, Quan Zhou, Kenn R Oldham, Thomas D Wang
Confocal endomicroscopy is an emerging imaging technology that has recently been introduced into the clinic to instantaneously collect "optical biopsies" in vivo with histology-like quality. Here, we demonstrate a fast scanner located in the distal end of a side-viewing instrument using a compact lens assembly with numerical aperture of 0.5 to achieve a working distance of 100 μm and field-of-view of 300 × 400 μm(2). The microelectromechanical systems (MEMS) mirror was designed based on the principle of parametric resonance and images at 5 frames per second...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27862961/identification-of-a-glypican-3-binding-peptide-for-in-vivo-non-invasive-human-hepatocellular-carcinoma-detection
#16
Zainen Qin, Jingjing Wang, Ye Wang, Guohao Wang, Xiangyu Wang, Zhiyang Zhou, Gang Liu, Shi Gao, Lei Zhu
Early and accurate detection of hepatocellular carcinoma (HCC) is essential to improve the prognosis of patients and reduce the morbidity of surgical therapy. Glypican-3 (GPC3) is a protein abnormally expressed in HCC that has been identified as a serological and histochemical HCC marker. A novel peptide that specifically recognizes GPC3 will facilitate early detection of HCC and guide the treatment strategy. Herein, phage display screening technology is utilized to obtain a GPC3 binding peptide (GBP) using HCC cells expressing GPC3 in varying abundances...
November 15, 2016: Macromolecular Bioscience
https://www.readbyqxmd.com/read/27816727/flow-cytometric-analysis-of-t-lymphocyte-proliferation-in-vivo-by-edu-incorporation
#17
Xiaojing Sun, Chunpan Zhang, Hua Jin, Guangyong Sun, Yue Tian, Wen Shi, Dong Zhang
Monitoring T lymphocyte proliferation, especially in vivo, is essential for the evaluation of adaptive immune reactions. Flow cytometry-based proliferation assays have advantages in measuring cell division of different T lymphocyte subsets at the same time by multicolor labelling. In this study, we aimed to establish the use of 5-Ethynyl-2'-deoxyuridine (EdU) incorporation in vivo to monitor T lymphocyte proliferation by flow cytometry with an adoptive transfer model. We found that fixation followed by permeabilization preserved T cell surface antigens and had no obvious effects on the fluorescence intensity of APC, PE, PE-Cy7, FITC and PerCP-Cy5...
December 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27788352/mri-optical-dual-modality-imaging-of-vulnerable-atherosclerotic-plaque-with-an-osteopontin-targeted-probe-based-on-fe3o4-nanoparticles
#18
Hongyu Qiao, Yabin Wang, Ruohan Zhang, Quansheng Gao, Xiao Liang, Lei Gao, Zhenhua Jiang, Ruirui Qiao, Dong Han, Yan Zhang, Ya Qiu, Jie Tian, Mingyuan Gao, Feng Cao
Rupture of vulnerable atherosclerotic plaque is the major pathological cause of luminal thrombosis in acute coronary syndromes. Since foamy macrophages have been identified as a prominent component in vulnerable atherosclerotic lesions and osteopontin (OPN) is reported to be highly expressed in foamy macrophages, OPN could be a potential target for vulnerable atherosclerotic plaque imaging. The current study designed an OPN-specific MRI/optical dual-modality probe to detect vulnerable plaques. Fluorescence imaging revealed that 24 h after injection of the Cy5...
October 11, 2016: Biomaterials
https://www.readbyqxmd.com/read/27776554/s100a8-a9-a-potent-serum-and-molecular-imaging-biomarker-for-synovial-inflammation-and-joint-destruction-in-seronegative-experimental-arthritis
#19
Edwin J W Geven, Martijn H J van den Bosch, Irene Di Ceglie, Giuliana Ascone, Shahla Abdollahi-Roodsaz, Annet W Sloetjes, Sven Hermann, Michael Schäfers, Fons A J van de Loo, Peter M van der Kraan, Marije I Koenders, Dirk Foell, Johannes Roth, Thomas Vogl, Peter L E M van Lent
BACKGROUND: Seronegative joint diseases are characterized by a lack of well-defined biomarkers since autoantibodies are not elevated. Calprotectin (S100A8/A9) is a damage-associated molecular pattern (DAMP) which is released by activated phagocytes, and high levels are found in seronegative arthritides. In this study, we investigated the biomarker potential of systemic and local levels of these S100 proteins to assess joint inflammation and joint destruction in an experimental model for seronegative arthritis...
October 24, 2016: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/27768026/combined-near-infrared-fluorescent-imaging-and-micro-computed-tomography-for-directly-visualizing-cerebral-thromboemboli
#20
Dong-Eog Kim, Jeong-Yeon Kim, Su-Kyoung Lee, Ju Hee Ryu, Ick Chan Kwon, Cheol-Hee Ahn, Kwangmeyung Kim, Dawid Schellingerhout
Direct thrombus imaging visualizes the root cause of thromboembolic infarction. Being able to image thrombus directly allows far better investigation of stroke than relying on indirect measurements, and will be a potent and robust vascular research tool. We use an optical imaging approach that labels thrombi with a molecular imaging thrombus marker - a Cy5.5 near-infrared fluorescent (NIRF) probe that is covalently linked to the fibrin strands of the thrombus by the fibrin-crosslinking enzymatic action of activated coagulation factor XIIIa during the process of clot maturation...
September 25, 2016: Journal of Visualized Experiments: JoVE
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